AlfalfaGEDB | MS.gene50653

M. sativa cultivar XinJiangDaYe / MS.gene50653

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50653.t1	AES89336.1	90.8	87	8	0	1	87	1	87	4.50E-38	166.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50653.t1	P33081	80.0	85	14	1	1	85	1	82	4.2e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50653.t1	G7JM93	90.8	87	8	0	1	87	1	87	3.3e-38	166.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene50653	0.812822	3.49E-51	-1.69E-46
MS.gene054184	MS.gene50653	0.819541	1.10E-52	-1.69E-46
MS.gene054185	MS.gene50653	0.823643	1.24E-53	-1.69E-46
MS.gene055133	MS.gene50653	0.82145	4.01E-53	-1.69E-46
MS.gene057754	MS.gene50653	0.808308	3.30E-50	-1.69E-46
MS.gene057794	MS.gene50653	0.801649	8.15E-49	-1.69E-46
MS.gene057796	MS.gene50653	0.908915	1.01E-81	-1.69E-46
MS.gene059692	MS.gene50653	0.803595	3.23E-49	-1.69E-46
MS.gene06037	MS.gene50653	0.827304	1.68E-54	-1.69E-46
MS.gene060476	MS.gene50653	0.803196	3.91E-49	-1.69E-46
MS.gene061049	MS.gene50653	0.817651	2.95E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene50653	MS.gene79885	PPI
MS.gene23372	MS.gene50653	PPI
MS.gene50659	MS.gene50653	PPI
MS.gene75316	MS.gene50653	PPI
MS.gene057790	MS.gene50653	PPI
MS.gene50653	MS.gene50764	PPI
MS.gene003624	MS.gene50653	PPI
MS.gene031201	MS.gene50653	PPI
MS.gene50653	MS.gene003577	PPI
MS.gene50653	MS.gene50654	PPI
MS.gene045362	MS.gene50653	PPI
MS.gene50675	MS.gene50653	PPI
MS.gene50653	MS.gene014670	PPI
MS.gene77523	MS.gene50653	PPI
MS.gene50653	MS.gene77524	PPI
MS.gene50653	MS.gene75314	PPI
MS.gene50653	MS.gene75679	PPI
MS.gene75674	MS.gene50653	PPI
MS.gene79890	MS.gene50653	PPI
MS.gene003615	MS.gene50653	PPI
MS.gene50653	MS.gene40325	PPI
MS.gene062973	MS.gene50653	PPI
MS.gene003602	MS.gene50653	PPI
MS.gene047517	MS.gene50653	PPI
MS.gene50653	MS.gene68760	PPI
MS.gene003627	MS.gene50653	PPI
MS.gene50653	MS.gene031198	PPI
MS.gene50653	MS.gene057797	PPI
MS.gene50653	MS.gene003614	PPI
MS.gene062984	MS.gene50653	PPI
MS.gene50653	MS.gene77528	PPI
MS.gene50653	MS.gene79886	PPI
MS.gene50653	MS.gene014671	PPI
MS.gene40327	MS.gene50653	PPI
MS.gene50653	MS.gene50765	PPI
MS.gene50653	MS.gene003596	PPI
MS.gene50653	MS.gene23371	PPI
MS.gene50653	MS.gene75672	PPI
MS.gene50653	MS.gene062974	PPI
MS.gene50653	MS.gene003583	PPI
MS.gene50653	MS.gene062951	PPI
MS.gene50653	MS.gene057801	PPI
MS.gene50653	MS.gene77527	PPI
MS.gene50653	MS.gene79891	PPI
MS.gene003587	MS.gene50653	PPI
MS.gene50653	MS.gene057795	PPI
MS.gene50653	MS.gene68162	PPI
MS.gene50653	MS.gene045367	PPI
MS.gene50653	MS.gene045364	PPI
MS.gene50653	MS.gene045363	PPI
