Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69692.t1 | XP_013446022.1 | 93.2 | 88 | 6 | 0 | 4 | 91 | 1 | 88 | 2.50E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69692.t1 | A0A392NV46 | 90.2 | 92 | 9 | 0 | 1 | 92 | 3 | 94 | 3.7e-40 | 173.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene69692 | 0.816153 | 6.40E-52 | -1.69E-46 |
| MS.gene04920 | MS.gene69692 | 0.804031 | 2.63E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene69692 | 0.803772 | 2.97E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene69692 | 0.86213 | 6.30E-64 | -1.69E-46 |
| MS.gene049368 | MS.gene69692 | 0.804256 | 2.36E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene69692 | -0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene69692 | 0.820516 | 6.58E-53 | -1.69E-46 |
| MS.gene049786 | MS.gene69692 | 0.807258 | 5.52E-50 | -1.69E-46 |
| MS.gene049801 | MS.gene69692 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene69692 | 0.865209 | 6.97E-65 | -1.69E-46 |
| MS.gene04998 | MS.gene69692 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene69692 | 0.802112 | 6.55E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene69692 | 0.825717 | 4.02E-54 | -1.69E-46 |
| MS.gene050414 | MS.gene69692 | 0.819417 | 1.17E-52 | -1.69E-46 |
| MS.gene050571 | MS.gene69692 | 0.831652 | 1.48E-55 | -1.69E-46 |
| MS.gene050646 | MS.gene69692 | 0.841407 | 4.84E-58 | -1.69E-46 |
| MS.gene050654 | MS.gene69692 | 0.875513 | 2.90E-68 | -1.69E-46 |
| MS.gene050941 | MS.gene69692 | 0.831993 | 1.22E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene69692 | 0.816647 | 4.96E-52 | -1.69E-46 |
| MS.gene051122 | MS.gene69692 | 0.822468 | 2.33E-53 | -1.69E-46 |
| MS.gene051184 | MS.gene69692 | 0.839442 | 1.58E-57 | -1.69E-46 |
| MS.gene051343 | MS.gene69692 | 0.822409 | 2.41E-53 | -1.69E-46 |
| MS.gene051490 | MS.gene69692 | 0.807934 | 3.97E-50 | -1.69E-46 |
| MS.gene051541 | MS.gene69692 | 0.816743 | 4.73E-52 | -1.69E-46 |
| MS.gene051800 | MS.gene69692 | 0.804896 | 1.73E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene69692 | 0.834116 | 3.61E-56 | -1.69E-46 |
| MS.gene051852 | MS.gene69692 | 0.812896 | 3.37E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene69692 | 0.833879 | 4.14E-56 | -1.69E-46 |
| MS.gene052118 | MS.gene69692 | 0.818902 | 1.54E-52 | -1.69E-46 |
| MS.gene052242 | MS.gene69692 | -0.80645 | 8.18E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene69692 | 0.830833 | 2.35E-55 | -1.69E-46 |
| MS.gene052342 | MS.gene69692 | 0.812443 | 4.23E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene69692 | 0.831961 | 1.24E-55 | -1.69E-46 |
| MS.gene052463 | MS.gene69692 | 0.826078 | 3.30E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene69692 | 0.834141 | 3.56E-56 | -1.69E-46 |
| MS.gene052674 | MS.gene69692 | 0.830722 | 2.50E-55 | -1.69E-46 |
