AlfalfaGEDB | MS.gene062975

M. sativa cultivar XinJiangDaYe / MS.gene062975

KEGG: -
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062975.t1	XP_003607121.1	96.1	76	3	0	1	76	1	76	2.50E-32	148.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062975.t1	P33080	74.4	78	20	0	1	78	1	78	1.6e-27	123.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062975.t1	G7JM95	96.1	76	3	0	1	76	1	76	1.8e-32	148.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene062975	0.824218	9.09E-54	-1.69E-46
MS.gene050128	MS.gene062975	0.817751	2.80E-52	-1.69E-46
MS.gene052178	MS.gene062975	0.811328	7.40E-51	-1.69E-46
MS.gene054185	MS.gene062975	0.802029	6.81E-49	-1.69E-46
MS.gene055133	MS.gene062975	0.857548	1.51E-62	-1.69E-46
MS.gene055422	MS.gene062975	0.808092	3.67E-50	-1.69E-46
MS.gene057754	MS.gene062975	0.823719	1.19E-53	-1.69E-46
MS.gene057794	MS.gene062975	0.931286	4.73E-94	-1.69E-46
MS.gene057796	MS.gene062975	0.82151	3.88E-53	-1.69E-46
MS.gene059894	MS.gene062975	0.806085	9.76E-50	-1.69E-46
MS.gene06037	MS.gene062975	0.838972	2.09E-57	-1.69E-46
MS.gene061049	MS.gene062975	0.812773	3.58E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062975	MS.gene057790	PPI
MS.gene062975	MS.gene75315	PPI
MS.gene062975	MS.gene062952	PPI
MS.gene062975	MS.gene057818	PPI
MS.gene062975	MS.gene68164	PPI
MS.gene062975	MS.gene062979	PPI
MS.gene062975	MS.gene68759	PPI
MS.gene062975	MS.gene003586	PPI
MS.gene062975	MS.gene003627	PPI
MS.gene062975	MS.gene031200	PPI
MS.gene062975	MS.gene79888	PPI
MS.gene062975	MS.gene75676	PPI
MS.gene062975	MS.gene40326	PPI
MS.gene062975	MS.gene003590	PPI
MS.gene062975	MS.gene031199	PPI
MS.gene062975	MS.gene50687	PPI
MS.gene062975	MS.gene014223	PPI
MS.gene062975	MS.gene003625	PPI
MS.gene062975	MS.gene50766	PPI
MS.gene062975	MS.gene79885	PPI
MS.gene062975	MS.gene003587	PPI
MS.gene062975	MS.gene047517	PPI
MS.gene062975	MS.gene01358	PPI
MS.gene062975	MS.gene062963	PPI
MS.gene062975	MS.gene063112	PPI
MS.gene062975	MS.gene003602	PPI
MS.gene062975	MS.gene062984	PPI
MS.gene062975	MS.gene79889	PPI
MS.gene062975	MS.gene045368	PPI
MS.gene062975	MS.gene23370	PPI
MS.gene062975	MS.gene40327	PPI
MS.gene062975	MS.gene045360	PPI
MS.gene062975	MS.gene014222	PPI
MS.gene062975	MS.gene045361	PPI
MS.gene062975	MS.gene50659	PPI
MS.gene062975	MS.gene75678	PPI
MS.gene062975	MS.gene75314	PPI
MS.gene062975	MS.gene01764	PPI
MS.gene062975	MS.gene75673	PPI
MS.gene062975	MS.gene057778	PPI
MS.gene062975	MS.gene50763	PPI
MS.gene062975	MS.gene68760	PPI
MS.gene062975	MS.gene031198	PPI
MS.gene062975	MS.gene50676	PPI
MS.gene062975	MS.gene50764	PPI
MS.gene062975	MS.gene77528	PPI
MS.gene062975	MS.gene75316	PPI
MS.gene062975	MS.gene014671	PPI
MS.gene062975	MS.gene003615	PPI
MS.gene062975	MS.gene003577	PPI
MS.gene062975	MS.gene003594	PPI
MS.gene062975	MS.gene75672	PPI
MS.gene062975	MS.gene75674	PPI
MS.gene062975	MS.gene50654	PPI
MS.gene062975	MS.gene50675	PPI
MS.gene062975	MS.gene062974	PPI
MS.gene062975	MS.gene031201	PPI
MS.gene062975	MS.gene014670	PPI
MS.gene062975	MS.gene062943	PPI
MS.gene062975	MS.gene057801	PPI
MS.gene062975	MS.gene77524	PPI
MS.gene062975	MS.gene68162	PPI
MS.gene062975	MS.gene75675	PPI
MS.gene062975	MS.gene79887	PPI
MS.gene062975	MS.gene75679	PPI
MS.gene062975	MS.gene68761	PPI
MS.gene062975	MS.gene40325	PPI
MS.gene062975	MS.gene75677	PPI
MS.gene062975	MS.gene50677	PPI
MS.gene062975	MS.gene79890	PPI
MS.gene062975	MS.gene50684	PPI
MS.gene062975	MS.gene057797	PPI
MS.gene062975	MS.gene045362	PPI
MS.gene062975	MS.gene003614	PPI
MS.gene062975	MS.gene23372	PPI
MS.gene062975	MS.gene014668	PPI
MS.gene062975	MS.gene79886	PPI
MS.gene062975	MS.gene50765	PPI
MS.gene062975	MS.gene003596	PPI
MS.gene062975	MS.gene014667	PPI
MS.gene062975	MS.gene062988	PPI
MS.gene062975	MS.gene003624	PPI
MS.gene062975	MS.gene23371	PPI
MS.gene062975	MS.gene003583	PPI
MS.gene062975	MS.gene062951	PPI
MS.gene062975	MS.gene003608	PPI
MS.gene062975	MS.gene77527	PPI
MS.gene062975	MS.gene003591	PPI
MS.gene062975	MS.gene79891	PPI
MS.gene062975	MS.gene057795	PPI
MS.gene062975	MS.gene045367	PPI
MS.gene062975	MS.gene045364	PPI
MS.gene062975	MS.gene50665	PPI
MS.gene062975	MS.gene045363	PPI
MS.gene062975	MS.gene062973	PPI
MS.gene062975	MS.gene50652	PPI
MS.gene062975	MS.gene014669	PPI
MS.gene062975	MS.gene057808	PPI
MS.gene062975	MS.gene002380	PPI
MS.gene062975	MS.gene77529	PPI
MS.gene062975	MS.gene68165	PPI
MS.gene062975	MS.gene77526	PPI
MS.gene062975	MS.gene77523	PPI
MS.gene062975	MS.gene045366	PPI
MS.gene062975	MS.gene045365	PPI
MS.gene062975	MS.gene68163	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062975.t1	MTR_4g072480	94.805	77	4	0	1	77	1	77	9.09e-50	154
MS.gene062975.t1	MTR_4g072680	96.053	76	3	0	1	76	1	76	1.36e-49	153
MS.gene062975.t1	MTR_4g072460	93.421	76	5	0	1	76	1	76	2.75e-48	150
MS.gene062975.t1	MTR_4g072620	89.610	77	8	0	1	77	1	77	1.01e-47	149
MS.gene062975.t1	MTR_4g072730	85.714	77	11	0	1	77	1	77	2.01e-45	143
MS.gene062975.t1	MTR_4g072570	80.263	76	15	0	1	76	1	76	6.78e-40	129
MS.gene062975.t1	MTR_4g072660	80.519	77	10	1	1	77	1	72	1.34e-39	128
MS.gene062975.t1	MTR_4g072530	77.922	77	17	0	1	77	1	77	2.73e-38	124
MS.gene062975.t1	MTR_4g072500	77.922	77	17	0	1	77	1	77	2.80e-38	125
MS.gene062975.t1	MTR_3g084150	73.684	76	20	0	1	76	1	76	7.89e-37	121
MS.gene062975.t1	MTR_4g072640	72.727	77	21	0	1	77	1	77	4.12e-36	119
MS.gene062975.t1	MTR_3g084180	72.368	76	21	0	1	76	1	76	4.69e-36	119
MS.gene062975.t1	MTR_8g461410	72.368	76	21	0	1	76	1	76	4.85e-36	119
MS.gene062975.t1	MTR_3g110042	73.684	76	20	0	1	76	1	76	1.90e-35	117
MS.gene062975.t1	MTR_4g072310	70.130	77	22	1	1	76	1	77	1.20e-34	115
MS.gene062975.t1	MTR_4g072240	68.831	77	23	1	1	76	1	77	1.53e-34	115
MS.gene062975.t1	MTR_4g072870	68.831	77	23	1	1	76	1	77	1.99e-34	115
MS.gene062975.t1	MTR_4g072230	71.429	77	19	2	1	76	1	75	2.53e-34	114
MS.gene062975.t1	MTR_3g084250	67.949	78	25	0	1	78	1	78	9.14e-34	113
MS.gene062975.t1	MTR_4g072880	70.130	77	22	1	1	76	1	77	1.12e-33	113
MS.gene062975.t1	MTR_4g072220	66.234	77	25	1	1	76	1	77	2.87e-33	112
MS.gene062975.t1	MTR_4g072300	66.234	77	25	1	1	76	1	77	2.89e-33	112
MS.gene062975.t1	MTR_3g117630	73.239	71	19	0	6	76	6	76	3.63e-33	112
MS.gene062975.t1	MTR_4g072370	65.385	78	25	1	1	78	1	76	3.74e-33	112
MS.gene062975.t1	MTR_3g084240	67.105	76	25	0	1	76	1	76	4.46e-33	111
MS.gene062975.t1	MTR_4g072270	68.831	77	23	1	1	76	1	77	6.17e-33	111
MS.gene062975.t1	MTR_4g072490	66.667	78	21	1	1	78	1	73	9.98e-33	110
MS.gene062975.t1	MTR_4g072630	66.667	78	22	1	1	78	1	74	1.17e-32	110
MS.gene062975.t1	MTR_4g072580	66.234	77	21	1	1	77	1	72	1.22e-32	110
MS.gene062975.t1	MTR_3g084170	67.105	76	25	0	1	76	1	76	1.83e-32	110
MS.gene062975.t1	MTR_4g072260	64.935	77	26	1	1	76	1	77	2.53e-32	109
MS.gene062975.t1	MTR_4g072890	64.935	77	26	1	1	76	1	77	2.87e-32	109
MS.gene062975.t1	MTR_4g072550	89.474	57	6	0	20	76	31	87	2.93e-32	110
MS.gene062975.t1	MTR_5g008360	80.952	63	12	0	13	75	4	66	3.43e-32	109
MS.gene062975.t1	MTR_4g072860	67.949	78	24	1	1	77	1	78	3.47e-32	109
MS.gene062975.t1	MTR_4g072250	71.429	77	20	2	1	76	1	76	6.15e-32	108
MS.gene062975.t1	MTR_3g084220	67.105	76	25	0	1	76	1	76	6.22e-32	108
MS.gene062975.t1	MTR_3g084210	66.250	80	27	0	1	80	1	80	8.82e-32	108
MS.gene062975.t1	MTR_4g072740	66.667	78	21	1	1	78	1	73	9.39e-32	108
MS.gene062975.t1	MTR_8g461420	67.500	80	26	0	1	80	1	80	1.01e-31	108
MS.gene062975.t1	MTR_3g110038	64.474	76	27	0	1	76	1	76	1.90e-31	107
MS.gene062975.t1	MTR_4g072330	61.538	78	28	1	1	78	1	76	2.16e-31	107
MS.gene062975.t1	MTR_3g084190	67.500	80	26	0	1	80	1	80	2.33e-31	107
MS.gene062975.t1	MTR_4g072600	65.385	78	22	1	1	78	1	73	2.49e-31	107
MS.gene062975.t1	MTR_3g084230	67.500	80	26	0	1	80	1	80	2.52e-31	107
MS.gene062975.t1	MTR_4g072830	62.500	80	28	1	1	80	1	78	2.93e-31	107
MS.gene062975.t1	MTR_3g110032	63.291	79	28	1	1	78	1	79	4.10e-31	107
MS.gene062975.t1	MTR_4g072610	62.963	81	24	1	1	81	1	75	4.13e-31	106
MS.gene062975.t1	MTR_3g084160	67.500	80	26	0	1	80	1	80	1.02e-30	105
MS.gene062975.t1	MTR_4g072465	62.821	78	25	1	1	78	1	74	1.55e-30	105
MS.gene062975.t1	MTR_4g072470	65.789	76	21	1	1	76	1	71	3.14e-30	104
MS.gene062975.t1	MTR_3g084260	64.935	77	26	1	1	76	1	77	3.65e-30	104
MS.gene062975.t1	MTR_4g072540	65.000	80	24	1	1	80	1	76	1.61e-29	102
MS.gene062975.t1	MTR_4g072750	63.415	82	28	1	1	80	1	82	2.32e-29	102
MS.gene062975.t1	MTR_4g050700	64.935	77	26	1	1	76	1	77	2.49e-29	102
MS.gene062975.t1	MTR_2g012410	64.935	77	26	1	1	76	1	77	2.49e-29	102
MS.gene062975.t1	MTR_4g072820	67.532	77	18	2	1	76	1	71	3.61e-29	101
MS.gene062975.t1	MTR_5g008350	57.317	82	30	1	1	82	1	77	4.02e-29	101
MS.gene062975.t1	MTR_4g072720	65.000	80	24	1	1	80	1	76	7.26e-29	100
MS.gene062975.t1	MTR_3g110045	66.667	78	26	0	3	80	62	139	9.07e-29	102
MS.gene062975.t1	MTR_4g072850	65.823	79	25	1	1	77	1	79	1.18e-28	100
MS.gene062975.t1	MTR_3g084200	61.111	72	28	0	1	72	1	72	4.10e-28	99.8
MS.gene062975.t1	MTR_4g072380	55.102	98	33	3	1	97	1	88	8.40e-28	98.2
MS.gene062975.t1	MTR_4g072650	61.538	78	24	1	1	78	1	72	8.94e-28	98.2
MS.gene062975.t1	MTR_4g072590	65.385	78	22	1	1	78	1	73	1.36e-27	97.8
MS.gene062975.t1	MTR_4g072670	61.250	80	27	1	1	80	1	76	1.92e-27	97.4
MS.gene062975.t1	MTR_4g072510	62.500	80	26	1	1	80	1	76	4.45e-27	96.3
MS.gene062975.t1	MTR_5g008370	75.000	60	15	0	18	77	18	77	6.73e-27	97.4
MS.gene062975.t1	MTR_4g072940	77.193	57	13	0	21	77	53	109	8.43e-27	97.1
MS.gene062975.t1	MTR_4g072770	59.740	77	21	1	1	77	1	67	4.32e-25	91.3
MS.gene062975.t1	MTR_4g072980	55.696	79	29	2	1	78	8	81	3.84e-24	89.4
MS.gene062975.t1	MTR_4g072690	61.250	80	26	1	1	80	1	75	4.36e-24	89.0
MS.gene062975.t1	MTR_4g072280	54.667	75	33	1	1	74	1	75	7.32e-24	88.2
MS.gene062975.t1	MTR_3g117640	74.545	55	13	1	25	78	34	88	2.87e-23	87.4
MS.gene062975.t1	MTR_4g072560	54.054	74	33	1	8	80	15	88	6.51e-23	86.3
MS.gene062975.t1	MTR_4g072450	60.256	78	26	1	1	78	1	73	7.75e-23	85.5
MS.gene062975.t1	MTR_4g072910	54.054	74	33	1	9	81	18	91	1.19e-22	85.5
MS.gene062975.t1	MTR_4g072290	51.948	77	22	2	1	76	1	63	6.21e-20	78.2
MS.gene062975.t1	MTR_3g117610	60.714	56	21	1	23	78	43	97	6.05e-19	76.6
MS.gene062975.t1	MTR_6g077850	50.877	57	28	0	24	80	33	89	6.42e-19	76.3
MS.gene062975.t1	MTR_3g110035	60.000	55	22	0	26	80	72	126	1.32e-18	76.3
MS.gene062975.t1	MTR_3g113310	58.182	55	22	1	23	77	40	93	1.93e-18	75.1
MS.gene062975.t1	MTR_4g072190	55.000	60	26	1	19	78	30	88	5.68e-18	73.6
MS.gene062975.t1	MTR_4g072930	54.237	59	23	1	27	81	43	101	9.07e-17	70.9
MS.gene062975.t1	MTR_8g076040	57.407	54	22	1	24	77	40	92	1.11e-15	67.8
MS.gene062975.t1	MTR_5g021820	50.000	58	28	1	24	81	39	95	1.39e-15	67.8
MS.gene062975.t1	MTR_1g024085	38.318	107	58	3	24	129	36	135	6.00e-14	64.3
MS.gene062975.t1	MTR_4g124750	44.156	77	40	2	4	78	46	121	2.15e-13	63.2
MS.gene062975.t1	MTR_2g043960	54.545	55	24	1	24	78	75	128	2.67e-13	63.2
MS.gene062975.t1	MTR_1g009150	53.571	56	25	1	25	80	71	125	2.01e-12	61.2
MS.gene062975.t1	MTR_8g461210	40.260	77	45	1	4	80	14	89	5.13e-12	58.9
MS.gene062975.t1	MTR_0388s0020	44.776	67	33	2	15	78	65	130	1.73e-11	58.2
MS.gene062975.t1	MTR_7g118010	53.571	56	23	2	25	78	23	77	1.93e-11	57.0
MS.gene062975.t1	MTR_4g025830	50.877	57	24	2	25	78	79	134	2.16e-11	58.2
MS.gene062975.t1	MTR_3g092220	43.077	65	34	2	16	78	24	87	2.38e-11	57.0
MS.gene062975.t1	MTR_4g005310	41.026	78	41	3	21	97	58	131	2.78e-11	57.8
MS.gene062975.t1	MTR_4g005320	46.667	60	31	1	25	84	70	128	3.58e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062975.t1	AT2G21200	62.500	72	25	2	7	76	2	73	5.09e-26	94.0
MS.gene062975.t1	AT4G34800	50.505	99	40	3	1	96	1	93	7.82e-26	93.6
MS.gene062975.t1	AT4G38840	76.364	55	12	1	24	77	32	86	3.96e-25	92.0
MS.gene062975.t1	AT4G34770	56.098	82	34	1	4	83	14	95	7.75e-25	91.3
MS.gene062975.t1	AT4G38825	56.757	74	28	2	7	76	2	75	3.33e-24	89.4
MS.gene062975.t1	AT2G21210	53.086	81	33	2	1	76	1	81	4.32e-24	89.4
MS.gene062975.t1	AT2G21210	53.086	81	33	2	1	76	68	148	1.91e-23	89.7
MS.gene062975.t1	AT4G38850	57.692	78	29	2	7	80	2	79	2.01e-23	87.4
MS.gene062975.t1	AT5G18020	54.545	77	33	2	1	76	1	76	6.97e-23	85.9
MS.gene062975.t1	AT3G03840	59.211	76	26	2	3	76	8	80	1.13e-22	85.5
MS.gene062975.t1	AT5G18080	55.844	77	32	2	1	76	1	76	1.85e-22	84.7
MS.gene062975.t1	AT4G34810	64.407	59	17	1	24	78	47	105	1.88e-22	85.5
MS.gene062975.t1	AT3G03850	61.039	77	23	3	3	77	8	79	2.38e-22	84.7
MS.gene062975.t1	AT4G13790	48.193	83	41	1	1	81	1	83	2.40e-22	84.7
MS.gene062975.t1	AT5G18060	54.667	75	29	2	7	76	2	76	2.53e-22	84.3
MS.gene062975.t1	AT5G18050	53.333	75	30	2	7	76	2	76	1.20e-21	82.8
MS.gene062975.t1	AT5G18030	54.795	73	30	2	7	76	2	74	1.51e-21	82.4
MS.gene062975.t1	AT3G03820	61.111	72	22	2	8	76	13	81	1.77e-21	82.4
MS.gene062975.t1	AT3G03830	55.263	76	25	2	3	75	8	77	8.88e-21	80.5
MS.gene062975.t1	AT5G18010	51.948	77	35	2	1	76	1	76	2.75e-20	79.3
MS.gene062975.t1	AT4G34790	62.500	56	19	1	25	78	39	94	5.73e-19	76.6
MS.gene062975.t1	AT4G38860	58.929	56	23	0	23	78	38	93	1.57e-18	75.1
MS.gene062975.t1	AT1G75580	60.714	56	21	1	23	78	42	96	8.97e-18	73.6
MS.gene062975.t1	AT2G21220	58.929	56	22	1	23	78	38	92	9.02e-18	73.2
MS.gene062975.t1	AT4G34760	58.182	55	22	1	23	77	41	94	3.38e-17	72.0
MS.gene062975.t1	AT4G34780	60.345	58	20	1	23	77	26	83	3.56e-17	72.0
MS.gene062975.t1	AT1G19830	57.143	56	23	1	23	78	46	100	2.69e-16	69.7
MS.gene062975.t1	AT4G36110	55.556	54	23	1	24	77	39	91	1.14e-14	65.5
MS.gene062975.t1	AT2G16580	50.847	59	28	1	23	81	42	99	1.15e-14	65.5
MS.gene062975.t1	AT2G18010	51.724	58	27	1	24	81	47	103	2.13e-14	64.7
MS.gene062975.t1	AT3G43120	47.692	65	33	1	14	78	68	131	9.07e-14	64.3
MS.gene062975.t1	AT5G20810	54.545	55	24	1	24	78	78	131	1.71e-12	61.2
MS.gene062975.t1	AT5G20810	50.000	62	30	1	17	78	71	131	1.94e-12	61.6
MS.gene062975.t1	AT3G20220	45.070	71	33	3	11	80	37	102	2.01e-12	60.1
MS.gene062975.t1	AT1G75590	35.606	132	75	5	4	129	16	143	4.58e-12	59.7
MS.gene062975.t1	AT5G66260	49.123	57	28	1	25	81	34	89	1.04e-11	57.8
MS.gene062975.t1	AT3G51200	50.980	51	24	1	26	76	41	90	1.32e-11	57.4
MS.gene062975.t1	AT5G10990	37.037	108	55	4	24	129	40	136	2.60e-11	57.8
MS.gene062975.t1	AT3G09870	47.619	63	30	2	24	84	44	105	2.81e-11	57.0
MS.gene062975.t1	AT3G12830	43.836	73	39	2	4	76	30	100	5.57e-11	56.6

Find 27 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAACAAGTCTTGAAATGA+AGG	0.342172	4.2:+35443598	None:intergenic
AGTCAAGCCGAGGAAGAGTT+TGG	0.367159	4.2:-35443577	MS.gene062975:CDS
TTACATGGAATTAGCAAAAC+AGG	0.383422	4.2:+35443095	None:intergenic
CTCTTAGAATAGAGTTTAAA+CGG	0.400936	4.2:+35443740	None:intergenic
GGTTTGTTCAAGTATGATAC+AGG	0.410295	4.2:+35443619	None:intergenic
CGTTTAAACTCTATTCTAAG+AGG	0.413979	4.2:-35443739	MS.gene062975:CDS
CGTGTATGTTGGAGAGAAAC+TGG	0.417120	4.2:-35443656	MS.gene062975:CDS
AAAATCAGTAGAAGTGAGAA+AGG	0.471464	4.2:-35443689	MS.gene062975:CDS
AAAATCTATGGGCGTTCCAA+AGG	0.472091	4.2:+35441968	None:intergenic
AAATCTATGGGCGTTCCAAA+GGG	0.529875	4.2:+35441969	None:intergenic
TACACAGCTAGATATCCCTT+TGG	0.531737	4.2:-35441984	MS.gene062975:CDS
ATCATATCCAAACTCTTCCT+CGG	0.532636	4.2:+35443570	None:intergenic
GGCTATGTTGCCGTGTATGT+TGG	0.537170	4.2:-35443667	MS.gene062975:CDS
GACATCTTCCGTACAAGGAA+TGG	0.540589	4.2:+35443528	None:intergenic
AAACTCTATTCTAAGAGGAT+CGG	0.556160	4.2:-35443734	MS.gene062975:CDS
GTTTGGATATGATCATCCCA+TGG	0.570301	4.2:-35443560	MS.gene062975:CDS
AAATCAGTAGAAGTGAGAAA+GGG	0.578349	4.2:-35443688	MS.gene062975:CDS
AGGAATGGTGAGGCCACCCA+TGG	0.587754	4.2:+35443543	None:intergenic
CAGTTTCTCTCCAACATACA+CGG	0.594831	4.2:+35443657	None:intergenic
GGATATGATCATCCCATGGG+TGG	0.600402	4.2:-35443556	MS.gene062975:CDS
AGACTTGTTGAGTCAAGCCG+AGG	0.633253	4.2:-35443587	MS.gene062975:CDS
TTTGGATATGATCATCCCAT+GGG	0.641263	4.2:-35443559	MS.gene062975:CDS
GGAATGGTGAGGCCACCCAT+GGG	0.648249	4.2:+35443544	None:intergenic
CTTCCGTACAAGGAATGGTG+AGG	0.686019	4.2:+35443533	None:intergenic
GTTTGTTCAAGTATGATACA+GGG	0.688837	4.2:+35443620	None:intergenic
GGATATCTAGCTGTGTACGT+AGG	0.696914	4.2:+35441990	None:intergenic
ATGTTGGAGAGAAACTGGCG+CGG	0.774668	4.2:-35443651	MS.gene062975:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTCATATTTATAAATAAG+GGG	-	chr4.2:35443367-35443386	MS.gene062975:intron	10.0%
!!	ATAATTTAGCCAAATTAATT+AGG	-	chr4.2:35442685-35442704	MS.gene062975:intron	15.0%
!!!	AAGAATTTTAATTACTTACA+TGG	+	chr4.2:35442644-35442663	None:intergenic	15.0%
!!!	AGTTTTTCAAATTATTTTGA+AGG	+	chr4.2:35442939-35442958	None:intergenic	15.0%
!!!	TTTGCTTTAAATATGATATA+GGG	-	chr4.2:35443684-35443703	MS.gene062975:CDS	15.0%
!!!	TTTTGCTTTAAATATGATAT+AGG	-	chr4.2:35443683-35443702	MS.gene062975:CDS	15.0%
!!	ATATTTCACATTTATTCAGA+AGG	-	chr4.2:35442761-35442780	MS.gene062975:intron	20.0%
!!	TATCTCTAATACTAGATAAT+TGG	-	chr4.2:35443092-35443111	MS.gene062975:intron	20.0%
!!	TATTTCACATTTATTCAGAA+GGG	-	chr4.2:35442762-35442781	MS.gene062975:intron	20.0%
!!!	AACTAATTTGCTACAAAAAT+TGG	-	chr4.2:35443496-35443515	MS.gene062975:intron	20.0%
!!!	GTTGTTTTGTAAAAAAACAA+TGG	+	chr4.2:35443159-35443178	None:intergenic	20.0%
!!!	TTTCTATTTTTTTTGCAACA+GGG	-	chr4.2:35443265-35443284	MS.gene062975:intron	20.0%
!!!	TTTGAGACAATTTTCATTAT+AGG	-	chr4.2:35442288-35442307	MS.gene062975:intron	20.0%
!!!	TTTTTTTACAAAACAACAAC+AGG	-	chr4.2:35443162-35443181	MS.gene062975:intron	20.0%
!	AAACTAGATGTTATGAAACA+TGG	-	chr4.2:35442954-35442973	MS.gene062975:intron	25.0%
!	AAAGAACTAACTATTGTAGT+AGG	+	chr4.2:35442533-35442552	None:intergenic	25.0%
!	AAGTAGTATTAGTACTATAG+TGG	+	chr4.2:35443060-35443079	None:intergenic	25.0%
!	AATGGAGAAAATTTACTATC+TGG	-	chr4.2:35442349-35442368	MS.gene062975:intron	25.0%
!	ACGAATTTACTTCTAATGTT+TGG	-	chr4.2:35442891-35442910	MS.gene062975:intron	25.0%
!	CATATAATTTCTTCCTTGAA+TGG	-	chr4.2:35442222-35442241	MS.gene062975:intron	25.0%
!	CTCTTAGAATAGAGTTTAAA+CGG	+	chr4.2:35441984-35442003	None:intergenic	25.0%
!	GTCATTAAACAAGAAAAAAG+AGG	+	chr4.2:35442399-35442418	None:intergenic	25.0%
!	GTTATTTATTCTTCATAAGC+AGG	+	chr4.2:35442488-35442507	None:intergenic	25.0%
!	TATTTAACCAAACTAACCAA+TGG	+	chr4.2:35442744-35442763	None:intergenic	25.0%
!	TCAAGGAAGAAATTATATGT+TGG	+	chr4.2:35442221-35442240	None:intergenic	25.0%
!	TTAGCCAAATTAATTAGGTT+CGG	-	chr4.2:35442690-35442709	MS.gene062975:intron	25.0%
!	TTGAATCAACTTCAAACTAT+AGG	+	chr4.2:35442799-35442818	None:intergenic	25.0%
!!	CAAAAGAGAAAATCTTTTGA+AGG	-	chr4.2:35443468-35443487	MS.gene062975:intron	25.0%
!!	GTAAATATTACCCTTTTCAT+GGG	-	chr4.2:35442325-35442344	MS.gene062975:intron	25.0%
!!	TGTAAATATTACCCTTTTCA+TGG	-	chr4.2:35442324-35442343	MS.gene062975:intron	25.0%
!!!	GTTTCTATTTTTTTTGCAAC+AGG	-	chr4.2:35443264-35443283	MS.gene062975:intron	25.0%
!!!	TCTTTTGTTGTTATCATACT+TGG	+	chr4.2:35443455-35443474	None:intergenic	25.0%
!!!	TTCTATTTTTTTTGCAACAG+GGG	-	chr4.2:35443266-35443285	MS.gene062975:intron	25.0%
	AAAATCAGTAGAAGTGAGAA+AGG	-	chr4.2:35442032-35442051	MS.gene062975:intron	30.0%
	AAATCAGTAGAAGTGAGAAA+GGG	-	chr4.2:35442033-35442052	MS.gene062975:intron	30.0%
	ATTTGAGTATGATCATCCAA+TGG	+	chr4.2:35443627-35443646	None:intergenic	30.0%
	CAAATTTCACTAGCTATAGT+TGG	-	chr4.2:35442447-35442466	MS.gene062975:intron	30.0%
	CGTTTAAACTCTATTCTAAG+AGG	-	chr4.2:35441982-35442001	MS.gene062975:CDS	30.0%
	GTTTGTTCAAGTATGATACA+GGG	+	chr4.2:35442104-35442123	None:intergenic	30.0%
	TAAGATTCATAGTCCATTCA+AGG	+	chr4.2:35442238-35442257	None:intergenic	30.0%
	TTACATGGAATTAGCAAAAC+AGG	+	chr4.2:35442629-35442648	None:intergenic	30.0%
	TTTACTTCTAATGTTTGGCA+TGG	-	chr4.2:35442896-35442915	MS.gene062975:intron	30.0%
	TTTGAGTATGATCATCCAAT+GGG	+	chr4.2:35443626-35443645	None:intergenic	30.0%
!	AAACTCTATTCTAAGAGGAT+CGG	-	chr4.2:35441987-35442006	MS.gene062975:CDS	30.0%
!!!	TCTATTTTTTTTGCAACAGG+GGG	-	chr4.2:35443267-35443286	MS.gene062975:intron	30.0%
	AAACTAACCAATGGAAGCAA+AGG	+	chr4.2:35442735-35442754	None:intergenic	35.0%
	AAGAAAACTTCTTCTCTGCA+AGG	-	chr4.2:35443587-35443606	MS.gene062975:CDS	35.0%
	AATTGTAGGTGGCAAGTAAA+TGG	-	chr4.2:35443126-35443145	MS.gene062975:intron	35.0%
	ACTCAAATTGTTCCTCACTT+TGG	-	chr4.2:35443639-35443658	MS.gene062975:CDS	35.0%
	ATCATATCCAAACTCTTCCT+CGG	+	chr4.2:35442154-35442173	None:intergenic	35.0%
	CTCAACAAGTCTTGAAATGA+AGG	+	chr4.2:35442126-35442145	None:intergenic	35.0%
	TAGGCCGAACCTAATTAATT+TGG	+	chr4.2:35442697-35442716	None:intergenic	35.0%
	TTCTCCATTTCCCATGAAAA+GGG	+	chr4.2:35442338-35442357	None:intergenic	35.0%
	TTTCTCCATTTCCCATGAAA+AGG	+	chr4.2:35442339-35442358	None:intergenic	35.0%
	TTTGGATATGATCATCCCAT+GGG	-	chr4.2:35442162-35442181	MS.gene062975:intron	35.0%
!	ATTACCCTTTTCATGGGAAA+TGG	-	chr4.2:35442331-35442350	MS.gene062975:intron	35.0%
!	GGTTTGTTCAAGTATGATAC+AGG	+	chr4.2:35442105-35442124	None:intergenic	35.0%
!	TTTGCTTCCATTGGTTAGTT+TGG	-	chr4.2:35442734-35442753	MS.gene062975:intron	35.0%
!!!	CAAAACAGGTTTTGTTGCAT+AGG	+	chr4.2:35442615-35442634	None:intergenic	35.0%
!!!	CTATTTTTTTTGCAACAGGG+GGG	-	chr4.2:35443268-35443287	MS.gene062975:intron	35.0%
!!!	TATTTTTTTTGCAACAGGGG+GGG	-	chr4.2:35443269-35443288	MS.gene062975:intron	35.0%
	AAAGAGCCATAAGATACAGC+AGG	+	chr4.2:35442834-35442853	None:intergenic	40.0%
	AAGAGCCATAAGATACAGCA+GGG	+	chr4.2:35442833-35442852	None:intergenic	40.0%
	AGTCTATGTGAGTATCCTAC+AGG	+	chr4.2:35442262-35442281	None:intergenic	40.0%
	CAGTTTCTCTCCAACATACA+CGG	+	chr4.2:35442067-35442086	None:intergenic	40.0%
	GACTATGAATCTTACCCTGT+AGG	-	chr4.2:35442244-35442263	MS.gene062975:intron	40.0%
	GTCTATGTGAGTATCCTACA+GGG	+	chr4.2:35442261-35442280	None:intergenic	40.0%
	GTTATGAAACATGGCATCCA+TGG	-	chr4.2:35442963-35442982	MS.gene062975:intron	40.0%
	GTTGCATAGGAAGATTTAGC+TGG	+	chr4.2:35442602-35442621	None:intergenic	40.0%
	GTTTGGATATGATCATCCCA+TGG	-	chr4.2:35442161-35442180	MS.gene062975:intron	40.0%
	TAATGTTTGGCATGGAGTTC+TGG	-	chr4.2:35442904-35442923	MS.gene062975:intron	40.0%
	TACACAGCTAGATATCCCTT+TGG	-	chr4.2:35443737-35443756	MS.gene062975:CDS	40.0%
!	AAGGCAGTTTCTGAAGACAT+AGG	+	chr4.2:35442716-35442735	None:intergenic	40.0%
!	CAACTGTCTGAAATTGTAGG+TGG	-	chr4.2:35443115-35443134	MS.gene062975:CDS	40.0%
!	TGGCAACTGTCTGAAATTGT+AGG	-	chr4.2:35443112-35443131	MS.gene062975:CDS	40.0%
	AACTGCCCTGCTGTATCTTA+TGG	-	chr4.2:35442825-35442844	MS.gene062975:intron	45.0%
	AGAATTGCTGAGCCAAAGTG+AGG	+	chr4.2:35443654-35443673	None:intergenic	45.0%
	ATGGCATCCATGGAAGGAAT+GGG	-	chr4.2:35442973-35442992	MS.gene062975:intron	45.0%
	CGTGTATGTTGGAGAGAAAC+TGG	-	chr4.2:35442065-35442084	MS.gene062975:intron	45.0%
	GAAACTGCCTTTGCTTCCAT+TGG	-	chr4.2:35442725-35442744	MS.gene062975:intron	45.0%
	GACATCTTCCGTACAAGGAA+TGG	+	chr4.2:35442196-35442215	None:intergenic	45.0%
	GAGTATGATCATCCAATGGG+TGG	+	chr4.2:35443623-35443642	None:intergenic	45.0%
	GGATATCTAGCTGTGTACGT+AGG	+	chr4.2:35443734-35443753	None:intergenic	45.0%
	TGAAACATGGCATCCATGGA+AGG	-	chr4.2:35442967-35442986	MS.gene062975:intron	45.0%
	TGGAAGACATCTTCCGTACA+AGG	+	chr4.2:35442201-35442220	None:intergenic	45.0%
!	CACTTTGGCTCAGCAATTCT+TGG	-	chr4.2:35443654-35443673	MS.gene062975:CDS	45.0%
!	GTTTTCTCCCATTCCTTCCA+TGG	+	chr4.2:35442983-35443002	None:intergenic	45.0%
!!!	ATTTTCATATTTATAAATAA+GGG	-	chr4.2:35443366-35443385	MS.gene062975:intron	5.0%
!!!	TATTTTCATATTTATAAATA+AGG	-	chr4.2:35443365-35443384	MS.gene062975:intron	5.0%
	AGTCAAGCCGAGGAAGAGTT+TGG	-	chr4.2:35442144-35442163	MS.gene062975:intron	50.0%
	ATGTTGGAGAGAAACTGGCG+CGG	-	chr4.2:35442070-35442089	MS.gene062975:intron	50.0%
	CATGGCATCCATGGAAGGAA+TGG	-	chr4.2:35442972-35442991	MS.gene062975:intron	50.0%
	CTTCCGTACAAGGAATGGTG+AGG	+	chr4.2:35442191-35442210	None:intergenic	50.0%
	GGAATAGTGAGACCACCCAT+TGG	-	chr4.2:35443608-35443627	MS.gene062975:CDS	50.0%
	GGATATGATCATCCCATGGG+TGG	-	chr4.2:35442165-35442184	MS.gene062975:intron	50.0%
	GGCTATGTTGCCGTGTATGT+TGG	-	chr4.2:35442054-35442073	MS.gene062975:intron	50.0%
	TGGCCTCACCATTCCTTGTA+CGG	-	chr4.2:35442185-35442204	MS.gene062975:intron	50.0%
!!	AGACTTGTTGAGTCAAGCCG+AGG	-	chr4.2:35442134-35442153	MS.gene062975:intron	50.0%
	AGGAATGGTGAGGCCACCCA+TGG	+	chr4.2:35442181-35442200	None:intergenic	60.0%
	GGAATGGTGAGGCCACCCAT+GGG	+	chr4.2:35442180-35442199	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35441973	35443770	35441973	ID=MS.gene062975
chr4.2	mRNA	35441973	35443770	35441973	ID=MS.gene062975.t1;Parent=MS.gene062975
chr4.2	exon	35443542	35443770	35443542	ID=MS.gene062975.t1.exon1;Parent=MS.gene062975.t1
chr4.2	CDS	35443542	35443770	35443542	ID=cds.MS.gene062975.t1;Parent=MS.gene062975.t1
chr4.2	exon	35443099	35443140	35443099	ID=MS.gene062975.t1.exon2;Parent=MS.gene062975.t1
chr4.2	CDS	35443099	35443140	35443099	ID=cds.MS.gene062975.t1;Parent=MS.gene062975.t1
chr4.2	exon	35442254	35442306	35442254	ID=MS.gene062975.t1.exon3;Parent=MS.gene062975.t1
chr4.2	CDS	35442254	35442306	35442254	ID=cds.MS.gene062975.t1;Parent=MS.gene062975.t1
chr4.2	exon	35441973	35442038	35441973	ID=MS.gene062975.t1.exon4;Parent=MS.gene062975.t1
chr4.2	CDS	35441973	35442038	35441973	ID=cds.MS.gene062975.t1;Parent=MS.gene062975.t1

Gene Sequence

>MS.gene062975

ATGGGTTTCCGTTTAAACTCTATTCTAAGAGGATCGGTAACAGCTAGACAAGCAACATCAAAATCAGTAGAAGTGAGAAAGGGCTATGTTGCCGTGTATGTTGGAGAGAAACTGGCGCGGTTTGTAGTCCCTGTATCATACTTGAACAAACCTTCATTTCAAGACTTGTTGAGTCAAGCCGAGGAAGAGTTTGGATATGATCATCCCATGGGTGGCCTCACCATTCCTTGTACGGAAGATGTCTTCCAACATATAATTTCTTCCTTGAATGGACTATGAATCTTACCCTGTAGGATACTCACATAGACTAGATTATTTGAGACAATTTTCATTATAGGCATCTTTTTTAATTGTAAATATTACCCTTTTCATGGGAAATGGAGAAAATTTACTATCTGGATAACAAATTCAAAAAATTACTGTCCTCTTTTTTCTTGTTTAATGACACATTGAGCTCATTTACTTTTTCAGTTACAAATTTCACTAGCTATAGTTGGTTCTTTAAATTTAAGCCTGCTTATGAAGAATAAATAACTTTGCTGATTTAAAAGATAAAGCCTACTACAATAGTTAGTTCTTTAAGTCTGTTGAAATAAGATAATCTGTTTCTTTTACTTCTGTAAAATCCAGCTAAATCTTCCTATGCAACAAAACCTGTTTTGCTAATTCCATGTAAGTAATTAAAATTCTTAAACAAAATCAATAACTATTGATAATTTAGCCAAATTAATTAGGTTCGGCCTATGTCTTCAGAAACTGCCTTTGCTTCCATTGGTTAGTTTGGTTAAATATTTCACATTTATTCAGAAGGGCTCTTTCAACTCCTATAGTTTGAAGTTGATTCAAATTTCAAACTGCCCTGCTGTATCTTATGGCTCTTTAGTCTTTAACTATTTAATTTTCTCAAAACATAAAATCACGAATTTACTTCTAATGTTTGGCATGGAGTTCTGGTAATTTTCTCCTTCAAAATAATTTGAAAAACTAGATGTTATGAAACATGGCATCCATGGAAGGAATGGGAGAAAACTCAAATAATCTTTGTATTGATGATAAAATGAATATTACAAATGAGTGAATTATTCCACTATAGTACTAATACTACTTGTAACTAAACTATATCTCTAATACTAGATAATTGGCAACTGTCTGAAATTGTAGGTGGCAAGTAAATGGAGATAAACCATTGTTTTTTTACAAAACAACAACAGGATCTATGACTAAAAACACAAAGAAAACTACAAATAAATCAATATAGTTAGATTCAGTGTTTTTCTTGAAGTTGTTTTAAGTTTCTATTTTTTTTGCAACAGGGGGGGATTCGATTTTATATTTGAAGTGAATGAATTTTTCATTTTATCAGAGAGATTATAAACTGAAAATTTAGCAATCTATTTTCATATTTATAAATAAGGGGAAGTAATAGAGAAATAATGTAACAGTGTTTAAAAATTTCTCATTCAGATGAATGTTTTACATCCAAGTATGATAACAACAAAAGAGAAAATCTTTTGAAGGTAAAAAACTAATTTGCTACAAAAATTGGTCAAATATCTATGTCAATCTTCTACTATCTTCAGAGAGCTACTTCAATTTAAGCTAGAAGTGATGTCTAAGAAAACTTCTTCTCTGCAAGGAATAGTGAGACCACCCATTGGATGATCATACTCAAATTGTTCCTCACTTTGGCTCAGCAATTCTTGGAATGATTTTTGCTTTAAATATGATATAGGGATCACAAATCTCTTCATTTTCTCTCCTACGTACACAGCTAGATATCCCTTTGGAACGCCCATAG

Protein sequence

>MS.gene062975.t1

MGFRLNSILRGSVTARQATSKSVEVRKGYVAVYVGEKLARFVVPVSYLNKPSFQDLLSQAEEEFGYDHPMGGLTIPSKSSYATKPVLLIPYECFTSKYDNNKRENLLKGSQISSFSLLRTQLDIPLERP