AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541360 MtrunA17_Chr4g0018731 66.667 177 42 5 1 165 1 172 2.61e-40 134
Msa0541360 MtrunA17_Chr1g0168301 81.897 116 17 1 66 177 210 325 5.07e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGTGTTTAAGACTAAC+AGG 0.286414 4_1:+25179381 Msa0541360:CDS
ATCATCAACTGACAGTGATT+CGG 0.382705 4_1:+25179619 Msa0541360:CDS
AAAGGAGGAGGAGGGAGCAC+AGG 0.386258 4_1:+25179432 Msa0541360:CDS
TCATCAACTGACAGTGATTC+GGG 0.386672 4_1:+25179620 Msa0541360:CDS
GATTCGGGTGATGGATATAA+TGG 0.404972 4_1:+25179635 Msa0541360:CDS
ACCATGGAGATGATGATTAG+AGG 0.412561 4_1:+25179708 Msa0541360:CDS
TGGATATAATGGTTCAAGTA+AGG 0.433386 4_1:+25179646 Msa0541360:CDS
CTATATTCATCACTATCATC+TGG 0.440092 4_1:-25179476 None:intergenic
GAGAGGTTCTTATAGAAAGA+AGG 0.453802 4_1:+25179288 Msa0541360:intron
TTGAGAGAGCTATTGCTAAA+GGG 0.463888 4_1:+25179250 Msa0541360:CDS
GCTAGTGATTCAGATGGTTC+CGG 0.468678 4_1:+25179407 Msa0541360:CDS
ACTGACAGTGATTCGGGTGA+TGG 0.486358 4_1:+25179626 Msa0541360:CDS
CTCCCTCCTCCTCCTTTCAC+CGG 0.487250 4_1:-25179426 None:intergenic
GTAGGAAAGAGAAGAGGAGA+AGG 0.494224 4_1:+25179516 Msa0541360:CDS
ACAAAGATGCTGACCGAAAG+AGG 0.498522 4_1:+25179335 Msa0541360:CDS
ATTCAGATGGTTCCGGTGAA+AGG 0.502557 4_1:+25179414 Msa0541360:CDS
GAGCTCAGAAGAGGAGGAAG+AGG 0.513498 4_1:+25179191 Msa0541360:CDS
ATTGAGAGAGCTATTGCTAA+AGG 0.513599 4_1:+25179249 Msa0541360:CDS
TCCTCTAATCATCATCTCCA+TGG 0.523954 4_1:-25179709 None:intergenic
GTTCCGGTGAAAGGAGGAGG+AGG 0.525308 4_1:+25179423 Msa0541360:CDS
ATGAATATAGACACAAGAAA+AGG 0.532593 4_1:+25179489 Msa0541360:CDS
GGATATAATGGTTCAAGTAA+GGG 0.549938 4_1:+25179647 Msa0541360:CDS
ATGGTTCCGGTGAAAGGAGG+AGG 0.558346 4_1:+25179420 Msa0541360:CDS
TAATGGTTCAAGTAAGGGTA+GGG 0.560844 4_1:+25179652 Msa0541360:CDS
TGTTGAGAGCTCAGAAGAGG+AGG 0.561377 4_1:+25179185 Msa0541360:CDS
GACACAAGAAAAGGAAGAGT+AGG 0.567421 4_1:+25179498 Msa0541360:CDS
TTCTGATTCTGAAACATCTG+AGG 0.573778 4_1:+25179556 Msa0541360:CDS
GGGGAAGTTAGTGAGAAGAG+AGG 0.575217 4_1:+25179271 Msa0541360:CDS
AGAGAGGTAGAGAAAAGAGA+AGG 0.579127 4_1:+25179362 Msa0541360:CDS
ATAATGGTTCAAGTAAGGGT+AGG 0.582111 4_1:+25179651 Msa0541360:CDS
TGAGAGAGCTATTGCTAAAG+GGG 0.588008 4_1:+25179251 Msa0541360:CDS
GGAAGAGTAGGAAAGAGAAG+AGG 0.590104 4_1:+25179510 Msa0541360:CDS
GGGAGCACAGGAGAAGCATG+AGG 0.590249 4_1:+25179444 Msa0541360:CDS
CAATGTTGAGAGCTCAGAAG+AGG 0.592360 4_1:+25179182 None:intergenic
AAGAGGAGGAGACGCCACCA+TGG 0.594257 4_1:+25179692 Msa0541360:CDS
CAGATGGTTCCGGTGAAAGG+AGG 0.617536 4_1:+25179417 Msa0541360:CDS
CATCTGAGGATTCTACTTCG+CGG 0.617958 4_1:+25179570 Msa0541360:CDS
AGGGAAGCTAGTGATTCAGA+TGG 0.649064 4_1:+25179401 Msa0541360:CDS
GGAGCACAGGAGAAGCATGA+GGG 0.656858 4_1:+25179445 Msa0541360:CDS
TTCCGGTGAAAGGAGGAGGA+GGG 0.666380 4_1:+25179424 Msa0541360:CDS
AGGAGTGTTTAAGACTAACA+GGG 0.683554 4_1:+25179382 Msa0541360:CDS
TCTAATCATCATCTCCATGG+TGG 0.691101 4_1:-25179706 None:intergenic
GAGAGAGCTATTGCTAAAGG+GGG 0.696345 4_1:+25179252 Msa0541360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAAAAAATCAGAAAAG+AGG + chr4_1:25179675-25179694 Msa0541360:CDS 20.0%
! ATGAATATAGACACAAGAAA+AGG + chr4_1:25179489-25179508 Msa0541360:CDS 25.0%
CTATATTCATCACTATCATC+TGG - chr4_1:25179479-25179498 None:intergenic 30.0%
TGGATATAATGGTTCAAGTA+AGG + chr4_1:25179646-25179665 Msa0541360:CDS 30.0%
GGATATAATGGTTCAAGTAA+GGG + chr4_1:25179647-25179666 Msa0541360:CDS 30.0%
