Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212080 | XP_013464264.1 | 94.828 | 116 | 6 | 0 | 1 | 116 | 1 | 116 | 3.96e-78 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212080 | sp|Q8LC53|EPF2_ARATH | 47.222 | 108 | 51 | 2 | 13 | 115 | 12 | 118 | 5.85e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212080 | A0A072V8D7 | 94.828 | 116 | 6 | 0 | 1 | 116 | 1 | 116 | 1.89e-78 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0019600 | Msa0212080 | 0.872894 | 2.238954e-67 | -8.615850e-47 |
| Msa0066320 | Msa0212080 | 0.844828 | 5.935576e-59 | -8.615850e-47 |
| Msa0111750 | Msa0212080 | 0.814286 | 1.664165e-51 | -8.615850e-47 |
| Msa0124130 | Msa0212080 | 0.809759 | 1.614294e-50 | -8.615850e-47 |
| Msa0212080 | Msa0215640 | 0.806003 | 1.016009e-49 | -8.615850e-47 |
| Msa0212080 | Msa0219190 | 0.808413 | 3.135142e-50 | -8.615850e-47 |
| Msa0212080 | Msa0252630 | 0.957765 | 1.227970e-115 | -8.615850e-47 |
| Msa0212080 | Msa0291330 | 0.918913 | 8.654838e-87 | -8.615850e-47 |
| Msa0212080 | Msa0298240 | 0.818212 | 2.205243e-52 | -8.615850e-47 |
| Msa0212080 | Msa0306120 | 0.806066 | 9.850063e-50 | -8.615850e-47 |
| Msa0212080 | Msa0333700 | 0.810664 | 1.029988e-50 | -8.615850e-47 |
| Msa0212080 | Msa0343660 | 0.810165 | 1.320053e-50 | -8.615850e-47 |
| Msa0212080 | Msa0344570 | 0.836933 | 6.996281e-57 | -8.615850e-47 |
| Msa0212080 | Msa0351770 | 0.824611 | 7.350672e-54 | -8.615850e-47 |
| Msa0212080 | Msa0359250 | 0.862500 | 4.850209e-64 | -8.615850e-47 |
| Msa0212080 | Msa0375680 | 0.810772 | 9.761254e-51 | -8.615850e-47 |
| Msa0212080 | Msa0375700 | 0.814659 | 1.376446e-51 | -8.615850e-47 |
| Msa0212080 | Msa0375910 | 0.805545 | 1.267740e-49 | -8.615850e-47 |
| Msa0212080 | Msa0392420 | 0.834962 | 2.212283e-56 | -8.615850e-47 |
| Msa0212080 | Msa0392460 | 0.827315 | 1.674500e-54 | -8.615850e-47 |
| Msa0212080 | Msa0392490 | 0.836616 | 8.432707e-57 | -8.615850e-47 |
| Msa0212080 | Msa0406000 | 0.861376 | 1.071667e-63 | -8.615850e-47 |
| Msa0212080 | Msa0415320 | 0.822086 | 2.859623e-53 | -8.615850e-47 |
| Msa0212080 | Msa0424330 | 0.835569 | 1.554698e-56 | -8.615850e-47 |
| Msa0212080 | Msa0424340 | 0.821186 | 4.615801e-53 | -8.615850e-47 |
| Msa0212080 | Msa0424350 | 0.830954 | 2.195749e-55 | -8.615850e-47 |
| Msa0212080 | Msa0441590 | 0.808195 | 3.489950e-50 | -8.615850e-47 |
| Msa0212080 | Msa0451400 | 0.874207 | 8.088407e-68 | -8.615850e-47 |
| Msa0212080 | Msa0479540 | 0.822722 | 2.034203e-53 | -8.615850e-47 |
| Msa0212080 | Msa0487570 | 0.839913 | 1.192504e-57 | -8.615850e-47 |
| Msa0212080 | Msa0487630 | 0.834514 | 2.868863e-56 | -8.615850e-47 |
| Msa0212080 | Msa0492190 | 0.842881 | 1.972494e-58 | -8.615850e-47 |
| Msa0212080 | Msa0515950 | 0.825886 | 3.669661e-54 | -8.615850e-47 |
| Msa0212080 | Msa0523000 | 0.806072 | 9.821979e-50 | -8.615850e-47 |
