Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90404.t1 | XP_003595873.2 | 98.1 | 700 | 13 | 0 | 1 | 700 | 45 | 744 | 0.00E+00 | 1332.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90404.t1 | Q6NLC1 | 68.7 | 648 | 187 | 6 | 53 | 698 | 60 | 693 | 1.5e-244 | 847.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90404.t1 | G7IT31 | 98.1 | 700 | 13 | 0 | 1 | 700 | 45 | 744 | 0.0e+00 | 1332.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene90404 | 0.832669 | 8.29E-56 | -1.69E-46 |
| MS.gene049173 | MS.gene90404 | 0.809621 | 1.73E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene90404 | 0.824248 | 8.95E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene90404 | 0.842619 | 2.32E-58 | -1.69E-46 |
| MS.gene049285 | MS.gene90404 | 0.847539 | 1.08E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene90404 | 0.820471 | 6.74E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene90404 | 0.821812 | 3.31E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene90404 | 0.82151 | 3.89E-53 | -1.69E-46 |
| MS.gene05010 | MS.gene90404 | 0.829519 | 4.92E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene90404 | 0.805336 | 1.40E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene90404 | 0.816245 | 6.11E-52 | -1.69E-46 |
| MS.gene050444 | MS.gene90404 | 0.823404 | 1.41E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene90404 | 0.811038 | 8.55E-51 | -1.69E-46 |
| MS.gene050518 | MS.gene90404 | 0.806118 | 9.61E-50 | -1.69E-46 |
| MS.gene050520 | MS.gene90404 | 0.812197 | 4.79E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene90404 | 0.818153 | 2.27E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene90404 | 0.808407 | 3.15E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene90404 | 0.812455 | 4.20E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene90404 | 0.846984 | 1.54E-59 | -1.69E-46 |
| MS.gene05080 | MS.gene90404 | 0.820817 | 5.61E-53 | -1.69E-46 |
| MS.gene05081 | MS.gene90404 | 0.815817 | 7.61E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene90404 | 0.836201 | 1.07E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene90404 | 0.834742 | 2.51E-56 | -1.69E-46 |
| MS.gene050904 | MS.gene90404 | 0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene051228 | MS.gene90404 | 0.81773 | 2.83E-52 | -1.69E-46 |
| MS.gene051268 | MS.gene90404 | 0.810918 | 9.08E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene90404 | 0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene051551 | MS.gene90404 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene90404 | 0.826521 | 2.59E-54 | -1.69E-46 |
| MS.gene051734 | MS.gene90404 | 0.801194 | 1.01E-48 | -1.69E-46 |
| MS.gene051838 | MS.gene90404 | 0.816935 | 4.28E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene90404 | 0.84043 | 8.74E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene90404 | 0.828402 | 9.17E-55 | -1.69E-46 |
