AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95020.t1 MTR_4g066050 94.163 257 15 0 1 257 1 257 1.14e-180 496
MS.gene95020.t1 MTR_4g070490 77.519 258 56 2 1 257 1 257 8.78e-140 393
MS.gene95020.t1 MTR_4g134660 76.471 238 52 3 21 257 3 237 9.49e-128 362
MS.gene95020.t1 MTR_4g070500 69.261 257 38 2 1 257 1 216 6.96e-101 293
MS.gene95020.t1 MTR_7g094510 29.703 202 125 7 26 218 8 201 1.72e-15 74.3
MS.gene95020.t1 MTR_7g080410 28.718 195 129 4 26 215 40 229 6.65e-15 73.2
MS.gene95020.t1 MTR_7g080410 28.718 195 129 4 26 215 40 229 1.13e-14 72.8
MS.gene95020.t1 MTR_3g085740 30.052 193 127 6 29 219 80 266 9.11e-14 70.1
MS.gene95020.t1 MTR_4g131740 25.641 195 131 4 27 213 43 231 3.16e-13 68.6
MS.gene95020.t1 MTR_8g044240 31.683 202 122 9 29 220 74 269 3.59e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95020.t1 AT1G10310 65.801 231 73 3 27 257 18 242 5.12e-109 315
MS.gene95020.t1 AT3G51680 29.612 206 135 5 27 226 35 236 4.60e-14 70.9
MS.gene95020.t1 AT5G65205 29.146 199 124 7 23 217 5 190 2.42e-12 65.9
MS.gene95020.t1 AT5G04900 30.500 200 126 7 29 220 82 276 9.23e-11 61.6

Find 82 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCATTTCATTCTTGTTCT+TGG 0.138262 4.4:-40097785 None:intergenic
GCCTTCGAAGCCCATGATTC+TGG 0.190516 4.4:-40098021 None:intergenic
GCCCATTTGGATGCACAATA+AGG 0.287508 4.4:-40097865 None:intergenic
TATTCATCAGCGTTGATGTT+AGG 0.357492 4.4:+40097560 MS.gene95020:CDS
GAGTCCTTCAATTGCCCATT+TGG 0.357810 4.4:-40097878 None:intergenic
CCTTCGAAGCCCATGATTCT+GGG 0.369639 4.4:-40098020 None:intergenic
AGTGAACATGTCTTCTGGTT+GGG 0.374134 4.4:+40097816 MS.gene95020:CDS
AGAGATGGCGCGCATTGTTA+TGG 0.379378 4.4:+40097600 MS.gene95020:CDS
GCACCGTTCACTATAATATC+TGG 0.390722 4.4:-40097640 None:intergenic
ATAATAGTGAACATGTCTTC+TGG 0.395450 4.4:+40097811 MS.gene95020:CDS
AAAGGGATCGAAATGGGAAT+TGG 0.400630 4.4:+40097361 MS.gene95020:CDS
CACCTTATTGTGCATCCAAA+TGG 0.401520 4.4:+40097863 MS.gene95020:CDS
AAGGGCTCTTGCAATTGAAT+TGG 0.401616 4.4:+40097444 MS.gene95020:CDS
ATCACAGGAACATCCGATAA+TGG 0.403722 4.4:-40097482 None:intergenic
AGTGAGAAAGGTAGAGGTAA+AGG 0.410847 4.4:+40097343 MS.gene95020:CDS
GTTGGTAGTGGCAGTGAGAA+AGG 0.412992 4.4:+40097331 MS.gene95020:CDS
TGCCAAGAACAAGAATGAAA+TGG 0.414644 4.4:+40097783 MS.gene95020:CDS
TCTTCTGGTTGGGGAAGATC+AGG 0.419311 4.4:+40097826 MS.gene95020:CDS
TTTATCAATCCCCAGAATCA+TGG 0.444345 4.4:+40098010 MS.gene95020:CDS
