AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0675410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675410 MtrunA17_Chr4g0018731 78.846 104 16 2 27 126 71 172 5.57e-27 99.4
Msa0675410 MtrunA17_Chr1g0168301 81.897 116 17 1 27 138 210 325 3.21e-19 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGTGTTTAAGACTAAC+AGG 0.286414 4_4:-24051750 Msa0675410:CDS
ATCATCAACTGACAGTGATT+CGG 0.382705 4_4:-24051512 Msa0675410:CDS
AAAGGAGGAGGAGGGAGCAC+AGG 0.386258 4_4:-24051699 Msa0675410:CDS
TCATCAACTGACAGTGATTC+GGG 0.386672 4_4:-24051511 Msa0675410:CDS
GATTCGGGTGATGGATATAA+TGG 0.404972 4_4:-24051496 Msa0675410:CDS
ACCATGGAGATGATGATTAG+AGG 0.412561 4_4:-24051423 None:intergenic
TGGATATAATGGTTCAAGTA+AGG 0.433386 4_4:-24051485 Msa0675410:CDS
CTATATTCATCACTATCATC+TGG 0.440092 4_4:+24051655 None:intergenic
GCTAGTGATTCAGATGGTTC+CGG 0.468678 4_4:-24051724 Msa0675410:CDS
ACTGACAGTGATTCGGGTGA+TGG 0.486358 4_4:-24051505 Msa0675410:CDS
CTCCCTCCTCCTCCTTTCAC+CGG 0.487250 4_4:+24051705 None:intergenic
GTAGGAAAGAGAAGAGGAGA+AGG 0.494224 4_4:-24051615 Msa0675410:CDS
ACAAAGATGCTGACCGAAAG+AGG 0.498522 4_4:-24051796 Msa0675410:CDS
ATTCAGATGGTTCCGGTGAA+AGG 0.502557 4_4:-24051717 Msa0675410:CDS
TCCTCTAATCATCATCTCCA+TGG 0.523954 4_4:+24051422 None:intergenic
GTTCCGGTGAAAGGAGGAGG+AGG 0.525308 4_4:-24051708 Msa0675410:CDS
ATGAATATAGACACAAGAAA+AGG 0.532593 4_4:-24051642 Msa0675410:CDS
GGATATAATGGTTCAAGTAA+GGG 0.549938 4_4:-24051484 Msa0675410:CDS
ATGGTTCCGGTGAAAGGAGG+AGG 0.558346 4_4:-24051711 Msa0675410:CDS
TAATGGTTCAAGTAAGGGTA+GGG 0.560844 4_4:-24051479 Msa0675410:CDS
GACACAAGAAAAGGAAGAGT+AGG 0.567421 4_4:-24051633 Msa0675410:CDS
TTCTGATTCTGAAACATCTG+AGG 0.573778 4_4:-24051575 Msa0675410:CDS
AGAGAGGTAGAGAAAAGAGA+AGG 0.579127 4_4:-24051769 Msa0675410:CDS
ATAATGGTTCAAGTAAGGGT+AGG 0.582111 4_4:-24051480 Msa0675410:CDS
GGAAGAGTAGGAAAGAGAAG+AGG 0.590104 4_4:-24051621 Msa0675410:CDS
GGGAGCACAGGAGAAGCATG+AGG 0.590249 4_4:-24051687 Msa0675410:CDS
AAGAGGAGGAGACGCCACCA+TGG 0.594257 4_4:-24051439 Msa0675410:CDS
CAGATGGTTCCGGTGAAAGG+AGG 0.617536 4_4:-24051714 Msa0675410:CDS
CATCTGAGGATTCTACTTCG+CGG 0.617958 4_4:-24051561 Msa0675410:CDS
AGGGAAGCTAGTGATTCAGA+TGG 0.649064 4_4:-24051730 Msa0675410:CDS
GGAGCACAGGAGAAGCATGA+GGG 0.656858 4_4:-24051686 Msa0675410:CDS
TTCCGGTGAAAGGAGGAGGA+GGG 0.666380 4_4:-24051707 Msa0675410:CDS
AGGAGTGTTTAAGACTAACA+GGG 0.683554 4_4:-24051749 Msa0675410:CDS
TCTAATCATCATCTCCATGG+TGG 0.691101 4_4:+24051425 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAAAAAATCAGAAAAG+AGG - chr4_4:24051790-24051809 Msa0675410:CDS 20.0%
! ATGAATATAGACACAAGAAA+AGG - chr4_4:24051604-24051623 Msa0675410:CDS 25.0%
CTATATTCATCACTATCATC+TGG + chr4_4:24051594-24051613 None:intergenic 30.0%
TGGATATAATGGTTCAAGTA+AGG - chr4_4:24051761-24051780 Msa0675410:CDS 30.0%
GGATATAATGGTTCAAGTAA+GGG - chr4_4:24051762-24051781 Msa0675410:CDS 30.0%
TCAAAAAATCAGAAAAGAGG+AGG - chr4_4:24051793-24051812 Msa0675410:CDS 30.0%
! TACCTCTCTTTTTCCTCTTT+CGG + chr4_4:24051466-24051485 None:intergenic 35.0%
!! AAGGAGTGTTTAAGACTAAC+AGG - chr4_4:24051496-24051515 Msa0675410:CDS 35.0%
!! AGGAGTGTTTAAGACTAACA+GGG - chr4_4:24051497-24051516 Msa0675410:CDS 35.0%
!! TTCTGATTCTGAAACATCTG+AGG - chr4_4:24051671-24051690 Msa0675410:CDS 35.0%
ATCATCAACTGACAGTGATT+CGG - chr4_4:24051734-24051753 Msa0675410:CDS 35.0%
ATAATGGTTCAAGTAAGGGT+AGG - chr4_4:24051766-24051785 Msa0675410:CDS 35.0%
TAATGGTTCAAGTAAGGGTA+GGG - chr4_4:24051767-24051786 Msa0675410:CDS 35.0%
AGAGAGGTAGAGAAAAGAGA+AGG - chr4_4:24051477-24051496 Msa0675410:CDS 40.0%
GACACAAGAAAAGGAAGAGT+AGG - chr4_4:24051613-24051632 Msa0675410:CDS 40.0%
TCATCAACTGACAGTGATTC+GGG - chr4_4:24051735-24051754 Msa0675410:CDS 40.0%
!! GATTCGGGTGATGGATATAA+TGG - chr4_4:24051750-24051769 Msa0675410:CDS 40.0%
ACAAAGATGCTGACCGAAAG+AGG - chr4_4:24051450-24051469 Msa0675410:CDS 45.0%
GACCGAAAGAGGAAAAAGAG+AGG - chr4_4:24051461-24051480 Msa0675410:CDS 45.0%
AGGGAAGCTAGTGATTCAGA+TGG - chr4_4:24051516-24051535 Msa0675410:CDS 45.0%
GCTAGTGATTCAGATGGTTC+CGG - chr4_4:24051522-24051541 Msa0675410:CDS 45.0%
ATTCAGATGGTTCCGGTGAA+AGG - chr4_4:24051529-24051548 Msa0675410:CDS 45.0%
GGAAGAGTAGGAAAGAGAAG+AGG - chr4_4:24051625-24051644 Msa0675410:CDS 45.0%
GTAGGAAAGAGAAGAGGAGA+AGG - chr4_4:24051631-24051650 Msa0675410:CDS 45.0%
CATCTGAGGATTCTACTTCG+CGG - chr4_4:24051685-24051704 Msa0675410:CDS 45.0%
! ACTGACAGTGATTCGGGTGA+TGG - chr4_4:24051741-24051760 Msa0675410:CDS 50.0%
CAGATGGTTCCGGTGAAAGG+AGG - chr4_4:24051532-24051551 Msa0675410:CDS 55.0%
ATGGTTCCGGTGAAAGGAGG+AGG - chr4_4:24051535-24051554 Msa0675410:CDS 55.0%
TTCCGGTGAAAGGAGGAGGA+GGG - chr4_4:24051539-24051558 Msa0675410:CDS 55.0%
! GGAGCACAGGAGAAGCATGA+GGG - chr4_4:24051560-24051579 Msa0675410:CDS 55.0%
GTTCCGGTGAAAGGAGGAGG+AGG - chr4_4:24051538-24051557 Msa0675410:CDS 60.0%
CTCCCTCCTCCTCCTTTCAC+CGG + chr4_4:24051544-24051563 None:intergenic 60.0%
! AAAGGAGGAGGAGGGAGCAC+AGG - chr4_4:24051547-24051566 Msa0675410:CDS 60.0%
! GGGAGCACAGGAGAAGCATG+AGG - chr4_4:24051559-24051578 Msa0675410:CDS 60.0%
AAGAGGAGGAGACGCCACCA+TGG - chr4_4:24051807-24051826 Msa0675410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 24051426 24051842 24051426 ID=Msa0675410;Name=Msa0675410
chr4_4 mRNA 24051426 24051842 24051426 ID=Msa0675410-mRNA-1;Parent=Msa0675410;Name=Msa0675410-mRNA-1;_AED=0.39;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|138
chr4_4 exon 24051426 24051842 24051426 ID=Msa0675410-mRNA-1:exon:6708;Parent=Msa0675410-mRNA-1
chr4_4 CDS 24051426 24051842 24051426 ID=Msa0675410-mRNA-1:cds;Parent=Msa0675410-mRNA-1
Gene Sequence