| MS.gene051949 | MS.gene90404 | 0.828118 | 1.07E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene90404 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene052484 | MS.gene90404 | 0.812926 | 3.32E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene90404 | 0.809109 | 2.23E-50 | -1.69E-46 |
| MS.gene052506 | MS.gene90404 | 0.807107 | 5.94E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene90404 | 0.816219 | 6.19E-52 | -1.69E-46 |
| MS.gene052870 | MS.gene90404 | 0.850444 | 1.69E-60 | -1.69E-46 |
| MS.gene052872 | MS.gene90404 | 0.849522 | 3.06E-60 | -1.69E-46 |
| MS.gene052966 | MS.gene90404 | 0.84424 | 8.55E-59 | -1.69E-46 |
| MS.gene053103 | MS.gene90404 | 0.810824 | 9.51E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene90404 | 0.826259 | 2.99E-54 | -1.69E-46 |
| MS.gene053207 | MS.gene90404 | 0.821363 | 4.20E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene90404 | 0.807492 | 4.92E-50 | -1.69E-46 |
| MS.gene053286 | MS.gene90404 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene053449 | MS.gene90404 | 0.81525 | 1.02E-51 | -1.69E-46 |
| MS.gene053479 | MS.gene90404 | 0.820149 | 7.99E-53 | -1.69E-46 |
| MS.gene053574 | MS.gene90404 | 0.811315 | 7.45E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene90404 | 0.834996 | 2.17E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene90404 | 0.867306 | 1.51E-65 | -1.69E-46 |
| MS.gene053916 | MS.gene90404 | 0.869322 | 3.38E-66 | -1.69E-46 |
| MS.gene054595 | MS.gene90404 | 0.821713 | 3.49E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene90404 | 0.860259 | 2.34E-63 | -1.69E-46 |
| MS.gene054642 | MS.gene90404 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene05494 | MS.gene90404 | 0.864744 | 9.75E-65 | -1.69E-46 |
| MS.gene054954 | MS.gene90404 | 0.81005 | 1.40E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene90404 | 0.829714 | 4.41E-55 | -1.69E-46 |
| MS.gene055206 | MS.gene90404 | 0.849979 | 2.28E-60 | -1.69E-46 |
| MS.gene055224 | MS.gene90404 | 0.832574 | 8.75E-56 | -1.69E-46 |
| MS.gene055362 | MS.gene90404 | 0.8307 | 2.53E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene90404 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene90404 | 0.8459 | 3.04E-59 | -1.69E-46 |
| MS.gene055722 | MS.gene90404 | 0.839899 | 1.20E-57 | -1.69E-46 |
| MS.gene055748 | MS.gene90404 | 0.838237 | 3.24E-57 | -1.69E-46 |
| MS.gene055750 | MS.gene90404 | 0.838601 | 2.61E-57 | -1.69E-46 |
| MS.gene055751 | MS.gene90404 | 0.839328 | 1.69E-57 | -1.69E-46 |
| MS.gene05589 | MS.gene90404 | 0.800978 | 1.12E-48 | -1.69E-46 |
| MS.gene05592 | MS.gene90404 | 0.835752 | 1.40E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene90404 | 0.863011 | 3.38E-64 | -1.69E-46 |