| MS.gene052675 | MS.gene69692 | 0.823995 | 1.03E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene69692 | 0.824048 | 9.97E-54 | -1.69E-46 |
| MS.gene052776 | MS.gene69692 | 0.801331 | 9.47E-49 | -1.69E-46 |
| MS.gene052855 | MS.gene69692 | 0.80255 | 5.32E-49 | -1.69E-46 |
| MS.gene052932 | MS.gene69692 | 0.815448 | 9.20E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene69692 | 0.823044 | 1.71E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene69692 | 0.819275 | 1.26E-52 | -1.69E-46 |
| MS.gene053298 | MS.gene69692 | 0.820523 | 6.56E-53 | -1.69E-46 |
| MS.gene053456 | MS.gene69692 | 0.844375 | 7.86E-59 | -1.69E-46 |
| MS.gene053575 | MS.gene69692 | 0.81232 | 4.50E-51 | -1.69E-46 |
| MS.gene053726 | MS.gene69692 | 0.835815 | 1.35E-56 | -1.69E-46 |
| MS.gene053963 | MS.gene69692 | 0.817818 | 2.71E-52 | -1.69E-46 |
| MS.gene05439 | MS.gene69692 | 0.818144 | 2.28E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene69692 | 0.852327 | 4.97E-61 | -1.69E-46 |
| MS.gene05458 | MS.gene69692 | 0.803127 | 4.04E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene69692 | 0.861047 | 1.35E-63 | -1.69E-46 |
| MS.gene054893 | MS.gene69692 | 0.865929 | 4.13E-65 | -1.69E-46 |
| MS.gene054957 | MS.gene69692 | 0.800159 | 1.64E-48 | -1.69E-46 |
| MS.gene055147 | MS.gene69692 | 0.814473 | 1.51E-51 | -1.69E-46 |
| MS.gene055222 | MS.gene69692 | 0.806328 | 8.68E-50 | -1.69E-46 |
| MS.gene055226 | MS.gene69692 | 0.800205 | 1.61E-48 | -1.69E-46 |
| MS.gene055311 | MS.gene69692 | 0.827478 | 1.53E-54 | -1.69E-46 |
| MS.gene055313 | MS.gene69692 | 0.826282 | 2.95E-54 | -1.69E-46 |
| MS.gene055314 | MS.gene69692 | 0.800517 | 1.39E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene69692 | 0.847423 | 1.17E-59 | -1.69E-46 |
| MS.gene055724 | MS.gene69692 | 0.802481 | 5.50E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene69692 | 0.836839 | 7.39E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene69692 | 0.801651 | 8.14E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene69692 | 0.862391 | 5.24E-64 | -1.69E-46 |
| MS.gene056097 | MS.gene69692 | 0.825414 | 4.75E-54 | -1.69E-46 |
| MS.gene05615 | MS.gene69692 | 0.814713 | 1.34E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene69692 | 0.857328 | 1.76E-62 | -1.69E-46 |
| MS.gene056699 | MS.gene69692 | 0.84286 | 2.00E-58 | -1.69E-46 |
| MS.gene056787 | MS.gene69692 | 0.813917 | 2.01E-51 | -1.69E-46 |
| MS.gene056831 | MS.gene69692 | 0.841723 | 4.00E-58 | -1.69E-46 |
| MS.gene056849 | MS.gene69692 | 0.828181 | 1.04E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene69692 | 0.81907 | 1.41E-52 | -1.69E-46 |
| MS.gene056973 | MS.gene69692 | 0.823595 | 1.27E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene69692 | 0.808002 | 3.84E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene69692 | 0.806649 | 7.42E-50 | -1.69E-46 |
