AlfalfaGEDB | Msa0659150

M. sativa cultivar zhongmu-4 / Msa0659150

KEGG: K11253
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464
PFAM: PF00125.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659150	KYP58465.1	100.000	136	0	0	1	136	5	140	1.76e-92	273

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659150	sp\|P68428\|H32_WHEAT	100.000	136	0	0	1	136	1	136	5.15e-95	272

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016040	Msa0659150	0.812769	3.588763e-51	-8.615850e-47
Msa0018920	Msa0659150	0.813959	1.965437e-51	-8.615850e-47
Msa0019930	Msa0659150	0.822367	2.461008e-53	-8.615850e-47
Msa0027280	Msa0659150	0.827885	1.221671e-54	-8.615850e-47
Msa0030070	Msa0659150	0.820881	5.426130e-53	-8.615850e-47
Msa0030690	Msa0659150	0.810836	9.454740e-51	-8.615850e-47
Msa0032980	Msa0659150	0.823198	1.576265e-53	-8.615850e-47
Msa0034830	Msa0659150	0.807615	4.635718e-50	-8.615850e-47
Msa0035140	Msa0659150	0.837058	6.504021e-57	-8.615850e-47
Msa0037740	Msa0659150	0.805759	1.142961e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659150	MtrunA17_Chr5g0410931	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr5g0410951	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr8g0383851	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr8g0383781	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr8g0390361	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr2g0295191	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr7g0237661	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr4g0033041	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr7g0218971	100.000	136	1	136	1	136	3.79e-96	272
Msa0659150	MtrunA17_Chr4g0046301	100.000	136	1	136	1	136	3.79e-96	272

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0659150	AT1G09200.1	99.265	136	1	1	136	1	136	1.62e-95	271
Msa0659150	AT5G65360.1	99.265	136	1	1	136	1	136	1.62e-95	271
Msa0659150	AT5G10390.1	99.265	136	1	1	136	1	136	1.62e-95	271
Msa0659150	AT5G10400.1	99.265	136	1	1	136	1	136	1.62e-95	271
Msa0659150	AT3G27360.1	99.265	136	1	1	136	1	136	1.62e-95	271
Msa0659150	AT4G40030.3	97.059	136	4	1	136	1	136	3.59e-93	265
Msa0659150	AT4G40030.1	97.059	136	4	1	136	1	136	3.59e-93	265
Msa0659150	AT4G40030.4	97.059	136	4	1	136	1	136	3.59e-93	265
Msa0659150	AT5G10980.1	97.059	136	4	1	136	1	136	3.59e-93	265
Msa0659150	AT4G40040.2	97.059	136	4	1	136	1	136	3.59e-93	265

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Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAGCTCCGGCGACCGG+AGG	0.599225	4_3:+69465084	Msa0659150:CDS
AACTGGCAACCAAAGCCGCT+CGG	0.560134	4_3:+69465061	Msa0659150:CDS
AAGAAGAATCAGAGGAGAGA+GGG	0.573886	4_3:+69465386	Msa0659150:CDS
AAGCCACACAGATTCAGACC+TGG	0.672238	4_3:+69465114	Msa0659150:CDS
ACTGCTCGCAAGTCCACCGG+CGG	0.647164	4_3:+69465021	Msa0659150:CDS
AGAAGCGGCTGAAGCTTACT+TGG	0.416086	4_3:+69465284	Msa0659150:CDS
AGCCGCTCGGAAATCAGCTC+CGG	0.498921	4_3:+69465074	Msa0659150:CDS
AGCTGATTTCCGAGCGGCTT+TGG	0.366844	4_3:-69465070	None:intergenic
AGGCATGATTGTAACTCTCT+TGG	0.461766	4_3:-69465346	None:intergenic
ATTCCAGAGCAGTGCTGTTT+CGG	0.340103	4_3:+69465254	Msa0659150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACGTTGGAATGGAAGTTTA+CGG	-	chr4_3:69465194-69465213	None:intergenic	40.0%
AGGCATGATTGTAACTCTCT+TGG	-	chr4_3:69465349-69465368	None:intergenic	40.0%
GAGAGTTACAATCATGCCTA+AGG	+	chr4_3:69465350-69465369	Msa0659150:CDS	40.0%
AAGAAGAATCAGAGGAGAGA+GGG	+	chr4_3:69465386-69465405	Msa0659150:CDS	40.0%
TCTCTCAAAGCAACTGTTCC+AGG	-	chr4_3:69465135-69465154	None:intergenic	45.0%
TCACGAACAAGACGTTGGAA+TGG	-	chr4_3:69465204-69465223	None:intergenic	45.0%
CGATTTCACGAACAAGACGT+TGG	-	chr4_3:69465209-69465228	None:intergenic	45.0%
TGTTTCGGCTCTTCAAGAAG+CGG	+	chr4_3:69465269-69465288	Msa0659150:CDS	45.0%
CTTGCAAGCTGAATGTCCTT+AGG	-	chr4_3:69465369-69465388	None:intergenic	45.0%
CAGCTTGCAAGAAGAATCAG+AGG	+	chr4_3:69465378-69465397	Msa0659150:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	69465003	69465413	69465003	ID=Msa0659150;Name=Msa0659150
chr4_3	mRNA	69465003	69465413	69465003	ID=Msa0659150-mRNA-1;Parent=Msa0659150;Name=Msa0659150-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
chr4_3	exon	69465003	69465413	69465003	ID=Msa0659150-mRNA-1:exon:20362;Parent=Msa0659150-mRNA-1
chr4_3	CDS	69465003	69465413	69465003	ID=Msa0659150-mRNA-1:cds;Parent=Msa0659150-mRNA-1

Gene Sequence

>Msa0659150

ATGGCACGTACCAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAACTGGCAACCAAAGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTGAGATTCCAGAGCAGTGCTGTTTCGGCTCTTCAAGAAGCGGCTGAAGCTTACTTGGTCGGTCTCTTTGAAGATACTAATCTTTGTGCAATTCATGCCAAGAGAGTTACAATCATGCCTAAGGACATTCAGCTTGCAAGAAGAATCAGAGGAGAGAGGGCTTAG

Protein sequence

>Msa0659150

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*