Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96331.t1 | XP_003610020.1 | 55.3 | 264 | 17 | 4 | 1 | 163 | 1 | 264 | 5.90E-55 | 223.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96331.t1 | Q9SV15 | 61.5 | 122 | 33 | 4 | 56 | 163 | 144 | 265 | 6.6e-31 | 135.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96331.t1 | B3U396 | 55.3 | 264 | 17 | 4 | 1 | 163 | 1 | 264 | 4.2e-55 | 223.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene96331.t1 | TF | WRKY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene96331 | -0.836238 | 1.05E-56 | -1.69E-46 |
| MS.gene049151 | MS.gene96331 | -0.835375 | 1.74E-56 | -1.69E-46 |
| MS.gene049153 | MS.gene96331 | -0.830382 | 3.03E-55 | -1.69E-46 |
| MS.gene049260 | MS.gene96331 | -0.861972 | 7.04E-64 | -1.69E-46 |
| MS.gene049297 | MS.gene96331 | -0.858132 | 1.02E-62 | -1.69E-46 |
| MS.gene04933 | MS.gene96331 | -0.816039 | 6.79E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene96331 | -0.811765 | 5.94E-51 | -1.69E-46 |
| MS.gene049351 | MS.gene96331 | -0.803924 | 2.76E-49 | -1.69E-46 |
| MS.gene049599 | MS.gene96331 | 0.854609 | 1.10E-61 | -1.69E-46 |
| MS.gene049758 | MS.gene96331 | -0.900432 | 7.37E-78 | -1.69E-46 |
| MS.gene049910 | MS.gene96331 | -0.812975 | 3.23E-51 | -1.69E-46 |
| MS.gene049915 | MS.gene96331 | -0.816551 | 5.22E-52 | -1.69E-46 |
| MS.gene050099 | MS.gene96331 | -0.826103 | 3.26E-54 | -1.69E-46 |
| MS.gene050128 | MS.gene96331 | -0.831906 | 1.28E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene96331 | -0.821643 | 3.62E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene96331 | -0.834531 | 2.84E-56 | -1.69E-46 |
| MS.gene050228 | MS.gene96331 | -0.82254 | 2.24E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene96331 | -0.863088 | 3.19E-64 | -1.69E-46 |
| MS.gene050319 | MS.gene96331 | 0.820849 | 5.52E-53 | -1.69E-46 |
| MS.gene050330 | MS.gene96331 | 0.874804 | 5.07E-68 | -1.69E-46 |
| MS.gene050397 | MS.gene96331 | -0.815757 | 7.85E-52 | -1.69E-46 |
| MS.gene050529 | MS.gene96331 | -0.82503 | 5.85E-54 | -1.69E-46 |
| MS.gene050558 | MS.gene96331 | -0.834006 | 3.85E-56 | -1.69E-46 |
| MS.gene050623 | MS.gene96331 | 0.872062 | 4.24E-67 | -1.69E-46 |
| MS.gene050642 | MS.gene96331 | -0.859294 | 4.57E-63 | -1.69E-46 |
| MS.gene050762 | MS.gene96331 | -0.803109 | 4.08E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene96331 | -0.806678 | 7.32E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene96331 | -0.809855 | 1.54E-50 | -1.69E-46 |
| MS.gene05119 | MS.gene96331 | 0.844419 | 7.65E-59 | -1.69E-46 |
| MS.gene051240 | MS.gene96331 | -0.828775 | 7.45E-55 | -1.69E-46 |
| MS.gene051257 | MS.gene96331 | -0.802008 | 6.88E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene96331 | -0.83184 | 1.33E-55 | -1.69E-46 |
