AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0239560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239560 MtrunA17_Chr2g0290461 98.684 76 1 0 1 76 1 76 2.65e-47 151
Msa0239560 MtrunA17_Chr4g0076261 80.556 72 14 0 4 75 8 79 3.64e-33 115
Msa0239560 MtrunA17_Chr7g0236071 73.333 75 20 0 1 75 1 75 1.08e-24 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239560 AT4G16380.1 74.324 74 19 0 3 76 7 80 9.83e-33 114
Msa0239560 AT4G16380.3 72.603 73 20 0 4 76 15 87 1.11e-31 112
Msa0239560 AT1G51090.1 60.526 76 30 0 1 76 1 76 2.71e-27 98.2
Msa0239560 AT1G49420.1 58.974 78 31 1 3 79 7 84 1.26e-24 92.0
Msa0239560 AT4G16380.2 73.333 45 12 0 32 76 15 59 2.84e-16 71.2

Find 30 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGAAATTCAAACCTTAT+AGG 0.213673 2_2:-14029005 Msa0239560:five_prime_UTR
TTGCTTTGTCATCATACTTT+TGG 0.238102 2_2:+14028320 None:intergenic
GACTCAGCTGGTTTGGGCTT+TGG 0.238535 2_2:+14028193 None:intergenic
TTCTCGGACTCAGCTGGTTT+GGG 0.239304 2_2:+14028187 None:intergenic
CTTCTCGGACTCAGCTGGTT+TGG 0.246232 2_2:+14028186 None:intergenic
CAAGAAATTCAAACCTTATA+GGG 0.285885 2_2:-14029004 Msa0239560:five_prime_UTR
AGCAGAGAAACAACACAAAA+TGG 0.336414 2_2:+14028921 None:intergenic
GTTGTAGTCCTGAGAAGATT+AGG 0.368435 2_2:-14028273 Msa0239560:CDS
AGCTGAGTCCGAGAAGAAAA+AGG 0.368826 2_2:-14028179 Msa0239560:CDS
ATAAACATGTAGGCCCTATA+AGG 0.393156 2_2:+14028991 None:intergenic
TTGTAGTCCTGAGAAGATTA+GGG 0.426247 2_2:-14028272 Msa0239560:CDS
AGAAATGCTACAAGAAGGTT+AGG 0.427970 2_2:-14028564 Msa0239560:CDS
TCAGCTGGTTTGGGCTTTGG+CGG 0.463353 2_2:+14028196 None:intergenic
TATCAAGAGTATTGAAATAG+TGG 0.464638 2_2:-14028221 Msa0239560:CDS
ATCGACAACCATGGCTGAAA+AGG 0.467264 2_2:-14028844 Msa0239560:intron
TTATTCAGGTGACCATTATG+AGG 0.467676 2_2:-14028609 Msa0239560:intron
AGCTTGTCCCTAATCTTCTC+AGG 0.499784 2_2:+14028265 None:intergenic
ATCAACCTTGAGCCTCATAA+TGG 0.505835 2_2:+14028597 None:intergenic
AGGGACAAGCTTTGTTACAA+AGG 0.533858 2_2:-14028253 Msa0239560:CDS
AAGCTTTGTTACAAAGGTGG+AGG 0.555529 2_2:-14028247 Msa0239560:CDS
GACAAGCTTTGTTACAAAGG+TGG 0.583958 2_2:-14028250 Msa0239560:CDS
GGTGACCATTATGAGGCTCA+AGG 0.605189 2_2:-14028602 Msa0239560:CDS
GTGTGAGAAATGCTACAAGA+AGG 0.610703 2_2:-14028569 Msa0239560:CDS
AGATTGTTGATAGACAGTGT+TGG 0.619844 2_2:+14029030 None:intergenic
AGCTTAATACATAAACATGT+AGG 0.630199 2_2:+14028981 None:intergenic
AGCAAACATTGTGACAATCA+CGG 0.650689 2_2:-14028302 Msa0239560:CDS