GAGGCACTTCATTCCTCTGA+TGG 0.453689 4.4:+40097759 MS.gene95020:CDS
AGAGGTAAAGGGATCGAAAT+GGG 0.456259 4.4:+40097355 MS.gene95020:CDS
AATCGCGGCCACACCATTAT+CGG 0.456442 4.4:+40097469 MS.gene95020:CDS
GCCACCACCGCCATTCCTTT+AGG 0.464900 4.4:-40097925 None:intergenic
CAATTGCAAGAGCCCTTCCT+AGG 0.469612 4.4:-40097438 None:intergenic
GACATGCTTGCTTCATGCTA+TGG 0.471703 4.4:+40097973 MS.gene95020:CDS
AATCGAACAGTGATGATAAC+TGG 0.474119 4.4:+40097403 MS.gene95020:CDS
ACCTTATTGTGCATCCAAAT+GGG 0.481113 4.4:+40097864 MS.gene95020:CDS
GCCTAAAGGAATGGCGGTGG+TGG 0.488412 4.4:+40097924 MS.gene95020:CDS
ATCGAAATGGGAATTGGAAT+AGG 0.499510 4.4:+40097367 MS.gene95020:CDS
TAGAGGTAAAGGGATCGAAA+TGG 0.499693 4.4:+40097354 MS.gene95020:CDS
AGTGGCAGTGAGAAAGGTAG+AGG 0.502593 4.4:+40097337 MS.gene95020:CDS
GCCAAGAACAAGAATGAAAT+GGG 0.504753 4.4:+40097784 MS.gene95020:CDS
GTGAGAAAGGTAGAGGTAAA+GGG 0.505960 4.4:+40097344 MS.gene95020:CDS
TTATCAATCCCCAGAATCAT+GGG 0.513090 4.4:+40098011 MS.gene95020:CDS
AAGTGTAAGATTGAGTATCT+TGG 0.514356 4.4:-40098049 None:intergenic
GCATCCAAATGGGCAATTGA+AGG 0.518913 4.4:+40097874 MS.gene95020:CDS
GCGGTGGTGGCACTCAATCC+AGG 0.519784 4.4:+40097937 MS.gene95020:CDS
GTGAACATGTCTTCTGGTTG+GGG 0.524566 4.4:+40097817 MS.gene95020:CDS
GATATTATAGTGAACGGTGC+TGG 0.525245 4.4:+40097643 MS.gene95020:CDS
CCCAGAATCATGGGCTTCGA+AGG 0.526565 4.4:+40098020 MS.gene95020:CDS
AATATATGATGATTGGTGTT+GGG 0.528355 4.4:-40097541 None:intergenic
GAATATATGATGATTGGTGT+TGG 0.537619 4.4:-40097542 None:intergenic
ATAATTGGATTGGAGATGGT+TGG 0.538248 4.4:+40097313 None:intergenic
CAATTACAACGTTGAAGAGA+TGG 0.540416 4.4:+40097585 MS.gene95020:CDS
ATGTCAGTGTTGATGACTCC+TGG 0.543360 4.4:-40097955 None:intergenic
GCTGATGAATATATGATGAT+TGG 0.544797 4.4:-40097548 None:intergenic
TGAAGAGTTTGAGTCGGTGA+TGG 0.545928 4.4:+40097705 MS.gene95020:CDS
GATGATAACTGGTGTTAGCA+AGG 0.549991 4.4:+40097414 MS.gene95020:CDS
TAGTGAACATGTCTTCTGGT+TGG 0.550146 4.4:+40097815 MS.gene95020:CDS
ACTGGTGTTAGCAAGGGCCT+AGG 0.550702 4.4:+40097421 MS.gene95020:CDS
TTGTTCTTGGCAACCATCAG+AGG 0.550752 4.4:-40097772 None:intergenic
GTGTTAGCAAGGGCCTAGGA+AGG 0.552217 4.4:+40097425 MS.gene95020:CDS
AATCTTACACTTGCAAACAA+TGG 0.554112 4.4:+40098060 MS.gene95020:CDS
ATTGACAGTACCTTTCAAGT+TGG 0.563428 4.4:-40097731 None:intergenic
TGTTGGGAAGTTGAGATTGA+AGG 0.563538 4.4:-40097525 None:intergenic
