AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027074.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027074.01.T01 MTR_6g472100 97.561 369 4 3 1 365 1 368 0.0 713
MsG0580027074.01.T01 MTR_6g046300 93.478 46 1 2 223 268 36 79 4.65e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027074.01.T01 AT3G25590 36.465 447 147 19 1 348 1 409 2.08e-54 185

Find 57 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGAGGTGGAGCTATATT+TGG 0.183362 5:-52762869 MsG0580027074.01.T01:CDS
GGAGAATTGGGAAACTATTT+AGG 0.222773 5:-52763006 MsG0580027074.01.T01:CDS
TAGAAGAAATCACTTCTTCT+TGG 0.257265 5:+52762719 None:intergenic
TTCTTCTATGAATATGAAAA+TGG 0.271087 5:-52762704 MsG0580027074.01.T01:CDS
GGTGGTGGTGGTGGAGGTGA+TGG 0.288439 5:-52762476 MsG0580027074.01.T01:CDS
GCAGATACACTCTCAGCTTT+TGG 0.291697 5:-52762539 MsG0580027074.01.T01:CDS
ATTTCAGCATTTGAATTCTA+AGG 0.291707 5:-52763116 MsG0580027074.01.T01:CDS
TTCTTCTTTCTCTTGCATCT+TGG 0.305233 5:+52763093 None:intergenic
TTAGAATTCAAATGCTGAAA+TGG 0.334316 5:+52763118 None:intergenic
TCAAATATCATTCAAGTTCA+AGG 0.341466 5:-52762373 MsG0580027074.01.T01:CDS
ATTTCTGCAGCTAGAAGTTC+AGG 0.351861 5:-52762800 MsG0580027074.01.T01:CDS
TAGAATTCAAATGCTGAAAT+GGG 0.399760 5:+52763119 None:intergenic
CATGATGCTGGTGGTGGTGG+TGG 0.415319 5:-52762488 MsG0580027074.01.T01:CDS
TTACTGTCTTAAAGAACGTT+TGG 0.432017 5:-52763293 MsG0580027074.01.T01:CDS
GGTGGTGGTGGAGGTGATGG+AGG 0.453840 5:-52762473 MsG0580027074.01.T01:CDS
ATCATCATGGAGGTGTTTCA+AGG 0.475029 5:-52762910 MsG0580027074.01.T01:CDS
TAGAAAGAGTACTGGTGTAA+TGG 0.476645 5:-52762681 MsG0580027074.01.T01:CDS
GCAAGAGAAAGAAGAAGAAG+AGG 0.479357 5:-52763086 MsG0580027074.01.T01:CDS
TTGATGGAAGCAGCTACAAT+AGG 0.481008 5:-52762655 MsG0580027074.01.T01:CDS
GAAAGAAGAAGAAGAGGTTG+TGG 0.494932 5:-52763080 MsG0580027074.01.T01:CDS
GGTGGTTTGATGTTAGAATC+TGG 0.497991 5:-52762770 MsG0580027074.01.T01:CDS
TGAATGAAAGAGGAATTAAG+AGG 0.498145 5:-52762433 MsG0580027074.01.T01:CDS
AACAGAGCATGAATTTCGAT+TGG 0.499202 5:+52763201 None:intergenic
ACACATGATGCTGGTGGTGG+TGG 0.503836 5:-52762491 MsG0580027074.01.T01:CDS
ACTGGTGTAATGGAAATTGA+TGG 0.506563 5:-52762671 MsG0580027074.01.T01:CDS
AAGCTTGTGTGTTCAGATTG+TGG 0.522398 5:-52763268 MsG0580027074.01.T01:CDS
AAAGTATTAAGGGTTGGAGA+TGG 0.524569 5:-52762400 MsG0580027074.01.T01:CDS
GAAAATGGTAGAAAGAGTAC+TGG 0.527029 5:-52762689 MsG0580027074.01.T01:CDS
AATAAGAAGAACAAGGATTG+TGG 0.528290 5:-52762980 MsG0580027074.01.T01:CDS
GAAGAAGGATGCTTCTTACA+TGG 0.543996 5:+52763340 None:intergenic
