Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037910.01.T01 | XP_013448256.1 | 87.593 | 669 | 19 | 3 | 1 | 605 | 1 | 669 | 0 | 1162 |
| MsG0780037910.01.T02 | XP_013448256.1 | 87.593 | 669 | 19 | 3 | 1 | 605 | 1 | 669 | 0 | 1162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037910.01.T01 | A0A072TZI0 | 87.593 | 669 | 19 | 3 | 1 | 605 | 1 | 669 | 0.0 | 1162 |
| MsG0780037910.01.T02 | A0A072TZI0 | 87.593 | 669 | 19 | 3 | 1 | 605 | 1 | 669 | 0.0 | 1162 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047989.01 | MsG0780037910.01 | 0.829774 | 4.265617e-55 | 4.409297e-52 |
| MsG0080048191.01 | MsG0780037910.01 | 0.812119 | 4.977799e-51 | 3.137724e-48 |
| MsG0080048198.01 | MsG0780037910.01 | 0.810931 | 9.019079e-51 | 5.509084e-48 |
| MsG0080048773.01 | MsG0780037910.01 | 0.867045 | 1.827698e-65 | 6.292353e-62 |
| MsG0080048780.01 | MsG0780037910.01 | 0.815760 | 7.839306e-52 | 5.452978e-49 |
| MsG0080048820.01 | MsG0780037910.01 | 0.824347 | 8.478920e-54 | 7.490268e-51 |
| MsG0080049004.01 | MsG0780037910.01 | 0.802610 | 5.171343e-49 | 2.544249e-46 |
| MsG0180000017.01 | MsG0780037910.01 | 0.817842 | 2.672980e-52 | 1.968346e-49 |
| MsG0180000489.01 | MsG0780037910.01 | 0.838199 | 3.313150e-57 | 4.405253e-54 |
| MsG0180000491.01 | MsG0780037910.01 | 0.823731 | 1.183005e-53 | 1.027236e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037910.01.T01 | MTR_7g038690 | 87.593 | 669 | 19 | 3 | 1 | 605 | 1 | 669 | 0.0 | 1162 |
| MsG0780037910.01.T01 | MTR_7g030070 | 84.903 | 669 | 21 | 4 | 1 | 605 | 1 | 653 | 0.0 | 1116 |
| MsG0780037910.01.T01 | MTR_7g038690 | 76.024 | 659 | 59 | 5 | 1 | 605 | 1 | 614 | 0.0 | 959 |
| MsG0780037910.01.T01 | MTR_6g060230 | 54.042 | 668 | 240 | 6 | 3 | 603 | 12 | 679 | 0.0 | 681 |
| MsG0780037910.01.T01 | MTR_5g011840 | 47.710 | 677 | 270 | 11 | 4 | 602 | 6 | 676 | 0.0 | 564 |
| MsG0780037910.01.T01 | MTR_5g011840 | 45.277 | 667 | 263 | 13 | 4 | 602 | 6 | 638 | 2.13e-172 | 504 |
| MsG0780037910.01.T01 | MTR_5g011840 | 46.463 | 523 | 210 | 9 | 144 | 602 | 1 | 517 | 6.48e-139 | 414 |
| MsG0780037910.01.T01 | MTR_5g033820 | 26.772 | 635 | 337 | 17 | 18 | 605 | 31 | 584 | 5.27e-57 | 203 |
| MsG0780037910.01.T01 | MTR_7g073710 | 28.393 | 641 | 361 | 20 | 4 | 598 | 12 | 600 | 4.57e-55 | 197 |
| MsG0780037910.01.T01 | MTR_3g093930 | 30.528 | 606 | 312 | 21 | 67 | 601 | 407 | 974 | 5.96e-55 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037910.01.T01 | AT2G45340 | 54.932 | 659 | 219 | 12 | 23 | 603 | 28 | 686 | 0.0 | 664 |
