AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79400.t1 MTR_3g026020 94.845 194 5 1 1 194 1 189 2.04e-126 354
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79400.t1 AT5G08050 63.190 163 45 2 31 192 8 156 3.67e-51 162

Find 40 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGTCGAGGTTGGATTAC+AGG 0.269782 3.1:+16885791 MS.gene79400:CDS
GAGACAGATGGAACTGGTTT+GGG 0.332824 3.1:-16884270 None:intergenic
AGAGACAGATGGAACTGGTT+TGG 0.346099 3.1:-16884271 None:intergenic
TGTGTTGTTGGTGGTTCTGC+TGG 0.362863 3.1:+16885831 MS.gene79400:CDS
TAAAACTGTAGCTTCTAAAA+AGG 0.383526 3.1:+16884293 MS.gene79400:CDS
GTTGCTTTGGTCTGTGTTGT+TGG 0.388658 3.1:+16885819 MS.gene79400:CDS
ATTACAGGCTGTGGTTGCTT+TGG 0.392075 3.1:+16885806 MS.gene79400:CDS
TAGCGATAATCCTGCTTTCT+CGG 0.404741 3.1:-16885613 None:intergenic
CATTCTTGTGAGGAGTTTAA+CGG 0.405852 3.1:-16885538 None:intergenic
TAAACCTGCTTTCTCTGCCT+TGG 0.422974 3.1:-16885580 None:intergenic
AACCTCGACAACATTATCTT+CGG 0.427394 3.1:-16885781 None:intergenic
CGTTAAACTCCTCACAAGAA+TGG 0.467112 3.1:+16885539 MS.gene79400:CDS
GCCATGGATTTGACCAGAAA+AGG 0.471154 3.1:-16884243 None:intergenic
AAGAGTGATCCTGGAGTTGA+TGG 0.471176 3.1:-16885705 None:intergenic
GATCAAGCTGTTACTACAGA+TGG 0.487910 3.1:-16884178 None:intergenic
CTATCAACAATAGAGAAACT+TGG 0.493062 3.1:+16885633 MS.gene79400:CDS
CTGAGTGTGAAGAGTGATCC+TGG 0.494258 3.1:-16885714 None:intergenic
ACCTCGACAACATTATCTTC+GGG 0.509562 3.1:-16885780 None:intergenic
GAAGATAATGTTGTCGAGGT+TGG 0.509847 3.1:+16885783 MS.gene79400:CDS
TTATCAGCCGCCGAGAAAGC+AGG 0.520670 3.1:+16885603 MS.gene79400:CDS
AACTGGTTTGGGAGTAGCCA+TGG 0.526284 3.1:-16884259 None:intergenic
GCTTTGGTCTGTGTTGTTGG+TGG 0.531295 3.1:+16885822 MS.gene79400:CDS
CGAGGTTGGATTACAGGCTG+TGG 0.533900 3.1:+16885797 MS.gene79400:CDS
AATTCAACTGTATTCCCACT+TGG 0.537612 3.1:+16885480 MS.gene79400:CDS
CTCTCAAAAGCAGAAGAACT+CGG 0.539080 3.1:+16885660 MS.gene79400:CDS
TTAACCAAGGCAGAGAAAGC+AGG 0.541705 3.1:+16885576 MS.gene79400:CDS
GCTACTGATCCATCAACTCC+AGG 0.551246 3.1:+16885696 MS.gene79400:CDS
ATTCAACTGTATTCCCACTT+GGG 0.553323 3.1:+16885481 MS.gene79400:CDS
ACTTATGATGATGACAATGA+AGG 0.557494 3.1:+16884146 None:intergenic
TCAGTTGTTCCATTCTTGTG+AGG 0.560445 3.1:-16885548 None:intergenic
TCCGTGGTTGTTCCCCAAGT+GGG 0.573088 3.1:-16885494 None:intergenic
TTCAACTGTATTCCCACTTG+GGG 0.603934 3.1:+16885482 MS.gene79400:CDS
