AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71666


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71666.t1 MTR_1g107305 84.536 194 2 2 1 166 107 300 4.50e-110 318
MS.gene71666.t1 MTR_1g107295 83.505 194 4 2 1 166 107 300 3.13e-105 311
MS.gene71666.t1 MTR_4g062510 77.835 194 15 2 1 166 106 299 6.83e-92 277
MS.gene71666.t1 MTR_4g062500 65.285 193 39 2 1 166 104 295 2.50e-78 242
MS.gene71666.t1 MTR_7g028020 41.667 192 75 4 2 166 102 283 1.17e-37 135
MS.gene71666.t1 MTR_7g027960 41.146 192 76 4 2 166 102 283 4.26e-37 133
MS.gene71666.t1 MTR_3g020780 35.484 186 91 2 8 166 111 294 3.05e-35 128
MS.gene71666.t1 MTR_7g092620 38.068 176 78 3 2 150 104 275 3.97e-34 125
MS.gene71666.t1 MTR_4g062530 38.860 193 71 5 4 166 107 282 2.02e-33 122
MS.gene71666.t1 MTR_0181s0030 35.789 190 87 6 4 166 106 287 5.10e-29 111
MS.gene71666.t1 MTR_0171s0020 31.959 194 98 3 1 166 106 293 3.80e-28 108
MS.gene71666.t1 MTR_0171s0010 32.474 194 97 3 1 166 106 293 1.10e-27 107
MS.gene71666.t1 MTR_7g056103 33.333 189 92 4 3 164 105 286 4.14e-26 103
MS.gene71666.t1 MTR_8g036185 30.769 182 98 2 8 162 111 291 1.04e-25 102
MS.gene71666.t1 MTR_0171s0030 28.796 191 102 3 4 166 21 205 9.08e-24 96.3
MS.gene71666.t1 MTR_8g468780 29.534 193 98 6 4 166 38 222 8.72e-21 88.2
MS.gene71666.t1 MTR_4g088160 29.189 185 86 5 4 158 109 278 1.57e-20 87.8
MS.gene71666.t1 MTR_4g088195 27.461 193 95 5 4 166 109 286 4.91e-19 83.6
MS.gene71666.t1 MTR_8g469300 27.461 193 102 6 4 166 107 291 6.76e-18 80.1
MS.gene71666.t1 MTR_4g088170 26.425 193 97 5 4 166 109 286 1.74e-17 79.0
MS.gene71666.t1 MTR_1g442730 27.273 187 105 3 3 166 106 284 1.84e-16 75.9
MS.gene71666.t1 MTR_3g057980 26.257 179 100 4 4 157 109 280 1.59e-14 70.5
MS.gene71666.t1 MTR_1g035190 25.806 186 105 6 3 164 92 268 1.63e-14 70.5
MS.gene71666.t1 MTR_1g035140 26.115 157 91 2 3 136 109 263 1.66e-14 70.5
MS.gene71666.t1 MTR_7g092600 30.556 180 96 5 10 166 117 290 2.94e-14 69.7
MS.gene71666.t1 MTR_3g076550 29.730 185 92 7 4 159 108 283 4.30e-14 69.3
MS.gene71666.t1 MTR_3g058000 29.143 175 92 5 7 157 114 280 6.39e-14 68.6
MS.gene71666.t1 MTR_3g025230 26.623 154 81 2 8 129 108 261 8.47e-14 68.6
MS.gene71666.t1 MTR_5g018980 27.072 181 103 4 4 159 109 285 9.37e-14 68.2
MS.gene71666.t1 MTR_3g024520 26.623 154 81 2 8 129 108 261 1.22e-13 67.8
MS.gene71666.t1 MTR_3g058240 25.568 176 100 4 7 158 77 245 1.41e-13 67.8
MS.gene71666.t1 MTR_0554s0020 27.097 155 85 4 3 132 109 260 5.55e-13 65.9
MS.gene71666.t1 MTR_1g023700 34.524 84 55 0 3 86 105 188 5.78e-13 65.9
MS.gene71666.t1 MTR_3g058380 24.022 179 104 4 3 157 28 198 7.32e-13 65.5
MS.gene71666.t1 MTR_6g035115 31.481 108 68 2 3 110 109 210 8.69e-13 65.5
