AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0395770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0395770 MtrunA17_Chr3g0081641 97.802 91 2 0 1 91 1 91 1.60e-60 179
Msa0395770 MtrunA17_Chr1g0173721 75.000 92 12 3 1 91 1 82 2.51e-41 130
Msa0395770 MtrunA17_Chr5g0404111 39.394 66 38 2 1 64 14 79 5.20e-13 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0395770 AT4G39235.2 64.835 91 27 1 1 91 1 86 6.83e-36 117
Msa0395770 AT4G39235.1 64.835 91 27 1 1 91 1 86 6.83e-36 117
Msa0395770 AT3G05570.1 63.043 92 31 2 1 91 1 90 1.08e-34 114
Msa0395770 AT2G21640.1 50.538 93 42 3 1 89 10 102 4.94e-23 85.1

Find 53 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAAGTCCAACCCACTTTA+AGG 0.160372 3_2:-12863818 Msa0395770:five_prime_UTR
GTGACCAAGATAAGATAATT+TGG 0.179266 3_2:-12863915 Msa0395770:five_prime_UTR
CTTAAAGTGGGTTGGACTTT+CGG 0.190621 3_2:+12863819 None:intergenic
TTAAAGTGGGTTGGACTTTC+GGG 0.195510 3_2:+12863820 None:intergenic
CGAGTTTATTCATGCTTCTA+TGG 0.253989 3_2:-12860979 Msa0395770:CDS
TTGGATCTATTTCAGATGTT+TGG 0.301015 3_2:-12860840 Msa0395770:intron
TTTCCCAAATTATCTTATCT+TGG 0.355370 3_2:+12863911 None:intergenic
ATATTGTACGTATAATTATA+CGG 0.377573 3_2:-12863968 Msa0395770:five_prime_UTR
GAGAAGAGGGAATGAGAAAG+AGG 0.383559 3_2:-12864008 Msa0395770:five_prime_UTR
GCATGAAGAAGCCTCATTCT+TGG 0.389977 3_2:-12861024 Msa0395770:CDS
TGAGAGTTGTTTATGAAAGA+TGG 0.392410 3_2:+12863794 None:intergenic
CACGTGAGAGTAGCATATTT+TGG 0.392651 3_2:-12863876 Msa0395770:five_prime_UTR
TTCAACTTCTTTCGTAGCCT+TGG 0.399403 3_2:+12860788 None:intergenic
ATGAAAGATGGGCCTTAAAG+TGG 0.400636 3_2:+12863806 None:intergenic
TGACCAAGATAAGATAATTT+GGG 0.410922 3_2:-12863914 Msa0395770:five_prime_UTR
AGCTATGTTATTACTGTTTG+TGG 0.415073 3_2:+12860621 None:intergenic
TGTTTAACCGAGTCATGTAT+TGG 0.415747 3_2:-12860707 Msa0395770:three_prime_UTR
CTTCTTCTTTCGACAAGAAG+TGG 0.455215 3_2:+12861046 None:intergenic
GTTTGGTTTGTCCAAGGTAG+TGG 0.458713 3_2:-12860823 Msa0395770:CDS
CTTTCAGAAAACAATTAAGA+AGG 0.462199 3_2:-12860940 Msa0395770:intron
AGAAGAGGGAATGAGAAAGA+GGG 0.462578 3_2:-12864007 Msa0395770:five_prime_UTR
TAATTATACGGTTACGGTGT+GGG 0.466712 3_2:-12863956 Msa0395770:five_prime_UTR
TTTCCGGTGAGTTGAGTTGA+AGG 0.488754 3_2:+12863768 None:intergenic
ATATAAAAGTAGGTACGAGC+AGG 0.494703 3_2:+12860595 None:intergenic
GTATTAAGACAATGTATCTC+AGG 0.498600 3_2:+12860668 None:intergenic
GCTATTGGAACCTCAGATCA+TGG 0.502244 3_2:-12861117 Msa0395770:exon
TTCTGAGTGTCCATGATCTG+AGG 0.505531 3_2:+12861107 None:intergenic
TACGTATAATTATACGGTTA+CGG 0.508897 3_2:-12863962 Msa0395770:five_prime_UTR
TGTATAAAGATGTCTGTTTG+TGG 0.516291 3_2:+12860644 None:intergenic
GAGAGTTGTTTATGAAAGAT+GGG 0.516673 3_2:+12863795 None:intergenic
CTCATTCTTGGAGGATGTGA+AGG 0.535627 3_2:-12861012 Msa0395770:CDS
