AlfalfaGEDB | Msa1253660

M. sativa cultivar zhongmu-4 / Msa1253660

KEGG: K08472
GO: GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009856 GO:0010483 GO:0012505 GO:0016020 GO:0022414 GO:0032501 GO:0044424 GO:0044464 GO:0044703 GO:0044706 GO:0050896 GO:0051703 GO:0051704 GO:0071944
PFAM: PF03094.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253660	XP_003629098.2	93.027	588	41	0	1	588	1	588	0.0	1129

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253660	sp\|O22757\|MLO8_ARATH	61.458	576	196	8	27	588	28	591	0.0	704

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015670	Msa1253660	0.803826	2.895610e-49	-8.615850e-47
Msa0036540	Msa1253660	0.802738	4.865086e-49	-8.615850e-47
Msa0043650	Msa1253660	0.800924	1.146996e-48	-8.615850e-47
Msa0045550	Msa1253660	0.857378	1.700413e-62	-8.615850e-47
Msa0055070	Msa1253660	0.808311	3.296593e-50	-8.615850e-47
Msa0058310	Msa1253660	0.800655	1.301670e-48	-8.615850e-47
Msa0071480	Msa1253660	0.802722	4.903096e-49	-8.615850e-47
Msa0092410	Msa1253660	0.862821	3.862540e-64	-8.615850e-47
Msa0098290	Msa1253660	0.805329	1.406833e-49	-8.615850e-47
Msa0101020	Msa1253660	0.810630	1.047695e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253660	MtrunA17_Chr8g0370181	93.027	588	41	0	1	588	1	588	0.0	1129
Msa1253660	MtrunA17_Chr5g0407381	69.464	560	149	10	8	560	3	547	0.0	777
Msa1253660	MtrunA17_Chr3g0144201	56.513	522	170	3	37	502	10	530	0.0	566
Msa1253660	MtrunA17_Chr3g0144171	48.467	522	247	7	36	542	7	521	1.38e-171	498
Msa1253660	MtrunA17_Chr6g0462051	47.269	531	254	8	31	540	2	527	1.26e-168	491
Msa1253660	MtrunA17_Chr2g0326301	46.230	504	247	6	37	525	7	501	8.48e-154	454
Msa1253660	MtrunA17_Chr8g0337941	43.149	489	255	5	38	503	12	500	4.07e-133	399
Msa1253660	MtrunA17_Chr3g0145831	43.875	449	230	6	40	471	14	457	1.69e-127	384
Msa1253660	MtrunA17_Chr4g0040611	38.122	543	306	8	35	569	4	524	1.16e-121	371
Msa1253660	MtrunA17_Chr2g0317801	39.212	533	285	14	31	545	2	513	5.62e-118	360

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253660	AT2G17480.1	61.458	576	196	8	27	588	28	591	0.0	704
Msa1253660	AT5G65970.1	63.551	535	178	5	9	537	4	527	0.0	667
Msa1253660	AT2G17430.1	60.229	523	197	4	13	531	7	522	0.0	641
Msa1253660	AT2G33670.1	62.443	442	159	4	38	478	15	450	0.0	578
Msa1253660	AT1G42560.1	60.762	446	170	3	38	479	14	458	0.0	550
Msa1253660	AT5G65970.2	65.160	376	119	3	9	383	4	368	0.0	521
Msa1253660	AT1G61560.1	49.901	503	231	6	38	523	8	506	8.65e-171	498
Msa1253660	AT1G61560.4	48.509	503	238	6	38	523	8	506	1.35e-161	474
Msa1253660	AT2G39200.1	43.517	563	282	7	38	584	6	548	2.70e-161	473
Msa1253660	AT1G11310.1	48.096	499	230	7	38	512	8	501	3.40e-159	468

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Find 137 sgRNAs with CRISPR-Local

