AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577280 MtrunA17_Chr8g0377891 90.831 1167 99 3 1 1161 1 1165 0.0 2098
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577280 AT3G23740.1 33.724 341 179 9 848 1162 242 561 4.09e-37 149

Find 267 sgRNAs with CRISPR-Local

Find 416 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTGAAATTTCTGAATT+AGG 0.116328 4_1:-81503202 Msa0577280:CDS
TTATTTCTCTTATGGGCTTT+GGG 0.192646 4_1:+81501882 None:intergenic
TCTTGGAAAGATGGCAATTT+TGG 0.194696 4_1:+81501118 None:intergenic
AGTGCTTTCTTGAGTGTTTC+TGG 0.194856 4_1:+81501920 None:intergenic
GGCAACTACTTCCAGGTATT+TGG 0.200105 4_1:+81498347 None:intergenic
GATCGGGAAGTAGCTGAATT+AGG 0.204752 4_1:-81503271 Msa0577280:CDS
TGAGTGGATTATTTCTCTTA+TGG 0.204932 4_1:+81501874 None:intergenic
TTGGGAAGATTCTTAAAATA+TGG 0.218302 4_1:+81501481 None:intergenic
ATTATTTCTCTTATGGGCTT+TGG 0.233144 4_1:+81501881 None:intergenic
CGACAACATCCAGGTGTTTC+AGG 0.234770 4_1:+81499687 None:intergenic
ATCCAAAAGCTTCTGAAGTT+TGG 0.240513 4_1:+81500049 None:intergenic
CTTCTGAATGGCCTAGTTAA+TGG 0.242037 4_1:-81500551 Msa0577280:CDS
CAGCTGAAATTTCTGAATTA+GGG 0.242152 4_1:-81503201 Msa0577280:CDS
GTTACATCGTTTCCACAAAA+AGG 0.243243 4_1:+81503496 None:intergenic
CCCCAAACTTCAGAAGCTTT+TGG 0.244143 4_1:-81500051 Msa0577280:CDS
TCTTCAGCATCTAACATATT+AGG 0.253871 4_1:+81500737 None:intergenic
ATCAGCTTCCCGTGCAAATA+TGG 0.256177 4_1:+81501541 None:intergenic
TGTCAACGACACTCCTCTTT+TGG 0.257812 4_1:-81499409 Msa0577280:CDS
CAACTATCAATCTTAGTTTC+AGG 0.258328 4_1:+81502329 None:intergenic
TGCAAGTTTGAATCTGAAAT+TGG 0.260828 4_1:+81500011 None:intergenic
GCTCCGTCGGTTCTGGAAAA+TGG 0.277936 4_1:-81503241 Msa0577280:CDS
GAGTGGATTATTTCTCTTAT+GGG 0.278982 4_1:+81501875 None:intergenic
AAATCTCTATGAACAGAAAT+TGG 0.285206 4_1:+81499798 None:intergenic
GATTTACCAGTCACACCTTT+AGG 0.298769 4_1:-81499780 Msa0577280:CDS
TCCAAAAGCTTCTGAAGTTT+GGG 0.301497 4_1:+81500050 None:intergenic
TGATTTAGACTCTGAGCTTC+AGG 0.305858 4_1:+81500767 None:intergenic
CGTTCCGTTTGCGGGAATTT+CGG 0.308084 4_1:+81503701 None:intergenic
GTGGTTACCATAGTCATCTT+TGG 0.316172 4_1:+81501462 None:intergenic
TCCGAACAGAAATTGTTTCT+AGG 0.321079 4_1:-81498400 Msa0577280:three_prime_UTR
CAAGCTGTCAGATGAAGATT+TGG 0.331421 4_1:-81503071 Msa0577280:CDS
TTATCTTGTAGTTGAGATCT+TGG 0.337870 4_1:+81499870 None:intergenic
AGATCGGACGTTCCGTTTGC+GGG 0.338738 4_1:+81503693 None:intergenic
TTGATCTGTGACTTTCATTT+CGG 0.339926 4_1:+81499656 None:intergenic
CAAAGCCCTCTGCATCCTCT+TGG 0.342184 4_1:+81499585 None:intergenic
GTCAAATTGTATTGGCAATT+CGG 0.344690 4_1:+81501090 None:intergenic
CAATCATTTGAATCACTTCC+AGG 0.354354 4_1:+81499981 None:intergenic
GAAATTTCTGAATTAGGGTT+TGG 0.356119 4_1:-81503196 Msa0577280:CDS
GGTGATAGTACAGGAAATTC+TGG 0.363890 4_1:-81499948 Msa0577280:CDS
AAAAGGAAGCAATAACAAAA+AGG 0.364259 4_1:-81500513 Msa0577280:CDS
CGAATTTATTCCCCACTTGC+CGG 0.365212 4_1:-81503307 Msa0577280:CDS
TCTGAGGTATGCTCTGCATT+TGG 0.369214 4_1:+81501964 None:intergenic
TCATGACAATGACCCAATTT+CGG 0.374046 4_1:+81502422 None:intergenic
TCATGAAGACAAGAAGGTTA+TGG 0.374927 4_1:-81502405 Msa0577280:CDS
ATTCAATTACAAAAGGCTAA+TGG 0.376627 4_1:-81502705 Msa0577280:CDS
AGCAAACATAAAACGAAAAG+TGG 0.377281 4_1:+81498254 None:intergenic
CAAGAAGGTTATGGGAGGTC+AGG 0.382245 4_1:-81502396 Msa0577280:CDS
ATCGGGAAGTAGCTGAATTA+GGG 0.382557 4_1:-81503270 Msa0577280:CDS
TCTTGAAACGGAACTTGAAC+CGG 0.382730 4_1:+81503547 None:intergenic
TTCCGAATCTGAAAGTCCTT+TGG 0.386134 4_1:+81503474 None:intergenic
AAGAAAGCAGTTTGTGAGTC+TGG 0.393914 4_1:-81500819 Msa0577280:CDS
TTCAGATCGTGTTGAGGAAA+AGG 0.395076 4_1:-81498477 Msa0577280:CDS
ACTTGAACCGGAACTGAGTA+TGG 0.396740 4_1:+81503559 None:intergenic
CAAAGTTGACTTGAATACTT+TGG 0.397446 4_1:-81501191 Msa0577280:CDS
TTCAGGTGATTTCTCCCTGT+TGG 0.398168 4_1:+81499704 None:intergenic
TATCTTGTAGTTGAGATCTT+GGG 0.399426 4_1:+81499871 None:intergenic
AGCTCAGAGTCTAAATCAAT+TGG 0.402575 4_1:-81500762 Msa0577280:CDS
CAAAACACCAAGCTGCTCTA+AGG 0.402756 4_1:+81502052 None:intergenic
CATGAAGACAAGAAGGTTAT+GGG 0.402943 4_1:-81502404 Msa0577280:CDS
GTACACCTGCCTTGTCCAAC+AGG 0.404686 4_1:-81499719 Msa0577280:CDS
ATACTTCCTTCATAAGGTCT+TGG 0.405275 4_1:+81499472 None:intergenic
TTTATAGGAATACCGAAATT+GGG 0.406959 4_1:-81502434 Msa0577280:intron
TACAGGTACATCTGGTGATC+AGG 0.410151 4_1:-81501332 Msa0577280:intron
CCTACATAGATGACATCATT+AGG 0.410798 4_1:+81503022 None:intergenic
AAAGCCCTCTGCATCCTCTT+GGG 0.411441 4_1:+81499586 None:intergenic
CGAAGATGACGATCTTGTTA+AGG 0.411660 4_1:-81503401 Msa0577280:CDS
TTAAGCAAGATTCTTTCAAC+AGG 0.411745 4_1:-81502118 Msa0577280:CDS
AAAGGAGTTCTCGAGATAGT+AGG 0.415123 4_1:+81499827 None:intergenic
CAAAAGGCTAATGGATGCTA+TGG 0.417412 4_1:-81502696 Msa0577280:CDS
CTCCTATAGTTGACTGAACC+AGG 0.418077 4_1:+81501511 None:intergenic
GAATCTGAAATTGGCAGAAA+TGG 0.419728 4_1:+81500020 None:intergenic
GAGTGAATCAAAGGTGGATC+TGG 0.420859 4_1:-81502195 Msa0577280:CDS
TGTTTCAGATGCTTTCCTGC+AGG 0.422230 4_1:+81498666 None:intergenic
TGGTTACCATAGTCATCTTT+GGG 0.422943 4_1:+81501463 None:intergenic
ACTTGAAACGGAACTTGAAC+CGG 0.426123 4_1:+81503603 None:intergenic
TACTTCCTTCATAAGGTCTT+GGG 0.428201 4_1:+81499473 None:intergenic
GATGCTATGGGAGATAATTT+CGG 0.429372 4_1:-81502683 Msa0577280:intron
ACAATTTGACGCAAAAGAAA+TGG 0.430175 4_1:-81501077 Msa0577280:CDS
AATCTTCCCAAAGATGACTA+TGG 0.436351 4_1:-81501469 Msa0577280:intron
CCACCATGTGTTGCAGTGTC+TGG 0.438116 4_1:+81500590 None:intergenic
TTTGCTGCGGGAACTTGAAA+CGG 0.438266 4_1:+81503591 None:intergenic
ATTTCAGTATCACGGGTCAT+TGG 0.439277 4_1:+81502014 None:intergenic
GTGTTTCTGGTCAGATCCAT+AGG 0.442538 4_1:+81501933 None:intergenic
TGTTAAGATCATCATTCATC+TGG 0.443214 4_1:+81498617 None:intergenic
TTCAAATGATTGTGATCCTA+TGG 0.444913 4_1:-81499970 Msa0577280:CDS
TTCTAGGAGTGGAACACATC+AGG 0.445762 4_1:-81498384 Msa0577280:three_prime_UTR
TGATACCAATGGGCCAGAAC+TGG 0.447913 4_1:-81502297 Msa0577280:CDS
ATGAGGCGGGTGGCGGAGAT+CGG 0.451393 4_1:+81503677 None:intergenic
TCCTAGAAACAATTTCTGTT+CGG 0.452318 4_1:+81498399 None:intergenic
TTCTCCCTGTTGGACAAGGC+AGG 0.452781 4_1:+81499714 None:intergenic
GAATCAACCATACTCAGTTC+CGG 0.453096 4_1:-81503566 Msa0577280:CDS
CCGGCGCCGGCGATTCTGAT+CGG 0.453266 4_1:-81503288 Msa0577280:CDS
AAGGTCTTGGGCAACCTCTT+CGG 0.453280 4_1:+81499485 None:intergenic
ATTGGTGAAGATGCCAGTTC+TGG 0.453428 4_1:+81502284 None:intergenic
AATGTGGATGAGGCGGGTGG+CGG 0.453864 4_1:+81503670 None:intergenic
GGTTCTCACCGGTGTAGTAT+TGG 0.455125 4_1:+81500454 None:intergenic
TATTCCCCACTTGCCGGCGC+CGG 0.457589 4_1:-81503301 Msa0577280:CDS
CGGCGCCGGCGATTCTGATC+GGG 0.457703 4_1:-81503287 Msa0577280:CDS
TCGCCTCGCACGGAGGTCCT+TGG 0.459092 4_1:+81503327 None:intergenic
TTGGCAATTCGGAGGAGTCT+TGG 0.461511 4_1:+81501101 None:intergenic
CTAACAACATGTTTCTGAGA+TGG 0.462834 4_1:+81499448 None:intergenic
GTGACAACTTGATCAGCTAT+AGG 0.464581 4_1:+81499756 None:intergenic
TTTGAGATGGCAAAGGAAGT+TGG 0.468342 4_1:+81501266 None:intergenic
CAAATGCTCTTTCTGCATGA+AGG 0.468866 4_1:+81499535 None:intergenic