AAGAGAGACGGAAAAGTGAA+TGG 0.537211 3_2:+12864045 None:intergenic
CTTCACATCCTCCAAGAATG+AGG 0.544616 3_2:+12861013 None:intergenic
GCAACAGCAGCTGATGAAGT+TGG 0.545155 3_2:+12861068 None:intergenic
AACTCACCGGAAAACTCAGC+CGG 0.554579 3_2:-12863758 Msa0395770:five_prime_UTR
CATGACTCGGTTAAACATAG+TGG 0.555451 3_2:+12860713 None:intergenic
AGATGGGCCTTAAAGTGGGT+TGG 0.557497 3_2:+12863811 None:intergenic
GTTCGCTTCAGCCACTACCT+TGG 0.562123 3_2:+12860812 None:intergenic
TCAATTCGTCGCAAGAGAGA+CGG 0.564305 3_2:+12864033 None:intergenic
TGAAGAAGCCTCATTCTTGG+AGG 0.565258 3_2:-12861021 Msa0395770:CDS
TCAGATGTTTGGTTTGTCCA+AGG 0.567621 3_2:-12860829 Msa0395770:intron
CGACGAATTGACAGAGAAGA+GGG 0.570597 3_2:-12864021 Msa0395770:five_prime_UTR
TGAAAGATGGGCCTTAAAGT+GGG 0.577116 3_2:+12863807 None:intergenic
AAACAGCGAAAACTCCGTCC+AGG 0.606658 3_2:-12863715 Msa0395770:intron
TCGCCTTCAACTCAACTCAC+CGG 0.609709 3_2:-12863771 Msa0395770:five_prime_UTR
GCGACGAATTGACAGAGAAG+AGG 0.626167 3_2:-12864022 Msa0395770:five_prime_UTR
ATAATTATACGGTTACGGTG+TGG 0.631391 3_2:-12863957 Msa0395770:five_prime_UTR
TATAAAAGTAGGTACGAGCA+GGG 0.632335 3_2:+12860596 None:intergenic
TGGTGGCCGACGATGAACTC+CGG 0.639753 3_2:+12863739 None:intergenic
AGTGGCTGAAGCGAACTCCA+AGG 0.641621 3_2:-12860805 Msa0395770:CDS
AATGTGTCCAATACATGACT+CGG 0.646526 3_2:+12860700 None:intergenic
ACTCAGCCGGAGTTCATCGT+CGG 0.658290 3_2:-12863745 Msa0395770:five_prime_UTR
AATTTGGGAAACTGTGTCTG+TGG 0.705142 3_2:-12863899 Msa0395770:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATGTAGAAATATTA+TGG - chr3_2:12861091-12861110 Msa0395770:CDS 10.0%
!!! ATTAGAATTATTTTTTGAAT+TGG - chr3_2:12861118-12861137 Msa0395770:exon 10.0%
!! ATATTGTACGTATAATTATA+CGG - chr3_2:12860698-12860717 Msa0395770:three_prime_UTR 15.0%
!! TCTACATTAAATTATCAAAA+CGG + chr3_2:12861084-12861103 None:intergenic 15.0%
!!! AATTGAAAATTATCTTGTTT+AGG - chr3_2:12861190-12861209 Msa0395770:intron 15.0%
!!! ATTGAAAATTATCTTGTTTA+GGG - chr3_2:12861191-12861210 Msa0395770:intron 15.0%
!!! TTAAAACAGTTTAAAGTAAT+GGG + chr3_2:12861714-12861733 None:intergenic 15.0%
!!! TGAATTTTAAGTAATGTTTA+AGG - chr3_2:12861768-12861787 Msa0395770:intron 15.0%
!! TTCTTTATAAATAAACTATG+CGG + chr3_2:12862813-12862832 None:intergenic 15.0%
!!! ATTACTAAATTTTACAAGTT+TGG + chr3_2:12862945-12862964 None:intergenic 15.0%
!! TTAGAATATTTAAAAGCAAA+AGG + chr3_2:12863219-12863238 None:intergenic 15.0%
!!! GTTATTGCATTTTAATATTT+TGG - chr3_2:12863419-12863438 Msa0395770:intron 15.0%
!! AGTTAAACATTAGTATATAT+CGG + chr3_2:12863484-12863503 None:intergenic 15.0%
!!! ACAAATTTACTCTTTTAAAA+CGG + chr3_2:12863923-12863942 None:intergenic 15.0%
!!! GAATTTCAAATGATATGTTT+TGG - chr3_2:12861140-12861159 Msa0395770:intron 20.0%
!!! CTTAAAACAGTTTAAAGTAA+TGG + chr3_2:12861715-12861734 None:intergenic 20.0%