| MS.gene056374 | MS.gene90404 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene056409 | MS.gene90404 | 0.816951 | 4.24E-52 | -1.69E-46 |
| MS.gene056449 | MS.gene90404 | 0.830299 | 3.18E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene90404 | 0.818151 | 2.28E-52 | -1.69E-46 |
| MS.gene056510 | MS.gene90404 | 0.812325 | 4.49E-51 | -1.69E-46 |
| MS.gene05664 | MS.gene90404 | 0.826136 | 3.20E-54 | -1.69E-46 |
| MS.gene056657 | MS.gene90404 | 0.819108 | 1.38E-52 | -1.69E-46 |
| MS.gene056878 | MS.gene90404 | 0.800313 | 1.53E-48 | -1.69E-46 |
| MS.gene057331 | MS.gene90404 | 0.826405 | 2.76E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene90404 | 0.822853 | 1.90E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene90404 | 0.804733 | 1.87E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene90404 | 0.834994 | 2.17E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene90404 | 0.807631 | 4.60E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene90404 | 0.85387 | 1.80E-61 | -1.69E-46 |
| MS.gene058006 | MS.gene90404 | 0.825494 | 4.55E-54 | -1.69E-46 |
| MS.gene058120 | MS.gene90404 | 0.80232 | 5.93E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene90404 | 0.825263 | 5.16E-54 | -1.69E-46 |
| MS.gene058338 | MS.gene90404 | 0.830064 | 3.62E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene90404 | 0.816347 | 5.80E-52 | -1.69E-46 |
| MS.gene058723 | MS.gene90404 | 0.823411 | 1.41E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene90404 | 0.858521 | 7.78E-63 | -1.69E-46 |
| MS.gene058838 | MS.gene90404 | 0.83348 | 5.21E-56 | -1.69E-46 |
| MS.gene058948 | MS.gene90404 | 0.809979 | 1.45E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene90404 | 0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene90404 | 0.839813 | 1.27E-57 | -1.69E-46 |
| MS.gene05913 | MS.gene90404 | 0.817631 | 2.98E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene90404 | 0.814274 | 1.67E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene90404 | 0.823179 | 1.59E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene90404 | 0.808675 | 2.76E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene90404 | 0.824334 | 8.54E-54 | -1.69E-46 |
| MS.gene059801 | MS.gene90404 | 0.836752 | 7.78E-57 | -1.69E-46 |
| MS.gene059902 | MS.gene90404 | 0.83371 | 4.56E-56 | -1.69E-46 |
| MS.gene060193 | MS.gene90404 | 0.823043 | 1.71E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene90404 | 0.833203 | 6.10E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene90404 | 0.806879 | 6.64E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene90404 | 0.810612 | 1.06E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene90404 | 0.802339 | 5.88E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene90404 | 0.837665 | 4.55E-57 | -1.69E-46 |
| MS.gene06092 | MS.gene90404 | 0.817242 | 3.65E-52 | -1.69E-46 |
