AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0659150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659150 MtrunA17_Chr5g0410931 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr5g0410951 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr8g0383851 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr8g0383781 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr8g0390361 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr2g0295191 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr7g0237661 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr4g0033041 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr7g0218971 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr4g0046301 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr8g0364801 100.000 136 0 0 1 136 1 136 3.79e-96 272
Msa0659150 MtrunA17_Chr4g0054161 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659150 MtrunA17_Chr8g0363741 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659150 MtrunA17_Chr1g0156021 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa0659150 MtrunA17_Chr4g0054151 97.059 136 4 0 1 136 4 139 2.61e-93 265
Msa0659150 MtrunA17_Chr8g0388121 96.324 136 5 0 1 136 1 136 1.09e-92 264
Msa0659150 MtrunA17_Chr2g0319141 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0659150 MtrunA17_Chr2g0319151 89.706 136 14 0 1 136 1 136 1.10e-86 248
Msa0659150 MtrunA17_Chr2g0319131 85.616 146 11 1 1 136 1 146 1.38e-82 238
Msa0659150 MtrunA17_Chr2g0319121 84.559 136 19 2 1 135 1 135 5.82e-75 219
Msa0659150 MtrunA17_Chr7g0218961 86.607 112 15 0 25 136 2 113 8.01e-66 195
Msa0659150 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 5.47e-56 171
Msa0659150 MtrunA17_Chr5g0407311 67.241 116 35 2 1 116 1 113 1.29e-45 144
Msa0659150 MtrunA17_Chr7g0223001 58.647 133 41 3 3 134 27 146 5.46e-43 139
Msa0659150 MtrunA17_Chr7g0240791 100.000 66 0 0 1 66 1 66 9.61e-41 133
Msa0659150 MtrunA17_Chr8g0347231 55.102 98 43 1 37 133 28 125 3.80e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659150 AT1G09200.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659150 AT5G65360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659150 AT5G10390.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659150 AT5G10400.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659150 AT3G27360.1 99.265 136 1 0 1 136 1 136 1.62e-95 271
Msa0659150 AT4G40030.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40030.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40030.4 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT5G10980.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40040.2 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40040.3 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40040.1 97.059 136 4 0 1 136 1 136 3.59e-93 265
Msa0659150 AT4G40030.2 97.059 136 4 0 1 136 29 164 7.05e-93 266
Msa0659150 AT5G65350.1 94.853 136 7 0 1 136 1 136 5.30e-91 260
Msa0659150 AT1G75600.1 93.382 136 9 0 1 136 1 136 1.25e-89 256
Msa0659150 AT1G13370.1 93.382 136 9 0 1 136 1 136 5.55e-89 254
Msa0659150 AT1G19890.1 89.781 137 13 1 1 136 1 137 1.07e-83 241
Msa0659150 AT5G12910.1 71.111 135 34 2 1 135 1 130 9.90e-67 198
Msa0659150 AT1G01370.2 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659150 AT1G01370.1 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659150 AT1G01370.4 53.982 113 49 2 24 134 63 174 2.97e-31 110
Msa0659150 AT1G01370.3 53.982 113 49 2 24 134 63 174 2.97e-31 110