GTTTGATGTTAGAATCTGGT+AGG 0.549457 5:-52762766 MsG0580027074.01.T01:CDS
GAATGAAAGAGGAATTAAGA+GGG 0.561877 5:-52762432 MsG0580027074.01.T01:CDS
ATGACACATGATGCTGGTGG+TGG 0.566242 5:-52762494 MsG0580027074.01.T01:CDS
GCTAGAAGTTCAGGTGTGAA+TGG 0.567727 5:-52762791 MsG0580027074.01.T01:CDS
TTAGAATCTGGTAGGAGAAG+TGG 0.568091 5:-52762758 MsG0580027074.01.T01:CDS
AGATCTCTCTGCAGTTTCAG+AGG 0.571126 5:-52762884 MsG0580027074.01.T01:CDS
AAGGATTGTGGTAGAAGTGT+TGG 0.572137 5:-52762968 MsG0580027074.01.T01:CDS
TCGAAATTCATGCTCTGTTG+AGG 0.575005 5:-52763197 MsG0580027074.01.T01:CDS
GCTATATTTGGATCTGAAGA+TGG 0.581428 5:-52762857 MsG0580027074.01.T01:CDS
AATTTCATGACACATGATGC+TGG 0.583341 5:-52762500 MsG0580027074.01.T01:CDS
ATGTGGATGGTTGATCATCA+TGG 0.586021 5:-52762923 MsG0580027074.01.T01:CDS
TAGGAAGAATAAGAAGAACA+AGG 0.590362 5:-52762987 MsG0580027074.01.T01:CDS
AGGTTCACTATGAATGAAAG+AGG 0.598015 5:-52762443 MsG0580027074.01.T01:CDS
AATTCATGCTCTGTTGAGGT+AGG 0.600288 5:-52763193 MsG0580027074.01.T01:CDS
TTGATGAGAAGAATGAGATG+TGG 0.604022 5:-52762940 MsG0580027074.01.T01:CDS
GATTGTGGTAGAAGTGTTGG+TGG 0.608270 5:-52762965 MsG0580027074.01.T01:CDS
GAGGTGATGGAGGTTCATGT+AGG 0.609166 5:-52762463 MsG0580027074.01.T01:CDS
TCTCTCTGCAGTTTCAGAGG+TGG 0.611498 5:-52762881 MsG0580027074.01.T01:CDS
TCTGTTGAGGTAGGAAGTGT+TGG 0.616434 5:-52763184 MsG0580027074.01.T01:CDS
AGAAGTTCAGGTGTGAATGG+TGG 0.616507 5:-52762788 MsG0580027074.01.T01:CDS
TGAAGCTTATAGCAATGACA+TGG 0.617179 5:-52763392 MsG0580027074.01.T01:CDS
GTTCAGATTGTGGAGAACAA+AGG 0.625847 5:-52763258 MsG0580027074.01.T01:CDS
ATTGTGGTAGAAGTGTTGGT+GGG 0.637779 5:-52762964 MsG0580027074.01.T01:CDS
TGAGAAGAATGAGATGTGGA+TGG 0.655301 5:-52762936 MsG0580027074.01.T01:CDS
ATATTTGGATCTGAAGATGG+TGG 0.728625 5:-52762854 MsG0580027074.01.T01:CDS
TGGATGGTTGATCATCATGG+AGG 0.776999 5:-52762920 MsG0580027074.01.T01:CDS
TTCATGACACATGATGCTGG+TGG 0.799625 5:-52762497 MsG0580027074.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTCATCTGAATCAAAAA+TGG - Chr5:52763194-52763213 MsG0580027074.01.T01:CDS 20.0%
!! TTCTTCTATGAATATGAAAA+TGG - Chr5:52763051-52763070 MsG0580027074.01.T01:CDS 20.0%
!!! ATATAAAAAGACATGGTTTT+TGG - Chr5:52762728-52762747 MsG0580027074.01.T01:CDS 20.0%
!!! GAAAGAAGAAAAAGTATTAA+GGG - Chr5:52763345-52763364 MsG0580027074.01.T01:CDS 20.0%
! ATTTCAGCATTTGAATTCTA+AGG - Chr5:52762639-52762658 MsG0580027074.01.T01:CDS 25.0%
! TAGAATTCAAATGCTGAAAT+GGG + Chr5:52762639-52762658 None:intergenic 25.0%
! TTAGAATTCAAATGCTGAAA+TGG + Chr5:52762640-52762659 None:intergenic 25.0%