| MS.gene057034 | MS.gene69692 | 0.819989 | 8.69E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene69692 | 0.800978 | 1.12E-48 | -1.69E-46 |
| MS.gene057155 | MS.gene69692 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene69692 | 0.812057 | 5.13E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene69692 | 0.804931 | 1.70E-49 | -1.69E-46 |
| MS.gene057919 | MS.gene69692 | 0.801935 | 7.12E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene69692 | 0.803556 | 3.29E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene69692 | 0.802194 | 6.30E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene69692 | 0.805677 | 1.19E-49 | -1.69E-46 |
| MS.gene058095 | MS.gene69692 | 0.801055 | 1.08E-48 | -1.69E-46 |
| MS.gene058159 | MS.gene69692 | 0.811187 | 7.94E-51 | -1.69E-46 |
| MS.gene058223 | MS.gene69692 | -0.808133 | 3.60E-50 | -1.69E-46 |
| MS.gene058278 | MS.gene69692 | 0.804691 | 1.91E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene69692 | 0.827838 | 1.25E-54 | -1.69E-46 |
| MS.gene058292 | MS.gene69692 | 0.816108 | 6.55E-52 | -1.69E-46 |
| MS.gene058429 | MS.gene69692 | 0.853036 | 3.12E-61 | -1.69E-46 |
| MS.gene058592 | MS.gene69692 | 0.825443 | 4.67E-54 | -1.69E-46 |
| MS.gene058758 | MS.gene69692 | 0.811865 | 5.65E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene69692 | 0.800212 | 1.60E-48 | -1.69E-46 |
| MS.gene059007 | MS.gene69692 | 0.807284 | 5.45E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene69692 | 0.826397 | 2.77E-54 | -1.69E-46 |
| MS.gene059170 | MS.gene69692 | 0.826306 | 2.92E-54 | -1.69E-46 |
| MS.gene059283 | MS.gene69692 | 0.80692 | 6.51E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene69692 | 0.830867 | 2.31E-55 | -1.69E-46 |
| MS.gene059965 | MS.gene69692 | 0.831485 | 1.63E-55 | -1.69E-46 |
| MS.gene06010 | MS.gene69692 | 0.811444 | 6.98E-51 | -1.69E-46 |
| MS.gene060259 | MS.gene69692 | 0.840859 | 6.75E-58 | -1.69E-46 |
| MS.gene060412 | MS.gene69692 | 0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene060520 | MS.gene69692 | 0.803483 | 3.41E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene69692 | 0.808001 | 3.84E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene69692 | 0.839665 | 1.38E-57 | -1.69E-46 |
| MS.gene060840 | MS.gene69692 | 0.821317 | 4.31E-53 | -1.69E-46 |
| MS.gene060850 | MS.gene69692 | 0.827324 | 1.67E-54 | -1.69E-46 |
| MS.gene060851 | MS.gene69692 | 0.83104 | 2.09E-55 | -1.69E-46 |
| MS.gene060852 | MS.gene69692 | 0.832081 | 1.16E-55 | -1.69E-46 |
| MS.gene060932 | MS.gene69692 | 0.865141 | 7.32E-65 | -1.69E-46 |
| MS.gene060933 | MS.gene69692 | 0.858391 | 8.51E-63 | -1.69E-46 |
| MS.gene060935 | MS.gene69692 | 0.833404 | 5.44E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene69692 | 0.850144 | 2.05E-60 | -1.69E-46 |