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGTTGCCAGTTGCTTC+CGG 0.239809 4_3:-69465053 None:intergenic
TTTGGTTGCCAGTTGCTTCC+GGG 0.293951 4_3:-69465052 None:intergenic
TCTCTCAAAGCAACTGTTCC+AGG 0.333816 4_3:-69465132 None:intergenic
ATTCCAGAGCAGTGCTGTTT+CGG 0.340103 4_3:+69465254 Msa0659150:CDS
AGCTGATTTCCGAGCGGCTT+TGG 0.366844 4_3:-69465070 None:intergenic
CAAGGCTCCCCGGAAGCAAC+TGG 0.376550 4_3:+69465044 Msa0659150:CDS
AGAAGCGGCTGAAGCTTACT+TGG 0.416086 4_3:+69465284 Msa0659150:CDS
GAGCCGAAACAGCACTGCTC+TGG 0.422418 4_3:-69465257 None:intergenic
TTCTTCACTCCTCCGGTCGC+CGG 0.442119 4_3:-69465093 None:intergenic
GACGTTGGAATGGAAGTTTA+CGG 0.458530 4_3:-69465191 None:intergenic
AGGCATGATTGTAACTCTCT+TGG 0.461766 4_3:-69465346 None:intergenic
CGGAAATCAGCTCCGGCGAC+CGG 0.461897 4_3:+69465081 Msa0659150:CDS
CTTGCAAGCTGAATGTCCTT+AGG 0.462646 4_3:-69465366 None:intergenic
CCACCGGCGGCAAGGCTCCC+CGG 0.480154 4_3:+69465034 Msa0659150:CDS
TCGCAAGTCCACCGGCGGCA+AGG 0.488583 4_3:+69465026 Msa0659150:CDS
AGCCGCTCGGAAATCAGCTC+CGG 0.498921 4_3:+69465074 Msa0659150:CDS
GTGTGGCTTCTTCACTCCTC+CGG 0.499925 4_3:-69465100 None:intergenic
TTGGTTGCCAGTTGCTTCCG+GGG 0.522282 4_3:-69465051 None:intergenic
CTTCCGGGGAGCCTTGCCGC+CGG 0.543290 4_3:-69465037 None:intergenic
CCGGGGAGCCTTGCCGCCGG+TGG 0.548377 4_3:-69465034 None:intergenic
GGACTTGCGAGCAGTCTGCT+TGG 0.551577 4_3:-69465013 None:intergenic
AACTGGCAACCAAAGCCGCT+CGG 0.560134 4_3:+69465061 Msa0659150:CDS
CAGACTGCTCGCAAGTCCAC+CGG 0.562052 4_3:+69465018 Msa0659150:CDS
GCGGCTGAAGCTTACTTGGT+CGG 0.565776 4_3:+69465288 Msa0659150:CDS
GAGAGTTACAATCATGCCTA+AGG 0.573275 4_3:+69465350 Msa0659150:CDS
AAGAAGAATCAGAGGAGAGA+GGG 0.573886 4_3:+69465386 Msa0659150:CDS
TCACGAACAAGACGTTGGAA+TGG 0.598115 4_3:-69465201 None:intergenic
AAATCAGCTCCGGCGACCGG+AGG 0.599225 4_3:+69465084 Msa0659150:CDS
CAAGAAGAATCAGAGGAGAG+AGG 0.603703 4_3:+69465385 Msa0659150:CDS
CGATTTCACGAACAAGACGT+TGG 0.613448 4_3:-69465206 None:intergenic
CAGCTTGCAAGAAGAATCAG+AGG 0.621034 4_3:+69465378 Msa0659150:CDS
ACTGCTCGCAAGTCCACCGG+CGG 0.647164 4_3:+69465021 Msa0659150:CDS
AAGCCACACAGATTCAGACC+TGG 0.672238 4_3:+69465114 Msa0659150:CDS
TGTTTCGGCTCTTCAAGAAG+CGG 0.672573 4_3:+69465269 Msa0659150:CDS
CGCCGGAGCTGATTTCCGAG+CGG 0.685230 4_3:-69465076 None:intergenic
GTTCCAGGTCTGAATCTGTG+TGG 0.688220 4_3:-69465117 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GACGTTGGAATGGAAGTTTA+CGG - chr4_3:69465194-69465213 None:intergenic 40.0%
AGGCATGATTGTAACTCTCT+TGG - chr4_3:69465349-69465368 None:intergenic 40.0%
GAGAGTTACAATCATGCCTA+AGG + chr4_3:69465350-69465369 Msa0659150:CDS 40.0%
AAGAAGAATCAGAGGAGAGA+GGG + chr4_3:69465386-69465405 Msa0659150:CDS 40.0%
TCTCTCAAAGCAACTGTTCC+AGG - chr4_3:69465135-69465154 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG - chr4_3:69465204-69465223 None:intergenic 45.0%
CGATTTCACGAACAAGACGT+TGG - chr4_3:69465209-69465228 None:intergenic 45.0%
!! ATTCCAGAGCAGTGCTGTTT+CGG + chr4_3:69465254-69465273 Msa0659150:CDS 45.0%
TGTTTCGGCTCTTCAAGAAG+CGG + chr4_3:69465269-69465288 Msa0659150:CDS 45.0%