| Msa0212080 | Msa0534930 | 0.847093 | 1.438149e-59 | -8.615850e-47 |
| Msa0212080 | Msa0535040 | 0.817261 | 3.613926e-52 | -8.615850e-47 |
| Msa0212080 | Msa0575740 | 0.814045 | 1.881409e-51 | -8.615850e-47 |
| Msa0212080 | Msa0591790 | 0.851691 | 7.523397e-61 | -8.615850e-47 |
| Msa0212080 | Msa0594090 | 0.818641 | 1.763136e-52 | -8.615850e-47 |
| Msa0212080 | Msa0669840 | 0.883438 | 4.486626e-71 | -8.615850e-47 |
| Msa0212080 | Msa0739580 | 0.823404 | 1.410433e-53 | -8.615850e-47 |
| Msa0212080 | Msa0751890 | 0.810958 | 8.897678e-51 | -8.615850e-47 |
| Msa0212080 | Msa0752840 | 0.810102 | 1.362008e-50 | -8.615850e-47 |
| Msa0212080 | Msa0764820 | 0.818742 | 1.672526e-52 | -8.615850e-47 |
| Msa0212080 | Msa0770290 | 0.805835 | 1.101791e-49 | -8.615850e-47 |
| Msa0212080 | Msa0782360 | 0.811919 | 5.503150e-51 | -8.615850e-47 |
| Msa0212080 | Msa0819890 | 0.807117 | 5.912643e-50 | -8.615850e-47 |
| Msa0212080 | Msa0831940 | 0.804474 | 2.123023e-49 | -8.615850e-47 |
| Msa0212080 | Msa0849280 | 0.818112 | 2.323592e-52 | -8.615850e-47 |
| Msa0212080 | Msa0851020 | 0.822566 | 2.212533e-53 | -8.615850e-47 |
| Msa0212080 | Msa0860280 | 0.817547 | 3.116329e-52 | -8.615850e-47 |
| Msa0212080 | Msa0867350 | 0.833626 | 4.787179e-56 | -8.615850e-47 |
| Msa0212080 | Msa0898730 | 0.827919 | 1.198703e-54 | -8.615850e-47 |
| Msa0212080 | Msa0916640 | 0.800027 | 1.746896e-48 | -8.615850e-47 |
| Msa0212080 | Msa0929870 | 0.838052 | 3.614943e-57 | -8.615850e-47 |
| Msa0212080 | Msa0953540 | 0.811620 | 6.392578e-51 | -8.615850e-47 |
| Msa0212080 | Msa0961560 | 0.800685 | 1.283292e-48 | -8.615850e-47 |
| Msa0212080 | Msa0996270 | 0.804910 | 1.721422e-49 | -8.615850e-47 |
| Msa0212080 | Msa1037890 | 0.834818 | 2.405410e-56 | -8.615850e-47 |
| Msa0212080 | Msa1037900 | 0.843432 | 1.406435e-58 | -8.615850e-47 |
| Msa0212080 | Msa1037910 | 0.839069 | 1.975196e-57 | -8.615850e-47 |
| Msa0212080 | Msa1081570 | 0.809347 | 1.978925e-50 | -8.615850e-47 |
| Msa0212080 | Msa1084520 | 0.813786 | 2.146218e-51 | -8.615850e-47 |
| Msa0212080 | Msa1125810 | 0.814436 | 1.542464e-51 | -8.615850e-47 |
| Msa0212080 | Msa1127140 | 0.811333 | 7.376540e-51 | -8.615850e-47 |
| Msa0212080 | Msa1129950 | 0.840247 | 9.754695e-58 | -8.615850e-47 |
| Msa0212080 | Msa1129960 | 0.844447 | 7.515994e-59 | -8.615850e-47 |
| Msa0212080 | Msa1129970 | 0.821536 | 3.832168e-53 | -8.615850e-47 |
| Msa0212080 | Msa1130010 | 0.856560 | 2.961680e-62 | -8.615850e-47 |
| Msa0212080 | Msa1145020 | 0.832638 | 8.435248e-56 | -8.615850e-47 |
| Msa0212080 | Msa1166400 | 0.815689 | 8.127065e-52 | -8.615850e-47 |
| Msa0212080 | Msa1169990 | 0.806962 | 6.375057e-50 | -8.615850e-47 |
| Msa0212080 | Msa1171310 | 0.835498 | 1.620359e-56 | -8.615850e-47 |
| Msa0212080 | Msa1201160 | 0.830384 | 3.027250e-55 | -8.615850e-47 |
| Msa0212080 | Msa1228190 | 0.809845 | 1.546764e-50 | -8.615850e-47 |