| MsG0780037910.01.T01 | AT5G51560 | 51.376 | 654 | 238 | 11 | 21 | 603 | 31 | 675 | 0.0 | 601 |
| MsG0780037910.01.T01 | AT4G22730 | 46.824 | 677 | 277 | 12 | 2 | 598 | 6 | 679 | 0.0 | 570 |
| MsG0780037910.01.T01 | AT4G22730 | 44.402 | 527 | 211 | 11 | 2 | 449 | 6 | 529 | 6.26e-133 | 399 |
| MsG0780037910.01.T01 | AT1G31420 | 28.639 | 632 | 316 | 21 | 15 | 598 | 27 | 571 | 2.55e-57 | 203 |
| MsG0780037910.01.T01 | AT1G31420 | 28.639 | 632 | 316 | 21 | 15 | 598 | 27 | 571 | 2.55e-57 | 203 |
| MsG0780037910.01.T01 | AT3G56370 | 31.419 | 592 | 319 | 18 | 67 | 598 | 381 | 945 | 2.01e-56 | 206 |
| MsG0780037910.01.T01 | AT3G56370 | 36.667 | 150 | 85 | 1 | 74 | 213 | 203 | 352 | 6.72e-18 | 88.6 |
| MsG0780037910.01.T01 | AT3G56370 | 33.005 | 203 | 104 | 3 | 40 | 215 | 246 | 443 | 1.92e-17 | 87.0 |
| MsG0780037910.01.T01 | AT3G56370 | 34.025 | 241 | 117 | 8 | 18 | 219 | 25 | 262 | 9.23e-17 | 84.7 |
Find 40 sgRNAs with CRISPR-Local
Find 284 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCCTTGCTTGCAGATTC+AGG | 0.318132 | 7:+36266469 | MsG0780037910.01.T01:CDS |
| AACTACCACTTTCTACACAT+TGG | 0.543294 | 7:-36266675 | None:intergenic |
| AAGAGTGACGTGTATGCTTT+TGG | 0.274261 | 7:+36266598 | MsG0780037910.01.T01:CDS |
| ACCATTTATGACTCAGTTCA+AGG | 0.378462 | 7:-36266825 | None:intergenic |
| AGAAAACAACATCATCTGCA+AGG | 0.652573 | 7:-36266504 | None:intergenic |
| AGAATCAGAGGCAGAGAAAT+TGG | 0.330584 | 7:+36266738 | MsG0780037910.01.T01:CDS |
| AGCAGCACTAGCTTTGAGGG+TGG | 0.642811 | 7:-36266527 | None:intergenic |
| AGGATTACATTCTCCATAGT+TGG | 0.403651 | 7:-36266805 | None:intergenic |
| AGTCGCCAATGTGTAGAAAG+TGG | 0.560757 | 7:+36266670 | MsG0780037910.01.T01:CDS |
| ATCATCTCCCATTCTAAAAT+TGG | 0.191164 | 7:-36267005 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAAATGGCCCTTTCTA+AGG | + | Chr7:36265989-36266008 | MsG0780037910.01.T01:intron | 30.0% | |
| AAATAATCATGCCTAAATGC+AGG | - | Chr7:36263759-36263778 | None:intergenic | 30.0% | |
| AACCAAAGTCTAAAAACTGT+GGG | + | Chr7:36264988-36265007 | MsG0780037910.01.T01:CDS | 30.0% | |
| AAGTAGAAAAACTCATGTAG+TGG | + | Chr7:36263843-36263862 | MsG0780037910.01.T01:intron | 30.0% | |
| ACTTACAATATCGTCTTGTA+AGG | + | Chr7:36266132-36266151 | MsG0780037910.01.T01:intron | 30.0% | |
| ATAAGAAACAATTCACTCTC+TGG | + | Chr7:36264520-36264539 | MsG0780037910.01.T01:CDS | 30.0% | |
| ATGACCTATACAATCTTGAT+CGG | - | Chr7:36264066-36264085 | None:intergenic | 30.0% | |
| ATTATTGACGAGAACCTTAA+CGG | + | Chr7:36266706-36266725 | MsG0780037910.01.T01:CDS | 30.0% | |
| CACTTTAGTTAGGTAATGAA+AGG | + | Chr7:36263389-36263408 | MsG0780037910.01.T01:intron | 30.0% | |
