AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09008


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09008.t1 MTR_8g080850 97.561 82 2 0 31 112 1 82 1.92e-52 159
MS.gene09008.t1 MTR_8g080840 87.654 81 10 0 30 110 437 517 5.06e-42 144
MS.gene09008.t1 MTR_8g080870 86.076 79 11 0 30 108 409 487 2.06e-41 142
MS.gene09008.t1 MTR_8g080880 87.342 79 10 0 30 108 437 515 3.48e-41 142
MS.gene09008.t1 MTR_8g097220 88.095 84 9 1 30 112 434 517 6.30e-41 141
MS.gene09008.t1 MTR_8g080890 85.714 77 11 0 30 106 438 514 5.25e-37 130
MS.gene09008.t1 MTR_4g074030 69.863 73 21 1 29 100 434 506 1.10e-25 100
MS.gene09008.t1 MTR_4g073970 63.077 65 23 1 30 93 238 302 1.64e-20 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09008.t1 AT4G34880 69.444 72 21 1 30 100 390 461 3.34e-25 98.6
MS.gene09008.t1 AT4G34880 69.444 72 21 1 30 100 436 507 3.82e-25 98.6

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCTGACGGTGTGGATTCT+TGG 0.256519 4.4:+15169265 MS.gene09008:CDS
TTTAGTAGCATACTTGCTAT+TGG 0.285041 4.4:+15169384 MS.gene09008:CDS
CTTGCTATTGGAGGTTATCT+TGG 0.307365 4.4:+15169396 MS.gene09008:CDS
TTGGTCTTGGAAGCTTAGTT+TGG 0.311340 4.4:+15169284 MS.gene09008:CDS
ATGCTACTAAAGAATGAAAA+AGG 0.327148 4.4:-15169372 None:intergenic
CAGCCATTTGGAATTTGCTT+TGG 0.337732 4.4:+15169450 MS.gene09008:CDS
CCATTTGGAATTTGCTTTGG+AGG 0.347630 4.4:+15169453 MS.gene09008:CDS
ACGGTGTGGATTCTTGGTCT+TGG 0.417239 4.4:+15169271 MS.gene09008:CDS
GTTCCAGCAGGATATGAAAA+AGG 0.427483 4.4:+15169426 MS.gene09008:CDS
GTCTTGGAAGCTTAGTTTGG+AGG 0.439009 4.4:+15169287 MS.gene09008:CDS
TTGTAACTTCCAACTTCCTA+AGG 0.444018 4.4:-15169554 None:intergenic
TAAGCAATCTCAATCAACTT+TGG 0.456653 4.4:-15169492 None:intergenic
AATCCACACCGTCAGACAAC+GGG 0.474967 4.4:-15169260 None:intergenic
GCCATCACCCCTTAGGAAGT+TGG 0.476560 4.4:+15169545 MS.gene09008:CDS
ATTTGCTTTGGAGGTTTGAA+AGG 0.485064 4.4:+15169462 MS.gene09008:CDS
AGTAGCATACTTGCTATTGG+AGG 0.497454 4.4:+15169387 MS.gene09008:CDS
TCAGAAAGCCATCACCCCTT+AGG 0.498171 4.4:+15169538 MS.gene09008:CDS
TCTTGGAAGCTTAGTTTGGA+GGG 0.501942 4.4:+15169288 MS.gene09008:CDS
CTTGGAGTGATTGTTCCAGC+AGG 0.513725 4.4:+15169414 MS.gene09008:CDS
TCCAACTTCCTAAGGGGTGA+TGG 0.529966 4.4:-15169546 None:intergenic
CCTCCAAAGCAAATTCCAAA+TGG 0.542753 4.4:-15169453 None:intergenic
GGCGAGTGCCCGTTGTCTGA+CGG 0.543749 4.4:+15169252 MS.gene09008:CDS
TGTAACTTCCAACTTCCTAA+GGG 0.549765 4.4:-15169553 None:intergenic
TTTGCTTTGGAGGTTTGAAA+GGG 0.567329 4.4:+15169463 MS.gene09008:CDS
AATAAATAAACTTGATGCTG+TGG 0.588874 4.4:+15169344 MS.gene09008:CDS
GAATCCACACCGTCAGACAA+CGG 0.634170 4.4:-15169261 None:intergenic
GTGCCCGTTGTCTGACGGTG+TGG 0.634476 4.4:+15169257 MS.gene09008:CDS
GTAACTTCCAACTTCCTAAG+GGG 0.639313 4.4:-15169552 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAAATAAACTTGATGCTG+TGG + chr4.4:15169344-15169363 MS.gene09008:CDS 25.0%
! ATGCTACTAAAGAATGAAAA+AGG - chr4.4:15169375-15169394 None:intergenic 25.0%