!! TCAAATATCATTCAAGTTCA+AGG - Chr5:52763382-52763401 MsG0580027074.01.T01:CDS 25.0%
!! TGTGTTGATATAAAAAGACA+TGG - Chr5:52762721-52762740 MsG0580027074.01.T01:CDS 25.0%
!!! GGAAAGAAGAAAAAGTATTA+AGG - Chr5:52763344-52763363 MsG0580027074.01.T01:CDS 25.0%
AATAAGAAGAACAAGGATTG+TGG - Chr5:52762775-52762794 MsG0580027074.01.T01:CDS 30.0%
GAATGAAAGAGGAATTAAGA+GGG - Chr5:52763323-52763342 MsG0580027074.01.T01:CDS 30.0%
TAGAAGAAATCACTTCTTCT+TGG + Chr5:52763039-52763058 None:intergenic 30.0%
TAGGAAGAATAAGAAGAACA+AGG - Chr5:52762768-52762787 MsG0580027074.01.T01:CDS 30.0%
TGAATGAAAGAGGAATTAAG+AGG - Chr5:52763322-52763341 MsG0580027074.01.T01:CDS 30.0%
TTACTGTCTTAAAGAACGTT+TGG - Chr5:52762462-52762481 MsG0580027074.01.T01:CDS 30.0%
! CTAAGAGAGAGTGATTTTAA+AGG - Chr5:52763135-52763154 MsG0580027074.01.T01:CDS 30.0%
!! GAAGAAAAAGTATTAAGGGT+TGG - Chr5:52763349-52763368 MsG0580027074.01.T01:CDS 30.0%
AACAGAGCATGAATTTCGAT+TGG + Chr5:52762557-52762576 None:intergenic 35.0%
AATTTCATGACACATGATGC+TGG - Chr5:52763255-52763274 MsG0580027074.01.T01:CDS 35.0%
AGGTTCACTATGAATGAAAG+AGG - Chr5:52763312-52763331 MsG0580027074.01.T01:CDS 35.0%
ATATTTGGATCTGAAGATGG+TGG - Chr5:52762901-52762920 MsG0580027074.01.T01:CDS 35.0%
GCTATATTTGGATCTGAAGA+TGG - Chr5:52762898-52762917 MsG0580027074.01.T01:CDS 35.0%
GGAGAATTGGGAAACTATTT+AGG - Chr5:52762749-52762768 MsG0580027074.01.T01:CDS 35.0%
GTTTGATGTTAGAATCTGGT+AGG - Chr5:52762989-52763008 MsG0580027074.01.T01:CDS 35.0%
TGAAGCTTATAGCAATGACA+TGG - Chr5:52762363-52762382 MsG0580027074.01.T01:CDS 35.0%
TTCTTCTTTCTCTTGCATCT+TGG + Chr5:52762665-52762684 None:intergenic 35.0%
TTGATGAGAAGAATGAGATG+TGG - Chr5:52762815-52762834 MsG0580027074.01.T01:CDS 35.0%
! AAAGTATTAAGGGTTGGAGA+TGG - Chr5:52763355-52763374 MsG0580027074.01.T01:CDS 35.0%
! ACTGGTGTAATGGAAATTGA+TGG - Chr5:52763084-52763103 MsG0580027074.01.T01:CDS 35.0%
! GAAAATGGTAGAAAGAGTAC+TGG - Chr5:52763066-52763085 MsG0580027074.01.T01:CDS 35.0%
!! AGACATGGTTTTTGGAGAAT+TGG - Chr5:52762736-52762755 MsG0580027074.01.T01:CDS 35.0%
!! AGAGAGTGATTTTAAAGGCA+TGG - Chr5:52763140-52763159 MsG0580027074.01.T01:CDS 35.0%
!! GACATGGTTTTTGGAGAATT+GGG - Chr5:52762737-52762756 MsG0580027074.01.T01:CDS 35.0%
!! TAGAAAGAGTACTGGTGTAA+TGG - Chr5:52763074-52763093 MsG0580027074.01.T01:CDS 35.0%
AAGCTTGTGTGTTCAGATTG+TGG - Chr5:52762487-52762506 MsG0580027074.01.T01:CDS 40.0%
AATTCATGCTCTGTTGAGGT+AGG - Chr5:52762562-52762581 MsG0580027074.01.T01:CDS 40.0%
ATGTGGATGGTTGATCATCA+TGG - Chr5:52762832-52762851 MsG0580027074.01.T01:CDS 40.0%
