AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69810.t1 MTR_2g082930 97.674 215 5 0 1 215 1 215 1.67e-157 434
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69810.t1 AT1G51100 68.449 187 47 4 38 215 28 211 1.60e-81 242

Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTATTGGAAATGTTTC+TGG 0.086996 2.4:+16459129 None:intergenic
TTCCAACCTATTTCAAAATT+TGG 0.094353 2.4:+16459042 None:intergenic
GGGTCTGGAGTTGGAAATTC+TGG 0.299445 2.4:+16459174 None:intergenic
CTGTTCATTGTAGTGTTTGT+AGG 0.309624 2.4:+16459153 None:intergenic
ATTGTAGTGTTTGTAGGGTC+TGG 0.310567 2.4:+16459159 None:intergenic
TCAGAAGATGTGGAATTTAA+TGG 0.324162 2.4:+16459198 None:intergenic
TGAAAGTTGTGAAGATGTTT+TGG 0.348563 2.4:+16459269 None:intergenic
GAGGTTGATGGAAATGGATT+TGG 0.355148 2.4:-16458803 MS.gene69810:CDS
GAACTTATGAAGAAGGAATA+TGG 0.383069 2.4:-16458949 MS.gene69810:CDS
TGTTCATTGTAGTGTTTGTA+GGG 0.442377 2.4:+16459154 None:intergenic
TTGTGTAGATGATGAGGTGC+AGG 0.469618 2.4:+16459303 None:intergenic
GTTGGAGGAACTTATGAAGA+AGG 0.476939 2.4:-16458956 MS.gene69810:CDS
TGGCTCGGCATTTGCAAGGT+CGG 0.477066 2.4:+16459065 None:intergenic
CAACCTATTTCAAAATTTGG+TGG 0.478009 2.4:+16459045 None:intergenic
AGGAAAAGTTGAGGAGAGAA+TGG 0.490855 2.4:-16458848 MS.gene69810:CDS
ATGAAGAGTTAAAATTCAAA+AGG 0.492536 2.4:+16458687 None:intergenic
GAGAGATGAAGGAAAAGTTG+AGG 0.497620 2.4:-16458857 MS.gene69810:CDS
ACAGAACTGTTGGCAAAAGT+TGG 0.501386 2.4:-16458922 MS.gene69810:CDS
TATTTCAAAATTTGGTGGCT+CGG 0.505623 2.4:+16459050 None:intergenic
TATGGTGAAAGCTTCTGTTA+AGG 0.539994 2.4:-16458779 MS.gene69810:CDS
AGGAAGATACTTTGAATGAG+AGG 0.541636 2.4:-16458759 MS.gene69810:CDS
CAGAGATAATAAGACAAAGA+AGG 0.552229 2.4:-16459095 MS.gene69810:CDS
ATATGGTTCAACAGAACTGT+TGG 0.553725 2.4:-16458932 MS.gene69810:CDS
GATGGAAATGGATTTGGCTA+TGG 0.555092 2.4:-16458797 MS.gene69810:CDS
GTGTTTGTAGGGTCTGGAGT+TGG 0.561500 2.4:+16459165 None:intergenic
TCAGCTACCACATACCCTAC+TGG 0.567101 2.4:+16458991 None:intergenic
TGAGAAGCCAGTAGGGTATG+TGG 0.573091 2.4:-16458998 MS.gene69810:CDS
TTGGTGGCTCGGCATTTGCA+AGG 0.573166 2.4:+16459061 None:intergenic
AAATTCTGGCTCAGAAGATG+TGG 0.574456 2.4:+16459188 None:intergenic
ATCAAGCTAAGGCTAGGTGC+AGG 0.575411 2.4:-16458732 MS.gene69810:CDS
ATTAACCTTGAGAAGCCAGT+AGG 0.578990 2.4:-16459006 MS.gene69810:CDS
TGAGAGGCTTGATCAAGCTA+AGG 0.591500 2.4:-16458743 MS.gene69810:CDS
TGTGAGGAATGTTGAAGCCA+TGG 0.619146 2.4:+16459329 None:intergenic
GGCTTGATCAAGCTAAGGCT+AGG 0.619994 2.4:-16458738 MS.gene69810:CDS
TTAACCTTGAGAAGCCAGTA+GGG 0.629422 2.4:-16459005 MS.gene69810:CDS
CATACCCTACTGGCTTCTCA+AGG 0.644603 2.4:+16459001 None:intergenic
AAGAGATTGTGTAGATGATG+AGG 0.673918 2.4:+16459297 None:intergenic
GATGAGGTGCAGGATATGTG+AGG 0.712566 2.4:+16459313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGAAGAAGTGTTTTT+TGG + chr2.4:16458802-16458821 None:intergenic 20.0%
! TTCCAACCTATTTCAAAATT+TGG + chr2.4:16458990-16459009 None:intergenic 25.0%
!!! ATTTTGAAATAGGTTGGAAA+AGG - chr2.4:16458991-16459010 MS.gene69810:CDS 25.0%
CAACCTATTTCAAAATTTGG+TGG + chr2.4:16458987-16459006 None:intergenic 30.0%
CAGAGATAATAAGACAAAGA+AGG - chr2.4:16458934-16458953 MS.gene69810:CDS 30.0%
GAACTTATGAAGAAGGAATA+TGG - chr2.4:16459080-16459099 MS.gene69810:CDS 30.0%
TCAGAAGATGTGGAATTTAA+TGG + chr2.4:16458834-16458853 None:intergenic 30.0%
TGTTCATTGTAGTGTTTGTA+GGG + chr2.4:16458878-16458897 None:intergenic 30.0%
TTCTCTATTGGAAATGTTTC+TGG + chr2.4:16458903-16458922 None:intergenic 30.0%