!!! CATTACTTTAAACTGTTTTA+AGG - chr3_2:12861713-12861732 Msa0395770:intron 20.0%
!!! GTATTTCTTTTTGAACAATA+TGG - chr3_2:12861798-12861817 Msa0395770:intron 20.0%
!!! ATGAAATTTTTGCAAAACTT+TGG - chr3_2:12862050-12862069 Msa0395770:intron 20.0%
!!! GATTTTAACCATGAAAATTT+TGG - chr3_2:12862498-12862517 Msa0395770:intron 20.0%
!!! AACAAAATCCAAAATTTTCA+TGG + chr3_2:12862509-12862528 None:intergenic 20.0%
!!! AAAAGGTCAACATTTTTTTA+AGG + chr3_2:12863202-12863221 None:intergenic 20.0%
! TACGTATAATTATACGGTTA+CGG - chr3_2:12860704-12860723 Msa0395770:three_prime_UTR 25.0%
! TGACCAAGATAAGATAATTT+GGG - chr3_2:12860752-12860771 Msa0395770:CDS 25.0%
! TTTCCCAAATTATCTTATCT+TGG + chr3_2:12860758-12860777 None:intergenic 25.0%
!! TCTTGTTTAGGGTTATTTAT+TGG - chr3_2:12861202-12861221 Msa0395770:intron 25.0%
!! TTTAGGGTTATTTATTGGTT+TGG - chr3_2:12861207-12861226 Msa0395770:intron 25.0%
!! ATGCATGTTTTTCATTTGTA+TGG + chr3_2:12862449-12862468 None:intergenic 25.0%
!!! AATTTAGTGTGAGTTGTTTT+TGG - chr3_2:12862531-12862550 Msa0395770:intron 25.0%
!!! TGAGTTGTTTTTGGAAATTA+AGG - chr3_2:12862540-12862559 Msa0395770:intron 25.0%
! AAAAGATAGAACATGCAATA+AGG + chr3_2:12862752-12862771 None:intergenic 25.0%
! ATAAATAAACTATGCGGATT+TGG + chr3_2:12862807-12862826 None:intergenic 25.0%
!!! CTATATTTTGAAGCATTATG+CGG - chr3_2:12862849-12862868 Msa0395770:intron 25.0%
! AAATTTCTATCATCAAGTAG+TGG + chr3_2:12863157-12863176 None:intergenic 25.0%
!!! AACATTTTTTTAAGGACAGA+TGG + chr3_2:12863194-12863213 None:intergenic 25.0%
!!! TAACTTTTGAGTATTACGTA+TGG - chr3_2:12863307-12863326 Msa0395770:intron 25.0%
!!! AACTTTTGAGTATTACGTAT+GGG - chr3_2:12863308-12863327 Msa0395770:intron 25.0%
! CATGAAAATCTAAATGACAT+TGG + chr3_2:12863355-12863374 None:intergenic 25.0%
!!! ATTTAAGTTGTTATAGTGAG+AGG + chr3_2:12863392-12863411 None:intergenic 25.0%
!! TTGATGATAATTGAGACTTA+TGG - chr3_2:12863511-12863530 Msa0395770:intron 25.0%
! TGATGATAATTGAGACTTAT+GGG - chr3_2:12863512-12863531 Msa0395770:intron 25.0%
! CTTTCAGAAAACAATTAAGA+AGG - chr3_2:12863726-12863745 Msa0395770:five_prime_UTR 25.0%
! TGTGGTGCAAATAAATTTAA+CGG + chr3_2:12863776-12863795 None:intergenic 25.0%
AGAAAGAGGGAGAAAAAAAA+AGG - chr3_2:12860672-12860691 Msa0395770:three_prime_UTR 30.0%
GTGACCAAGATAAGATAATT+TGG - chr3_2:12860751-12860770 Msa0395770:exon 30.0%
GAGAGTTGTTTATGAAAGAT+GGG + chr3_2:12860874-12860893 None:intergenic 30.0%
TGAGAGTTGTTTATGAAAGA+TGG + chr3_2:12860875-12860894 None:intergenic 30.0%
ATATTTATTCTTTCCAGTGG+CGG + chr3_2:12861061-12861080 None:intergenic 30.0%
ATGCAATTTGGCATGAAATA+TGG - chr3_2:12861410-12861429 Msa0395770:intron 30.0%
CATGAAATATGGTCAAATAG+TGG - chr3_2:12861421-12861440 Msa0395770:intron 30.0%
ACACGATTTAGTATAAAGTG+TGG - chr3_2:12861499-12861518 Msa0395770:intron 30.0%
GTTAAAACTCTTCTTTGCTA+CGG + chr3_2:12861973-12861992 None:intergenic 30.0%