GTACCCGCTCCGGTCAGATC+TGG 0.470465 4_1:-81503627 Msa0577280:CDS
GAGATCGGACGTTCCGTTTG+CGG 0.472640 4_1:+81503692 None:intergenic
CTTAACAAGATCGTCATCTT+CGG 0.472983 4_1:+81503402 None:intergenic
AGTACCAGGCAACTACTTCC+AGG 0.473034 4_1:+81498340 None:intergenic
CAGGTTCATTCAATTACAAA+AGG 0.473778 4_1:-81502712 Msa0577280:CDS
CCGATCAGAATCGCCGGCGC+CGG 0.477749 4_1:+81503288 None:intergenic
TTTCTGGGTGCTAGAGATGT+TGG 0.478007 4_1:-81502164 Msa0577280:CDS
TTGAAATTGTTCAAAGCCCC+AGG 0.478616 4_1:-81502731 Msa0577280:CDS
TAACAACATGTTTCTGAGAT+GGG 0.479696 4_1:+81499449 None:intergenic
ACCTCAGAATGTCAAACCTA+TGG 0.482063 4_1:-81501949 Msa0577280:CDS
AAAGCTCACCGTACCCGCTC+CGG 0.482065 4_1:-81503637 Msa0577280:CDS
TTCTCAGTATCTACTTGCAT+TGG 0.482411 4_1:+81502266 None:intergenic
CCTAATGATGTCATCTATGT+AGG 0.488989 4_1:-81503022 Msa0577280:CDS
TCAAACTTGCAAGTAACTCC+TGG 0.489368 4_1:-81499999 Msa0577280:CDS
TTCGGATGATAGATAATGTA+AGG 0.491626 4_1:+81498417 None:intergenic
AATCTTATCACACACCATTA+CGG 0.491836 4_1:+81500952 None:intergenic
GATGGGATACTTCCTTCATA+AGG 0.492449 4_1:+81499466 None:intergenic
CGCTCCGGTCAGATCTGGTC+CGG 0.495780 4_1:-81503622 Msa0577280:CDS
CTTACATGTTTGCTCTTGAG+TGG 0.497544 4_1:+81501858 None:intergenic
AAGCTGTCAGATGAAGATTT+GGG 0.498032 4_1:-81503070 Msa0577280:CDS
CGGGTGAATGTGGATGAGGC+GGG 0.499168 4_1:+81503664 None:intergenic
AACCAAAGGACTTTCAGATT+CGG 0.499438 4_1:-81503476 Msa0577280:CDS
AACAGGAACGGGAACGGAAG+AGG 0.499995 4_1:+81503519 None:intergenic
CGATCTGAAAACTTAACACT+TGG 0.500039 4_1:+81498491 None:intergenic
ACCTGACTTCCATCCAAAAG+AGG 0.501538 4_1:+81499396 None:intergenic
TCGGAGGAGTCTTGGAAAGA+TGG 0.504525 4_1:+81501109 None:intergenic
CTGAAAATTCAAGCGAGGGC+CGG 0.506924 4_1:-81502916 Msa0577280:CDS
TGATCAGCTATAGGACCTAA+AGG 0.507484 4_1:+81499765 None:intergenic
CCATGAGATGACATATTAGC+AGG 0.510296 4_1:+81501148 None:intergenic
TTGACATCTGATAATCATGA+AGG 0.512397 4_1:-81502359 Msa0577280:CDS
CAAATCTTCATCTGACAGCT+TGG 0.516191 4_1:+81503072 None:intergenic
GGTTCAAAGATGCCTTCAAA+AGG 0.517017 4_1:-81500530 Msa0577280:CDS
TATTTCTCTTATGGGCTTTG+GGG 0.517892 4_1:+81501883 None:intergenic
TTCAGGTGAAAGAAGCCTGC+AGG 0.518152 4_1:-81498681 Msa0577280:intron
ACTAGATTCTTCATTTGAGA+TGG 0.520142 4_1:+81500652 None:intergenic
AATCGCCGGCGCCGGCAAGT+GGG 0.521074 4_1:+81503296 None:intergenic
CCCCTGGTTCAGTCAACTAT+AGG 0.521818 4_1:-81501513 Msa0577280:CDS
TCGCCATCAATTGCTGAAAA+CGG 0.522563 4_1:-81503172 Msa0577280:CDS
GCACCATGGGTGATACCAAT+GGG 0.525467 4_1:-81502307 Msa0577280:CDS
ACAGAAATTGTTTCTAGGAG+TGG 0.525781 4_1:-81498395 Msa0577280:three_prime_UTR
GAATCGCCGGCGCCGGCAAG+TGG 0.526594 4_1:+81503295 None:intergenic
AGATCTGACCGGAGCGGGTA+CGG 0.528313 4_1:+81503629 None:intergenic
CTTGGCGTTAGTTCCCGAAA+AGG 0.530408 4_1:-81499624 Msa0577280:CDS
CCTCGCACGGAGGTCCTTGG+TGG 0.530427 4_1:+81503330 None:intergenic
AGAGCAGAGCAGAGCAGCCA+TGG 0.531954 4_1:-81503731 Msa0577280:exon
GACTGATCCTTGTTCCGTAA+TGG 0.533682 4_1:-81500966 Msa0577280:CDS
CATCATTCAAGCAATGAGAA+AGG 0.534669 4_1:-81500851 Msa0577280:CDS
TCCATAGGTTTGACATTCTG+AGG 0.534755 4_1:+81501948 None:intergenic
GAAGAGGGTTCCTCTTGAAA+CGG 0.536043 4_1:+81503535 None:intergenic
CTGAAACACCTGGATGTTGT+CGG 0.536845 4_1:-81499686 Msa0577280:CDS
AGGTTCTAAATCGCTGCCCA+TGG 0.537008 4_1:-81500287 Msa0577280:CDS
TTCATAAACCATATTTGCAC+GGG 0.537606 4_1:-81501549 Msa0577280:CDS
GGGAATAAATTCGCCTCGCA+CGG 0.538413 4_1:+81503317 None:intergenic
TGGCCCATTGGTATCACCCA+TGG 0.538471 4_1:+81502304 None:intergenic
GGACCAGATCTGACCGGAGC+GGG 0.538597 4_1:+81503624 None:intergenic
ATCACATATTTCAGTATCAC+GGG 0.541351 4_1:+81502007 None:intergenic
CCTGCTAATATGTCATCTCA+TGG 0.541971 4_1:-81501148 Msa0577280:CDS
GAAGCTTCTTCACTTTGAGA+TGG 0.542689 4_1:+81501253 None:intergenic
AGCTTTGCTGCGGGTGAATG+TGG 0.543562 4_1:+81503654 None:intergenic
ACAGATCAACTTGATGCCCT+TGG 0.544853 4_1:-81499642 Msa0577280:CDS
TGTGATGATATTGTGCTCAA+TGG 0.546109 4_1:-81501990 Msa0577280:CDS
TGATTTCTCCCTGTTGGACA+AGG 0.546492 4_1:+81499710 None:intergenic
TGATCCGAAATTCCCGCAAA+CGG 0.547137 4_1:-81503705 Msa0577280:CDS
ACAGGAACGGGAACGGAAGA+GGG 0.547351 4_1:+81503520 None:intergenic
GCGGGTGAATGTGGATGAGG+CGG 0.548554 4_1:+81503663 None:intergenic
GATCCTATGGGTGATAGTAC+AGG 0.549573 4_1:-81499957 Msa0577280:CDS
AAGATTGATAGTTGCACCAT+GGG 0.550130 4_1:-81502320 Msa0577280:CDS
TGTGATAAGATTGTCACAAA+TGG 0.552424 4_1:-81500941 Msa0577280:CDS
AAGGAGTTCTCGAGATAGTA+GGG 0.552552 4_1:+81499828 None:intergenic
TATTCAAGTCAACTTTGTCA+TGG 0.552776 4_1:+81501197 None:intergenic
AGTTGTCACTAGAGAAGAAG+TGG 0.553740 4_1:-81499742 Msa0577280:CDS
GGCTGTGCCTTAGAGCAGCT+TGG 0.560567 4_1:-81502059 Msa0577280:CDS
ATAGGACCTAAAGGTGTGAC+TGG 0.561769 4_1:+81499774 None:intergenic
GTAGATACTGAGAAAGTGAA+TGG 0.563342 4_1:-81502257 Msa0577280:CDS
GGAATTTCGGATCACGTCCA+TGG 0.563873 4_1:+81503714 None:intergenic
GCATCTTTGAACCATTAACT+AGG 0.564566 4_1:+81500540 None:intergenic
GTGTTTATCTGTGCCATCTG+TGG 0.564797 4_1:-81502231 Msa0577280:CDS
TGCACCATGGGTGATACCAA+TGG 0.565398 4_1:-81502308 Msa0577280:CDS
GACAAGCTCCGACAACATCC+AGG 0.565824 4_1:+81499678 None:intergenic
TTGATCCTCGCATCATCCAC+AGG 0.565826 4_1:+81503142 None:intergenic
AAGAAGGTTATGGGAGGTCA+GGG 0.566531 4_1:-81502395 Msa0577280:CDS
AAAAGGAACAGGAACGGGAA+CGG 0.566531 4_1:+81503513 None:intergenic
ATGATGCTCAAATGAAAGGT+TGG 0.567253 4_1:+81500868 None:intergenic
TACTTCCCGATCAGAATCGC+CGG 0.567923 4_1:+81503282 None:intergenic
TCCTACTGCTAGAAAGTACC+AGG 0.568219 4_1:+81498326 None:intergenic
TTGAATGATGCTCAAATGAA+AGG 0.568241 4_1:+81500864 None:intergenic
GTTCATAAACCATATTTGCA+CGG 0.569319 4_1:-81501550 Msa0577280:CDS
CGATGTGAGGAATGTGGAAG+AGG 0.571374 4_1:-81502984 Msa0577280:CDS
CATCACATATTTCAGTATCA+CGG 0.572878 4_1:+81502006 None:intergenic
CTTATCTGCACCCTCTGTTG+AGG 0.573087 4_1:-81501044 Msa0577280:CDS
GATAAGATTGTCACAAATGG+TGG 0.573529 4_1:-81500938 Msa0577280:CDS
AGATGCCAGTTCTGGCCCAT+TGG 0.574730 4_1:+81502292 None:intergenic
GGAAAATGGCAGTGTGAGGA+CGG 0.575753 4_1:-81503227 Msa0577280:CDS
ATCGCCGGCGCCGGCAAGTG+GGG 0.577299 4_1:+81503297 None:intergenic
AACAGAAATTGGTGAAACAA+AGG 0.578081 4_1:+81499809 None:intergenic
GTGAATGTGGATGAGGCGGG+TGG 0.579031 4_1:+81503667 None:intergenic
GGTACGGTGAGCTTTGCTGC+GGG 0.582951 4_1:+81503645 None:intergenic
TCTTCACTTTGAGATGGCAA+AGG 0.583276 4_1:+81501259 None:intergenic
GGGGCTTTGAACAATTTCAA+AGG 0.583740 4_1:+81502734 None:intergenic
GTTGGTAATGCTTTCGACCA+CGG 0.584626 4_1:-81502146 Msa0577280:CDS
AATACCTGGAAGTAGTTGCC+TGG 0.584643 4_1:-81498344 Msa0577280:three_prime_UTR
GGTTCTAAATCGCTGCCCAT+GGG 0.585890 4_1:-81500286 Msa0577280:CDS
TCACACACCATTACGGAACA+AGG 0.589266 4_1:+81500959 None:intergenic
AAAAGGCTAATGGATGCTAT+GGG 0.591299 4_1:-81502695 Msa0577280:CDS
TTTGTAATTGAATGAACCTG+GGG 0.594391 4_1:+81502715 None:intergenic
TGTGGAAACGATGTAACCAA+AGG 0.595525 4_1:-81503490 Msa0577280:CDS
TGAAACAGAACAACTTGCAA+AGG 0.595999 4_1:-81498650 Msa0577280:CDS
GGTTCAAGTTCCGTTTCAAG+AGG 0.600363 4_1:-81503545 Msa0577280:CDS
GCTGCGGGTGAATGTGGATG+AGG 0.602043 4_1:+81503660 None:intergenic