AGATCTCTGATCGACAACCA+TGG 0.651264 2_2:-14028853 Msa0239560:exon
TAGGCGTGTAAATCAAAAGA+CGG 0.656958 2_2:-14029175 None:intergenic
TCGATCAGAGATCTGATCTG+AGG 0.666948 2_2:+14028862 None:intergenic
AAACATTGTGACAATCACGG+TGG 0.804631 2_2:-14028299 Msa0239560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAACAATTAAAATTATACAT+AGG + chr2_2:14028689-14028708 None:intergenic 15.0%
!!! TTAATTGTTGATTTTTATTC+AGG - chr2_2:14028699-14028718 Msa0239560:intron 15.0%
!!! TATTGTTTTATTAGAATTTG+TGG - chr2_2:14028861-14028880 Msa0239560:five_prime_UTR 15.0%
!! ATTTACCAAAAAAATGTAAA+AGG - chr2_2:14028917-14028936 Msa0239560:five_prime_UTR 15.0%
!!! TATATCCTTTTACATTTTTT+TGG + chr2_2:14028925-14028944 None:intergenic 15.0%
!!! TATAAAATTGAGCTAATTTT+TGG - chr2_2:14028941-14028960 Msa0239560:five_prime_UTR 15.0%
!!! ATAAAATTGAGCTAATTTTT+GGG - chr2_2:14028942-14028961 Msa0239560:five_prime_UTR 15.0%
!! CAACAATTAAAATTATACAT+AGG + chr2_2:14028689-14028708 None:intergenic 15.0%
!!! TTAATTGTTGATTTTTATTC+AGG - chr2_2:14028699-14028718 Msa0239560:intron 15.0%
!!! TATTGTTTTATTAGAATTTG+TGG - chr2_2:14028861-14028880 Msa0239560:five_prime_UTR 15.0%
!! ATTTACCAAAAAAATGTAAA+AGG - chr2_2:14028917-14028936 Msa0239560:five_prime_UTR 15.0%
!!! TATATCCTTTTACATTTTTT+TGG + chr2_2:14028925-14028944 None:intergenic 15.0%
!!! TATAAAATTGAGCTAATTTT+TGG - chr2_2:14028941-14028960 Msa0239560:five_prime_UTR 15.0%
!!! ATAAAATTGAGCTAATTTTT+GGG - chr2_2:14028942-14028961 Msa0239560:five_prime_UTR 15.0%
!! AATAAAAGAATGTCTTCATA+TGG + chr2_2:14028639-14028658 None:intergenic 20.0%
!! AATAAAAGAATGTCTTCATA+TGG + chr2_2:14028639-14028658 None:intergenic 20.0%
! ACAAGAAATTCAAACCTTAT+AGG - chr2_2:14028317-14028336 Msa0239560:CDS 25.0%
! CAAGAAATTCAAACCTTATA+GGG - chr2_2:14028318-14028337 Msa0239560:CDS 25.0%
! AGCTTAATACATAAACATGT+AGG + chr2_2:14028344-14028363 None:intergenic 25.0%
!! ATCATAAATTTGTTTCACCA+TGG - chr2_2:14028662-14028681 Msa0239560:intron 25.0%
! TAAAATTATACATAGGACCA+TGG + chr2_2:14028682-14028701 None:intergenic 25.0%
! ATAGTTAGAAATGAAAGTAG+AGG + chr2_2:14028818-14028837 None:intergenic 25.0%
! TAAAAACACTTGTATGCATT+TGG - chr2_2:14028894-14028913 Msa0239560:five_prime_UTR 25.0%
!!! TATCAAGAGTATTGAAATAG+TGG - chr2_2:14029101-14029120 Msa0239560:five_prime_UTR 25.0%
! ACAAGAAATTCAAACCTTAT+AGG - chr2_2:14028317-14028336 Msa0239560:CDS 25.0%
! CAAGAAATTCAAACCTTATA+GGG - chr2_2:14028318-14028337 Msa0239560:CDS 25.0%