| MS.gene061100 | MS.gene69692 | 0.822787 | 1.97E-53 | -1.69E-46 |
| MS.gene06121 | MS.gene69692 | 0.832267 | 1.04E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69692.t1 | MTR_8g065050 | 93.182 | 88 | 6 | 0 | 4 | 91 | 1 | 88 | 9.37e-57 | 174 |
| MS.gene69692.t1 | MTR_1g084740 | 55.914 | 93 | 37 | 3 | 4 | 92 | 1 | 93 | 2.65e-29 | 103 |
| MS.gene69692.t1 | MTR_1g084740 | 56.522 | 92 | 36 | 3 | 4 | 91 | 1 | 92 | 1.72e-28 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69692.t1 | AT1G23440 | 64.045 | 89 | 32 | 0 | 4 | 92 | 1 | 89 | 3.96e-37 | 120 |
| MS.gene69692.t1 | AT1G23440 | 62.500 | 88 | 33 | 0 | 4 | 91 | 1 | 88 | 9.95e-35 | 118 |
| MS.gene69692.t1 | AT1G23440 | 62.500 | 88 | 33 | 0 | 4 | 91 | 1 | 88 | 9.95e-35 | 118 |
| MS.gene69692.t1 | AT1G56700 | 52.809 | 89 | 41 | 1 | 4 | 91 | 1 | 89 | 7.33e-28 | 100 |
| MS.gene69692.t1 | AT1G56700 | 52.809 | 89 | 41 | 1 | 4 | 91 | 1 | 89 | 7.33e-28 | 100 |
| MS.gene69692.t1 | AT1G56700 | 52.809 | 89 | 41 | 1 | 4 | 91 | 1 | 89 | 7.33e-28 | 100 |
| MS.gene69692.t1 | AT1G56700 | 43.333 | 60 | 33 | 1 | 33 | 91 | 1 | 60 | 3.39e-11 | 57.0 |
Find 25 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATCTCTGTAGGGTTTAT+TGG | 0.189118 | 8.3:-36986924 | None:intergenic |
| AAAATTACAATTCATGTAAC+TGG | 0.219675 | 8.3:+36986879 | MS.gene69692:CDS |
| AGAGTAATGCAAATGTTGTT+TGG | 0.313563 | 8.3:+36987096 | MS.gene69692:CDS |
| CTTCCAGTTGGTGTTACTCT+TGG | 0.368291 | 8.3:+36986984 | MS.gene69692:CDS |
| AAGAATTCAGAATTCAGAAA+TGG | 0.372313 | 8.3:+36986839 | None:intergenic |
| GAGTAATGCAAATGTTGTTT+GGG | 0.392945 | 8.3:+36987097 | MS.gene69692:CDS |
| CTTCCAAGAGTAACACCAAC+TGG | 0.451339 | 8.3:-36986987 | None:intergenic |
| ACTGGGTTTAAGAAGTTTCA+AGG | 0.460584 | 8.3:+36986897 | MS.gene69692:CDS |
| TTATTAACAATAATCTCTGT+AGG | 0.465678 | 8.3:-36986933 | None:intergenic |
| AGAATTCAGAATTCAGAAAT+GGG | 0.477928 | 8.3:+36986840 | None:intergenic |
| TGCACTATTCTTGACGTGGC+TGG | 0.492051 | 8.3:+36987011 | MS.gene69692:CDS |
| ATTCTTGACGTGGCTGGTGA+TGG | 0.492649 | 8.3:+36987017 | MS.gene69692:CDS |
| AGGGATTATGTTGAAAAGAA+AGG | 0.499009 | 8.3:+36986960 | MS.gene69692:CDS |
| GGAATCTGTTGTGTCGAAAA+CGG | 0.508223 | 8.3:+36987067 | MS.gene69692:CDS |
| TTTCGACACAACAGATTCCA+TGG | 0.522218 | 8.3:-36987063 | None:intergenic |
| AATTCAGAAATGGGATCTGA+AGG | 0.532059 | 8.3:+36986849 | MS.gene69692:CDS |
| AGATTATTGTTAATAATCTG+AGG | 0.547702 | 8.3:+36986940 | MS.gene69692:CDS |
| TATTAACAATAATCTCTGTA+GGG | 0.553359 | 8.3:-36986932 | None:intergenic |
| AAATTACAATTCATGTAACT+GGG | 0.560230 | 8.3:+36986880 | MS.gene69692:CDS |
| TCCTCAGCTTTATCAGACCA+TGG | 0.578488 | 8.3:+36987046 | MS.gene69692:CDS |
| GATTATTGTTAATAATCTGA+GGG | 0.580790 | 8.3:+36986941 | MS.gene69692:CDS |
| GAAAAGAAAGGTCTTCCAGT+TGG | 0.590107 | 8.3:+36986972 | MS.gene69692:CDS |