GGGAAGCTGATCAAAACCCC+TGG 0.602371 4_1:-81501529 Msa0577280:CDS
GAATAATCTTGACTGTGCTA+AGG 0.603599 4_1:-81502096 Msa0577280:CDS
CCACCAAGGACCTCCGTGCG+AGG 0.606940 4_1:-81503330 Msa0577280:CDS
GCAAACATAAAACGAAAAGT+GGG 0.607109 4_1:+81498255 None:intergenic
TCAGAGTTAGCCTCAACAGA+GGG 0.608322 4_1:+81501034 None:intergenic
TCATTGTCATGAAGACAAGA+AGG 0.608420 4_1:-81502411 Msa0577280:CDS
GACACTGCAACACATGGTGG+TGG 0.614063 4_1:-81500587 Msa0577280:CDS
GAGAAATCACCTGAAACACC+TGG 0.614071 4_1:-81499696 Msa0577280:CDS
AGGTCCTTGGTGGTAGCACA+AGG 0.615884 4_1:+81503340 None:intergenic
TTCTGGAAAATGGCAGTGTG+AGG 0.618228 4_1:-81503231 Msa0577280:CDS
GCCTGGTACTTTCTAGCAGT+AGG 0.620569 4_1:-81498327 Msa0577280:three_prime_UTR
CTGGTTCAGTCAACTATAGG+AGG 0.621061 4_1:-81501510 Msa0577280:CDS
GGGTACGGTGAGCTTTGCTG+CGG 0.621821 4_1:+81503644 None:intergenic
TGGTACTTTCTAGCAGTAGG+AGG 0.621839 4_1:-81498324 Msa0577280:three_prime_UTR
GGTTGCCCAAGACCTTATGA+AGG 0.622380 4_1:-81499478 Msa0577280:CDS
TAAATGCTTGAGTGAATCAA+AGG 0.622735 4_1:-81502204 Msa0577280:CDS
AGCAGACAAATGACTGTCAG+TGG 0.623587 4_1:-81501226 Msa0577280:CDS
ATGCTTGAGTGAATCAAAGG+TGG 0.624188 4_1:-81502201 Msa0577280:CDS
CCAAAAGCTTCTGAAGTTTG+GGG 0.630512 4_1:+81500051 None:intergenic
TCAGCTGCTAGATGCCGAAG+AGG 0.631980 4_1:-81499499 Msa0577280:CDS
GTAGCTGAAAATTCAAGCGA+GGG 0.632081 4_1:-81502920 Msa0577280:CDS
TAGAAGCTAGCTGCGATGTG+AGG 0.635076 4_1:-81502997 Msa0577280:CDS
CTCAGAGTTAGCCTCAACAG+AGG 0.638818 4_1:+81501033 None:intergenic
CCTATAGTTGACTGAACCAG+GGG 0.639145 4_1:+81501513 None:intergenic
TTTAGACTCTGAGCTTCAGG+AGG 0.640447 4_1:+81500770 None:intergenic
TGTAGCTGAAAATTCAAGCG+AGG 0.644391 4_1:-81502921 Msa0577280:CDS
AACAATTTCAAAGGTAAACG+CGG 0.645334 4_1:+81502743 None:intergenic
TTACCAGACACTGCAACACA+TGG 0.645552 4_1:-81500593 Msa0577280:CDS
TCAAATGATTGTGATCCTAT+GGG 0.646538 4_1:-81499969 Msa0577280:CDS
TTTCCTGTACTATCACCCAT+AGG 0.646734 4_1:+81499954 None:intergenic
TCATGATTATCAGATGTCAA+AGG 0.647040 4_1:+81502362 None:intergenic
TGAACCGGACCAGATCTGAC+CGG 0.648831 4_1:+81503618 None:intergenic
TAAGATTGATAGTTGCACCA+TGG 0.648864 4_1:-81502321 Msa0577280:CDS
TACACCTGCCTTGTCCAACA+GGG 0.649992 4_1:-81499718 Msa0577280:CDS
AAATTGTATTGGCAATTCGG+AGG 0.653709 4_1:+81501093 None:intergenic
AAGCATTTATCATCCACAGA+TGG 0.656457 4_1:+81502218 None:intergenic
AAAACCCAAGAGGATGCAGA+GGG 0.661927 4_1:-81499590 Msa0577280:CDS
AGAGGCGGATTTATATGCAA+AGG 0.665530 4_1:-81501293 Msa0577280:CDS
TCCTATAGTTGACTGAACCA+GGG 0.668929 4_1:+81501512 None:intergenic
GCTAAGGCTGCTGATCACAA+TGG 0.673146 4_1:-81502080 Msa0577280:CDS
ATACACCTGTGGATGATGCG+AGG 0.679193 4_1:-81503147 Msa0577280:CDS
TTTGGAATCCAATACTACAC+CGG 0.686242 4_1:-81500462 Msa0577280:intron
ATTCCCCTATCAAAATTCAG+AGG 0.686862 4_1:+81503097 None:intergenic
ATAAGATTGTCACAAATGGT+GGG 0.687693 4_1:-81500937 Msa0577280:CDS
AATAAATTCGCCTCGCACGG+AGG 0.688523 4_1:+81503320 None:intergenic
GTTGCAGTGTCTGGTAACGC+AGG 0.694475 4_1:+81500599 None:intergenic
GTTGTCACTAGAGAAGAAGT+GGG 0.694605 4_1:-81499741 Msa0577280:CDS
TCTACCTTGTGCTACCACCA+AGG 0.697929 4_1:-81503344 Msa0577280:CDS
GAAGACAAGAAGGTTATGGG+AGG 0.705451 4_1:-81502401 Msa0577280:CDS
ACATAGATGACATCATTAGG+TGG 0.709990 4_1:+81503025 None:intergenic
CCAGACACTGCAACACATGG+TGG 0.710994 4_1:-81500590 Msa0577280:CDS
TGAAAACGGCGATACACCTG+TGG 0.720243 4_1:-81503158 Msa0577280:CDS
CGGACCAGATCTGACCGGAG+CGG 0.748187 4_1:+81503623 None:intergenic
TAGCTGCGATGTGAGGAATG+TGG 0.763594 4_1:-81502990 Msa0577280:CDS
AATCTTGCTTAACATCACCG+TGG 0.800564 4_1:+81502129 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATAATATTA+TGG - chr4_1:81500533-81500552 Msa0577280:CDS 0.0%
!!! TTATTTTTCATTTTTTTGAT+TGG - chr4_1:81501779-81501798 Msa0577280:intron 10.0%
!!! ATAAGTTTAGTTTTAAAAAA+CGG + chr4_1:81501807-81501826 None:intergenic 10.0%
!!! TTTTTTATTTGAATATTTTC+AGG - chr4_1:81503213-81503232 Msa0577280:CDS 10.0%
!! AAATAAACAAATTGTTTATT+TGG + chr4_1:81503371-81503390 None:intergenic 10.0%
!! TAAAAAAACAAATTTCTATA+AGG + chr4_1:81503634-81503653 None:intergenic 10.0%
!! TGATGAAAATAAAAATTGAA+AGG + chr4_1:81499057-81499076 None:intergenic 15.0%
!! ATTCAATCAATAAATAACAA+AGG + chr4_1:81499395-81499414 None:intergenic 15.0%
!! ATTTAAATAACCAAACTTAT+CGG + chr4_1:81502716-81502735 None:intergenic 15.0%
!!! TGATATAAATTTATTTTCTC+AGG - chr4_1:81502861-81502880 Msa0577280:intron 15.0%
!! ATAGTGAAAAATAAATACTA+GGG + chr4_1:81503715-81503734 None:intergenic 15.0%
!! AATAGTGAAAAATAAATACT+AGG + chr4_1:81503716-81503735 None:intergenic 15.0%
!!! ATGAAATTTTGTTAGTTGAT+TGG - chr4_1:81499123-81499142 Msa0577280:intron 20.0%
!! TGCAAACAACTAAATAATAA+AGG + chr4_1:81499275-81499294 None:intergenic 20.0%
!! AATTATATATCATGTAACCT+GGG - chr4_1:81500326-81500345 Msa0577280:intron 20.0%
!! TTAATATTGTATTGCATAAG+TGG + chr4_1:81500486-81500505 None:intergenic 20.0%
!!! TTAAGCAAATTTTGTTCTTT+GGG + chr4_1:81501006-81501025 None:intergenic 20.0%
!!! ATTAAGCAAATTTTGTTCTT+TGG + chr4_1:81501007-81501026 None:intergenic 20.0%
!! GATATATACACAAAAGATTA+TGG + chr4_1:81501481-81501500 None:intergenic 20.0%
!!! TTAAGTTTTTAAAACATGCT+GGG - chr4_1:81501648-81501667 Msa0577280:intron 20.0%
!!! AGTATTGGTTTTTATTCTAT+TGG - chr4_1:81501732-81501751 Msa0577280:intron 20.0%
!!! ACTAAACTTATACTACTTTT+AGG - chr4_1:81501816-81501835 Msa0577280:intron 20.0%
!!! TTTTTCTCAAAATTCCTTTT+CGG + chr4_1:81502304-81502323 None:intergenic 20.0%
!! ATAAAATGATCAGAAAAGAA+AGG + chr4_1:81502587-81502606 None:intergenic 20.0%
!! TGAAATGAAATAATAGTGAA+AGG + chr4_1:81502757-81502776 None:intergenic 20.0%
!! GCAAAATATTATAAACTTGT+CGG + chr4_1:81502915-81502934 None:intergenic 20.0%
!! TAAACAAATTGTTTATTTGG+AGG + chr4_1:81503368-81503387 None:intergenic 20.0%
!!! TAACCATTTTTTAATCTGTT+TGG + chr4_1:81503468-81503487 None:intergenic 20.0%
!! TTTTGTAATTGAATGAACCT+GGG + chr4_1:81499200-81499219 None:intergenic 25.0%
! ATTCAATTACAAAAGGCTAA+TGG - chr4_1:81499206-81499225 Msa0577280:intron 25.0%
!! AATTGGCATCGTAATTTTAT+AGG - chr4_1:81499462-81499481 Msa0577280:CDS 25.0%
!! TTTTATAGGAATACCGAAAT+TGG - chr4_1:81499476-81499495 Msa0577280:CDS 25.0%
! TTTATAGGAATACCGAAATT+GGG - chr4_1:81499477-81499496 Msa0577280:CDS 25.0%
! TTGACATTGTATTAGGTATT+AGG + chr4_1:81500166-81500185 None:intergenic 25.0%
! TACCTAATACAATGTCAATT+TGG - chr4_1:81500168-81500187 Msa0577280:intron 25.0%
! AACCAAATTGACATTGTATT+AGG + chr4_1:81500173-81500192 None:intergenic 25.0%
! TGACAATATATGCATATACA+TGG - chr4_1:81500287-81500306 Msa0577280:CDS 25.0%
!!! ATACATGGTTTTGTATTTGT+TGG - chr4_1:81500302-81500321 Msa0577280:intron 25.0%
! CAATTATATATCATGTAACC+TGG - chr4_1:81500325-81500344 Msa0577280:intron 25.0%
! TTGGGAAGATTCTTAAAATA+TGG + chr4_1:81500433-81500452 None:intergenic 25.0%
! AAAGAAAAAAGTCTTCTGAA+TGG - chr4_1:81501348-81501367 Msa0577280:intron 25.0%
! AAAAGGAAGCAATAACAAAA+AGG - chr4_1:81501398-81501417 Msa0577280:intron 25.0%
! TATAATCAATGAGAAAGAGA+GGG + chr4_1:81501535-81501554 None:intergenic 25.0%
! TTATAATCAATGAGAAAGAG+AGG + chr4_1:81501536-81501555 None:intergenic 25.0%
