AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57175


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57175.t1 MTR_5g016450 86.111 180 21 2 1 179 1 177 5.18e-110 311
MS.gene57175.t1 MTR_5g016450 85.635 181 21 3 1 179 1 178 3.17e-108 306
MS.gene57175.t1 MTR_5g016450 85.629 167 20 2 1 166 1 164 7.01e-101 288
MS.gene57175.t1 MTR_3g462220 41.279 172 84 7 9 176 6 164 2.05e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57175.t1 AT4G37300 46.111 180 85 4 5 179 1 173 3.00e-44 144

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGCTTCTGTTGGGTTTGA+TGG 0.233826 5.3:+9188020 None:intergenic
TTTGATTTGGTTAGTGATCT+TGG 0.257781 5.3:+9188074 None:intergenic
CATCGAATTGAGCTTTGATT+TGG 0.326561 5.3:+9188061 None:intergenic
CTATGAAAATAGACAGGAAA+TGG 0.362452 5.3:-9187727 MS.gene57175:intron
TAGGTGCTCTTGCTCCTATT+CGG 0.371782 5.3:+9187372 None:intergenic
CCAACATCAACATGGCTTGC+AGG 0.374328 5.3:+9187652 None:intergenic
GTTGGTGAAAACAAATTTAG+AGG 0.379408 5.3:-9187634 MS.gene57175:CDS
TGTTTCAGGTTCGCTTCTGT+TGG 0.393664 5.3:+9188011 None:intergenic
GCAACCCTGCCACAAATGAT+TGG 0.403822 5.3:-9187603 MS.gene57175:CDS
TCAACACAACATACCAGAAC+TGG 0.405279 5.3:-9187959 MS.gene57175:CDS
AGTGATCTTGGTTTGTTCCT+CGG 0.406528 5.3:+9188086 None:intergenic
TTGGGTTTGATGGGTCGTTT+TGG 0.407967 5.3:+9188030 None:intergenic
TGTATCTACTAATCCCTTTG+AGG 0.412491 5.3:+9187894 None:intergenic
TAACCTTGATCATGAACATC+TGG 0.442683 5.3:-9187575 MS.gene57175:intron
TCGCTTCTGTTGGGTTTGAT+GGG 0.451444 5.3:+9188021 None:intergenic
TATCAATTGAAAACAACTCT+TGG 0.451803 5.3:+9187842 None:intergenic
GGAACCCAATCATTTGTGGC+AGG 0.462082 5.3:+9187598 None:intergenic
ATGTCTCGTCTAAGCCGAAT+AGG 0.462907 5.3:-9187386 MS.gene57175:CDS
CAGACTATACTTTCCTCAAA+GGG 0.469094 5.3:-9187907 MS.gene57175:CDS
GTTTCAGGTTCGCTTCTGTT+GGG 0.489259 5.3:+9188012 None:intergenic
GTTAGAGAAGGAGATGAAAG+TGG 0.492647 5.3:-9187691 MS.gene57175:CDS
CAACCCTGCCACAAATGATT+GGG 0.493231 5.3:-9187602 MS.gene57175:CDS
AGATGTTCATGATCAAGGTT+AGG 0.495165 5.3:+9187577 None:intergenic
TTTATAAAAGCGGTTAGAGA+AGG 0.500477 5.3:-9187703 MS.gene57175:CDS
GTTAGGAACCCAATCATTTG+TGG 0.507173 5.3:+9187594 None:intergenic
AGATACACAAGATGAGTTTG+TGG 0.508234 5.3:-9187878 MS.gene57175:CDS
ATACCAGATGTTCATGATCA+AGG 0.509861 5.3:+9187572 None:intergenic
TATGAAAATAGACAGGAAAT+GGG 0.515295 5.3:-9187726 MS.gene57175:intron
AAAACCTCGTCACTACGACT+TGG 0.560143 5.3:+9188114 None:intergenic
TCAGACTATACTTTCCTCAA+AGG 0.574625 5.3:-9187908 MS.gene57175:CDS
CCTGCAAGCCATGTTGATGT+TGG 0.587170 5.3:-9187652 MS.gene57175:CDS
AGGAAATGGGTTTATAAAAG+CGG 0.590246 5.3:-9187713 MS.gene57175:CDS
GAACCCAATCATTTGTGGCA+GGG 0.594079 5.3:+9187599 None:intergenic
CCAAACAACAACATCATGGT+AGG 0.619313 5.3:-9188156 None:intergenic
AATTGATAAACAGCATCACA+CGG 0.634825 5.3:-9187827 MS.gene57175:intron
AAAACCAAGTCGTAGTGACG+AGG 0.696472 5.3:-9188118 MS.gene57175:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTGTTTCTATATTTATTTA+TGG - chr5.3:9187721-9187740 MS.gene57175:intron 15.0%
!!! TTTTTGTGATTATGTTTTTT+TGG - chr5.3:9187755-9187774 MS.gene57175:intron 15.0%
! ATTGAACTATGAAAATAGAC+AGG - chr5.3:9187779-9187798 MS.gene57175:intron 25.0%
! TATCAATTGAAAACAACTCT+TGG + chr5.3:9187673-9187692 None:intergenic 25.0%
! TATGAAAATAGACAGGAAAT+GGG - chr5.3:9187786-9187805 MS.gene57175:intron 25.0%
!!! ATAGATTGTGATTTGTTAAC+AGG - chr5.3:9188100-9188119 MS.gene57175:CDS 25.0%
AATTGATAAACAGCATCACA+CGG - chr5.3:9187685-9187704 MS.gene57175:CDS 30.0%
CTATGAAAATAGACAGGAAA+TGG - chr5.3:9187785-9187804 MS.gene57175:intron 30.0%
TATGTTGTGTTGAGAAAAAG+TGG + chr5.3:9187546-9187565 None:intergenic 30.0%
