AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1231240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231240 MtrunA17_Chr8g0340131 91.983 237 16 2 1 237 1 234 4.73e-123 348
Msa1231240 MtrunA17_Chr7g0236051 51.327 113 44 2 125 237 238 339 6.09e-29 111
Msa1231240 MtrunA17_Chr4g0075411 83.673 49 8 0 189 237 169 217 7.78e-27 103
Msa1231240 MtrunA17_Chr2g0289321 67.742 62 20 0 176 237 152 213 3.42e-26 101
Msa1231240 MtrunA17_Chr3g0110201 49.107 112 49 2 126 237 249 352 4.40e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231240 AT3G25030.3 35.246 244 108 7 3 237 8 210 1.50e-36 128
Msa1231240 AT3G25030.1 35.039 254 135 7 3 237 8 250 6.02e-35 125
Msa1231240 AT3G25030.4 35.039 254 135 7 3 237 8 250 6.02e-35 125
Msa1231240 AT3G25030.2 35.039 254 135 7 3 237 8 250 6.02e-35 125
Msa1231240 AT4G13100.5 31.206 282 114 6 3 237 8 256 3.73e-32 118
Msa1231240 AT4G13100.3 31.206 282 114 6 3 237 8 256 3.73e-32 118
Msa1231240 AT4G13100.8 31.522 276 124 8 3 237 8 259 4.38e-31 115
Msa1231240 AT4G13100.7 31.522 276 124 8 3 237 8 259 4.38e-31 115
Msa1231240 AT4G13100.9 31.522 276 124 8 3 237 8 259 4.38e-31 115
Msa1231240 AT4G13100.1 31.522 276 124 8 3 237 8 259 4.38e-31 115
Msa1231240 AT4G13100.4 31.522 276 124 8 3 237 5 256 5.22e-31 115
Msa1231240 AT4G13100.2 49.593 123 43 2 119 237 158 265 1.94e-30 114
Msa1231240 AT4G22250.1 58.025 81 34 0 157 237 134 214 1.20e-27 105
Msa1231240 AT4G03965.1 45.000 120 42 2 121 237 111 209 2.73e-26 101
Msa1231240 AT1G62370.1 71.429 49 14 0 189 237 156 204 1.68e-23 94.4
Msa1231240 AT3G07120.1 45.370 108 54 1 130 237 258 360 6.33e-23 95.9

Find 37 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGAAAACGGCGTCGTTT+TGG 0.283292 8_2:-7352345 None:intergenic
GAATGTGTTCAAGGGAGCTT+TGG 0.356101 8_2:+7352893 Msa1231240:CDS
GATCCGACGGAATTTCTTCC+CGG 0.365380 8_2:+7352598 Msa1231240:CDS
GGAAGAGAGTGAAATTGAAT+TGG 0.374216 8_2:+7352675 Msa1231240:CDS
ACATTAACAACAACGTTGTT+TGG 0.375493 8_2:-7352418 None:intergenic
CGTAAAGTGTTATTCTTTGA+CGG 0.401030 8_2:-7352273 None:intergenic
GTATAATATCTAAGAGTGTT+TGG 0.411313 8_2:-7352384 None:intergenic
GAAGAAGAGGAAAGAGAAGA+AGG 0.412992 8_2:+7352775 Msa1231240:CDS
AAATTCCGTCGGATCTGAAT+TGG 0.419938 8_2:-7352590 None:intergenic