!! TTGATCAAGTAATTTTGAAC+AGG - chr4_1:81501604-81501623 Msa0577280:intron 25.0%
!!! GTTTTAAAAACTTAACCCAT+GGG + chr4_1:81501643-81501662 None:intergenic 25.0%
!!! TGTTTTAAAAACTTAACCCA+TGG + chr4_1:81501644-81501663 None:intergenic 25.0%
!!! GTTAAGTTTTTAAAACATGC+TGG - chr4_1:81501647-81501666 Msa0577280:intron 25.0%
!! TTCTCATTTTCCAACAAAAT+CGG + chr4_1:81501698-81501717 None:intergenic 25.0%
! GAATAAAAACCAATACTTAC+AGG + chr4_1:81501729-81501748 None:intergenic 25.0%
! AAATCTCTATGAACAGAAAT+TGG + chr4_1:81502116-81502135 None:intergenic 25.0%
!! TTTTCTCAAAATTCCTTTTC+GGG + chr4_1:81502303-81502322 None:intergenic 25.0%
!!! CTTGAGGTTTTATTTCTAAA+TGG + chr4_1:81502944-81502963 None:intergenic 25.0%
! ATAAAACCTCAAGTAAATTC+TGG - chr4_1:81502951-81502970 Msa0577280:CDS 25.0%
! AAAAAAATGGCAAAGTTACT+TGG + chr4_1:81503007-81503026 None:intergenic 25.0%
! CTGAATTTATTAAGTTACAC+GGG + chr4_1:81503113-81503132 None:intergenic 25.0%
! GCTGAATTTATTAAGTTACA+CGG + chr4_1:81503114-81503133 None:intergenic 25.0%
!! TATTTGGAGGAACAAATTTT+GGG + chr4_1:81503355-81503374 None:intergenic 25.0%
!! TTATTTGGAGGAACAAATTT+TGG + chr4_1:81503356-81503375 None:intergenic 25.0%
! CAACCAAACAGATTAAAAAA+TGG - chr4_1:81503462-81503481 Msa0577280:CDS 25.0%
!! TGCTGTATATACTCTTTTTA+CGG - chr4_1:81503676-81503695 Msa0577280:CDS 25.0%
TCAGCTGAAATTTCTGAATT+AGG - chr4_1:81498709-81498728 Msa0577280:intron 30.0%
CAGCTGAAATTTCTGAATTA+GGG - chr4_1:81498710-81498729 Msa0577280:intron 30.0%
GAAATTTCTGAATTAGGGTT+TGG - chr4_1:81498715-81498734 Msa0577280:intron 30.0%
!!! TTTGAACAGAGCTATTTTTC+CGG + chr4_1:81499017-81499036 None:intergenic 30.0%
TTCAAAGGAGTAAATCTGTA+AGG - chr4_1:81499031-81499050 Msa0577280:intron 30.0%
TTTCATCATTGCATATGTGT+TGG - chr4_1:81499069-81499088 Msa0577280:intron 30.0%
AACAATTTCAAAGGTAAACG+CGG + chr4_1:81499171-81499190 None:intergenic 30.0%
TTTGTAATTGAATGAACCTG+GGG + chr4_1:81499199-81499218 None:intergenic 30.0%
! CTTTTGTAATTGAATGAACC+TGG + chr4_1:81499201-81499220 None:intergenic 30.0%
CAGGTTCATTCAATTACAAA+AGG - chr4_1:81499199-81499218 Msa0577280:intron 30.0%
!!! ATTTAGTTTTACATGTGCGA+CGG - chr4_1:81499309-81499328 Msa0577280:intron 30.0%
! GATACTATTGTACGTGATTT+TGG - chr4_1:81499348-81499367 Msa0577280:intron 30.0%
TCATGATTATCAGATGTCAA+AGG + chr4_1:81499552-81499571 None:intergenic 30.0%
TTGACATCTGATAATCATGA+AGG - chr4_1:81499552-81499571 Msa0577280:CDS 30.0%
CAACTATCAATCTTAGTTTC+AGG + chr4_1:81499585-81499604 None:intergenic 30.0%
TAAATGCTTGAGTGAATCAA+AGG - chr4_1:81499707-81499726 Msa0577280:CDS 30.0%
TTAAGCAAGATTCTTTCAAC+AGG - chr4_1:81499793-81499812 Msa0577280:CDS 30.0%
ATCACATATTTCAGTATCAC+GGG + chr4_1:81499907-81499926 None:intergenic 30.0%
CATCACATATTTCAGTATCA+CGG + chr4_1:81499908-81499927 None:intergenic 30.0%
! TTATTTCTCTTATGGGCTTT+GGG + chr4_1:81500032-81500051 None:intergenic 30.0%
! ATTATTTCTCTTATGGGCTT+TGG + chr4_1:81500033-81500052 None:intergenic 30.0%
!! GAGTGGATTATTTCTCTTAT+GGG + chr4_1:81500039-81500058 None:intergenic 30.0%
!! TGAGTGGATTATTTCTCTTA+TGG + chr4_1:81500040-81500059 None:intergenic 30.0%
!! TATTTGTTTTCTGCCAGTTA+TGG - chr4_1:81500095-81500114 Msa0577280:intron 30.0%
!! ATTTGTTTTCTGCCAGTTAT+GGG - chr4_1:81500096-81500115 Msa0577280:intron 30.0%
TGTTTAAACTTCCACTATAG+TGG - chr4_1:81500229-81500248 Msa0577280:CDS 30.0%
GTTCATAAACCATATTTGCA+CGG - chr4_1:81500361-81500380 Msa0577280:intron 30.0%
TTCATAAACCATATTTGCAC+GGG - chr4_1:81500362-81500381 Msa0577280:intron 30.0%
TATTCAAGTCAACTTTGTCA+TGG + chr4_1:81500717-81500736 None:intergenic 30.0%
!! CAAAGTTGACTTGAATACTT+TGG - chr4_1:81500720-81500739 Msa0577280:CDS 30.0%
! GTCAAATTGTATTGGCAATT+CGG + chr4_1:81500824-81500843 None:intergenic 30.0%
! TCTTTTGCGTCAAATTGTAT+TGG + chr4_1:81500832-81500851 None:intergenic 30.0%
ACAATTTGACGCAAAAGAAA+TGG - chr4_1:81500834-81500853 Msa0577280:CDS 30.0%
AATCTTATCACACACCATTA+CGG + chr4_1:81500962-81500981 None:intergenic 30.0%
TGTGATAAGATTGTCACAAA+TGG - chr4_1:81500970-81500989 Msa0577280:CDS 30.0%
ATAAGATTGTCACAAATGGT+GGG - chr4_1:81500974-81500993 Msa0577280:CDS 30.0%
TTGAATGATGCTCAAATGAA+AGG + chr4_1:81501050-81501069 None:intergenic 30.0%
TCTTCAGCATCTAACATATT+AGG + chr4_1:81501177-81501196 None:intergenic 30.0%
ACTAGATTCTTCATTTGAGA+TGG + chr4_1:81501262-81501281 None:intergenic 30.0%
!! TTGTTATTGCTTCCTTTTGA+AGG + chr4_1:81501396-81501415 None:intergenic 30.0%
!! TTAAAAGTATGCAGGTCTTT+TGG + chr4_1:81501505-81501524 None:intergenic 30.0%
! GGTAAATCTTAAAAGTATGC+AGG + chr4_1:81501513-81501532 None:intergenic 30.0%
ATAATCAATGAGAAAGAGAG+GGG + chr4_1:81501534-81501553 None:intergenic 30.0%
AGTCTAATTCCTGTAAGTAT+TGG - chr4_1:81501717-81501736 Msa0577280:intron 30.0%
! TGCAAGTTTGAATCTGAAAT+TGG + chr4_1:81501903-81501922 None:intergenic 30.0%
TTCAAATGATTGTGATCCTA+TGG - chr4_1:81501941-81501960 Msa0577280:CDS 30.0%
TCAAATGATTGTGATCCTAT+GGG - chr4_1:81501942-81501961 Msa0577280:CDS 30.0%
TATCTTGTAGTTGAGATCTT+GGG + chr4_1:81502043-81502062 None:intergenic 30.0%
TTATCTTGTAGTTGAGATCT+TGG + chr4_1:81502044-81502063 None:intergenic 30.0%
AACAGAAATTGGTGAAACAA+AGG + chr4_1:81502105-81502124 None:intergenic 30.0%
TTGATCTGTGACTTTCATTT+CGG + chr4_1:81502258-81502277 None:intergenic 30.0%
!! ATTTTGAGAAAAAACCCAAG+AGG - chr4_1:81502311-81502330 Msa0577280:CDS 30.0%
!! TAACAACATGTTTCTGAGAT+GGG + chr4_1:81502465-81502484 None:intergenic 30.0%
ATAGTCATATCAACATCATG+TGG + chr4_1:81502546-81502565 None:intergenic 30.0%
CTTCACGATAATAACTGAAA+TGG - chr4_1:81502611-81502630 Msa0577280:intron 30.0%
TTATTATGCAAAGCTTGTGA+TGG - chr4_1:81502666-81502685 Msa0577280:intron 30.0%
ATTATCTCATCCGATAAGTT+TGG - chr4_1:81502703-81502722 Msa0577280:CDS 30.0%
TAATAGTGAAAGGAATTGCA+AGG + chr4_1:81502747-81502766 None:intergenic 30.0%
GGTCAAATGCACTAAAAAAA+TGG + chr4_1:81503020-81503039 None:intergenic 30.0%
TCAAATGAAGCTATCTCATA+AGG + chr4_1:81503041-81503060 None:intergenic 30.0%
! GCATATAAAGACGTTGATAA+TGG + chr4_1:81503136-81503155 None:intergenic 30.0%
TGTTAAGATCATCATTCATC+TGG + chr4_1:81503297-81503316 None:intergenic 30.0%
!! AGGAACAAATTTTGGGAATA+AGG + chr4_1:81503348-81503367 None:intergenic 30.0%
TTCGGATGATAGATAATGTA+AGG + chr4_1:81503497-81503516 None:intergenic 30.0%
TCCTAGAAACAATTTCTGTT+CGG + chr4_1:81503515-81503534 None:intergenic 30.0%
!!! GTGTTTTAAAACCAAATACC+TGG - chr4_1:81503553-81503572 Msa0577280:CDS 30.0%
GCAAACATAAAACGAAAAGT+GGG + chr4_1:81503659-81503678 None:intergenic 30.0%
AGCAAACATAAAACGAAAAG+TGG + chr4_1:81503660-81503679 None:intergenic 30.0%
!! ATGGTTGATTCTTTTTGCTG+CGG + chr4_1:81498336-81498355 None:intergenic 35.0%
GTTACATCGTTTCCACAAAA+AGG + chr4_1:81498418-81498437 None:intergenic 35.0%
AACCAAAGGACTTTCAGATT+CGG - chr4_1:81498435-81498454 Msa0577280:exon 35.0%
CTTAACAAGATCGTCATCTT+CGG + chr4_1:81498512-81498531 None:intergenic 35.0%
! TCACGTTTTGCTTTCTTGAA+AGG + chr4_1:81498538-81498557 None:intergenic 35.0%
ATTCCCCTATCAAAATTCAG+AGG + chr4_1:81498817-81498836 None:intergenic 35.0%
AAGCTGTCAGATGAAGATTT+GGG - chr4_1:81498841-81498860 Msa0577280:intron 35.0%