MS.gene71666.t1 MTR_3g058230 24.581 179 101 5 3 157 37 205 2.28e-12 64.3
MS.gene71666.t1 MTR_3g058210 26.257 179 101 6 3 157 109 280 3.61e-12 63.5
MS.gene71666.t1 MTR_5g019010 26.519 181 103 3 3 159 108 282 8.21e-12 62.8
MS.gene71666.t1 MTR_3g057900 25.843 178 102 5 4 157 109 280 8.50e-12 62.8
MS.gene71666.t1 MTR_8g012585 24.479 192 101 6 8 164 50 232 1.16e-11 62.0
MS.gene71666.t1 MTR_1g023730 30.952 84 58 0 3 86 104 187 1.19e-11 62.0
MS.gene71666.t1 MTR_3g058400 29.452 146 98 3 2 144 108 251 1.19e-11 62.0
MS.gene71666.t1 MTR_3g436540 27.517 149 80 3 8 128 116 264 1.22e-11 62.0
MS.gene71666.t1 MTR_5g095260 32.609 92 58 2 2 92 112 200 1.96e-11 61.6
MS.gene71666.t1 MTR_5g018990 26.230 183 99 4 4 159 110 283 3.20e-11 60.8
MS.gene71666.t1 MTR_3g058250 24.022 179 105 4 3 157 107 278 3.49e-11 60.8
MS.gene71666.t1 MTR_6g084760 29.268 82 58 0 8 89 1 82 3.82e-11 57.4
MS.gene71666.t1 MTR_4g104640 25.946 185 100 4 3 159 121 296 3.83e-11 60.8
MS.gene71666.t1 MTR_3g057970 31.461 89 59 1 4 92 106 192 6.34e-11 60.1
MS.gene71666.t1 MTR_3g058390 25.517 145 103 3 3 144 107 249 7.45e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71666.t1 AT5G06900 28.272 191 97 4 5 166 107 286 3.87e-22 92.4
MS.gene71666.t1 AT3G20940 30.270 185 90 2 10 166 121 294 1.46e-21 90.9
MS.gene71666.t1 AT3G20950 33.526 173 99 4 10 166 125 297 1.88e-21 90.5
MS.gene71666.t1 AT2G14100 29.730 185 90 2 10 166 122 294 1.13e-20 88.2
MS.gene71666.t1 AT2G27000 30.978 184 86 3 13 166 119 291 1.53e-20 87.8
MS.gene71666.t1 AT2G42250 31.522 184 89 3 10 166 119 292 4.13e-20 86.7
MS.gene71666.t1 AT3G20080 33.103 145 69 1 14 130 125 269 2.20e-19 84.3
MS.gene71666.t1 AT3G20080 33.103 145 69 1 14 130 125 269 2.20e-19 84.3
MS.gene71666.t1 AT1G50520 28.351 194 99 2 1 166 115 296 2.40e-19 84.3
MS.gene71666.t1 AT1G50560 31.351 185 87 3 10 166 125 297 4.93e-19 83.6
MS.gene71666.t1 AT5G42580 32.374 139 78 1 8 130 116 254 2.55e-18 81.3
MS.gene71666.t1 AT5G47990 28.571 182 91 2 13 166 124 294 4.32e-18 80.9
MS.gene71666.t1 AT5G06905 28.108 185 97 3 9 166 108 283 1.11e-17 79.7
MS.gene71666.t1 AT4G15350 40.000 95 57 0 14 108 118 212 1.24e-17 79.3
MS.gene71666.t1 AT3G20090 26.344 186 96 3 10 166 122 295 1.56e-17 79.3
MS.gene71666.t1 AT3G20110 29.508 183 89 2 12 166 120 290 1.27e-16 76.6
MS.gene71666.t1 AT3G20130 33.333 114 76 0 8 121 91 204 1.33e-16 76.6
MS.gene71666.t1 AT3G20130 33.333 114 76 0 8 121 119 232 1.67e-16 76.3
MS.gene71666.t1 AT4G15360 26.554 177 92 3 18 166 127 293 3.05e-16 75.5
MS.gene71666.t1 AT3G20100 27.568 185 95 2 10 166 120 293 7.28e-16 74.3
MS.gene71666.t1 AT3G32047 24.865 185 98 2 10 166 121 292 9.10e-16 73.9
MS.gene71666.t1 AT2G05180 40.659 91 54 0 18 108 128 218 2.27e-15 72.8
MS.gene71666.t1 AT5G25140 35.052 97 61 1 8 102 105 201 6.23e-15 71.6