AAATGGGAATTGGAATAGGA+AGG 0.568891 4.4:+40097371 MS.gene95020:CDS
GAGCTTATTATCATGATCAC+AGG 0.571570 4.4:-40097497 None:intergenic
CACCGACTCAAACTCTTCAG+AGG 0.574771 4.4:-40097701 None:intergenic
GTTGCTGAAGAGTTGCCTAA+AGG 0.577379 4.4:+40097910 MS.gene95020:CDS
TGTAAGATTGAGTATCTTGG+CGG 0.587109 4.4:-40098046 None:intergenic
GTGATGGATACCAACTTGAA+AGG 0.588454 4.4:+40097721 MS.gene95020:CDS
ATTGGAATAGGAAGGAGTGA+TGG 0.590882 4.4:+40097379 MS.gene95020:CDS
TTAACAAGAACTGTAAGATG+TGG 0.595455 4.4:+40097671 MS.gene95020:CDS
GGATTGGAGATGGTTGGTAG+TGG 0.596007 4.4:+40097319 None:intergenic
ACCGACTCAAACTCTTCAGA+GGG 0.596079 4.4:-40097700 None:intergenic
CTTCGAAGCCCATGATTCTG+GGG 0.601345 4.4:-40098019 None:intergenic
TAACAAGAACTGTAAGATGT+GGG 0.606132 4.4:+40097672 MS.gene95020:CDS
ATGATAACTGGTGTTAGCAA+GGG 0.608228 4.4:+40097415 MS.gene95020:CDS
TCCCTCTGAAGAGTTTGAGT+CGG 0.610106 4.4:+40097699 MS.gene95020:CDS
TGAAGAGTTGCCTAAAGGAA+TGG 0.610138 4.4:+40097915 MS.gene95020:CDS
GCAATTGAATTGGCTAATCG+CGG 0.621991 4.4:+40097454 MS.gene95020:CDS
CCTCCAGATATTATAGTGAA+CGG 0.626961 4.4:+40097637 MS.gene95020:CDS
TGTTAGCAAGGGCCTAGGAA+GGG 0.628655 4.4:+40097426 MS.gene95020:CDS
GTTGCCTAAAGGAATGGCGG+TGG 0.632507 4.4:+40097921 MS.gene95020:CDS
AAGAACAAGAATGAAATGGG+AGG 0.635978 4.4:+40097787 MS.gene95020:CDS
AGAACAAGAATGAAATGGGA+GGG 0.640953 4.4:+40097788 MS.gene95020:CDS
TGGGGATTGATAAAGAGATG+AGG 0.653991 4.4:-40098001 None:intergenic
AGGAACATCCGATAATGGTG+TGG 0.674252 4.4:-40097477 None:intergenic
CAAGAACTGTAAGATGTGGG+AGG 0.685100 4.4:+40097675 MS.gene95020:CDS
CCGTTCACTATAATATCTGG+AGG 0.688415 4.4:-40097637 None:intergenic
AGAGTTGCCTAAAGGAATGG+CGG 0.705144 4.4:+40097918 MS.gene95020:CDS
AAGGTACTGTCAATGTGTTG+AGG 0.776497 4.4:+40097740 MS.gene95020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATGATGATTGGTGTT+GGG - chr4.4:40097544-40097563 None:intergenic 25.0%
AAGTGTAAGATTGAGTATCT+TGG - chr4.4:40098052-40098071 None:intergenic 30.0%
AATCTTACACTTGCAAACAA+TGG + chr4.4:40098060-40098079 MS.gene95020:CDS 30.0%
ATAATAGTGAACATGTCTTC+TGG + chr4.4:40097811-40097830 MS.gene95020:CDS 30.0%
ATTGTTATGGAGAAAAAAGG+AGG + chr4.4:40097613-40097632 MS.gene95020:CDS 30.0%
GAATATATGATGATTGGTGT+TGG - chr4.4:40097545-40097564 None:intergenic 30.0%
GCTGATGAATATATGATGAT+TGG - chr4.4:40097551-40097570 None:intergenic 30.0%