| MS.gene06097 | MS.gene90404 | 0.821983 | 3.02E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene90404 | 0.804278 | 2.33E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene90404 | 0.805542 | 1.27E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene90404 | 0.82056 | 6.43E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene90404 | 0.803992 | 2.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90404 | MS.gene33433 | PPI |
| MS.gene90404 | MS.gene33686 | PPI |
| MS.gene90404 | MS.gene068301 | PPI |
| MS.gene90404 | MS.gene24979 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90404.t1 | MTR_2g062790 | 98.143 | 700 | 13 | 0 | 1 | 700 | 45 | 744 | 0.0 | 1398 |
| MS.gene90404.t1 | MTR_1g057830 | 88.889 | 360 | 33 | 1 | 341 | 700 | 12 | 364 | 0.0 | 594 |
| MS.gene90404.t1 | MTR_3g087350 | 29.983 | 577 | 314 | 17 | 140 | 700 | 166 | 668 | 6.48e-58 | 213 |
| MS.gene90404.t1 | MTR_3g087350 | 27.556 | 577 | 306 | 18 | 145 | 681 | 825 | 1329 | 1.66e-45 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90404.t1 | AT1G54350 | 65.665 | 699 | 204 | 8 | 4 | 698 | 27 | 693 | 0.0 | 886 |
| MS.gene90404.t1 | AT4G39850 | 29.443 | 574 | 316 | 16 | 140 | 698 | 168 | 667 | 8.22e-57 | 210 |
| MS.gene90404.t1 | AT4G39850 | 28.549 | 634 | 357 | 18 | 80 | 683 | 762 | 1329 | 5.46e-49 | 186 |
| MS.gene90404.t1 | AT4G39850 | 29.443 | 574 | 316 | 16 | 140 | 698 | 168 | 667 | 8.85e-57 | 210 |
| MS.gene90404.t1 | AT4G39850 | 27.932 | 648 | 357 | 19 | 80 | 683 | 762 | 1343 | 1.92e-46 | 179 |
| MS.gene90404.t1 | AT4G39850 | 29.443 | 574 | 316 | 16 | 140 | 698 | 168 | 667 | 9.01e-57 | 210 |
| MS.gene90404.t1 | AT4G39850 | 28.436 | 633 | 358 | 17 | 80 | 683 | 762 | 1328 | 1.35e-49 | 188 |
| MS.gene90404.t1 | AT4G39850 | 29.443 | 574 | 316 | 16 | 140 | 698 | 168 | 667 | 9.01e-57 | 210 |
| MS.gene90404.t1 | AT4G39850 | 28.436 | 633 | 358 | 17 | 80 | 683 | 762 | 1328 | 1.35e-49 | 188 |
Find 134 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAATGGAAGGCCTTTC+AGG | 0.154189 | 2.3:+31663669 | MS.gene90404:CDS |
| GAACAAAAGAAAACCTTATC+TGG | 0.214012 | 2.3:-31663749 | None:intergenic |
| TCAAAAGCCGTACATGGTTT+TGG | 0.237570 | 2.3:+31671483 | MS.gene90404:CDS |
| AAATTTCTTGCAAGAGAAAA+AGG | 0.250601 | 2.3:+31667298 | MS.gene90404:CDS |
| GGAAAGTGGCAGCACCTTAT+TGG | 0.261757 | 2.3:+31664357 | MS.gene90404:CDS |
| TAAATCTCTCTCGGATTCTT+TGG | 0.269966 | 2.3:+31670986 | MS.gene90404:CDS |
| GTTCTTCTCATATACTCTAT+TGG | 0.270925 | 2.3:+31667119 | MS.gene90404:CDS |
| AACAGCCCTTTCTTTCTCTT+TGG | 0.272720 | 2.3:+31667022 | MS.gene90404:CDS |
| AGGCGAGTTTGATGATATTT+TGG | 0.273248 | 2.3:+31670950 | MS.gene90404:intron |
| TACTACTTGGGTAGCTTTGC+TGG | 0.286162 | 2.3:+31666088 | MS.gene90404:CDS |
| TCATCTGCATTGGAATCTAA+TGG | 0.289555 | 2.3:+31671044 | MS.gene90404:CDS |
| GCTGATTTCTTCTAGAAATC+TGG | 0.291668 | 2.3:+31669445 | MS.gene90404:CDS |