ATTTCTGCAGCTAGAAGTTC+AGG - Chr5:52762955-52762974 MsG0580027074.01.T01:CDS 40.0%
CATCCTTCTTCTTCTTCTTC+TGG - Chr5:52762427-52762446 MsG0580027074.01.T01:CDS 40.0%
GAAAGAAGAAGAAGAGGTTG+TGG - Chr5:52762675-52762694 MsG0580027074.01.T01:CDS 40.0%
GAAGAAGGATGCTTCTTACA+TGG + Chr5:52762418-52762437 None:intergenic 40.0%
GCAAGAGAAAGAAGAAGAAG+AGG - Chr5:52762669-52762688 MsG0580027074.01.T01:CDS 40.0%
GGTGGTTTGATGTTAGAATC+TGG - Chr5:52762985-52763004 MsG0580027074.01.T01:CDS 40.0%
GTTCAGATTGTGGAGAACAA+AGG - Chr5:52762497-52762516 MsG0580027074.01.T01:CDS 40.0%
TCGAAATTCATGCTCTGTTG+AGG - Chr5:52762558-52762577 MsG0580027074.01.T01:CDS 40.0%
TGAGAAGAATGAGATGTGGA+TGG - Chr5:52762819-52762838 MsG0580027074.01.T01:CDS 40.0%
TTAGAATCTGGTAGGAGAAG+TGG - Chr5:52762997-52763016 MsG0580027074.01.T01:CDS 40.0%
TTCAGAGGTGGAGCTATATT+TGG - Chr5:52762886-52762905 MsG0580027074.01.T01:CDS 40.0%
TTGATGGAAGCAGCTACAAT+AGG - Chr5:52763100-52763119 MsG0580027074.01.T01:CDS 40.0%
! AAGGATTGTGGTAGAAGTGT+TGG - Chr5:52762787-52762806 MsG0580027074.01.T01:CDS 40.0%
!! ATCATCATGGAGGTGTTTCA+AGG - Chr5:52762845-52762864 MsG0580027074.01.T01:CDS 40.0%
!! ATTGTGGTAGAAGTGTTGGT+GGG - Chr5:52762791-52762810 MsG0580027074.01.T01:CDS 40.0%
!! GGTGGTGATTCAGTTTTATC+AGG - Chr5:52762919-52762938 MsG0580027074.01.T01:CDS 40.0%
!! TCAGCTTTTGGAAGCATAAG+AGG - Chr5:52763228-52763247 MsG0580027074.01.T01:CDS 40.0%
AGAAGTTCAGGTGTGAATGG+TGG - Chr5:52762967-52762986 MsG0580027074.01.T01:CDS 45.0%
AGATCTCTCTGCAGTTTCAG+AGG - Chr5:52762871-52762890 MsG0580027074.01.T01:CDS 45.0%
CCTCCAGAAGAAGAAGAAGA+AGG + Chr5:52762433-52762452 None:intergenic 45.0%
CCTTCTTCTTCTTCTTCTGG+AGG - Chr5:52762430-52762449 MsG0580027074.01.T01:CDS 45.0%
GCTAGAAGTTCAGGTGTGAA+TGG - Chr5:52762964-52762983 MsG0580027074.01.T01:CDS 45.0%
TGGATGGTTGATCATCATGG+AGG - Chr5:52762835-52762854 MsG0580027074.01.T01:CDS 45.0%
TTCATGACACATGATGCTGG+TGG - Chr5:52763258-52763277 MsG0580027074.01.T01:CDS 45.0%
! GCAGATACACTCTCAGCTTT+TGG - Chr5:52763216-52763235 MsG0580027074.01.T01:CDS 45.0%
! TCTGTTGAGGTAGGAAGTGT+TGG - Chr5:52762571-52762590 MsG0580027074.01.T01:CDS 45.0%
!! GATTGTGGTAGAAGTGTTGG+TGG - Chr5:52762790-52762809 MsG0580027074.01.T01:CDS 45.0%
!! GCTTTTGGAAGCATAAGAGG+TGG - Chr5:52763231-52763250 MsG0580027074.01.T01:CDS 45.0%
ATGACACATGATGCTGGTGG+TGG - Chr5:52763261-52763280 MsG0580027074.01.T01:CDS 50.0%
TCTCTCTGCAGTTTCAGAGG+TGG - Chr5:52762874-52762893 MsG0580027074.01.T01:CDS 50.0%
!! GAGGTGATGGAGGTTCATGT+AGG - Chr5:52763292-52763311 MsG0580027074.01.T01:CDS 50.0%
