AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54598


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54598.t1 MTR_4g066050 96.053 228 9 0 1 228 30 257 7.28e-164 452
MS.gene54598.t1 MTR_4g134660 79.039 229 44 3 1 228 12 237 1.98e-128 362
MS.gene54598.t1 MTR_4g070490 79.736 227 44 2 3 228 32 257 9.45e-126 356
MS.gene54598.t1 MTR_4g070500 68.860 228 30 2 1 228 30 216 4.77e-97 282
MS.gene54598.t1 MTR_7g080410 30.526 190 122 4 2 186 45 229 1.24e-15 74.7
MS.gene54598.t1 MTR_7g080410 30.526 190 122 4 2 186 45 229 1.65e-15 74.3
MS.gene54598.t1 MTR_7g094510 30.303 198 121 7 1 189 12 201 2.03e-14 70.5
MS.gene54598.t1 MTR_3g085740 30.729 192 125 6 1 190 81 266 4.65e-14 70.5
MS.gene54598.t1 MTR_4g131740 26.042 192 128 4 1 184 46 231 4.97e-14 70.1
MS.gene54598.t1 MTR_7g071680 29.442 197 127 5 1 193 35 223 3.87e-13 67.8
MS.gene54598.t1 MTR_8g044240 32.338 201 120 9 1 191 75 269 2.30e-12 65.5
MS.gene54598.t1 MTR_7g083870 31.707 205 118 8 1 201 41 227 2.69e-12 65.1
MS.gene54598.t1 MTR_4g064883 28.866 194 124 5 1 189 20 204 6.39e-11 60.5
MS.gene54598.t1 MTR_7g110140 28.866 194 131 4 1 193 12 199 6.50e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54598.t1 AT1G10310 67.105 228 69 3 1 228 21 242 3.11e-111 319
MS.gene54598.t1 AT3G51680 30.049 203 132 5 1 197 38 236 9.74e-15 72.0
MS.gene54598.t1 AT5G10050 30.435 207 126 8 1 204 11 202 5.22e-14 69.7
MS.gene54598.t1 AT1G24470 29.646 226 136 9 1 214 56 270 1.30e-12 66.2
MS.gene54598.t1 AT5G65205 29.474 190 121 6 1 188 12 190 2.77e-12 65.1
MS.gene54598.t1 AT5G04900 31.156 199 124 7 1 191 83 276 1.42e-11 63.5
MS.gene54598.t1 AT1G67730 27.854 219 139 7 1 210 55 263 4.11e-11 62.0

Find 73 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCATTTCATTCTTGTTCT+TGG 0.138262 4.3:+37925929 None:intergenic
GCCTCCGAAGCCCATGATTC+TGG 0.154630 4.3:+37925693 None:intergenic
GCCCATTTGGATGCACAATA+AGG 0.287508 4.3:+37925849 None:intergenic
TATTCATCAGCGTTGATGTT+AGG 0.357492 4.3:-37926154 MS.gene54598:CDS
GAGTCCTTCAATTGCCCATT+TGG 0.357810 4.3:+37925836 None:intergenic
CCTCCGAAGCCCATGATTCT+GGG 0.369639 4.3:+37925694 None:intergenic
AGTGAACATGTCTTCTGGTT+GGG 0.374134 4.3:-37925898 MS.gene54598:CDS
AGAGATGGCGCGCATTGTTA+TGG 0.379378 4.3:-37926114 MS.gene54598:CDS