>Msa0675410

ATGACTAAGATGATGACGAGTCAGACAAAGATGCTGACCGAAAGAGGAAAAAGAGAGGTAGAGAAAAGAGAAGGAGTGTTTAAGACTAACAGGGAAGCTAGTGATTCAGATGGTTCCGGTGAAAGGAGGAGGAGGGAGCACAGGAGAAGCATGAGGGACGAGTCTCCAGATGATAGTGATGAATATAGACACAAGAAAAGGAAGAGTAGGAAAGAGAAGAGGAGAAGGAGAAGTCGTCTTTCAGATTCTGATTCTGAAACATCTGAGGATTCTACTTCGCGGCACAAGCGAAATAGCAAGAGAACTTCATCATCAACTGACAGTGATTCGGGTGATGGATATAATGGTTCAAGTAAGGGTAGGGATATCAAAAAATCAGAAAAGAGGAGGAGACGCCACCATGGAGATGATGATTAG

Protein sequence

>Msa0675410

MTKMMTSQTKMLTERGKREVEKREGVFKTNREASDSDGSGERRRREHRRSMRDESPDDSDEYRHKKRKSRKEKRRRRSRLSDSDSETSEDSTSRHKRNSKRTSSSTDSDSGDGYNGSSKGRDIKKSEKRRRRHHGDDD*