AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0401790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0401790 MtrunA17_Chr5g0399151 77.273 44 10 0 27 70 267 310 1.23e-14 67.0
Msa0401790 MtrunA17_Chr4g0072141 84.375 32 5 0 1 32 98 129 2.28e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTAGATCTAAAGGCTTAA+AGG 0.218793 3_2:+28652275 Msa0401790:intron
ATCTAAAGGCTTAAAGGCTT+TGG 0.275121 3_2:+28652281 Msa0401790:intron
TTTATTATTAATGCTGGTTC+TGG 0.287893 3_2:+28651879 Msa0401790:CDS
ATGATAAGAGCTTACGCTTT+GGG 0.347397 3_2:-28652333 None:intergenic
AAAATCAAATATCATGCAAA+TGG 0.367440 3_2:+28651566 Msa0401790:CDS
TCTTCTGAGACTATTTCAAT+AGG 0.407520 3_2:-28651602 None:intergenic
AAATCAAATATCATGCAAAT+GGG 0.412981 3_2:+28651567 Msa0401790:CDS
AAATTCTTTATTATTAATGC+TGG 0.441394 3_2:+28651873 Msa0401790:CDS
AATGATAAGAGCTTACGCTT+TGG 0.453757 3_2:-28652334 None:intergenic
TGTATCAACCTCTAGCTGAC+AGG 0.505962 3_2:+28652035 Msa0401790:intron
TTGCTACCGTGCGTTCACTT+AGG 0.512623 3_2:-28651935 None:intergenic
GTTCTGGCTTTATGATGTTG+CGG 0.558128 3_2:+28651895 Msa0401790:CDS
GATGTTGCGGAAAGCGGTGA+AGG 0.561562 3_2:+28651908 Msa0401790:CDS
ATGGTTCACGAACTCACAAA+GGG 0.580731 3_2:+28651536 Msa0401790:CDS
GGCATTCCTAAGTGAACGCA+CGG 0.591999 3_2:+28651929 Msa0401790:CDS
TGTTGAGTTTGCAACTGCAG+AGG 0.598820 3_2:+28652307 Msa0401790:CDS
CTTTATGATGTTGCGGAAAG+CGG 0.613550 3_2:+28651902 Msa0401790:CDS
AATCAAATATCATGCAAATG+GGG 0.623882 3_2:+28651568 Msa0401790:CDS
TATGGTTCACGAACTCACAA+AGG 0.632329 3_2:+28651535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATCAACTTCAATA+GGG - chr3_2:28651827-28651846 None:intergenic 15.0%
!! AAATTCTTTATTATTAATGC+TGG + chr3_2:28651873-28651892 Msa0401790:CDS 15.0%
!!! AATTTTAATCAACTTCAATA+GGG - chr3_2:28651827-28651846 None:intergenic 15.0%
!! AAATTCTTTATTATTAATGC+TGG + chr3_2:28651873-28651892 Msa0401790:CDS 15.0%
!! AAAATCAAATATCATGCAAA+TGG + chr3_2:28651566-28651585 Msa0401790:CDS 20.0%
!! AAATCAAATATCATGCAAAT+GGG + chr3_2:28651567-28651586 Msa0401790:CDS 20.0%
!!! GAATTTTAATCAACTTCAAT+AGG - chr3_2:28651828-28651847 None:intergenic 20.0%
!! AAAATCAAATATCATGCAAA+TGG + chr3_2:28651566-28651585 Msa0401790:CDS 20.0%
!! AAATCAAATATCATGCAAAT+GGG + chr3_2:28651567-28651586 Msa0401790:CDS 20.0%
!!! GAATTTTAATCAACTTCAAT+AGG - chr3_2:28651828-28651847 None:intergenic 20.0%
! AATCAAATATCATGCAAATG+GGG + chr3_2:28651568-28651587 Msa0401790:CDS 25.0%
!!! TTTATTATTAATGCTGGTTC+TGG + chr3_2:28651879-28651898 Msa0401790:CDS 25.0%
!! ATACAAAATTCGTTTTACCT+AGG - chr3_2:28652144-28652163 None:intergenic 25.0%
! AGTAACATATTCATCAGAAA+AGG - chr3_2:28652211-28652230 None:intergenic 25.0%
! AATGAAACTCTTAGATCTAA+AGG + chr3_2:28652267-28652286 Msa0401790:intron 25.0%
! AATCAAATATCATGCAAATG+GGG + chr3_2:28651568-28651587 Msa0401790:CDS 25.0%