| CCGTTGAAATTTCAGATAAT+CGG | + | Chr7:36265149-36265168 | MsG0780037910.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 36262534 | 36267199 | 36262534 | ID=MsG0780037910.01;Name=MsG0780037910.01 |
| Chr7 | mRNA | 36262534 | 36267199 | 36262534 | ID=MsG0780037910.01.T01;Parent=MsG0780037910.01;Name=MsG0780037910.01.T01;_AED=0.44;_eAED=0.44;_QI=44|0.5|0.42|1|0.83|0.71|7|250|605 |
| Chr7 | exon | 36267004 | 36267199 | 36267004 | ID=MsG0780037910.01.T01:exon:10367;Parent=MsG0780037910.01.T01 |
| Chr7 | exon | 36266377 | 36266903 | 36266377 | ID=MsG0780037910.01.T01:exon:10366;Parent=MsG0780037910.01.T01 |
| Chr7 | five_prime_UTR | 36262534 | 36262577 | 36262534 | ID=MsG0780037910.01.T01:five_prime_utr;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36262578 | 36262989 | 36262578 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36264326 | 36264556 | 36264326 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36264820 | 36265064 | 36264820 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36265136 | 36265478 | 36265136 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36265568 | 36265681 | 36265568 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | CDS | 36266377 | 36266849 | 36266377 | ID=MsG0780037910.01.T01:cds;Parent=MsG0780037910.01.T01 |
| Chr7 | three_prime_UTR | 36266850 | 36266903 | 36266850 | ID=MsG0780037910.01.T01:three_prime_utr;Parent=MsG0780037910.01.T01 |
| Chr7 | three_prime_UTR | 36267004 | 36267199 | 36267004 | ID=MsG0780037910.01.T01:three_prime_utr;Parent=MsG0780037910.01.T01 |
| Chr7 | mRNA | 36262534 | 36267140 | 36262534 | ID=MsG0780037910.01.T02;Parent=MsG0780037910.01;Name=MsG0780037910.01.T02;_AED=0.44;_eAED=0.44;_QI=44|0.4|0.33|1|1|1|6|291|605 |
| Chr7 | exon | 36266377 | 36267140 | 36266377 | ID=MsG0780037910.01.T02:exon:10368;Parent=MsG0780037910.01.T02 |
| Chr7 | five_prime_UTR | 36262534 | 36262577 | 36262534 | ID=MsG0780037910.01.T02:five_prime_utr;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36262578 | 36262989 | 36262578 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36264326 | 36264556 | 36264326 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36264820 | 36265064 | 36264820 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36265136 | 36265478 | 36265136 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36265568 | 36265681 | 36265568 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | CDS | 36266377 | 36266849 | 36266377 | ID=MsG0780037910.01.T02:cds;Parent=MsG0780037910.01.T02 |