CATGTCGTTGATGGATTTGT+TGG 0.421193 8_2:+7352654 Msa1231240:CDS
GTGTGTATGGTGAGGGATAA+AGG 0.427186 8_2:+7352835 Msa1231240:CDS
AATGTGTTCAAGGGAGCTTT+GGG 0.432008 8_2:+7352894 Msa1231240:CDS
AGGAGAAGGGAGTAATGTTA+AGG 0.433410 8_2:+7352795 Msa1231240:CDS
AAAATCATGGAAAGCTTTCA+AGG 0.440126 8_2:+7352495 Msa1231240:CDS
GCAGGTTAGCATGTCGTTGA+TGG 0.473676 8_2:+7352645 Msa1231240:CDS
AGGAAAGAGAAGAAGGAGAA+GGG 0.480424 8_2:+7352782 Msa1231240:CDS
GAGGAAAGAGAAGAAGGAGA+AGG 0.500274 8_2:+7352781 Msa1231240:CDS
ACACAATTGTTGCGTGTGTA+TGG 0.501642 8_2:+7352822 Msa1231240:CDS
TTGTTAAAGATCGAAAATCA+TGG 0.528304 8_2:+7352482 Msa1231240:CDS
GCTAACCTGCGCCGTCTCAT+CGG 0.529102 8_2:-7352632 None:intergenic
TCATCGGAAAACTCGCCGCC+GGG 0.552591 8_2:-7352616 None:intergenic
AGAAGGGAGTAATGTTAAGG+TGG 0.555525 8_2:+7352798 Msa1231240:CDS
CTCATCGGAAAACTCGCCGC+CGG 0.561315 8_2:-7352617 None:intergenic
CTGCAAAACGTGTGTCCACA+TGG 0.562500 8_2:-7352871 None:intergenic
TGAACTTCTAGTGTTGCTTG+TGG 0.565142 8_2:-7352308 None:intergenic
AGTAGTGACGTTGGTGATGA+AGG 0.567789 8_2:+7352703 Msa1231240:CDS
AGGGAGCTTTGGGTTAGTAG+AGG 0.575720 8_2:+7352904 Msa1231240:CDS
AAAATAAAATGATTGCAAAG+AGG 0.605793 8_2:-7352934 None:intergenic
TCCGACCAATTCAGATCCGA+CGG 0.610865 8_2:+7352585 Msa1231240:CDS
TTGTTGCGTGTGTATGGTGA+GGG 0.665548 8_2:+7352828 Msa1231240:CDS
CCGCCGGGAAGAAATTCCGT+CGG 0.673552 8_2:-7352601 None:intergenic
TCCGTCGGATCTGAATTGGT+CGG 0.675352 8_2:-7352586 None:intergenic
ATTGTTGCGTGTGTATGGTG+AGG 0.686096 8_2:+7352827 Msa1231240:CDS
GGTGCAGCGTTTATACCATG+TGG 0.688981 8_2:+7352856 Msa1231240:CDS
TCACCAACAACAACAAAACG+AGG 0.705279 8_2:+7352558 Msa1231240:CDS
TGGTGATGAAGGTGATGACG+TGG 0.717305 8_2:+7352714 Msa1231240:CDS
TTGGAACGAAGTAGTGACGT+TGG 0.731633 8_2:+7352694 Msa1231240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATGATTGCAAAG+AGG - chr8_2:7352937-7352956 None:intergenic 20.0%
!!! GTATAATATCTAAGAGTGTT+TGG - chr8_2:7352387-7352406 None:intergenic 25.0%
! TTGTTAAAGATCGAAAATCA+TGG + chr8_2:7352482-7352501 Msa1231240:CDS 25.0%
! CGTAAAGTGTTATTCTTTGA+CGG - chr8_2:7352276-7352295 None:intergenic 30.0%
!!! GTTTTTTTGGTTGGAGAAAA+CGG - chr8_2:7352359-7352378 None:intergenic 30.0%
!!! TTGGTTTGTGTTTTTTTGGT+TGG - chr8_2:7352368-7352387 None:intergenic 30.0%