| MS.gene051662 | MS.gene96331 | -0.869334 | 3.35E-66 | -1.69E-46 |
| MS.gene051663 | MS.gene96331 | -0.85781 | 1.27E-62 | -1.69E-46 |
| MS.gene051789 | MS.gene96331 | -0.811034 | 8.57E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene96331 | -0.862198 | 6.00E-64 | -1.69E-46 |
| MS.gene051983 | MS.gene96331 | -0.855103 | 7.90E-62 | -1.69E-46 |
| MS.gene052077 | MS.gene96331 | -0.839396 | 1.62E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene96331 | -0.848233 | 6.98E-60 | -1.69E-46 |
| MS.gene052115 | MS.gene96331 | -0.800712 | 1.27E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene96331 | -0.8348 | 2.43E-56 | -1.69E-46 |
| MS.gene052178 | MS.gene96331 | -0.884717 | 1.51E-71 | -1.69E-46 |
| MS.gene052188 | MS.gene96331 | -0.828753 | 7.55E-55 | -1.69E-46 |
| MS.gene052207 | MS.gene96331 | -0.826396 | 2.78E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene96331 | -0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene96331 | -0.814514 | 1.48E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene96331 | 0.868676 | 5.47E-66 | -1.69E-46 |
| MS.gene052310 | MS.gene96331 | -0.803901 | 2.79E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene96331 | -0.8461 | 2.69E-59 | -1.69E-46 |
| MS.gene052344 | MS.gene96331 | 0.827195 | 1.79E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene96331 | -0.814537 | 1.46E-51 | -1.69E-46 |
| MS.gene052600 | MS.gene96331 | -0.840787 | 7.04E-58 | -1.69E-46 |
| MS.gene052917 | MS.gene96331 | 0.81934 | 1.22E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene96331 | -0.806792 | 6.93E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene96331 | -0.850582 | 1.55E-60 | -1.69E-46 |
| MS.gene053836 | MS.gene96331 | -0.904342 | 1.36E-79 | -1.69E-46 |
| MS.gene053907 | MS.gene96331 | -0.828369 | 9.34E-55 | -1.69E-46 |
| MS.gene053971 | MS.gene96331 | -0.814304 | 1.65E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene96331 | -0.81089 | 9.21E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene96331 | -0.800183 | 1.62E-48 | -1.69E-46 |
| MS.gene054184 | MS.gene96331 | -0.833071 | 6.58E-56 | -1.69E-46 |
| MS.gene054185 | MS.gene96331 | -0.834122 | 3.60E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene96331 | -0.801381 | 9.25E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene96331 | -0.806793 | 6.92E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene96331 | -0.849211 | 3.74E-60 | -1.69E-46 |
| MS.gene054542 | MS.gene96331 | -0.828095 | 1.09E-54 | -1.69E-46 |
| MS.gene054667 | MS.gene96331 | -0.823345 | 1.46E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene96331 | -0.856469 | 3.15E-62 | -1.69E-46 |
| MS.gene054942 | MS.gene96331 | -0.866397 | 2.94E-65 | -1.69E-46 |