!!! TTTATTATTAATGCTGGTTC+TGG + chr3_2:28651879-28651898 Msa0401790:CDS 25.0%
!! ATACAAAATTCGTTTTACCT+AGG - chr3_2:28652144-28652163 None:intergenic 25.0%
! AGTAACATATTCATCAGAAA+AGG - chr3_2:28652211-28652230 None:intergenic 25.0%
! AATGAAACTCTTAGATCTAA+AGG + chr3_2:28652267-28652286 Msa0401790:intron 25.0%
!! TCTTCTGAGACTATTTCAAT+AGG - chr3_2:28651605-28651624 None:intergenic 30.0%
TGATAGCAATTATTATCCTG+AGG + chr3_2:28651626-28651645 Msa0401790:intron 30.0%
CTTAATCACACATTAATAGC+AGG - chr3_2:28651700-28651719 None:intergenic 30.0%
CAACAAAATATAGTTGTGCA+AGG - chr3_2:28652017-28652036 None:intergenic 30.0%
AGTTGCTACTGAAAAAATCA+TGG - chr3_2:28652175-28652194 None:intergenic 30.0%
!!! GATTTTTTCAGTAGCAACTT+AGG + chr3_2:28652176-28652195 Msa0401790:intron 30.0%
TCACAAATAGATCTGATTGT+AGG - chr3_2:28652235-28652254 None:intergenic 30.0%
TCTTAGATCTAAAGGCTTAA+AGG + chr3_2:28652275-28652294 Msa0401790:intron 30.0%
!! TCTTCTGAGACTATTTCAAT+AGG - chr3_2:28651605-28651624 None:intergenic 30.0%
TGATAGCAATTATTATCCTG+AGG + chr3_2:28651626-28651645 Msa0401790:intron 30.0%
CTTAATCACACATTAATAGC+AGG - chr3_2:28651700-28651719 None:intergenic 30.0%
CAACAAAATATAGTTGTGCA+AGG - chr3_2:28652017-28652036 None:intergenic 30.0%
AGTTGCTACTGAAAAAATCA+TGG - chr3_2:28652175-28652194 None:intergenic 30.0%
!!! GATTTTTTCAGTAGCAACTT+AGG + chr3_2:28652176-28652195 Msa0401790:intron 30.0%
TCACAAATAGATCTGATTGT+AGG - chr3_2:28652235-28652254 None:intergenic 30.0%
TCTTAGATCTAAAGGCTTAA+AGG + chr3_2:28652275-28652294 Msa0401790:intron 30.0%
!! TGATCAACAAAAAGTACCTC+AGG - chr3_2:28651645-28651664 None:intergenic 35.0%
CGGTAGCAAAAATTCAAGTA+CGG + chr3_2:28651949-28651968 Msa0401790:intron 35.0%
AAAACCTGAAACAGGAATGT+TGG - chr3_2:28652081-28652100 None:intergenic 35.0%
! TTATCTCAATGTGTTGCTAG+AGG + chr3_2:28652110-28652129 Msa0401790:intron 35.0%
ATCTAAAGGCTTAAAGGCTT+TGG + chr3_2:28652281-28652300 Msa0401790:intron 35.0%
!! TGATCAACAAAAAGTACCTC+AGG - chr3_2:28651645-28651664 None:intergenic 35.0%
CGGTAGCAAAAATTCAAGTA+CGG + chr3_2:28651949-28651968 Msa0401790:intron 35.0%
AAAACCTGAAACAGGAATGT+TGG - chr3_2:28652081-28652100 None:intergenic 35.0%
! TTATCTCAATGTGTTGCTAG+AGG + chr3_2:28652110-28652129 Msa0401790:intron 35.0%
ATCTAAAGGCTTAAAGGCTT+TGG + chr3_2:28652281-28652300 Msa0401790:intron 35.0%
ATGGTTCACGAACTCACAAA+GGG + chr3_2:28651536-28651555 Msa0401790:CDS 40.0%
!! GTTCTGGCTTTATGATGTTG+CGG + chr3_2:28651895-28651914 Msa0401790:CDS 40.0%
CTTTATGATGTTGCGGAAAG+CGG + chr3_2:28651902-28651921 Msa0401790:CDS 40.0%
ATCTCCAACATTCCTGTTTC+AGG + chr3_2:28652074-28652093 Msa0401790:intron 40.0%
TAGAACGCAAAACCTGAAAC+AGG - chr3_2:28652089-28652108 None:intergenic 40.0%
ATGGTTCACGAACTCACAAA+GGG + chr3_2:28651536-28651555 Msa0401790:CDS 40.0%