!!! GTGTTTGGTTTGTGTTTTTT+TGG - chr8_2:7352372-7352391 None:intergenic 30.0%
ACATTAACAACAACGTTGTT+TGG - chr8_2:7352421-7352440 None:intergenic 30.0%
! GTTATTGTTGTTGTTGTTGT+CGG - chr8_2:7352452-7352471 None:intergenic 30.0%
AAAATCATGGAAAGCTTTCA+AGG + chr8_2:7352495-7352514 Msa1231240:CDS 30.0%
AGAAGAAGAAAAAGAAGAAG+AGG + chr8_2:7352762-7352781 Msa1231240:CDS 30.0%
!!! TATCCTCGTTTTGTTGTTGT+TGG - chr8_2:7352564-7352583 None:intergenic 35.0%
GGAAGAGAGTGAAATTGAAT+TGG + chr8_2:7352675-7352694 Msa1231240:CDS 35.0%
! GTTTTGCAGAATGTGTTCAA+GGG + chr8_2:7352885-7352904 Msa1231240:CDS 35.0%
!! TGAACTTCTAGTGTTGCTTG+TGG - chr8_2:7352311-7352330 None:intergenic 40.0%
TCACCAACAACAACAAAACG+AGG + chr8_2:7352558-7352577 Msa1231240:CDS 40.0%
AAATTCCGTCGGATCTGAAT+TGG - chr8_2:7352593-7352612 None:intergenic 40.0%
!! CATGTCGTTGATGGATTTGT+TGG + chr8_2:7352654-7352673 Msa1231240:CDS 40.0%
GAAGAAGAGGAAAGAGAAGA+AGG + chr8_2:7352775-7352794 Msa1231240:CDS 40.0%
AGGAAAGAGAAGAAGGAGAA+GGG + chr8_2:7352782-7352801 Msa1231240:CDS 40.0%
AGGAGAAGGGAGTAATGTTA+AGG + chr8_2:7352795-7352814 Msa1231240:CDS 40.0%
AGAAGGGAGTAATGTTAAGG+TGG + chr8_2:7352798-7352817 Msa1231240:CDS 40.0%
ACACAATTGTTGCGTGTGTA+TGG + chr8_2:7352822-7352841 Msa1231240:CDS 40.0%
! CGTTTTGCAGAATGTGTTCA+AGG + chr8_2:7352884-7352903 Msa1231240:CDS 40.0%
AATGTGTTCAAGGGAGCTTT+GGG + chr8_2:7352894-7352913 Msa1231240:CDS 40.0%
TTGGAACGAAGTAGTGACGT+TGG + chr8_2:7352694-7352713 Msa1231240:CDS 45.0%
!! AGTAGTGACGTTGGTGATGA+AGG + chr8_2:7352703-7352722 Msa1231240:CDS 45.0%
GAGGAAAGAGAAGAAGGAGA+AGG + chr8_2:7352781-7352800 Msa1231240:CDS 45.0%
ATTGTTGCGTGTGTATGGTG+AGG + chr8_2:7352827-7352846 Msa1231240:CDS 45.0%
TTGTTGCGTGTGTATGGTGA+GGG + chr8_2:7352828-7352847 Msa1231240:CDS 45.0%
GTGTGTATGGTGAGGGATAA+AGG + chr8_2:7352835-7352854 Msa1231240:CDS 45.0%
GAATGTGTTCAAGGGAGCTT+TGG + chr8_2:7352893-7352912 Msa1231240:CDS 45.0%
! TGGAGAAAACGGCGTCGTTT+TGG - chr8_2:7352348-7352367 None:intergenic 50.0%
TCCGACCAATTCAGATCCGA+CGG + chr8_2:7352585-7352604 Msa1231240:CDS 50.0%
TCCGTCGGATCTGAATTGGT+CGG - chr8_2:7352589-7352608 None:intergenic 50.0%
GATCCGACGGAATTTCTTCC+CGG + chr8_2:7352598-7352617 Msa1231240:CDS 50.0%
GCAGGTTAGCATGTCGTTGA+TGG + chr8_2:7352645-7352664 Msa1231240:CDS 50.0%