| MS.gene054983 | MS.gene96331 | -0.840876 | 6.68E-58 | -1.69E-46 |
| MS.gene055133 | MS.gene96331 | -0.818927 | 1.52E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene96331 | -0.868509 | 6.19E-66 | -1.69E-46 |
| MS.gene055390 | MS.gene96331 | -0.837134 | 6.22E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene96331 | -0.884973 | 1.21E-71 | -1.69E-46 |
| MS.gene055428 | MS.gene96331 | -0.868891 | 4.66E-66 | -1.69E-46 |
| MS.gene05553 | MS.gene96331 | -0.812544 | 4.02E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene96331 | -0.845238 | 4.60E-59 | -1.69E-46 |
| MS.gene055712 | MS.gene96331 | -0.832838 | 7.52E-56 | -1.69E-46 |
| MS.gene055859 | MS.gene96331 | -0.814237 | 1.71E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene96331 | 0.888968 | 3.69E-73 | -1.69E-46 |
| MS.gene056314 | MS.gene96331 | -0.828128 | 1.07E-54 | -1.69E-46 |
| MS.gene056612 | MS.gene96331 | -0.85878 | 6.51E-63 | -1.69E-46 |
| MS.gene056772 | MS.gene96331 | -0.825113 | 5.59E-54 | -1.69E-46 |
| MS.gene056875 | MS.gene96331 | -0.825902 | 3.64E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene96331 | -0.845999 | 2.86E-59 | -1.69E-46 |
| MS.gene05702 | MS.gene96331 | -0.83635 | 9.85E-57 | -1.69E-46 |
| MS.gene057121 | MS.gene96331 | -0.855689 | 5.33E-62 | -1.69E-46 |
| MS.gene057245 | MS.gene96331 | -0.835255 | 1.87E-56 | -1.69E-46 |
| MS.gene057260 | MS.gene96331 | -0.847657 | 1.01E-59 | -1.69E-46 |
| MS.gene057439 | MS.gene96331 | -0.87998 | 8.00E-70 | -1.69E-46 |
| MS.gene057448 | MS.gene96331 | -0.80413 | 2.50E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene96331 | -0.812691 | 3.73E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene96331 | -0.813178 | 2.92E-51 | -1.69E-46 |
| MS.gene057754 | MS.gene96331 | -0.816131 | 6.48E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene96331 | -0.801738 | 7.81E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene96331 | -0.870582 | 1.31E-66 | -1.69E-46 |
| MS.gene058082 | MS.gene96331 | -0.830408 | 2.99E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene96331 | -0.888261 | 6.91E-73 | -1.69E-46 |
| MS.gene058139 | MS.gene96331 | -0.803989 | 2.68E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene96331 | -0.856728 | 2.64E-62 | -1.69E-46 |
| MS.gene058182 | MS.gene96331 | -0.831948 | 1.25E-55 | -1.69E-46 |
| MS.gene058201 | MS.gene96331 | -0.828602 | 8.21E-55 | -1.69E-46 |
| MS.gene058233 | MS.gene96331 | -0.806871 | 6.67E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene96331 | -0.826036 | 3.38E-54 | -1.69E-46 |
| MS.gene058399 | MS.gene96331 | -0.802278 | 6.05E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene96331 | -0.807542 | 4.80E-50 | -1.69E-46 |