TCCCGAAGATAATGTTGTCG+AGG 0.604575 3.1:+16885779 MS.gene79400:CDS
CTCCGTGGTTGTTCCCCAAG+TGG 0.614234 3.1:-16885495 None:intergenic
GGAACAAGATAAACAAACAA+AGG 0.614999 3.1:-16885759 None:intergenic
TCCCACTTGGGGAACAACCA+CGG 0.646839 3.1:+16885493 MS.gene79400:CDS
GTTACTACAGATGGATGCTG+AGG 0.660105 3.1:-16884169 None:intergenic
GTCGATGTTACAGCACTCCG+TGG 0.693005 3.1:-16885510 None:intergenic
CGATAATCCTGCTTTCTCGG+CGG 0.695629 3.1:-16885610 None:intergenic
ACAACTGAGACTGTTAACCA+AGG 0.730927 3.1:+16885563 MS.gene79400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACATAAAAATTTCTATGT+AGG - chr3.1:16884867-16884886 None:intergenic 15.0%
!! TAATATTGCATAATGATAAA+AGG - chr3.1:16884392-16884411 None:intergenic 15.0%
!!! ATGTTATCAAACTTTTAAGT+AGG + chr3.1:16884881-16884900 MS.gene79400:intron 20.0%
!!! ATTATGTAAACCATGTTTTT+TGG + chr3.1:16885035-16885054 MS.gene79400:intron 20.0%
!!! TAGAAATGCTTTGTTTAAAT+AGG - chr3.1:16885006-16885025 None:intergenic 20.0%
!!! TATATTTTCAAAAAATCACC+TGG + chr3.1:16884809-16884828 MS.gene79400:intron 20.0%
!!! TTATGTAAACCATGTTTTTT+GGG + chr3.1:16885036-16885055 MS.gene79400:intron 20.0%
!!! TTATTTTATTTTCTGCAAGT+TGG - chr3.1:16885880-16885899 None:intergenic 20.0%
! AACCTTTCTCATAATTAAAG+TGG - chr3.1:16884454-16884473 None:intergenic 25.0%
! ATTGAAAAGGTTAAATAACC+AGG - chr3.1:16884830-16884849 None:intergenic 25.0%
! CAAACTCCTATAACAAATTA+CGG - chr3.1:16884596-16884615 None:intergenic 25.0%
! TACCACTTTAATTATGAGAA+AGG + chr3.1:16884449-16884468 MS.gene79400:intron 25.0%
! TTATGCAATATTATACTGTG+TGG + chr3.1:16884399-16884418 MS.gene79400:intron 25.0%
!! AAACAAAGCATTTCTAAACA+TGG + chr3.1:16885010-16885029 MS.gene79400:intron 25.0%
!! AGGAATCTTTAGGAAAAAAA+GGG + chr3.1:16884783-16884802 MS.gene79400:intron 25.0%
!! TAAAACTGTAGCTTCTAAAA+AGG + chr3.1:16884293-16884312 MS.gene79400:CDS 25.0%
!! TAGAATTTTCCCAAAAAACA+TGG - chr3.1:16885048-16885067 None:intergenic 25.0%
!!! ACTAGTTCTAAAAAACATCT+GGG + chr3.1:16885204-16885223 MS.gene79400:intron 25.0%
!!! AGCTTTGTTTTACTTCTTTT+AGG + chr3.1:16885735-16885754 MS.gene79400:CDS 25.0%
!!! CATAAACCGTAATTTGTTAT+AGG + chr3.1:16884587-16884606 MS.gene79400:intron 25.0%
!!! TACTAGTTCTAAAAAACATC+TGG + chr3.1:16885203-16885222 MS.gene79400:intron 25.0%
!!! TGTTTTTTGGGAAAATTCTA+TGG + chr3.1:16885048-16885067 MS.gene79400:intron 25.0%
ACTCATATGGTAAAAGACTA+AGG + chr3.1:16884672-16884691 MS.gene79400:intron 30.0%