MS.gene50653	MS.gene75675	PPI
MS.gene003590	MS.gene50653	PPI
MS.gene062943	MS.gene50653	PPI
MS.gene063112	MS.gene50653	PPI
MS.gene50653	MS.gene79887	PPI
MS.gene50653	MS.gene50652	PPI
MS.gene003608	MS.gene50653	PPI
MS.gene50653	MS.gene68761	PPI
MS.gene50653	MS.gene002380	PPI
MS.gene50653	MS.gene68165	PPI
MS.gene77526	MS.gene50653	PPI
MS.gene045360	MS.gene50653	PPI
MS.gene50653	MS.gene50684	PPI
MS.gene50766	MS.gene50653	PPI
MS.gene50653	MS.gene014668	PPI
MS.gene50653	MS.gene75315	PPI
MS.gene75677	MS.gene50653	PPI
MS.gene50653	MS.gene68164	PPI
MS.gene003625	MS.gene50653	PPI
MS.gene50653	MS.gene68759	PPI
MS.gene045361	MS.gene50653	PPI
MS.gene50653	MS.gene75676	PPI
MS.gene003591	MS.gene50653	PPI
MS.gene50653	MS.gene50687	PPI
MS.gene062979	MS.gene50653	PPI
MS.gene50653	MS.gene014223	PPI
MS.gene01358	MS.gene50653	PPI
MS.gene50653	MS.gene014669	PPI
MS.gene50653	MS.gene057808	PPI
MS.gene40326	MS.gene50653	PPI
MS.gene062952	MS.gene50653	PPI
MS.gene50676	MS.gene50653	PPI
MS.gene50653	MS.gene68163	PPI
MS.gene23370	MS.gene50653	PPI
MS.gene77529	MS.gene50653	PPI
MS.gene014667	MS.gene50653	PPI
MS.gene003594	MS.gene50653	PPI
MS.gene50665	MS.gene50653	PPI
MS.gene50653	MS.gene057818	PPI
MS.gene50677	MS.gene50653	PPI
MS.gene50653	MS.gene79889	PPI
MS.gene50653	MS.gene045368	PPI
MS.gene003586	MS.gene50653	PPI
MS.gene057778	MS.gene50653	PPI
MS.gene062988	MS.gene50653	PPI
MS.gene75673	MS.gene50653	PPI
MS.gene50653	MS.gene031200	PPI
MS.gene50653	MS.gene014222	PPI
MS.gene50653	MS.gene79888	PPI
MS.gene045366	MS.gene50653	PPI
MS.gene062963	MS.gene50653	PPI
MS.gene50653	MS.gene75678	PPI
MS.gene50653	MS.gene01764	PPI
MS.gene50653	MS.gene031199	PPI
MS.gene50763	MS.gene50653	PPI
MS.gene045365	MS.gene50653	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50653.t1	MTR_4g072660	90.805	87	8	0	1	87	1	87	4.35e-56	167
MS.gene50653.t1	MTR_4g072730	85.714	91	8	1	1	86	1	91	2.76e-54	163
MS.gene50653.t1	MTR_4g072620	83.696	92	10	1	1	87	1	92	1.20e-53	162
MS.gene50653.t1	MTR_4g072480	82.609	92	11	1	1	87	1	92	1.73e-52	159
MS.gene50653.t1	MTR_4g072680	79.348	92	14	1	1	87	1	92	1.17e-49	152
MS.gene50653.t1	MTR_4g072460	77.174	92	16	1	1	87	1	92	4.75e-48	147
MS.gene50653.t1	MTR_4g072500	76.087	92	17	1	1	87	1	92	2.04e-47	146
MS.gene50653.t1	MTR_4g072570	73.913	92	19	1	1	87	1	92	3.79e-46	143
MS.gene50653.t1	MTR_4g072530	75.556	90	17	1	1	85	1	90	5.66e-45	140
MS.gene50653.t1	MTR_4g072640	73.333	90	19	1	1	85	1	90	3.48e-44	138
MS.gene50653.t1	MTR_4g072310	68.817	93	23	1	1	87	1	93	9.84e-44	137
MS.gene50653.t1	MTR_4g072250	72.043	93	19	2	1	87	1	92	6.88e-43	134
MS.gene50653.t1	MTR_4g072860	68.817	93	23	1	1	87	1	93	7.25e-43	134