| MS.gene058661 | MS.gene96331 | 0.818497 | 1.90E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene96331 | -0.843707 | 1.19E-58 | -1.69E-46 |
| MS.gene058749 | MS.gene96331 | -0.849996 | 2.26E-60 | -1.69E-46 |
| MS.gene058833 | MS.gene96331 | -0.841243 | 5.35E-58 | -1.69E-46 |
| MS.gene058845 | MS.gene96331 | -0.856487 | 3.11E-62 | -1.69E-46 |
| MS.gene059033 | MS.gene96331 | 0.806523 | 7.89E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene96331 | -0.846643 | 1.91E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene96331 | -0.820395 | 7.02E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene96331 | -0.816267 | 6.04E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene96331 | -0.880534 | 5.07E-70 | -1.69E-46 |
| MS.gene059597 | MS.gene96331 | -0.843409 | 1.43E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene96331 | -0.802822 | 4.67E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene96331 | -0.814576 | 1.44E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene96331 | -0.812321 | 4.50E-51 | -1.69E-46 |
| MS.gene059708 | MS.gene96331 | -0.807981 | 3.88E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene96331 | -0.901404 | 2.77E-78 | -1.69E-46 |
| MS.gene059894 | MS.gene96331 | -0.883591 | 3.94E-71 | -1.69E-46 |
| MS.gene059899 | MS.gene96331 | -0.857598 | 1.46E-62 | -1.69E-46 |
| MS.gene060084 | MS.gene96331 | -0.821855 | 3.23E-53 | -1.69E-46 |
| MS.gene060165 | MS.gene96331 | -0.814634 | 1.39E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene96331 | -0.841912 | 3.56E-58 | -1.69E-46 |
| MS.gene060407 | MS.gene96331 | -0.815303 | 9.91E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene96331 | -0.882374 | 1.10E-70 | -1.69E-46 |
| MS.gene060619 | MS.gene96331 | -0.813939 | 1.99E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene96331 | -0.884416 | 1.95E-71 | -1.69E-46 |
| MS.gene061112 | MS.gene96331 | -0.805166 | 1.52E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene96331 | -0.803764 | 2.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96331.t1 | MTR_4g127040 | 100.000 | 108 | 0 | 0 | 56 | 163 | 157 | 264 | 7.45e-74 | 224 |
| MS.gene96331.t1 | MTR_4g127040 | 94.444 | 36 | 2 | 0 | 1 | 36 | 1 | 36 | 2.02e-15 | 72.4 |
| MS.gene96331.t1 | MTR_1g009613 | 56.934 | 137 | 39 | 5 | 46 | 163 | 146 | 281 | 2.99e-36 | 128 |
| MS.gene96331.t1 | MTR_3g108960 | 58.036 | 112 | 36 | 5 | 62 | 163 | 223 | 333 | 8.86e-33 | 120 |
| MS.gene96331.t1 | MTR_4g094908 | 48.361 | 122 | 47 | 4 | 58 | 163 | 164 | 285 | 2.61e-27 | 104 |
| MS.gene96331.t1 | MTR_5g016610 | 75.472 | 53 | 13 | 0 | 111 | 163 | 166 | 218 | 2.06e-23 | 93.2 |
| MS.gene96331.t1 | MTR_1g070455 | 49.524 | 105 | 42 | 4 | 67 | 163 | 211 | 312 | 1.18e-19 | 84.3 |