ACATAGATGACATCATTAGG+TGG + chr4_1:81498889-81498908 None:intergenic 35.0%
CCTACATAGATGACATCATT+AGG + chr4_1:81498892-81498911 None:intergenic 35.0%
CCTAATGATGTCATCTATGT+AGG - chr4_1:81498889-81498908 Msa0577280:intron 35.0%
! GGAAAAATAGCTCTGTTCAA+AGG - chr4_1:81499016-81499035 Msa0577280:intron 35.0%
! AAAAGGCTAATGGATGCTAT+GGG - chr4_1:81499216-81499235 Msa0577280:intron 35.0%
GATGCTATGGGAGATAATTT+CGG - chr4_1:81499228-81499247 Msa0577280:intron 35.0%
! TTGGTAGTTGTTCTATGTAG+AGG - chr4_1:81499367-81499386 Msa0577280:intron 35.0%
! AATTAGCTTGATATGTGCGA+TGG - chr4_1:81499435-81499454 Msa0577280:CDS 35.0%
ATATGTGCGATGGAACTAAT+TGG - chr4_1:81499445-81499464 Msa0577280:CDS 35.0%
TCATGACAATGACCCAATTT+CGG + chr4_1:81499492-81499511 None:intergenic 35.0%
TCATTGTCATGAAGACAAGA+AGG - chr4_1:81499500-81499519 Msa0577280:CDS 35.0%
TCATGAAGACAAGAAGGTTA+TGG - chr4_1:81499506-81499525 Msa0577280:CDS 35.0%
CATGAAGACAAGAAGGTTAT+GGG - chr4_1:81499507-81499526 Msa0577280:CDS 35.0%
! TAAGATTGATAGTTGCACCA+TGG - chr4_1:81499590-81499609 Msa0577280:CDS 35.0%
! AAGATTGATAGTTGCACCAT+GGG - chr4_1:81499591-81499610 Msa0577280:CDS 35.0%
TTCTCAGTATCTACTTGCAT+TGG + chr4_1:81499648-81499667 None:intergenic 35.0%
GTAGATACTGAGAAAGTGAA+TGG - chr4_1:81499654-81499673 Msa0577280:CDS 35.0%
AAGCATTTATCATCCACAGA+TGG + chr4_1:81499696-81499715 None:intergenic 35.0%
GAATAATCTTGACTGTGCTA+AGG - chr4_1:81499815-81499834 Msa0577280:CDS 35.0%
TGTGATGATATTGTGCTCAA+TGG - chr4_1:81499921-81499940 Msa0577280:CDS 35.0%
TATTTCTCTTATGGGCTTTG+GGG + chr4_1:81500031-81500050 None:intergenic 35.0%
CAAAATGTCTAACCCATAAC+TGG + chr4_1:81500111-81500130 None:intergenic 35.0%
AATCTTCCCAAAGATGACTA+TGG - chr4_1:81500442-81500461 Msa0577280:intron 35.0%
TGGTTACCATAGTCATCTTT+GGG + chr4_1:81500451-81500470 None:intergenic 35.0%
!!! AAAGTGCTTGCTGATTTTAC+AGG - chr4_1:81500562-81500581 Msa0577280:CDS 35.0%
! TCTTGGAAAGATGGCAATTT+TGG + chr4_1:81500796-81500815 None:intergenic 35.0%
! AAATTGTATTGGCAATTCGG+AGG + chr4_1:81500821-81500840 None:intergenic 35.0%
GATAAGATTGTCACAAATGG+TGG - chr4_1:81500973-81500992 Msa0577280:CDS 35.0%
! AGCAAATTTTGTTCTTTGGG+TGG + chr4_1:81501003-81501022 None:intergenic 35.0%
ATGATGCTCAAATGAAAGGT+TGG + chr4_1:81501046-81501065 None:intergenic 35.0%
CATCATTCAAGCAATGAGAA+AGG - chr4_1:81501060-81501079 Msa0577280:CDS 35.0%
AGCTCAGAGTCTAAATCAAT+TGG - chr4_1:81501149-81501168 Msa0577280:CDS 35.0%
!!! CGTCATTTTTGCAAATTGCA+TGG + chr4_1:81501215-81501234 None:intergenic 35.0%
TTGCAAAAATGACGATGTTG+TGG - chr4_1:81501221-81501240 Msa0577280:CDS 35.0%
GCATCTTTGAACCATTAACT+AGG + chr4_1:81501374-81501393 None:intergenic 35.0%
!!! AACAAAAAGGCTAGTTTTGC+TGG - chr4_1:81501411-81501430 Msa0577280:intron 35.0%
TTTGCTGGTGAGATGAAAAA+TGG - chr4_1:81501426-81501445 Msa0577280:intron 35.0%
TGGTGAGATGAAAAATGGTT+TGG - chr4_1:81501431-81501450 Msa0577280:intron 35.0%
! TTTGGAATCCAATACTACAC+CGG - chr4_1:81501449-81501468 Msa0577280:intron 35.0%
CAAAAGATTATGGTTCTCAC+CGG + chr4_1:81501471-81501490 None:intergenic 35.0%
! AAGATTGCTTCCGATTTTGT+TGG - chr4_1:81501685-81501704 Msa0577280:intron 35.0%
!! TCCAAAAGCTTCTGAAGTTT+GGG + chr4_1:81501864-81501883 None:intergenic 35.0%
!! ATCCAAAAGCTTCTGAAGTT+TGG + chr4_1:81501865-81501884 None:intergenic 35.0%
! GAATCTGAAATTGGCAGAAA+TGG + chr4_1:81501894-81501913 None:intergenic 35.0%
CAATCATTTGAATCACTTCC+AGG + chr4_1:81501933-81501952 None:intergenic 35.0%
TACTTCCTTCATAAGGTCTT+GGG + chr4_1:81502441-81502460 None:intergenic 35.0%
ATACTTCCTTCATAAGGTCT+TGG + chr4_1:81502442-81502461 None:intergenic 35.0%
! CTAACAACATGTTTCTGAGA+TGG + chr4_1:81502466-81502485 None:intergenic 35.0%
ACTTGGAATTTCTCACCATA+GGG + chr4_1:81502990-81503009 None:intergenic 35.0%
TACTTGGAATTTCTCACCAT+AGG + chr4_1:81502991-81503010 None:intergenic 35.0%
TGAAACAGAACAACTTGCAA+AGG - chr4_1:81503261-81503280 Msa0577280:CDS 35.0%
CGATCTGAAAACTTAACACT+TGG + chr4_1:81503423-81503442 None:intergenic 35.0%
! TAAGTTTTCAGATCGTGTTG+AGG - chr4_1:81503428-81503447 Msa0577280:CDS 35.0%
! TCCGAACAGAAATTGTTTCT+AGG - chr4_1:81503511-81503530 Msa0577280:CDS 35.0%
!! ACAGAAATTGTTTCTAGGAG+TGG - chr4_1:81503516-81503535 Msa0577280:CDS 35.0%
ACTTGAAACGGAACTTGAAC+CGG + chr4_1:81498311-81498330 None:intergenic 40.0%
!! TGGTTGATTCTTTTTGCTGC+GGG + chr4_1:81498335-81498354 None:intergenic 40.0%
GAATCAACCATACTCAGTTC+CGG - chr4_1:81498345-81498364 Msa0577280:three_prime_UTR 40.0%
TCTTGAAACGGAACTTGAAC+CGG + chr4_1:81498367-81498386 None:intergenic 40.0%
TCCACAAAAAGGAACAGGAA+CGG + chr4_1:81498407-81498426 None:intergenic 40.0%
TCGTTTCCACAAAAAGGAAC+AGG + chr4_1:81498412-81498431 None:intergenic 40.0%
TGTGGAAACGATGTAACCAA+AGG - chr4_1:81498421-81498440 Msa0577280:three_prime_UTR 40.0%
TTCCGAATCTGAAAGTCCTT+TGG + chr4_1:81498440-81498459 None:intergenic 40.0%
CGAAGATGACGATCTTGTTA+AGG - chr4_1:81498510-81498529 Msa0577280:intron 40.0%
ATCGGGAAGTAGCTGAATTA+GGG - chr4_1:81498641-81498660 Msa0577280:CDS 40.0%
!! ATTAGGGTTTTATGCTCCGT+CGG - chr4_1:81498657-81498676 Msa0577280:CDS 40.0%
TCGCCATCAATTGCTGAAAA+CGG - chr4_1:81498739-81498758 Msa0577280:intron 40.0%
!! ACGAGCCTCTGAATTTTGAT+AGG - chr4_1:81498809-81498828 Msa0577280:intron 40.0%
!! CGAGCCTCTGAATTTTGATA+GGG - chr4_1:81498810-81498829 Msa0577280:intron 40.0%
!! GAGCCTCTGAATTTTGATAG+GGG - chr4_1:81498811-81498830 Msa0577280:intron 40.0%
CAAATCTTCATCTGACAGCT+TGG + chr4_1:81498842-81498861 None:intergenic 40.0%
CAAGCTGTCAGATGAAGATT+TGG - chr4_1:81498840-81498859 Msa0577280:intron 40.0%
! TTTTCAGCTACATTAGCAGC+AGG + chr4_1:81498982-81499001 None:intergenic 40.0%
TGTAGCTGAAAATTCAAGCG+AGG - chr4_1:81498990-81499009 Msa0577280:intron 40.0%
GTAGCTGAAAATTCAAGCGA+GGG - chr4_1:81498991-81499010 Msa0577280:intron 40.0%
GTTGATTGGATTCTTGATGC+AGG - chr4_1:81499137-81499156 Msa0577280:intron 40.0%
!! GGGGCTTTGAACAATTTCAA+AGG + chr4_1:81499180-81499199 None:intergenic 40.0%
TTGAAATTGTTCAAAGCCCC+AGG - chr4_1:81499180-81499199 Msa0577280:intron 40.0%
! CAAAAGGCTAATGGATGCTA+TGG - chr4_1:81499215-81499234 Msa0577280:intron 40.0%
ATGCTTGAGTGAATCAAAGG+TGG - chr4_1:81499710-81499729 Msa0577280:CDS 40.0%
AATCTTGCTTAACATCACCG+TGG + chr4_1:81499785-81499804 None:intergenic 40.0%
ATTTCAGTATCACGGGTCAT+TGG + chr4_1:81499900-81499919 None:intergenic 40.0%
ACCTCAGAATGTCAAACCTA+TGG - chr4_1:81499962-81499981 Msa0577280:CDS 40.0%
TCCATAGGTTTGACATTCTG+AGG + chr4_1:81499966-81499985 None:intergenic 40.0%
AGTGCTTTCTTGAGTGTTTC+TGG + chr4_1:81499994-81500013 None:intergenic 40.0%
CTTACATGTTTGCTCTTGAG+TGG + chr4_1:81500056-81500075 None:intergenic 40.0%
AATTTGGTTAAACCCTCAGC+TGG - chr4_1:81500184-81500203 Msa0577280:intron 40.0%
ATTTGGTTAAACCCTCAGCT+GGG - chr4_1:81500185-81500204 Msa0577280:intron 40.0%
CCTAAAAAAAACCCAGCTGA+GGG + chr4_1:81500199-81500218 None:intergenic 40.0%
!!! CCTCAGCTGGGTTTTTTTTA+GGG - chr4_1:81500197-81500216 Msa0577280:intron 40.0%
TAAACTTCCACTATAGTGGC+TGG - chr4_1:81500233-81500252 Msa0577280:CDS 40.0%
TCCTATAGTTGACTGAACCA+GGG + chr4_1:81500402-81500421 None:intergenic 40.0%
GTGGTTACCATAGTCATCTT+TGG + chr4_1:81500452-81500471 None:intergenic 40.0%