| CAAACAATGAACAGCCAAAT+TGG | 0.291996 | 2.3:+31674281 | MS.gene90404:CDS |
| TTATGGACTTGTACGGGTTC+GGG | 0.324633 | 2.3:+31667334 | MS.gene90404:CDS |
| GTTATGGACTTGTACGGGTT+CGG | 0.326593 | 2.3:+31667333 | MS.gene90404:CDS |
| AACATCTTTATTAAGAGTTA+TGG | 0.330581 | 2.3:+31671303 | MS.gene90404:CDS |
| GCTGAGTCAATTGCTTTCTA+TGG | 0.332810 | 2.3:+31667362 | MS.gene90404:CDS |
| TAAGAGTTATGGCTGGTCTA+TGG | 0.333129 | 2.3:+31671314 | MS.gene90404:CDS |
| GTGGGTTCGATGTGCCAATT+TGG | 0.336932 | 2.3:-31674295 | None:intergenic |
| AAGATCATATACTATGTAAA+AGG | 0.341820 | 2.3:+31671352 | MS.gene90404:CDS |
| ATGGTCTAGTGTTCTCTCTC+TGG | 0.347611 | 2.3:+31673725 | MS.gene90404:CDS |
| TTTCATTGAGATGGAGGTCT+TGG | 0.352402 | 2.3:+31666880 | MS.gene90404:CDS |
| GCTACATATTACCTCGGTTC+GGG | 0.358370 | 2.3:+31673685 | MS.gene90404:CDS |
| AACAAATGTGTCTCTGAATC+AGG | 0.363221 | 2.3:+31663704 | MS.gene90404:CDS |
| AACAATTACTTTATCCAACT+TGG | 0.371885 | 2.3:+31671518 | MS.gene90404:CDS |
| CAAGCAAGACTGCAGCTTGC+TGG | 0.374990 | 2.3:+31664398 | MS.gene90404:CDS |
| AGTATTCACATCTGAACTAA+TGG | 0.378490 | 2.3:-31671395 | None:intergenic |
| GCTCTCACTTTGGCTACTAC+TGG | 0.383712 | 2.3:+31664428 | MS.gene90404:CDS |
| GGCTACATATTACCTCGGTT+CGG | 0.388740 | 2.3:+31673684 | MS.gene90404:CDS |
| AAGGAAGCAGACTTTCGTTA+TGG | 0.392421 | 2.3:+31667317 | MS.gene90404:CDS |
| TCATCAACAATTCGCTGGTC+TGG | 0.393279 | 2.3:-31666981 | None:intergenic |
| CGAGCTGATAAAGATCTTAG+AGG | 0.394660 | 2.3:+31673650 | MS.gene90404:CDS |
| AGAGAAAGAAAGGGCTGTTC+CGG | 0.394846 | 2.3:-31667018 | None:intergenic |
| TCTGCTGTCATTAATCGGTT+AGG | 0.396603 | 2.3:+31669647 | MS.gene90404:CDS |
| GTTGCTCCCATGTATTTCTC+TGG | 0.404905 | 2.3:+31669521 | MS.gene90404:CDS |
| TTATTGGAGTTCTGATGATA+AGG | 0.408989 | 2.3:+31664373 | MS.gene90404:CDS |
| TAACCAATTTAACAGATAAC+AGG | 0.410263 | 2.3:+31671262 | MS.gene90404:intron |
| CATCTGCATTGGAATCTAAT+GGG | 0.415567 | 2.3:+31671045 | MS.gene90404:CDS |
| ACTGAATGAGATCAAGTCTA+TGG | 0.420267 | 2.3:-31667060 | None:intergenic |
| CTTTACAAGTTCATCTGCAT+TGG | 0.434448 | 2.3:+31671034 | MS.gene90404:CDS |
| AAGCACTTATAGCTTGAAAC+TGG | 0.436695 | 2.3:-31669622 | None:intergenic |
| AAAGCAGCTACTTTACTACT+TGG | 0.443578 | 2.3:+31666075 | MS.gene90404:CDS |
| GAGAAACTCTTTCATTGAGA+TGG | 0.445852 | 2.3:+31666871 | MS.gene90404:CDS |
| ACAATTACTTTATCCAACTT+GGG | 0.452027 | 2.3:+31671519 | MS.gene90404:CDS |
| TGTTTATCACTATCTGATGT+TGG | 0.452089 | 2.3:-31671556 | None:intergenic |
| TTATCATCAGAACTCCAATA+AGG | 0.455180 | 2.3:-31664371 | None:intergenic |
| TAGGATTCTGTCGTGGAAGT+TGG | 0.460665 | 2.3:-31674239 | None:intergenic |
| AAAACCTCAACTAGCTCTAC+TGG | 0.460778 | 2.3:+31673788 | MS.gene90404:CDS |
| AAATCCATCAGCAGAAAATC+CGG | 0.460996 | 2.3:+31671445 | MS.gene90404:CDS |
| TTCAAATTCTTCCTCCTGAA+AGG | 0.461386 | 2.3:-31663683 | None:intergenic |