| Msa0212080 | Msa1294290 | 0.813877 | 2.049289e-51 | -8.615850e-47 |
| Msa0212080 | Msa1305510 | 0.829778 | 4.254955e-55 | -8.615850e-47 |
| Msa0212080 | Msa1378870 | 0.824647 | 7.207169e-54 | -8.615850e-47 |
| Msa0212080 | Msa1394890 | 0.815064 | 1.119320e-51 | -8.615850e-47 |
| Msa0212080 | Msa1406780 | 0.823465 | 1.364861e-53 | -8.615850e-47 |
| Msa0212080 | Msa1420060 | 0.826637 | 2.432398e-54 | -8.615850e-47 |
| Msa0212080 | Msa1421550 | 0.844625 | 6.732714e-59 | -8.615850e-47 |
| Msa0212080 | Msa1431850 | 0.829727 | 4.379427e-55 | -8.615850e-47 |
| Msa0212080 | Msa1459530 | 0.802265 | 6.089993e-49 | -8.615850e-47 |
| Msa0212080 | Msa1461060 | 0.825371 | 4.862158e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212080 | MtrunA17_Chr2g0311361 | 94.118 | 68 | 4 | 0 | 49 | 116 | 31 | 98 | 1.46e-44 | 140 |
| Msa0212080 | MtrunA17_Chr2g0324101 | 59.615 | 52 | 19 | 1 | 65 | 114 | 4 | 55 | 1.53e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212080 | AT1G34245.1 | 47.222 | 108 | 51 | 2 | 13 | 115 | 12 | 118 | 5.95e-29 | 101 |
| Msa0212080 | AT1G71866.2 | 68.627 | 51 | 15 | 1 | 65 | 115 | 85 | 134 | 1.11e-21 | 84.0 |
| Msa0212080 | AT1G71866.1 | 68.627 | 51 | 15 | 1 | 65 | 115 | 121 | 170 | 4.30e-21 | 83.2 |
| Msa0212080 | AT2G20875.1 | 59.615 | 52 | 19 | 1 | 65 | 114 | 53 | 104 | 1.82e-14 | 64.3 |
Find 33 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACAATTATAGACAAAA+AGG | 0.294093 | 2_1:-53821670 | Msa0212080:intron |
| ACATGTCACGTACCATGATT+TGG | 0.296793 | 2_1:+53821852 | None:intergenic |
| ACCTACCATGACCCTCTTGC+AGG | 0.342195 | 2_1:+53821457 | None:intergenic |
| AGGTTTCAGCAAATGTTCTA+TGG | 0.374663 | 2_1:-53821438 | Msa0212080:CDS |
| CATGATTTGGGATCACTCTA+AGG | 0.418584 | 2_1:+53821865 | None:intergenic |
| CATGTCACGTACCATGATTT+GGG | 0.459472 | 2_1:+53821853 | None:intergenic |
| AACAACTAGGAAAGATTTGT+GGG | 0.460826 | 2_1:+53821920 | None:intergenic |
| TAACAACTAGGAAAGATTTG+TGG | 0.480408 | 2_1:+53821919 | None:intergenic |
| TATGGCAGAGTCTTGCCCTA+TGG | 0.485018 | 2_1:-53821420 | Msa0212080:CDS |
| ACAATCTGGTATAGTGGAAC+CGG | 0.487458 | 2_1:+53821505 | None:intergenic |
| TTGTAGGAAGGAGCAAGAGA+AGG | 0.490101 | 2_1:-53821554 | Msa0212080:intron |
| GTTTACAGATGCATTTGTAA+AGG | 0.496002 | 2_1:-53821398 | Msa0212080:CDS |
| CCACATGCATGGGAACAATC+TGG | 0.502954 | 2_1:+53821491 | None:intergenic |
| CAAATGCATCTGTAAACCAT+AGG | 0.504305 | 2_1:+53821404 | None:intergenic |
| GTGAACATGGTCCACATGCA+TGG | 0.516739 | 2_1:+53821480 | None:intergenic |
| GTAATGGAACTCTATCCAAC+CGG | 0.520621 | 2_1:-53821524 | Msa0212080:CDS |
| ACCCTCTTGCAGGGTGAACA+TGG | 0.527410 | 2_1:+53821467 | None:intergenic |
| TCGATCAATTCGAGGGAACA+TGG | 0.534941 | 2_1:+53821366 | None:intergenic |
| AAGAGAAGGTAGCAATGTAA+TGG | 0.538637 | 2_1:-53821540 | Msa0212080:CDS |