! AGCTTAATACATAAACATGT+AGG + chr2_2:14028344-14028363 None:intergenic 25.0%
!! ATCATAAATTTGTTTCACCA+TGG - chr2_2:14028662-14028681 Msa0239560:intron 25.0%
! TAAAATTATACATAGGACCA+TGG + chr2_2:14028682-14028701 None:intergenic 25.0%
! ATAGTTAGAAATGAAAGTAG+AGG + chr2_2:14028818-14028837 None:intergenic 25.0%
! TAAAAACACTTGTATGCATT+TGG - chr2_2:14028894-14028913 Msa0239560:five_prime_UTR 25.0%
!!! TATCAAGAGTATTGAAATAG+TGG - chr2_2:14029101-14029120 Msa0239560:five_prime_UTR 25.0%
! TGACAATCATGTGTTTGTTT+TGG + chr2_2:14028550-14028569 None:intergenic 30.0%
! TTGCTTTGTCATCATACTTT+TGG + chr2_2:14029005-14029024 None:intergenic 30.0%
! TGACAATCATGTGTTTGTTT+TGG + chr2_2:14028550-14028569 None:intergenic 30.0%
! TTGCTTTGTCATCATACTTT+TGG + chr2_2:14029005-14029024 None:intergenic 30.0%
! GGTTTTTGCTAGAACTGAAA+GGG + chr2_2:14028274-14028293 None:intergenic 35.0%
! TGGTTTTTGCTAGAACTGAA+AGG + chr2_2:14028275-14028294 None:intergenic 35.0%
! AGATTGTTGATAGACAGTGT+TGG + chr2_2:14028295-14028314 None:intergenic 35.0%
ATAAACATGTAGGCCCTATA+AGG + chr2_2:14028334-14028353 None:intergenic 35.0%
AGCAGAGAAACAACACAAAA+TGG + chr2_2:14028404-14028423 None:intergenic 35.0%
TTATTCAGGTGACCATTATG+AGG - chr2_2:14028713-14028732 Msa0239560:intron 35.0%
AGAAATGCTACAAGAAGGTT+AGG - chr2_2:14028758-14028777 Msa0239560:intron 35.0%
AGCAAACATTGTGACAATCA+CGG - chr2_2:14029020-14029039 Msa0239560:five_prime_UTR 35.0%
TTGTAGTCCTGAGAAGATTA+GGG - chr2_2:14029050-14029069 Msa0239560:five_prime_UTR 35.0%
! GGTTTTTGCTAGAACTGAAA+GGG + chr2_2:14028274-14028293 None:intergenic 35.0%
! TGGTTTTTGCTAGAACTGAA+AGG + chr2_2:14028275-14028294 None:intergenic 35.0%
! AGATTGTTGATAGACAGTGT+TGG + chr2_2:14028295-14028314 None:intergenic 35.0%
ATAAACATGTAGGCCCTATA+AGG + chr2_2:14028334-14028353 None:intergenic 35.0%
AGCAGAGAAACAACACAAAA+TGG + chr2_2:14028404-14028423 None:intergenic 35.0%
TTATTCAGGTGACCATTATG+AGG - chr2_2:14028713-14028732 Msa0239560:intron 35.0%
AGAAATGCTACAAGAAGGTT+AGG - chr2_2:14028758-14028777 Msa0239560:intron 35.0%
AGCAAACATTGTGACAATCA+CGG - chr2_2:14029020-14029039 Msa0239560:five_prime_UTR 35.0%
TTGTAGTCCTGAGAAGATTA+GGG - chr2_2:14029050-14029069 Msa0239560:five_prime_UTR 35.0%
! ACACTTTACCTTTTCAGCCA+TGG + chr2_2:14028489-14028508 None:intergenic 40.0%
ATCAACCTTGAGCCTCATAA+TGG + chr2_2:14028728-14028747 None:intergenic 40.0%
