AlfalfaGEDB | Msa0999430

M. sativa cultivar zhongmu-4 / Msa0999430

KEGG: K09419
GO: GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097305 GO:0140110 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999430	XP_013453094.1	88.352	352	33	4	1	351	1	345	0.0	577

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999430	sp\|Q9LV52\|HSFC1_ARATH	59.906	212	59	3	8	215	14	203	9.66e-80	249

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999430	A0A072UBW9	88.352	352	33	4	1	351	1	345	0.0	577

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TFs/TRs:

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Gene ID	Type	Classification
Msa0999430	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011960	Msa0999430	0.800038	1.738662e-48	-8.615850e-47
Msa0023000	Msa0999430	0.817053	4.025008e-52	-8.615850e-47
Msa0044000	Msa0999430	0.804322	2.284172e-49	-8.615850e-47
Msa0052400	Msa0999430	0.812076	5.085643e-51	-8.615850e-47
Msa0058300	Msa0999430	0.812965	3.251037e-51	-8.615850e-47
Msa0065950	Msa0999430	0.817897	2.597654e-52	-8.615850e-47
Msa0090570	Msa0999430	0.816384	5.686709e-52	-8.615850e-47
Msa0115270	Msa0999430	0.836796	7.585648e-57	-8.615850e-47
Msa0115390	Msa0999430	0.812021	5.227395e-51	-8.615850e-47
Msa0127800	Msa0999430	0.812264	4.626069e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999430	MtrunA17_Chr6g0485571	88.352	352	33	4	1	351	1	345	0.0	577
Msa0999430	MtrunA17_Chr5g0410881	43.802	242	120	5	3	233	24	260	3.92e-58	196
Msa0999430	MtrunA17_Chr3g0135831	44.608	204	112	1	11	213	46	249	3.76e-54	182
Msa0999430	MtrunA17_Chr4g0039891	44.340	212	109	4	6	208	7	218	2.97e-53	182
Msa0999430	MtrunA17_Chr7g0260331	39.655	232	129	4	11	234	49	277	4.93e-51	173
Msa0999430	MtrunA17_Chr1g0204741	42.326	215	98	4	11	210	40	243	1.42e-50	171
Msa0999430	MtrunA17_Chr4g0071051	44.041	193	100	2	11	202	101	286	4.73e-45	159
Msa0999430	MtrunA17_Chr8g0392001	45.918	196	98	3	9	196	20	215	5.07e-45	158
Msa0999430	MtrunA17_Chr8g0379701	40.887	203	111	4	9	208	10	206	1.80e-43	154
Msa0999430	MtrunA17_Chr6g0467041	41.589	214	111	5	9	214	20	227	2.55e-43	152

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0999430	AT3G24520.1	59.906	212	59	3	8	215	14	203	9.82e-81	249
Msa0999430	AT4G17750.1	46.694	242	106	5	6	229	47	283	5.56e-59	198
Msa0999430	AT1G32330.2	49.239	197	90	2	6	192	32	228	8.92e-57	192
Msa0999430	AT1G32330.1	49.239	197	90	2	6	192	32	228	9.91e-57	192
Msa0999430	AT2G26150.1	44.878	205	108	3	11	210	44	248	3.41e-53	179
Msa0999430	AT2G26150.4	44.878	205	108	3	11	210	44	248	3.41e-53	179
Msa0999430	AT3G22830.1	37.778	270	133	6	11	268	61	307	2.17e-52	179
Msa0999430	AT3G02990.1	44.712	208	107	4	9	208	21	228	8.89e-51	176
Msa0999430	AT5G16820.1	44.340	212	108	4	7	208	23	234	2.08e-49	172
Msa0999430	AT5G16820.2	44.340	212	108	4	7	208	23	234	2.08e-49	172

