AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021732.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021732.01.T01 MTR_4g074150 83.582 67 11 0 25 91 125 191 3.06e-35 129
MsG0480021732.01.T01 MTR_4g074480 79.661 59 8 1 108 162 8 66 2.85e-20 84.7
MsG0480021732.01.T01 MTR_4g074480 79.661 59 8 1 108 162 23 81 4.75e-20 84.3
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 8 66 5.31e-20 84.3
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 38 96 6.43e-20 84.3
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 23 81 7.10e-20 84.3
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 8 66 8.35e-20 85.1
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 23 81 1.04e-19 85.1
MsG0480021732.01.T01 MTR_4g108000 79.661 59 8 1 108 162 23 81 1.11e-19 85.1
MsG0480021732.01.T01 MTR_4g113240 35.833 120 66 4 4 113 16 134 8.22e-14 68.6
MsG0480021732.01.T01 MTR_3g098280 51.667 60 29 0 32 91 103 162 3.10e-13 66.6
MsG0480021732.01.T01 MTR_7g083820 34.409 93 58 2 1 91 80 171 6.47e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021732.01.T01 AT1G50270 34.375 128 72 3 2 129 106 221 3.26e-15 72.4

Find 38 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGAGCATGAAACATAAA+AGG 0.252581 4:+63673819 None:intergenic
ATTGATTTGATTTCGGCTTT+TGG 0.257426 4:-63673759 MsG0480021732.01.T01:CDS
CTTGGACAGCTTTGATTAAT+GGG 0.293097 4:-63673671 MsG0480021732.01.T01:CDS
TGCTTGTATTGATTTGATTT+CGG 0.312886 4:-63673766 MsG0480021732.01.T01:CDS
GCTTGGACAGCTTTGATTAA+TGG 0.334007 4:-63673672 MsG0480021732.01.T01:CDS
GTGAATTGAGAATGAGAAAA+TGG 0.354008 4:+63673879 None:intergenic
CATGCTCAAATATTTAAACT+TGG 0.381893 4:-63673807 MsG0480021732.01.T01:CDS
GGTTGAGAAATTGGATCCTC+TGG 0.453715 4:-63673300 MsG0480021732.01.T01:CDS
AGGGCCATCACCAATGGAAC+GGG 0.470664 4:+63673839 None:intergenic
TTTGATGCGATTAGAGAGCT+TGG 0.471851 4:-63673332 MsG0480021732.01.T01:CDS
ATTAGAGAGCTTGGGATTGA+GGG 0.476921 4:-63673323 MsG0480021732.01.T01:CDS
GATTAGAGAGCTTGGGATTG+AGG 0.480462 4:-63673324 MsG0480021732.01.T01:CDS
ATAATATGCACAATAATTGA+AGG 0.482405 4:+63673239 None:intergenic
ATGCTCAAATATTTAAACTT+GGG 0.489930 4:-63673806 MsG0480021732.01.T01:CDS
TTTACGATAACCCGTTCCAT+TGG 0.491394 4:-63673849 MsG0480021732.01.T01:CDS
CTCAAAGGGATATTGTTGCT+TGG 0.497017 4:-63673689 MsG0480021732.01.T01:CDS
TTGATGCGATTAGAGAGCTT+GGG 0.504525 4:-63673331 MsG0480021732.01.T01:CDS
GCATCAAACACTTGACTGAC+AGG 0.506089 4:+63673347 None:intergenic
GTTTGATGAAAGTCCTCAAA+GGG 0.517824 4:-63673703 MsG0480021732.01.T01:CDS
TTTGAGCATGAAACATAAAA+GGG 0.517905 4:+63673820 None:intergenic
CAAGCAACAATATCCCTTTG+AGG 0.518086 4:+63673690 None:intergenic
TGGTGCTGTGTTGGCATGTA+CGG 0.519867 4:-63673384 MsG0480021732.01.T01:CDS
CAAATCACTTGTGCCTCACC+AGG 0.528710 4:+63673630 None:intergenic
ATTTGTGGATATGAGATTGA+AGG 0.530666 4:-63673612 MsG0480021732.01.T01:CDS
GGTGAGGCACAAGTGATTTG+TGG 0.535077 4:-63673627 MsG0480021732.01.T01:CDS
AAGATGTGGTGGTGCTGTGT+TGG 0.538012 4:-63673393 MsG0480021732.01.T01:CDS
CATAAAAGGGCCATCACCAA+TGG 0.568107 4:+63673833 None:intergenic
AAGGGCCATCACCAATGGAA+CGG 0.587689 4:+63673838 None:intergenic
ATGCACAATAATTGAAGGAG+AGG 0.593181 4:+63673244 None:intergenic
ATAACCCGTTCCATTGGTGA+TGG 0.602596 4:-63673843 MsG0480021732.01.T01:CDS
TGTTTGATGAAAGTCCTCAA+AGG 0.607230 4:-63673704 MsG0480021732.01.T01:CDS
TTTGTGGATATGAGATTGAA+GGG 0.608327 4:-63673611 MsG0480021732.01.T01:intron
AGAAAATGGATGAAAAGACA+TGG 0.620717 4:+63673893 None:intergenic
TAGAGAGCTTGGGATTGAGG+GGG 0.627540 4:-63673321 MsG0480021732.01.T01:CDS
TTAGAGAGCTTGGGATTGAG+GGG 0.630912 4:-63673322 MsG0480021732.01.T01:CDS
CACAGGATGCTGCAAAGATG+TGG 0.672845 4:-63673407 MsG0480021732.01.T01:intron
GATGCAGATGTAATAACCAG+AGG 0.692682 4:+63673284 None:intergenic
AGGATGCTGCAAAGATGTGG+TGG 0.699925 4:-63673404 MsG0480021732.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATGCACAATAATTGA+AGG + Chr4:63673887-63673906 None:intergenic 20.0%
!! ATGCTCAAATATTTAAACTT+GGG - Chr4:63673317-63673336 MsG0480021732.01.T01:CDS 20.0%
! ATTTGAGCATGAAACATAAA+AGG + Chr4:63673307-63673326 None:intergenic 25.0%
! CATGCTCAAATATTTAAACT+TGG - Chr4:63673316-63673335 MsG0480021732.01.T01:CDS 25.0%
! TTTGAGCATGAAACATAAAA+GGG + Chr4:63673306-63673325 None:intergenic 25.0%
!!! TGCTTGTATTGATTTGATTT+CGG - Chr4:63673357-63673376 MsG0480021732.01.T01:CDS 25.0%