TAAGCAATCTCAATCAACTT+TGG - chr4.4:15169495-15169514 None:intergenic 30.0%
!! TTTAGTAGCATACTTGCTAT+TGG + chr4.4:15169384-15169403 MS.gene09008:CDS 30.0%
TATGAAAAAGGTCAGCCATT+TGG + chr4.4:15169438-15169457 MS.gene09008:CDS 35.0%
TGTAACTTCCAACTTCCTAA+GGG - chr4.4:15169556-15169575 None:intergenic 35.0%
TTGTAACTTCCAACTTCCTA+AGG - chr4.4:15169557-15169576 None:intergenic 35.0%
!! ATTTGCTTTGGAGGTTTGAA+AGG + chr4.4:15169462-15169481 MS.gene09008:CDS 35.0%
!! TTTGCTTTGGAGGTTTGAAA+GGG + chr4.4:15169463-15169482 MS.gene09008:CDS 35.0%
CCTCCAAAGCAAATTCCAAA+TGG - chr4.4:15169456-15169475 None:intergenic 40.0%
GTAACTTCCAACTTCCTAAG+GGG - chr4.4:15169555-15169574 None:intergenic 40.0%
GTTCCAGCAGGATATGAAAA+AGG + chr4.4:15169426-15169445 MS.gene09008:CDS 40.0%
! CAGCCATTTGGAATTTGCTT+TGG + chr4.4:15169450-15169469 MS.gene09008:CDS 40.0%
! CCATTTGGAATTTGCTTTGG+AGG + chr4.4:15169453-15169472 MS.gene09008:CDS 40.0%
! TCTTGGAAGCTTAGTTTGGA+GGG + chr4.4:15169288-15169307 MS.gene09008:CDS 40.0%
!! AGTAGCATACTTGCTATTGG+AGG + chr4.4:15169387-15169406 MS.gene09008:CDS 40.0%
!! CTTGCTATTGGAGGTTATCT+TGG + chr4.4:15169396-15169415 MS.gene09008:CDS 40.0%
!! TGACCTTTTTCATATCCTGC+TGG - chr4.4:15169432-15169451 None:intergenic 40.0%
!! TTGGTCTTGGAAGCTTAGTT+TGG + chr4.4:15169284-15169303 MS.gene09008:CDS 40.0%
! GTCTTGGAAGCTTAGTTTGG+AGG + chr4.4:15169287-15169306 MS.gene09008:CDS 45.0%
AATCCACACCGTCAGACAAC+GGG - chr4.4:15169263-15169282 None:intergenic 50.0%
ACGGTGTGGATTCTTGGTCT+TGG + chr4.4:15169271-15169290 MS.gene09008:CDS 50.0%
CTTGGAGTGATTGTTCCAGC+AGG + chr4.4:15169414-15169433 MS.gene09008:CDS 50.0%
GAATCCACACCGTCAGACAA+CGG - chr4.4:15169264-15169283 None:intergenic 50.0%
TCAGAAAGCCATCACCCCTT+AGG + chr4.4:15169538-15169557 MS.gene09008:CDS 50.0%
TCCAACTTCCTAAGGGGTGA+TGG - chr4.4:15169549-15169568 None:intergenic 50.0%
TGTCTGACGGTGTGGATTCT+TGG + chr4.4:15169265-15169284 MS.gene09008:CDS 50.0%
GCCATCACCCCTTAGGAAGT+TGG + chr4.4:15169545-15169564 MS.gene09008:CDS 55.0%
GGCGAGTGCCCGTTGTCTGA+CGG + chr4.4:15169252-15169271 MS.gene09008:CDS 65.0%
GTGCCCGTTGTCTGACGGTG+TGG + chr4.4:15169257-15169276 MS.gene09008:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 15169249 15169587 15169249 ID=MS.gene09008
chr4.4 mRNA 15169249 15169587 15169249 ID=MS.gene09008.t1;Parent=MS.gene09008
chr4.4 exon 15169249 15169587 15169249 ID=MS.gene09008.t1.exon1;Parent=MS.gene09008.t1
chr4.4 CDS 15169249 15169587 15169249 ID=cds.MS.gene09008.t1;Parent=MS.gene09008.t1
Gene Sequence

>MS.gene09008

ATGGGCGAGTGCCCGTTGTCTGACGGTGTGGATTCTTGGTCTTGGAAGCTTAGTTTGGAGGGAGTGTACTCTGTTCGATCTGCATCTCTGATGAAAATAAATAAACTTGATGCTGTGGTAACACCTTTTTCATTCTTTAGTAGCATACTTGCTATTGGAGGTTATCTTGGAGTGATTGTTCCAGCAGGATATGAAAAAGGTCAGCCATTTGGAATTTGCTTTGGAGGTTTGAAAGGGTCAGAACCAAAGTTGATTGAGATTGCTTATTCATTTGAGCAAGCAACTTTGATCAGAAAGCCATCACCCCTTAGGAAGTTGGAAGTTACAAGTTTGAAGTAG

Protein sequence

>MS.gene09008.t1

MGECPLSDGVDSWSWKLSLEGVYSVRSASLMKINKLDAVVTPFSFFSSILAIGGYLGVIVPAGYEKGQPFGICFGGLKGSEPKLIEIAYSFEQATLIRKPSPLRKLEVTSLK