Find 394 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATATCAAAAGATCCT+TGG	0.464347	8_2:-51276880	Msa1253660:CDS
AAAATTGAATACCTGGAACA+AGG	0.654833	8_2:+51275934	None:intergenic
AAACAACGAATGATGCCCAA+AGG	0.683770	8_2:+51276719	None:intergenic
AAAGACATGCTGTGGTCCAA+GGG	0.583506	8_2:-51276033	Msa1253660:CDS
AAAGTCGAGTGTGCGAACCA+GGG	0.675466	8_2:-51274298	Msa1253660:CDS
AAATTTCTTGCTTCTGATTA+TGG	0.198695	8_2:+51280468	None:intergenic
AACAAGAGCATATAATGGAA+GGG	0.604802	8_2:+51274844	None:intergenic
AACAGAATACTTACAATGAC+AGG	0.501258	8_2:+51276549	None:intergenic
AACATATTTCTTGTGGATAT+GGG	0.388150	8_2:-51275508	Msa1253660:intron
AACCCATATCATACATATTA+TGG	0.263412	8_2:-51280355	Msa1253660:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGAAGTTATGTATATTAGG+TGG	+	chr8_2:51273813-51273832	None:intergenic	30.0%
TTGTACTTCGACATAAACAT+AGG	+	chr8_2:51274055-51274074	None:intergenic	30.0%
TTATGTCGAAGTACAAACAA+AGG	-	chr8_2:51274059-51274078	Msa1253660:CDS	30.0%
AAGTCTTCACAAAATTGGAA+CGG	-	chr8_2:51274246-51274265	Msa1253660:CDS	30.0%
AGATTCATAGTTGTGAACTT+AGG	-	chr8_2:51274632-51274651	Msa1253660:intron	30.0%
GATTCATAGTTGTGAACTTA+GGG	-	chr8_2:51274633-51274652	Msa1253660:intron	30.0%
ATTCATAGTTGTGAACTTAG+GGG	-	chr8_2:51274634-51274653	Msa1253660:intron	30.0%
TCATAGAATTTCTTTCCAGT+TGG	-	chr8_2:51274781-51274800	Msa1253660:intron	30.0%
GTTAATTCCTATTATTGGTC+AGG	-	chr8_2:51275515-51275534	Msa1253660:CDS	30.0%
ATTGAAACCTGACCAATAAT+AGG	+	chr8_2:51275525-51275544	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	51273699	51280568	51273699	ID=Msa1253660;Name=Msa1253660
chr8_2	mRNA	51273699	51280568	51273699	ID=Msa1253660-mRNA-1;Parent=Msa1253660;Name=Msa1253660-mRNA-1;_AED=0.32;_eAED=0.32;_QI=297\|1\|1\|1\|0.87\|0.76\|17\|249\|588
chr8_2	exon	51273699	51273787	51273699	ID=Msa1253660-mRNA-1:exon:15738;Parent=Msa1253660-mRNA-1
chr8_2	exon	51273845	51274421	51273845	ID=Msa1253660-mRNA-1:exon:15737;Parent=Msa1253660-mRNA-1
chr8_2	exon	51274838	51274898	51274838	ID=Msa1253660-mRNA-1:exon:15736;Parent=Msa1253660-mRNA-1
chr8_2	exon	51275352	51275419	51275352	ID=Msa1253660-mRNA-1:exon:15735;Parent=Msa1253660-mRNA-1
chr8_2	exon	51275509	51275544	51275509	ID=Msa1253660-mRNA-1:exon:15734;Parent=Msa1253660-mRNA-1
chr8_2	exon	51275946	51276119	51275946	ID=Msa1253660-mRNA-1:exon:15733;Parent=Msa1253660-mRNA-1
chr8_2	exon	51276563	51276603	51276563	ID=Msa1253660-mRNA-1:exon:15732;Parent=Msa1253660-mRNA-1
chr8_2	exon	51276698	51276747	51276698	ID=Msa1253660-mRNA-1:exon:15731;Parent=Msa1253660-mRNA-1