| MS.gene96331.t1 | MTR_0117s0040 | 63.415 | 41 | 15 | 0 | 123 | 163 | 53 | 93 | 3.96e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96331.t1 | AT4G31550 | 59.524 | 126 | 37 | 4 | 52 | 163 | 140 | 265 | 1.07e-41 | 142 |
| MS.gene96331.t1 | AT4G31550 | 59.524 | 126 | 36 | 5 | 52 | 163 | 140 | 264 | 3.62e-41 | 139 |
| MS.gene96331.t1 | AT4G31550 | 59.524 | 126 | 36 | 5 | 52 | 163 | 140 | 264 | 1.47e-40 | 139 |
| MS.gene96331.t1 | AT2G24570 | 60.185 | 108 | 28 | 4 | 71 | 163 | 155 | 262 | 6.43e-35 | 124 |
| MS.gene96331.t1 | AT4G24240 | 70.513 | 78 | 15 | 2 | 87 | 163 | 230 | 300 | 1.60e-29 | 109 |
| MS.gene96331.t1 | AT4G24240 | 70.513 | 78 | 15 | 2 | 87 | 163 | 230 | 300 | 4.07e-29 | 109 |
| MS.gene96331.t1 | AT5G28650 | 52.336 | 107 | 49 | 1 | 59 | 163 | 175 | 281 | 1.51e-27 | 105 |
| MS.gene96331.t1 | AT2G30590 | 51.351 | 111 | 43 | 3 | 62 | 163 | 224 | 332 | 2.90e-26 | 102 |
| MS.gene96331.t1 | AT3G04670 | 39.286 | 168 | 88 | 4 | 5 | 163 | 119 | 281 | 5.25e-26 | 100 |
| MS.gene96331.t1 | AT3G04670 | 39.286 | 168 | 88 | 4 | 5 | 163 | 119 | 281 | 1.26e-25 | 100 |
| MS.gene96331.t1 | AT2G23320 | 57.333 | 75 | 28 | 2 | 93 | 163 | 185 | 259 | 3.53e-24 | 95.1 |
| MS.gene96331.t1 | AT2G23320 | 57.333 | 75 | 28 | 2 | 93 | 163 | 185 | 259 | 1.30e-23 | 94.7 |
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCATCCATCCTCATCTT+TGG | 0.260460 | 8.4:-7782454 | None:intergenic |
| GGGTAAGGTGAACCTTTAAT+AGG | 0.321141 | 8.4:-7783293 | None:intergenic |
| ATTCAAGAAGCTGCTTCTGC+TGG | 0.410708 | 8.4:+7782484 | MS.gene96331:CDS |
| ATCGGAAACGCTTCCATCTC+CGG | 0.412134 | 8.4:-7782950 | None:intergenic |
| TTCTGCTGGATTGAAAAGTA+TGG | 0.429072 | 8.4:+7782498 | MS.gene96331:CDS |
| GAAGAGAACAATTCGTGTTC+CGG | 0.431711 | 8.4:+7783205 | MS.gene96331:CDS |
| TTGCTTCATCTCACCGGAAA+AGG | 0.439863 | 8.4:+7783013 | MS.gene96331:CDS |
| AGCGTTTCCGATGGGAAAAT+CGG | 0.462137 | 8.4:+7782961 | MS.gene96331:CDS |
| TGTCACGAAGCCACCATCTC+CGG | 0.476339 | 8.4:+7783036 | MS.gene96331:CDS |
| TCAACGTAACCAACTAGATC+TGG | 0.482987 | 8.4:+7782573 | MS.gene96331:CDS |
| AGACAAGATGGTTGTAGATC+CGG | 0.492204 | 8.4:+7782423 | None:intergenic |
| ACCGGAGGATGAGACTTTGC+CGG | 0.493124 | 8.4:-7783055 | None:intergenic |
| ATAGAAAATTACCGTGGGTA+AGG | 0.495916 | 8.4:-7783308 | None:intergenic |
| GTTTATTAACCAGATCTAGT+TGG | 0.498964 | 8.4:-7782582 | None:intergenic |
| GGATGAGACTTTGCCGGAGA+TGG | 0.499465 | 8.4:-7783049 | None:intergenic |
| TCCGGCAAAGTCTCATCCTC+CGG | 0.500468 | 8.4:+7783054 | MS.gene96331:CDS |
| CCCCTCTTGCTTCATCTCAC+CGG | 0.500785 | 8.4:+7783007 | MS.gene96331:CDS |
| CTCCACGAAAATTCGTCTGA+TGG | 0.502658 | 8.4:-7783254 | None:intergenic |
| AACGCTTCCATCTCCGGTGA+TGG | 0.507110 | 8.4:-7782944 | None:intergenic |
