AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030767.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030767.01.T01 MTR_6g012280 92.982 114 3 3 1 112 218 328 6.48e-69 210
MsG0680030767.01.T01 MTR_7g010300 49.624 133 38 3 4 117 219 341 1.58e-33 119
MsG0680030767.01.T01 MTR_4g134580 60.526 76 30 0 5 80 284 359 8.99e-28 104
MsG0680030767.01.T01 MTR_8g467340 66.176 68 23 0 5 72 271 338 4.93e-27 102
MsG0680030767.01.T01 MTR_5g032600 69.841 63 19 0 5 67 209 271 1.42e-26 99.8
MsG0680030767.01.T01 MTR_5g065440 66.667 63 21 0 5 67 205 267 1.99e-25 96.7
MsG0680030767.01.T01 MTR_3g100650 64.062 64 23 0 4 67 210 273 3.45e-25 96.3
MsG0680030767.01.T01 MTR_5g020510 57.812 64 27 0 4 67 232 295 7.60e-24 93.2
MsG0680030767.01.T01 MTR_1g077020 58.824 68 27 1 5 71 124 191 6.91e-23 88.2
MsG0680030767.01.T01 MTR_2g076780 59.375 64 26 0 4 67 173 236 9.00e-22 86.7
MsG0680030767.01.T01 MTR_8g069380 59.375 64 26 0 4 67 51 114 1.04e-21 83.2
MsG0680030767.01.T01 MTR_6g073013 60.000 60 23 1 5 63 101 160 2.53e-21 84.0
MsG0680030767.01.T01 MTR_8g051480 59.375 64 25 1 4 67 66 128 3.95e-20 79.7
MsG0680030767.01.T01 MTR_8g069080 58.065 62 26 0 6 67 206 267 2.65e-19 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030767.01.T01 AT5G39760 76.562 64 15 0 4 67 199 262 2.21e-31 113
MsG0680030767.01.T01 AT3G28920 73.770 61 16 0 4 64 191 251 8.93e-30 108
MsG0680030767.01.T01 AT4G24660 63.636 66 24 0 2 67 154 219 1.00e-25 96.3
MsG0680030767.01.T01 AT2G02540 63.492 63 23 0 5 67 222 284 1.94e-25 97.4
MsG0680030767.01.T01 AT2G02540 63.492 63 23 0 5 67 222 284 1.94e-25 97.4
MsG0680030767.01.T01 AT5G15210 65.574 61 21 0 1 61 175 235 2.25e-25 96.7
MsG0680030767.01.T01 AT5G65410 55.556 72 32 0 2 73 188 259 2.30e-25 96.7
MsG0680030767.01.T01 AT1G75240 64.516 62 22 0 1 62 236 297 1.15e-24 95.5
MsG0680030767.01.T01 AT4G24660 63.492 63 23 0 2 64 154 216 5.98e-24 92.0
MsG0680030767.01.T01 AT1G14440 63.492 63 23 0 5 67 218 280 1.53e-23 92.4
MsG0680030767.01.T01 AT1G14440 63.492 63 23 0 5 67 218 280 1.53e-23 92.4
MsG0680030767.01.T01 AT1G69600 65.079 63 22 0 2 64 153 215 2.41e-23 90.9
MsG0680030767.01.T01 AT2G18350 59.375 64 26 0 4 67 197 260 1.58e-22 89.0
MsG0680030767.01.T01 AT3G50890 57.895 57 24 0 10 66 186 242 1.87e-19 80.5
MsG0680030767.01.T01 AT5G60480 59.649 57 23 0 5 61 147 203 1.03e-17 75.5
MsG0680030767.01.T01 AT5G42780 45.763 59 32 0 6 64 180 238 3.13e-14 66.6