ATTTGTGGATATGAGATTGA+AGG - Chr4:63673511-63673530 MsG0480021732.01.T01:intron 30.0%
CAATCGTTATTTATTGTCAC+AGG - Chr4:63673699-63673718 MsG0480021732.01.T01:CDS 30.0%
GTGAATTGAGAATGAGAAAA+TGG + Chr4:63673247-63673266 None:intergenic 30.0%
TTTGTGGATATGAGATTGAA+GGG - Chr4:63673512-63673531 MsG0480021732.01.T01:intron 30.0%
TTTGTTAAAAACGATGTTCC+TGG - Chr4:63673475-63673494 MsG0480021732.01.T01:intron 30.0%
!! TTGATTTGATTTCGGCTTTT+GGG - Chr4:63673365-63673384 MsG0480021732.01.T01:CDS 30.0%
!! TTGTGATTGATGGTTTTCTA+TGG - Chr4:63673537-63673556 MsG0480021732.01.T01:intron 30.0%
!!! ATTGATTTGATTTCGGCTTT+TGG - Chr4:63673364-63673383 MsG0480021732.01.T01:CDS 30.0%
AAAAAAACCAAACGCTTTGC+AGG + Chr4:63673619-63673638 None:intergenic 35.0%
ATGCACAATAATTGAAGGAG+AGG + Chr4:63673882-63673901 None:intergenic 35.0%
GTTTGATGAAAGTCCTCAAA+GGG - Chr4:63673420-63673439 MsG0480021732.01.T01:intron 35.0%
TGTTTGATGAAAGTCCTCAA+AGG - Chr4:63673419-63673438 MsG0480021732.01.T01:intron 35.0%
TTCTGTTTATTGTGCTCTTG+TGG - Chr4:63673560-63673579 MsG0480021732.01.T01:intron 35.0%
! CTTGGACAGCTTTGATTAAT+GGG - Chr4:63673452-63673471 MsG0480021732.01.T01:intron 35.0%
ATTAGAGAGCTTGGGATTGA+GGG - Chr4:63673800-63673819 MsG0480021732.01.T01:CDS 40.0%
CAAGCAACAATATCCCTTTG+AGG + Chr4:63673436-63673455 None:intergenic 40.0%
CTCAAAGGGATATTGTTGCT+TGG - Chr4:63673434-63673453 MsG0480021732.01.T01:intron 40.0%
GATGCAGATGTAATAACCAG+AGG + Chr4:63673842-63673861 None:intergenic 40.0%
TAAAAACGATGTTCCTGGTG+AGG - Chr4:63673480-63673499 MsG0480021732.01.T01:intron 40.0%
TTGATGCGATTAGAGAGCTT+GGG - Chr4:63673792-63673811 MsG0480021732.01.T01:CDS 40.0%
TTTACGATAACCCGTTCCAT+TGG - Chr4:63673274-63673293 MsG0480021732.01.T01:CDS 40.0%
TTTGATGCGATTAGAGAGCT+TGG - Chr4:63673791-63673810 MsG0480021732.01.T01:CDS 40.0%
! GCTTGGACAGCTTTGATTAA+TGG - Chr4:63673451-63673470 MsG0480021732.01.T01:intron 40.0%
! GTGGACATGTATTTCAAGTG+TGG - Chr4:63673579-63673598 MsG0480021732.01.T01:intron 40.0%
!! TTGAAGGGTGTTGTGATTGA+TGG - Chr4:63673527-63673546 MsG0480021732.01.T01:intron 40.0%
ATAACCCGTTCCATTGGTGA+TGG - Chr4:63673280-63673299 MsG0480021732.01.T01:CDS 45.0%
CATAAAAGGGCCATCACCAA+TGG + Chr4:63673293-63673312 None:intergenic 45.0%
GATTAGAGAGCTTGGGATTG+AGG - Chr4:63673799-63673818 MsG0480021732.01.T01:CDS 45.0%
GATTGAGGGGGTTGAGAAAT+TGG - Chr4:63673814-63673833 MsG0480021732.01.T01:CDS 45.0%
GCATCAAACACTTGACTGAC+AGG + Chr4:63673779-63673798 None:intergenic 45.0%
GGTTGAGAAATTGGATCCTC+TGG - Chr4:63673823-63673842 MsG0480021732.01.T01:CDS 45.0%
TTAGAGAGCTTGGGATTGAG+GGG - Chr4:63673801-63673820 MsG0480021732.01.T01:CDS 45.0%
!! ATTTCGGCTTTTGGGTGTTC+TGG - Chr4:63673373-63673392 MsG0480021732.01.T01:CDS 45.0%
!! TTTCGGCTTTTGGGTGTTCT+GGG - Chr4:63673374-63673393 MsG0480021732.01.T01:CDS 45.0%
AAGGGCCATCACCAATGGAA+CGG + Chr4:63673288-63673307 None:intergenic 50.0%
AGGATGCTGCAAAGATGTGG+TGG - Chr4:63673719-63673738 MsG0480021732.01.T01:CDS 50.0%
CAAATCACTTGTGCCTCACC+AGG + Chr4:63673496-63673515 None:intergenic 50.0%
CACAGGATGCTGCAAAGATG+TGG - Chr4:63673716-63673735 MsG0480021732.01.T01:CDS 50.0%
GGTGAGGCACAAGTGATTTG+TGG - Chr4:63673496-63673515 MsG0480021732.01.T01:intron 50.0%
TAGAGAGCTTGGGATTGAGG+GGG - Chr4:63673802-63673821 MsG0480021732.01.T01:CDS 50.0%
! GATGATGCCTGCAAAGCGTT+TGG - Chr4:63673609-63673628 MsG0480021732.01.T01:intron 50.0%
!! AAGATGTGGTGGTGCTGTGT+TGG - Chr4:63673730-63673749 MsG0480021732.01.T01:CDS 50.0%
!! TGGTGCTGTGTTGGCATGTA+CGG - Chr4:63673739-63673758 MsG0480021732.01.T01:CDS 50.0%
AGGGCCATCACCAATGGAAC+GGG + Chr4:63673287-63673306 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63673232 63673913 63673232 ID=MsG0480021732.01;Name=MsG0480021732.01
Chr4 mRNA 63673232 63673913 63673232 ID=MsG0480021732.01.T01;Parent=MsG0480021732.01;Name=MsG0480021732.01.T01;_AED=0.50;_eAED=0.80;_QI=0|0|0|1|1|1|2|0|164
Chr4 exon 63673612 63673913 63673612 ID=MsG0480021732.01.T01:exon:3545;Parent=MsG0480021732.01.T01
Chr4 exon 63673232 63673424 63673232 ID=MsG0480021732.01.T01:exon:3546;Parent=MsG0480021732.01.T01
Chr4 CDS 63673612 63673913 63673612 ID=MsG0480021732.01.T01:cds;Parent=MsG0480021732.01.T01
Chr4 CDS 63673232 63673424 63673232 ID=MsG0480021732.01.T01:cds;Parent=MsG0480021732.01.T01
Gene Sequence

