AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603410 MtrunA17_Chr4g0031671 91.781 146 2 1 1 136 1 146 1.41e-88 254
Msa0603410 MtrunA17_Chr4g0031631 91.781 146 2 1 1 136 1 146 1.41e-88 254
Msa0603410 MtrunA17_Chr4g0030941 91.781 146 2 1 1 136 1 146 1.41e-88 254
Msa0603410 MtrunA17_Chr4g0035061 91.781 146 2 1 1 136 1 146 1.73e-88 254
Msa0603410 MtrunA17_Chr4g0035631 91.781 146 2 1 1 136 1 146 1.73e-88 254
Msa0603410 MtrunA17_Chr4g0031061 91.096 146 3 1 1 136 1 146 8.40e-88 253
Msa0603410 MtrunA17_Chr4g0032321 91.096 146 3 1 1 136 1 146 8.40e-88 253
Msa0603410 MtrunA17_Chr4g0035071 90.411 146 4 1 1 136 1 146 7.45e-87 250
Msa0603410 MtrunA17_Chr4g0030981 91.096 146 2 2 1 136 1 145 2.30e-86 249
Msa0603410 MtrunA17_Chr4g0030951 91.549 142 2 1 5 136 4 145 1.27e-85 247
Msa0603410 MtrunA17_Chr4g0046191 87.671 146 3 2 1 136 1 141 5.51e-76 223
Msa0603410 MtrunA17_Chr1g0197451 82.192 146 5 4 1 136 1 135 1.25e-65 196
Msa0603410 MtrunA17_Chr1g0198251 81.507 146 6 4 1 136 1 135 4.49e-65 195
Msa0603410 MtrunA17_Chr1g0182361 98.734 79 1 0 58 136 56 134 1.81e-52 163
Msa0603410 MtrunA17_Chr7g0263031 98.734 79 1 0 58 136 58 136 2.09e-52 163
Msa0603410 MtrunA17_Chr2g0320511 65.385 78 27 0 58 135 145 222 4.21e-32 114
Msa0603410 MtrunA17_Chr4g0031051 90.196 51 5 0 76 126 1 51 6.71e-27 95.9
Msa0603410 MtrunA17_Chr8g0368781 58.065 93 21 4 15 97 4 88 1.31e-22 86.3
Msa0603410 MtrunA17_Chr7g0228831 93.103 29 2 0 58 86 53 81 1.76e-13 62.8
Msa0603410 MtrunA17_Chr4g0075141 37.647 85 51 2 58 141 38 121 9.38e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603410 AT3G45980.1 81.879 149 13 3 1 136 1 148 1.57e-70 209
Msa0603410 AT2G28720.1 79.866 149 17 2 1 136 1 149 2.13e-68 204
Msa0603410 AT1G07790.1 77.397 146 23 3 1 136 1 146 1.27e-66 199
Msa0603410 AT5G59910.1 83.582 134 9 3 13 136 18 148 3.48e-64 193
Msa0603410 AT3G46030.1 86.290 124 6 2 23 136 21 143 1.27e-62 189
Msa0603410 AT2G37470.1 74.483 145 20 4 1 136 1 137 1.23e-59 181
Msa0603410 AT3G53650.1 77.083 144 17 3 1 136 1 136 3.19e-59 181
Msa0603410 AT5G02570.1 73.288 146 13 2 1 136 1 130 2.04e-58 178
Msa0603410 AT3G09480.1 78.333 120 20 1 23 136 5 124 8.53e-56 171
Msa0603410 AT5G22880.1 79.720 143 17 3 4 136 3 143 8.25e-54 167
Msa0603410 AT1G08170.1 58.889 90 36 1 46 135 147 235 8.31e-34 119

Find 48 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATAATTGTGGAATTTAA+TGG 0.299216 4_2:+47922757 Msa0603410:CDS
GGAAGCTTCTTCCCTGCCTT+TGG 0.302255 4_2:-47922279 None:intergenic
CTTCTCCGCCGGTGCTTTCT+CGG 0.338411 4_2:-47922253 None:intergenic
ACTTGTTCTTCCCGGAGAAT+TGG 0.361228 4_2:+47922554 Msa0603410:CDS
GCTGGCTTCTTCTCTCCCTT+TGG 0.368494 4_2:-47922192 None:intergenic
GGCAGCAGAACCACCTTCCT+TGG 0.388400 4_2:-47922301 None:intergenic
GCTGTCAGACTTGTTCTTCC+CGG 0.392312 4_2:+47922546 Msa0603410:CDS