| ATGGGAACAATCTGGTATAG+TGG | 0.545689 | 2_1:+53821499 | None:intergenic |
| TGAACATGGTCCACATGCAT+GGG | 0.559386 | 2_1:+53821481 | None:intergenic |
| AAATGCATCTGTAAACCATA+GGG | 0.566896 | 2_1:+53821405 | None:intergenic |
| CTTAGAGTGATCCCAAATCA+TGG | 0.569820 | 2_1:-53821864 | Msa0212080:intron |
| CCGTGAAGAAAATAACAACT+AGG | 0.577940 | 2_1:+53821907 | None:intergenic |
| ATCATCGATCGATCAATTCG+AGG | 0.580959 | 2_1:+53821358 | None:intergenic |
| GACCATGTTCACCCTGCAAG+AGG | 0.594033 | 2_1:-53821469 | Msa0212080:CDS |
| TTCACCCTGCAAGAGGGTCA+TGG | 0.604879 | 2_1:-53821462 | Msa0212080:CDS |
| CCCTGCAAGAGGGTCATGGT+AGG | 0.608435 | 2_1:-53821458 | Msa0212080:CDS |
| TCATCGATCGATCAATTCGA+GGG | 0.611769 | 2_1:+53821359 | None:intergenic |
| CCTACCATGACCCTCTTGCA+GGG | 0.620825 | 2_1:+53821458 | None:intergenic |
| CCAGATTGTTCCCATGCATG+TGG | 0.632279 | 2_1:-53821491 | Msa0212080:CDS |
| TCTGGTATAGTGGAACCGGT+TGG | 0.657904 | 2_1:+53821509 | None:intergenic |
| ACCATGTTCACCCTGCAAGA+GGG | 0.681360 | 2_1:-53821468 | Msa0212080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAACAATTATAGACAAAA+AGG | - | chr2_1:53821642-53821661 | Msa0212080:intron | 20.0% |
| ! | ACATCATTACATGATACTAT+GGG | + | chr2_1:53821537-53821556 | None:intergenic | 25.0% |
| ! | TACATCATTACATGATACTA+TGG | + | chr2_1:53821538-53821557 | None:intergenic | 25.0% |
| !!! | TTCCATATTTGTGTTTTTGT+AGG | - | chr2_1:53821742-53821761 | Msa0212080:intron | 25.0% |
| ! | TTCCTACAAAAACACAAATA+TGG | + | chr2_1:53821747-53821766 | None:intergenic | 25.0% |
| !!! | ATATTTGTGTTTTTGTAGGA+AGG | - | chr2_1:53821746-53821765 | Msa0212080:intron | 25.0% |
| AACAACTAGGAAAGATTTGT+GGG | + | chr2_1:53821395-53821414 | None:intergenic | 30.0% | |
| TAACAACTAGGAAAGATTTG+TGG | + | chr2_1:53821396-53821415 | None:intergenic | 30.0% | |
| !!! | CCTAGTTGTTATTTTCTTCA+CGG | - | chr2_1:53821405-53821424 | Msa0212080:CDS | 30.0% |
| ! | TTCTTCACGGTATTTTCTAT+TGG | - | chr2_1:53821418-53821437 | Msa0212080:CDS | 30.0% |
| AAACGAATGTGAAATACTCA+AGG | + | chr2_1:53821577-53821596 | None:intergenic | 30.0% | |
| AAGAAGTGAATGATCAATTG+TGG | - | chr2_1:53821718-53821737 | Msa0212080:intron | 30.0% | |
| AAATGCATCTGTAAACCATA+GGG | + | chr2_1:53821910-53821929 | None:intergenic | 30.0% | |
| GTTTACAGATGCATTTGTAA+AGG | - | chr2_1:53821914-53821933 | Msa0212080:CDS | 30.0% | |
| CCGTGAAGAAAATAACAACT+AGG | + | chr2_1:53821408-53821427 | None:intergenic | 35.0% | |
| ! | TCACGGTATTTTCTATTGGT+TGG | - | chr2_1:53821422-53821441 | Msa0212080:CDS | 35.0% |
| TGTAAGTGCATCAACTTATC+TGG | + | chr2_1:53821613-53821632 | None:intergenic | 35.0% | |
| AAGAGAAGGTAGCAATGTAA+TGG | - | chr2_1:53821772-53821791 | Msa0212080:intron | 35.0% | |
| AGGTTTCAGCAAATGTTCTA+TGG | - | chr2_1:53821874-53821893 | Msa0212080:CDS | 35.0% | |
| CAAATGCATCTGTAAACCAT+AGG | + | chr2_1:53821911-53821930 | None:intergenic | 35.0% | |