ATTACGGTTTATGTATACCT+AGG - chr3.1:16884580-16884599 None:intergenic 30.0%
CTATCAACAATAGAGAAACT+TGG + chr3.1:16885633-16885652 MS.gene79400:CDS 30.0%
GGAACAAGATAAACAAACAA+AGG - chr3.1:16885762-16885781 None:intergenic 30.0%
TGTGTGTTCTATCAAAACAA+AGG - chr3.1:16885268-16885287 None:intergenic 30.0%
! ACAGACTTGTGTAAAACTTT+TGG + chr3.1:16884637-16884656 MS.gene79400:intron 30.0%
! ACTTTTCTCACCAAAAAAAC+AGG - chr3.1:16885420-16885439 None:intergenic 30.0%
! CAGGAATCTTTAGGAAAAAA+AGG + chr3.1:16884782-16884801 MS.gene79400:intron 30.0%
! TGCTCTTTGATGATATGATA+TGG + chr3.1:16884554-16884573 MS.gene79400:intron 30.0%
!! ATGCTTTGTTTAAATAGGTC+TGG - chr3.1:16885001-16885020 None:intergenic 30.0%
!! TCATCAAAGAGCATTAAAAC+TGG - chr3.1:16884547-16884566 None:intergenic 30.0%
!! TCTCTACGATAATTTTCAAG+TGG + chr3.1:16885378-16885397 MS.gene79400:intron 30.0%
!! TTTTTGGGAAAATTCTATGG+TGG + chr3.1:16885051-16885070 MS.gene79400:intron 30.0%
AACCTCGACAACATTATCTT+CGG - chr3.1:16885784-16885803 None:intergenic 35.0%
AATTCAACTGTATTCCCACT+TGG + chr3.1:16885480-16885499 MS.gene79400:CDS 35.0%
ACCATATGAGTTGTACTTAC+CGG - chr3.1:16884663-16884682 None:intergenic 35.0%
ATTCAACTGTATTCCCACTT+GGG + chr3.1:16885481-16885500 MS.gene79400:CDS 35.0%
CATTCTTGTGAGGAGTTTAA+CGG - chr3.1:16885541-16885560 None:intergenic 35.0%
CTATGACTCATAGTACTTGT+TGG + chr3.1:16885125-16885144 MS.gene79400:intron 35.0%
CTTTGTCTTAATGAGTGAGA+CGG + chr3.1:16885168-16885187 MS.gene79400:intron 35.0%
GGTCTGACAAGTACTAAAAA+TGG + chr3.1:16884927-16884946 MS.gene79400:intron 35.0%
GTACTATGAGTCATAGTTTC+AGG - chr3.1:16885121-16885140 None:intergenic 35.0%
TCTCACTCATTAAGACAAAG+AGG - chr3.1:16885168-16885187 None:intergenic 35.0%
TTTAAGTAGGCTAATACGTC+AGG + chr3.1:16884894-16884913 MS.gene79400:intron 35.0%
! ACAGTCATTGTTGCTGTTAT+AGG - chr3.1:16885311-16885330 None:intergenic 35.0%
! ACTTGTGTAAAACTTTTGGC+CGG + chr3.1:16884641-16884660 MS.gene79400:intron 35.0%
! GTTTTGTAGCAGGAATCTTT+AGG + chr3.1:16884773-16884792 MS.gene79400:intron 35.0%
! TAGTACTTGTCAGACCTTTT+CGG - chr3.1:16884923-16884942 None:intergenic 35.0%
! TGTTGCTGTTATAGGTGATA+CGG - chr3.1:16885303-16885322 None:intergenic 35.0%
! TTGATGATATGATATGGCCT+AGG + chr3.1:16884560-16884579 MS.gene79400:intron 35.0%
!! AAAAAAACAGGCTGGTTCTA+CGG - chr3.1:16885408-16885427 None:intergenic 35.0%
!! ACACAAAGTGTTGAGAAGTA+GGG - chr3.1:16884739-16884758 None:intergenic 35.0%
