AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625070 MtrunA17_Chr4g0000551 76.056 71 14 1 8 78 1 68 1.28e-33 110
Msa0625070 MtrunA17_Chr5g0417121 60.000 65 23 2 1 65 1 62 7.33e-21 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625070 AT1G51650.1 50.000 68 29 2 8 75 7 69 2.32e-18 72.0

Find 17 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGACAGCCAATCTTATT+AGG 0.245215 4_3:+4072440 Msa0625070:CDS
GCAGAGGAAGAGTAGCGATT+TGG 0.385613 4_3:+4072383 Msa0625070:CDS
AAGGCAATTCCTAATAAGAT+TGG 0.392366 4_3:-4072449 None:intergenic
TCTTATTAGGAATTGCCTTA+AGG 0.405336 4_3:+4072453 Msa0625070:CDS
GTAGCGATTTGGAGAGCAGC+AGG 0.455679 4_3:+4072394 Msa0625070:CDS
AATAAGATTGGCTGTCAAGT+TGG 0.465236 4_3:-4072437 None:intergenic
GTAACGAAAACAAGTGTAAG+AGG 0.479461 4_3:+4072308 Msa0625070:five_prime_UTR
ACATGGATCGACAGTCATTC+CGG 0.483118 4_3:+4072535 Msa0625070:CDS
CGATCAATGGCGTCAGGCAG+AGG 0.495109 4_3:+4072367 Msa0625070:exon
GATAATCGATCAATGGCGTC+AGG 0.508621 4_3:+4072361 Msa0625070:exon
CGTATCAGATAATCGATCAA+TGG 0.522049 4_3:+4072354 Msa0625070:exon
TTCAGTACTTGCTTCCCACA+TGG 0.547985 4_3:+4072518 Msa0625070:CDS
AGCAAATATTATTCTCGACA+TGG 0.585822 4_3:+4074975 Msa0625070:intron
TAGCGATTTGGAGAGCAGCA+GGG 0.642009 4_3:+4072395 Msa0625070:CDS
GAATGACTGTCGATCCATGT+GGG 0.656324 4_3:-4072532 None:intergenic
GGAATGACTGTCGATCCATG+TGG 0.711549 4_3:-4072533 None:intergenic
GCAAGAAGCATTAACCCACG+CGG 0.807767 4_3:+4075002 Msa0625070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATTTTCTTAAGTTATTA+CGG + chr4_3:4073037-4073056 Msa0625070:intron 15.0%
!!! ATTGTTTTCATTATTATTAG+TGG + chr4_3:4073312-4073331 Msa0625070:intron 15.0%
!!! ATTTTTCTTTGGAAAATAAT+TGG + chr4_3:4074047-4074066 Msa0625070:intron 15.0%
!!! ATTTGAATGTGTTTTTTAAA+GGG - chr4_3:4074167-4074186 None:intergenic 15.0%
!! TATACAAAACTAAATAAACT+TGG - chr4_3:4074623-4074642 None:intergenic 15.0%
!!! TTTTTTTCTTTCTAAGAACT+CGG - chr4_3:4072716-4072735 None:intergenic 20.0%
!! CCTATCATCTTAATTAATTA+GGG - chr4_3:4072904-4072923 None:intergenic 20.0%
!! CCTAATTAATTAAGATGATA+GGG + chr4_3:4072902-4072921 Msa0625070:intron 20.0%
!!! TCATTTTCTTAAGTTATTAC+GGG + chr4_3:4073038-4073057 Msa0625070:intron 20.0%
!!! ATTTTGTCTCAAAAATATCA+TGG - chr4_3:4073448-4073467 None:intergenic 20.0%
!!! CAATTTAGTCTAAAATATCA+TGG - chr4_3:4073568-4073587 None:intergenic 20.0%
!!! ATATTTTAGACTAAATTGAG+AGG + chr4_3:4073570-4073589 Msa0625070:intron 20.0%
