AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0296740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296740 MtrunA17_Chr6g0453811 96.923 65 2 0 1 65 1 65 1.44e-40 126
Msa0296740 MtrunA17_Chr4g0041861 87.692 65 8 0 1 65 1 65 5.31e-36 115
Msa0296740 MtrunA17_Chr6g0453911 80.000 65 12 1 1 65 1 64 7.50e-30 99.8
Msa0296740 MtrunA17_Chr8g0381481 85.714 49 7 0 1 49 1 49 3.27e-25 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296740 AT5G03850.1 87.692 65 7 1 1 65 1 64 3.84e-34 110
Msa0296740 AT3G10090.1 87.692 65 7 1 1 65 1 64 3.84e-34 110
Msa0296740 AT5G64140.1 86.154 65 8 1 1 65 1 64 1.89e-33 109

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATTGTTGTGAAAGTTAT+GGG 0.389099 2_3:-50197904 Msa0296740:CDS
AGAATGTATCCCTCCCTAAC+TGG 0.428755 2_3:+50197793 None:intergenic
TGCTATTGTTGTGAAAGTTA+TGG 0.460517 2_3:-50197905 Msa0296740:CDS
GAATGTGAAGGGACCAGTTA+GGG 0.464736 2_3:-50197806 Msa0296740:CDS
AACCTGAGTGACTTGTCCTC+TGG 0.467654 2_3:+50197870 None:intergenic
GGAATGTGAAGGGACCAGTT+AGG 0.473428 2_3:-50197807 Msa0296740:CDS
TTGCTTCTCTCTCAGACTCA+AGG 0.498678 2_3:+50197764 None:intergenic
GTGAAAGTTATGGGTCGTAC+TGG 0.530087 2_3:-50197895 Msa0296740:CDS
TGTGAAGGGACCAGTTAGGG+AGG 0.582336 2_3:-50197803 Msa0296740:CDS
ATCAGAATCGTCACATCATG+AGG 0.585221 2_3:-50197828 Msa0296740:CDS
CTTCTCTCTCAGACTCAAGG+AGG 0.589933 2_3:+50197767 None:intergenic
TCACATCATGAGGAATGTGA+AGG 0.626309 2_3:-50197818 Msa0296740:CDS
CACATCATGAGGAATGTGAA+GGG 0.632733 2_3:-50197817 Msa0296740:CDS
ATCCAGAGGACAAGTCACTC+AGG 0.638739 2_3:-50197872 Msa0296740:CDS
TTCTCTCTCAGACTCAAGGA+GGG 0.640280 2_3:+50197768 None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG 0.657315 2_3:-50197802 Msa0296740:CDS
ATGGGTCGTACTGGATCCAG+AGG 0.663896 2_3:-50197886 Msa0296740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGCTATTGTTGTGAAAGTTA+TGG - chr2_3:50197770-50197789 Msa0296740:CDS 30.0%
GCTATTGTTGTGAAAGTTAT+GGG - chr2_3:50197771-50197790 Msa0296740:CDS 30.0%
! TCAGGTTAGAGTGAAGTTTT+TGG - chr2_3:50197821-50197840 Msa0296740:CDS 35.0%
ATCAGAATCGTCACATCATG+AGG - chr2_3:50197847-50197866 Msa0296740:CDS 40.0%
TCACATCATGAGGAATGTGA+AGG - chr2_3:50197857-50197876 Msa0296740:CDS 40.0%
CACATCATGAGGAATGTGAA+GGG - chr2_3:50197858-50197877 Msa0296740:CDS 40.0%
GTGAAAGTTATGGGTCGTAC+TGG - chr2_3:50197780-50197799 Msa0296740:CDS 45.0%
GAATGTGAAGGGACCAGTTA+GGG - chr2_3:50197869-50197888 Msa0296740:CDS 45.0%
AGAATGTATCCCTCCCTAAC+TGG + chr2_3:50197885-50197904 None:intergenic 45.0%
TTCTCTCTCAGACTCAAGGA+GGG + chr2_3:50197910-50197929 None:intergenic 45.0%
TTGCTTCTCTCTCAGACTCA+AGG + chr2_3:50197914-50197933 None:intergenic 45.0%
ATCCAGAGGACAAGTCACTC+AGG - chr2_3:50197803-50197822 Msa0296740:CDS 50.0%
! AACCTGAGTGACTTGTCCTC+TGG + chr2_3:50197808-50197827 None:intergenic 50.0%
GGAATGTGAAGGGACCAGTT+AGG - chr2_3:50197868-50197887 Msa0296740:CDS 50.0%
CTTCTCTCTCAGACTCAAGG+AGG + chr2_3:50197911-50197930 None:intergenic 50.0%
ATGGGTCGTACTGGATCCAG+AGG - chr2_3:50197789-50197808 Msa0296740:CDS 55.0%
TGTGAAGGGACCAGTTAGGG+AGG - chr2_3:50197872-50197891 Msa0296740:CDS 55.0%
GTGAAGGGACCAGTTAGGGA+GGG - chr2_3:50197873-50197892 Msa0296740:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 50197750 50197947 50197750 ID=Msa0296740;Name=Msa0296740
chr2_3 mRNA 50197750 50197947 50197750 ID=Msa0296740-mRNA-1;Parent=Msa0296740;Name=Msa0296740-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|65
chr2_3 exon 50197750 50197947 50197750 ID=Msa0296740-mRNA-1:exon:15790;Parent=Msa0296740-mRNA-1
chr2_3 CDS 50197750 50197947 50197750 ID=Msa0296740-mRNA-1:cds;Parent=Msa0296740-mRNA-1
Gene Sequence

>Msa0296740

ATGGAATCACAGATTAAGCATGCTATTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGAGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGACGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGATACATTCTCACCCTCCTTGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG

Protein sequence

>Msa0296740

MESQIKHAIVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGYILTLLESEREARRLR*