Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88978.t1 | PNY14915.1 | 85.9 | 306 | 34 | 3 | 4 | 304 | 3 | 304 | 4.80E-143 | 517.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88978.t1 | Q9SGS4 | 64.8 | 304 | 91 | 5 | 10 | 304 | 6 | 302 | 2.1e-102 | 373.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88978.t1 | A0A2K3PI43 | 85.9 | 306 | 34 | 3 | 4 | 304 | 3 | 304 | 3.5e-143 | 517.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene88978 | 0.82693 | 2.07E-54 | -1.69E-46 |
| MS.gene049170 | MS.gene88978 | 0.803285 | 3.75E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene88978 | 0.802694 | 4.97E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene88978 | 0.835843 | 1.32E-56 | -1.69E-46 |
| MS.gene049369 | MS.gene88978 | 0.817128 | 3.87E-52 | -1.69E-46 |
| MS.gene049488 | MS.gene88978 | 0.836625 | 8.39E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene88978 | 0.849374 | 3.37E-60 | -1.69E-46 |
| MS.gene049805 | MS.gene88978 | 0.831151 | 1.96E-55 | -1.69E-46 |
| MS.gene050060 | MS.gene88978 | 0.801475 | 8.85E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene88978 | 0.804001 | 2.66E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene88978 | 0.833089 | 6.51E-56 | -1.69E-46 |
| MS.gene050400 | MS.gene88978 | 0.868446 | 6.49E-66 | -1.69E-46 |
| MS.gene050426 | MS.gene88978 | -0.843425 | 1.41E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene88978 | 0.815024 | 1.14E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene88978 | 0.807546 | 4.80E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene88978 | 0.866774 | 2.23E-65 | -1.69E-46 |
| MS.gene050625 | MS.gene88978 | 0.816221 | 6.18E-52 | -1.69E-46 |
| MS.gene050670 | MS.gene88978 | 0.909009 | 9.14E-82 | -1.69E-46 |
| MS.gene050747 | MS.gene88978 | 0.818375 | 2.03E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene88978 | 0.83837 | 2.99E-57 | -1.69E-46 |
| MS.gene05081 | MS.gene88978 | 0.820466 | 6.76E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene88978 | 0.816464 | 5.46E-52 | -1.69E-46 |
| MS.gene050971 | MS.gene88978 | 0.814071 | 1.86E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene88978 | 0.890266 | 1.15E-73 | -1.69E-46 |
| MS.gene051360 | MS.gene88978 | 0.805706 | 1.17E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene88978 | 0.875379 | 3.23E-68 | -1.69E-46 |
| MS.gene051438 | MS.gene88978 | 0.800483 | 1.41E-48 | -1.69E-46 |
| MS.gene051525 | MS.gene88978 | 0.909869 | 3.53E-82 | -1.69E-46 |
| MS.gene051582 | MS.gene88978 | 0.854778 | 9.81E-62 | -1.69E-46 |
| MS.gene051588 | MS.gene88978 | 0.810299 | 1.23E-50 | -1.69E-46 |
| MS.gene051605 | MS.gene88978 | 0.805115 | 1.56E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene88978 | 0.811517 | 6.73E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene88978 | 0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene88978 | 0.830118 | 3.51E-55 | -1.69E-46 |
| MS.gene051941 | MS.gene88978 | 0.802808 | 4.71E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene88978 | -0.872349 | 3.40E-67 | -1.69E-46 |
| MS.gene052077 | MS.gene88978 | 0.809592 | 1.75E-50 | -1.69E-46 |
| MS.gene052128 | MS.gene88978 | -0.847327 | 1.24E-59 | -1.69E-46 |
| MS.gene052135 | MS.gene88978 | 0.802515 | 5.41E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene88978 | 0.847439 | 1.16E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene88978 | 0.866959 | 1.95E-65 | -1.69E-46 |
| MS.gene052310 | MS.gene88978 | 0.855864 | 4.74E-62 | -1.69E-46 |
| MS.gene052322 | MS.gene88978 | 0.858418 | 8.35E-63 | -1.69E-46 |
| MS.gene052340 | MS.gene88978 | 0.856344 | 3.43E-62 | -1.69E-46 |
| MS.gene052346 | MS.gene88978 | 0.869454 | 3.06E-66 | -1.69E-46 |
| MS.gene052414 | MS.gene88978 | 0.84543 | 4.08E-59 | -1.69E-46 |
| MS.gene052421 | MS.gene88978 | 0.871766 | 5.32E-67 | -1.69E-46 |
| MS.gene052469 | MS.gene88978 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene88978 | 0.854826 | 9.51E-62 | -1.69E-46 |
| MS.gene052484 | MS.gene88978 | 0.801807 | 7.56E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene88978 | 0.862725 | 4.13E-64 | -1.69E-46 |
| MS.gene052501 | MS.gene88978 | 0.855144 | 7.68E-62 | -1.69E-46 |
| MS.gene052505 | MS.gene88978 | 0.851476 | 8.66E-61 | -1.69E-46 |
| MS.gene052615 | MS.gene88978 | 0.822621 | 2.15E-53 | -1.69E-46 |
| MS.gene052982 | MS.gene88978 | 0.853963 | 1.69E-61 | -1.69E-46 |
| MS.gene052999 | MS.gene88978 | 0.838302 | 3.12E-57 | -1.69E-46 |
| MS.gene053103 | MS.gene88978 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene053169 | MS.gene88978 | 0.866058 | 3.76E-65 | -1.69E-46 |
| MS.gene053207 | MS.gene88978 | 0.804664 | 1.94E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene88978 | 0.821806 | 3.32E-53 | -1.69E-46 |
| MS.gene05335 | MS.gene88978 | 0.831586 | 1.53E-55 | -1.69E-46 |
| MS.gene053436 | MS.gene88978 | 0.807937 | 3.96E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene88978 | 0.850869 | 1.28E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene88978 | 0.809273 | 2.05E-50 | -1.69E-46 |
| MS.gene053758 | MS.gene88978 | 0.825167 | 5.43E-54 | -1.69E-46 |
| MS.gene053813 | MS.gene88978 | 0.80557 | 1.25E-49 | -1.69E-46 |
| MS.gene053873 | MS.gene88978 | 0.829413 | 5.22E-55 | -1.69E-46 |
| MS.gene053912 | MS.gene88978 | 0.833089 | 6.52E-56 | -1.69E-46 |
| MS.gene053928 | MS.gene88978 | 0.820455 | 6.80E-53 | -1.69E-46 |
| MS.gene054010 | MS.gene88978 | 0.801937 | 7.11E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene88978 | 0.82627 | 2.97E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene88978 | 0.820686 | 6.02E-53 | -1.69E-46 |
| MS.gene054349 | MS.gene88978 | 0.801239 | 9.89E-49 | -1.69E-46 |
| MS.gene054556 | MS.gene88978 | 0.86276 | 4.03E-64 | -1.69E-46 |
| MS.gene054719 | MS.gene88978 | 0.813768 | 2.17E-51 | -1.69E-46 |
| MS.gene054742 | MS.gene88978 | -0.804213 | 2.41E-49 | -1.69E-46 |
| MS.gene054917 | MS.gene88978 | 0.803924 | 2.76E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene88978 | 0.801511 | 8.70E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene88978 | 0.802006 | 6.88E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene88978 | 0.834296 | 3.25E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene88978 | 0.893319 | 7.02E-75 | -1.69E-46 |
| MS.gene055206 | MS.gene88978 | 0.811859 | 5.67E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene88978 | 0.816636 | 4.99E-52 | -1.69E-46 |
| MS.gene055369 | MS.gene88978 | 0.840749 | 7.21E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene88978 | 0.810497 | 1.12E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene88978 | 0.854351 | 1.30E-61 | -1.69E-46 |
| MS.gene05553 | MS.gene88978 | 0.858964 | 5.74E-63 | -1.69E-46 |
| MS.gene05556 | MS.gene88978 | 0.821567 | 3.77E-53 | -1.69E-46 |
| MS.gene055722 | MS.gene88978 | 0.816643 | 4.98E-52 | -1.69E-46 |
| MS.gene055741 | MS.gene88978 | 0.870699 | 1.20E-66 | -1.69E-46 |
| MS.gene055744 | MS.gene88978 | 0.876677 | 1.15E-68 | -1.69E-46 |
| MS.gene055909 | MS.gene88978 | 0.843225 | 1.60E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene88978 | 0.848921 | 4.50E-60 | -1.69E-46 |
| MS.gene05592 | MS.gene88978 | 0.806297 | 8.81E-50 | -1.69E-46 |
| MS.gene05594 | MS.gene88978 | 0.80954 | 1.80E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene88978 | 0.835592 | 1.53E-56 | -1.69E-46 |
| MS.gene056337 | MS.gene88978 | 0.804186 | 2.44E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene88978 | 0.855949 | 4.47E-62 | -1.69E-46 |
| MS.gene056511 | MS.gene88978 | 0.871993 | 4.47E-67 | -1.69E-46 |
| MS.gene056514 | MS.gene88978 | 0.86182 | 7.84E-64 | -1.69E-46 |
| MS.gene05664 | MS.gene88978 | 0.81913 | 1.37E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene88978 | 0.830324 | 3.13E-55 | -1.69E-46 |
| MS.gene05666 | MS.gene88978 | 0.811278 | 7.58E-51 | -1.69E-46 |
| MS.gene056782 | MS.gene88978 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene056877 | MS.gene88978 | 0.853744 | 1.95E-61 | -1.69E-46 |
| MS.gene057019 | MS.gene88978 | 0.843513 | 1.34E-58 | -1.69E-46 |
| MS.gene05702 | MS.gene88978 | 0.836994 | 6.75E-57 | -1.69E-46 |
| MS.gene057076 | MS.gene88978 | 0.823318 | 1.48E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene88978 | 0.872825 | 2.36E-67 | -1.69E-46 |
| MS.gene057146 | MS.gene88978 | 0.801002 | 1.11E-48 | -1.69E-46 |
| MS.gene057196 | MS.gene88978 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene88978 | 0.841822 | 3.76E-58 | -1.69E-46 |
| MS.gene057332 | MS.gene88978 | 0.811955 | 5.40E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene88978 | 0.822704 | 2.05E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene88978 | 0.839977 | 1.15E-57 | -1.69E-46 |
| MS.gene057476 | MS.gene88978 | 0.836347 | 9.87E-57 | -1.69E-46 |
| MS.gene057734 | MS.gene88978 | 0.819536 | 1.10E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene88978 | 0.865498 | 5.65E-65 | -1.69E-46 |
| MS.gene057823 | MS.gene88978 | 0.866909 | 2.02E-65 | -1.69E-46 |
| MS.gene057825 | MS.gene88978 | 0.836577 | 8.62E-57 | -1.69E-46 |
| MS.gene057856 | MS.gene88978 | 0.806593 | 7.63E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene88978 | 0.841036 | 6.06E-58 | -1.69E-46 |
| MS.gene058006 | MS.gene88978 | 0.813792 | 2.14E-51 | -1.69E-46 |
| MS.gene058007 | MS.gene88978 | 0.863941 | 1.74E-64 | -1.69E-46 |
| MS.gene058019 | MS.gene88978 | 0.823855 | 1.11E-53 | -1.69E-46 |
| MS.gene058120 | MS.gene88978 | 0.806381 | 8.46E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene88978 | 0.802239 | 6.16E-49 | -1.69E-46 |
| MS.gene058376 | MS.gene88978 | 0.844889 | 5.71E-59 | -1.69E-46 |
| MS.gene058444 | MS.gene88978 | 0.875528 | 2.87E-68 | -1.69E-46 |
| MS.gene058660 | MS.gene88978 | 0.804683 | 1.92E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene88978 | 0.903629 | 2.85E-79 | -1.69E-46 |
| MS.gene058838 | MS.gene88978 | 0.810604 | 1.06E-50 | -1.69E-46 |
| MS.gene05883 | MS.gene88978 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene05886 | MS.gene88978 | 0.8509 | 1.26E-60 | -1.69E-46 |
| MS.gene058928 | MS.gene88978 | 0.826762 | 2.27E-54 | -1.69E-46 |
| MS.gene058948 | MS.gene88978 | 0.830261 | 3.24E-55 | -1.69E-46 |
| MS.gene058995 | MS.gene88978 | 0.823062 | 1.70E-53 | -1.69E-46 |
| MS.gene059011 | MS.gene88978 | 0.832074 | 1.16E-55 | -1.69E-46 |
| MS.gene05907 | MS.gene88978 | 0.841109 | 5.80E-58 | -1.69E-46 |
| MS.gene059080 | MS.gene88978 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene059082 | MS.gene88978 | 0.846279 | 2.40E-59 | -1.69E-46 |
| MS.gene059141 | MS.gene88978 | 0.852884 | 3.44E-61 | -1.69E-46 |
| MS.gene059366 | MS.gene88978 | 0.809586 | 1.76E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene88978 | 0.878409 | 2.88E-69 | -1.69E-46 |
| MS.gene059455 | MS.gene88978 | 0.828969 | 6.69E-55 | -1.69E-46 |
| MS.gene059462 | MS.gene88978 | 0.834933 | 2.25E-56 | -1.69E-46 |
| MS.gene059674 | MS.gene88978 | 0.81136 | 7.28E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene88978 | 0.839143 | 1.89E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene88978 | 0.86974 | 2.47E-66 | -1.69E-46 |
| MS.gene059692 | MS.gene88978 | 0.850436 | 1.70E-60 | -1.69E-46 |
| MS.gene059695 | MS.gene88978 | 0.857648 | 1.41E-62 | -1.69E-46 |
| MS.gene059711 | MS.gene88978 | 0.802761 | 4.81E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene88978 | 0.832625 | 8.50E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene88978 | 0.802108 | 6.56E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene88978 | 0.869003 | 4.29E-66 | -1.69E-46 |
| MS.gene060401 | MS.gene88978 | 0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene88978 | 0.897468 | 1.37E-76 | -1.69E-46 |
| MS.gene060469 | MS.gene88978 | 0.810696 | 1.01E-50 | -1.69E-46 |
| MS.gene060485 | MS.gene88978 | 0.822994 | 1.76E-53 | -1.69E-46 |
| MS.gene060804 | MS.gene88978 | 0.806247 | 9.02E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene88978 | 0.837085 | 6.40E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene88978 | 0.860886 | 1.51E-63 | -1.69E-46 |
| MS.gene06092 | MS.gene88978 | 0.816596 | 5.10E-52 | -1.69E-46 |
| MS.gene060986 | MS.gene88978 | 0.82225 | 2.62E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene88978 | 0.818484 | 1.91E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene88978 | 0.883742 | 3.47E-71 | -1.69E-46 |
| MS.gene061159 | MS.gene88978 | 0.845106 | 5.00E-59 | -1.69E-46 |
| MS.gene061214 | MS.gene88978 | 0.824598 | 7.40E-54 | -1.69E-46 |
| MS.gene061215 | MS.gene88978 | 0.824119 | 9.59E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88978.t1 | MTR_1g026125 | 90.365 | 301 | 16 | 4 | 10 | 304 | 5 | 298 | 0.0 | 534 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88978.t1 | AT1G76080 | 63.898 | 313 | 93 | 6 | 1 | 304 | 1 | 302 | 2.18e-136 | 388 |
Find 48 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGAGATATACCCTTTCT+TGG | 0.236936 | 1.4:-350357 | MS.gene88978:CDS |
| TGTGGTAGGTATGTTGGTTC+AGG | 0.274596 | 1.4:-349852 | MS.gene88978:CDS |
| TGTTGAGTTTCTTCTTGTTA+TGG | 0.292877 | 1.4:-350309 | MS.gene88978:CDS |
| GAGGGATATGGAAGTTGTTC+AGG | 0.305188 | 1.4:-349910 | MS.gene88978:CDS |
| GTTGAGTTTCTTCTTGTTAT+GGG | 0.323733 | 1.4:-350308 | MS.gene88978:CDS |
| TGAGGGTTTGCATATGAAAT+TGG | 0.324749 | 1.4:+350661 | None:intergenic |
| AGCTGCTGTGCCTGTTGCTT+TGG | 0.336628 | 1.4:+350511 | None:intergenic |
| GAATGAAGGGTAAGGAAGTT+AGG | 0.357510 | 1.4:+350614 | None:intergenic |
| AAGCTTATTGTTCTTGATGT+TGG | 0.379209 | 1.4:-350059 | MS.gene88978:CDS |
| TACTACGGTGATAACCATTC+AGG | 0.418710 | 1.4:-350146 | MS.gene88978:CDS |
| CATCGTTACATGTTCGGCTT+AGG | 0.421411 | 1.4:+350330 | None:intergenic |
| ACATTGTGGGCCATGTGTTA+AGG | 0.424051 | 1.4:-350030 | MS.gene88978:CDS |
| ACATCACCAACAAGCATGTC+TGG | 0.428494 | 1.4:+350173 | None:intergenic |
| GTGGTAGGTATGTTGGTTCA+GGG | 0.432018 | 1.4:-349851 | MS.gene88978:CDS |
| AACTCATTGGTGAAATTCTC+AGG | 0.435220 | 1.4:-349821 | MS.gene88978:CDS |
| ATTAGAGATGGTAAGATTTG+TGG | 0.439626 | 1.4:-349870 | MS.gene88978:CDS |
| GAAATTGAAGGAGGGATTGA+GGG | 0.440827 | 1.4:+350644 | None:intergenic |
| CGGCTTAGGTCAACCAAGAA+AGG | 0.444183 | 1.4:+350344 | None:intergenic |
| AAGATTTGTGGTAGGTATGT+TGG | 0.445880 | 1.4:-349858 | MS.gene88978:CDS |
| TTAGGATGAGTTGAAATTGA+AGG | 0.445917 | 1.4:+350632 | None:intergenic |
| TGAAATTGAAGGAGGGATTG+AGG | 0.455754 | 1.4:+350643 | None:intergenic |
| GTTGTTATCACCAAAGCAAC+AGG | 0.459253 | 1.4:-350521 | MS.gene88978:CDS |
| ACTCAACATCGTTACATGTT+CGG | 0.469979 | 1.4:+350324 | None:intergenic |
| ATTCATGAAGAAGAAGCTAT+TGG | 0.470180 | 1.4:-350197 | MS.gene88978:CDS |
| AAGAAGAACGAAGAGAATGA+AGG | 0.480454 | 1.4:+350600 | None:intergenic |
| AGGTATGTTGGTTCAGGGAA+AGG | 0.482216 | 1.4:-349846 | MS.gene88978:CDS |
| ATGTTGGTCTTAAACATTGT+GGG | 0.490241 | 1.4:-350043 | MS.gene88978:CDS |
| AGAAGAACGAAGAGAATGAA+GGG | 0.507243 | 1.4:+350601 | None:intergenic |
| AACGAAGAGAATGAAGGGTA+AGG | 0.514490 | 1.4:+350606 | None:intergenic |
| AGAACTCTGCAAAAGAGAAA+AGG | 0.515029 | 1.4:-350264 | MS.gene88978:CDS |
| GGATGAGTTGAAATTGAAGG+AGG | 0.516103 | 1.4:+350635 | None:intergenic |
| AATAAGTAACACGAACTCCT+TGG | 0.520146 | 1.4:+349796 | None:intergenic |
| TCAGGGAAAGGTGAACTCAT+TGG | 0.523543 | 1.4:-349834 | MS.gene88978:CDS |
| CTTGACAGCTTAATCACTGT+CGG | 0.540890 | 1.4:+350002 | None:intergenic |
| ATTGGTCCAGACATGCTTGT+TGG | 0.551371 | 1.4:-350179 | MS.gene88978:CDS |
| GATGAGTTGAAATTGAAGGA+GGG | 0.552894 | 1.4:+350636 | None:intergenic |
| AGATGTTGAGAAACTTATTG+AGG | 0.553117 | 1.4:-350099 | MS.gene88978:CDS |
| ATTCATTCTACTTCTCGGGT+AGG | 0.557229 | 1.4:-350557 | MS.gene88978:CDS |
| GGCTTAGGTCAACCAAGAAA+GGG | 0.570139 | 1.4:+350345 | None:intergenic |
| GATGTTGGTCTTAAACATTG+TGG | 0.582468 | 1.4:-350044 | MS.gene88978:CDS |
| GTTGGTGATGTGTTATACTA+CGG | 0.591943 | 1.4:-350161 | MS.gene88978:CDS |
| GTGAGTGATTGTAGTAGCCA+TGG | 0.593491 | 1.4:+350688 | None:intergenic |
| GAAGTTGCACTACACCTGAA+TGG | 0.604581 | 1.4:+350132 | None:intergenic |
| TGAGTGATTGTAGTAGCCAT+GGG | 0.617890 | 1.4:+350689 | None:intergenic |
| GAGATGGTAAGATTTGTGGT+AGG | 0.631571 | 1.4:-349866 | MS.gene88978:CDS |
| GTCGGATAAACCTTAACACA+TGG | 0.636213 | 1.4:+350020 | None:intergenic |
| GGTGAAATTCTCAGGTACCA+AGG | 0.658840 | 1.4:-349813 | MS.gene88978:CDS |
| CTCTTGTAGAAAGTGAAGTG+TGG | 0.692063 | 1.4:+350230 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAATTCATTCTACTTCTC+GGG | - | chr1.4:349919-349938 | MS.gene88978:CDS | 25.0% |
| ! | CAAAAATTCATTCTACTTCT+CGG | - | chr1.4:349918-349937 | MS.gene88978:CDS | 25.0% |
| ! | TCTCTAATGAACAAAAAAGT+AGG | + | chr1.4:350598-350617 | None:intergenic | 25.0% |
| !!! | ACTTTTTTGTTCATTAGAGA+TGG | - | chr1.4:350598-350617 | MS.gene88978:CDS | 25.0% |
| !!! | ATGATACTGTTATTTTTGCA+AGG | - | chr1.4:350509-350528 | MS.gene88978:CDS | 25.0% |
| AAGCTTATTGTTCTTGATGT+TGG | - | chr1.4:350421-350440 | MS.gene88978:CDS | 30.0% | |
| AGATGTTGAGAAACTTATTG+AGG | - | chr1.4:350381-350400 | MS.gene88978:CDS | 30.0% | |
| ATTAGAGATGGTAAGATTTG+TGG | - | chr1.4:350610-350629 | MS.gene88978:CDS | 30.0% | |
| ATTCATGAAGAAGAAGCTAT+TGG | - | chr1.4:350283-350302 | MS.gene88978:CDS | 30.0% | |
| TGTTGAGTTTCTTCTTGTTA+TGG | - | chr1.4:350171-350190 | MS.gene88978:CDS | 30.0% | |
| ! | ATAGTTGTATGCAGTTTTTG+AGG | - | chr1.4:350551-350570 | MS.gene88978:CDS | 30.0% |
| ! | CGAGAAGTAGAATGAATTTT+TGG | + | chr1.4:349920-349939 | None:intergenic | 30.0% |
| ! | GTTGAGTTTCTTCTTGTTAT+GGG | - | chr1.4:350172-350191 | MS.gene88978:CDS | 30.0% |
| ! | TAGTTGTATGCAGTTTTTGA+GGG | - | chr1.4:350552-350571 | MS.gene88978:CDS | 30.0% |
| ! | TTAGGATGAGTTGAAATTGA+AGG | + | chr1.4:349851-349870 | None:intergenic | 30.0% |
| !! | ATGTTGGTCTTAAACATTGT+GGG | - | chr1.4:350437-350456 | MS.gene88978:CDS | 30.0% |
| !!! | GTTATTTTTGCAAGGATGAA+TGG | - | chr1.4:350517-350536 | MS.gene88978:CDS | 30.0% |
| AACTCATTGGTGAAATTCTC+AGG | - | chr1.4:350659-350678 | MS.gene88978:CDS | 35.0% | |
| AAGAAGAACGAAGAGAATGA+AGG | + | chr1.4:349883-349902 | None:intergenic | 35.0% | |
| AAGATTTGTGGTAGGTATGT+TGG | - | chr1.4:350622-350641 | MS.gene88978:CDS | 35.0% | |
| AATAAGTAACACGAACTCCT+TGG | + | chr1.4:350687-350706 | None:intergenic | 35.0% | |
| ACTCAACATCGTTACATGTT+CGG | + | chr1.4:350159-350178 | None:intergenic | 35.0% | |
| AGAACTCTGCAAAAGAGAAA+AGG | - | chr1.4:350216-350235 | MS.gene88978:CDS | 35.0% | |
| AGAAGAACGAAGAGAATGAA+GGG | + | chr1.4:349882-349901 | None:intergenic | 35.0% | |
| CATTGAGATATACCCTTTCT+TGG | - | chr1.4:350123-350142 | MS.gene88978:CDS | 35.0% | |
| TGAGGGTTTGCATATGAAAT+TGG | + | chr1.4:349822-349841 | None:intergenic | 35.0% | |
| ! | GATGAGTTGAAATTGAAGGA+GGG | + | chr1.4:349847-349866 | None:intergenic | 35.0% |
| !! | GATGTTGGTCTTAAACATTG+TGG | - | chr1.4:350436-350455 | MS.gene88978:CDS | 35.0% |
| !! | GTTGGTGATGTGTTATACTA+CGG | - | chr1.4:350319-350338 | MS.gene88978:CDS | 35.0% |
| !! | TATGCAGTTTTTGAGGGATA+TGG | - | chr1.4:350558-350577 | MS.gene88978:CDS | 35.0% |
| AACGAAGAGAATGAAGGGTA+AGG | + | chr1.4:349877-349896 | None:intergenic | 40.0% | |
| ATTCATTCTACTTCTCGGGT+AGG | - | chr1.4:349923-349942 | MS.gene88978:CDS | 40.0% | |
| CTCTTGTAGAAAGTGAAGTG+TGG | + | chr1.4:350253-350272 | None:intergenic | 40.0% | |
| CTTGACAGCTTAATCACTGT+CGG | + | chr1.4:350481-350500 | None:intergenic | 40.0% | |
| GAAATTGAAGGAGGGATTGA+GGG | + | chr1.4:349839-349858 | None:intergenic | 40.0% | |
| GAATGAAGGGTAAGGAAGTT+AGG | + | chr1.4:349869-349888 | None:intergenic | 40.0% | |
| GAGATGGTAAGATTTGTGGT+AGG | - | chr1.4:350614-350633 | MS.gene88978:CDS | 40.0% | |
| GTCGGATAAACCTTAACACA+TGG | + | chr1.4:350463-350482 | None:intergenic | 40.0% | |
| GTTGTTATCACCAAAGCAAC+AGG | - | chr1.4:349959-349978 | MS.gene88978:CDS | 40.0% | |
| TACTACGGTGATAACCATTC+AGG | - | chr1.4:350334-350353 | MS.gene88978:CDS | 40.0% | |
| TGAAATTGAAGGAGGGATTG+AGG | + | chr1.4:349840-349859 | None:intergenic | 40.0% | |
| ! | GGATGAGTTGAAATTGAAGG+AGG | + | chr1.4:349848-349867 | None:intergenic | 40.0% |
| ACATCACCAACAAGCATGTC+TGG | + | chr1.4:350310-350329 | None:intergenic | 45.0% | |
| ACATTGTGGGCCATGTGTTA+AGG | - | chr1.4:350450-350469 | MS.gene88978:CDS | 45.0% | |
| ATTGGTCCAGACATGCTTGT+TGG | - | chr1.4:350301-350320 | MS.gene88978:CDS | 45.0% | |
| CATCGTTACATGTTCGGCTT+AGG | + | chr1.4:350153-350172 | None:intergenic | 45.0% | |
| GAAGTTGCACTACACCTGAA+TGG | + | chr1.4:350351-350370 | None:intergenic | 45.0% | |
| GAGGGATATGGAAGTTGTTC+AGG | - | chr1.4:350570-350589 | MS.gene88978:CDS | 45.0% | |
| GGCTTAGGTCAACCAAGAAA+GGG | + | chr1.4:350138-350157 | None:intergenic | 45.0% | |
| GGTGAAATTCTCAGGTACCA+AGG | - | chr1.4:350667-350686 | MS.gene88978:CDS | 45.0% | |
| TCAGGGAAAGGTGAACTCAT+TGG | - | chr1.4:350646-350665 | MS.gene88978:CDS | 45.0% | |
| !! | AGGTATGTTGGTTCAGGGAA+AGG | - | chr1.4:350634-350653 | MS.gene88978:CDS | 45.0% |
| !! | GTGGTAGGTATGTTGGTTCA+GGG | - | chr1.4:350629-350648 | MS.gene88978:CDS | 45.0% |
| !! | TGTGGTAGGTATGTTGGTTC+AGG | - | chr1.4:350628-350647 | MS.gene88978:CDS | 45.0% |
| CGGCTTAGGTCAACCAAGAA+AGG | + | chr1.4:350139-350158 | None:intergenic | 50.0% | |
| ! | AGCTGCTGTGCCTGTTGCTT+TGG | + | chr1.4:349972-349991 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 349794 | 350708 | 349794 | ID=MS.gene88978 |
| chr1.4 | mRNA | 349794 | 350708 | 349794 | ID=MS.gene88978.t1;Parent=MS.gene88978 |
| chr1.4 | exon | 349794 | 350708 | 349794 | ID=MS.gene88978.t1.exon1;Parent=MS.gene88978.t1 |
| chr1.4 | CDS | 349794 | 350708 | 349794 | ID=cds.MS.gene88978.t1;Parent=MS.gene88978.t1 |
| Gene Sequence |
| Protein sequence |