!! TCAATTAAACAGAAATGAAA+TGG - chr4_3:4073807-4073826 None:intergenic 20.0%
!! TTCATTTCTGTTTAATTGAT+TGG + chr4_3:4073808-4073827 Msa0625070:intron 20.0%
!!! TTTTGGTAAAGATTTTTCTT+TGG + chr4_3:4074036-4074055 Msa0625070:intron 20.0%
!!! AGAATTTTATCGCTTTTAAT+CGG - chr4_3:4074094-4074113 None:intergenic 20.0%
!!! CATTTGAATGTGTTTTTTAA+AGG - chr4_3:4074168-4074187 None:intergenic 20.0%
!!! TTTAATGTGATGTTTTCATT+AGG + chr4_3:4074313-4074332 Msa0625070:intron 20.0%
!! ATCATAACCAAAAAAGAAAA+CGG - chr4_3:4074818-4074837 None:intergenic 20.0%
!!! ATCTTGTTTGTAATAATTTG+AGG + chr4_3:4074876-4074895 Msa0625070:intron 20.0%
! CCATAATGTTAACTTTCTTA+GGG + chr4_3:4072809-4072828 Msa0625070:intron 25.0%
! CCCTAATTAATTAAGATGAT+AGG + chr4_3:4072901-4072920 Msa0625070:intron 25.0%
! CCCTATCATCTTAATTAATT+AGG - chr4_3:4072905-4072924 None:intergenic 25.0%
!!! GAACACATTTTTTAGATTGA+AGG + chr4_3:4072942-4072961 Msa0625070:intron 25.0%
! CTTTAGTATCTTGTCTATAT+CGG + chr4_3:4073073-4073092 Msa0625070:intron 25.0%
! AGATGATTTAGAAACTACTT+TGG + chr4_3:4073133-4073152 Msa0625070:intron 25.0%
!!! CATTTTCATTGATTTCTGAA+AGG - chr4_3:4073284-4073303 None:intergenic 25.0%
! TCATTTAATTACCTAACTTG+TGG + chr4_3:4073643-4073662 Msa0625070:intron 25.0%
! TTTGTGCTTTATGAAGAAAA+GGG - chr4_3:4073717-4073736 None:intergenic 25.0%
!! TTTTGTGCTTTATGAAGAAA+AGG - chr4_3:4073718-4073737 None:intergenic 25.0%
!!! CTTTTTCAAACTTTTGTCAT+TGG + chr4_3:4073850-4073869 Msa0625070:intron 25.0%
!!! CAATGAGTTTTGCTTTAAAT+TGG + chr4_3:4074114-4074133 Msa0625070:intron 25.0%
! ATTTCACTTCGAATCATAAT+TGG + chr4_3:4074211-4074230 Msa0625070:intron 25.0%
! TATTGATCTTAATCAGTTTG+AGG - chr4_3:4074250-4074269 None:intergenic 25.0%
! ATGAAAACATCACATTAAAG+TGG - chr4_3:4074312-4074331 None:intergenic 25.0%
!! ATACATCTAAAACTCTAGAA+AGG - chr4_3:4074794-4074813 None:intergenic 25.0%
!! ATGTATTCCGTTTTCTTTTT+TGG + chr4_3:4074808-4074827 Msa0625070:intron 25.0%
AAGGCAATTCCTAATAAGAT+TGG - chr4_3:4072452-4072471 None:intergenic 30.0%
TCTTATTAGGAATTGCCTTA+AGG + chr4_3:4072453-4072472 Msa0625070:CDS 30.0%
GGTTTCAAACTATTGAGTAT+TGG + chr4_3:4072657-4072676 Msa0625070:intron 30.0%
GCCATAATGTTAACTTTCTT+AGG + chr4_3:4072808-4072827 Msa0625070:intron 30.0%
CCCTAAGAAAGTTAACATTA+TGG - chr4_3:4072812-4072831 None:intergenic 30.0%
! CAAGCTTTGTAGAAACAAAT+GGG - chr4_3:4072849-4072868 None:intergenic 30.0%