TCAAAAAATCAGAAAAGAGG+AGG + chr4_1:25179678-25179697 Msa0541360:CDS 30.0%
ATTGAGAGAGCTATTGCTAA+AGG + chr4_1:25179249-25179268 Msa0541360:CDS 35.0%
TTGAGAGAGCTATTGCTAAA+GGG + chr4_1:25179250-25179269 Msa0541360:CDS 35.0%
GAGAGGTTCTTATAGAAAGA+AGG + chr4_1:25179288-25179307 Msa0541360:intron 35.0%
! TACCTCTCTTTTTCCTCTTT+CGG - chr4_1:25179351-25179370 None:intergenic 35.0%
!! AAGGAGTGTTTAAGACTAAC+AGG + chr4_1:25179381-25179400 Msa0541360:CDS 35.0%
!! AGGAGTGTTTAAGACTAACA+GGG + chr4_1:25179382-25179401 Msa0541360:CDS 35.0%
!! TTCTGATTCTGAAACATCTG+AGG + chr4_1:25179556-25179575 Msa0541360:CDS 35.0%
ATCATCAACTGACAGTGATT+CGG + chr4_1:25179619-25179638 Msa0541360:CDS 35.0%
ATAATGGTTCAAGTAAGGGT+AGG + chr4_1:25179651-25179670 Msa0541360:CDS 35.0%
TAATGGTTCAAGTAAGGGTA+GGG + chr4_1:25179652-25179671 Msa0541360:CDS 35.0%
TGAGAGAGCTATTGCTAAAG+GGG + chr4_1:25179251-25179270 Msa0541360:CDS 40.0%
AGAGAGGTAGAGAAAAGAGA+AGG + chr4_1:25179362-25179381 Msa0541360:CDS 40.0%
GACACAAGAAAAGGAAGAGT+AGG + chr4_1:25179498-25179517 Msa0541360:CDS 40.0%
TCATCAACTGACAGTGATTC+GGG + chr4_1:25179620-25179639 Msa0541360:CDS 40.0%
!! GATTCGGGTGATGGATATAA+TGG + chr4_1:25179635-25179654 Msa0541360:CDS 40.0%
GAGAGAGCTATTGCTAAAGG+GGG + chr4_1:25179252-25179271 Msa0541360:CDS 45.0%
ACAAAGATGCTGACCGAAAG+AGG + chr4_1:25179335-25179354 Msa0541360:CDS 45.0%
GACCGAAAGAGGAAAAAGAG+AGG + chr4_1:25179346-25179365 Msa0541360:CDS 45.0%
AGGGAAGCTAGTGATTCAGA+TGG + chr4_1:25179401-25179420 Msa0541360:CDS 45.0%
GCTAGTGATTCAGATGGTTC+CGG + chr4_1:25179407-25179426 Msa0541360:CDS 45.0%
ATTCAGATGGTTCCGGTGAA+AGG + chr4_1:25179414-25179433 Msa0541360:CDS 45.0%
GGAAGAGTAGGAAAGAGAAG+AGG + chr4_1:25179510-25179529 Msa0541360:CDS 45.0%
GTAGGAAAGAGAAGAGGAGA+AGG + chr4_1:25179516-25179535 Msa0541360:CDS 45.0%
CATCTGAGGATTCTACTTCG+CGG + chr4_1:25179570-25179589 Msa0541360:CDS 45.0%
TGTTGAGAGCTCAGAAGAGG+AGG + chr4_1:25179185-25179204 Msa0541360:CDS 50.0%
GGGGAAGTTAGTGAGAAGAG+AGG + chr4_1:25179271-25179290 Msa0541360:CDS 50.0%
! ACTGACAGTGATTCGGGTGA+TGG + chr4_1:25179626-25179645 Msa0541360:CDS 50.0%
GAGCTCAGAAGAGGAGGAAG+AGG + chr4_1:25179191-25179210 Msa0541360:CDS 55.0%
CAGATGGTTCCGGTGAAAGG+AGG + chr4_1:25179417-25179436 Msa0541360:CDS 55.0%
ATGGTTCCGGTGAAAGGAGG+AGG + chr4_1:25179420-25179439 Msa0541360:CDS 55.0%
TTCCGGTGAAAGGAGGAGGA+GGG + chr4_1:25179424-25179443 Msa0541360:CDS 55.0%
! GGAGCACAGGAGAAGCATGA+GGG + chr4_1:25179445-25179464 Msa0541360:CDS 55.0%
GTTCCGGTGAAAGGAGGAGG+AGG + chr4_1:25179423-25179442 Msa0541360:CDS 60.0%
CTCCCTCCTCCTCCTTTCAC+CGG - chr4_1:25179429-25179448 None:intergenic 60.0%
! AAAGGAGGAGGAGGGAGCAC+AGG + chr4_1:25179432-25179451 Msa0541360:CDS 60.0%
! GGGAGCACAGGAGAAGCATG+AGG + chr4_1:25179444-25179463 Msa0541360:CDS 60.0%
AAGAGGAGGAGACGCCACCA+TGG + chr4_1:25179692-25179711 Msa0541360:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25179184 25179727 25179184 ID=Msa0541360;Name=Msa0541360
chr4_1 mRNA 25179184 25179727 25179184 ID=Msa0541360-mRNA-1;Parent=Msa0541360;Name=Msa0541360-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|0|0|2|0|177
chr4_1 exon 25179184 25179292 25179184 ID=Msa0541360-mRNA-1:exon:6787;Parent=Msa0541360-mRNA-1
chr4_1 exon 25179303 25179727 25179303 ID=Msa0541360-mRNA-1:exon:6788;Parent=Msa0541360-mRNA-1
chr4_1 CDS 25179184 25179292 25179184 ID=Msa0541360-mRNA-1:cds;Parent=Msa0541360-mRNA-1
chr4_1 CDS 25179303 25179727 25179303 ID=Msa0541360-mRNA-1:cds;Parent=Msa0541360-mRNA-1
Gene Sequence