AATGCACATGGCTTAATAAT+AGG - chr3_2:12862115-12862134 Msa0395770:intron 30.0%
AAATATCACGCTATTATGCA+AGG + chr3_2:12862205-12862224 None:intergenic 30.0%
!! TGATATTTTTGTTCTAGTGG+AGG - chr3_2:12862217-12862236 Msa0395770:intron 30.0%
TGCTATGCAATTGTTAAATC+AGG - chr3_2:12862262-12862281 Msa0395770:intron 30.0%
AAACGTATACTCTGCATATT+TGG - chr3_2:12862382-12862401 Msa0395770:intron 30.0%
CATTTGTATGGCTCAAAAAA+TGG + chr3_2:12862437-12862456 None:intergenic 30.0%
!! TTCTTTTGAAAGCTTGCATA+AGG - chr3_2:12862476-12862495 Msa0395770:intron 30.0%
GTTGTGTATATGTCTTTATC+TGG - chr3_2:12862567-12862586 Msa0395770:intron 30.0%
!! TATTTTTGTCGCTCATTGAA+TGG - chr3_2:12862612-12862631 Msa0395770:intron 30.0%
TACATTGCAAGACTAATTAC+AGG + chr3_2:12862650-12862669 None:intergenic 30.0%
ATGCTTCAAAATATAGTTGC+AGG + chr3_2:12862845-12862864 None:intergenic 30.0%
TTCAATTTCCAATGCACTTT+AGG - chr3_2:12862874-12862893 Msa0395770:intron 30.0%
ATATATCACCTAAAGTGCAT+TGG + chr3_2:12862885-12862904 None:intergenic 30.0%
TATGAACTTTAGCTGTTCTA+AGG + chr3_2:12863007-12863026 None:intergenic 30.0%
TTGTGCATTATACACAATAC+AGG + chr3_2:12863076-12863095 None:intergenic 30.0%
TTGTCTGAATTCGGTTATAA+CGG - chr3_2:12863110-12863129 Msa0395770:intron 30.0%
TATTCTAATTCTTTACCTGC+AGG - chr3_2:12863231-12863250 Msa0395770:intron 30.0%
ATTCTAATTCTTTACCTGCA+GGG - chr3_2:12863232-12863251 Msa0395770:intron 30.0%
AATCTAAATGACATTGGCAA+TGG + chr3_2:12863349-12863368 None:intergenic 30.0%
!! CTTAATTGTTTTCTGAAAGC+AGG + chr3_2:12863725-12863744 None:intergenic 30.0%
GTGGTGCAAATAAATTTAAC+GGG + chr3_2:12863775-12863794 None:intergenic 30.0%
TTGGATCTATTTCAGATGTT+TGG - chr3_2:12863826-12863845 Msa0395770:five_prime_UTR 30.0%
GTATTAAGACAATGTATCTC+AGG + chr3_2:12864001-12864020 None:intergenic 30.0%
TGTATAAAGATGTCTGTTTG+TGG + chr3_2:12864025-12864044 None:intergenic 30.0%
AGCTATGTTATTACTGTTTG+TGG + chr3_2:12864048-12864067 None:intergenic 30.0%
ATAATTATACGGTTACGGTG+TGG - chr3_2:12860709-12860728 Msa0395770:three_prime_UTR 35.0%
TAATTATACGGTTACGGTGT+GGG - chr3_2:12860710-12860729 Msa0395770:three_prime_UTR 35.0%
!!! AGTAGCATATTTTGGAGTGT+TGG - chr3_2:12860798-12860817 Msa0395770:CDS 35.0%
GAAGCGAAATTCGAAAAAGA+AGG + chr3_2:12860981-12861000 None:intergenic 35.0%
CGGATATTTATTCTTTCCAG+TGG + chr3_2:12861064-12861083 None:intergenic 35.0%
AAGACACTGTTGACAAACAA+CGG - chr3_2:12861272-12861291 Msa0395770:intron 35.0%
CTGTTGTCAAAGTAGAAATG+CGG + chr3_2:12861928-12861947 None:intergenic 35.0%
CATTTCTACTTTGACAACAG+AGG - chr3_2:12861928-12861947 Msa0395770:intron 35.0%
GTTTCGAAAGATAATGCACA+TGG - chr3_2:12862103-12862122 Msa0395770:intron 35.0%
!! GCGTGATATTTTTGTTCTAG+TGG - chr3_2:12862214-12862233 Msa0395770:intron 35.0%
AATACAGGCACAAAAATCTC+TGG + chr3_2:12863061-12863080 None:intergenic 35.0%