GCCTTTGGCTTCTTCTCCGC+CGG 0.406575 4_2:-47922264 None:intergenic
AGCATGCTTGGCCAATTCTC+CGG 0.411273 4_2:-47922565 None:intergenic
AGTAAACTTGGTCACAGCTT+TGG 0.428250 4_2:-47922601 None:intergenic
ATTTCCCTGGAAGTGATTGT+TGG 0.430207 4_2:-47922516 None:intergenic
GTGGACTTCTTCTCCTCTGC+GGG 0.437960 4_2:-47922225 None:intergenic
AAGGAAGGTGGTTCTGCTGC+CGG 0.454682 4_2:+47922303 Msa0603410:CDS
GCGGAGAAGAAGCCAAAGGC+AGG 0.456047 4_2:+47922267 Msa0603410:CDS
ACCCTCAGAAACAGCATGCT+TGG 0.462043 4_2:-47922577 None:intergenic
TTGCTTGAGATACCAATGTC+CGG 0.465790 4_2:-47922405 None:intergenic
TCTGCGGGTTTCTTCTCCGC+TGG 0.466008 4_2:-47922210 None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG 0.474508 4_2:-47922322 None:intergenic
AGAAGCCAACAATCACTTCC+AGG 0.480188 4_2:+47922511 Msa0603410:CDS
CGCCGGTGCTTTCTCGGCGA+CGG 0.489239 4_2:-47922247 None:intergenic
GACAGCAGTCTGAATTTCCC+TGG 0.496873 4_2:-47922529 None:intergenic
CAAATTCTCAATGGCACCAA+AGG 0.499774 4_2:+47922176 None:intergenic
GCAGCAGAACCACCTTCCTT+GGG 0.503667 4_2:-47922300 None:intergenic
GCATGCTTGGCCAATTCTCC+GGG 0.507742 4_2:-47922564 None:intergenic
TGCTTGAGATACCAATGTCC+GGG 0.518516 4_2:-47922404 None:intergenic
AATCTAAGAACTAGTAAACT+TGG 0.523030 4_2:-47922613 None:intergenic
TGTAATGAAGTCATGCAATC+TGG 0.526440 4_2:+47922719 Msa0603410:CDS
TGGTATCTCAAGCAAAGCCA+TGG 0.533917 4_2:+47922413 Msa0603410:CDS
GCCAAGCATGCTGTTTCTGA+GGG 0.538001 4_2:+47922576 Msa0603410:CDS
GGCCAAGCATGCTGTTTCTG+AGG 0.538906 4_2:+47922575 Msa0603410:CDS
AAATTCTCAATGGCACCAAA+GGG 0.562232 4_2:+47922177 None:intergenic
CGGAGAAGAAGCCAAAGGCA+GGG 0.564014 4_2:+47922268 Msa0603410:CDS
AAACAAGTTCACCCGGACAT+TGG 0.567029 4_2:+47922393 Msa0603410:CDS
CACCGTCGCCGAGAAAGCAC+CGG 0.573263 4_2:+47922245 Msa0603410:CDS
GGGAAGAAGCTTCCCAAGGA+AGG 0.587709 4_2:+47922288 Msa0603410:CDS
ACCGGCGGAGAAGAAGCCAA+AGG 0.599875 4_2:+47922263 Msa0603410:CDS
GGTTCTTAAACAAGTTCACC+CGG 0.632369 4_2:+47922386 Msa0603410:intron
AAGAAGCTTCCCAAGGAAGG+TGG 0.636715 4_2:+47922291 Msa0603410:CDS
GGCAGGGAAGAAGCTTCCCA+AGG 0.638223 4_2:+47922284 Msa0603410:CDS
CGTCGCCGAGAAAGCACCGG+CGG 0.638962 4_2:+47922248 Msa0603410:CDS
GAAGCCAACAATCACTTCCA+GGG 0.639164 4_2:+47922512 Msa0603410:CDS
AAAGGGAGAGAAGAAGCCAG+CGG 0.642633 4_2:+47922194 Msa0603410:CDS
CGGTGCTTTCTCGGCGACGG+TGG 0.648960 4_2:-47922244 None:intergenic
GGTATCTCAAGCAAAGCCAT+GGG 0.659631 4_2:+47922414 Msa0603410:CDS
ATGAAGTCATGCAATCTGGT+TGG 0.660641 4_2:+47922723 Msa0603410:CDS
GGTGGACTTCTTCTCCTCTG+CGG 0.668086 4_2:-47922226 None:intergenic
AGCGGAGAAGAAACCCGCAG+AGG 0.676003 4_2:+47922212 Msa0603410:CDS
GAAACTGTTCATGATACCCA+TGG 0.683953 4_2:-47922430 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAATTGTGGAATTTAA+TGG + chr4_2:47922757-47922776 Msa0603410:CDS 20.0%
