AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0252630


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252630 MtrunA17_Chr2g0311361 94.118 68 4 0 49 116 31 98 1.46e-44 140
Msa0252630 MtrunA17_Chr2g0324101 59.615 52 19 1 65 114 4 55 1.53e-14 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252630 AT1G34245.1 47.222 108 51 2 13 115 12 118 5.95e-29 101
Msa0252630 AT1G71866.2 68.627 51 15 1 65 115 85 134 1.11e-21 84.0
Msa0252630 AT1G71866.1 68.627 51 15 1 65 115 121 170 4.30e-21 83.2
Msa0252630 AT2G20875.1 59.615 52 19 1 65 114 53 104 1.82e-14 64.3

Find 33 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACAATTATAGACAAAA+AGG 0.294093 2_2:+44026259 Msa0252630:CDS
ACATGTCACGTACCATGATT+TGG 0.296793 2_2:-44026077 None:intergenic
ACCTACCATGACCCTCTTGC+AGG 0.342195 2_2:-44026472 None:intergenic
AGGTTTCAGCAAATGTTCTA+TGG 0.374663 2_2:+44026491 Msa0252630:CDS
CATGATTTGGGATCACTCTA+AGG 0.418584 2_2:-44026064 None:intergenic
CATGTCACGTACCATGATTT+GGG 0.459472 2_2:-44026076 None:intergenic
AACAACTAGGAAAGATTTGT+GGG 0.460826 2_2:-44026009 None:intergenic
TAACAACTAGGAAAGATTTG+TGG 0.480408 2_2:-44026010 None:intergenic
TATGGCAGAGTCTTGCCCTA+TGG 0.485018 2_2:+44026509 Msa0252630:CDS
ACAATCTGGTATAGTGGAAC+CGG 0.487458 2_2:-44026424 None:intergenic
TTGTAGGAAGGAGCAAGAGA+AGG 0.490101 2_2:+44026375 Msa0252630:intron
GTTTACAGATGCATTTGTAA+AGG 0.496002 2_2:+44026531 Msa0252630:CDS
CCACATGCATGGGAACAATC+TGG 0.502954 2_2:-44026438 None:intergenic
CAAATGCATCTGTAAACCAT+AGG 0.504305 2_2:-44026525 None:intergenic
GTGAACATGGTCCACATGCA+TGG 0.516739 2_2:-44026449 None:intergenic
GTAATGGAACTCTATCCAAC+CGG 0.520621 2_2:+44026405 Msa0252630:CDS
ACCCTCTTGCAGGGTGAACA+TGG 0.527410 2_2:-44026462 None:intergenic
TCGATCAATTCGAGGGAACA+TGG 0.534941 2_2:-44026563 None:intergenic
AAGAGAAGGTAGCAATGTAA+TGG 0.538637 2_2:+44026389 Msa0252630:CDS
ATGGGAACAATCTGGTATAG+TGG 0.545689 2_2:-44026430 None:intergenic
TGAACATGGTCCACATGCAT+GGG 0.559386 2_2:-44026448 None:intergenic
AAATGCATCTGTAAACCATA+GGG 0.566896 2_2:-44026524 None:intergenic
CTTAGAGTGATCCCAAATCA+TGG 0.569820 2_2:+44026065 Msa0252630:CDS
CCGTGAAGAAAATAACAACT+AGG 0.577940 2_2:-44026022 None:intergenic
ATCATCGATCGATCAATTCG+AGG 0.580959 2_2:-44026571 None:intergenic
GACCATGTTCACCCTGCAAG+AGG 0.594033 2_2:+44026460 Msa0252630:CDS
TTCACCCTGCAAGAGGGTCA+TGG 0.604879 2_2:+44026467 Msa0252630:CDS
CCCTGCAAGAGGGTCATGGT+AGG 0.608435 2_2:+44026471 Msa0252630:CDS