ATTGTGTATAATGCACAAGC+CGG - chr3_2:12863078-12863097 Msa0395770:intron 35.0%
CCGAATTCAGACAAAAGATA+GGG + chr3_2:12863104-12863123 None:intergenic 35.0%
! CCCTATCTTTTGTCTGAATT+CGG - chr3_2:12863101-12863120 Msa0395770:intron 35.0%
ACCGAATTCAGACAAAAGAT+AGG + chr3_2:12863105-12863124 None:intergenic 35.0%
TTTCTATCATCAAGTAGTGG+AGG + chr3_2:12863154-12863173 None:intergenic 35.0%
!! GCATAACTTGTTACAGCTAT+TGG - chr3_2:12863534-12863553 Msa0395770:intron 35.0%
CGAGTTTATTCATGCTTCTA+TGG - chr3_2:12863687-12863706 Msa0395770:intron 35.0%
TCAGCAAATGACAAAGAATG+TGG + chr3_2:12863794-12863813 None:intergenic 35.0%
! TGTCATTTGCTGATATAGTG+TGG - chr3_2:12863801-12863820 Msa0395770:five_prime_UTR 35.0%
! TTGCTGATATAGTGTGGTTT+TGG - chr3_2:12863807-12863826 Msa0395770:five_prime_UTR 35.0%
!!! TCTTTTAAAACGGTCTGAAG+AGG + chr3_2:12863913-12863932 None:intergenic 35.0%
TGTTTAACCGAGTCATGTAT+TGG - chr3_2:12863959-12863978 Msa0395770:five_prime_UTR 35.0%
AATGTGTCCAATACATGACT+CGG + chr3_2:12863969-12863988 None:intergenic 35.0%
AAGAGAGACGGAAAAGTGAA+TGG + chr3_2:12860624-12860643 None:intergenic 40.0%
AGAAGAGGGAATGAGAAAGA+GGG - chr3_2:12860659-12860678 Msa0395770:three_prime_UTR 40.0%
AATTTGGGAAACTGTGTCTG+TGG - chr3_2:12860767-12860786 Msa0395770:CDS 40.0%
! CACGTGAGAGTAGCATATTT+TGG - chr3_2:12860790-12860809 Msa0395770:CDS 40.0%
!! TTAAAGTGGGTTGGACTTTC+GGG + chr3_2:12860849-12860868 None:intergenic 40.0%
!! CTTAAAGTGGGTTGGACTTT+CGG + chr3_2:12860850-12860869 None:intergenic 40.0%
TGAAAGATGGGCCTTAAAGT+GGG + chr3_2:12860862-12860881 None:intergenic 40.0%
ATGAAAGATGGGCCTTAAAG+TGG + chr3_2:12860863-12860882 None:intergenic 40.0%
AAAAAGAAGGCAAACCTGGA+CGG + chr3_2:12860968-12860987 None:intergenic 40.0%
TTCGAAAAAGAAGGCAAACC+TGG + chr3_2:12860972-12860991 None:intergenic 40.0%
AGACACTGTTGACAAACAAC+GGG - chr3_2:12861273-12861292 Msa0395770:intron 40.0%
! CTGGCGCTTTGTTAAAATTC+CGG + chr3_2:12861379-12861398 None:intergenic 40.0%
!! TTAACAAAGCGCCAGTGTTT+TGG - chr3_2:12861384-12861403 Msa0395770:intron 40.0%
!! TAACAAAGCGCCAGTGTTTT+GGG - chr3_2:12861385-12861404 Msa0395770:intron 40.0%
AATTGCATGTCCCAAAACAC+TGG + chr3_2:12861398-12861417 None:intergenic 40.0%
!!! GTGTTTTGGGACATGCAATT+TGG - chr3_2:12861398-12861417 Msa0395770:intron 40.0%
CAATCGAGCATCACTGAATA+TGG + chr3_2:12862002-12862021 None:intergenic 40.0%
! TACCAGTTTTCCTCCTAACT+TGG - chr3_2:12862172-12862191 Msa0395770:intron 40.0%
ATCCAAGTTAGGAGGAAAAC+TGG + chr3_2:12862177-12862196 None:intergenic 40.0%
AGGAAAAGATCCAAGTTAGG+AGG + chr3_2:12862185-12862204 None:intergenic 40.0%
GCAAGGAAAAGATCCAAGTT+AGG + chr3_2:12862188-12862207 None:intergenic 40.0%
GAGTATACGTTTGTCGTTGT+AGG + chr3_2:12862374-12862393 None:intergenic 40.0%
!! TGGCATGTGTTCTAGACTTT+TGG - chr3_2:12862402-12862421 Msa0395770:intron 40.0%