MS.gene71666.t1 AT3G20120 40.625 96 57 0 13 108 123 218 1.32e-14 70.9
MS.gene71666.t1 AT3G20140 28.750 160 84 2 13 142 122 281 2.39e-14 70.1
MS.gene71666.t1 AT4G13310 29.379 177 83 5 16 163 116 279 4.09e-14 69.3
MS.gene71666.t1 AT4G13310 29.379 177 83 5 16 163 116 279 5.13e-14 68.9
MS.gene71666.t1 AT3G20960 25.668 187 100 2 8 166 15 190 5.45e-14 68.9
MS.gene71666.t1 AT3G26300 26.012 173 110 3 8 163 107 278 7.47e-14 68.6
MS.gene71666.t1 AT3G20960 25.668 187 100 2 8 166 118 293 7.85e-14 68.6
MS.gene71666.t1 AT3G48320 28.049 164 106 3 10 163 110 271 2.24e-13 67.0
MS.gene71666.t1 AT4G15330 29.143 175 108 5 8 166 87 261 2.84e-13 67.0
MS.gene71666.t1 AT4G15330 29.143 175 108 5 8 166 115 289 2.94e-13 67.0
MS.gene71666.t1 AT3G26310 24.855 173 112 3 8 163 106 277 3.13e-12 63.9
MS.gene71666.t1 AT4G15380 35.714 98 56 2 13 108 122 214 1.47e-11 62.0
MS.gene71666.t1 AT4G13290 25.989 177 89 4 16 163 117 280 3.46e-11 60.8
MS.gene71666.t1 AT3G20080 29.545 132 65 1 27 130 1 132 3.70e-11 60.8
MS.gene71666.t1 AT3G20080 29.545 132 65 1 27 130 1 132 3.70e-11 60.8
MS.gene71666.t1 AT3G20080 29.545 132 65 1 27 130 1 132 3.70e-11 60.8
MS.gene71666.t1 AT3G20080 29.545 132 65 1 27 130 1 132 3.70e-11 60.8
MS.gene71666.t1 AT3G48310 26.506 166 110 3 8 163 108 271 4.19e-11 60.5
MS.gene71666.t1 AT5G25180 29.897 97 66 1 8 102 105 201 4.99e-11 60.5

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGTTTGAATGAATTCA+TGG 0.254720 1.1:+76980946 None:intergenic
ATAATCAAGCAGAACAAGTT+AGG 0.294081 1.1:-76980815 MS.gene71666:CDS
GAATGAATTCATGGAGTTCT+TGG 0.307299 1.1:+76980955 None:intergenic
AACTTTGGCAAATAAGTCTA+AGG 0.336693 1.1:-76980931 MS.gene71666:CDS
CCATGAATTCATTCAAACTT+TGG 0.338713 1.1:-76980946 MS.gene71666:CDS
TGTGAGGTTCACACTTTCTT+CGG 0.366286 1.1:+76980908 None:intergenic
CATCCGTGATATTATGTTGT+TGG 0.377371 1.1:+76980866 None:intergenic
GCTCAGCACCACTGAGCTCT+TGG 0.389280 1.1:-76981015 MS.gene71666:CDS
AATGAATTCATGGAGTTCTT+GGG 0.401039 1.1:+76980956 None:intergenic
CAAAAGGTATGATGCTTTGT+TGG 0.407133 1.1:-76980679 MS.gene71666:CDS
ATCATAGGCAACTACGTTGA+TGG 0.425364 1.1:+76981091 None:intergenic
TCGCCCCTTATGGGACGTAT+TGG 0.425739 1.1:-76981052 MS.gene71666:CDS
ATTTCCAATACGTCCCATAA+GGG 0.429868 1.1:+76981048 None:intergenic
AATTTCCAATACGTCCCATA+AGG 0.453123 1.1:+76981047 None:intergenic
ATAGATGGTGTTGGTGAGAA+TGG 0.474210 1.1:-76980609 MS.gene71666:CDS
AGGGAAGCCAAGCTAATAGA+TGG 0.474398 1.1:-76980624 MS.gene71666:CDS
ACCAACACCATCTATTAGCT+TGG 0.489140 1.1:+76980617 None:intergenic
AGCAAAAGTTGCATCATCAT+AGG 0.489946 1.1:+76981076 None:intergenic
CTGATCGTGAAGAACTAAGA+AGG 0.497144 1.1:-76980644 MS.gene71666:CDS
CTCAGCACCACTGAGCTCTT+GGG 0.498900 1.1:-76981014 MS.gene71666:CDS