| AGACTTATCATTGACAATCA+AGG | 0.461558 | 2.3:+31671151 | MS.gene90404:CDS |
| TGACGGTGTGGTGGAAGTGC+AGG | 0.463646 | 2.3:+31664295 | MS.gene90404:CDS |
| AGAGTGCCAAAGAGAAAGAA+AGG | 0.464970 | 2.3:-31667028 | None:intergenic |
| CCTACCTCAAAAGCCGTACA+TGG | 0.467676 | 2.3:+31671477 | MS.gene90404:CDS |
| ACTTTCGTTATGGACTTGTA+CGG | 0.468756 | 2.3:+31667327 | MS.gene90404:CDS |
| ATGGCTGGTCTATGGAGAAC+TGG | 0.471645 | 2.3:+31671322 | MS.gene90404:CDS |
| GCTACTACTGGAATTAGTGT+TGG | 0.474431 | 2.3:+31664440 | MS.gene90404:CDS |
| GGAGTTCAAGAATACCTCAT+TGG | 0.477708 | 2.3:-31673838 | None:intergenic |
| TGGTCTAGTGTTCTCTCTCT+GGG | 0.488358 | 2.3:+31673726 | MS.gene90404:CDS |
| TGGCTGTTCATTGTTTGAAT+CGG | 0.491436 | 2.3:-31674275 | None:intergenic |
| TCATATACTATGTAAAAGGC+GGG | 0.491506 | 2.3:+31671356 | MS.gene90404:CDS |
| TTACATGGATCGCTACTTGA+AGG | 0.492972 | 2.3:+31666914 | MS.gene90404:CDS |
| TACGAAAAGATTGCAGCAGC+GGG | 0.497494 | 2.3:+31674178 | MS.gene90404:CDS |
| TCTGTCGTGGAAGTTGGATA+AGG | 0.498346 | 2.3:-31674233 | None:intergenic |
| CCCATTATGCCCCTTTCCAA+TGG | 0.499905 | 2.3:+31663656 | None:intergenic |
| GAAAGGCCTTCCATTGGAAA+GGG | 0.502321 | 2.3:-31663666 | None:intergenic |
| CCTCCTGAAAGGCCTTCCAT+TGG | 0.503907 | 2.3:-31663672 | None:intergenic |
| ATCTTAGAGGATGTCCGTCT+TGG | 0.514615 | 2.3:+31673663 | MS.gene90404:CDS |
| AATGTTTCATGTTTCTCTTG+TGG | 0.516449 | 2.3:-31671074 | None:intergenic |
| CTGTTCATTGTTTGAATCGG+TGG | 0.517678 | 2.3:-31674272 | None:intergenic |
| CATATTACCTCGGTTCGGGT+TGG | 0.517683 | 2.3:+31673689 | MS.gene90404:CDS |
| CTTTCGTTATGGACTTGTAC+GGG | 0.519129 | 2.3:+31667328 | MS.gene90404:CDS |
| GGTTGGATTCAACACATGAA+TGG | 0.519369 | 2.3:+31673706 | MS.gene90404:CDS |
| CTTCTCATATACTCTATTGG+TGG | 0.523116 | 2.3:+31667122 | MS.gene90404:CDS |
| GGCATAACATACATTAGTGT+TGG | 0.526211 | 2.3:+31674199 | MS.gene90404:CDS |
| TAAAGAGGATTCGCGTCTAG+TGG | 0.528037 | 2.3:-31674314 | None:intergenic |
| GATGATCTAAGTTCTTTCAC+CGG | 0.529116 | 2.3:+31666999 | MS.gene90404:CDS |
| AACAAAAGAAAACCTTATCT+GGG | 0.530791 | 2.3:-31663748 | None:intergenic |
| TGAAAGGCCTTCCATTGGAA+AGG | 0.534046 | 2.3:-31663667 | None:intergenic |
| GAGTCAATTGCTTTCTATGG+TGG | 0.534909 | 2.3:+31667365 | MS.gene90404:CDS |
| TTATCTGGGTCAGTTGGCAG+TGG | 0.540962 | 2.3:-31663734 | None:intergenic |
| TTATGCCCCTTTCCAATGGA+AGG | 0.541333 | 2.3:+31663660 | None:intergenic |
| TAGCTTTGCTGGAGGAATCC+CGG | 0.546278 | 2.3:+31666099 | MS.gene90404:CDS |
| ATAACAGGACCAAGTGGAAG+TGG | 0.548195 | 2.3:+31671277 | MS.gene90404:CDS |
| TGTGCAGAAAGATGAGAAAG+TGG | 0.549877 | 2.3:+31664262 | MS.gene90404:intron |
| ATGGGAGCAACAACAGCAGC+AGG | 0.550352 | 2.3:-31669509 | None:intergenic |
| AAGCAACTCTAAATCTCTCT+CGG | 0.551088 | 2.3:+31670977 | MS.gene90404:CDS |
| AAAACCTTATCTGGGTCAGT+TGG | 0.552992 | 2.3:-31663740 | None:intergenic |