!! GCTCATTGAATGGATTGCTT+CGG - chr3_2:12862622-12862641 Msa0395770:intron 40.0%
!! CTCATTGAATGGATTGCTTC+GGG - chr3_2:12862623-12862642 Msa0395770:intron 40.0%
!! TTTCAGTGTTTCTAGAGACG+TGG - chr3_2:12862707-12862726 Msa0395770:intron 40.0%
ATTCAGACAAAAGATAGGGC+CGG + chr3_2:12863100-12863119 None:intergenic 40.0%
TCTGAATTCGGTTATAACGG+AGG - chr3_2:12863113-12863132 Msa0395770:intron 40.0%
AAGTTGTTATAGTGAGAGGC+AGG + chr3_2:12863388-12863407 None:intergenic 40.0%
!!! ATGAAGTTGGCTTTTCAGCT+TGG + chr3_2:12863588-12863607 None:intergenic 40.0%
CTTCTTCTTTCGACAAGAAG+TGG + chr3_2:12863623-12863642 None:intergenic 40.0%
!! TCAGATGTTTGGTTTGTCCA+AGG - chr3_2:12863837-12863856 Msa0395770:five_prime_UTR 40.0%
TTCAACTTCTTTCGTAGCCT+TGG + chr3_2:12863881-12863900 None:intergenic 40.0%
!! CATGACTCGGTTAAACATAG+TGG + chr3_2:12863956-12863975 None:intergenic 40.0%
TCAATTCGTCGCAAGAGAGA+CGG + chr3_2:12860636-12860655 None:intergenic 45.0%
CGACGAATTGACAGAGAAGA+GGG - chr3_2:12860645-12860664 Msa0395770:three_prime_UTR 45.0%
GAGAAGAGGGAATGAGAAAG+AGG - chr3_2:12860658-12860677 Msa0395770:three_prime_UTR 45.0%
CGAAAGTCCAACCCACTTTA+AGG - chr3_2:12860848-12860867 Msa0395770:intron 45.0%
!! TTTCCGGTGAGTTGAGTTGA+AGG + chr3_2:12860901-12860920 None:intergenic 45.0%
TGTTGACAAACAACGGGTAC+AGG - chr3_2:12861279-12861298 Msa0395770:intron 45.0%
ACACACACCATGCAGAAAGA+CGG - chr3_2:12861683-12861702 Msa0395770:intron 45.0%
TGATGCTCGATTGACTACAC+TGG - chr3_2:12862009-12862028 Msa0395770:intron 45.0%
GCTATTGGAACCTCAGATCA+TGG - chr3_2:12863549-12863568 Msa0395770:intron 45.0%
TTCTGAGTGTCCATGATCTG+AGG + chr3_2:12863562-12863581 None:intergenic 45.0%
GCATGAAGAAGCCTCATTCT+TGG - chr3_2:12863642-12863661 Msa0395770:intron 45.0%
TGAAGAAGCCTCATTCTTGG+AGG - chr3_2:12863645-12863664 Msa0395770:intron 45.0%
CTTCACATCCTCCAAGAATG+AGG + chr3_2:12863656-12863675 None:intergenic 45.0%
CTCATTCTTGGAGGATGTGA+AGG - chr3_2:12863654-12863673 Msa0395770:intron 45.0%
!! GTTTGGTTTGTCCAAGGTAG+TGG - chr3_2:12863843-12863862 Msa0395770:five_prime_UTR 45.0%
GCGACGAATTGACAGAGAAG+AGG - chr3_2:12860644-12860663 Msa0395770:three_prime_UTR 50.0%
AGATGGGCCTTAAAGTGGGT+TGG + chr3_2:12860858-12860877 None:intergenic 50.0%
TCGCCTTCAACTCAACTCAC+CGG - chr3_2:12860895-12860914 Msa0395770:intron 50.0%
AACTCACCGGAAAACTCAGC+CGG - chr3_2:12860908-12860927 Msa0395770:intron 50.0%
! TGAACTCCGGCTGAGTTTTC+CGG + chr3_2:12860917-12860936 None:intergenic 50.0%
! GGAGTTTTCGCTGTTTGTGG+TGG + chr3_2:12860947-12860966 None:intergenic 50.0%
! GACGGAGTTTTCGCTGTTTG+TGG + chr3_2:12860950-12860969 None:intergenic 50.0%
AAACAGCGAAAACTCCGTCC+AGG - chr3_2:12860951-12860970 Msa0395770:CDS 50.0%
TCGGGCGTGTTACAGGAAAA+TGG + chr3_2:12861003-12861022 None:intergenic 50.0%
TCGCGCTTGAATCGCATCAT+CGG + chr3_2:12861022-12861041 None:intergenic 50.0%