TAACAAGAACTGTAAGATGT+GGG + chr4.4:40097672-40097691 MS.gene95020:CDS 30.0%
TTAACAAGAACTGTAAGATG+TGG + chr4.4:40097671-40097690 MS.gene95020:CDS 30.0%
AAATGGGAATTGGAATAGGA+AGG + chr4.4:40097371-40097390 MS.gene95020:CDS 35.0%
AAGAACAAGAATGAAATGGG+AGG + chr4.4:40097787-40097806 MS.gene95020:CDS 35.0%
AATCGAACAGTGATGATAAC+TGG + chr4.4:40097403-40097422 MS.gene95020:CDS 35.0%
ACCTTATTGTGCATCCAAAT+GGG + chr4.4:40097864-40097883 MS.gene95020:CDS 35.0%
AGAACAAGAATGAAATGGGA+GGG + chr4.4:40097788-40097807 MS.gene95020:CDS 35.0%
ATCGAAATGGGAATTGGAAT+AGG + chr4.4:40097367-40097386 MS.gene95020:CDS 35.0%
CAATTACAACGTTGAAGAGA+TGG + chr4.4:40097585-40097604 MS.gene95020:CDS 35.0%
CCTCCAGATATTATAGTGAA+CGG + chr4.4:40097637-40097656 MS.gene95020:CDS 35.0%
CGCATTGTTATGGAGAAAAA+AGG + chr4.4:40097610-40097629 MS.gene95020:CDS 35.0%
GAGCTTATTATCATGATCAC+AGG - chr4.4:40097500-40097519 None:intergenic 35.0%
GCCAAGAACAAGAATGAAAT+GGG + chr4.4:40097784-40097803 MS.gene95020:CDS 35.0%
TCCCATTTCATTCTTGTTCT+TGG - chr4.4:40097788-40097807 None:intergenic 35.0%
TGCCAAGAACAAGAATGAAA+TGG + chr4.4:40097783-40097802 MS.gene95020:CDS 35.0%
TGTAAGATTGAGTATCTTGG+CGG - chr4.4:40098049-40098068 None:intergenic 35.0%
TTATCAATCCCCAGAATCAT+GGG + chr4.4:40098011-40098030 MS.gene95020:CDS 35.0%
TTTATCAATCCCCAGAATCA+TGG + chr4.4:40098010-40098029 MS.gene95020:CDS 35.0%
! ATGATAACTGGTGTTAGCAA+GGG + chr4.4:40097415-40097434 MS.gene95020:CDS 35.0%
! ATTGACAGTACCTTTCAAGT+TGG - chr4.4:40097734-40097753 None:intergenic 35.0%
!! TATTCATCAGCGTTGATGTT+AGG + chr4.4:40097560-40097579 MS.gene95020:CDS 35.0%
AAAGGGATCGAAATGGGAAT+TGG + chr4.4:40097361-40097380 MS.gene95020:CDS 40.0%
AAGGGCTCTTGCAATTGAAT+TGG + chr4.4:40097444-40097463 MS.gene95020:CDS 40.0%
AGAGGTAAAGGGATCGAAAT+GGG + chr4.4:40097355-40097374 MS.gene95020:CDS 40.0%
AGTGAGAAAGGTAGAGGTAA+AGG + chr4.4:40097343-40097362 MS.gene95020:CDS 40.0%
ATCACAGGAACATCCGATAA+TGG - chr4.4:40097485-40097504 None:intergenic 40.0%
ATTGGAATAGGAAGGAGTGA+TGG + chr4.4:40097379-40097398 MS.gene95020:CDS 40.0%
CACCTTATTGTGCATCCAAA+TGG + chr4.4:40097863-40097882 MS.gene95020:CDS 40.0%
CCGTTCACTATAATATCTGG+AGG - chr4.4:40097640-40097659 None:intergenic 40.0%
GATATTATAGTGAACGGTGC+TGG + chr4.4:40097643-40097662 MS.gene95020:CDS 40.0%
GCAATTGAATTGGCTAATCG+CGG + chr4.4:40097454-40097473 MS.gene95020:CDS 40.0%
GCACCGTTCACTATAATATC+TGG - chr4.4:40097643-40097662 None:intergenic 40.0%