! TCAAGCTTTGTAGAAACAAA+TGG - chr4_3:4072850-4072869 None:intergenic 30.0%
!! ATTTGTTTCTACAAAGCTTG+AGG + chr4_3:4072849-4072868 Msa0625070:intron 30.0%
!! TTTGTTTCTACAAAGCTTGA+GGG + chr4_3:4072850-4072869 Msa0625070:intron 30.0%
GAAGATAAAAATGCTAGCTA+GGG - chr4_3:4073349-4073368 None:intergenic 30.0%
AGAAGATAAAAATGCTAGCT+AGG - chr4_3:4073350-4073369 None:intergenic 30.0%
! GAGAGGTTATTCTAAAGAAA+TGG + chr4_3:4073587-4073606 Msa0625070:intron 30.0%
AAAGGATTATCTCAGAACAT+GGG - chr4_3:4073741-4073760 None:intergenic 30.0%
AAAAGGATTATCTCAGAACA+TGG - chr4_3:4073742-4073761 None:intergenic 30.0%
! GATAATCCTTTTCTGTTTAG+TGG + chr4_3:4073750-4073769 Msa0625070:intron 30.0%
!!! CTTTTAATCGGTTTGAAGTT+TGG - chr4_3:4074082-4074101 None:intergenic 30.0%
! AAATGACACTTACGTGATTT+TGG + chr4_3:4074183-4074202 Msa0625070:intron 30.0%
! ATCTTAATCAGTTTGAGGTT+TGG - chr4_3:4074245-4074264 None:intergenic 30.0%
! CCAATTTGAAGTAAAACTCA+AGG - chr4_3:4074281-4074300 None:intergenic 30.0%
! CCTTGAGTTTTACTTCAAAT+TGG + chr4_3:4074278-4074297 Msa0625070:intron 30.0%
!!! TACAAGTGTACCTCTTTTTT+TGG - chr4_3:4074465-4074484 None:intergenic 30.0%
! TAAAAGAGGTACACTTGTTA+GGG + chr4_3:4074483-4074502 Msa0625070:intron 30.0%
!!! TCTTTTTTGGTTATGATGTG+TGG + chr4_3:4074821-4074840 Msa0625070:intron 30.0%
AGATGATAATATCCCTTCAT+AGG + chr4_3:4074922-4074941 Msa0625070:intron 30.0%
AGCAAATATTATTCTCGACA+TGG + chr4_3:4074975-4074994 Msa0625070:intron 30.0%
GTAACGAAAACAAGTGTAAG+AGG + chr4_3:4072308-4072327 Msa0625070:five_prime_UTR 35.0%
CGTATCAGATAATCGATCAA+TGG + chr4_3:4072354-4072373 Msa0625070:exon 35.0%
! AATAAGATTGGCTGTCAAGT+TGG - chr4_3:4072440-4072459 None:intergenic 35.0%
ACTTGACAGCCAATCTTATT+AGG + chr4_3:4072440-4072459 Msa0625070:CDS 35.0%
!! GAGAGAATTTGAGTTTTGTG+AGG - chr4_3:4072481-4072500 None:intergenic 35.0%
AGAAGAGAGAAAGAGAAAGA+GGG - chr4_3:4072577-4072596 None:intergenic 35.0%
AAGAAGAGAGAAAGAGAAAG+AGG - chr4_3:4072578-4072597 None:intergenic 35.0%
GCTTAAACAATGTCTCTTGT+TGG + chr4_3:4072768-4072787 Msa0625070:intron 35.0%
TCTTGTTGGAACTTGAGTTT+GGG + chr4_3:4072782-4072801 Msa0625070:intron 35.0%
!! TTTTAGATTGAAGGTGTGAG+TGG + chr4_3:4072951-4072970 Msa0625070:intron 35.0%
!! ACACACATATTTTGTTGTGC+CGG - chr4_3:4073003-4073022 None:intergenic 35.0%
CATCTCAAAATTTGTCCCAT+AGG - chr4_3:4073118-4073137 None:intergenic 35.0%