TAATCAAGCAGAACAAGTTA+GGG 0.511063 1.1:-76980814 MS.gene71666:CDS
TGGAGTTCTTGGGTTCGAAT+CGG 0.519216 1.1:+76980966 None:intergenic
AGCAGAACAAGTTAGGGCAT+TGG 0.520526 1.1:-76980808 MS.gene71666:CDS
GGGCCTTGGACATACACAAA+AGG 0.575364 1.1:-76980695 MS.gene71666:CDS
TGATCGTGAAGAACTAAGAA+GGG 0.579644 1.1:-76980643 MS.gene71666:CDS
ATGTTGAGTATTGATTGCTC+AGG 0.600833 1.1:-76980843 MS.gene71666:CDS
GCTTTATCAAAGCTTGTGTG+AGG 0.613323 1.1:+76980892 None:intergenic
TTTCCAATACGTCCCATAAG+GGG 0.615196 1.1:+76981049 None:intergenic
TTTCCAACAACATAATATCA+CGG 0.620811 1.1:-76980869 MS.gene71666:CDS
GCCAAGCTAATAGATGGTGT+TGG 0.622063 1.1:-76980618 MS.gene71666:CDS
AGTTAGGGCATTGGTTCGCG+AGG 0.647937 1.1:-76980799 MS.gene71666:intron
AATCGGTAGAAATTGCGACA+TGG 0.690545 1.1:+76980983 None:intergenic
TCGATTACCCAAGAGCTCAG+TGG 0.718966 1.1:+76981007 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATGTTTCAGATTTTAT+AGG - chr1.1:76980890-76980909 MS.gene71666:CDS 15.0%
! TTTCCAACAACATAATATCA+CGG - chr1.1:76980777-76980796 MS.gene71666:intron 25.0%
AACTTTGGCAAATAAGTCTA+AGG - chr1.1:76980715-76980734 MS.gene71666:intron 30.0%
AATGAATTCATGGAGTTCTT+GGG + chr1.1:76980693-76980712 None:intergenic 30.0%
ATAATCAAGCAGAACAAGTT+AGG - chr1.1:76980831-76980850 MS.gene71666:CDS 30.0%
CCAAAGTTTGAATGAATTCA+TGG + chr1.1:76980703-76980722 None:intergenic 30.0%
CCATGAATTCATTCAAACTT+TGG - chr1.1:76980700-76980719 MS.gene71666:intron 30.0%
TAATCAAGCAGAACAAGTTA+GGG - chr1.1:76980832-76980851 MS.gene71666:CDS 30.0%
!!! ACTTGCAAGGTTTTAAAAAG+AGG - chr1.1:76980930-76980949 MS.gene71666:CDS 30.0%
!!! CTTGCAAGGTTTTAAAAAGA+GGG - chr1.1:76980931-76980950 MS.gene71666:CDS 30.0%
AATTTCCAATACGTCCCATA+AGG + chr1.1:76980602-76980621 None:intergenic 35.0%
AGCAAAAGTTGCATCATCAT+AGG + chr1.1:76980573-76980592 None:intergenic 35.0%
ATTTCCAATACGTCCCATAA+GGG + chr1.1:76980601-76980620 None:intergenic 35.0%
CATCCGTGATATTATGTTGT+TGG + chr1.1:76980783-76980802 None:intergenic 35.0%
GAATGAATTCATGGAGTTCT+TGG + chr1.1:76980694-76980713 None:intergenic 35.0%
TGATCGTGAAGAACTAAGAA+GGG - chr1.1:76981003-76981022 MS.gene71666:CDS 35.0%
! ATACCTTTTGTGTATGTCCA+AGG + chr1.1:76980957-76980976 None:intergenic 35.0%
! CAAAAGGTATGATGCTTTGT+TGG - chr1.1:76980967-76980986 MS.gene71666:CDS 35.0%
!! ATGTTGAGTATTGATTGCTC+AGG - chr1.1:76980803-76980822 MS.gene71666:CDS 35.0%
!! TTCAAGAACTTTGACTTGCA+AGG - chr1.1:76980917-76980936 MS.gene71666:CDS 35.0%
AATCGGTAGAAATTGCGACA+TGG + chr1.1:76980666-76980685 None:intergenic 40.0%
ACCAACACCATCTATTAGCT+TGG + chr1.1:76981032-76981051 None:intergenic 40.0%
ATCATAGGCAACTACGTTGA+TGG + chr1.1:76980558-76980577 None:intergenic 40.0%
CTGATCGTGAAGAACTAAGA+AGG - chr1.1:76981002-76981021 MS.gene71666:CDS 40.0%