TGCGATTCAAGCGCGATTTG+CGG - chr3_2:12861026-12861045 Msa0395770:CDS 50.0%
TGACAAACAACGGGTACAGG+CGG - chr3_2:12861282-12861301 Msa0395770:intron 50.0%
ACTATTGCGCTGTAGCACAC+CGG - chr3_2:12861357-12861376 Msa0395770:intron 50.0%
GCGGCTGCTATTGACAACAA+AGG + chr3_2:12861542-12861561 None:intergenic 50.0%
TGTGTGTGTAGTGGCTGCTT+TGG + chr3_2:12861670-12861689 None:intergenic 50.0%
TCTGCATGGTGTGTGTGTAG+TGG + chr3_2:12861679-12861698 None:intergenic 50.0%
GGCAAAACCGTCTTTCTGCA+TGG + chr3_2:12861693-12861712 None:intergenic 50.0%
CTCACAAATTGGTGTGGTGC+GGG + chr3_2:12861886-12861905 None:intergenic 50.0%
CCTCACAAATTGGTGTGGTG+CGG + chr3_2:12861887-12861906 None:intergenic 50.0%
CACCACACCAATTTGTGAGG+TGG - chr3_2:12861887-12861906 Msa0395770:intron 50.0%
AGTGGAGGCTTCAATCCTTG+TGG + chr3_2:12863139-12863158 None:intergenic 50.0%
AAACACAAGTCATGCCCTGC+AGG + chr3_2:12863249-12863268 None:intergenic 50.0%
GCAACAGCAGCTGATGAAGT+TGG + chr3_2:12863601-12863620 None:intergenic 50.0%
ACTCAGCCGGAGTTCATCGT+CGG - chr3_2:12860921-12860940 Msa0395770:intron 55.0%
CGCGCTTGAATCGCATCATC+GGG + chr3_2:12861021-12861040 None:intergenic 55.0%
! TTTTCCATCGCGGCTAGGAG+CGG + chr3_2:12861606-12861625 None:intergenic 55.0%
CCTAGCCGCGATGGAAAAAC+CGG - chr3_2:12861608-12861627 Msa0395770:intron 55.0%
GAAAAACCGGCCCGCTATTC+AGG - chr3_2:12861621-12861640 Msa0395770:intron 55.0%
AAATTGGTGTGGTGCGGGAG+CGG + chr3_2:12861881-12861900 None:intergenic 55.0%
CCGCACCACACCAATTTGTG+AGG - chr3_2:12861884-12861903 Msa0395770:intron 55.0%
GGCCACCTCACAAATTGGTG+TGG + chr3_2:12861892-12861911 None:intergenic 55.0%
! CGGTTATAACGGAGGCCACA+AGG - chr3_2:12863121-12863140 Msa0395770:intron 55.0%
GTTCGCTTCAGCCACTACCT+TGG + chr3_2:12863857-12863876 None:intergenic 55.0%
AGTGGCTGAAGCGAACTCCA+AGG - chr3_2:12863861-12863880 Msa0395770:five_prime_UTR 55.0%
TGGTGGCCGACGATGAACTC+CGG + chr3_2:12860930-12860949 None:intergenic 60.0%
CGCATCATCGGGCGTGTTAC+AGG + chr3_2:12861010-12861029 None:intergenic 60.0%
TCCGCTCTGCTCCGCTATAG+CGG + chr3_2:12861570-12861589 None:intergenic 60.0%
! CCGGTTTTTCCATCGCGGCT+AGG + chr3_2:12861611-12861630 None:intergenic 60.0%
!! GCGGGCGCTATTTTGTGGCA+CGG + chr3_2:12861850-12861869 None:intergenic 60.0%
!!! GCGTAGCGGGCGCTATTTTG+TGG + chr3_2:12861855-12861874 None:intergenic 60.0%
AATTGGTGTGGTGCGGGAGC+GGG + chr3_2:12861880-12861899 None:intergenic 60.0%
AGTAGAAATGCGGCGCAGCG+CGG + chr3_2:12861918-12861937 None:intergenic 60.0%
GCGGCTTCGATAACCGCCAC+TGG - chr3_2:12861045-12861064 Msa0395770:CDS 65.0%
CCGCTCTGCTCCGCTATAGC+GGG + chr3_2:12861569-12861588 None:intergenic 65.0%
! CCCGCTATAGCGGAGCAGAG+CGG - chr3_2:12861566-12861585 Msa0395770:intron 65.0%
!! GCGGGCCGGTTTTTCCATCG+CGG + chr3_2:12861616-12861635 None:intergenic 65.0%
!! CGCACTCCTGAATAGCGGGC+CGG + chr3_2:12861630-12861649 None:intergenic 65.0%