! ACTACTTTGGAGAGAAATTG+AGG + chr4_3:4073146-4073165 Msa0625070:intron 35.0%
! CTACTTTGGAGAGAAATTGA+GGG + chr4_3:4073147-4073166 Msa0625070:intron 35.0%
AATACTCCACTTCACTTCAT+CGG - chr4_3:4073255-4073274 None:intergenic 35.0%
! TTCTAAAGAAATGGTGAGTG+TGG + chr4_3:4073596-4073615 Msa0625070:intron 35.0%
TCATTGCCACTAAACAGAAA+AGG - chr4_3:4073759-4073778 None:intergenic 35.0%
AGAAATGAAATGGAAACGCA+GGG - chr4_3:4073797-4073816 None:intergenic 35.0%
TCGACAAATAGATGTTACAC+AGG - chr4_3:4073954-4073973 None:intergenic 35.0%
ACATTCAACTCACACTTACA+TGG + chr4_3:4074013-4074032 Msa0625070:intron 35.0%
! AACTCACACTTACATGGTTT+TGG + chr4_3:4074019-4074038 Msa0625070:intron 35.0%
ACTGTGTGAAACTGTGTTTA+AGG - chr4_3:4074415-4074434 None:intergenic 35.0%
CACTTAGAAGCCAAAAAAAG+AGG + chr4_3:4074452-4074471 Msa0625070:intron 35.0%
! AAGAGGTACACTTGTAAAAG+AGG + chr4_3:4074469-4074488 Msa0625070:intron 35.0%
! GTAAAAGAGGTACACTTGTT+AGG + chr4_3:4074482-4074501 Msa0625070:intron 35.0%
TTAGGGATCTAAGGAGAAAA+GGG + chr4_3:4074500-4074519 Msa0625070:intron 35.0%
CCATGCAAATCCAAAATTCA+GGG - chr4_3:4074853-4074872 None:intergenic 35.0%
ACCATGCAAATCCAAAATTC+AGG - chr4_3:4074854-4074873 None:intergenic 35.0%
AGTGAGATTCATCCTATGAA+GGG - chr4_3:4074937-4074956 None:intergenic 35.0%
AAGTGAGATTCATCCTATGA+AGG - chr4_3:4074938-4074957 None:intergenic 35.0%
!! GAGTTTTGTGAGGTTCCTTA+AGG - chr4_3:4072471-4072490 None:intergenic 40.0%
AAGAGAGAAAGAGAAAGAGG+GGG - chr4_3:4072575-4072594 None:intergenic 40.0%
GAAGAGAGAAAGAGAAAGAG+GGG - chr4_3:4072576-4072595 None:intergenic 40.0%
TTTACCCTTCCTTCATCACT+TGG + chr4_3:4072617-4072636 Msa0625070:intron 40.0%
TTACCCTTCCTTCATCACTT+GGG + chr4_3:4072618-4072637 Msa0625070:intron 40.0%
CTCTTGTTGGAACTTGAGTT+TGG + chr4_3:4072781-4072800 Msa0625070:intron 40.0%
GCTTTGTAGAAACAAATGGG+TGG - chr4_3:4072846-4072865 None:intergenic 40.0%
! TATCGGTGCTTCATAGATAG+AGG + chr4_3:4073090-4073109 Msa0625070:intron 40.0%
! ATCGGTGCTTCATAGATAGA+GGG + chr4_3:4073091-4073110 Msa0625070:intron 40.0%
TTCATAGATAGAGGGACCTA+TGG + chr4_3:4073099-4073118 Msa0625070:intron 40.0%
TCATAGATAGAGGGACCTAT+GGG + chr4_3:4073100-4073119 Msa0625070:intron 40.0%
AAGAAACCGATGAAGTGAAG+TGG + chr4_3:4073246-4073265 Msa0625070:intron 40.0%
!! AAATTCTGGTGCCACAAGTT+AGG - chr4_3:4073657-4073676 None:intergenic 40.0%
!! AGCGATGAGTACTGAAATTC+TGG - chr4_3:4073671-4073690 None:intergenic 40.0%