GCACCGTTCACTATAATATC+TGG 0.390722 4.3:+37926074 None:intergenic
ATAATAGTGAACATGTCTTC+TGG 0.395450 4.3:-37925903 MS.gene54598:CDS
CACCTTATTGTGCATCCAAA+TGG 0.401520 4.3:-37925851 MS.gene54598:CDS
AAGGGCTCTTGCAATTGAAT+TGG 0.401616 4.3:-37926270 MS.gene54598:CDS
TGCCAAGAACAAGAATGAAA+TGG 0.414644 4.3:-37925931 MS.gene54598:CDS
ATCACGGGAACATCCGATAA+TGG 0.418389 4.3:+37926232 None:intergenic
TCTTCTGGTTGGGGAAGATC+AGG 0.419311 4.3:-37925888 MS.gene54598:CDS
TTTATCAATCCCCAGAATCA+TGG 0.444345 4.3:-37925704 MS.gene54598:CDS
GAGGCACTTCATTCCTCTGA+TGG 0.453689 4.3:-37925955 MS.gene54598:CDS
AATCGCGGCCACACCATTAT+CGG 0.456442 4.3:-37926245 MS.gene54598:CDS
ATCCCCAGAATCATGGGCTT+CGG 0.464636 4.3:-37925697 MS.gene54598:CDS
GCCACCACCGCCATTCCTTT+AGG 0.464900 4.3:+37925789 None:intergenic
CAATTGCAAGAGCCCTTCCT+AGG 0.469612 4.3:+37926276 None:intergenic
GACATGCTTGCTTCATGCTA+TGG 0.471703 4.3:-37925741 MS.gene54598:CDS
AATCGAACAGTGATGATAAC+TGG 0.474119 4.3:-37926311 None:intergenic
CAATTACAGCGTTGAAGAGA+TGG 0.475899 4.3:-37926129 MS.gene54598:CDS
ACCTTATTGTGCATCCAAAT+GGG 0.481113 4.3:-37925850 MS.gene54598:CDS
GCCTAAAGGAATGGCGGTGG+TGG 0.488412 4.3:-37925790 MS.gene54598:CDS
GCCAAGAACAAGAATGAAAT+GGG 0.504753 4.3:-37925930 MS.gene54598:CDS
TTATCAATCCCCAGAATCAT+GGG 0.513090 4.3:-37925703 MS.gene54598:CDS
GCATCCAAATGGGCAATTGA+AGG 0.518913 4.3:-37925840 MS.gene54598:CDS
GCGGTGGTGGCACTCAATCC+AGG 0.519784 4.3:-37925777 MS.gene54598:CDS
GTGAACATGTCTTCTGGTTG+GGG 0.524566 4.3:-37925897 MS.gene54598:CDS
GATATTATAGTGAACGGTGC+TGG 0.525245 4.3:-37926071 MS.gene54598:CDS
AATATATGATGATTGGTGTT+GGG 0.528355 4.3:+37926173 None:intergenic
CGAGCTTATTATCATGATCA+CGG 0.531066 4.3:+37926216 None:intergenic
TGTTGGGAAGTTGAGATTGA+AGG 0.536503 4.3:+37926189 None:intergenic
GAATATATGATGATTGGTGT+TGG 0.537619 4.3:+37926172 None:intergenic
ATGTCAGTGTTGATGACTCC+TGG 0.543360 4.3:+37925759 None:intergenic
GCTGATGAATATATGATGAT+TGG 0.544797 4.3:+37926166 None:intergenic
TGAAGAGTTTGAGTCGGTGA+TGG 0.545928 4.3:-37926009 MS.gene54598:CDS
GATGATAACTGGTGTTAGCA+AGG 0.549991 4.3:-37926300 MS.gene54598:CDS
TAGTGAACATGTCTTCTGGT+TGG 0.550146 4.3:-37925899 MS.gene54598:CDS
ACTGGTGTTAGCAAGGGCCT+AGG 0.550702 4.3:-37926293 MS.gene54598:CDS