!! CATCGGTTGTTACTTGTTAT+TGG + chr3.1:16885353-16885372 MS.gene79400:intron 35.0%
ACAACTGAGACTGTTAACCA+AGG + chr3.1:16885563-16885582 MS.gene79400:CDS 40.0%
ACCTCGACAACATTATCTTC+GGG - chr3.1:16885783-16885802 None:intergenic 40.0%
CGTTAAACTCCTCACAAGAA+TGG + chr3.1:16885539-16885558 MS.gene79400:CDS 40.0%
CTCTCAAAAGCAGAAGAACT+CGG + chr3.1:16885660-16885679 MS.gene79400:CDS 40.0%
GAAGATAATGTTGTCGAGGT+TGG + chr3.1:16885783-16885802 MS.gene79400:CDS 40.0%
GATCAAGCTGTTACTACAGA+TGG - chr3.1:16884181-16884200 None:intergenic 40.0%
TAGCGATAATCCTGCTTTCT+CGG - chr3.1:16885616-16885635 None:intergenic 40.0%
TCAGTTGTTCCATTCTTGTG+AGG - chr3.1:16885551-16885570 None:intergenic 40.0%
TTCAACTGTATTCCCACTTG+GGG + chr3.1:16885482-16885501 MS.gene79400:CDS 40.0%
TTCTCACCAAAAAAACAGGC+TGG - chr3.1:16885416-16885435 None:intergenic 40.0%
! AGTGTTGAGAAGTAGGGAAT+CGG - chr3.1:16884733-16884752 None:intergenic 40.0%
! TCACACTTTCTCACCTTTTC+TGG + chr3.1:16884230-16884249 MS.gene79400:CDS 40.0%
!! ACCTTTTCTGGTCAAATCCA+TGG + chr3.1:16884242-16884261 MS.gene79400:CDS 40.0%
!! AGAGTTTTGTGTTGAAGGAG+AGG - chr3.1:16884211-16884230 None:intergenic 40.0%
!! CACACAAAGTGTTGAGAAGT+AGG - chr3.1:16884740-16884759 None:intergenic 40.0%
!! CATTTGATTTGCTCAGAAGC+AGG + chr3.1:16884698-16884717 MS.gene79400:intron 40.0%
!! GTGTGAGAGTTTTGTGTTGA+AGG - chr3.1:16884216-16884235 None:intergenic 40.0%
!!! GCTACAGTTTTAGAGACAGA+TGG - chr3.1:16884285-16884304 None:intergenic 40.0%
!!! GTAGAACCAGCCTGTTTTTT+TGG + chr3.1:16885407-16885426 MS.gene79400:intron 40.0%
!!! GTTTTAGAGACAGATGGAAC+TGG - chr3.1:16884279-16884298 None:intergenic 40.0%
AAGAGTGATCCTGGAGTTGA+TGG - chr3.1:16885708-16885727 None:intergenic 45.0%
AGAGACAGATGGAACTGGTT+TGG - chr3.1:16884274-16884293 None:intergenic 45.0%
AGGTGATACGGTGACTTGAT+TGG - chr3.1:16885291-16885310 None:intergenic 45.0%
ATTACAGGCTGTGGTTGCTT+TGG + chr3.1:16885806-16885825 MS.gene79400:CDS 45.0%
CTATGGTGGTCCACTTAGAT+AGG + chr3.1:16885065-16885084 MS.gene79400:intron 45.0%
GCCATGGATTTGACCAGAAA+AGG - chr3.1:16884246-16884265 None:intergenic 45.0%
GCCGGTAAGTACAACTCATA+TGG + chr3.1:16884659-16884678 MS.gene79400:intron 45.0%
GTGTTGAGAAGTAGGGAATC+GGG - chr3.1:16884732-16884751 None:intergenic 45.0%
GTTACTACAGATGGATGCTG+AGG - chr3.1:16884172-16884191 None:intergenic 45.0%
TAAACCTGCTTTCTCTGCCT+TGG - chr3.1:16885583-16885602 None:intergenic 45.0%