TTCAGTACTCATCGCTACAA+TGG + chr4_3:4073675-4073694 Msa0625070:intron 40.0%
TACTCATCGCTACAATGGTT+AGG + chr4_3:4073680-4073699 Msa0625070:intron 40.0%
CAGAAATGAAATGGAAACGC+AGG - chr4_3:4073798-4073817 None:intergenic 40.0%
AGGGTAACTTCACATTACAG+TGG - chr4_3:4074148-4074167 None:intergenic 40.0%
! GTACACTTGTTAGGGATCTA+AGG + chr4_3:4074491-4074510 Msa0625070:intron 40.0%
GTTAGGGATCTAAGGAGAAA+AGG + chr4_3:4074499-4074518 Msa0625070:intron 40.0%
GGCGTTAAATGCAAATCATC+AGG - chr4_3:4074602-4074621 None:intergenic 40.0%
TCACTGAAACGCATTCATGA+TGG + chr4_3:4074726-4074745 Msa0625070:intron 40.0%
! CCCTGAATTTTGGATTTGCA+TGG + chr4_3:4074850-4074869 Msa0625070:intron 40.0%
GATAATCGATCAATGGCGTC+AGG + chr4_3:4072361-4072380 Msa0625070:exon 45.0%
TTCAGTACTTGCTTCCCACA+TGG + chr4_3:4072518-4072537 Msa0625070:CDS 45.0%
GAATGACTGTCGATCCATGT+GGG - chr4_3:4072535-4072554 None:intergenic 45.0%
ACATGGATCGACAGTCATTC+CGG + chr4_3:4072535-4072554 Msa0625070:CDS 45.0%
CAACCCAAGTGATGAAGGAA+GGG - chr4_3:4072624-4072643 None:intergenic 45.0%
TCAACCCAAGTGATGAAGGA+AGG - chr4_3:4072625-4072644 None:intergenic 45.0%
TAGGTCAACCCAAGTGATGA+AGG - chr4_3:4072629-4072648 None:intergenic 45.0%
!! TTCATCACTTGGGTTGACCT+AGG + chr4_3:4072628-4072647 Msa0625070:intron 45.0%
! AGTTTGAAACCCCAAGACCT+AGG - chr4_3:4072648-4072667 None:intergenic 45.0%
!! CTATTGAGTATTGGAGCGCT+TGG + chr4_3:4072666-4072685 Msa0625070:intron 45.0%
! ATTGGAGCGCTTGGATTAAC+CGG + chr4_3:4072675-4072694 Msa0625070:intron 45.0%
! TTTCTAAGAACTCGGCATCC+GGG - chr4_3:4072708-4072727 None:intergenic 45.0%
! CTTTCTAAGAACTCGGCATC+CGG - chr4_3:4072709-4072728 None:intergenic 45.0%
GAGTGGTGTCTAACATGTGT+TGG + chr4_3:4072968-4072987 Msa0625070:intron 45.0%
AGAAATGGTGAGTGTGGAGT+AGG + chr4_3:4073602-4073621 Msa0625070:intron 45.0%
TGTGGAGTAGGAACTAGGAA+TGG + chr4_3:4073614-4073633 Msa0625070:intron 45.0%
TGAAATGGAAACGCAGGGAA+GGG - chr4_3:4073792-4073811 None:intergenic 45.0%
ATGAAATGGAAACGCAGGGA+AGG - chr4_3:4073793-4073812 None:intergenic 45.0%
!! CTCTGGGATGTCTGTATGAA+TGG - chr4_3:4074692-4074711 None:intergenic 45.0%
CATTCATACAGACATCCCAG+AGG + chr4_3:4074690-4074709 Msa0625070:intron 45.0%
! GAAGCTAATCTGAGATCTGC+TGG + chr4_3:4074748-4074767 Msa0625070:intron 45.0%
GCAGAGGAAGAGTAGCGATT+TGG + chr4_3:4072383-4072402 Msa0625070:CDS 50.0%
! TAGCGATTTGGAGAGCAGCA+GGG + chr4_3:4072395-4072414 Msa0625070:CDS 50.0%
GGAATGACTGTCGATCCATG+TGG - chr4_3:4072536-4072555 None:intergenic 50.0%