| Chr7 | three_prime_UTR | 36266850 | 36267140 | 36266850 | ID=MsG0780037910.01.T02:three_prime_utr;Parent=MsG0780037910.01.T02 |
| Gene Sequence |
>MsG0780037910.01.T02 ATGTCTTCTCTCATATTTCTACTGTTCATATTCGTATCTCACTCTCTTTCCCTTAACACTGAACTTGACACTCTCATCCTCATTAAAGACTCTTTAGACCCAGAAAACCATGTGTTACTCTCATGGAACAATCATTCTGACCCTTGTAGTGGAACTTTTGAAGGTGTAGCTTGTAATGAACAAGGTCTTGTTACTAATATATCTCTTCAAGGAAAAGGTTTATCTGGTGAAATACCTTCAGTGATTGGTAAACTTAAGAGTTTAACTGGTCTTTACCTTCATTTTAATGCTCTTAATGGTATTTTGCCTAAAGAAATTGCTGGTTTAACTCAGCTTAGTGACTTGTATCTTAATGTTAATAATCTCTCTGGTTTTATCCCTCATGAGATTGGTAACATGTCTAATCTTCAAGGAAGTATACCTACCGAGCTTGGAAAATTAAAGAGGCTAAGCGTTCTTGCGCTCCAATATAATCACTTAAGTGGCGCAATTCCTGCAAGCTTGGGTGAGTTGGAGACACTTGAAAGATTGGATCTCAGCTTCAATACCCTTTTGGGTCCAATTCCAGTAACATTGGCTAATGCACCTAAACTAGAGACTCTTGATATAAGAAACAATTCACTCTCTGGCAGCGTCCCTACAGATCTGAAAAGACTAAAAGAAGGGTTCCAATATTTTAACAATCATGGTTTATGTGGCACTGGATTTGCACACTTAGATTCTTGTCAAATAGTTAGCAATTCAGACCCAGTTAGACCTGAGCCATATGACCCAAGTAATATTTCCACAATTGAATTTCCGACAACACCTGAACCAAAGTCTAAAAACTGTGGGAATTCTGGTTGTAGAAGGCGGTCAGATTCTTCCACGATTGGTTTGGTTTTTGCGAAAATTGGAAACACCGTTGAAATTTCAGATAATCGGCCTAGTACCGACAAGATCAAGGAAGTATACAGGAAAAAGGCCTCTCCGCTAATAAATTTGGAGTACTCAAGTGGATGGGATCCATTATCAAAAGATCTTGGTGGTTATTCGCAAGAATTTCTCCAGAGCTTTATGTTTAATCTCGAAGAAGTAGACCGCGCGACTCAGTGCTTCTCAGAGATGAATTTATTGGCAAAGAATAATATTTCAGCTAACTATAGAGGAACCTTGCGAGATGGATCTATTGTGGTTATAAAGTGCATTGCCAAAACAACCTGCAAATCTGACGAAACTGAATTCTTGAATGTTTATGATTTTGTCTCAAATGGGAGTTTGTCAAAGTATCTTGATGTTCAGAGAGAAAGTGCTGAGGTGCTTGAATGGTCTACTAGAGTGTCCATAATTCATGGCATTGCCAAAGGTATTTCTTATCTGCATGGAAAGAAAGGAAGGAAGCATTCTCTAGTTCATCAGAGTATATCGGCTGAAAAAGTACTTCTCGATTCTCAATACAAATCCTTGCTTGCAGATTCAGGATTGCACAAACTCCTTGCAGATGATGTTGTTTTCTCCACCCTCAAAGCTAGTGCTGCTATGGGGTACCTGGCACCAGAATACACAACAACCGGTCGTTTCACCGAAAAGAGTGACGTGTATGCTTTTGGGGTGATTGTTTTCCAGATTCTCACTGGAAAACACGATATCACTCAGTTAAGTCGCCAATGTGTAGAAAGTGGTAGTTTGAAAGATATTATTGACGAGAACCTTAACGGGAAGTTCTTAGAATCAGAGGCAGAGAAATTGGCTAGACTCGCTTTGGTTTGCACCGATGAATCTCCTAATCTCCGGCCAACTATGGAGAATGTAATCCTTGAACTGAGTCATAAATGGTAA >MsG0780037910.01.T01 ATGTCTTCTCTCATATTTCTACTGTTCATATTCGTATCTCACTCTCTTTCCCTTAACACTGAACTTGACACTCTCATCCTCATTAAAGACTCTTTAGACCCAGAAAACCATGTGTTACTCTCATGGAACAATCATTCTGACCCTTGTAGTGGAACTTTTGAAGGTGTAGCTTGTAATGAACAAGGTCTTGTTACTAATATATCTCTTCAAGGAAAAGGTTTATCTGGTGAAATACCTTCAGTGATTGGTAAACTTAAGAGTTTAACTGGTCTTTACCTTCATTTTAATGCTCTTAATGGTATTTTGCCTAAAGAAATTGCTGGTTTAACTCAGCTTAGTGACTTGTATCTTAATGTTAATAATCTCTCTGGTTTTATCCCTCATGAGATTGGTAACATGTCTAATCTTCAAGGAAGTATACCTACCGAGCTTGGAAAATTAAAGAGGCTAAGCGTTCTTGCGCTCCAATATAATCACTTAAGTGGCGCAATTCCTGCAAGCTTGGGTGAGTTGGAGACACTTGAAAGATTGGATCTCAGCTTCAATACCCTTTTGGGTCCAATTCCAGTAACATTGGCTAATGCACCTAAACTAGAGACTCTTGATATAAGAAACAATTCACTCTCTGGCAGCGTCCCTACAGATCTGAAAAGACTAAAAGAAGGGTTCCAATATTTTAACAATCATGGTTTATGTGGCACTGGATTTGCACACTTAGATTCTTGTCAAATAGTTAGCAATTCAGACCCAGTTAGACCTGAGCCATATGACCCAAGTAATATTTCCACAATTGAATTTCCGACAACACCTGAACCAAAGTCTAAAAACTGTGGGAATTCTGGTTGTAGAAGGCGGTCAGATTCTTCCACGATTGGTTTGGTTTTTGCGAAAATTGGAAACACCGTTGAAATTTCAGATAATCGGCCTAGTACCGACAAGATCAAGGAAGTATACAGGAAAAAGGCCTCTCCGCTAATAAATTTGGAGTACTCAAGTGGATGGGATCCATTATCAAAAGATCTTGGTGGTTATTCGCAAGAATTTCTCCAGAGCTTTATGTTTAATCTCGAAGAAGTAGACCGCGCGACTCAGTGCTTCTCAGAGATGAATTTATTGGCAAAGAATAATATTTCAGCTAACTATAGAGGAACCTTGCGAGATGGATCTATTGTGGTTATAAAGTGCATTGCCAAAACAACCTGCAAATCTGACGAAACTGAATTCTTGAATGTTTATGATTTTGTCTCAAATGGGAGTTTGTCAAAGTATCTTGATGTTCAGAGAGAAAGTGCTGAGGTGCTTGAATGGTCTACTAGAGTGTCCATAATTCATGGCATTGCCAAAGGTATTTCTTATCTGCATGGAAAGAAAGGAAGGAAGCATTCTCTAGTTCATCAGAGTATATCGGCTGAAAAAGTACTTCTCGATTCTCAATACAAATCCTTGCTTGCAGATTCAGGATTGCACAAACTCCTTGCAGATGATGTTGTTTTCTCCACCCTCAAAGCTAGTGCTGCTATGGGGTACCTGGCACCAGAATACACAACAACCGGTCGTTTCACCGAAAAGAGTGACGTGTATGCTTTTGGGGTGATTGTTTTCCAGATTCTCACTGGAAAACACGATATCACTCAGTTAAGTCGCCAATGTGTAGAAAGTGGTAGTTTGAAAGATATTATTGACGAGAACCTTAACGGGAAGTTCTTAGAATCAGAGGCAGAGAAATTGGCTAGACTCGCTTTGGTTTGCACCGATGAATCTCCTAATCTCCGGCCAACTATGGAGAATGTAATCCTTGAACTGAGTCATAAATGGTAA |
| Protein sequence |
>MsG0780037910.01.T02 MSSLIFLLFIFVSHSLSLNTELDTLILIKDSLDPENHVLLSWNNHSDPCSGTFEGVACNEQGLVTNISLQGKGLSGEIPSVIGKLKSLTGLYLHFNALNGILPKEIAGLTQLSDLYLNVNNLSGFIPHEIGNMSNLQGSIPTELGKLKRLSVLALQYNHLSGAIPASLGELETLERLDLSFNTLLGPIPVTLANAPKLETLDIRNNSLSGSVPTDLKRLKEGFQYFNNHGLCGTGFAHLDSCQIVSNSDPVRPEPYDPSNISTIEFPTTPEPKSKNCGNSGCRRRSDSSTIGLVFAKIGNTVEISDNRPSTDKIKEVYRKKASPLINLEYSSGWDPLSKDLGGYSQEFLQSFMFNLEEVDRATQCFSEMNLLAKNNISANYRGTLRDGSIVVIKCIAKTTCKSDETEFLNVYDFVSNGSLSKYLDVQRESAEVLEWSTRVSIIHGIAKGISYLHGKKGRKHSLVHQSISAEKVLLDSQYKSLLADSGLHKLLADDVVFSTLKASAAMGYLAPEYTTTGRFTEKSDVYAFGVIVFQILTGKHDITQLSRQCVESGSLKDIIDENLNGKFLESEAEKLARLALVCTDESPNLRPTMENVILELSHKW* >MsG0780037910.01.T01 MSSLIFLLFIFVSHSLSLNTELDTLILIKDSLDPENHVLLSWNNHSDPCSGTFEGVACNEQGLVTNISLQGKGLSGEIPSVIGKLKSLTGLYLHFNALNGILPKEIAGLTQLSDLYLNVNNLSGFIPHEIGNMSNLQGSIPTELGKLKRLSVLALQYNHLSGAIPASLGELETLERLDLSFNTLLGPIPVTLANAPKLETLDIRNNSLSGSVPTDLKRLKEGFQYFNNHGLCGTGFAHLDSCQIVSNSDPVRPEPYDPSNISTIEFPTTPEPKSKNCGNSGCRRRSDSSTIGLVFAKIGNTVEISDNRPSTDKIKEVYRKKASPLINLEYSSGWDPLSKDLGGYSQEFLQSFMFNLEEVDRATQCFSEMNLLAKNNISANYRGTLRDGSIVVIKCIAKTTCKSDETEFLNVYDFVSNGSLSKYLDVQRESAEVLEWSTRVSIIHGIAKGISYLHGKKGRKHSLVHQSISAEKVLLDSQYKSLLADSGLHKLLADDVVFSTLKASAAMGYLAPEYTTTGRFTEKSDVYAFGVIVFQILTGKHDITQLSRQCVESGSLKDIIDENLNGKFLESEAEKLARLALVCTDESPNLRPTMENVILELSHKW* |