!! ACACATGATGCTGGTGGTGG+TGG - Chr5:52763264-52763283 MsG0580027074.01.T01:CDS 55.0%
!! CATGATGCTGGTGGTGGTGG+TGG - Chr5:52763267-52763286 MsG0580027074.01.T01:CDS 60.0%
!! GATGCTGGTGGTGGTGGTGG+TGG - Chr5:52763270-52763289 MsG0580027074.01.T01:CDS 65.0%
!! GGTGGTGGTGGAGGTGATGG+AGG - Chr5:52763282-52763301 MsG0580027074.01.T01:CDS 65.0%
!! GGTGGTGGTGGTGGAGGTGA+TGG - Chr5:52763279-52763298 MsG0580027074.01.T01:CDS 65.0%
!! GCTGGTGGTGGTGGTGGTGG+AGG - Chr5:52763273-52763292 MsG0580027074.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 52762340 52763437 52762340 ID=MsG0580027074.01;Name=MsG0580027074.01
Chr5 mRNA 52762340 52763437 52762340 ID=MsG0580027074.01.T01;Parent=MsG0580027074.01;Name=MsG0580027074.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|365
Chr5 exon 52762340 52763437 52762340 ID=MsG0580027074.01.T01:exon:17364;Parent=MsG0580027074.01.T01
Chr5 CDS 52762340 52763437 52762340 ID=MsG0580027074.01.T01:cds;Parent=MsG0580027074.01.T01
Gene Sequence

>MsG0580027074.01.T01

ATGAAGGAGAAAAACAAAGGTGTTGAAGCTTATAGCAATGACATGGATTGCTACTATTCTACTTCTGATTTTCTTCCATGTAAGAAGCATCCTTCTTCTTCTTCTTCTGGAGGAATCTGTGCTTACTGTCTTAAAGAACGTTTGGTCAAGCTTGTGTGTTCAGATTGTGGAGAACAAAGGCTTTCTTCTTGTTCATGCTCTGATGAGATCACTTCCAATCGAAATTCATGCTCTGTTGAGGTAGGAAGTGTTGGAAGAGTCTCATTTCTTATTGAGAATGAAAAGAATGAGACAAACCCATTTCAGCATTTGAATTCTAAGGCCAAGATGCAAGAGAAAGAAGAAGAAGAGGTTGTGGTGCTTAAGAGAAGTAGTAGCAGTTGTGTTGATATAAAAAGACATGGTTTTTGGAGAATTGGGAAACTATTTAGGAAGAATAAGAAGAACAAGGATTGTGGTAGAAGTGTTGGTGGGTTTGATGAGAAGAATGAGATGTGGATGGTTGATCATCATGGAGGTGTTTCAAGGTCAAGATCTCTCTGCAGTTTCAGAGGTGGAGCTATATTTGGATCTGAAGATGGTGGTGATTCAGTTTTATCAGGTGCTAGAAGCTCTATTTCTGCAGCTAGAAGTTCAGGTGTGAATGGTGGTTTGATGTTAGAATCTGGTAGGAGAAGTGGTTACAGTGAAGCTGAACCAAGAAGAAGTGATTTCTTCTATGAATATGAAAATGGTAGAAAGAGTACTGGTGTAATGGAAATTGATGGAAGCAGCTACAATAGGCGTGTGTTTTCACTAAGAGAGAGTGATTTTAAAGGCATGGATGAGTCTAGTTTTATTGATTTGAAGCTTGATTATTCATCTGAATCAAAAATGGCAGATACACTCTCAGCTTTTGGAAGCATAAGAGGTGGAAATTTCATGACACATGATGCTGGTGGTGGTGGTGGTGGAGGTGATGGAGGTTCATGTAGGTTCACTATGAATGAAAGAGGAATTAAGAGGGAAAGAAGAAAAAGTATTAAGGGTTGGAGATGGATTTTCAAATATCATTCAAGTTCAAGGAAAAGAGATCAAGACCTTATGTTCAAAACCTAA

Protein sequence

>MsG0580027074.01.T01

MKEKNKGVEAYSNDMDCYYSTSDFLPCKKHPSSSSSGGICAYCLKERLVKLVCSDCGEQRLSSCSCSDEITSNRNSCSVEVGSVGRVSFLIENEKNETNPFQHLNSKAKMQEKEEEEVVVLKRSSSSCVDIKRHGFWRIGKLFRKNKKNKDCGRSVGGFDEKNEMWMVDHHGGVSRSRSLCSFRGGAIFGSEDGGDSVLSGARSSISAARSSGVNGGLMLESGRRSGYSEAEPRRSDFFYEYENGRKSTGVMEIDGSSYNRRVFSLRESDFKGMDESSFIDLKLDYSSESKMADTLSAFGSIRGGNFMTHDAGGGGGGGDGGSCRFTMNERGIKRERRKSIKGWRWIFKYHSSSRKRDQDLMFKT*