ATAAGTGGTTGAAAACGTGG+TGG + chr4_1:81500471-81500490 None:intergenic 40.0%
TGCATAAGTGGTTGAAAACG+TGG + chr4_1:81500474-81500493 None:intergenic 40.0%
!! GCTGATTTTACAGGTACATC+TGG - chr4_1:81500571-81500590 Msa0577280:CDS 40.0%
!! CGCCTCTTCATTTTTGTGAT+GGG + chr4_1:81500605-81500624 None:intergenic 40.0%
CCATCACAAAAATGAAGAGG+CGG - chr4_1:81500603-81500622 Msa0577280:CDS 40.0%
! AGAGGCGGATTTATATGCAA+AGG - chr4_1:81500618-81500637 Msa0577280:CDS 40.0%
! TTTGAGATGGCAAAGGAAGT+TGG + chr4_1:81500648-81500667 None:intergenic 40.0%
!! TCTTCACTTTGAGATGGCAA+AGG + chr4_1:81500655-81500674 None:intergenic 40.0%
! GAAGCTTCTTCACTTTGAGA+TGG + chr4_1:81500661-81500680 None:intergenic 40.0%
CCATGAGATGACATATTAGC+AGG + chr4_1:81500766-81500785 None:intergenic 40.0%
CCTGCTAATATGTCATCTCA+TGG - chr4_1:81500763-81500782 Msa0577280:CDS 40.0%
AAGAAAGCAGTTTGTGAGTC+TGG - chr4_1:81501092-81501111 Msa0577280:CDS 40.0%
TGATTTAGACTCTGAGCTTC+AGG + chr4_1:81501147-81501166 None:intergenic 40.0%
CTTCTGAATGGCCTAGTTAA+TGG - chr4_1:81501360-81501379 Msa0577280:intron 40.0%
GGTTCAAAGATGCCTTCAAA+AGG - chr4_1:81501381-81501400 Msa0577280:intron 40.0%
!! CCAAAAGCTTCTGAAGTTTG+GGG + chr4_1:81501863-81501882 None:intergenic 40.0%
TCAAACTTGCAAGTAACTCC+TGG - chr4_1:81501912-81501931 Msa0577280:CDS 40.0%
TTTCCTGTACTATCACCCAT+AGG + chr4_1:81501960-81501979 None:intergenic 40.0%
GGTGATAGTACAGGAAATTC+TGG - chr4_1:81501963-81501982 Msa0577280:CDS 40.0%
AAGGAGTTCTCGAGATAGTA+GGG + chr4_1:81502086-81502105 None:intergenic 40.0%
AAAGGAGTTCTCGAGATAGT+AGG + chr4_1:81502087-81502106 None:intergenic 40.0%
GATTTACCAGTCACACCTTT+AGG - chr4_1:81502131-81502150 Msa0577280:CDS 40.0%
TGATCAGCTATAGGACCTAA+AGG + chr4_1:81502149-81502168 None:intergenic 40.0%
GTGACAACTTGATCAGCTAT+AGG + chr4_1:81502158-81502177 None:intergenic 40.0%
AGTTGTCACTAGAGAAGAAG+TGG - chr4_1:81502169-81502188 Msa0577280:CDS 40.0%
GTTGTCACTAGAGAAGAAGT+GGG - chr4_1:81502170-81502189 Msa0577280:CDS 40.0%
CAAATGCTCTTTCTGCATGA+AGG + chr4_1:81502379-81502398 None:intergenic 40.0%
GATGGGATACTTCCTTCATA+AGG + chr4_1:81502448-81502467 None:intergenic 40.0%
ATAGGGCCAGAATTTACTTG+AGG + chr4_1:81502960-81502979 None:intergenic 40.0%
TTGGAATTTCTCACCATAGG+GGG + chr4_1:81502988-81503007 None:intergenic 40.0%
CTTGGAATTTCTCACCATAG+GGG + chr4_1:81502989-81503008 None:intergenic 40.0%
GGAATAAGGTATGCACATAC+AGG + chr4_1:81503334-81503353 None:intergenic 40.0%
TTCAGATCGTGTTGAGGAAA+AGG - chr4_1:81503434-81503453 Msa0577280:CDS 40.0%
GCAGTAGGAGGAAAAATAGA+TGG - chr4_1:81503599-81503618 Msa0577280:CDS 40.0%
TGATCCGAAATTCCCGCAAA+CGG - chr4_1:81498206-81498225 Msa0577280:three_prime_UTR 45.0%
TTTGCTGCGGGAACTTGAAA+CGG + chr4_1:81498323-81498342 None:intergenic 45.0%
ACTTGAACCGGAACTGAGTA+TGG + chr4_1:81498355-81498374 None:intergenic 45.0%
! GGTTCAAGTTCCGTTTCAAG+AGG - chr4_1:81498310-81498329 Msa0577280:three_prime_UTR 45.0%
GAAGAGGGTTCCTCTTGAAA+CGG + chr4_1:81498379-81498398 None:intergenic 45.0%
AAAAGGAACAGGAACGGGAA+CGG + chr4_1:81498401-81498420 None:intergenic 45.0%
CCACAAAAAGGAACAGGAAC+GGG + chr4_1:81498406-81498425 None:intergenic 45.0%
CGAATTTATTCCCCACTTGC+CGG - chr4_1:81498604-81498623 Msa0577280:CDS 45.0%
GATCGGGAAGTAGCTGAATT+AGG - chr4_1:81498640-81498659 Msa0577280:CDS 45.0%
! TTCTGGAAAATGGCAGTGTG+AGG - chr4_1:81498680-81498699 Msa0577280:intron 45.0%
!! TCGCCGTTTTCAGCAATTGA+TGG + chr4_1:81498745-81498764 None:intergenic 45.0%
GAAGACAAGAAGGTTATGGG+AGG - chr4_1:81499510-81499529 Msa0577280:CDS 45.0%
AAGAAGGTTATGGGAGGTCA+GGG - chr4_1:81499516-81499535 Msa0577280:CDS 45.0%
ATTGGTGAAGATGCCAGTTC+TGG + chr4_1:81499630-81499649 None:intergenic 45.0%
GTGTTTATCTGTGCCATCTG+TGG - chr4_1:81499680-81499699 Msa0577280:CDS 45.0%
GAGTGAATCAAAGGTGGATC+TGG - chr4_1:81499716-81499735 Msa0577280:CDS 45.0%
! GATCTGGCTGCAGATTTTCT+GGG - chr4_1:81499732-81499751 Msa0577280:CDS 45.0%
TTTCTGGGTGCTAGAGATGT+TGG - chr4_1:81499747-81499766 Msa0577280:CDS 45.0%
!! GTTGGTAATGCTTTCGACCA+CGG - chr4_1:81499765-81499784 Msa0577280:CDS 45.0%
CAAAACACCAAGCTGCTCTA+AGG + chr4_1:81499862-81499881 None:intergenic 45.0%
! TCTGAGGTATGCTCTGCATT+TGG + chr4_1:81499950-81499969 None:intergenic 45.0%
!! GTGTTTCTGGTCAGATCCAT+AGG + chr4_1:81499981-81500000 None:intergenic 45.0%
!!! CCCTCAGCTGGGTTTTTTTT+AGG - chr4_1:81500196-81500215 Msa0577280:intron 45.0%
CCCTAAAAAAAACCCAGCTG+AGG + chr4_1:81500200-81500219 None:intergenic 45.0%
AAGTTCACCAGCCACTATAG+TGG + chr4_1:81500243-81500262 None:intergenic 45.0%
ATCAGCTTCCCGTGCAAATA+TGG + chr4_1:81500373-81500392 None:intergenic 45.0%
CCTATAGTTGACTGAACCAG+GGG + chr4_1:81500401-81500420 None:intergenic 45.0%
CTCCTATAGTTGACTGAACC+AGG + chr4_1:81500403-81500422 None:intergenic 45.0%
CTGGTTCAGTCAACTATAGG+AGG - chr4_1:81500401-81500420 Msa0577280:intron 45.0%
TACAGGTACATCTGGTGATC+AGG - chr4_1:81500579-81500598 Msa0577280:CDS 45.0%
GGCCCATCACAAAAATGAAG+AGG - chr4_1:81500600-81500619 Msa0577280:CDS 45.0%
!! CCGCCTCTTCATTTTTGTGA+TGG + chr4_1:81500606-81500625 None:intergenic 45.0%
AGCAGACAAATGACTGTCAG+TGG - chr4_1:81500685-81500704 Msa0577280:CDS 45.0%
!! GGAAAGATGGCAATTTTGGC+TGG + chr4_1:81500792-81500811 None:intergenic 45.0%
! TCAGAGTTAGCCTCAACAGA+GGG + chr4_1:81500880-81500899 None:intergenic 45.0%
GACTGATCCTTGTTCCGTAA+TGG - chr4_1:81500945-81500964 Msa0577280:CDS 45.0%
TCACACACCATTACGGAACA+AGG + chr4_1:81500955-81500974 None:intergenic 45.0%
TTTAGACTCTGAGCTTCAGG+AGG + chr4_1:81501144-81501163 None:intergenic 45.0%
TTACCAGACACTGCAACACA+TGG - chr4_1:81501318-81501337 Msa0577280:CDS 45.0%
! CCCCAAACTTCAGAAGCTTT+TGG - chr4_1:81501860-81501879 Msa0577280:intron 45.0%
GATCCTATGGGTGATAGTAC+AGG - chr4_1:81501954-81501973 Msa0577280:CDS 45.0%
! ATAGGACCTAAAGGTGTGAC+TGG + chr4_1:81502140-81502159 None:intergenic 45.0%
TGATTTCTCCCTGTTGGACA+AGG + chr4_1:81502204-81502223 None:intergenic 45.0%
TTCAGGTGATTTCTCCCTGT+TGG + chr4_1:81502210-81502229 None:intergenic 45.0%
GAGAAATCACCTGAAACACC+TGG - chr4_1:81502215-81502234 Msa0577280:CDS 45.0%
CTGAAACACCTGGATGTTGT+CGG - chr4_1:81502225-81502244 Msa0577280:CDS 45.0%
ACAGATCAACTTGATGCCCT+TGG - chr4_1:81502269-81502288 Msa0577280:CDS 45.0%
!! TTTTCGGGAACTAACGCCAA+GGG + chr4_1:81502288-81502307 None:intergenic 45.0%
AAAAACCCAAGAGGATGCAG+AGG - chr4_1:81502320-81502339 Msa0577280:CDS 45.0%
AAAACCCAAGAGGATGCAGA+GGG - chr4_1:81502321-81502340 Msa0577280:CDS 45.0%
! TGTCAACGACACTCCTCTTT+TGG - chr4_1:81502502-81502521 Msa0577280:intron 45.0%
! AACGACACTCCTCTTTTGGA+TGG - chr4_1:81502506-81502525 Msa0577280:intron 45.0%
! TCCTCTTTTGGATGGAAGTC+AGG - chr4_1:81502514-81502533 Msa0577280:intron 45.0%
ACCTGACTTCCATCCAAAAG+AGG + chr4_1:81502518-81502537 None:intergenic 45.0%
TGTTTCAGATGCTTTCCTGC+AGG + chr4_1:81503248-81503267 None:intergenic 45.0%
! TTCTAGGAGTGGAACACATC+AGG - chr4_1:81503527-81503546 Msa0577280:CDS 45.0%
GGCAACTACTTCCAGGTATT+TGG + chr4_1:81503567-81503586 None:intergenic 45.0%
AATACCTGGAAGTAGTTGCC+TGG - chr4_1:81503567-81503586 Msa0577280:CDS 45.0%
!! TCCTACTGCTAGAAAGTACC+AGG + chr4_1:81503588-81503607 None:intergenic 45.0%