| ATCGGTCCTATCATTTCCTC+CGG | 0.509860 | 8.4:+7782979 | MS.gene96331:CDS |
| CTCATCTTTGGAATCACTAC+CGG | 0.510127 | 8.4:-7782442 | None:intergenic |
| ATGTCCACCATCACCGGAGA+TGG | 0.512485 | 8.4:+7782937 | MS.gene96331:CDS |
| TCTTTCATGTCCACCATCAC+CGG | 0.515159 | 8.4:+7782931 | MS.gene96331:CDS |
| CTTAGAGCAATGGCAGTGAC+CGG | 0.532910 | 8.4:-7783073 | None:intergenic |
| TTTACCTTCTCTTAGAGCAA+TGG | 0.555901 | 8.4:-7783083 | None:intergenic |
| GCTTCCATCTCCGGTGATGG+TGG | 0.558489 | 8.4:-7782941 | None:intergenic |
| TCCGGTGAGATGAAGCAAGA+GGG | 0.562916 | 8.4:-7783008 | None:intergenic |
| TGAGACTTTGCCGGAGATGG+TGG | 0.571854 | 8.4:-7783046 | None:intergenic |
| GGAGATGGAAGCGTTTCCGA+TGG | 0.600674 | 8.4:+7782952 | MS.gene96331:CDS |
| GGCTTGCCGGAGGAAATGAT+AGG | 0.601533 | 8.4:-7782985 | None:intergenic |
| CGGTAGTGATTCCAAAGATG+AGG | 0.606458 | 8.4:+7782443 | MS.gene96331:CDS |
| ACTGCCATTGCTCTAAGAGA+AGG | 0.610372 | 8.4:+7783079 | MS.gene96331:CDS |
| AGTGATTCCAAAGATGAGGA+TGG | 0.614432 | 8.4:+7782447 | MS.gene96331:CDS |
| GAGATGGAAGCGTTTCCGAT+GGG | 0.631567 | 8.4:+7782953 | MS.gene96331:CDS |
| TTCCGGTGAGATGAAGCAAG+AGG | 0.637613 | 8.4:-7783009 | None:intergenic |
| CGGTGAGATGAAGCAAGAGG+GGG | 0.640070 | 8.4:-7783006 | None:intergenic |
| CCGGTGAGATGAAGCAAGAG+GGG | 0.640954 | 8.4:-7783007 | None:intergenic |
| TTAAAGGTTCACCTTACCCA+CGG | 0.642405 | 8.4:+7783297 | MS.gene96331:CDS |
| GGACATGAAAGACGAAGTAG+TGG | 0.653461 | 8.4:-7782920 | None:intergenic |
| AGAGCAATGGCAGTGACCGG+AGG | 0.667641 | 8.4:-7783070 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAATTTAGTGTTTGGATTT+GGG | - | chr8.4:7783111-7783130 | None:intergenic | 25.0% |
| ! | TTACTTGAGAATTTAGTGTT+TGG | - | chr8.4:7783118-7783137 | None:intergenic | 25.0% |
| !!! | ATTAACTTGTTAAATCGTAC+CGG | + | chr8.4:7782655-7782674 | MS.gene96331:intron | 25.0% |
| !!! | ATTAGTTTTAGGTTTGAGAT+TGG | - | chr8.4:7782839-7782858 | None:intergenic | 25.0% |
| !!! | CACTGTTTCTAAGTTTAAAA+CGG | + | chr8.4:7782630-7782649 | MS.gene96331:intron | 25.0% |
| !!! | CTTGTTGTTTTTTCTTAATC+AGG | + | chr8.4:7783171-7783190 | MS.gene96331:intron | 25.0% |
| !!! | TCAGTAGATTGATTAGTTTT+AGG | - | chr8.4:7782850-7782869 | None:intergenic | 25.0% |
| !!! | TTAGTTTTAGGTTTGAGATT+GGG | - | chr8.4:7782838-7782857 | None:intergenic | 25.0% |
| GAGAATTTAGTGTTTGGATT+TGG | - | chr8.4:7783112-7783131 | None:intergenic | 30.0% | |
| GTTTATTAACCAGATCTAGT+TGG | - | chr8.4:7782585-7782604 | None:intergenic | 30.0% | |
| TACGGTCAAAAACCTATTAA+AGG | + | chr8.4:7783281-7783300 | MS.gene96331:CDS | 30.0% | |
| TGAAATTGAGAAGAAGAAGA+AGG | - | chr8.4:7782703-7782722 | None:intergenic | 30.0% | |
| TTCTCAATACTCCAAATCAA+AGG | + | chr8.4:7782879-7782898 | MS.gene96331:intron | 30.0% | |