MS.gene50653.t1	MTR_4g072880	67.742	93	24	1	1	87	1	93	7.33e-43	134
MS.gene50653.t1	MTR_4g072270	66.667	93	25	1	1	87	1	93	1.76e-42	134
MS.gene50653.t1	MTR_4g072490	70.588	85	25	0	1	85	1	85	5.25e-42	132
MS.gene50653.t1	MTR_3g084150	66.304	92	26	1	1	87	1	92	2.38e-41	130
MS.gene50653.t1	MTR_4g072740	71.765	85	24	0	1	85	1	85	6.13e-41	129
MS.gene50653.t1	MTR_5g008360	79.452	73	15	0	15	87	11	83	1.35e-40	128
MS.gene50653.t1	MTR_4g072550	80.263	76	15	0	12	87	28	103	1.41e-40	129
MS.gene50653.t1	MTR_4g072240	65.934	91	25	1	1	85	1	91	1.46e-40	129
MS.gene50653.t1	MTR_5g008370	79.730	74	15	0	13	86	18	91	2.19e-40	130
MS.gene50653.t1	MTR_3g084240	66.304	92	26	1	1	87	1	92	2.44e-40	128
MS.gene50653.t1	MTR_3g084180	65.217	92	27	1	1	87	1	92	2.84e-40	128
MS.gene50653.t1	MTR_4g072220	65.934	91	25	1	1	85	1	91	3.41e-40	128
MS.gene50653.t1	MTR_4g072580	67.059	85	28	0	1	85	1	85	3.76e-40	127
MS.gene50653.t1	MTR_4g072600	68.235	85	27	0	1	85	1	85	5.29e-40	127
MS.gene50653.t1	MTR_3g084170	66.304	92	26	1	1	87	1	92	5.31e-40	127
MS.gene50653.t1	MTR_4g072470	69.412	85	26	0	1	85	1	85	5.69e-40	127
MS.gene50653.t1	MTR_8g461410	64.130	92	28	1	1	87	1	92	7.71e-40	127
MS.gene50653.t1	MTR_4g072230	65.591	93	24	2	1	87	1	91	9.12e-40	127
MS.gene50653.t1	MTR_4g072870	65.934	91	25	1	1	85	1	91	1.37e-39	126
MS.gene50653.t1	MTR_4g072300	62.637	91	28	1	1	85	1	91	6.09e-39	124
MS.gene50653.t1	MTR_4g072330	67.045	88	26	1	1	85	1	88	6.17e-39	124
MS.gene50653.t1	MTR_4g072850	67.391	92	23	1	1	85	1	92	7.34e-39	124
MS.gene50653.t1	MTR_4g072370	65.909	88	27	1	1	85	1	88	1.23e-38	124
MS.gene50653.t1	MTR_4g072630	67.442	86	27	1	1	85	1	86	1.70e-38	123
MS.gene50653.t1	MTR_4g072590	72.941	85	23	0	1	85	1	85	2.37e-38	123
MS.gene50653.t1	MTR_3g084220	65.217	92	27	1	1	87	1	92	4.23e-38	122
MS.gene50653.t1	MTR_3g117630	73.750	80	20	1	7	85	11	90	6.14e-38	122
MS.gene50653.t1	MTR_4g072610	71.765	85	23	1	1	85	1	84	7.90e-38	121
MS.gene50653.t1	MTR_4g072540	68.605	86	26	1	1	85	1	86	9.52e-38	121
MS.gene50653.t1	MTR_4g072890	62.637	91	28	1	1	85	1	91	1.11e-37	121
MS.gene50653.t1	MTR_4g050700	64.516	93	27	1	1	87	1	93	2.04e-37	120
MS.gene50653.t1	MTR_2g012410	64.516	93	27	1	1	87	1	93	2.04e-37	120
MS.gene50653.t1	MTR_4g072720	68.605	86	26	1	1	85	1	86	6.28e-37	119
MS.gene50653.t1	MTR_4g072380	68.605	86	26	1	1	85	1	86	6.53e-37	119
MS.gene50653.t1	MTR_4g072465	62.791	86	31	1	1	85	1	86	7.24e-37	119
MS.gene50653.t1	MTR_3g084260	65.591	93	26	2	1	87	1	93	9.08e-37	119
MS.gene50653.t1	MTR_3g084250	64.130	92	28	1	1	87	1	92	9.95e-37	119
MS.gene50653.t1	MTR_4g072830	64.368	87	28	1	1	84	1	87	1.33e-36	119
MS.gene50653.t1	MTR_4g072260	59.140	93	32	1	1	87	1	93	1.55e-36	119