GTGAGAAAGGTAGAGGTAAA+GGG + chr4.4:40097344-40097363 MS.gene95020:CDS 40.0%
TAGAGGTAAAGGGATCGAAA+TGG + chr4.4:40097354-40097373 MS.gene95020:CDS 40.0%
TAGTGAACATGTCTTCTGGT+TGG + chr4.4:40097815-40097834 MS.gene95020:CDS 40.0%
TGAAGAGTTGCCTAAAGGAA+TGG + chr4.4:40097915-40097934 MS.gene95020:CDS 40.0%
TGGGGATTGATAAAGAGATG+AGG - chr4.4:40098004-40098023 None:intergenic 40.0%
TGTTGGGAAGTTGAGATTGA+AGG - chr4.4:40097528-40097547 None:intergenic 40.0%
! AGTGAACATGTCTTCTGGTT+GGG + chr4.4:40097816-40097835 MS.gene95020:CDS 40.0%
! GATGATAACTGGTGTTAGCA+AGG + chr4.4:40097414-40097433 MS.gene95020:CDS 40.0%
!! AAGGTACTGTCAATGTGTTG+AGG + chr4.4:40097740-40097759 MS.gene95020:CDS 40.0%
!! GTGATGGATACCAACTTGAA+AGG + chr4.4:40097721-40097740 MS.gene95020:CDS 40.0%
AGAGTTGCCTAAAGGAATGG+CGG + chr4.4:40097918-40097937 MS.gene95020:CDS 45.0%
CAAGAACTGTAAGATGTGGG+AGG + chr4.4:40097675-40097694 MS.gene95020:CDS 45.0%
GACATGCTTGCTTCATGCTA+TGG + chr4.4:40097973-40097992 MS.gene95020:CDS 45.0%
GAGTCCTTCAATTGCCCATT+TGG - chr4.4:40097881-40097900 None:intergenic 45.0%
GCATCCAAATGGGCAATTGA+AGG + chr4.4:40097874-40097893 MS.gene95020:CDS 45.0%
GCCCATTTGGATGCACAATA+AGG - chr4.4:40097868-40097887 None:intergenic 45.0%
GTTGCTGAAGAGTTGCCTAA+AGG + chr4.4:40097910-40097929 MS.gene95020:CDS 45.0%
TGAAGAGTTTGAGTCGGTGA+TGG + chr4.4:40097705-40097724 MS.gene95020:CDS 45.0%
TTGTTCTTGGCAACCATCAG+AGG - chr4.4:40097775-40097794 None:intergenic 45.0%
! ACCGACTCAAACTCTTCAGA+GGG - chr4.4:40097703-40097722 None:intergenic 45.0%
! AGGAACATCCGATAATGGTG+TGG - chr4.4:40097480-40097499 None:intergenic 45.0%
! GTGAACATGTCTTCTGGTTG+GGG + chr4.4:40097817-40097836 MS.gene95020:CDS 45.0%
! TCCCTCTGAAGAGTTTGAGT+CGG + chr4.4:40097699-40097718 MS.gene95020:CDS 45.0%
!! ATGTCAGTGTTGATGACTCC+TGG - chr4.4:40097958-40097977 None:intergenic 45.0%
AATCGCGGCCACACCATTAT+CGG + chr4.4:40097469-40097488 MS.gene95020:CDS 50.0%
AGAGATGGCGCGCATTGTTA+TGG + chr4.4:40097600-40097619 MS.gene95020:CDS 50.0%
AGTGGCAGTGAGAAAGGTAG+AGG + chr4.4:40097337-40097356 MS.gene95020:CDS 50.0%
CAATTGCAAGAGCCCTTCCT+AGG - chr4.4:40097441-40097460 None:intergenic 50.0%
CCTTCGAAGCCCATGATTCT+GGG - chr4.4:40098023-40098042 None:intergenic 50.0%
CTTCGAAGCCCATGATTCTG+GGG - chr4.4:40098022-40098041 None:intergenic 50.0%
TGTTAGCAAGGGCCTAGGAA+GGG + chr4.4:40097426-40097445 MS.gene95020:CDS 50.0%