| TTGGATTTGACATGACTAAT+TGG | - | chr8.4:7782795-7782814 | None:intergenic | 30.0% | |
| TTTGACATGACTAATTGGAT+CGG | - | chr8.4:7782790-7782809 | None:intergenic | 30.0% | |
| TTTGGATTTGGATTTGGATT+TGG | - | chr8.4:7782808-7782827 | None:intergenic | 30.0% | |
| AAATCAAGAGTAGTTGACTG+TGG | - | chr8.4:7782760-7782779 | None:intergenic | 35.0% | |
| GCTATAAGCTTCCTTTGATT+TGG | - | chr8.4:7782893-7782912 | None:intergenic | 35.0% | |
| GGTTGAATCTTGAATTTCTC+AGG | - | chr8.4:7782739-7782758 | None:intergenic | 35.0% | |
| TTCTGCTGGATTGAAAAGTA+TGG | + | chr8.4:7782498-7782517 | MS.gene96331:CDS | 35.0% | |
| TTGAGATTGGGATTTGGATT+TGG | - | chr8.4:7782826-7782845 | None:intergenic | 35.0% | |
| TTGGGATTTGGATTTGGATT+TGG | - | chr8.4:7782820-7782839 | None:intergenic | 35.0% | |
| TTTACCTTCTCTTAGAGCAA+TGG | - | chr8.4:7783086-7783105 | None:intergenic | 35.0% | |
| ! | GAATTTTCGTGGAGGAAATA+CGG | + | chr8.4:7783263-7783282 | MS.gene96331:CDS | 35.0% |
| ! | TTAGGTTTGAGATTGGGATT+TGG | - | chr8.4:7782832-7782851 | None:intergenic | 35.0% |
| AGTGATTCCAAAGATGAGGA+TGG | + | chr8.4:7782447-7782466 | MS.gene96331:CDS | 40.0% | |
| GAAGAGAACAATTCGTGTTC+CGG | + | chr8.4:7783205-7783224 | MS.gene96331:CDS | 40.0% | |
| GGGTAAGGTGAACCTTTAAT+AGG | - | chr8.4:7783296-7783315 | None:intergenic | 40.0% | |
| TCAACGTAACCAACTAGATC+TGG | + | chr8.4:7782573-7782592 | MS.gene96331:CDS | 40.0% | |
| TGTTCATCCATCCTCATCTT+TGG | - | chr8.4:7782457-7782476 | None:intergenic | 40.0% | |
| ! | TTCCATCAGACGAATTTTCG+TGG | + | chr8.4:7783252-7783271 | MS.gene96331:CDS | 40.0% |
| !! | CTCATCTTTGGAATCACTAC+CGG | - | chr8.4:7782445-7782464 | None:intergenic | 40.0% |
| AGCGTTTCCGATGGGAAAAT+CGG | + | chr8.4:7782961-7782980 | MS.gene96331:CDS | 45.0% | |
| ATCGGTCCTATCATTTCCTC+CGG | + | chr8.4:7782979-7782998 | MS.gene96331:CDS | 45.0% | |
| ATTCAAGAAGCTGCTTCTGC+TGG | + | chr8.4:7782484-7782503 | MS.gene96331:CDS | 45.0% | |
| CGGTAGTGATTCCAAAGATG+AGG | + | chr8.4:7782443-7782462 | MS.gene96331:CDS | 45.0% | |
| CTCCACGAAAATTCGTCTGA+TGG | - | chr8.4:7783257-7783276 | None:intergenic | 45.0% | |
| GGACATGAAAGACGAAGTAG+TGG | - | chr8.4:7782923-7782942 | None:intergenic | 45.0% | |
| TCTTTCATGTCCACCATCAC+CGG | + | chr8.4:7782931-7782950 | MS.gene96331:CDS | 45.0% | |
| TTGCTTCATCTCACCGGAAA+AGG | + | chr8.4:7783013-7783032 | MS.gene96331:CDS | 45.0% | |
| ! | CATCAGACGAATTTTCGTGG+AGG | + | chr8.4:7783255-7783274 | MS.gene96331:CDS | 45.0% |
| ! | GCGACTTTTGAACTTATCGC+CGG | - | chr8.4:7783227-7783246 | None:intergenic | 45.0% |
| !! | ACTGCCATTGCTCTAAGAGA+AGG | + | chr8.4:7783079-7783098 | MS.gene96331:CDS | 45.0% |
| !! | GATAGGACCGATTTTCCCAT+CGG | - | chr8.4:7782971-7782990 | None:intergenic | 45.0% |
| ACGACGAAAACGAGCATGAC+CGG | - | chr8.4:7782677-7782696 | None:intergenic | 50.0% | |