TTGTTCTTGGCAACCATCAG+AGG 0.550752 4.3:+37925942 None:intergenic
GTGTTAGCAAGGGCCTAGGA+AGG 0.552217 4.3:-37926289 MS.gene54598:CDS
GAGCTTATTATCATGATCAC+GGG 0.556947 4.3:+37926217 None:intergenic
AAGTGTAAGATTGAGTATCT+TGG 0.558491 4.3:+37925665 None:intergenic
AATCTTACACTTGCAAACAA+TGG 0.564921 4.3:-37925654 MS.gene54598:CDS
ATTGGCAGTACCTTTCAAGT+TGG 0.566483 4.3:+37925983 None:intergenic
CCCAGAATCATGGGCTTCGG+AGG 0.572981 4.3:-37925694 MS.gene54598:CDS
CACCGACTCAAACTCTTCAG+AGG 0.574771 4.3:+37926013 None:intergenic
GTTGCTGAAGAGTTGCCTAA+AGG 0.577379 4.3:-37925804 MS.gene54598:CDS
GTGATGGATACCAACTTGAA+AGG 0.588454 4.3:-37925993 MS.gene54598:CDS
CTCCGAAGCCCATGATTCTG+GGG 0.593057 4.3:+37925695 None:intergenic
ACCGACTCAAACTCTTCAGA+GGG 0.596079 4.3:+37926014 None:intergenic
ATGATAACTGGTGTTAGCAA+GGG 0.608228 4.3:-37926299 MS.gene54598:CDS
TAACAAGAACAGTAAGATGT+GGG 0.608572 4.3:-37926042 MS.gene54598:CDS
TCCCTCTGAAGAGTTTGAGT+CGG 0.610106 4.3:-37926015 MS.gene54598:CDS
TGAAGAGTTGCCTAAAGGAA+TGG 0.610138 4.3:-37925799 MS.gene54598:CDS
TTAACAAGAACAGTAAGATG+TGG 0.610296 4.3:-37926043 MS.gene54598:CDS
AATGAAGTGCCTCAACACAT+TGG 0.620714 4.3:+37925965 None:intergenic
GCAATTGAATTGGCTAATCG+CGG 0.621991 4.3:-37926260 MS.gene54598:CDS
CCTCCAGATATTATAGTGAA+CGG 0.626961 4.3:-37926077 MS.gene54598:CDS
TGTTAGCAAGGGCCTAGGAA+GGG 0.628655 4.3:-37926288 MS.gene54598:CDS
GTTGCCTAAAGGAATGGCGG+TGG 0.632507 4.3:-37925793 MS.gene54598:CDS
AAGAACAAGAATGAAATGGG+AGG 0.635978 4.3:-37925927 MS.gene54598:CDS
AGAACAAGAATGAAATGGGA+GGG 0.640953 4.3:-37925926 MS.gene54598:CDS
TGTAAGATTGAGTATCTTGG+TGG 0.642521 4.3:+37925668 None:intergenic
TGGGGATTGATAAAGAGATG+AGG 0.653991 4.3:+37925713 None:intergenic
GGGAACATCCGATAATGGTG+TGG 0.674398 4.3:+37926237 None:intergenic
CAAGAACAGTAAGATGTGGG+AGG 0.687539 4.3:-37926039 MS.gene54598:CDS
CCGTTCACTATAATATCTGG+AGG 0.688415 4.3:+37926077 None:intergenic
AGAGTTGCCTAAAGGAATGG+CGG 0.705144 4.3:-37925796 MS.gene54598:CDS
AAGGTACTGCCAATGTGTTG+AGG 0.753722 4.3:-37925974 MS.gene54598:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATGATGATTGGTGTT+GGG + chr4.3:37925764-37925783 None:intergenic 25.0%
AAGTGTAAGATTGAGTATCT+TGG + chr4.3:37926272-37926291 None:intergenic 30.0%