! AATCTAAGAACTAGTAAACT+TGG - chr4_2:47922616-47922635 None:intergenic 25.0%
!! TCTTAGATTTGTAACACTTT+TGG + chr4_2:47922627-47922646 Msa0603410:intron 25.0%
! CTGCTAATTAAAATTACCAA+TGG - chr4_2:47922673-47922692 None:intergenic 25.0%
!! TCTTTTGTGTATGATAATTG+TGG + chr4_2:47922747-47922766 Msa0603410:CDS 25.0%
ATACAAGATCTACATCTTCA+AGG + chr4_2:47922365-47922384 Msa0603410:intron 30.0%
!! GATTTGTAACACTTTTGGTT+TGG + chr4_2:47922632-47922651 Msa0603410:intron 30.0%
!!! TTTGGTTTGGATCTAGTTTT+AGG + chr4_2:47922645-47922664 Msa0603410:intron 30.0%
!!! GATCTAGTTTTAGGAACCAT+TGG + chr4_2:47922654-47922673 Msa0603410:intron 35.0%
TGTAATGAAGTCATGCAATC+TGG + chr4_2:47922719-47922738 Msa0603410:CDS 35.0%
TCTCTTCTTCTTCTTGTCTC+CGG - chr4_2:47922325-47922344 None:intergenic 40.0%
GGTTCTTAAACAAGTTCACC+CGG + chr4_2:47922386-47922405 Msa0603410:intron 40.0%
TTGCTTGAGATACCAATGTC+CGG - chr4_2:47922408-47922427 None:intergenic 40.0%
GAAACTGTTCATGATACCCA+TGG - chr4_2:47922433-47922452 None:intergenic 40.0%
ATTTCCCTGGAAGTGATTGT+TGG - chr4_2:47922519-47922538 None:intergenic 40.0%
AGTAAACTTGGTCACAGCTT+TGG - chr4_2:47922604-47922623 None:intergenic 40.0%
ATGAAGTCATGCAATCTGGT+TGG + chr4_2:47922723-47922742 Msa0603410:CDS 40.0%
AAACAAGTTCACCCGGACAT+TGG + chr4_2:47922393-47922412 Msa0603410:CDS 45.0%
TGCTTGAGATACCAATGTCC+GGG - chr4_2:47922407-47922426 None:intergenic 45.0%
TGGTATCTCAAGCAAAGCCA+TGG + chr4_2:47922413-47922432 Msa0603410:CDS 45.0%
GGTATCTCAAGCAAAGCCAT+GGG + chr4_2:47922414-47922433 Msa0603410:CDS 45.0%
AGAAGCCAACAATCACTTCC+AGG + chr4_2:47922511-47922530 Msa0603410:CDS 45.0%
GAAGCCAACAATCACTTCCA+GGG + chr4_2:47922512-47922531 Msa0603410:CDS 45.0%
ACTTGTTCTTCCCGGAGAAT+TGG + chr4_2:47922554-47922573 Msa0603410:CDS 45.0%
AAAGGGAGAGAAGAAGCCAG+CGG + chr4_2:47922194-47922213 Msa0603410:CDS 50.0%
AAGAAGCTTCCCAAGGAAGG+TGG + chr4_2:47922291-47922310 Msa0603410:CDS 50.0%
GACAGCAGTCTGAATTTCCC+TGG - chr4_2:47922532-47922551 None:intergenic 50.0%
! GCTGTCAGACTTGTTCTTCC+CGG + chr4_2:47922546-47922565 Msa0603410:CDS 50.0%
AGCATGCTTGGCCAATTCTC+CGG - chr4_2:47922568-47922587 None:intergenic 50.0%
!! GCCAAGCATGCTGTTTCTGA+GGG + chr4_2:47922576-47922595 Msa0603410:CDS 50.0%
ACCCTCAGAAACAGCATGCT+TGG - chr4_2:47922580-47922599 None:intergenic 50.0%
! GCTGGCTTCTTCTCTCCCTT+TGG - chr4_2:47922195-47922214 None:intergenic 55.0%
GTGGACTTCTTCTCCTCTGC+GGG - chr4_2:47922228-47922247 None:intergenic 55.0%
! GGTGGACTTCTTCTCCTCTG+CGG - chr4_2:47922229-47922248 None:intergenic 55.0%
CGGAGAAGAAGCCAAAGGCA+GGG + chr4_2:47922268-47922287 Msa0603410:CDS 55.0%
GGAAGCTTCTTCCCTGCCTT+TGG - chr4_2:47922282-47922301 None:intergenic 55.0%
GGGAAGAAGCTTCCCAAGGA+AGG + chr4_2:47922288-47922307 Msa0603410:CDS 55.0%
GCAGCAGAACCACCTTCCTT+GGG - chr4_2:47922303-47922322 None:intergenic 55.0%