! GAGGCACTTCATTCCTCTGA+TGG + chr4.4:40097759-40097778 MS.gene95020:CDS 50.0%
! TCTTCTGGTTGGGGAAGATC+AGG + chr4.4:40097826-40097845 MS.gene95020:CDS 50.0%
!! CACCGACTCAAACTCTTCAG+AGG - chr4.4:40097704-40097723 None:intergenic 50.0%
!! GTTGGTAGTGGCAGTGAGAA+AGG + chr4.4:40097331-40097350 MS.gene95020:CDS 50.0%
CCCAGAATCATGGGCTTCGA+AGG + chr4.4:40098020-40098039 MS.gene95020:CDS 55.0%
GCCTTCGAAGCCCATGATTC+TGG - chr4.4:40098024-40098043 None:intergenic 55.0%
GTGTTAGCAAGGGCCTAGGA+AGG + chr4.4:40097425-40097444 MS.gene95020:CDS 55.0%
GTTGCCTAAAGGAATGGCGG+TGG + chr4.4:40097921-40097940 MS.gene95020:CDS 55.0%
! ACTGGTGTTAGCAAGGGCCT+AGG + chr4.4:40097421-40097440 MS.gene95020:CDS 55.0%
GCCACCACCGCCATTCCTTT+AGG - chr4.4:40097928-40097947 None:intergenic 60.0%
GCCTAAAGGAATGGCGGTGG+TGG + chr4.4:40097924-40097943 MS.gene95020:CDS 60.0%
! GCGGTGGTGGCACTCAATCC+AGG + chr4.4:40097937-40097956 MS.gene95020:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 40097328 40098101 40097328 ID=MS.gene95020
chr4.4 mRNA 40097328 40098101 40097328 ID=MS.gene95020.t1;Parent=MS.gene95020
chr4.4 exon 40097328 40098101 40097328 ID=MS.gene95020.t1.exon1;Parent=MS.gene95020.t1
chr4.4 CDS 40097328 40098101 40097328 ID=cds.MS.gene95020.t1;Parent=MS.gene95020.t1
Gene Sequence

>MS.gene95020

ATGGTTGGTAGTGGCAGTGAGAAAGGTAGAGGTAAAGGGATCGAAATGGGAATTGGAATAGGAAGGAGTGATGGAAATCGAACAGTGATGATAACTGGTGTTAGCAAGGGCCTAGGAAGGGCTCTTGCAATTGAATTGGCTAATCGCGGCCACACCATTATCGGATGTTCCTGTGATCATGATAATAAGCTCGACTTCCTTCAATCTCAACTTCCCAACACCAATCATCATATATTCATCAGCGTTGATGTTAGGTGCAATTACAACGTTGAAGAGATGGCGCGCATTGTTATGGAGAAAAAAGGAGGTCCTCCAGATATTATAGTGAACGGTGCTGGTGTCATTAACAAGAACTGTAAGATGTGGGAGGTTCCCTCTGAAGAGTTTGAGTCGGTGATGGATACCAACTTGAAAGGTACTGTCAATGTGTTGAGGCACTTCATTCCTCTGATGGTTGCCAAGAACAAGAATGAAATGGGAGGGATAATAGTGAACATGTCTTCTGGTTGGGGAAGATCAGGTGCAGCACTTGTAGCACCTTATTGTGCATCCAAATGGGCAATTGAAGGACTCACTAAGTCTGTTGCTGAAGAGTTGCCTAAAGGAATGGCGGTGGTGGCACTCAATCCAGGAGTCATCAACACTGACATGCTTGCTTCATGCTATGGTGCTTCCTCATCTCTTTATCAATCCCCAGAATCATGGGCTTCGAAGGCAGCCGCCAAGATACTCAATCTTACACTTGCAAACAATGGAACTTCTCTCTCTATTTGA

Protein sequence

>MS.gene95020.t1

MVGSGSEKGRGKGIEMGIGIGRSDGNRTVMITGVSKGLGRALAIELANRGHTIIGCSCDHDNKLDFLQSQLPNTNHHIFISVDVRCNYNVEEMARIVMEKKGGPPDIIVNGAGVINKNCKMWEVPSEEFESVMDTNLKGTVNVLRHFIPLMVAKNKNEMGGIIVNMSSGWGRSGAALVAPYCASKWAIEGLTKSVAEELPKGMAVVALNPGVINTDMLASCYGASSSLYQSPESWASKAAAKILNLTLANNGTSLSI