AATCTTACACTTGCAAACAA+TGG - chr4.3:37926280-37926299 MS.gene54598:CDS 30.0%
ATAATAGTGAACATGTCTTC+TGG - chr4.3:37926031-37926050 MS.gene54598:CDS 30.0%
ATTGTTATGGAGAAAAAAGG+AGG - chr4.3:37925833-37925852 MS.gene54598:CDS 30.0%
GAATATATGATGATTGGTGT+TGG + chr4.3:37925765-37925784 None:intergenic 30.0%
GCTGATGAATATATGATGAT+TGG + chr4.3:37925771-37925790 None:intergenic 30.0%
TAACAAGAACAGTAAGATGT+GGG - chr4.3:37925892-37925911 MS.gene54598:CDS 30.0%
TTAACAAGAACAGTAAGATG+TGG - chr4.3:37925891-37925910 MS.gene54598:CDS 30.0%
AAGAACAAGAATGAAATGGG+AGG - chr4.3:37926007-37926026 MS.gene54598:CDS 35.0%
ACCTTATTGTGCATCCAAAT+GGG - chr4.3:37926084-37926103 MS.gene54598:CDS 35.0%
AGAACAAGAATGAAATGGGA+GGG - chr4.3:37926008-37926027 MS.gene54598:CDS 35.0%
CCTCCAGATATTATAGTGAA+CGG - chr4.3:37925857-37925876 MS.gene54598:CDS 35.0%
CGAGCTTATTATCATGATCA+CGG + chr4.3:37925721-37925740 None:intergenic 35.0%
CGCATTGTTATGGAGAAAAA+AGG - chr4.3:37925830-37925849 MS.gene54598:CDS 35.0%
GAGCTTATTATCATGATCAC+GGG + chr4.3:37925720-37925739 None:intergenic 35.0%
GCCAAGAACAAGAATGAAAT+GGG - chr4.3:37926004-37926023 MS.gene54598:CDS 35.0%
TCCCATTTCATTCTTGTTCT+TGG + chr4.3:37926008-37926027 None:intergenic 35.0%
TGCCAAGAACAAGAATGAAA+TGG - chr4.3:37926003-37926022 MS.gene54598:CDS 35.0%
TGTAAGATTGAGTATCTTGG+TGG + chr4.3:37926269-37926288 None:intergenic 35.0%
TTATCAATCCCCAGAATCAT+GGG - chr4.3:37926231-37926250 MS.gene54598:CDS 35.0%
TTTATCAATCCCCAGAATCA+TGG - chr4.3:37926230-37926249 MS.gene54598:CDS 35.0%
! ATGATAACTGGTGTTAGCAA+GGG - chr4.3:37925635-37925654 MS.gene54598:CDS 35.0%
!! TATTCATCAGCGTTGATGTT+AGG - chr4.3:37925780-37925799 MS.gene54598:CDS 35.0%
AAGGGCTCTTGCAATTGAAT+TGG - chr4.3:37925664-37925683 MS.gene54598:CDS 40.0%
CACCTTATTGTGCATCCAAA+TGG - chr4.3:37926083-37926102 MS.gene54598:CDS 40.0%
CCGTTCACTATAATATCTGG+AGG + chr4.3:37925860-37925879 None:intergenic 40.0%
GATATTATAGTGAACGGTGC+TGG - chr4.3:37925863-37925882 MS.gene54598:CDS 40.0%
GCAATTGAATTGGCTAATCG+CGG - chr4.3:37925674-37925693 MS.gene54598:CDS 40.0%
GCACCGTTCACTATAATATC+TGG + chr4.3:37925863-37925882 None:intergenic 40.0%
TAGTGAACATGTCTTCTGGT+TGG - chr4.3:37926035-37926054 MS.gene54598:CDS 40.0%
TGAAGAGTTGCCTAAAGGAA+TGG - chr4.3:37926135-37926154 MS.gene54598:CDS 40.0%