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Find 90 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGGATCCGGATAGAT+GGG	0.516894	6_4:+81391658	Msa0999430:CDS
AAATTTCAATGAAATGACGG+CGG	0.731573	6_4:+81392420	Msa0999430:CDS
AAGAAACATGTTGGTGGTAT+TGG	0.321921	6_4:+81391740	Msa0999430:CDS
AATTTAGAAGAAGAGCTTCA+AGG	0.492990	6_4:+81391893	Msa0999430:CDS
ACACAGATAACCTTATCAAG+TGG	0.597268	6_4:+81391193	Msa0999430:CDS
ACAGATAACCTTATCAAGTG+GGG	0.700643	6_4:+81391195	Msa0999430:CDS
ACCGGAGCCACCTCCTGCGG+CGG	0.521738	6_4:-81392250	None:intergenic
ACGACGGTTGATATCTCCGG+TGG	0.680566	6_4:+81392054	Msa0999430:CDS
ACGATGAAACTGTTGTTAGA+TGG	0.404226	6_4:-81391219	None:intergenic
ACGGTTGCCGGTGTTGTCTC+CGG	0.466930	6_4:+81392336	Msa0999430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGTATGTCTTCATAATGAA+CGG	-	chr6_4:81391156-81391175	None:intergenic	30.0%
TTGATAAGGTTATCTGTGTT+TGG	-	chr6_4:81391192-81391211	None:intergenic	30.0%
AGAGAGTGAGAAAATTCTAA+AGG	-	chr6_4:81391252-81391271	None:intergenic	30.0%
TTGTTGTGTTTGAAGAAAAG+AGG	-	chr6_4:81391279-81391298	None:intergenic	30.0%
ATAGTTAGGAAGAAACATGT+TGG	+	chr6_4:81391731-81391750	Msa0999430:CDS	30.0%
TTTAGAAGAAGATGAAGCTA+TGG	+	chr6_4:81391835-81391854	Msa0999430:CDS	30.0%
AATTTAGAAGAAGAGCTTCA+AGG	+	chr6_4:81391893-81391912	Msa0999430:CDS	30.0%
GAAGTGATAGTAGTAAAAAG+GGG	+	chr6_4:81392389-81392408	Msa0999430:CDS	30.0%
AAGTGATAGTAGTAAAAAGG+GGG	+	chr6_4:81392390-81392409	Msa0999430:CDS	30.0%
CGGAAATTTCAATGAAATGA+CGG	+	chr6_4:81392417-81392436	Msa0999430:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	81391123	81392495	81391123	ID=Msa0999430;Name=Msa0999430
chr6_4	mRNA	81391123	81392495	81391123	ID=Msa0999430-mRNA-1;Parent=Msa0999430;Name=Msa0999430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|0\|0\|1\|1\|1\|2\|0\|351
chr6_4	exon	81391123	81391332	81391123	ID=Msa0999430-mRNA-1:exon:15558;Parent=Msa0999430-mRNA-1
chr6_4	exon	81391650	81392495	81391650	ID=Msa0999430-mRNA-1:exon:15559;Parent=Msa0999430-mRNA-1
chr6_4	CDS	81391123	81391332	81391123	ID=Msa0999430-mRNA-1:cds;Parent=Msa0999430-mRNA-1
chr6_4	CDS	81391650	81392495	81391650	ID=Msa0999430-mRNA-1:cds;Parent=Msa0999430-mRNA-1

Gene Sequence

>Msa0999430

ATGGGAATGGAGCAAAACAACGTGATTGCACCGTTCATTATGAAGACATACCACATGGTTAATGAACCAAACACAGATAACCTTATCAAGTGGGGCCCATCTAACAACAGTTTCATCGTTCTTGATCCTTTAGAATTTTCTCACTCTCTTCTTCCTCTTTTCTTCAAACACAACAATTTCTCAAGCTTTGTTCGTCAACTCAACACTTATGGTTTCAGAAAAGTGGATCCGGATAGATGGGAATTTGCGAATGAATGGTTTCTAAGAGGACAAAAACATTTGTTGAAGAACATAGTTAGGAAGAAACATGTTGGTGGTATTGGAAATGGAAGGAATAATAATAATAATAATTATTATAACAATAATAATTATTTTAATGAGGTGAAGTTTGAAGATTTAGAAGAAGATGAAGCTATGGTGATGGAAATAGCAAAGTTGAAAGAAGAACAAAGAAATTTAGAAGAAGAGCTTCAAGGAATGAACAAAAGATTAGAAACAACTGAGAAAAGACCACAACAAATGATGGCTTTTTTATGTAATGTCGTTGAAGATCCTGAAATTTTGTCTAGAATACTCAATGAAAGGGAGAGTAAACAATTTGTTGATAAGAAGAAACGACGGTTGATATCTCCGGTGGCTAAGGCGGCTGTGACGGCGGCAACGACGTCAACTTCCTCGGAGTCATCGGGCATGGCGGTGGCAAATTCGGTGAAGATGGAGTTTGAGGAAGATGAGTTAACGGTTGGTAATAATATGATGTCGTCGTCGTCGCCGGAGGTTGGTTTTGAAATTGATTATTTGCACCGTGCGCCGCCGCAGGAGGTGGCTCCGGTGGGTGGTTGGTGGGGGCAGATGGAGTTTGGACAGATGGGTTATAGGCGTGATTGTGAAGTTCCACGGTTGCCGGTGTTGTCTCCGGCGCCGGCGCCGATGATGTGGGGTGAGAATAGAAGTGATAGTAGTAAAAAGGGGGGTGTCGGAAATTTCAATGAAATGACGGCGGAAAATAGTAAGTTGCCACCTTATCCTTTTTCGTTGTTAGAAGGTGGCTTTTAG

Protein sequence

>Msa0999430

MGMEQNNVIAPFIMKTYHMVNEPNTDNLIKWGPSNNSFIVLDPLEFSHSLLPLFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEWFLRGQKHLLKNIVRKKHVGGIGNGRNNNNNNYYNNNNYFNEVKFEDLEEDEAMVMEIAKLKEEQRNLEEELQGMNKRLETTEKRPQQMMAFLCNVVEDPEILSRILNERESKQFVDKKKRRLISPVAKAAVTAATTSTSSESSGMAVANSVKMEFEEDELTVGNNMMSSSSPEVGFEIDYLHRAPPQEVAPVGGWWGQMEFGQMGYRRDCEVPRLPVLSPAPAPMMWGENRSDSSKKGGVGNFNEMTAENSKLPPYPFSLLEGGF*