chr8_2	exon	51276849	51276937	51276849	ID=Msa1253660-mRNA-1:exon:15730;Parent=Msa1253660-mRNA-1
chr8_2	exon	51277011	51277085	51277011	ID=Msa1253660-mRNA-1:exon:15729;Parent=Msa1253660-mRNA-1
chr8_2	exon	51277189	51277277	51277189	ID=Msa1253660-mRNA-1:exon:15728;Parent=Msa1253660-mRNA-1
chr8_2	exon	51277576	51277636	51277576	ID=Msa1253660-mRNA-1:exon:15727;Parent=Msa1253660-mRNA-1
chr8_2	exon	51278480	51278596	51278480	ID=Msa1253660-mRNA-1:exon:15726;Parent=Msa1253660-mRNA-1
chr8_2	exon	51279088	51279305	51279088	ID=Msa1253660-mRNA-1:exon:15725;Parent=Msa1253660-mRNA-1
chr8_2	exon	51279653	51279710	51279653	ID=Msa1253660-mRNA-1:exon:15724;Parent=Msa1253660-mRNA-1
chr8_2	exon	51280000	51280213	51280000	ID=Msa1253660-mRNA-1:exon:15723;Parent=Msa1253660-mRNA-1
chr8_2	exon	51280273	51280568	51280273	ID=Msa1253660-mRNA-1:exon:15722;Parent=Msa1253660-mRNA-1
chr8_2	five_prime_UTR	51280273	51280568	51280273	ID=Msa1253660-mRNA-1:five_prime_utr;Parent=Msa1253660-mRNA-1
chr8_2	five_prime_UTR	51280213	51280213	51280213	ID=Msa1253660-mRNA-1:five_prime_utr;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51280000	51280212	51280000	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51279653	51279710	51279653	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51279088	51279305	51279088	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51278480	51278596	51278480	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51277576	51277636	51277576	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51277189	51277277	51277189	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51277011	51277085	51277011	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51276849	51276937	51276849	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51276698	51276747	51276698	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51276563	51276603	51276563	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51275946	51276119	51275946	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51275509	51275544	51275509	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51275352	51275419	51275352	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51274838	51274898	51274838	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	CDS	51274005	51274421	51274005	ID=Msa1253660-mRNA-1:cds;Parent=Msa1253660-mRNA-1
chr8_2	three_prime_UTR	51273845	51274004	51273845	ID=Msa1253660-mRNA-1:three_prime_utr;Parent=Msa1253660-mRNA-1
chr8_2	three_prime_UTR	51273699	51273787	51273699	ID=Msa1253660-mRNA-1:three_prime_utr;Parent=Msa1253660-mRNA-1