| ! | CATGATTTGGGATCACTCTA+AGG | + | chr2_1:53821450-53821469 | None:intergenic | 40.0% |
| CTTAGAGTGATCCCAAATCA+TGG | - | chr2_1:53821448-53821467 | Msa0212080:CDS | 40.0% | |
| CATGTCACGTACCATGATTT+GGG | + | chr2_1:53821462-53821481 | None:intergenic | 40.0% | |
| ACATGTCACGTACCATGATT+TGG | + | chr2_1:53821463-53821482 | None:intergenic | 40.0% | |
| ! | GTAATGGAACTCTATCCAAC+CGG | - | chr2_1:53821788-53821807 | Msa0212080:intron | 40.0% |
| ! | ACAATCTGGTATAGTGGAAC+CGG | + | chr2_1:53821810-53821829 | None:intergenic | 40.0% |
| ATGGGAACAATCTGGTATAG+TGG | + | chr2_1:53821816-53821835 | None:intergenic | 40.0% | |
| TTGTAGGAAGGAGCAAGAGA+AGG | - | chr2_1:53821758-53821777 | Msa0212080:intron | 45.0% | |
| TGAACATGGTCCACATGCAT+GGG | + | chr2_1:53821834-53821853 | None:intergenic | 45.0% | |
| ! | TCTGGTATAGTGGAACCGGT+TGG | + | chr2_1:53821806-53821825 | None:intergenic | 50.0% |
| CCACATGCATGGGAACAATC+TGG | + | chr2_1:53821824-53821843 | None:intergenic | 50.0% | |
| CCAGATTGTTCCCATGCATG+TGG | - | chr2_1:53821821-53821840 | Msa0212080:intron | 50.0% | |
| GTGAACATGGTCCACATGCA+TGG | + | chr2_1:53821835-53821854 | None:intergenic | 50.0% | |
| ACCATGTTCACCCTGCAAGA+GGG | - | chr2_1:53821844-53821863 | Msa0212080:intron | 50.0% | |
| TATGGCAGAGTCTTGCCCTA+TGG | - | chr2_1:53821892-53821911 | Msa0212080:CDS | 50.0% | |
| GACCATGTTCACCCTGCAAG+AGG | - | chr2_1:53821843-53821862 | Msa0212080:intron | 55.0% | |
| ACCCTCTTGCAGGGTGAACA+TGG | + | chr2_1:53821848-53821867 | None:intergenic | 55.0% | |
| TTCACCCTGCAAGAGGGTCA+TGG | - | chr2_1:53821850-53821869 | Msa0212080:intron | 55.0% | |
| CCTACCATGACCCTCTTGCA+GGG | + | chr2_1:53821857-53821876 | None:intergenic | 55.0% | |
| ACCTACCATGACCCTCTTGC+AGG | + | chr2_1:53821858-53821877 | None:intergenic | 55.0% | |
| CCCTGCAAGAGGGTCATGGT+AGG | - | chr2_1:53821854-53821873 | Msa0212080:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 53821370 | 53821964 | 53821370 | ID=Msa0212080;Name=Msa0212080 |
| chr2_1 | mRNA | 53821370 | 53821964 | 53821370 | ID=Msa0212080-mRNA-1;Parent=Msa0212080;Name=Msa0212080-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|3|0|116 |
| chr2_1 | exon | 53821865 | 53821964 | 53821865 | ID=Msa0212080-mRNA-1:exon:14955;Parent=Msa0212080-mRNA-1 |
| chr2_1 | exon | 53821671 | 53821720 | 53821671 | ID=Msa0212080-mRNA-1:exon:14954;Parent=Msa0212080-mRNA-1 |
| chr2_1 | exon | 53821370 | 53821570 | 53821370 | ID=Msa0212080-mRNA-1:exon:14953;Parent=Msa0212080-mRNA-1 |
| chr2_1 | CDS | 53821865 | 53821964 | 53821865 | ID=Msa0212080-mRNA-1:cds;Parent=Msa0212080-mRNA-1 |
| chr2_1 | CDS | 53821671 | 53821720 | 53821671 | ID=Msa0212080-mRNA-1:cds;Parent=Msa0212080-mRNA-1 |
| chr2_1 | CDS | 53821370 | 53821570 | 53821370 | ID=Msa0212080-mRNA-1:cds;Parent=Msa0212080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