CGGCCCGCTATTCAGGAGTG+CGG - chr3_2:12861628-12861647 Msa0395770:intron 65.0%
! GGCCCGCTATTCAGGAGTGC+GGG - chr3_2:12861629-12861648 Msa0395770:intron 65.0%
! GCCCGCTATTCAGGAGTGCG+GGG - chr3_2:12861630-12861649 Msa0395770:intron 65.0%
CCCCCGCACTCCTGAATAGC+GGG + chr3_2:12861634-12861653 None:intergenic 65.0%
! CCCGCTATTCAGGAGTGCGG+GGG - chr3_2:12861631-12861650 Msa0395770:intron 65.0%
CCCCCCGCACTCCTGAATAG+CGG + chr3_2:12861635-12861654 None:intergenic 65.0%
! CCGCTATTCAGGAGTGCGGG+GGG - chr3_2:12861632-12861651 Msa0395770:intron 65.0%
ATTGGTGTGGTGCGGGAGCG+GGG + chr3_2:12861879-12861898 None:intergenic 65.0%
GGCGCGGCCACCTCACAAAT+TGG + chr3_2:12861897-12861916 None:intergenic 65.0%
AGCCGCGCTGCCCGCTATAG+CGG - chr3_2:12861556-12861575 Msa0395770:intron 70.0%
!! CTCCGCTATAGCGGGCAGCG+CGG + chr3_2:12861561-12861580 None:intergenic 70.0%
CCATCGCGGCTAGGAGCGGT+GGG + chr3_2:12861602-12861621 None:intergenic 70.0%
TCCATCGCGGCTAGGAGCGG+TGG + chr3_2:12861603-12861622 None:intergenic 70.0%
CCCACCGCTCCTAGCCGCGA+TGG - chr3_2:12861599-12861618 Msa0395770:intron 75.0%
TGCGGGAGCGGGGTGCGTAG+CGG + chr3_2:12861869-12861888 None:intergenic 75.0%
AAATGCGGCGCAGCGCGGCG+CGG + chr3_2:12861913-12861932 None:intergenic 75.0%
! GCGGGAGCGGGGTGCGTAGC+GGG + chr3_2:12861868-12861887 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 12860606 12864082 12860606 ID=Msa0395770;Name=Msa0395770
chr3_2 mRNA 12860606 12864082 12860606 ID=Msa0395770-mRNA-1;Parent=Msa0395770;Name=Msa0395770-mRNA-1;_AED=0.06;_eAED=0.06;_QI=385|1|1|1|0.5|0.33|3|146|91
chr3_2 exon 12860606 12860847 12860606 ID=Msa0395770-mRNA-1:exon:3026;Parent=Msa0395770-mRNA-1
chr3_2 exon 12860941 12861138 12860941 ID=Msa0395770-mRNA-1:exon:3025;Parent=Msa0395770-mRNA-1
chr3_2 exon 12863716 12864082 12863716 ID=Msa0395770-mRNA-1:exon:3024;Parent=Msa0395770-mRNA-1
chr3_2 five_prime_UTR 12863716 12864082 12863716 ID=Msa0395770-mRNA-1:five_prime_utr;Parent=Msa0395770-mRNA-1
chr3_2 five_prime_UTR 12861121 12861138 12861121 ID=Msa0395770-mRNA-1:five_prime_utr;Parent=Msa0395770-mRNA-1
chr3_2 CDS 12860941 12861120 12860941 ID=Msa0395770-mRNA-1:cds;Parent=Msa0395770-mRNA-1
chr3_2 CDS 12860752 12860847 12860752 ID=Msa0395770-mRNA-1:cds;Parent=Msa0395770-mRNA-1
chr3_2 three_prime_UTR 12860606 12860751 12860606 ID=Msa0395770-mRNA-1:three_prime_utr;Parent=Msa0395770-mRNA-1
Gene Sequence

>Msa0395770

ATGGACACTCAGAAAAGCCAAGCTGAAAAGCCAACTTCATCAGCTGCTGTTGCCACTTCTTGTCGAAAGAAGAAGCATGAAGAAGCCTCATTCTTGGAGGATGTGAAGGATCACATTGACGAGTTTATTCATGCTTCTATGGATGAACACAAAACCTGCTTTCAGAAAACAATTAAGAAGATGTTTGGTTTGTCCAAGGTAGTGGCTGAAGCGAACTCCAAGGCTACGAAAGAAGTTGAAAGTTCTTTGCCTCTTCAGACCGTTTTAAAAGAGTAA
Protein sequence

>Msa0395770

MDTQKSQAEKPTSSAAVATSCRKKKHEEASFLEDVKDHIDEFIHASMDEHKTCFQKTIKKMFGLSKVVAEANSKATKEVESSLPLQTVLKE*