! GGGGGTCTTACTGAGTTTTC+CGG - chr4_3:4072557-4072576 None:intergenic 50.0%
!! ACTTGGGTTGACCTAGGTCT+TGG + chr4_3:4072634-4072653 Msa0625070:intron 50.0%
!! CTTGGGTTGACCTAGGTCTT+GGG + chr4_3:4072635-4072654 Msa0625070:intron 50.0%
!! TTGGGTTGACCTAGGTCTTG+GGG + chr4_3:4072636-4072655 Msa0625070:intron 50.0%
! TTCTAAGAACTCGGCATCCG+GGG - chr4_3:4072707-4072726 None:intergenic 50.0%
GTGAGTGTGGAGTAGGAACT+AGG + chr4_3:4073609-4073628 Msa0625070:intron 50.0%
GGGATCTAAGGAGAAAAGGG+TGG + chr4_3:4074503-4074522 Msa0625070:intron 50.0%
GACGTCGTCTTGATTCCTCT+GGG - chr4_3:4074708-4074727 None:intergenic 50.0%
TGACGTCGTCTTGATTCCTC+TGG - chr4_3:4074709-4074728 None:intergenic 50.0%
! GTGGCAGATGCCCTGAATTT+TGG + chr4_3:4074840-4074859 Msa0625070:intron 50.0%
! GTAGCGATTTGGAGAGCAGC+AGG + chr4_3:4072394-4072413 Msa0625070:CDS 55.0%
GGATTAACCGGTCCTTTCCC+CGG + chr4_3:4072687-4072706 Msa0625070:intron 55.0%
! CATGTGTTGGTGTGCGACAC+CGG + chr4_3:4072981-4073000 Msa0625070:intron 55.0%
CGATCAATGGCGTCAGGCAG+AGG + chr4_3:4072367-4072386 Msa0625070:exon 60.0%
AGAACTCGGCATCCGGGGAA+AGG - chr4_3:4072702-4072721 None:intergenic 60.0%
! AACGCAGGGAAGGGTGTGAG+TGG - chr4_3:4073783-4073802 None:intergenic 60.0%
TCGGCATCCGGGGAAAGGAC+CGG - chr4_3:4072697-4072716 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 4072297 4075023 4072297 ID=Msa0625070;Name=Msa0625070
chr4_3 mRNA 4072297 4075023 4072297 ID=Msa0625070-mRNA-1;Parent=Msa0625070;Name=Msa0625070-mRNA-1;_AED=0.30;_eAED=0.30;_QI=76|0|0.5|0.5|1|1|2|0|80
chr4_3 exon 4072297 4072565 4072297 ID=Msa0625070-mRNA-1:exon:955;Parent=Msa0625070-mRNA-1
chr4_3 exon 4074977 4075023 4074977 ID=Msa0625070-mRNA-1:exon:956;Parent=Msa0625070-mRNA-1
chr4_3 five_prime_UTR 4072297 4072372 4072297 ID=Msa0625070-mRNA-1:five_prime_utr;Parent=Msa0625070-mRNA-1
chr4_3 CDS 4072373 4072565 4072373 ID=Msa0625070-mRNA-1:cds;Parent=Msa0625070-mRNA-1
chr4_3 CDS 4074977 4075023 4074977 ID=Msa0625070-mRNA-1:cds;Parent=Msa0625070-mRNA-1
Gene Sequence

>Msa0625070

ATGGCGTCAGGCAGAGGAAGAGTAGCGATTTGGAGAGCAGCAGGGATGACATACACAGCATATTCCAACTTGACAGCCAATCTTATTAGGAATTGCCTTAAGGAACCTCACAAAACTCAAATTCTCTCTCTTTCTCGCGAGAACGTTCAGTACTTGCTTCCCACATGGATCGACAGTCATTCCGGAAAACTCACAAATATTATTCTCGACATGGAAATGCAAGAAGCATTAACCCACGCG

Protein sequence

>Msa0625070

MASGRGRVAIWRAAGMTYTAYSNLTANLIRNCLKEPHKTQILSLSRENVQYLLPTWIDSHSGKLTNIILDMEMQEALTHA