TGGGGATTGATAAAGAGATG+AGG + chr4.3:37926224-37926243 None:intergenic 40.0%
TGTTGGGAAGTTGAGATTGA+AGG + chr4.3:37925748-37925767 None:intergenic 40.0%
! AATGAAGTGCCTCAACACAT+TGG + chr4.3:37925972-37925991 None:intergenic 40.0%
! AGTGAACATGTCTTCTGGTT+GGG - chr4.3:37926036-37926055 MS.gene54598:CDS 40.0%
! ATTGGCAGTACCTTTCAAGT+TGG + chr4.3:37925954-37925973 None:intergenic 40.0%
! CAATTACAGCGTTGAAGAGA+TGG - chr4.3:37925805-37925824 MS.gene54598:CDS 40.0%
!! GTGATGGATACCAACTTGAA+AGG - chr4.3:37925941-37925960 MS.gene54598:CDS 40.0%
AGAGTTGCCTAAAGGAATGG+CGG - chr4.3:37926138-37926157 MS.gene54598:CDS 45.0%
ATCACGGGAACATCCGATAA+TGG + chr4.3:37925705-37925724 None:intergenic 45.0%
CAAGAACAGTAAGATGTGGG+AGG - chr4.3:37925895-37925914 MS.gene54598:CDS 45.0%
GACATGCTTGCTTCATGCTA+TGG - chr4.3:37926193-37926212 MS.gene54598:CDS 45.0%
GAGTCCTTCAATTGCCCATT+TGG + chr4.3:37926101-37926120 None:intergenic 45.0%
GCATCCAAATGGGCAATTGA+AGG - chr4.3:37926094-37926113 MS.gene54598:CDS 45.0%
GCCCATTTGGATGCACAATA+AGG + chr4.3:37926088-37926107 None:intergenic 45.0%
GTTGCTGAAGAGTTGCCTAA+AGG - chr4.3:37926130-37926149 MS.gene54598:CDS 45.0%
TGAAGAGTTTGAGTCGGTGA+TGG - chr4.3:37925925-37925944 MS.gene54598:CDS 45.0%
TTGTTCTTGGCAACCATCAG+AGG + chr4.3:37925995-37926014 None:intergenic 45.0%
! ACCGACTCAAACTCTTCAGA+GGG + chr4.3:37925923-37925942 None:intergenic 45.0%
! GTGAACATGTCTTCTGGTTG+GGG - chr4.3:37926037-37926056 MS.gene54598:CDS 45.0%
! TCCCTCTGAAGAGTTTGAGT+CGG - chr4.3:37925919-37925938 MS.gene54598:CDS 45.0%
!! AAGGTACTGCCAATGTGTTG+AGG - chr4.3:37925960-37925979 MS.gene54598:CDS 45.0%
!! ATGTCAGTGTTGATGACTCC+TGG + chr4.3:37926178-37926197 None:intergenic 45.0%
AATCGCGGCCACACCATTAT+CGG - chr4.3:37925689-37925708 MS.gene54598:CDS 50.0%
AGAGATGGCGCGCATTGTTA+TGG - chr4.3:37925820-37925839 MS.gene54598:CDS 50.0%
ATCCCCAGAATCATGGGCTT+CGG - chr4.3:37926237-37926256 MS.gene54598:CDS 50.0%
CAATTGCAAGAGCCCTTCCT+AGG + chr4.3:37925661-37925680 None:intergenic 50.0%
TGTTAGCAAGGGCCTAGGAA+GGG - chr4.3:37925646-37925665 MS.gene54598:CDS 50.0%
! GAGGCACTTCATTCCTCTGA+TGG - chr4.3:37925979-37925998 MS.gene54598:CDS 50.0%
! GGGAACATCCGATAATGGTG+TGG + chr4.3:37925700-37925719 None:intergenic 50.0%