>MsG0480021732.01.T01

ATGTCTTTTCATCCATTTTCTCATTCTCAATTCACAATTCGTTTTACGATAACCCGTTCCATTGGTGATGGCCCTTTTATGTTTCATGCTCAAATATTTAAACTTGGGTTTGATTTTGATTGTTTTGCTTGTATTGATTTGATTTCGGCTTTTGGGTGTTCTGGGTTTATGAAAAGTGCGTGTAAAGTGTTTGATGAAAGTCCTCAAAGGGATATTGTTGCTTGGACAGCTTTGATTAATGGGTTTGTTAAAAACGATGTTCCTGGTGAGGCACAAGTGATTTGTGGATATGAGATTGAAGGGATGCTGCAAAGATGTGGTGGTGCTGTGTTGGCATGTACGGATGAAGCAGTTCTTCCTGTCAGTCAAGTGTTTGATGCGATTAGAGAGCTTGGGATTGAGGGGGTTGAGAAATTGGATCCTCTGGTTATTACATCTGCATCATCACTCAGTAATTCTACCTCTCCTTCAATTATTGTGCATATTATTATGTGA

Protein sequence

>MsG0480021732.01.T01

MSFHPFSHSQFTIRFTITRSIGDGPFMFHAQIFKLGFDFDCFACIDLISAFGCSGFMKSACKVFDESPQRDIVAWTALINGFVKNDVPGEAQVICGYEIEGMLQRCGGAVLACTDEAVLPVSQVFDAIRELGIEGVEKLDPLVITSASSLSNSTSPSIIVHIIM*