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCATCAGATCATGTTAA+TGG 0.269168 6:-8809386 MsG0680030767.01.T01:CDS
GCTGCTTGTGCTACTACTAA+TGG 0.296309 6:-8809278 MsG0680030767.01.T01:CDS
ATAGAAGTGTTCTCAAAGTT+TGG 0.303184 6:-8809445 MsG0680030767.01.T01:CDS
GCAGAAGAGAGATGAAGAAA+TGG 0.377517 6:-8809498 MsG0680030767.01.T01:CDS
GCCACCGTGGACGGTGGCTC+CGG 0.383852 6:-8809344 MsG0680030767.01.T01:CDS
GAGAATGAGAATGAGAATAA+TGG 0.393072 6:-8809311 MsG0680030767.01.T01:CDS
CATAATAATAAGAACACTTT+GGG 0.393395 6:-8809419 MsG0680030767.01.T01:CDS
CTGCTTGTGCTACTACTAAT+GGG 0.418784 6:-8809277 MsG0680030767.01.T01:CDS
ACCGTCCACGGTGGCGTTCA+CGG 0.433426 6:+8809352 None:intergenic
CATGAATTTGCTGATAAAGT+TGG 0.436563 6:-8809530 MsG0680030767.01.T01:CDS
GCATAATAATAAGAACACTT+TGG 0.453675 6:-8809420 MsG0680030767.01.T01:CDS
AGAATGAGAATGAGAATAAT+GGG 0.469330 6:-8809310 MsG0680030767.01.T01:CDS
GCCGTGAACGCCACCGTGGA+CGG 0.475223 6:-8809353 MsG0680030767.01.T01:CDS
GTGGACGGTGGCTCCGGTGG+TGG 0.498982 6:-8809338 MsG0680030767.01.T01:CDS
CTCATTCTCACAACCACCAC+CGG 0.531538 6:+8809325 None:intergenic
ATGAATTTGCTGATAAAGTT+GGG 0.532314 6:-8809529 MsG0680030767.01.T01:CDS
AGAGATGAAGAAATGGTGAA+TGG 0.553318 6:-8809491 MsG0680030767.01.T01:CDS
ACCACCGGAGCCACCGTCCA+CGG 0.559923 6:+8809340 None:intergenic
AAAATTCACACAAGAACAGA+AGG 0.564253 6:-8809561 MsG0680030767.01.T01:CDS
ACCGTGGACGGTGGCTCCGG+TGG 0.586098 6:-8809341 MsG0680030767.01.T01:CDS
GTGAACGCCACCGTGGACGG+TGG 0.613575 6:-8809350 MsG0680030767.01.T01:CDS
CGCCGCCGTGAACGCCACCG+TGG 0.668018 6:-8809357 MsG0680030767.01.T01:CDS
AATTTGCTGATAAAGTTGGG+TGG 0.672155 6:-8809526 MsG0680030767.01.T01:CDS
ACCGGAGCCACCGTCCACGG+TGG 0.716730 6:+8809343 None:intergenic
GTCCACGGTGGCGTTCACGG+CGG 0.745811 6:+8809355 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAATAATAAGAACACTTT+GGG - Chr6:8809430-8809449 MsG0680030767.01.T01:CDS 20.0%
! AGAATGAGAATGAGAATAAT+GGG - Chr6:8809539-8809558 MsG0680030767.01.T01:CDS 25.0%
! GCATAATAATAAGAACACTT+TGG - Chr6:8809429-8809448 MsG0680030767.01.T01:CDS 25.0%
!!! ATGAATTTGCTGATAAAGTT+GGG - Chr6:8809320-8809339 MsG0680030767.01.T01:CDS 25.0%
AAAATTCACACAAGAACAGA+AGG - Chr6:8809288-8809307 MsG0680030767.01.T01:CDS 30.0%
GAGAATGAGAATGAGAATAA+TGG - Chr6:8809538-8809557 MsG0680030767.01.T01:CDS 30.0%
TCATCATCAGATCATGTTAA+TGG - Chr6:8809463-8809482 MsG0680030767.01.T01:CDS 30.0%
! AATGGTTTTTGCAATGAAGT+TGG - Chr6:8809376-8809395 MsG0680030767.01.T01:CDS 30.0%