! TCTTCTGGTTGGGGAAGATC+AGG - chr4.3:37926046-37926065 MS.gene54598:CDS 50.0%
!! CACCGACTCAAACTCTTCAG+AGG + chr4.3:37925924-37925943 None:intergenic 50.0%
CCTCCGAAGCCCATGATTCT+GGG + chr4.3:37926243-37926262 None:intergenic 55.0%
CTCCGAAGCCCATGATTCTG+GGG + chr4.3:37926242-37926261 None:intergenic 55.0%
GTGTTAGCAAGGGCCTAGGA+AGG - chr4.3:37925645-37925664 MS.gene54598:CDS 55.0%
GTTGCCTAAAGGAATGGCGG+TGG - chr4.3:37926141-37926160 MS.gene54598:CDS 55.0%
! ACTGGTGTTAGCAAGGGCCT+AGG - chr4.3:37925641-37925660 MS.gene54598:CDS 55.0%
CCCAGAATCATGGGCTTCGG+AGG - chr4.3:37926240-37926259 MS.gene54598:CDS 60.0%
GCCACCACCGCCATTCCTTT+AGG + chr4.3:37926148-37926167 None:intergenic 60.0%
GCCTAAAGGAATGGCGGTGG+TGG - chr4.3:37926144-37926163 MS.gene54598:CDS 60.0%
GCCTCCGAAGCCCATGATTC+TGG + chr4.3:37926244-37926263 None:intergenic 60.0%
! GCGGTGGTGGCACTCAATCC+AGG - chr4.3:37926157-37926176 MS.gene54598:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37925635 37926321 37925635 ID=MS.gene54598
chr4.3 mRNA 37925635 37926321 37925635 ID=MS.gene54598.t1;Parent=MS.gene54598
chr4.3 exon 37925635 37926321 37925635 ID=MS.gene54598.t1.exon1;Parent=MS.gene54598.t1
chr4.3 CDS 37925635 37926321 37925635 ID=cds.MS.gene54598.t1;Parent=MS.gene54598.t1
Gene Sequence

>MS.gene54598

ATGATAACTGGTGTTAGCAAGGGCCTAGGAAGGGCTCTTGCAATTGAATTGGCTAATCGCGGCCACACCATTATCGGATGTTCCCGTGATCATGATAATAAGCTCGAATTCCTTCAATCTCAACTTCCCAACACCAATCATCATATATTCATCAGCGTTGATGTTAGGTGCAATTACAGCGTTGAAGAGATGGCGCGCATTGTTATGGAGAAAAAAGGAGGTCCTCCAGATATTATAGTGAACGGTGCTGGTGTCATTAACAAGAACAGTAAGATGTGGGAGGTTCCCTCTGAAGAGTTTGAGTCGGTGATGGATACCAACTTGAAAGGTACTGCCAATGTGTTGAGGCACTTCATTCCTCTGATGGTTGCCAAGAACAAGAATGAAATGGGAGGGATAATAGTGAACATGTCTTCTGGTTGGGGAAGATCAGGTGCAGCACTTGTAGCACCTTATTGTGCATCCAAATGGGCAATTGAAGGACTCACTAAGTCTGTTGCTGAAGAGTTGCCTAAAGGAATGGCGGTGGTGGCACTCAATCCAGGAGTCATCAACACTGACATGCTTGCTTCATGCTATGGTGCTTCCTCATCTCTTTATCAATCCCCAGAATCATGGGCTTCGGAGGCAGCCACCAAGATACTCAATCTTACACTTGCAAACAATGGAGCTTCTCTCTCTATTTGA

Protein sequence

>MS.gene54598.t1

MITGVSKGLGRALAIELANRGHTIIGCSRDHDNKLEFLQSQLPNTNHHIFISVDVRCNYSVEEMARIVMEKKGGPPDIIVNGAGVINKNSKMWEVPSEEFESVMDTNLKGTANVLRHFIPLMVAKNKNEMGGIIVNMSSGWGRSGAALVAPYCASKWAIEGLTKSVAEELPKGMAVVALNPGVINTDMLASCYGASSSLYQSPESWASEAATKILNLTLANNGASLSI