MS.gene50653.t1	MTR_3g110038	63.333	90	28	1	1	85	1	90	1.61e-36	118
MS.gene50653.t1	MTR_3g084210	61.957	92	30	1	1	87	1	92	2.01e-36	118
MS.gene50653.t1	MTR_3g110042	66.667	87	24	1	1	82	1	87	3.22e-36	118
MS.gene50653.t1	MTR_4g072940	75.000	72	18	0	16	87	53	124	3.28e-36	119
MS.gene50653.t1	MTR_4g072820	67.059	85	28	0	1	85	1	85	4.62e-36	117
MS.gene50653.t1	MTR_4g072670	66.279	86	28	1	1	85	1	86	1.53e-35	115
MS.gene50653.t1	MTR_8g461420	61.957	92	30	1	1	87	1	92	2.22e-35	115
MS.gene50653.t1	MTR_3g084190	63.333	90	28	1	1	85	1	90	3.04e-35	115
MS.gene50653.t1	MTR_4g072650	65.882	85	28	1	1	85	1	84	3.08e-35	115
MS.gene50653.t1	MTR_4g072510	67.442	86	27	1	1	85	1	86	4.19e-35	115
MS.gene50653.t1	MTR_3g110032	59.140	93	32	1	1	87	1	93	7.96e-35	114
MS.gene50653.t1	MTR_3g084230	60.870	92	31	1	1	87	1	92	1.25e-34	114
MS.gene50653.t1	MTR_3g084160	60.870	92	31	1	1	87	1	92	1.96e-34	113
MS.gene50653.t1	MTR_3g110045	70.833	72	21	0	16	87	80	151	7.30e-34	114
MS.gene50653.t1	MTR_4g072690	68.235	85	27	0	1	85	1	85	1.59e-33	110
MS.gene50653.t1	MTR_4g072450	67.059	85	28	0	1	85	1	85	5.66e-33	109
MS.gene50653.t1	MTR_4g072980	59.091	88	35	1	1	87	8	95	9.60e-33	109
MS.gene50653.t1	MTR_5g008350	66.216	74	25	0	1	74	1	74	2.61e-32	107
MS.gene50653.t1	MTR_4g072770	63.529	85	26	2	1	85	1	80	5.83e-32	107
MS.gene50653.t1	MTR_4g072560	54.639	97	31	3	1	84	1	97	4.83e-31	105
MS.gene50653.t1	MTR_4g072290	56.322	87	30	1	1	87	1	79	1.15e-30	103
MS.gene50653.t1	MTR_3g117640	72.059	68	18	1	20	86	34	101	1.16e-30	104
MS.gene50653.t1	MTR_4g072910	65.672	67	23	0	19	85	34	100	3.51e-30	102
MS.gene50653.t1	MTR_4g072750	58.824	85	26	2	1	76	1	85	6.24e-29	99.4
MS.gene50653.t1	MTR_3g084200	62.500	72	22	1	1	67	1	72	3.46e-27	95.5
MS.gene50653.t1	MTR_3g110035	52.439	82	39	0	6	87	57	138	4.40e-27	96.3
MS.gene50653.t1	MTR_6g077850	53.731	67	31	0	19	85	33	99	1.98e-25	90.9
MS.gene50653.t1	MTR_3g113310	57.353	68	28	1	18	85	40	106	2.36e-25	90.9
MS.gene50653.t1	MTR_3g117610	59.701	67	26	1	18	84	43	108	1.75e-24	89.0
MS.gene50653.t1	MTR_4g072190	57.971	69	28	1	16	84	32	99	3.96e-24	87.8
MS.gene50653.t1	MTR_4g072280	52.000	75	30	1	1	69	1	75	4.07e-22	82.0
MS.gene50653.t1	MTR_4g072930	57.353	68	25	1	22	85	43	110	1.11e-21	81.6
MS.gene50653.t1	MTR_5g021820	51.515	66	31	1	19	84	39	103	1.51e-20	78.6
MS.gene50653.t1	MTR_8g076040	54.545	66	29	1	19	84	40	104	2.37e-20	78.2
MS.gene50653.t1	MTR_4g124750	48.485	66	33	1	19	84	68	132	8.32e-17	70.5
MS.gene50653.t1	MTR_4g072840	47.674	86	18	3	1	85	1	60	1.08e-16	67.8
MS.gene50653.t1	MTR_2g043960	48.485	66	33	1	19	84	75	139	1.27e-16	70.1