! GATCTTCTTCTTTTCTCTAT+TGG + chr2.4:16458915-16458934 None:intergenic 30.0%
! TATTTCAAAATTTGGTGGCT+CGG + chr2.4:16458982-16459001 None:intergenic 30.0%
! TGAAAGTTGTGAAGATGTTT+TGG + chr2.4:16458763-16458782 None:intergenic 30.0%
!!! CACCAAATTTTGAAATAGGT+TGG - chr2.4:16458985-16459004 MS.gene69810:CDS 30.0%
!!! TTGAACTCTTTAGAGTTTTG+AGG - chr2.4:16459207-16459226 MS.gene69810:CDS 30.0%
AAGAGATTGTGTAGATGATG+AGG + chr2.4:16458735-16458754 None:intergenic 35.0%
AGGAAGATACTTTGAATGAG+AGG - chr2.4:16459270-16459289 MS.gene69810:CDS 35.0%
ATATGGTTCAACAGAACTGT+TGG - chr2.4:16459097-16459116 MS.gene69810:CDS 35.0%
CTGTTCATTGTAGTGTTTGT+AGG + chr2.4:16458879-16458898 None:intergenic 35.0%
TATGGTGAAAGCTTCTGTTA+AGG - chr2.4:16459250-16459269 MS.gene69810:CDS 35.0%
!! GCAGAAATTTTGAGAGATGA+AGG - chr2.4:16459161-16459180 MS.gene69810:CDS 35.0%
!!! AGCTGATTTTTTGGAGAAGT+TGG - chr2.4:16459055-16459074 MS.gene69810:CDS 35.0%
!!! AGTTTTGAGGTTGATGGAAA+TGG - chr2.4:16459220-16459239 MS.gene69810:CDS 35.0%
!!! CTTTAGAGTTTTGAGGTTGA+TGG - chr2.4:16459214-16459233 MS.gene69810:CDS 35.0%
!!! GAGCCACCAAATTTTGAAAT+AGG - chr2.4:16458981-16459000 MS.gene69810:CDS 35.0%
!!! GTATGTGGTAGCTGATTTTT+TGG - chr2.4:16459046-16459065 MS.gene69810:CDS 35.0%
!!! TGATTTTTTGGAGAAGTTGG+AGG - chr2.4:16459058-16459077 MS.gene69810:CDS 35.0%
ACAGAACTGTTGGCAAAAGT+TGG - chr2.4:16459107-16459126 MS.gene69810:CDS 40.0%
AGGAAAAGTTGAGGAGAGAA+TGG - chr2.4:16459181-16459200 MS.gene69810:CDS 40.0%
ATTAACCTTGAGAAGCCAGT+AGG - chr2.4:16459023-16459042 MS.gene69810:CDS 40.0%
ATTGTAGTGTTTGTAGGGTC+TGG + chr2.4:16458873-16458892 None:intergenic 40.0%
GAGAGATGAAGGAAAAGTTG+AGG - chr2.4:16459172-16459191 MS.gene69810:CDS 40.0%
GTTGGAGGAACTTATGAAGA+AGG - chr2.4:16459073-16459092 MS.gene69810:CDS 40.0%
TTAACCTTGAGAAGCCAGTA+GGG - chr2.4:16459024-16459043 MS.gene69810:CDS 40.0%
! GATGGAAATGGATTTGGCTA+TGG - chr2.4:16459232-16459251 MS.gene69810:CDS 40.0%
!! AAATTCTGGCTCAGAAGATG+TGG + chr2.4:16458844-16458863 None:intergenic 40.0%
!! GAGGTTGATGGAAATGGATT+TGG - chr2.4:16459226-16459245 MS.gene69810:CDS 40.0%
TGAGAGGCTTGATCAAGCTA+AGG - chr2.4:16459286-16459305 MS.gene69810:CDS 45.0%
TTGTGTAGATGATGAGGTGC+AGG + chr2.4:16458729-16458748 None:intergenic 45.0%
!! GCATTTGCAAGGTCGGTTTT+CGG + chr2.4:16458960-16458979 None:intergenic 45.0%
ATCAAGCTAAGGCTAGGTGC+AGG - chr2.4:16459297-16459316 MS.gene69810:CDS 50.0%
GATGAGGTGCAGGATATGTG+AGG + chr2.4:16458719-16458738 None:intergenic 50.0%
GGCTTGATCAAGCTAAGGCT+AGG - chr2.4:16459291-16459310 MS.gene69810:CDS 50.0%
TCAGCTACCACATACCCTAC+TGG + chr2.4:16459041-16459060 None:intergenic 50.0%
TGAGAAGCCAGTAGGGTATG+TGG - chr2.4:16459031-16459050 MS.gene69810:CDS 50.0%
! GGGTCTGGAGTTGGAAATTC+TGG + chr2.4:16458858-16458877 None:intergenic 50.0%
! GTGTTTGTAGGGTCTGGAGT+TGG + chr2.4:16458867-16458886 None:intergenic 50.0%
!! CATACCCTACTGGCTTCTCA+AGG + chr2.4:16459031-16459050 None:intergenic 50.0%
! TGGCTCGGCATTTGCAAGGT+CGG + chr2.4:16458967-16458986 None:intergenic 55.0%
! TTGGTGGCTCGGCATTTGCA+AGG + chr2.4:16458971-16458990 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 16458702 16459349 16458702 ID=MS.gene69810
chr2.4 mRNA 16458702 16459349 16458702 ID=MS.gene69810.t1;Parent=MS.gene69810
chr2.4 exon 16458702 16459349 16458702 ID=MS.gene69810.t1.exon1;Parent=MS.gene69810.t1
chr2.4 CDS 16458702 16459349 16458702 ID=cds.MS.gene69810.t1;Parent=MS.gene69810.t1
Gene Sequence