! AAGGAAGGTGGTTCTGCTGC+CGG + chr4_2:47922303-47922322 Msa0603410:CDS 55.0%
GCATGCTTGGCCAATTCTCC+GGG - chr4_2:47922567-47922586 None:intergenic 55.0%
!! GGCCAAGCATGCTGTTTCTG+AGG + chr4_2:47922575-47922594 Msa0603410:CDS 55.0%
TCTGCGGGTTTCTTCTCCGC+TGG - chr4_2:47922213-47922232 None:intergenic 60.0%
AGCGGAGAAGAAACCCGCAG+AGG + chr4_2:47922212-47922231 Msa0603410:CDS 60.0%
CTTCTCCGCCGGTGCTTTCT+CGG - chr4_2:47922256-47922275 None:intergenic 60.0%
ACCGGCGGAGAAGAAGCCAA+AGG + chr4_2:47922263-47922282 Msa0603410:CDS 60.0%
! GCCTTTGGCTTCTTCTCCGC+CGG - chr4_2:47922267-47922286 None:intergenic 60.0%
GCGGAGAAGAAGCCAAAGGC+AGG + chr4_2:47922267-47922286 Msa0603410:CDS 60.0%
GGCAGGGAAGAAGCTTCCCA+AGG + chr4_2:47922284-47922303 Msa0603410:CDS 60.0%
GGCAGCAGAACCACCTTCCT+TGG - chr4_2:47922304-47922323 None:intergenic 60.0%
! CACCGTCGCCGAGAAAGCAC+CGG + chr4_2:47922245-47922264 Msa0603410:CDS 65.0%
CGGTGCTTTCTCGGCGACGG+TGG - chr4_2:47922247-47922266 None:intergenic 70.0%
CGCCGGTGCTTTCTCGGCGA+CGG - chr4_2:47922250-47922269 None:intergenic 70.0%
!! CGTCGCCGAGAAAGCACCGG+CGG + chr4_2:47922248-47922267 Msa0603410:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 47922186 47922784 47922186 ID=Msa0603410;Name=Msa0603410
chr4_2 mRNA 47922186 47922784 47922186 ID=Msa0603410-mRNA-1;Parent=Msa0603410;Name=Msa0603410-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0.33|1|0|0|3|8|158
chr4_2 exon 47922186 47922356 47922186 ID=Msa0603410-mRNA-1:exon:10995;Parent=Msa0603410-mRNA-1
chr4_2 exon 47922387 47922624 47922387 ID=Msa0603410-mRNA-1:exon:10996;Parent=Msa0603410-mRNA-1
chr4_2 exon 47922709 47922784 47922709 ID=Msa0603410-mRNA-1:exon:10997;Parent=Msa0603410-mRNA-1
chr4_2 CDS 47922186 47922356 47922186 ID=Msa0603410-mRNA-1:cds;Parent=Msa0603410-mRNA-1
chr4_2 CDS 47922387 47922624 47922387 ID=Msa0603410-mRNA-1:cds;Parent=Msa0603410-mRNA-1
chr4_2 CDS 47922709 47922776 47922709 ID=Msa0603410-mRNA-1:cds;Parent=Msa0603410-mRNA-1
chr4_2 three_prime_UTR 47922777 47922784 47922777 ID=Msa0603410-mRNA-1:three_prime_utr;Parent=Msa0603410-mRNA-1
Gene Sequence

>Msa0603410

ATGGCACCAAAGGGAGAGAAGAAGCCAGCGGAGAAGAAACCCGCAGAGGAGAAGAAGTCCACCGTCGCCGAGAAAGCACCGGCGGAGAAGAAGCCAAAGGCAGGGAAGAAGCTTCCCAAGGAAGGTGGTTCTGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTTCTTAAACAAGTTCACCCGGACATTGGTATCTCAAGCAAAGCCATGGGTATCATGAACAGTTTCATCAATGACATCTTCGAGAAACTCGCTCAAGAATCATCAAGACTTGCTCGTTACAACAAGAAGCCAACAATCACTTCCAGGGAAATTCAGACTGCTGTCAGACTTGTTCTTCCCGGAGAATTGGCCAAGCATGCTGTTTCTGAGGGTACCAAAGCTGTGACCAAGTTTACTATTCTTGAAGATGTAATGAAGTCATGCAATCTGGTTGGTTCTTTTGTGTATGATAATTGTGGAATTTAA

Protein sequence

>Msa0603410

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRNKKSVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTILEDVMKSCNLVGSFVYDNCGI*