TCCACTTAGATAGGTGTCCA+CGG + chr3.1:16885074-16885093 MS.gene79400:intron 45.0%
TCCCGAAGATAATGTTGTCG+AGG + chr3.1:16885779-16885798 MS.gene79400:CDS 45.0%
TCTACTTCCAAGAGCGACAT+CGG + chr3.1:16885336-16885355 MS.gene79400:intron 45.0%
TGTTGTCGAGGTTGGATTAC+AGG + chr3.1:16885791-16885810 MS.gene79400:CDS 45.0%
TTAACCAAGGCAGAGAAAGC+AGG + chr3.1:16885576-16885595 MS.gene79400:CDS 45.0%
! GAGACAGATGGAACTGGTTT+GGG - chr3.1:16884273-16884292 None:intergenic 45.0%
! GTTGCTTTGGTCTGTGTTGT+TGG + chr3.1:16885819-16885838 MS.gene79400:CDS 45.0%
! TACTTGTCAGACCTTTTCGG+AGG - chr3.1:16884920-16884939 None:intergenic 45.0%
!! CGAGTTCTTCTGCTTTTGAG+AGG - chr3.1:16885662-16885681 None:intergenic 45.0%
!! GTGAGTGTGAGTTTTGTAGC+AGG + chr3.1:16884763-16884782 MS.gene79400:intron 45.0%
!! TAATAATTAAATTATTGAAA+AGG - chr3.1:16884843-16884862 None:intergenic 5.0%
AATACGTCAGGCCTCCGAAA+AGG + chr3.1:16884906-16884925 MS.gene79400:intron 50.0%
ACCGTGGACACCTATCTAAG+TGG - chr3.1:16885078-16885097 None:intergenic 50.0%
ACTTAGATAGGTGTCCACGG+TGG + chr3.1:16885077-16885096 MS.gene79400:intron 50.0%
CGATAATCCTGCTTTCTCGG+CGG - chr3.1:16885613-16885632 None:intergenic 50.0%
CTGAGTGTGAAGAGTGATCC+TGG - chr3.1:16885717-16885736 None:intergenic 50.0%
GCTACTGATCCATCAACTCC+AGG + chr3.1:16885696-16885715 MS.gene79400:CDS 50.0%
GTAACAACCGATGTCGCTCT+TGG - chr3.1:16885346-16885365 None:intergenic 50.0%
! GCTTTGGTCTGTGTTGTTGG+TGG + chr3.1:16885822-16885841 MS.gene79400:CDS 50.0%
!! AACTGGTTTGGGAGTAGCCA+TGG - chr3.1:16884262-16884281 None:intergenic 50.0%
!! TGTGTTGTTGGTGGTTCTGC+TGG + chr3.1:16885831-16885850 MS.gene79400:CDS 50.0%
!! TTTCAAGCGTTGATCCACCG+TGG - chr3.1:16885094-16885113 None:intergenic 50.0%
CGAGGTTGGATTACAGGCTG+TGG + chr3.1:16885797-16885816 MS.gene79400:CDS 55.0%
GAGTGAGACGGTGACTCGAT+TGG + chr3.1:16885180-16885199 MS.gene79400:intron 55.0%
GTCGATGTTACAGCACTCCG+TGG - chr3.1:16885513-16885532 None:intergenic 55.0%
TCCCACTTGGGGAACAACCA+CGG + chr3.1:16885493-16885512 MS.gene79400:CDS 55.0%
TCCGTGGTTGTTCCCCAAGT+GGG - chr3.1:16885497-16885516 None:intergenic 55.0%
TTATCAGCCGCCGAGAAAGC+AGG + chr3.1:16885603-16885622 MS.gene79400:CDS 55.0%
!!! GGTGGTTCTGCTGGTTTTGC+TGG + chr3.1:16885840-16885859 MS.gene79400:CDS 55.0%
CTCCGTGGTTGTTCCCCAAG+TGG - chr3.1:16885498-16885517 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 16884150 16885899 16884150 ID=MS.gene79400
chr3.1 mRNA 16884150 16885899 16884150 ID=MS.gene79400.t1;Parent=MS.gene79400