CTTGCAAGCTGAATGTCCTT+AGG - chr4_3:69465369-69465388 None:intergenic 45.0%
CAGCTTGCAAGAAGAATCAG+AGG + chr4_3:69465378-69465397 Msa0659150:CDS 45.0%
CAAGAAGAATCAGAGGAGAG+AGG + chr4_3:69465385-69465404 Msa0659150:CDS 45.0%
TTTGGTTGCCAGTTGCTTCC+GGG - chr4_3:69465055-69465074 None:intergenic 50.0%
CTTTGGTTGCCAGTTGCTTC+CGG - chr4_3:69465056-69465075 None:intergenic 50.0%
AAGCCACACAGATTCAGACC+TGG + chr4_3:69465114-69465133 Msa0659150:CDS 50.0%
GTTCCAGGTCTGAATCTGTG+TGG - chr4_3:69465120-69465139 None:intergenic 50.0%
AGAAGCGGCTGAAGCTTACT+TGG + chr4_3:69465284-69465303 Msa0659150:CDS 50.0%
TTGGTTGCCAGTTGCTTCCG+GGG - chr4_3:69465054-69465073 None:intergenic 55.0%
AACTGGCAACCAAAGCCGCT+CGG + chr4_3:69465061-69465080 Msa0659150:CDS 55.0%
! AGCTGATTTCCGAGCGGCTT+TGG - chr4_3:69465073-69465092 None:intergenic 55.0%
GTGTGGCTTCTTCACTCCTC+CGG - chr4_3:69465103-69465122 None:intergenic 55.0%
GCGGCTGAAGCTTACTTGGT+CGG + chr4_3:69465288-69465307 Msa0659150:CDS 55.0%
GGACTTGCGAGCAGTCTGCT+TGG - chr4_3:69465016-69465035 None:intergenic 60.0%
CAGACTGCTCGCAAGTCCAC+CGG + chr4_3:69465018-69465037 Msa0659150:CDS 60.0%
AGCCGCTCGGAAATCAGCTC+CGG + chr4_3:69465074-69465093 Msa0659150:CDS 60.0%
TTCTTCACTCCTCCGGTCGC+CGG - chr4_3:69465096-69465115 None:intergenic 60.0%
GAGCCGAAACAGCACTGCTC+TGG - chr4_3:69465260-69465279 None:intergenic 60.0%
ACTGCTCGCAAGTCCACCGG+CGG + chr4_3:69465021-69465040 Msa0659150:CDS 65.0%
CAAGGCTCCCCGGAAGCAAC+TGG + chr4_3:69465044-69465063 Msa0659150:CDS 65.0%
! CGCCGGAGCTGATTTCCGAG+CGG - chr4_3:69465079-69465098 None:intergenic 65.0%
CGGAAATCAGCTCCGGCGAC+CGG + chr4_3:69465081-69465100 Msa0659150:CDS 65.0%
AAATCAGCTCCGGCGACCGG+AGG + chr4_3:69465084-69465103 Msa0659150:CDS 65.0%
TCGCAAGTCCACCGGCGGCA+AGG + chr4_3:69465026-69465045 Msa0659150:CDS 70.0%
CTTCCGGGGAGCCTTGCCGC+CGG - chr4_3:69465040-69465059 None:intergenic 75.0%
! CCACCGGCGGCAAGGCTCCC+CGG + chr4_3:69465034-69465053 Msa0659150:CDS 80.0%
! CCGGGGAGCCTTGCCGCCGG+TGG - chr4_3:69465037-69465056 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69465003 69465413 69465003 ID=Msa0659150;Name=Msa0659150
chr4_3 mRNA 69465003 69465413 69465003 ID=Msa0659150-mRNA-1;Parent=Msa0659150;Name=Msa0659150-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|136
chr4_3 exon 69465003 69465413 69465003 ID=Msa0659150-mRNA-1:exon:20362;Parent=Msa0659150-mRNA-1
chr4_3 CDS 69465003 69465413 69465003 ID=Msa0659150-mRNA-1:cds;Parent=Msa0659150-mRNA-1
Gene Sequence

>Msa0659150

ATGGCACGTACCAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCCCCGGAAGCAACTGGCAACCAAAGCCGCTCGGAAATCAGCTCCGGCGACCGGAGGAGTGAAGAAGCCACACAGATTCAGACCTGGAACAGTTGCTTTGAGAGAGATTCGTAAGTATCAGAAGAGCACTGAGCTTCTCATCCGTAAACTTCCATTCCAACGTCTTGTTCGTGAAATCGCTCAAGATTTCAAGACAGATCTGAGATTCCAGAGCAGTGCTGTTTCGGCTCTTCAAGAAGCGGCTGAAGCTTACTTGGTCGGTCTCTTTGAAGATACTAATCTTTGTGCAATTCATGCCAAGAGAGTTACAATCATGCCTAAGGACATTCAGCTTGCAAGAAGAATCAGAGGAGAGAGGGCTTAG

Protein sequence

>Msa0659150

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVSALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*