MS.gene50653.t1	MTR_7g118010	45.349	86	39	3	2	85	10	89	2.27e-16	68.2
MS.gene50653.t1	MTR_4g005310	50.000	66	32	1	16	80	58	123	3.96e-16	68.6
MS.gene50653.t1	MTR_8g461400	42.391	92	22	2	1	87	1	66	4.70e-16	66.2
MS.gene50653.t1	MTR_8g026730	50.000	68	31	2	20	85	27	93	8.62e-16	66.6
MS.gene50653.t1	MTR_1g024085	55.000	60	26	1	19	78	36	94	4.06e-15	65.9
MS.gene50653.t1	MTR_3g092220	43.836	73	38	2	15	85	28	99	4.81e-15	65.1
MS.gene50653.t1	MTR_1g094750	50.000	62	30	1	19	80	49	109	8.21e-15	64.3
MS.gene50653.t1	MTR_1g009150	50.820	61	29	1	20	80	71	130	1.70e-14	65.1
MS.gene50653.t1	MTR_8g461210	41.860	86	44	3	4	84	14	98	1.70e-14	63.5
MS.gene50653.t1	MTR_1g061570	44.118	68	37	1	19	86	18	84	1.97e-14	63.5
MS.gene50653.t1	MTR_4g025830	48.485	66	30	2	20	82	79	143	4.20e-14	63.5
MS.gene50653.t1	MTR_4g072160	46.053	76	36	3	8	80	23	96	6.34e-14	62.8
MS.gene50653.t1	MTR_1g059770	42.169	83	43	3	8	86	36	117	1.15e-13	61.6
MS.gene50653.t1	MTR_0388s0020	42.466	73	38	2	15	84	70	141	1.33e-13	62.0
MS.gene50653.t1	MTR_1g063950	43.077	65	36	1	19	83	47	110	3.65e-13	60.5
MS.gene50653.t1	MTR_4g005320	45.902	61	32	1	20	80	70	129	5.76e-13	60.8
MS.gene50653.t1	MTR_3g109160	46.269	67	35	1	20	86	69	134	8.48e-13	60.5
MS.gene50653.t1	MTR_8g101530	36.986	73	45	1	8	80	28	99	1.79e-12	58.9
MS.gene50653.t1	MTR_7g104940	43.548	62	34	1	23	84	53	113	2.15e-12	58.5
MS.gene50653.t1	MTR_7g101740	45.312	64	34	1	19	82	39	101	2.21e-12	58.2
MS.gene50653.t1	MTR_2g066830	39.759	83	48	2	2	84	41	121	2.95e-12	58.5
MS.gene50653.t1	MTR_7g104950	39.062	64	38	1	21	84	51	113	6.96e-12	57.4
MS.gene50653.t1	MTR_3g105900	69.444	36	11	0	19	54	1171	1206	9.73e-12	59.3
MS.gene50653.t1	MTR_3g097120	40.000	75	36	2	14	80	3	76	1.52e-11	55.8
MS.gene50653.t1	MTR_3g098970	35.526	76	48	1	12	87	45	119	1.73e-11	56.6
MS.gene50653.t1	MTR_2g095430	45.205	73	31	3	8	72	29	100	2.52e-11	55.8
MS.gene50653.t1	MTR_4g021810	46.774	62	31	2	20	80	26	86	2.88e-11	55.1
MS.gene50653.t1	MTR_1g063310	42.857	70	35	3	19	85	25	92	5.45e-11	54.3
MS.gene50653.t1	MTR_7g405740	45.763	59	31	1	22	80	69	126	8.02e-11	55.1
MS.gene50653.t1	MTR_8g101520	42.857	63	35	1	23	85	53	114	8.14e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50653.t1	AT4G38840	73.529	68	17	1	19	85	32	99	2.83e-32	108
MS.gene50653.t1	AT5G18080	76.923	65	14	1	22	85	26	90	5.38e-32	107
MS.gene50653.t1	AT5G18060	75.385	65	15	1	22	85	26	90	6.99e-32	107
MS.gene50653.t1	AT5G18020	77.419	62	13	1	22	82	26	87	9.67e-32	106
MS.gene50653.t1	AT5G18030	67.123	73	23	1	14	85	16	88	2.66e-31	105
MS.gene50653.t1	AT5G18050	73.846	65	16	1	22	85	26	90	2.75e-31	105
MS.gene50653.t1	AT3G03850	62.791	86	30	1	3	86	8	93	4.17e-31	105