chr3.1 exon 16884150 16884314 16884150 ID=MS.gene79400.t1.exon1;Parent=MS.gene79400.t1
chr3.1 CDS 16884150 16884314 16884150 ID=cds.MS.gene79400.t1;Parent=MS.gene79400.t1
chr3.1 exon 16885480 16885899 16885480 ID=MS.gene79400.t1.exon2;Parent=MS.gene79400.t1
chr3.1 CDS 16885480 16885899 16885480 ID=cds.MS.gene79400.t1;Parent=MS.gene79400.t1
Gene Sequence

>MS.gene79400

ATGATGATGACAATGAAGGCCTCAGCATCCATCTGTAGTAACAGCTTGATCTTCTACTCCTCTCCTTCAACACAAAACTCTCACACTTTCTCACCTTTTCTGGTCAAATCCATGGCTACTCCCAAACCAGTTCCATCTGTCTCTAAAACTGTAGCTTCTAAAAAGGTCAGTGCAAAACATTAACTATATAATATTACTTGTTAGTATGATTTTTGATGATTTACATAATTGCTTTAATTCCTTTTATCATTATGCAATATTATACTGTGTGGAACTAGAACATGTCTATAATATATATTTACCACTTTAATTATGAGAAAGGTTGAAAAACTAGCTCTTATAATATTGTAAGACAAATCTGTGAAATTTAGCAATATGTTAACAGCACTGAACACCAGTTTTAATGCTCTTTGATGATATGATATGGCCTAGGTATACATAAACCGTAATTTGTTATAGGAGTTTGTAGTTTATTGATCATTAAGAAACAGACTTGTGTAAAACTTTTGGCCGGTAAGTACAACTCATATGGTAAAAGACTAAGGAGACATTTGATTTGCTCAGAAGCAGGTTTGAACTCCCGATTCCCTACTTCTCAACACTTTGTGTGTGTGTGAGTGTGAGTTTTGTAGCAGGAATCTTTAGGAAAAAAAGGGAACTATATTTTCAAAAAATCACCTGGTTATTTAACCTTTTCAATAATTTAATTATTAGCCTACATAGAAATTTTTATGTTATCAAACTTTTAAGTAGGCTAATACGTCAGGCCTCCGAAAAGGTCTGACAAGTACTAAAAATGGCGTATGATAGTGAACTTATCATGTTTAGAATTTAAATTTTTTGACAGACCAGACCTATTTAAACAAAGCATTTCTAAACATGGATATTATGTAAACCATGTTTTTTGGGAAAATTCTATGGTGGTCCACTTAGATAGGTGTCCACGGTGGATCAACGCTTGAAAATAACCTGAAACTATGACTCATAGTACTTGTTGGAAGCAGAAACTCACGATCCTCTTTGTCTTAATGAGTGAGACGGTGACTCGATTGGTACTAGTTCTAAAAAACATCTGGGAAAACAAACGAGTCAACAAAACTCAACTCATTCGTTTTCCTTTGTTTTGATAGAACACACACCAATCAAGTCACCGTATCACCTATAACAGCAACAATGACTGTGATTTTCTACTTCCAAGAGCGACATCGGTTGTTACTTGTTATTGGTATCTCTACGATAATTTTCAAGTGGTCAACCGTAGAACCAGCCTGTTTTTTTGGTGAGAAAAGTTTAATTTCTTACAGTAACTAAGTCACTAGCACAATTGCAGAATTCAACTGTATTCCCACTTGGGGAACAACCACGGAGTGCTGTAACATCGACACAATCCGTTAAACTCCTCACAAGAATGGAACAACTGAGACTGTTAACCAAGGCAGAGAAAGCAGGTTTATTATCAGCCGCCGAGAAAGCAGGATTATCGCTATCAACAATAGAGAAACTTGGTCTCCTCTCAAAAGCAGAAGAACTCGGTGTTTTATCAGCTGCTACTGATCCATCAACTCCAGGATCACTCTTCACACTCAGCTTTGTTTTACTTCTTTTAGGTCCTTTGTTTGTTTATCTTGTTCCCGAAGATAATGTTGTCGAGGTTGGATTACAGGCTGTGGTTGCTTTGGTCTGTGTTGTTGGTGGTTCTGCTGGTTTTGCTGGTTCAAGTTTTGTATCCAACTTGCAGAAAATAAAATAA

Protein sequence

>MS.gene79400.t1

MMMTMKASASICSNSLIFYSSPSTQNSHTFSPFLVKSMATPKPVPSVSKTVASKKNSTVFPLGEQPRSAVTSTQSVKLLTRMEQLRLLTKAEKAGLLSAAEKAGLSLSTIEKLGLLSKAEELGVLSAATDPSTPGSLFTLSFVLLLLGPLFVYLVPEDNVVEVGLQAVVALVCVVGGSAGFAGSSFVSNLQKIK