! TGGTACTTTCTAGCAGTAGG+AGG - chr4_1:81503587-81503606 Msa0577280:CDS 45.0%
GGAATTTCGGATCACGTCCA+TGG + chr4_1:81498200-81498219 None:intergenic 50.0%
CGTTCCGTTTGCGGGAATTT+CGG + chr4_1:81498213-81498232 None:intergenic 50.0%
! CCCGTTCCTGTTCCTTTTTG+TGG - chr4_1:81498403-81498422 Msa0577280:three_prime_UTR 50.0%
TCTACCTTGTGCTACCACCA+AGG - chr4_1:81498567-81498586 Msa0577280:intron 50.0%
AATAAATTCGCCTCGCACGG+AGG + chr4_1:81498594-81498613 None:intergenic 50.0%
GGGAATAAATTCGCCTCGCA+CGG + chr4_1:81498597-81498616 None:intergenic 50.0%
TACTTCCCGATCAGAATCGC+CGG + chr4_1:81498632-81498651 None:intergenic 50.0%
!!! GTTTTATGCTCCGTCGGTTC+TGG - chr4_1:81498663-81498682 Msa0577280:CDS 50.0%
!!! CTGCCATTTTCCAGAACCGA+CGG + chr4_1:81498676-81498695 None:intergenic 50.0%
GGAAAATGGCAGTGTGAGGA+CGG - chr4_1:81498684-81498703 Msa0577280:intron 50.0%
TGAAAACGGCGATACACCTG+TGG - chr4_1:81498753-81498772 Msa0577280:intron 50.0%
ATACACCTGTGGATGATGCG+AGG - chr4_1:81498764-81498783 Msa0577280:intron 50.0%
TTGATCCTCGCATCATCCAC+AGG + chr4_1:81498772-81498791 None:intergenic 50.0%
TAGAAGCTAGCTGCGATGTG+AGG - chr4_1:81498914-81498933 Msa0577280:intron 50.0%
TAGCTGCGATGTGAGGAATG+TGG - chr4_1:81498921-81498940 Msa0577280:intron 50.0%
CGATGTGAGGAATGTGGAAG+AGG - chr4_1:81498927-81498946 Msa0577280:intron 50.0%
CTGAAAATTCAAGCGAGGGC+CGG - chr4_1:81498995-81499014 Msa0577280:intron 50.0%
ACATGTGCGACGGAGCTAAT+TGG - chr4_1:81499319-81499338 Msa0577280:intron 50.0%
CGACGGAGCTAATTGGCTAT+TGG - chr4_1:81499326-81499345 Msa0577280:intron 50.0%
CAAGAAGGTTATGGGAGGTC+AGG - chr4_1:81499515-81499534 Msa0577280:CDS 50.0%
TGCACCATGGGTGATACCAA+TGG - chr4_1:81499603-81499622 Msa0577280:CDS 50.0%
GCACCATGGGTGATACCAAT+GGG - chr4_1:81499604-81499623 Msa0577280:CDS 50.0%
TGATACCAATGGGCCAGAAC+TGG - chr4_1:81499614-81499633 Msa0577280:CDS 50.0%
! GGATCTGGCTGCAGATTTTC+TGG - chr4_1:81499731-81499750 Msa0577280:CDS 50.0%
GCTAAGGCTGCTGATCACAA+TGG - chr4_1:81499831-81499850 Msa0577280:CDS 50.0%
GTAACCTGGGATGTCTTTGC+AGG - chr4_1:81500339-81500358 Msa0577280:intron 50.0%
TGAACCTGCAAAGACATCCC+AGG + chr4_1:81500346-81500365 None:intergenic 50.0%
CCCCTGGTTCAGTCAACTAT+AGG - chr4_1:81500398-81500417 Msa0577280:intron 50.0%
TCGGAGGAGTCTTGGAAAGA+TGG + chr4_1:81500805-81500824 None:intergenic 50.0%
TTGGCAATTCGGAGGAGTCT+TGG + chr4_1:81500813-81500832 None:intergenic 50.0%
CTTATCTGCACCCTCTGTTG+AGG - chr4_1:81500867-81500886 Msa0577280:CDS 50.0%
! CTCAGAGTTAGCCTCAACAG+AGG + chr4_1:81500881-81500900 None:intergenic 50.0%
GGTTCTCACCGGTGTAGTAT+TGG + chr4_1:81501460-81501479 None:intergenic 50.0%
!! AGGTTCTAAATCGCTGCCCA+TGG - chr4_1:81501624-81501643 Msa0577280:intron 50.0%
!! GGTTCTAAATCGCTGCCCAT+GGG - chr4_1:81501625-81501644 Msa0577280:intron 50.0%
TACACCTGCCTTGTCCAACA+GGG - chr4_1:81502193-81502212 Msa0577280:CDS 50.0%
CGACAACATCCAGGTGTTTC+AGG + chr4_1:81502227-81502246 None:intergenic 50.0%
!! CTTTTCGGGAACTAACGCCA+AGG + chr4_1:81502289-81502308 None:intergenic 50.0%
CTTGGCGTTAGTTCCCGAAA+AGG - chr4_1:81502287-81502306 Msa0577280:CDS 50.0%
AAAGCCCTCTGCATCCTCTT+GGG + chr4_1:81502328-81502347 None:intergenic 50.0%
AAGGTCTTGGGCAACCTCTT+CGG + chr4_1:81502429-81502448 None:intergenic 50.0%
GGTTGCCCAAGACCTTATGA+AGG - chr4_1:81502433-81502452 Msa0577280:intron 50.0%
CACCATAGGGGGACACTATA+GGG + chr4_1:81502977-81502996 None:intergenic 50.0%
TCACCATAGGGGGACACTAT+AGG + chr4_1:81502978-81502997 None:intergenic 50.0%
TTCAGGTGAAAGAAGCCTGC+AGG - chr4_1:81503230-81503249 Msa0577280:CDS 50.0%
AGTACCAGGCAACTACTTCC+AGG + chr4_1:81503574-81503593 None:intergenic 50.0%
! GCCTGGTACTTTCTAGCAGT+AGG - chr4_1:81503584-81503603 Msa0577280:CDS 50.0%
AGATCGGACGTTCCGTTTGC+GGG + chr4_1:81498221-81498240 None:intergenic 55.0%
GAGATCGGACGTTCCGTTTG+CGG + chr4_1:81498222-81498241 None:intergenic 55.0%
AGCTTTGCTGCGGGTGAATG+TGG + chr4_1:81498260-81498279 None:intergenic 55.0%
TGAACCGGACCAGATCTGAC+CGG + chr4_1:81498296-81498315 None:intergenic 55.0%
ACAGGAACGGGAACGGAAGA+GGG + chr4_1:81498394-81498413 None:intergenic 55.0%
AACAGGAACGGGAACGGAAG+AGG + chr4_1:81498395-81498414 None:intergenic 55.0%
! AGGTCCTTGGTGGTAGCACA+AGG + chr4_1:81498574-81498593 None:intergenic 55.0%
!! GCTCCGTCGGTTCTGGAAAA+TGG - chr4_1:81498670-81498689 Msa0577280:CDS 55.0%
TGGCCCATTGGTATCACCCA+TGG + chr4_1:81499610-81499629 None:intergenic 55.0%
! AGATGCCAGTTCTGGCCCAT+TGG + chr4_1:81499622-81499641 None:intergenic 55.0%
GGGAAGCTGATCAAAACCCC+TGG - chr4_1:81500382-81500401 Msa0577280:intron 55.0%
! GTTGCAGTGTCTGGTAACGC+AGG + chr4_1:81501315-81501334 None:intergenic 55.0%
CCACCATGTGTTGCAGTGTC+TGG + chr4_1:81501324-81501343 None:intergenic 55.0%
CCAGACACTGCAACACATGG+TGG - chr4_1:81501321-81501340 Msa0577280:CDS 55.0%
GACACTGCAACACATGGTGG+TGG - chr4_1:81501324-81501343 Msa0577280:CDS 55.0%
GTACACCTGCCTTGTCCAAC+AGG - chr4_1:81502192-81502211 Msa0577280:CDS 55.0%
TTCTCCCTGTTGGACAAGGC+AGG + chr4_1:81502200-81502219 None:intergenic 55.0%
GACAAGCTCCGACAACATCC+AGG + chr4_1:81502236-81502255 None:intergenic 55.0%
CAAAGCCCTCTGCATCCTCT+TGG + chr4_1:81502329-81502348 None:intergenic 55.0%
TCAGCTGCTAGATGCCGAAG+AGG - chr4_1:81502412-81502431 Msa0577280:CDS 55.0%
AATGTGGATGAGGCGGGTGG+CGG + chr4_1:81498244-81498263 None:intergenic 60.0%
GTGAATGTGGATGAGGCGGG+TGG + chr4_1:81498247-81498266 None:intergenic 60.0%
CGGGTGAATGTGGATGAGGC+GGG + chr4_1:81498250-81498269 None:intergenic 60.0%
GCGGGTGAATGTGGATGAGG+CGG + chr4_1:81498251-81498270 None:intergenic 60.0%
GCTGCGGGTGAATGTGGATG+AGG + chr4_1:81498254-81498273 None:intergenic 60.0%
GGTACGGTGAGCTTTGCTGC+GGG + chr4_1:81498269-81498288 None:intergenic 60.0%
GGGTACGGTGAGCTTTGCTG+CGG + chr4_1:81498270-81498289 None:intergenic 60.0%
AAAGCTCACCGTACCCGCTC+CGG - chr4_1:81498274-81498293 Msa0577280:three_prime_UTR 60.0%
AGATCTGACCGGAGCGGGTA+CGG + chr4_1:81498285-81498304 None:intergenic 60.0%
GGCTGTGCCTTAGAGCAGCT+TGG - chr4_1:81499852-81499871 Msa0577280:CDS 60.0%
GGCCCTATAGTGTCCCCCTA+TGG - chr4_1:81502972-81502991 Msa0577280:CDS 60.0%
!! ATGAGGCGGGTGGCGGAGAT+CGG + chr4_1:81498237-81498256 None:intergenic 65.0%
GTACCCGCTCCGGTCAGATC+TGG - chr4_1:81498284-81498303 Msa0577280:three_prime_UTR 65.0%
GGACCAGATCTGACCGGAGC+GGG + chr4_1:81498290-81498309 None:intergenic 65.0%
CGGACCAGATCTGACCGGAG+CGG + chr4_1:81498291-81498310 None:intergenic 65.0%
CGCTCCGGTCAGATCTGGTC+CGG - chr4_1:81498289-81498308 Msa0577280:three_prime_UTR 65.0%
TATTCCCCACTTGCCGGCGC+CGG - chr4_1:81498610-81498629 Msa0577280:CDS 65.0%
CCTCGCACGGAGGTCCTTGG+TGG + chr4_1:81498584-81498603 None:intergenic 70.0%
CCACCAAGGACCTCCGTGCG+AGG - chr4_1:81498581-81498600 Msa0577280:intron 70.0%
TCGCCTCGCACGGAGGTCCT+TGG + chr4_1:81498587-81498606 None:intergenic 70.0%
!! AATCGCCGGCGCCGGCAAGT+GGG + chr4_1:81498618-81498637 None:intergenic 70.0%
CCGATCAGAATCGCCGGCGC+CGG + chr4_1:81498626-81498645 None:intergenic 70.0%
!! CCGGCGCCGGCGATTCTGAT+CGG - chr4_1:81498623-81498642 Msa0577280:CDS 70.0%
!! CGGCGCCGGCGATTCTGATC+GGG - chr4_1:81498624-81498643 Msa0577280:CDS 70.0%