TTTATAAAAGCGGTTAGAGA+AGG - chr5.3:9187809-9187828 MS.gene57175:intron 30.0%
! AGGAAATGGGTTTATAAAAG+CGG - chr5.3:9187799-9187818 MS.gene57175:intron 30.0%
! GTTGGTGAAAACAAATTTAG+AGG - chr5.3:9187878-9187897 MS.gene57175:CDS 30.0%
!! TTTGATTTGGTTAGTGATCT+TGG + chr5.3:9187441-9187460 None:intergenic 30.0%
AGATACACAAGATGAGTTTG+TGG - chr5.3:9187634-9187653 MS.gene57175:CDS 35.0%
AGATGTTCATGATCAAGGTT+AGG + chr5.3:9187938-9187957 None:intergenic 35.0%
ATACCAGATGTTCATGATCA+AGG + chr5.3:9187943-9187962 None:intergenic 35.0%
CAGACTATACTTTCCTCAAA+GGG - chr5.3:9187605-9187624 MS.gene57175:CDS 35.0%
GTCAAAGTTCTTTACACGAT+CGG - chr5.3:9188046-9188065 MS.gene57175:CDS 35.0%
TAACCTTGATCATGAACATC+TGG - chr5.3:9187937-9187956 MS.gene57175:CDS 35.0%
TCAGACTATACTTTCCTCAA+AGG - chr5.3:9187604-9187623 MS.gene57175:CDS 35.0%
TGTATCTACTAATCCCTTTG+AGG + chr5.3:9187621-9187640 None:intergenic 35.0%
TTGAGAAAAAGTGGAGTCAA+CGG + chr5.3:9187537-9187556 None:intergenic 35.0%
! CATCGAATTGAGCTTTGATT+TGG + chr5.3:9187454-9187473 None:intergenic 35.0%
! TGTTTTCACCAACATCAACA+TGG + chr5.3:9187871-9187890 None:intergenic 35.0%
!! GTTTGAAGTTTTTCCAGTTC+TGG + chr5.3:9187569-9187588 None:intergenic 35.0%
GTTAGAGAAGGAGATGAAAG+TGG - chr5.3:9187821-9187840 MS.gene57175:intron 40.0%
GTTAGGAACCCAATCATTTG+TGG + chr5.3:9187921-9187940 None:intergenic 40.0%
TCAACACAACATACCAGAAC+TGG - chr5.3:9187553-9187572 MS.gene57175:intron 40.0%
TGAGAAAAAGTGGAGTCAAC+GGG + chr5.3:9187536-9187555 None:intergenic 40.0%
!! AGTGATCTTGGTTTGTTCCT+CGG + chr5.3:9187429-9187448 None:intergenic 40.0%
AAAACCAAGTCGTAGTGACG+AGG - chr5.3:9187394-9187413 MS.gene57175:intron 45.0%
AAAACCTCGTCACTACGACT+TGG + chr5.3:9187401-9187420 None:intergenic 45.0%
AAACAGCATCACACGGTTTG+TGG - chr5.3:9187692-9187711 MS.gene57175:CDS 45.0%
ATGTCTCGTCTAAGCCGAAT+AGG - chr5.3:9188126-9188145 MS.gene57175:CDS 45.0%
CAACCCTGCCACAAATGATT+GGG - chr5.3:9187910-9187929 MS.gene57175:CDS 45.0%
GAACCCAATCATTTGTGGCA+GGG + chr5.3:9187916-9187935 None:intergenic 45.0%
GAGAAAAAGTGGAGTCAACG+GGG + chr5.3:9187535-9187554 None:intergenic 45.0%
TAGGTGCTCTTGCTCCTATT+CGG + chr5.3:9188143-9188162 None:intergenic 45.0%
TCGCTTCTGTTGGGTTTGAT+GGG + chr5.3:9187494-9187513 None:intergenic 45.0%
TTCGCTTCTGTTGGGTTTGA+TGG + chr5.3:9187495-9187514 None:intergenic 45.0%
!! GTTTCAGGTTCGCTTCTGTT+GGG + chr5.3:9187503-9187522 None:intergenic 45.0%
!! TGTTTCAGGTTCGCTTCTGT+TGG + chr5.3:9187504-9187523 None:intergenic 45.0%
!! TTGGGTTTGATGGGTCGTTT+TGG + chr5.3:9187485-9187504 None:intergenic 45.0%
!!! ACGGGGTTTTGTTGTGTTTC+AGG + chr5.3:9187518-9187537 None:intergenic 45.0%
CCTGCAAGCCATGTTGATGT+TGG - chr5.3:9187860-9187879 MS.gene57175:CDS 50.0%
GCAACCCTGCCACAAATGAT+TGG - chr5.3:9187909-9187928 MS.gene57175:CDS 50.0%
GGAACCCAATCATTTGTGGC+AGG + chr5.3:9187917-9187936 None:intergenic 50.0%
! CCAACATCAACATGGCTTGC+AGG + chr5.3:9187863-9187882 None:intergenic 50.0%
!!! TGACGAGGTTTTGAACACCG+AGG - chr5.3:9187409-9187428 MS.gene57175:intron 50.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 9187371 9188163 9187371 ID=MS.gene57175
chr5.3 mRNA 9187371 9188163 9187371 ID=MS.gene57175.t1;Parent=MS.gene57175
chr5.3 exon 9187828 9188163 9187828 ID=MS.gene57175.t1.exon1;Parent=MS.gene57175.t1
chr5.3 CDS 9187828 9188163 9187828 ID=cds.MS.gene57175.t1;Parent=MS.gene57175.t1
chr5.3 exon 9187576 9187737 9187576 ID=MS.gene57175.t1.exon2;Parent=MS.gene57175.t1
chr5.3 CDS 9187576 9187737 9187576 ID=cds.MS.gene57175.t1;Parent=MS.gene57175.t1
chr5.3 exon 9187371 9187412 9187371 ID=MS.gene57175.t1.exon3;Parent=MS.gene57175.t1
chr5.3 CDS 9187371 9187412 9187371 ID=cds.MS.gene57175.t1;Parent=MS.gene57175.t1
Gene Sequence