>MS.gene69810

ATGGCTTCAACATTCCTCACATATCCTGCACCTCATCATCTACACAATCTCTTTCAAACCAAAACATCTTCACAACTTTCAACTCACAGCAACAAAACCAAAAAACACTTCTTCATAAAATGCACTACTCCATTAAATTCCACATCTTCTGAGCCAGAATTTCCAACTCCAGACCCTACAAACACTACAATGAACAGTCCAGAAACATTTCCAATAGAGAAAAGAAGAAGATCAGAGATAATAAGACAAAGAAGGCCGAAAACCGACCTTGCAAATGCCGAGCCACCAAATTTTGAAATAGGTTGGAAAAGGACAAAAGAGATTAACCTTGAGAAGCCAGTAGGGTATGTGGTAGCTGATTTTTTGGAGAAGTTGGAGGAACTTATGAAGAAGGAATATGGTTCAACAGAACTGTTGGCAAAAGTTGGTGAAATTGTTGCTGAAAGAGCAAGAGAAGAAGCAGAAATTTTGAGAGATGAAGGAAAAGTTGAGGAGAGAATGGTTGTTGAACTCTTTAGAGTTTTGAGGTTGATGGAAATGGATTTGGCTATGGTGAAAGCTTCTGTTAAGGAAGATACTTTGAATGAGAGGCTTGATCAAGCTAAGGCTAGGTGCAGGCAAGCTATACTTGTTTGTTATTCCTTTTGA

Protein sequence

>MS.gene69810.t1

MASTFLTYPAPHHLHNLFQTKTSSQLSTHSNKTKKHFFIKCTTPLNSTSSEPEFPTPDPTNTTMNSPETFPIEKRRRSEIIRQRRPKTDLANAEPPNFEIGWKRTKEINLEKPVGYVVADFLEKLEELMKKEYGSTELLAKVGEIVAERAREEAEILRDEGKVEERMVVELFRVLRLMEMDLAMVKASVKEDTLNERLDQAKARCRQAILVCYSF