GTGTGAGAAATGCTACAAGA+AGG - chr2_2:14028753-14028772 Msa0239560:intron 40.0%
AAACATTGTGACAATCACGG+TGG - chr2_2:14029023-14029042 Msa0239560:five_prime_UTR 40.0%
GTTGTAGTCCTGAGAAGATT+AGG - chr2_2:14029049-14029068 Msa0239560:five_prime_UTR 40.0%
!! AGGGACAAGCTTTGTTACAA+AGG - chr2_2:14029069-14029088 Msa0239560:five_prime_UTR 40.0%
!! GACAAGCTTTGTTACAAAGG+TGG - chr2_2:14029072-14029091 Msa0239560:five_prime_UTR 40.0%
!! AAGCTTTGTTACAAAGGTGG+AGG - chr2_2:14029075-14029094 Msa0239560:five_prime_UTR 40.0%
! ACACTTTACCTTTTCAGCCA+TGG + chr2_2:14028489-14028508 None:intergenic 40.0%
ATCAACCTTGAGCCTCATAA+TGG + chr2_2:14028728-14028747 None:intergenic 40.0%
GTGTGAGAAATGCTACAAGA+AGG - chr2_2:14028753-14028772 Msa0239560:intron 40.0%
AAACATTGTGACAATCACGG+TGG - chr2_2:14029023-14029042 Msa0239560:five_prime_UTR 40.0%
GTTGTAGTCCTGAGAAGATT+AGG - chr2_2:14029049-14029068 Msa0239560:five_prime_UTR 40.0%
!! AGGGACAAGCTTTGTTACAA+AGG - chr2_2:14029069-14029088 Msa0239560:five_prime_UTR 40.0%
!! GACAAGCTTTGTTACAAAGG+TGG - chr2_2:14029072-14029091 Msa0239560:five_prime_UTR 40.0%
!! AAGCTTTGTTACAAAGGTGG+AGG - chr2_2:14029075-14029094 Msa0239560:five_prime_UTR 40.0%
TCGATCAGAGATCTGATCTG+AGG + chr2_2:14028463-14028482 None:intergenic 45.0%
AGATCTCTGATCGACAACCA+TGG - chr2_2:14028469-14028488 Msa0239560:intron 45.0%
ATCGACAACCATGGCTGAAA+AGG - chr2_2:14028478-14028497 Msa0239560:intron 45.0%
TAGAGGTGATGAACTCACGA+GGG + chr2_2:14028801-14028820 None:intergenic 45.0%
AGCTTGTCCCTAATCTTCTC+AGG + chr2_2:14029060-14029079 None:intergenic 45.0%
! TTTTTCTTCTCGGACTCAGC+TGG + chr2_2:14029144-14029163 None:intergenic 45.0%
AGCTGAGTCCGAGAAGAAAA+AGG - chr2_2:14029143-14029162 Msa0239560:five_prime_UTR 45.0%
GAGTCCGAGAAGAAAAAGGA+AGG - chr2_2:14029147-14029166 Msa0239560:five_prime_UTR 45.0%
! CTCGCCTTCCTTTTTCTTCT+CGG + chr2_2:14029154-14029173 None:intergenic 45.0%
TCGATCAGAGATCTGATCTG+AGG + chr2_2:14028463-14028482 None:intergenic 45.0%
AGATCTCTGATCGACAACCA+TGG - chr2_2:14028469-14028488 Msa0239560:intron 45.0%
ATCGACAACCATGGCTGAAA+AGG - chr2_2:14028478-14028497 Msa0239560:intron 45.0%
TAGAGGTGATGAACTCACGA+GGG + chr2_2:14028801-14028820 None:intergenic 45.0%
AGCTTGTCCCTAATCTTCTC+AGG + chr2_2:14029060-14029079 None:intergenic 45.0%
! TTTTTCTTCTCGGACTCAGC+TGG + chr2_2:14029144-14029163 None:intergenic 45.0%
AGCTGAGTCCGAGAAGAAAA+AGG - chr2_2:14029143-14029162 Msa0239560:five_prime_UTR 45.0%
GAGTCCGAGAAGAAAAAGGA+AGG - chr2_2:14029147-14029166 Msa0239560:five_prime_UTR 45.0%