!! ATCGCCGGCGCCGGCAAGTG+GGG + chr4_1:81498617-81498636 None:intergenic 75.0%
!! GAATCGCCGGCGCCGGCAAG+TGG + chr4_1:81498619-81498638 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81498181 81503752 81498181 ID=Msa0577280;Name=Msa0577280
chr4_1 mRNA 81498181 81503752 81498181 ID=Msa0577280-mRNA-1;Parent=Msa0577280;Name=Msa0577280-mRNA-1;_AED=0.05;_eAED=0.05;_QI=18|1|1|1|1|1|9|264|1165
chr4_1 exon 81498181 81498525 81498181 ID=Msa0577280-mRNA-1:exon:29374;Parent=Msa0577280-mRNA-1
chr4_1 exon 81498600 81498698 81498600 ID=Msa0577280-mRNA-1:exon:29373;Parent=Msa0577280-mRNA-1
chr4_1 exon 81499398 81500095 81499398 ID=Msa0577280-mRNA-1:exon:29372;Parent=Msa0577280-mRNA-1
chr4_1 exon 81500205 81500307 81500205 ID=Msa0577280-mRNA-1:exon:29371;Parent=Msa0577280-mRNA-1
chr4_1 exon 81500463 81501349 81500463 ID=Msa0577280-mRNA-1:exon:29370;Parent=Msa0577280-mRNA-1
chr4_1 exon 81501470 81501572 81501470 ID=Msa0577280-mRNA-1:exon:29369;Parent=Msa0577280-mRNA-1
chr4_1 exon 81501863 81502449 81501863 ID=Msa0577280-mRNA-1:exon:29368;Parent=Msa0577280-mRNA-1
chr4_1 exon 81502684 81502774 81502684 ID=Msa0577280-mRNA-1:exon:29367;Parent=Msa0577280-mRNA-1
chr4_1 exon 81502886 81503752 81502886 ID=Msa0577280-mRNA-1:exon:29366;Parent=Msa0577280-mRNA-1
chr4_1 five_prime_UTR 81503735 81503752 81503735 ID=Msa0577280-mRNA-1:five_prime_utr;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81502886 81503734 81502886 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81502684 81502774 81502684 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81501863 81502449 81501863 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81501470 81501572 81501470 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81500463 81501349 81500463 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81500205 81500307 81500205 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81499398 81500095 81499398 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81498600 81498698 81498600 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 CDS 81498445 81498525 81498445 ID=Msa0577280-mRNA-1:cds;Parent=Msa0577280-mRNA-1
chr4_1 three_prime_UTR 81498181 81498444 81498181 ID=Msa0577280-mRNA-1:three_prime_utr;Parent=Msa0577280-mRNA-1
Gene Sequence

>Msa0577280

ATGGACGTGATCCGAAATTCCCGCAAACGGAACGTCCGATCTCCGCCACCCGCCTCATCCACATTCACCCGCAGCAAAGCTCACCGTACCCGCTCCGGTCAGATCTGGTCCGGTTCAAGTTCCGTTTCAAGTTCCCGCAGCAAAAAGAATCAACCATACTCAGTTCCGGTTCAAGTTCCGTTTCAAGAGGAACCCTCTTCCGTTCCCGTTCCTGTTCCTTTTTGTGGAAACGATGTAACCAAAGGACTTTCAGATTCGGAATTCAAGAATCTAATTCATGATATTTGCAATGAATATTGTGAATCAGATTCCGAAGATGACGATCTTGTTAAGGATCCTTTCAAGAAAGCAAAACGTGATGATGCTAATCTACCTTGTGCTACCACCAAGGACCTCCGTGCGAGGCGAATTTATTCCCCACTTGCCGGCGCCGGCGATTCTGATCGGGAAGTAGCTGAATTAGGGTTTTATGCTCCGTCGGTTCTGGAAAATGGCAGTGTGAGGACGGAATCAGCTGAAATTTCTGAATTAGGGTTTGGATCGCCATCAATTGCTGAAAACGGCGATACACCTGTGGATGATGCGAGGATCAAATCTAATAAGATTCTCGACGAGCCTCTGAATTTTGATAGGGGAATCACCAAGCTGTCAGATGAAGATTTGGGCGAAGAATCATTACAAACGACACCACCTAATGATGTCATCTATGTAGGTTTAGAAGCTAGCTGCGATGTGAGGAATGTGGAAGAGGCAGACACACTTGTTGTCAAAGATATGTCTCCTGCTGCTAATGTAGCTGAAAATTCAAGCGAGGGCCGGAAAAATAGCTCTGTTCAAAGGAGTAAATCTGTACTCAAACCGCGTTTACCTTTGAAATTGTTCAAAGCCCCAGGTTCATTCAATTACAAAAGGCTAATGGATGCTATGGGAGATAATTTCGGAATACCGAAATTGGGTCATTGTCATGAAGACAAGAAGGTTATGGGAGGTCAGGGATTGAAACTTCCTTTGACATCTGATAATCATGAAGGTTCTAAGCCTGAAACTAAGATTGATAGTTGCACCATGGGTGATACCAATGGGCCAGAACTGGCATCTTCACCAATGCAAGTAGATACTGAGAAAGTGAATGGCGAGTGTTTATCTGTGCCATCTGTGGATGATAAATGCTTGAGTGAATCAAAGGTGGATCTGGCTGCAGATTTTCTGGGTGCTAGAGATGTTGGTAATGCTTTCGACCACGGTGATGTTAAGCAAGATTCTTTCAACAGGAATAATCTTGACTGTGCTAAGGCTGCTGATCACAATGGCTGTGCCTTAGAGCAGCTTGGTGTTTTGAATGAAGATTGTATACCAATGACCCGTGATACTGAAATATGTGATGATATTGTGCTCAATGGAGACCAAATGCAGAGCATACCTCAGAATGTCAAACCTATGGATCTGACCAGAAACACTCAAGAAAGCACTGTTGAAGCGTTATACCCCAAAGCCCATAAGAGAAATAATCCACTCAAGAGCAAACATGTTCATAAACCATATTTGCACGGGAAGCTGATCAAAACCCCTGGTTCAGTCAACTATAGGAGGTTGCTTCCATATTTTAAGAATCTTCCCAAAGATGACTATGGTACATCTGGTGATCAGGCCCATCACAAAAATGAAGAGGCGGATTTATATGCAAAGGAGTTCCAACTTCCTTTGCCATCTCAAAGTGAAGAAGCTTCTATTAGCAGACAAATGACTGTCAGTGGCTCTATCCATGACAAAGTTGACTTGAATACTTTGGAAAACAACATCTCAGTCAATCCTGCTAATATGTCATCTCATGGCTACCAGCCAAAATTGCCATCTTTCCAAGACTCCTCCGAATTGCCAATACAATTTGACGCAAAAGAAATGGCACATGAGTGCTTATCTGCACCCTCTGTTGAGGCTAACTCTGAGAAAACTGTCATCAGTTCTAAAGATGAATGCTTGAGTGAATCAGCGACTGATCCTTGTTCCGTAATGGTGTGTGATAAGATTGTCACAAATGGTGGGTCACCACCCAAAGAACAAAATTTGCTTAATGTCAATAATGATATCTCCAACCTTTCATTTGAGCATCATTCAAGCAATGAGAAAGGATTCACTGAAAGAAAGCAGTTTGTGAGTCTGGAGAAGCATGAATCTGTTAGCAGATGTCCTCCTGAAGCTCAGAGTCTAAATCAATTGGATCCTAATATGTTAGATGCTGAAGAGAATGAAACAAGCAGCCATGCAATTTGCAAAAATGACGATGTTGTGGTTTTATGTCACGTCACCATCTCAAATGAAGAATCTAGTTCACAATCAGAGAAGATTATTTCTGAAAAACCTGCGTTACCAGACACTGCAACACATGGTGGTGGCAAAGAAAAAAGTCTTCTGAATGGCCTAGTTAATGGTTCAAAGATGCCTTCAAAAGGAAGCAATAACAAAAAGGCTAGTTTTGCTGGTGAGATGAAAAATGGTTTGGAATCCAATACTACACCGGTTCTAAATCGCTGCCCATGGGTTAAGTTTTTAAAACATGCTGGGTCTTTGAACTACAAAAGATTGCTTCCGATTTTGTTGGAAAATGAGAAGTCTAATTCCTGTGCTTCGAATAATGATCATCGCCCCAAACTTCAGAAGCTTTTGGATCACACACCATTTCTGCCAATTTCAGATTCAAACTTGCAAGTAACTCCTGGAAGTGATTCAAATGATTGTGATCCTATGGGTGATAGTACAGGAAATTCTGGTGCTCAGCAAGAAACAGAGTTGCAAGCTTGTGACTTAAATAGTGATAGTTTAAGCCCAAGATCTCAACTACAAGATAAACAGATTGTGTCAAATAGACCCTACTATCTCGAGAACTCCTTTGTTTCACCAATTTCTGTTCATAGAGATTTACCAGTCACACCTTTAGGTCCTATAGCTGATCAAGTTGTCACTAGAGAAGAAGTGGGTACACCTGCCTTGTCCAACAGGGAGAAATCACCTGAAACACCTGGATGTTGTCGGAGCTTGTCCGAAATGAAAGTCACAGATCAACTTGATGCCCTTGGCGTTAGTTCCCGAAAAGGAATTTTGAGAAAAAACCCAAGAGGATGCAGAGGGCTTTGCACATGTTTGAACTGTGTTTCTTTTCGCCTTCATGCAGAAAGAGCATTTGAATTTTCTAGAAATCAGCTGCTAGATGCCGAAGAGGTTGCCCAAGACCTTATGAAGGAAGTATCCCATCTCAGAAACATGTTGTTAGAAAGATGTACTGATAATGTCAACGACACTCCTCTTTTGGATGGAAGTCAGGTGAAAGAAGCCTGCAGGAAAGCATCTGAAACAGAACAACTTGCAAAGGATCGACTTAGCCAGATGAATGATGATCTTAACATACATTGCAGAACACCTAGTCTGCAGCGACCAAGTGTTAAGTTTTCAGATCGTGTTGAGGAAAAGGTCATTCAACCAAACAGATTAAAAAATGGTTAA
Protein sequence

>Msa0577280

MDVIRNSRKRNVRSPPPASSTFTRSKAHRTRSGQIWSGSSSVSSSRSKKNQPYSVPVQVPFQEEPSSVPVPVPFCGNDVTKGLSDSEFKNLIHDICNEYCESDSEDDDLVKDPFKKAKRDDANLPCATTKDLRARRIYSPLAGAGDSDREVAELGFYAPSVLENGSVRTESAEISELGFGSPSIAENGDTPVDDARIKSNKILDEPLNFDRGITKLSDEDLGEESLQTTPPNDVIYVGLEASCDVRNVEEADTLVVKDMSPAANVAENSSEGRKNSSVQRSKSVLKPRLPLKLFKAPGSFNYKRLMDAMGDNFGIPKLGHCHEDKKVMGGQGLKLPLTSDNHEGSKPETKIDSCTMGDTNGPELASSPMQVDTEKVNGECLSVPSVDDKCLSESKVDLAADFLGARDVGNAFDHGDVKQDSFNRNNLDCAKAADHNGCALEQLGVLNEDCIPMTRDTEICDDIVLNGDQMQSIPQNVKPMDLTRNTQESTVEALYPKAHKRNNPLKSKHVHKPYLHGKLIKTPGSVNYRRLLPYFKNLPKDDYGTSGDQAHHKNEEADLYAKEFQLPLPSQSEEASISRQMTVSGSIHDKVDLNTLENNISVNPANMSSHGYQPKLPSFQDSSELPIQFDAKEMAHECLSAPSVEANSEKTVISSKDECLSESATDPCSVMVCDKIVTNGGSPPKEQNLLNVNNDISNLSFEHHSSNEKGFTERKQFVSLEKHESVSRCPPEAQSLNQLDPNMLDAEENETSSHAICKNDDVVVLCHVTISNEESSSQSEKIISEKPALPDTATHGGGKEKSLLNGLVNGSKMPSKGSNNKKASFAGEMKNGLESNTTPVLNRCPWVKFLKHAGSLNYKRLLPILLENEKSNSCASNNDHRPKLQKLLDHTPFLPISDSNLQVTPGSDSNDCDPMGDSTGNSGAQQETELQACDLNSDSLSPRSQLQDKQIVSNRPYYLENSFVSPISVHRDLPVTPLGPIADQVVTREEVGTPALSNREKSPETPGCCRSLSEMKVTDQLDALGVSSRKGILRKNPRGCRGLCTCLNCVSFRLHAERAFEFSRNQLLDAEEVAQDLMKEVSHLRNMLLERCTDNVNDTPLLDGSQVKEACRKASETEQLAKDRLSQMNDDLNIHCRTPSLQRPSVKFSDRVEEKVIQPNRLKNG*