Gene Sequence

>Msa1253660

ATGTTTATGTCGAAGTACAAACAAAGGTTGTTTCTGTGTCTGGTTTCATTTGCATGGCTGTACAATGCTAGTGTGGCTATGGCTTCTTCAAGTGAAGAAAGTGGAAGTTCTAAAGACCTTGATCAGACACCAACATGGGCTGTTGCTTGTGTTTGTACTGTTTTCATCTTGATTTCTATAATTCTTGAAAAAAGTCTTCACAAAATTGGAACGTGGCTGGGAGATAGGCACAAGAGGGCTTTGCTTGAAGCTCTGGAAAAAGTCAAAGCTGAATTGATGATTTTAGGTTTCATTTCATTGCTTTTGACGTTTGGGCAGAGCTACATTGTTAAAATATGCATCCCTGCCAATGCGGCAGACAAACTGCTGCCATGTCCATATAGAGCTTCCCGCGATGCAAAAAATGATGAAGAGGAACATCATAGGAAACTTCTCTATTATGAGCACAGATATTTATCAGAAGATGCTACCCCTTACAAATGCAAGGAGGGACATGAGCCACTTATATCTGTCAATGGATTGCACCAGTTACACATTCTCATATTCTTTTTAGCAGTCCTTCACGTGATATACAGTGCTGTCACAATGCTTCTCGGGAGACTAAAGATACGAGGATGGAAGGCATGGGAGGCAGAGACATCGTCTCACGGATATGAGTTCGCCAATGATCCTTCAAGATTCCGGCTTACACATGAAACATCATTTGTTAGACAACATGCCACTTTTTGGACAAAAATGCCAATCTTCTTCTATATAAGTTGCTTCTTTAGACAGTTCTATAGGTCTGTCGAGAAAGCTGACTACCTGACTCTGCGGAACGGATTTATCGCGGTCCACCTGTCACCAGGAAGTAAATTTAACTTTCAAAAATATATCAAAAGATCCTTGGAGGATGACTTCAAGGTGGTTGTCGGAGTCAGTCCTGTCCTTTGGGCATCATTCGTTGTTTTCCTTCTCCTAAATGTTGAAGGATGGCATGCTATGTTTTGGGCATCCTTAATTCCTGTCATTATAATTTTGGCTGTTGGAACAAAACTTCAAGCCACACTGTCAAAGATGGCTATTGAAATCACAGAAAGACATGCTGTGGTCCAAGGGATTCCCCTTGTTCAAGGCTCAGACAAATATTTTTGGTTTGGGAAGCCTCAGTTGCTTCTTCATCTTATCCATTTTGCCTTGTTCCAGAATGCGTTTCAGATAACATATTTCTTGTGGATATGGTATTCTTTCGGGCTGATAAATTGTTTCCATGCTGATTACAAGCTTGCCATAGTCAAAGTAGCTTTAGGGCTCGCGGTGCTAGTCCTCTGCAGCTACATGACCCTTCCATTATATGCTCTTGTTACTCAGATGGGATCAACAATGAAAAAATCAATATTTGATGATCAAACATCAAAGGCGTTAAAAAAATGGCACAATACTGTGAAAAAGAAGCATGGAGTGAAACTAGGAAAGTCGAGTGTGCGAACCAGGGACGGAAGCACCACCGATTCAACAGTGCACTCTATTAGTCCCACACTACACCGTTACAAAACTACCGGTCACTCAACTCGCTACGATAACGACCAAGATGATTACCAATCTGATATTGAATTGACTCCGACATCACCAACGTCAAACTTGCTAGCAGTAAGAGTTGATCATGTTGAGCAAGATGCAAAAGAAAATGAACACCAAGCCATCGTAGAAACCATTGAACAACAACAAACAGTTCTGCGTAGCTTGTCATTTGTCAAACCTGATCATCCGGAAAGAAGCGCGAATTAA

Protein sequence

>Msa1253660

MFMSKYKQRLFLCLVSFAWLYNASVAMASSSEESGSSKDLDQTPTWAVACVCTVFILISIILEKSLHKIGTWLGDRHKRALLEALEKVKAELMILGFISLLLTFGQSYIVKICIPANAADKLLPCPYRASRDAKNDEEEHHRKLLYYEHRYLSEDATPYKCKEGHEPLISVNGLHQLHILIFFLAVLHVIYSAVTMLLGRLKIRGWKAWEAETSSHGYEFANDPSRFRLTHETSFVRQHATFWTKMPIFFYISCFFRQFYRSVEKADYLTLRNGFIAVHLSPGSKFNFQKYIKRSLEDDFKVVVGVSPVLWASFVVFLLLNVEGWHAMFWASLIPVIIILAVGTKLQATLSKMAIEITERHAVVQGIPLVQGSDKYFWFGKPQLLLHLIHFALFQNAFQITYFLWIWYSFGLINCFHADYKLAIVKVALGLAVLVLCSYMTLPLYALVTQMGSTMKKSIFDDQTSKALKKWHNTVKKKHGVKLGKSSVRTRDGSTTDSTVHSISPTLHRYKTTGHSTRYDNDQDDYQSDIELTPTSPTSNLLAVRVDHVEQDAKENEHQAIVETIEQQQTVLRSLSFVKPDHPERSAN*