!! GTTCTGGCTTTATGATGTTG+CGG + chr3_2:28651895-28651914 Msa0401790:CDS 40.0%
CTTTATGATGTTGCGGAAAG+CGG + chr3_2:28651902-28651921 Msa0401790:CDS 40.0%
ATCTCCAACATTCCTGTTTC+AGG + chr3_2:28652074-28652093 Msa0401790:intron 40.0%
TAGAACGCAAAACCTGAAAC+AGG - chr3_2:28652089-28652108 None:intergenic 40.0%
TGTATCAACCTCTAGCTGAC+AGG + chr3_2:28652035-28652054 Msa0401790:intron 45.0%
AACACAAACCTGTCAGCTAG+AGG - chr3_2:28652046-28652065 None:intergenic 45.0%
TGCTAGAGGCTATTGATCCT+AGG + chr3_2:28652124-28652143 Msa0401790:intron 45.0%
TGTTGAGTTTGCAACTGCAG+AGG + chr3_2:28652307-28652326 Msa0401790:CDS 45.0%
TGTATCAACCTCTAGCTGAC+AGG + chr3_2:28652035-28652054 Msa0401790:intron 45.0%
AACACAAACCTGTCAGCTAG+AGG - chr3_2:28652046-28652065 None:intergenic 45.0%
TGCTAGAGGCTATTGATCCT+AGG + chr3_2:28652124-28652143 Msa0401790:intron 45.0%
TGTTGAGTTTGCAACTGCAG+AGG + chr3_2:28652307-28652326 Msa0401790:CDS 45.0%
GGCATTCCTAAGTGAACGCA+CGG + chr3_2:28651929-28651948 Msa0401790:CDS 50.0%
TTGCTACCGTGCGTTCACTT+AGG - chr3_2:28651938-28651957 None:intergenic 50.0%
GGCATTCCTAAGTGAACGCA+CGG + chr3_2:28651929-28651948 Msa0401790:CDS 50.0%
TTGCTACCGTGCGTTCACTT+AGG - chr3_2:28651938-28651957 None:intergenic 50.0%
GATGTTGCGGAAAGCGGTGA+AGG + chr3_2:28651908-28651927 Msa0401790:CDS 55.0%
GATGTTGCGGAAAGCGGTGA+AGG + chr3_2:28651908-28651927 Msa0401790:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 28651536 28652344 28651536 ID=Msa0401790;Name=Msa0401790
chr3_2 mRNA 28651536 28652344 28651536 ID=Msa0401790-mRNA-1;Parent=Msa0401790;Name=Msa0401790-mRNA-1;_AED=0.59;_eAED=0.77;_QI=0|0|0|1|0|0.5|4|0|80
chr3_2 exon 28651536 28651613 28651536 ID=Msa0401790-mRNA-1:exon:5834;Parent=Msa0401790-mRNA-1
chr3_2 exon 28651864 28651965 28651864 ID=Msa0401790-mRNA-1:exon:5835;Parent=Msa0401790-mRNA-1
chr3_2 exon 28652050 28652056 28652050 ID=Msa0401790-mRNA-1:exon:5836;Parent=Msa0401790-mRNA-1
chr3_2 exon 28652289 28652344 28652289 ID=Msa0401790-mRNA-1:exon:5837;Parent=Msa0401790-mRNA-1
chr3_2 CDS 28651536 28651613 28651536 ID=Msa0401790-mRNA-1:cds;Parent=Msa0401790-mRNA-1
chr3_2 CDS 28651864 28651965 28651864 ID=Msa0401790-mRNA-1:cds;Parent=Msa0401790-mRNA-1
chr3_2 CDS 28652050 28652056 28652050 ID=Msa0401790-mRNA-1:cds;Parent=Msa0401790-mRNA-1
chr3_2 CDS 28652289 28652344 28652289 ID=Msa0401790-mRNA-1:cds;Parent=Msa0401790-mRNA-1
Gene Sequence

>Msa0401790

ATGGTTCACGAACTCACAAAGGGAAGCTATAAAATCAAATATCATGCAAATGGGGTTGATAAAGATCCTATTGAAATAACTCTAAATAAATTCTTTATTATTAATGCTGGTTCTGGCTTTATGATGTTGCGGAAAGCGGTGAAGGCATTCCTAAGTGAACGCACGGTAGCAAAAATTCAACTGACAGGCTTAAAGGCTTTGGACATGTTGAGTTTGCAACTGCAGAGGCAGCCCAAAGCGTAA

Protein sequence

>Msa0401790

MVHELTKGSYKIKYHANGVDKDPIEITLNKFFIINAGSGFMMLRKAVKAFLSERTVAKIQLTGLKALDMLSLQLQRQPKA*