| ATTCAGAAATGGGATCTGAA+GGG | 0.630200 | 8.3:+36986850 | MS.gene69692:CDS |
| AAGTTGCACTATTCTTGACG+TGG | 0.643245 | 8.3:+36987007 | MS.gene69692:CDS |
| TCCATGGTCTGATAAAGCTG+AGG | 0.663058 | 8.3:-36987047 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTACAATTCATGTAAC+TGG | + | chr8.3:36986879-36986898 | MS.gene69692:CDS | 20.0% |
| !! | AAATTACAATTCATGTAACT+GGG | + | chr8.3:36986880-36986899 | MS.gene69692:CDS | 20.0% |
| !! | AGATTATTGTTAATAATCTG+AGG | + | chr8.3:36986940-36986959 | MS.gene69692:CDS | 20.0% |
| !! | GATTATTGTTAATAATCTGA+GGG | + | chr8.3:36986941-36986960 | MS.gene69692:CDS | 20.0% |
| !! | TATTAACAATAATCTCTGTA+GGG | - | chr8.3:36986935-36986954 | None:intergenic | 20.0% |
| !! | TTATTAACAATAATCTCTGT+AGG | - | chr8.3:36986936-36986955 | None:intergenic | 20.0% |
| !!! | ACATGAATTGTAATTTTCTT+TGG | - | chr8.3:36986876-36986895 | None:intergenic | 20.0% |
| AGAGTAATGCAAATGTTGTT+TGG | + | chr8.3:36987096-36987115 | MS.gene69692:CDS | 30.0% | |
| AGGGATTATGTTGAAAAGAA+AGG | + | chr8.3:36986960-36986979 | MS.gene69692:CDS | 30.0% | |
| ATAATCTCTGTAGGGTTTAT+TGG | - | chr8.3:36986927-36986946 | None:intergenic | 30.0% | |
| ! | GAGTAATGCAAATGTTGTTT+GGG | + | chr8.3:36987097-36987116 | MS.gene69692:CDS | 30.0% |
| AATTCAGAAATGGGATCTGA+AGG | + | chr8.3:36986849-36986868 | MS.gene69692:CDS | 35.0% | |
| ACTGGGTTTAAGAAGTTTCA+AGG | + | chr8.3:36986897-36986916 | MS.gene69692:CDS | 35.0% | |
| ATTCAGAAATGGGATCTGAA+GGG | + | chr8.3:36986850-36986869 | MS.gene69692:CDS | 35.0% | |
| AAGTTGCACTATTCTTGACG+TGG | + | chr8.3:36987007-36987026 | MS.gene69692:CDS | 40.0% | |
| GAAAAGAAAGGTCTTCCAGT+TGG | + | chr8.3:36986972-36986991 | MS.gene69692:CDS | 40.0% | |
| GGAATCTGTTGTGTCGAAAA+CGG | + | chr8.3:36987067-36987086 | MS.gene69692:CDS | 40.0% | |
| TTTCGACACAACAGATTCCA+TGG | - | chr8.3:36987066-36987085 | None:intergenic | 40.0% | |
| CTTCCAAGAGTAACACCAAC+TGG | - | chr8.3:36986990-36987009 | None:intergenic | 45.0% | |
| TCCATGGTCTGATAAAGCTG+AGG | - | chr8.3:36987050-36987069 | None:intergenic | 45.0% | |
| ! | TCCTCAGCTTTATCAGACCA+TGG | + | chr8.3:36987046-36987065 | MS.gene69692:CDS | 45.0% |
| !! | CTTCCAGTTGGTGTTACTCT+TGG | + | chr8.3:36986984-36987003 | MS.gene69692:CDS | 45.0% |
| ATTCTTGACGTGGCTGGTGA+TGG | + | chr8.3:36987017-36987036 | MS.gene69692:CDS | 50.0% | |
| TGCACTATTCTTGACGTGGC+TGG | + | chr8.3:36987011-36987030 | MS.gene69692:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 36986849 | 36987127 | 36986849 | ID=MS.gene69692 |
| chr8.3 | mRNA | 36986849 | 36987127 | 36986849 | ID=MS.gene69692.t1;Parent=MS.gene69692 |
| chr8.3 | exon | 36986849 | 36987127 | 36986849 | ID=MS.gene69692.t1.exon1;Parent=MS.gene69692.t1 |
| chr8.3 | CDS | 36986849 | 36987127 | 36986849 | ID=cds.MS.gene69692.t1;Parent=MS.gene69692.t1 |
| Gene Sequence |
| Protein sequence |