TCATCGATCGATCAATTCGA+GGG 0.611769 2_2:-44026570 None:intergenic
CCTACCATGACCCTCTTGCA+GGG 0.620825 2_2:-44026471 None:intergenic
CCAGATTGTTCCCATGCATG+TGG 0.632279 2_2:+44026438 Msa0252630:CDS
TCTGGTATAGTGGAACCGGT+TGG 0.657904 2_2:-44026420 None:intergenic
ACCATGTTCACCCTGCAAGA+GGG 0.681360 2_2:+44026461 Msa0252630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAACAATTATAGACAAAA+AGG + chr2_2:44026259-44026278 Msa0252630:CDS 20.0%
! ACATCATTACATGATACTAT+GGG - chr2_2:44026154-44026173 None:intergenic 25.0%
! TACATCATTACATGATACTA+TGG - chr2_2:44026155-44026174 None:intergenic 25.0%
!!! TTCCATATTTGTGTTTTTGT+AGG + chr2_2:44026359-44026378 Msa0252630:intron 25.0%
! TTCCTACAAAAACACAAATA+TGG - chr2_2:44026364-44026383 None:intergenic 25.0%
!!! ATATTTGTGTTTTTGTAGGA+AGG + chr2_2:44026363-44026382 Msa0252630:intron 25.0%
AACAACTAGGAAAGATTTGT+GGG - chr2_2:44026012-44026031 None:intergenic 30.0%
TAACAACTAGGAAAGATTTG+TGG - chr2_2:44026013-44026032 None:intergenic 30.0%
!!! CCTAGTTGTTATTTTCTTCA+CGG + chr2_2:44026022-44026041 Msa0252630:CDS 30.0%
! TTCTTCACGGTATTTTCTAT+TGG + chr2_2:44026035-44026054 Msa0252630:CDS 30.0%
AAACGAATGTGAAATACTCA+AGG - chr2_2:44026194-44026213 None:intergenic 30.0%
AAGAAGTGAATGATCAATTG+TGG + chr2_2:44026335-44026354 Msa0252630:intron 30.0%
AAATGCATCTGTAAACCATA+GGG - chr2_2:44026527-44026546 None:intergenic 30.0%
GTTTACAGATGCATTTGTAA+AGG + chr2_2:44026531-44026550 Msa0252630:CDS 30.0%
CCGTGAAGAAAATAACAACT+AGG - chr2_2:44026025-44026044 None:intergenic 35.0%
! TCACGGTATTTTCTATTGGT+TGG + chr2_2:44026039-44026058 Msa0252630:CDS 35.0%
TGTAAGTGCATCAACTTATC+TGG - chr2_2:44026230-44026249 None:intergenic 35.0%
AAGAGAAGGTAGCAATGTAA+TGG + chr2_2:44026389-44026408 Msa0252630:CDS 35.0%
AGGTTTCAGCAAATGTTCTA+TGG + chr2_2:44026491-44026510 Msa0252630:CDS 35.0%
CAAATGCATCTGTAAACCAT+AGG - chr2_2:44026528-44026547 None:intergenic 35.0%
! CATGATTTGGGATCACTCTA+AGG - chr2_2:44026067-44026086 None:intergenic 40.0%
CTTAGAGTGATCCCAAATCA+TGG + chr2_2:44026065-44026084 Msa0252630:CDS 40.0%
CATGTCACGTACCATGATTT+GGG - chr2_2:44026079-44026098 None:intergenic 40.0%
ACATGTCACGTACCATGATT+TGG - chr2_2:44026080-44026099 None:intergenic 40.0%
! GTAATGGAACTCTATCCAAC+CGG + chr2_2:44026405-44026424 Msa0252630:CDS 40.0%
! ACAATCTGGTATAGTGGAAC+CGG - chr2_2:44026427-44026446 None:intergenic 40.0%
ATGGGAACAATCTGGTATAG+TGG - chr2_2:44026433-44026452 None:intergenic 40.0%