!! ATAGAAGTGTTCTCAAAGTT+TGG - Chr6:8809404-8809423 MsG0680030767.01.T01:CDS 30.0%
!! CATGAATTTGCTGATAAAGT+TGG - Chr6:8809319-8809338 MsG0680030767.01.T01:CDS 30.0%
AGAGATGAAGAAATGGTGAA+TGG - Chr6:8809358-8809377 MsG0680030767.01.T01:CDS 35.0%
!! AATTTGCTGATAAAGTTGGG+TGG - Chr6:8809323-8809342 MsG0680030767.01.T01:CDS 35.0%
GCAGAAGAGAGATGAAGAAA+TGG - Chr6:8809351-8809370 MsG0680030767.01.T01:CDS 40.0%
! CTGCTTGTGCTACTACTAAT+GGG - Chr6:8809572-8809591 MsG0680030767.01.T01:CDS 40.0%
! GCTGCTTGTGCTACTACTAA+TGG - Chr6:8809571-8809590 MsG0680030767.01.T01:CDS 45.0%
CTCATTCTCACAACCACCAC+CGG + Chr6:8809527-8809546 None:intergenic 50.0%
ACCGTCCACGGTGGCGTTCA+CGG + Chr6:8809500-8809519 None:intergenic 65.0%
ACCACCGGAGCCACCGTCCA+CGG + Chr6:8809512-8809531 None:intergenic 70.0%
GCCGTGAACGCCACCGTGGA+CGG - Chr6:8809496-8809515 MsG0680030767.01.T01:CDS 70.0%
GTCCACGGTGGCGTTCACGG+CGG + Chr6:8809497-8809516 None:intergenic 70.0%
GTGAACGCCACCGTGGACGG+TGG - Chr6:8809499-8809518 MsG0680030767.01.T01:CDS 70.0%
ACCGGAGCCACCGTCCACGG+TGG + Chr6:8809509-8809528 None:intergenic 75.0%
ACCGTGGACGGTGGCTCCGG+TGG - Chr6:8809508-8809527 MsG0680030767.01.T01:CDS 75.0%
GCCACCGTGGACGGTGGCTC+CGG - Chr6:8809505-8809524 MsG0680030767.01.T01:CDS 75.0%
GTGGACGGTGGCTCCGGTGG+TGG - Chr6:8809511-8809530 MsG0680030767.01.T01:CDS 75.0%
! CGCCGCCGTGAACGCCACCG+TGG - Chr6:8809492-8809511 MsG0680030767.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr6 gene 8809259 8809612 8809259 ID=MsG0680030767.01;Name=MsG0680030767.01
Chr6 mRNA 8809259 8809612 8809259 ID=MsG0680030767.01.T01;Parent=MsG0680030767.01;Name=MsG0680030767.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|117
Chr6 exon 8809259 8809612 8809259 ID=MsG0680030767.01.T01:exon:7271;Parent=MsG0680030767.01.T01
Chr6 CDS 8809259 8809612 8809259 ID=MsG0680030767.01.T01:cds;Parent=MsG0680030767.01.T01
Gene Sequence

>MsG0680030767.01.T01

ATGGGAACAAGTAGAAAAAGATTCAGAACAAAATTCACACAAGAACAGAAGGAAAAGATGCATGAATTTGCTGATAAAGTTGGGTGGAAGATGCAGAAGAGAGATGAAGAAATGGTGAATGGTTTTTGCAATGAAGTTGGTGTTGATAGAAGTGTTCTCAAAGTTTGGATGCATAATAATAAGAACACTTTGGGAAGAAAATTATCATCATCAGATCATGTTAATGGTGACAGCGCCGCCGTGAACGCCACCGTGGACGGTGGCTCCGGTGGTGGTTGTGAGAATGAGAATGAGAATAATGGGAGTAATGTTGCTGCTTGTGCTACTACTAATGGGTCTTCTTCTTCTTCTTGA

Protein sequence

>MsG0680030767.01.T01

MGTSRKRFRTKFTQEQKEKMHEFADKVGWKMQKRDEEMVNGFCNEVGVDRSVLKVWMHNNKNTLGRKLSSSDHVNGDSAAVNATVDGGSGGGCENENENNGSNVAACATTNGSSSSS*