MS.gene50653.t1	AT2G21200	67.123	73	23	1	13	84	14	86	5.23e-31	104
MS.gene50653.t1	AT3G03840	61.364	88	30	2	3	86	8	95	9.19e-31	104
MS.gene50653.t1	AT4G38825	65.753	73	24	1	14	85	17	89	2.89e-30	102
MS.gene50653.t1	AT5G18010	72.308	65	17	1	22	85	26	90	6.05e-30	102
MS.gene50653.t1	AT4G38850	64.474	76	24	1	10	82	11	86	8.35e-30	101
MS.gene50653.t1	AT4G34770	64.865	74	24	1	15	86	30	103	9.31e-30	102
MS.gene50653.t1	AT3G03820	61.905	84	27	2	8	86	13	96	1.88e-29	100
MS.gene50653.t1	AT2G21210	49.474	95	38	2	1	85	1	95	1.14e-28	99.0
MS.gene50653.t1	AT4G34800	62.195	82	25	3	1	77	1	81	3.10e-28	97.8
MS.gene50653.t1	AT2G21210	49.474	95	38	2	1	85	68	162	6.06e-28	99.4
MS.gene50653.t1	AT4G13790	57.143	77	31	1	8	82	13	89	1.64e-27	95.9
MS.gene50653.t1	AT4G34810	64.179	67	20	1	20	82	48	114	2.05e-27	96.7
MS.gene50653.t1	AT3G03830	57.831	83	31	2	3	82	8	89	8.18e-27	94.4
MS.gene50653.t1	AT4G34780	62.857	70	23	1	18	84	26	95	1.40e-25	91.7
MS.gene50653.t1	AT4G38860	57.353	68	29	0	18	85	38	105	6.53e-24	87.4
MS.gene50653.t1	AT4G34760	57.353	68	28	1	18	85	41	107	1.65e-23	86.3
MS.gene50653.t1	AT1G75580	61.194	67	25	1	18	84	42	107	2.74e-23	85.9
MS.gene50653.t1	AT2G21220	56.716	67	28	1	18	84	38	103	4.80e-23	85.1
MS.gene50653.t1	AT4G34790	65.000	60	19	1	20	77	39	98	1.54e-22	84.0
MS.gene50653.t1	AT2G16580	55.385	65	28	1	18	82	42	105	3.57e-20	77.8
MS.gene50653.t1	AT1G19830	51.429	70	33	1	15	84	43	111	8.76e-20	77.0
MS.gene50653.t1	AT4G36110	54.688	64	28	1	19	82	39	101	4.04e-19	75.1
MS.gene50653.t1	AT2G18010	53.125	64	29	1	19	82	47	109	2.83e-18	73.2
MS.gene50653.t1	AT3G43120	45.588	68	36	1	19	86	78	144	5.78e-17	71.2
MS.gene50653.t1	AT5G66260	47.368	76	39	1	10	85	24	98	1.02e-16	68.9
MS.gene50653.t1	AT3G20220	50.000	62	29	2	20	80	47	107	1.14e-16	69.3
MS.gene50653.t1	AT5G20810	48.485	66	33	1	19	84	78	142	3.61e-16	69.3
MS.gene50653.t1	AT5G20810	48.485	66	33	1	19	84	78	142	6.30e-16	68.9
MS.gene50653.t1	AT2G46690	45.977	87	39	4	3	86	11	92	1.48e-15	66.6
MS.gene50653.t1	AT1G75590	55.000	60	26	1	19	78	41	99	1.83e-15	67.0
MS.gene50653.t1	AT3G51200	49.123	57	28	1	21	77	41	96	2.52e-15	65.5
MS.gene50653.t1	AT5G10990	53.333	60	27	1	19	78	40	98	4.30e-15	65.9
MS.gene50653.t1	AT4G34750	45.312	64	34	1	19	82	36	98	4.94e-15	65.9
MS.gene50653.t1	AT4G34750	45.312	64	34	1	19	82	36	98	4.94e-15	65.9
MS.gene50653.t1	AT1G19840	55.000	60	26	1	19	78	40	98	1.66e-14	64.7
MS.gene50653.t1	AT3G61900	43.182	88	42	3	5	85	11	97	2.10e-14	63.9
MS.gene50653.t1	AT3G60690	43.836	73	37	2	13	82	81	152	9.06e-14	63.2
MS.gene50653.t1	AT2G37030	44.615	65	35	1	19	83	48	111	1.83e-13	61.2
MS.gene50653.t1	AT4G00880	47.826	69	33	2	19	85	27	94	5.00e-13	60.1