>MS.gene57175

ATGGTAGGACAGTTGACAACTACAAAACCAAGTCGTAGTGACGAGGTTTTGAACACCGAGGAACAAACCAAGATCACTAACCAAATCAAAGCTCAATTCGATGCTTTAACACCAAAACGACCCATCAAACCCAACAGAAGCGAACCTGAAACACAACAAAACCCCGTTGACTCCACTTTTTCTCAACACAACATACCAGAACTGGAAAAACTTCAAACTCTTCAATCAAATTCTCAGACTATACTTTCCTCAAAGGGATTAGTAGATACACAAGATGAGTTTGTGGAAACAAAGTATTACCAAGAGTTGTTTTCAATTGATAAACAGCATCACACGGTTTGTGGTTTTACTCTGTTTCTATATTTATTTATGGTTTTTTTTTTTTTTTTGTGATTATGTTTTTTTGGTATTGAACTATGAAAATAGACAGGAAATGGGTTTATAAAAGCGGTTAGAGAAGGAGATGAAAGTGGATATGAGATTGAGTTACCTGCAAGCCATGTTGATGTTGGTGAAAACAAATTTAGAGGCTATAAAAGCAACCCTGCCACAAATGATTGGGTTCCTAACCTTGATCATGAACATCTGGTATATGATATATTCATTTAGAGAGAGATATCTTATTTATTATTGATAGATCAAATTCTAAGAATTTAATTCATATACATTATGAGTGTCAAAGTTCTTTACACGATCGGATATATAACGAAATCAAAACATACATATATGATAGATTGTGATTTGTTAACAGGACTATGTCTCGTCTAAGCCGAATAGGAGCAAGAGCACCTAG

Protein sequence

>MS.gene57175.t1

MVGQLTTTKPSRSDEVLNTEEQTKITNQIKAQFDALTPKRPIKPNRSEPETQQNPVDSTFSQHNIPELEKLQTLQSNSQTILSSKGLVDTQDEFVETKYYQELFSIDKQHHTTGNGFIKAVREGDESGYEIELPASHVDVGENKFRGYKSNPATNDWVPNLDHEHLDYVSSKPNRSKST