| GAGTGCCAAAGAGAAAGAAA+GGG | 0.554199 | 2.3:-31667027 | None:intergenic |
| TCTTTATTAAGAGTTATGGC+TGG | 0.559998 | 2.3:+31671307 | MS.gene90404:CDS |
| TTAGATCATCAACAATTCGC+TGG | 0.561218 | 2.3:-31666986 | None:intergenic |
| ACTGCCAACTGACCCAGATA+AGG | 0.562901 | 2.3:+31663736 | MS.gene90404:CDS |
| CAATTACTTTATCCAACTTG+GGG | 0.565844 | 2.3:+31671520 | MS.gene90404:CDS |
| AAACTCTTTCATTGAGATGG+AGG | 0.566123 | 2.3:+31666874 | MS.gene90404:CDS |
| TTGGATGACGAGGTATTACA+TGG | 0.566640 | 2.3:+31666899 | MS.gene90404:CDS |
| AAGCAGCTACTTTACTACTT+GGG | 0.566859 | 2.3:+31666076 | MS.gene90404:CDS |
| ATCATATACTATGTAAAAGG+CGG | 0.569322 | 2.3:+31671355 | MS.gene90404:CDS |
| GGTCTATGGAGAACTGGAAA+CGG | 0.570774 | 2.3:+31671328 | MS.gene90404:CDS |
| AATGACATAGCGGTAACCAT+TGG | 0.575192 | 2.3:-31669477 | None:intergenic |
| AAAGAGGATTCGCGTCTAGT+GGG | 0.575584 | 2.3:-31674313 | None:intergenic |
| GTGATATATCTGAAGATCGT+GGG | 0.577030 | 2.3:+31671422 | MS.gene90404:CDS |
| ATGAGAAGAACAACAAAGAG+TGG | 0.577778 | 2.3:-31667107 | None:intergenic |
| AGAAGTAAGTGACGGTGTGG+TGG | 0.578544 | 2.3:+31664286 | MS.gene90404:CDS |
| AGAGTACCCAAAACCATGTA+CGG | 0.579041 | 2.3:-31671490 | None:intergenic |
| ACGACATCATCACCCCAAGT+TGG | 0.582232 | 2.3:-31671532 | None:intergenic |
| AATCTGGAGTTCTTCACCAA+TGG | 0.584185 | 2.3:+31669461 | MS.gene90404:CDS |
| ATCGACAAGTGCACTTGATG+AGG | 0.590195 | 2.3:+31673815 | MS.gene90404:CDS |
| AGATGCAACTTCTTCTGCAG+CGG | 0.593320 | 2.3:+31667399 | MS.gene90404:CDS |
| GTCTTGGCTACATATTACCT+CGG | 0.603287 | 2.3:+31673679 | MS.gene90404:CDS |
| TGCACTTGATGAGGCCAATG+AGG | 0.607522 | 2.3:+31673824 | MS.gene90404:CDS |
| CGTGATATATCTGAAGATCG+TGG | 0.617035 | 2.3:+31671421 | MS.gene90404:CDS |
| AATCGGTGGAAGATATGCGT+AGG | 0.617074 | 2.3:-31674258 | None:intergenic |
| GTACGAAAAGATTGCAGCAG+CGG | 0.617171 | 2.3:+31674177 | MS.gene90404:CDS |
| CCAATGGAAGGCCTTTCAGG+AGG | 0.619194 | 2.3:+31663672 | MS.gene90404:CDS |
| TCGTCCAGTAGAGCTAGTTG+AGG | 0.625390 | 2.3:-31673792 | None:intergenic |
| AAAGTGGGAGAAGTAAGTGA+CGG | 0.626937 | 2.3:+31664278 | MS.gene90404:CDS |
| CATATACTATGTAAAAGGCG+GGG | 0.635401 | 2.3:+31671357 | MS.gene90404:CDS |
| GTGCAGAAAGATGAGAAAGT+GGG | 0.636119 | 2.3:+31664263 | MS.gene90404:intron |
| AGAAGAACAACAAAGAGTGG+AGG | 0.645391 | 2.3:-31667104 | None:intergenic |
| GTAATAAGTGTAGAATCACT+TGG | 0.648547 | 2.3:-31671128 | None:intergenic |
| CATACATTAGTGTTGGCCAT+CGG | 0.648954 | 2.3:+31674206 | MS.gene90404:CDS |
| AAAGGCCTTCCATTGGAAAG+GGG | 0.656936 | 2.3:-31663665 | None:intergenic |
| TACTTGGGTAGCTTTGCTGG+AGG | 0.656943 | 2.3:+31666091 | MS.gene90404:CDS |
| GACGGTGTGGTGGAAGTGCA+GGG | 0.662864 | 2.3:+31664296 | MS.gene90404:CDS |
| GATGGAGGTCTTGGATGACG+AGG | 0.669239 | 2.3:+31666889 | MS.gene90404:CDS |