MS.gene50653.t1	AT4G31320	45.902	61	32	1	20	80	80	139	6.74e-13	61.2
MS.gene50653.t1	AT2G45210	42.857	70	36	2	16	82	75	143	7.36e-13	60.5
MS.gene50653.t1	AT3G09870	48.438	64	30	2	19	80	44	106	8.27e-13	59.3
MS.gene50653.t1	AT2G24400	47.541	61	31	1	20	80	64	123	1.86e-12	59.7
MS.gene50653.t1	AT5G53590	43.056	72	35	2	19	85	45	115	1.89e-12	58.9
MS.gene50653.t1	AT3G12830	38.372	86	48	2	4	85	30	114	4.78e-12	57.8
MS.gene50653.t1	AT4G09530	41.176	68	37	2	22	86	30	97	4.89e-12	57.0
MS.gene50653.t1	AT3G03847	43.038	79	37	2	8	80	54	130	5.05e-12	57.8
MS.gene50653.t1	AT1G56150	43.939	66	36	1	20	85	38	102	1.25e-11	56.2
MS.gene50653.t1	AT4G12410	46.875	64	30	2	20	80	76	138	2.49e-11	56.6
MS.gene50653.t1	AT3G53250	40.000	65	35	1	19	83	51	111	2.64e-11	55.8
MS.gene50653.t1	AT3G53250	40.000	65	35	1	19	83	82	142	3.27e-11	56.2
MS.gene50653.t1	AT2G36210	45.161	62	34	0	23	84	57	118	6.30e-11	55.1
MS.gene50653.t1	AT1G16510	44.615	65	35	1	20	84	54	117	7.05e-11	55.1
MS.gene50653.t1	AT4G22620	42.647	68	35	2	16	80	75	141	7.63e-11	55.1

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTAACTTCTTGCTTGAA+TGG	0.361989	4.3:-33300688	MS.gene50653:CDS
TCAAGCAAGAAGTTACGTTT+TGG	0.363874	4.3:+33300692	None:intergenic
CGTTTACATACTATTCTAAA+GGG	0.377068	4.3:-33300913	MS.gene50653:CDS
ATTTCAAGACTTGTTGAGTC+AGG	0.388693	4.3:-33300782	MS.gene50653:CDS
AGTCAGGCTGAGGAAGAGTT+TGG	0.389579	4.3:-33300766	MS.gene50653:CDS
CCGTTTACATACTATTCTAA+AGG	0.397138	4.3:-33300914	MS.gene50653:CDS
CTCAACAAGTCTTGAAATGA+AGG	0.426496	4.3:+33300787	None:intergenic
ACGTAACTTCTTGCTTGAAT+GGG	0.448506	4.3:-33300687	MS.gene50653:CDS
AAAATCTATAGAAGTGAGAA+AGG	0.452548	4.3:-33300878	MS.gene50653:CDS
CCTTTAGAATAGTATGTAAA+CGG	0.479197	4.3:+33300914	None:intergenic
ACAAGTCTTGAAATGAAGGT+TGG	0.516683	4.3:+33300791	None:intergenic
TGGAAGACATCTTCGGAGCA+TGG	0.525673	4.3:+33300712	None:intergenic
TTTGGATATGATCATCCAAT+GGG	0.545525	4.3:-33300748	MS.gene50653:CDS
AGACTTGTTGAGTCAGGCTG+AGG	0.545710	4.3:-33300776	MS.gene50653:CDS
GGCTATGTTGCAGTGTATGT+TGG	0.553556	4.3:-33300856	MS.gene50653:CDS
GACATCTTCGGAGCATGGAA+TGG	0.559799	4.3:+33300717	None:intergenic
GTTTGGATATGATCATCCAA+TGG	0.573812	4.3:-33300749	MS.gene50653:CDS
AAATCTATAGAAGTGAGAAA+GGG	0.580369	4.3:-33300877	MS.gene50653:CDS
GGAATGGTGAGGCCACCCAT+TGG	0.588290	4.3:+33300733	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.648071	4.3:-33300745	MS.gene50653:CDS
CTTCGGAGCATGGAATGGTG+AGG	0.671810	4.3:+33300722	None:intergenic
GGTTGGTTCAAGTATGACAC+AGG	0.680266	4.3:+33300808	None:intergenic
ATGTTGGAGAGAAACAGAAG+CGG	0.743921	4.3:-33300840	MS.gene50653:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATCTATAGAAGTGAGAA+AGG	-	chr4.3:33300725-33300744	MS.gene50653:CDS	25.0%