TTGTAGGAAGGAGCAAGAGA+AGG + chr2_2:44026375-44026394 Msa0252630:intron 45.0%
TGAACATGGTCCACATGCAT+GGG - chr2_2:44026451-44026470 None:intergenic 45.0%
! TCTGGTATAGTGGAACCGGT+TGG - chr2_2:44026423-44026442 None:intergenic 50.0%
CCACATGCATGGGAACAATC+TGG - chr2_2:44026441-44026460 None:intergenic 50.0%
CCAGATTGTTCCCATGCATG+TGG + chr2_2:44026438-44026457 Msa0252630:CDS 50.0%
GTGAACATGGTCCACATGCA+TGG - chr2_2:44026452-44026471 None:intergenic 50.0%
ACCATGTTCACCCTGCAAGA+GGG + chr2_2:44026461-44026480 Msa0252630:CDS 50.0%
TATGGCAGAGTCTTGCCCTA+TGG + chr2_2:44026509-44026528 Msa0252630:CDS 50.0%
GACCATGTTCACCCTGCAAG+AGG + chr2_2:44026460-44026479 Msa0252630:CDS 55.0%
ACCCTCTTGCAGGGTGAACA+TGG - chr2_2:44026465-44026484 None:intergenic 55.0%
TTCACCCTGCAAGAGGGTCA+TGG + chr2_2:44026467-44026486 Msa0252630:CDS 55.0%
CCTACCATGACCCTCTTGCA+GGG - chr2_2:44026474-44026493 None:intergenic 55.0%
ACCTACCATGACCCTCTTGC+AGG - chr2_2:44026475-44026494 None:intergenic 55.0%
CCCTGCAAGAGGGTCATGGT+AGG + chr2_2:44026471-44026490 Msa0252630:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 44025987 44026581 44025987 ID=Msa0252630;Name=Msa0252630
chr2_2 mRNA 44025987 44026581 44025987 ID=Msa0252630-mRNA-1;Parent=Msa0252630;Name=Msa0252630-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|3|0|116
chr2_2 exon 44025987 44026086 44025987 ID=Msa0252630-mRNA-1:exon:13560;Parent=Msa0252630-mRNA-1
chr2_2 exon 44026231 44026280 44026231 ID=Msa0252630-mRNA-1:exon:13561;Parent=Msa0252630-mRNA-1
chr2_2 exon 44026381 44026581 44026381 ID=Msa0252630-mRNA-1:exon:13562;Parent=Msa0252630-mRNA-1
chr2_2 CDS 44025987 44026086 44025987 ID=Msa0252630-mRNA-1:cds;Parent=Msa0252630-mRNA-1
chr2_2 CDS 44026231 44026280 44026231 ID=Msa0252630-mRNA-1:cds;Parent=Msa0252630-mRNA-1
chr2_2 CDS 44026381 44026581 44026381 ID=Msa0252630-mRNA-1:cds;Parent=Msa0252630-mRNA-1
Gene Sequence

>Msa0252630

ATGAGGACTTACTCTCTTAAAGCCCACAAATCTTTCCTAGTTGTTATTTTCTTCACGGTATTTTCTATTGGTTGGTGCCTTAGAGTGATCCCAAATCATGATAAGTTGATGCACTTACAAGAAGAGAAAACAACAATTATAGACAAAAAGGAAGGAGCAAGAGAAGGTAGCAATGTAATGGAACTCTATCCAACCGGTTCCACTATACCAGATTGTTCCCATGCATGTGGACCATGTTCACCCTGCAAGAGGGTCATGGTAGGTTTCAGCAAATGTTCTATGGCAGAGTCTTGCCCTATGGTTTACAGATGCATTTGTAAAGGCAAATACTACCATGTTCCCTCGAATTGA

Protein sequence

>Msa0252630

MRTYSLKAHKSFLVVIFFTVFSIGWCLRVIPNHDKLMHLQEEKTTIIDKKEGAREGSNVMELYPTGSTIPDCSHACGPCSPCKRVMVGFSKCSMAESCPMVYRCICKGKYYHVPSN*