| TAACAGATAACAGGACCAAG+TGG | 0.669592 | 2.3:+31671271 | MS.gene90404:intron |
| GAGGTAATATGTAGCCAAGA+CGG | 0.673624 | 2.3:-31673677 | None:intergenic |
| CGATCTTCAGATATATCACG+AGG | 0.678508 | 2.3:-31671418 | None:intergenic |
| GTTGAATCCAACCCGAACCG+AGG | 0.684589 | 2.3:-31673696 | None:intergenic |
| AGTTGGATAAGGTTGACCGA+TGG | 0.689538 | 2.3:-31674222 | None:intergenic |
| GGGAGAAGTAAGTGACGGTG+TGG | 0.698820 | 2.3:+31664283 | MS.gene90404:CDS |
| ATATGCGTAGGATTCTGTCG+TGG | 0.730796 | 2.3:-31674246 | None:intergenic |
| GAAGAACTTGAATGACATAG+CGG | 0.742392 | 2.3:-31669487 | None:intergenic |
| CAATGAGAGAAAAGTCACCG+AGG | 0.781919 | 2.3:-31669595 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 31663662 | 31674345 | 31663662 | ID=MS.gene90404 |
| chr2.3 | mRNA | 31663662 | 31674345 | 31663662 | ID=MS.gene90404.t1;Parent=MS.gene90404 |
| chr2.3 | exon | 31663662 | 31663757 | 31663662 | ID=MS.gene90404.t1.exon1;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31663662 | 31663757 | 31663662 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31664269 | 31664503 | 31664269 | ID=MS.gene90404.t1.exon2;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31664269 | 31664503 | 31664269 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31666053 | 31666120 | 31666053 | ID=MS.gene90404.t1.exon3;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31666053 | 31666120 | 31666053 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31666846 | 31667172 | 31666846 | ID=MS.gene90404.t1.exon4;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31666846 | 31667172 | 31666846 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31667284 | 31667448 | 31667284 | ID=MS.gene90404.t1.exon5;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31667284 | 31667448 | 31667284 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31669440 | 31669668 | 31669440 | ID=MS.gene90404.t1.exon6;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31669440 | 31669668 | 31669440 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31670952 | 31671190 | 31670952 | ID=MS.gene90404.t1.exon7;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31670952 | 31671190 | 31670952 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31671277 | 31671583 | 31671277 | ID=MS.gene90404.t1.exon8;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31671277 | 31671583 | 31671277 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31673586 | 31673845 | 31673586 | ID=MS.gene90404.t1.exon9;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31673586 | 31673845 | 31673586 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| chr2.3 | exon | 31674169 | 31674345 | 31674169 | ID=MS.gene90404.t1.exon10;Parent=MS.gene90404.t1 |
| chr2.3 | CDS | 31674169 | 31674345 | 31674169 | ID=cds.MS.gene90404.t1;Parent=MS.gene90404.t1 |
| Gene Sequence |
| Protein sequence |