!	AAATCTATAGAAGTGAGAAA+GGG	-	chr4.3:33300726-33300745	MS.gene50653:CDS	25.0%
!	CGTTTACATACTATTCTAAA+GGG	-	chr4.3:33300690-33300709	MS.gene50653:CDS	25.0%
!!!	CCTTTAGAATAGTATGTAAA+CGG	+	chr4.3:33300692-33300711	None:intergenic	25.0%
	CCGTTTACATACTATTCTAA+AGG	-	chr4.3:33300689-33300708	MS.gene50653:CDS	30.0%
	TTTGGATATGATCATCCAAT+GGG	-	chr4.3:33300855-33300874	MS.gene50653:CDS	30.0%
	AACGTAACTTCTTGCTTGAA+TGG	-	chr4.3:33300915-33300934	MS.gene50653:CDS	35.0%
	ACAAGTCTTGAAATGAAGGT+TGG	+	chr4.3:33300815-33300834	None:intergenic	35.0%
	ACGTAACTTCTTGCTTGAAT+GGG	-	chr4.3:33300916-33300935	MS.gene50653:CDS	35.0%
	CTCAACAAGTCTTGAAATGA+AGG	+	chr4.3:33300819-33300838	None:intergenic	35.0%
	GTTTGGATATGATCATCCAA+TGG	-	chr4.3:33300854-33300873	MS.gene50653:CDS	35.0%
!	TCAAGCAAGAAGTTACGTTT+TGG	+	chr4.3:33300914-33300933	None:intergenic	35.0%
!!	ATTTCAAGACTTGTTGAGTC+AGG	-	chr4.3:33300821-33300840	MS.gene50653:CDS	35.0%
!!!	TACGTTTTGGAAGACATCTT+CGG	+	chr4.3:33300901-33300920	None:intergenic	35.0%
	ATGTTGGAGAGAAACAGAAG+CGG	-	chr4.3:33300763-33300782	MS.gene50653:CDS	40.0%
	GGATATGATCATCCAATGGG+TGG	-	chr4.3:33300858-33300877	MS.gene50653:CDS	45.0%
	GGCTATGTTGCAGTGTATGT+TGG	-	chr4.3:33300747-33300766	MS.gene50653:CDS	45.0%
!	GGTTGGTTCAAGTATGACAC+AGG	+	chr4.3:33300798-33300817	None:intergenic	45.0%
	AGTCAGGCTGAGGAAGAGTT+TGG	-	chr4.3:33300837-33300856	MS.gene50653:CDS	50.0%
	GACATCTTCGGAGCATGGAA+TGG	+	chr4.3:33300889-33300908	None:intergenic	50.0%
	TGGAAGACATCTTCGGAGCA+TGG	+	chr4.3:33300894-33300913	None:intergenic	50.0%
!!	AGACTTGTTGAGTCAGGCTG+AGG	-	chr4.3:33300827-33300846	MS.gene50653:CDS	50.0%
	CTTCGGAGCATGGAATGGTG+AGG	+	chr4.3:33300884-33300903	None:intergenic	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	+	chr4.3:33300873-33300892	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33300681	33300944	33300681	ID=MS.gene50653
chr4.3	mRNA	33300681	33300944	33300681	ID=MS.gene50653.t1;Parent=MS.gene50653
chr4.3	exon	33300681	33300944	33300681	ID=MS.gene50653.t1.exon1;Parent=MS.gene50653.t1
chr4.3	CDS	33300681	33300944	33300681	ID=cds.MS.gene50653.t1;Parent=MS.gene50653.t1

Gene Sequence	>MS.gene50653 ATGGGTTTCCGTTTACATACTATTCTAAAGGGTTCAGTAAAATCAAAATCTATAGAAGTGAGAAAGGGCTATGTTGCAGTGTATGTTGGAGAGAAACAGAAGCGGTTTGTAGTTCCTGTGTCATACTTGAACCAACCTTCATTTCAAGACTTGTTGAGTCAGGCTGAGGAAGAGTTTGGATATGATCATCCAATGGGTGGCCTCACCATTCCATGCTCCGAAGATGTCTTCCAAAACGTAACTTCTTGCTTGAATGGGATATGA
Protein sequence	>MS.gene50653.t1 MGFRLHTILKGSVKSKSIEVRKGYVAVYVGEKQKRFVVPVSYLNQPSFQDLLSQAEEEFGYDHPMGGLTIPCSEDVFQNVTSCLNGI