! TGGTGATGAAGGTGATGACG+TGG + chr8_2:7352714-7352733 Msa1231240:CDS 50.0%
GGTGCAGCGTTTATACCATG+TGG + chr8_2:7352856-7352875 Msa1231240:CDS 50.0%
CTGCAAAACGTGTGTCCACA+TGG - chr8_2:7352874-7352893 None:intergenic 50.0%
!! AGGGAGCTTTGGGTTAGTAG+AGG + chr8_2:7352904-7352923 Msa1231240:CDS 50.0%
! CGGCGAGTTTTCCGATGAGA+CGG + chr8_2:7352621-7352640 Msa1231240:CDS 55.0%
CCGCCGGGAAGAAATTCCGT+CGG - chr8_2:7352604-7352623 None:intergenic 60.0%
CCGACGGAATTTCTTCCCGG+CGG + chr8_2:7352601-7352620 Msa1231240:CDS 60.0%
TCATCGGAAAACTCGCCGCC+GGG - chr8_2:7352619-7352638 None:intergenic 60.0%
CTCATCGGAAAACTCGCCGC+CGG - chr8_2:7352620-7352639 None:intergenic 60.0%
! GTTTTCCGATGAGACGGCGC+AGG + chr8_2:7352627-7352646 Msa1231240:CDS 60.0%
GCTAACCTGCGCCGTCTCAT+CGG - chr8_2:7352635-7352654 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 7352265 7352978 7352265 ID=Msa1231240;Name=Msa1231240
chr8_2 mRNA 7352265 7352978 7352265 ID=Msa1231240-mRNA-1;Parent=Msa1231240;Name=Msa1231240-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|237
chr8_2 exon 7352265 7352978 7352265 ID=Msa1231240-mRNA-1:exon:2748;Parent=Msa1231240-mRNA-1
chr8_2 CDS 7352265 7352978 7352265 ID=Msa1231240-mRNA-1:cds;Parent=Msa1231240-mRNA-1
Gene Sequence

>Msa1231240

ATGGAAAACCGTCAAAGAATAACACTTTACGATCAAATGACATCCACAAGCAACACTAGAAGTTCACTAGCTAGTTTAATCCAAAACGACGCCGTTTTCTCCAACCAAAAAAACACAAACCAAACACTCTTAGATATTATACGAGAAAACGAACCAAACAACGTTGTTGTTAATGTTAGTGTTACCGACAACAACAACAACAATAACAGCAACAACGTTGTTAAAGATCGAAAATCATGGAAAGCTTTCAAGGAATTGCTTCTTTTCAAACGTCGTAACTCTGAAGAATCAACTCACCAACAACAACAAAACGAGGATATTCCGACCAATTCAGATCCGACGGAATTTCTTCCCGGCGGCGAGTTTTCCGATGAGACGGCGCAGGTTAGCATGTCGTTGATGGATTTGTTGGAAGAGAGTGAAATTGAATTGGAACGAAGTAGTGACGTTGGTGATGAAGGTGATGACGTGGAAGAGAATAATGAGAAACGAGAAGAAGAAGAAGAAAAAGAAGAAGAGGAAAGAGAAGAAGGAGAAGGGAGTAATGTTAAGGTGGAACACAATTGTTGCGTGTGTATGGTGAGGGATAAAGGTGCAGCGTTTATACCATGTGGACACACGTTTTGCAGAATGTGTTCAAGGGAGCTTTGGGTTAGTAGAGGAAATTGTCCTCTTTGCAATCATTTTATTTTAGAAGTTCTTGATATTTTCTGA

Protein sequence

>Msa1231240

MENRQRITLYDQMTSTSNTRSSLASLIQNDAVFSNQKNTNQTLLDIIRENEPNNVVVNVSVTDNNNNNNSNNVVKDRKSWKAFKELLLFKRRNSEESTHQQQQNEDIPTNSDPTEFLPGGEFSDETAQVSMSLMDLLEESEIELERSSDVGDEGDDVEENNEKREEEEEKEEEEREEGEGSNVKVEHNCCVCMVRDKGAAFIPCGHTFCRMCSRELWVSRGNCPLCNHFILEVLDIF*