| ATCGGAAACGCTTCCATCTC+CGG | - | chr8.4:7782953-7782972 | None:intergenic | 50.0% | |
| CTTAGAGCAATGGCAGTGAC+CGG | - | chr8.4:7783076-7783095 | None:intergenic | 50.0% | |
| TCCGGTGAGATGAAGCAAGA+GGG | - | chr8.4:7783011-7783030 | None:intergenic | 50.0% | |
| TTCCGGTGAGATGAAGCAAG+AGG | - | chr8.4:7783012-7783031 | None:intergenic | 50.0% | |
| ! | TGGCTTCGTGACACCTTTTC+CGG | - | chr8.4:7783029-7783048 | None:intergenic | 50.0% |
| !! | GAGATGGAAGCGTTTCCGAT+GGG | + | chr8.4:7782953-7782972 | MS.gene96331:CDS | 50.0% |
| AACGCTTCCATCTCCGGTGA+TGG | - | chr8.4:7782947-7782966 | None:intergenic | 55.0% | |
| ACCGGAGGATGAGACTTTGC+CGG | - | chr8.4:7783058-7783077 | None:intergenic | 55.0% | |
| ATGTCCACCATCACCGGAGA+TGG | + | chr8.4:7782937-7782956 | MS.gene96331:CDS | 55.0% | |
| CCCCTCTTGCTTCATCTCAC+CGG | + | chr8.4:7783007-7783026 | MS.gene96331:CDS | 55.0% | |
| CCGGTGAGATGAAGCAAGAG+GGG | - | chr8.4:7783010-7783029 | None:intergenic | 55.0% | |
| CGGTGAGATGAAGCAAGAGG+GGG | - | chr8.4:7783009-7783028 | None:intergenic | 55.0% | |
| GGATGAGACTTTGCCGGAGA+TGG | - | chr8.4:7783052-7783071 | None:intergenic | 55.0% | |
| GGCTTGCCGGAGGAAATGAT+AGG | - | chr8.4:7782988-7783007 | None:intergenic | 55.0% | |
| TCCGGCAAAGTCTCATCCTC+CGG | + | chr8.4:7783054-7783073 | MS.gene96331:CDS | 55.0% | |
| TGAGACTTTGCCGGAGATGG+TGG | - | chr8.4:7783049-7783068 | None:intergenic | 55.0% | |
| TGTCACGAAGCCACCATCTC+CGG | + | chr8.4:7783036-7783055 | MS.gene96331:CDS | 55.0% | |
| !! | GGAGATGGAAGCGTTTCCGA+TGG | + | chr8.4:7782952-7782971 | MS.gene96331:CDS | 55.0% |
| AGAGCAATGGCAGTGACCGG+AGG | - | chr8.4:7783073-7783092 | None:intergenic | 60.0% | |
| TGAAGCAAGAGGGGGCTTGC+CGG | - | chr8.4:7783001-7783020 | None:intergenic | 60.0% | |
| ! | GCTTCCATCTCCGGTGATGG+TGG | - | chr8.4:7782944-7782963 | None:intergenic | 60.0% |
| AGCAAGAGGGGGCTTGCCGG+AGG | - | chr8.4:7782998-7783017 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 7782430 | 7783318 | 7782430 | ID=MS.gene96331 |
| chr8.4 | mRNA | 7782430 | 7783318 | 7782430 | ID=MS.gene96331.t1;Parent=MS.gene96331 |
| chr8.4 | exon | 7782430 | 7782594 | 7782430 | ID=MS.gene96331.t1.exon1;Parent=MS.gene96331.t1 |
| chr8.4 | CDS | 7782430 | 7782594 | 7782430 | ID=cds.MS.gene96331.t1;Parent=MS.gene96331.t1 |
| chr8.4 | exon | 7782901 | 7783100 | 7782901 | ID=MS.gene96331.t1.exon2;Parent=MS.gene96331.t1 |
| chr8.4 | CDS | 7782901 | 7783100 | 7782901 | ID=cds.MS.gene96331.t1;Parent=MS.gene96331.t1 |
| chr8.4 | exon | 7783193 | 7783318 | 7783193 | ID=MS.gene96331.t1.exon3;Parent=MS.gene96331.t1 |
| chr8.4 | CDS | 7783193 | 7783318 | 7783193 | ID=cds.MS.gene96331.t1;Parent=MS.gene96331.t1 |
| Gene Sequence |
| Protein sequence |
