AlfalfaGEDB | Msa0216150

M. sativa cultivar zhongmu-4 / Msa0216150

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0004791 GO:0004857 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006950 GO:0006979 GO:0008047 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0015035 GO:0015036 GO:0016020 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019222 GO:0019725 GO:0019904 GO:0030234 GO:0030312 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0033554 GO:0034357 GO:0034599 GO:0042221 GO:0042592 GO:0042651 GO:0043085 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051716 GO:0055035 GO:0055114 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071944 GO:0080090 GO:0097237 GO:0098754 GO:0098772 GO:0098869 GO:1990748
PFAM: PF00085.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216150	XP_013464712.1	91.908	173	13	1	1	172	1	173	1.52e-109	320

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216150	sp\|Q9SEU6\|TRXM4_ARATH	60.510	157	58	2	20	172	35	191	4.86e-65	200

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216150	I3SA84	91.908	173	13	1	1	172	1	173	7.27e-110	320

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0216150	0.898318	5.968614e-77	-8.615850e-47
Msa0000800	Msa0216150	-0.844853	5.845789e-59	-8.615850e-47
Msa0001510	Msa0216150	0.836500	9.024673e-57	-8.615850e-47
Msa0002110	Msa0216150	0.908740	1.228633e-81	-8.615850e-47
Msa0002690	Msa0216150	0.866984	1.911568e-65	-8.615850e-47
Msa0002700	Msa0216150	0.865220	6.913240e-65	-8.615850e-47
Msa0003340	Msa0216150	0.878612	2.438730e-69	-8.615850e-47
Msa0004180	Msa0216150	0.881314	2.661444e-70	-8.615850e-47
Msa0005500	Msa0216150	0.834604	2.722270e-56	-8.615850e-47
Msa0006080	Msa0216150	-0.878748	2.184926e-69	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216150	MtrunA17_Chr2g0317731	91.908	173	13	1	1	172	1	173	1.40e-113	320
Msa0216150	MtrunA17_Chr8g0360031	64.835	182	48	6	1	172	1	176	2.62e-74	220
Msa0216150	MtrunA17_Chr3g0125821	65.600	125	42	1	48	172	50	173	3.73e-61	187
Msa0216150	MtrunA17_Chr4g0044731	53.125	96	45	0	77	172	73	168	2.93e-37	126
Msa0216150	MtrunA17_Chr2g0317721	48.039	102	53	0	69	170	41	142	6.31e-33	114
Msa0216150	MtrunA17_Chr2g0317701	52.941	102	47	1	69	169	46	147	5.15e-32	112
Msa0216150	MtrunA17_Chr2g0317711	48.077	104	53	1	69	172	41	143	7.28e-28	101
Msa0216150	MtrunA17_Chr1g0211871	38.532	109	59	2	69	172	70	175	4.58e-23	90.1
Msa0216150	MtrunA17_Chr1g0201781	33.981	103	68	0	67	169	60	162	3.20e-20	82.4
Msa0216150	MtrunA17_Chr2g0317691	51.562	64	31	0	109	172	3	66	5.98e-20	79.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216150	AT3G15360.1	60.510	157	58	2	20	172	35	191	4.95e-66	200
Msa0216150	AT1G03680.1	53.548	155	68	3	18	172	29	179	2.47e-55	172
Msa0216150	AT4G03520.1	52.632	152	66	4	24	172	37	185	1.19e-53	169
Msa0216150	AT2G15570.1	50.450	111	52	1	62	172	64	171	1.83e-37	127
Msa0216150	AT2G15570.2	49.550	111	54	1	62	172	64	172	3.00e-37	126
Msa0216150	AT4G03520.2	63.380	71	26	0	102	172	1	71	1.38e-29	103
Msa0216150	AT1G76760.1	36.792	106	67	0	67	172	63	168	5.21e-23	90.1
Msa0216150	AT1G43560.1	34.266	143	84	2	35	172	26	163	2.09e-22	88.6
Msa0216150	AT1G50320.1	33.577	137	76	2	38	172	53	176	4.51e-21	85.5
Msa0216150	AT1G19730.1	42.105	76	44	0	83	158	26	101	4.47e-18	75.9