>Msa0541360

ATGTTGAGAGCTCAGAAGAGGAGGAAGAGGATAGTGAGAGTTCAGATTCTGAGATTGATTCAGAGATTGAGAGAGCTATTGCTAAAGGGGGAAGTTAGTGAGAAGAGAGAAAGAAGGATGACTAAGATGATGACGAGTCAGACAAAGATGCTGACCGAAAGAGGAAAAAGAGAGGTAGAGAAAAGAGAAGGAGTGTTTAAGACTAACAGGGAAGCTAGTGATTCAGATGGTTCCGGTGAAAGGAGGAGGAGGGAGCACAGGAGAAGCATGAGGGACGAGTCTCCAGATGATAGTGATGAATATAGACACAAGAAAAGGAAGAGTAGGAAAGAGAAGAGGAGAAGGAGAAGTCGTCTTTCAGATTCTGATTCTGAAACATCTGAGGATTCTACTTCGCGGCACAAGCGAAATAGCAAGAGAACTTCATCATCAACTGACAGTGATTCGGGTGATGGATATAATGGTTCAAGTAAGGGTAGGGATATCAAAAAATCAGAAAAGAGGAGGAGACGCCACCATGGAGATGATGATTAG

Protein sequence

>Msa0541360

MLRAQKRRKRIVRVQILRLIQRLRELLLKGEVSEKRERRMTKMMTSQTKMLTERGKREVEKREGVFKTNREASDSDGSGERRRREHRRSMRDESPDDSDEYRHKKRKSRKEKRRRRSRLSDSDSETSEDSTSRHKRNSKRTSSSTDSDSGDGYNGSSKGRDIKKSEKRRRRHHGDDD*