! CTCGCCTTCCTTTTTCTTCT+CGG + chr2_2:14029154-14029173 None:intergenic 45.0%
GGTGACCATTATGAGGCTCA+AGG - chr2_2:14028720-14028739 Msa0239560:intron 50.0%
GTAGAGGTGATGAACTCACG+AGG + chr2_2:14028802-14028821 None:intergenic 50.0%
! TTCTCGGACTCAGCTGGTTT+GGG + chr2_2:14029138-14029157 None:intergenic 50.0%
GGTGACCATTATGAGGCTCA+AGG - chr2_2:14028720-14028739 Msa0239560:intron 50.0%
GTAGAGGTGATGAACTCACG+AGG + chr2_2:14028802-14028821 None:intergenic 50.0%
! TTCTCGGACTCAGCTGGTTT+GGG + chr2_2:14029138-14029157 None:intergenic 50.0%
!! TCAGCTGGTTTGGGCTTTGG+CGG + chr2_2:14029129-14029148 None:intergenic 55.0%
!! GACTCAGCTGGTTTGGGCTT+TGG + chr2_2:14029132-14029151 None:intergenic 55.0%
CTTCTCGGACTCAGCTGGTT+TGG + chr2_2:14029139-14029158 None:intergenic 55.0%
!! TCAGCTGGTTTGGGCTTTGG+CGG + chr2_2:14029129-14029148 None:intergenic 55.0%
!! GACTCAGCTGGTTTGGGCTT+TGG + chr2_2:14029132-14029151 None:intergenic 55.0%
CTTCTCGGACTCAGCTGGTT+TGG + chr2_2:14029139-14029158 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 14028153 14029191 14028153 ID=Msa0239560;Name=Msa0239560
chr2_2 mRNA 14028153 14029191 14028153 ID=Msa0239560-mRNA-1;Parent=Msa0239560;Name=Msa0239560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=335|1|1|1|1|1|3|0|98
chr2_2 exon 14028153 14028352 14028153 ID=Msa0239560-mRNA-1:exon:5841;Parent=Msa0239560-mRNA-1
chr2_2 exon 14028542 14028623 14028542 ID=Msa0239560-mRNA-1:exon:5840;Parent=Msa0239560-mRNA-1
chr2_2 exon 14028845 14029191 14028845 ID=Msa0239560-mRNA-1:exon:5839;Parent=Msa0239560-mRNA-1
chr2_2 five_prime_UTR 14028857 14029191 14028857 ID=Msa0239560-mRNA-1:five_prime_utr;Parent=Msa0239560-mRNA-1
chr2_2 CDS 14028845 14028856 14028845 ID=Msa0239560-mRNA-1:cds;Parent=Msa0239560-mRNA-1
chr2_2 CDS 14028542 14028623 14028542 ID=Msa0239560-mRNA-1:cds;Parent=Msa0239560-mRNA-1
chr2_2 CDS 14028153 14028352 14028153 ID=Msa0239560-mRNA-1:cds;Parent=Msa0239560-mRNA-1
Gene Sequence

>Msa0239560

ATGGCTGAAAAGGTGACCATTATGAGGCTCAAGGTTGATCTTGAGTGTGAGAAATGCTACAAGAAGGTTAGGAAGCTTCTCAACAAGTACCCTCAAATTCGAGACCAAAAGTATGATGACAAAGCAAACATTGTGACAATCACGGTGGTATGTTGTAGTCCTGAGAAGATTAGGGACAAGCTTTGTTACAAAGGTGGAGGTTCTATCAAGAGTATTGAAATAGTGGATCCGCCAAAGCCCAAACCAGCTGAGTCCGAGAAGAAAAAGGAAGGCGAGAAGCCCAAGTCCGCTGAG

Protein sequence

>Msa0239560

MAEKVTIMRLKVDLECEKCYKKVRKLLNKYPQIRDQKYDDKANIVTITVVCCSPEKIRDKLCYKGGGSIKSIEIVDPPKPKPAESEKKKEGEKPKSAE