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Find 69 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCCAGTGATGTCAAA+TGG	0.470133	2_1:-61859723	None:intergenic
AAAGTCCTTCAGTTGCTACT+CGG	0.607500	2_1:+61859358	Msa0216150:CDS
AACCGAACCCTAACCTTCAA+CGG	0.535342	2_1:-61856450	None:intergenic
AAGTCTCCGCCACTCGTCTG+CGG	0.566372	2_1:+61856360	Msa0216150:CDS
AATCTCAGATTCTATGACAA+GGG	0.589830	2_1:-61859214	None:intergenic
ACCGCGACGAACAACATTCG+AGG	0.568696	2_1:-61856393	None:intergenic
AGAATCTGAGATTCCTGTGT+TGG	0.562341	2_1:+61859224	Msa0216150:CDS
AGCTTGAGGCCGGTGTATTG+AGG	0.508457	2_1:-61856332	None:intergenic
ATCACGAGCCTCACACACGA+CGG	0.693040	2_1:-61856420	None:intergenic
ATGCGGCAGGGACCACACCA+TGG	0.592003	2_1:-61859261	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGATCTGTTGAAAGTGAT+TGG	+	chr2_1:61856510-61856529	Msa0216150:intron	30.0%
TAGGTGACTTACAAAACTAA+CGG	-	chr2_1:61856596-61856615	None:intergenic	30.0%
CTGAAATTTAGTGTACGTAT+TGG	+	chr2_1:61856650-61856669	Msa0216150:intron	30.0%
AAACATTGACATTAACTGAC+GGG	+	chr2_1:61856790-61856809	Msa0216150:intron	30.0%
TTTCATGTGTTTCGGATTAT+GGG	-	chr2_1:61856846-61856865	None:intergenic	30.0%
CTTCAATTGTAGACATTGAA+AGG	+	chr2_1:61856999-61857018	Msa0216150:intron	30.0%
AGTCTACATTGCACTAAAAA+GGG	+	chr2_1:61857090-61857109	Msa0216150:intron	30.0%
TATATATTAAAAGAGCCACG+TGG	+	chr2_1:61857309-61857328	Msa0216150:intron	30.0%
GGCCTAATATTCATAATGTT+AGG	+	chr2_1:61857592-61857611	Msa0216150:intron	30.0%
ATTGAACATCATCACACATA+AGG	+	chr2_1:61857650-61857669	Msa0216150:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	61856099	61859740	61856099	ID=Msa0216150;Name=Msa0216150
chr2_1	mRNA	61856099	61859740	61856099	ID=Msa0216150-mRNA-1;Parent=Msa0216150;Name=Msa0216150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=155\|1\|1\|1\|1\|1\|2\|243\|172
chr2_1	exon	61856099	61856458	61856099	ID=Msa0216150-mRNA-1:exon:17072;Parent=Msa0216150-mRNA-1
chr2_1	exon	61859184	61859740	61859184	ID=Msa0216150-mRNA-1:exon:17073;Parent=Msa0216150-mRNA-1
chr2_1	five_prime_UTR	61856099	61856253	61856099	ID=Msa0216150-mRNA-1:five_prime_utr;Parent=Msa0216150-mRNA-1
chr2_1	CDS	61856254	61856458	61856254	ID=Msa0216150-mRNA-1:cds;Parent=Msa0216150-mRNA-1
chr2_1	CDS	61859184	61859497	61859184	ID=Msa0216150-mRNA-1:cds;Parent=Msa0216150-mRNA-1
chr2_1	three_prime_UTR	61859498	61859740	61859498	ID=Msa0216150-mRNA-1:three_prime_utr;Parent=Msa0216150-mRNA-1

Gene Sequence

>Msa0216150

ATGGCTACCGTACAACTCGAATCCTTCTCCCTTATTCGCTCTCCAATCGTCGCTTCTCGCCTGAGCACTGCCGCGTTTCCTCAATACACCGGCCTCAAGCTCCGACAAGTCTCCGCCACTCGTCTGCGGAGTCAACCCGCCTCGAATGTTGTTCGTCGCGGTGGAACCGTCGTGTGTGAGGCTCGTGATAACACAGCCGTTGAAGTGGCTTCTATTACTGATGGAAATTGGCAATCCCTTGTCATAGAATCTGAGATTCCTGTGTTGGTCGAATTCTGGGCTCCATGGTGTGGTCCCTGCCGCATGATTCATCCCATAGTTGATGAGCTAGCAAAGGAATATGCGGGTAAGCTCAAGTGTTACAAACTCAATACTGATGAAAGTCCTTCAGTTGCTACTCGGTATGGGATTCGAAGTATTCCAACTGTCATTTTCTTCAAGAACGGTGAAAAGAAAGATACAGTTATTGGTGCTGTGCCCAAAGCAACATTGACCACAAATATAGAAAAATTCCTGTGA

Protein sequence

>Msa0216150

MATVQLESFSLIRSPIVASRLSTAAFPQYTGLKLRQVSATRLRSQPASNVVRRGGTVVCEARDNTAVEVASITDGNWQSLVIESEIPVLVEFWAPWCGPCRMIHPIVDELAKEYAGKLKCYKLNTDESPSVATRYGIRSIPTVIFFKNGEKKDTVIGAVPKATLTTNIEKFL*