GCTTTATCAAAGCTTGTGTG+AGG + chr1.1:76980757-76980776 None:intergenic 40.0%
TGTGAGGTTCACACTTTCTT+CGG + chr1.1:76980741-76980760 None:intergenic 40.0%
TTTCCAATACGTCCCATAAG+GGG + chr1.1:76980600-76980619 None:intergenic 40.0%
! CGCGAGGTAACACAAATTTT+TGG - chr1.1:76980863-76980882 MS.gene71666:CDS 40.0%
!! ATAGATGGTGTTGGTGAGAA+TGG - chr1.1:76981037-76981056 MS.gene71666:CDS 40.0%
!!! AGGTTTTAAAAAGAGGGCCT+TGG - chr1.1:76980937-76980956 MS.gene71666:CDS 40.0%
AGCAGAACAAGTTAGGGCAT+TGG - chr1.1:76980838-76980857 MS.gene71666:CDS 45.0%
AGGGAAGCCAAGCTAATAGA+TGG - chr1.1:76981022-76981041 MS.gene71666:CDS 45.0%
GCCAAGCTAATAGATGGTGT+TGG - chr1.1:76981028-76981047 MS.gene71666:CDS 45.0%
TGGAGTTCTTGGGTTCGAAT+CGG + chr1.1:76980683-76980702 None:intergenic 45.0%
! ACTTTTGCTTTCGCCCCTTA+TGG - chr1.1:76980584-76980603 MS.gene71666:CDS 45.0%
! CTTTTGCTTTCGCCCCTTAT+GGG - chr1.1:76980585-76980604 MS.gene71666:CDS 45.0%
GGGCCTTGGACATACACAAA+AGG - chr1.1:76980951-76980970 MS.gene71666:CDS 50.0%
TCGATTACCCAAGAGCTCAG+TGG + chr1.1:76980642-76980661 None:intergenic 50.0%
CTCAGCACCACTGAGCTCTT+GGG - chr1.1:76980632-76980651 MS.gene71666:CDS 55.0%
TCGCCCCTTATGGGACGTAT+TGG - chr1.1:76980594-76980613 MS.gene71666:CDS 55.0%
!! AGTTAGGGCATTGGTTCGCG+AGG - chr1.1:76980847-76980866 MS.gene71666:CDS 55.0%
GCTCAGCACCACTGAGCTCT+TGG - chr1.1:76980631-76980650 MS.gene71666:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 76980545 76981123 76980545 ID=MS.gene71666
chr1.1 mRNA 76980545 76981123 76980545 ID=MS.gene71666.t1;Parent=MS.gene71666
chr1.1 exon 76980800 76981123 76980800 ID=MS.gene71666.t1.exon1;Parent=MS.gene71666.t1
chr1.1 CDS 76980800 76981123 76980800 ID=cds.MS.gene71666.t1;Parent=MS.gene71666.t1
chr1.1 exon 76980545 76980718 76980545 ID=MS.gene71666.t1.exon2;Parent=MS.gene71666.t1
chr1.1 CDS 76980545 76980718 76980545 ID=cds.MS.gene71666.t1;Parent=MS.gene71666.t1
Gene Sequence

>MS.gene71666

ATGAGCATAGCCATCAACGTAGTTGCCTATGATGATGCAACTTTTGCTTTCGCCCCTTATGGGACGTATTGGAAATTTATTAAAAAGCTCAGCACCACTGAGCTCTTGGGTAATCGAACCATGTCGCAATTTCTACCGATTCGAACCCAAGAACTCCATGAATTCATTCAAACTTTGGCAAATAAGTCTAAGGCCGAAGAAAGTGTGAACCTCACACAAGCTTTGATAAAGCTTTCCAACAACATAATATCACGGATGATGTTGAGTATTGATTGCTCAGGAACAGATAATCAAGCAGAACAAGTTAGGGCATTGGTTCGCGAGGTAACACAAATTTTTGGAGAATTTAATGTTTCAGATTTTATAGGAATTTTCAAGAACTTTGACTTGCAAGGTTTTAAAAAGAGGGCCTTGGACATACACAAAAGGTATGATGCTTTGTTGGATAAGATTATTTCTGATCGTGAAGAACTAAGAAGGGAAGCCAAGCTAATAGATGGTGTTGGTGAGAATGGTGAAGAGAGACTGAAAGATTTTCTTGACATTTTGCTAGATGTCTACAGTGAGAAAGACCGTGAA

Protein sequence

>MS.gene71666.t1

MSIAINVVAYDDATFAFAPYGTYWKFIKKLSTTELLGNRTMSQFLPIRTQELHEFIQTLANKSKAEESVNLTQALIKLSNNIISRMMLSIDCSGTDNQAEQVRALVRERALDIHKRYDALLDKIISDREELRREAKLIDGVGENGEERLKDFLDILLDVYSEKDRE