Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073317.t1 | RHN64690.1 | 98.2 | 55 | 1 | 0 | 1 | 55 | 324 | 378 | 6.00E-20 | 105.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073317.t1 | Q9LU68 | 80.0 | 55 | 7 | 1 | 1 | 55 | 369 | 419 | 1.5e-14 | 79.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073317.t1 | G7JUA6 | 98.2 | 55 | 1 | 0 | 1 | 55 | 324 | 378 | 4.3e-20 | 105.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene073317.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene073317 | 0.816063 | 6.71E-52 | -1.69E-46 |
| MS.gene049151 | MS.gene073317 | 0.81075 | 9.87E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene073317 | 0.818147 | 2.28E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene073317 | 0.844678 | 6.52E-59 | -1.69E-46 |
| MS.gene049284 | MS.gene073317 | 0.810311 | 1.23E-50 | -1.69E-46 |
| MS.gene049297 | MS.gene073317 | 0.848796 | 4.88E-60 | -1.69E-46 |
| MS.gene049346 | MS.gene073317 | 0.816229 | 6.16E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene073317 | 0.816149 | 6.42E-52 | -1.69E-46 |
| MS.gene049389 | MS.gene073317 | 0.817273 | 3.59E-52 | -1.69E-46 |
| MS.gene049491 | MS.gene073317 | 0.81874 | 1.67E-52 | -1.69E-46 |
| MS.gene049599 | MS.gene073317 | -0.802887 | 4.53E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene073317 | 0.884484 | 1.84E-71 | -1.69E-46 |
| MS.gene049910 | MS.gene073317 | 0.835546 | 1.58E-56 | -1.69E-46 |
| MS.gene049915 | MS.gene073317 | 0.815426 | 9.30E-52 | -1.69E-46 |
| MS.gene050099 | MS.gene073317 | 0.848993 | 4.30E-60 | -1.69E-46 |
| MS.gene050128 | MS.gene073317 | 0.846952 | 1.57E-59 | -1.69E-46 |
| MS.gene050168 | MS.gene073317 | 0.803103 | 4.09E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene073317 | 0.820582 | 6.36E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene073317 | 0.829922 | 3.92E-55 | -1.69E-46 |
| MS.gene050228 | MS.gene073317 | 0.821129 | 4.76E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene073317 | 0.889039 | 3.46E-73 | -1.69E-46 |
| MS.gene050330 | MS.gene073317 | -0.828725 | 7.66E-55 | -1.69E-46 |
| MS.gene050397 | MS.gene073317 | 0.844257 | 8.46E-59 | -1.69E-46 |
| MS.gene050404 | MS.gene073317 | 0.824145 | 9.46E-54 | -1.69E-46 |
| MS.gene050406 | MS.gene073317 | 0.835071 | 2.08E-56 | -1.69E-46 |
| MS.gene050426 | MS.gene073317 | -0.811616 | 6.40E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene073317 | 0.855383 | 6.55E-62 | -1.69E-46 |
| MS.gene050529 | MS.gene073317 | 0.866488 | 2.75E-65 | -1.69E-46 |
| MS.gene050558 | MS.gene073317 | 0.836414 | 9.49E-57 | -1.69E-46 |
| MS.gene050623 | MS.gene073317 | -0.841319 | 5.11E-58 | -1.69E-46 |
| MS.gene050642 | MS.gene073317 | 0.900794 | 5.13E-78 | -1.69E-46 |
| MS.gene050812 | MS.gene073317 | 0.803195 | 3.91E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene073317 | 0.828048 | 1.12E-54 | -1.69E-46 |
| MS.gene051171 | MS.gene073317 | 0.810525 | 1.10E-50 | -1.69E-46 |
| MS.gene051240 | MS.gene073317 | 0.843882 | 1.07E-58 | -1.69E-46 |
| MS.gene051242 | MS.gene073317 | 0.818096 | 2.34E-52 | -1.69E-46 |
| MS.gene051257 | MS.gene073317 | 0.801968 | 7.01E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene073317 | 0.83991 | 1.19E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene073317 | 0.813036 | 3.14E-51 | -1.69E-46 |
| MS.gene051662 | MS.gene073317 | 0.844183 | 8.85E-59 | -1.69E-46 |
| MS.gene051663 | MS.gene073317 | 0.852277 | 5.13E-61 | -1.69E-46 |
| MS.gene051860 | MS.gene073317 | 0.877442 | 6.27E-69 | -1.69E-46 |
| MS.gene051983 | MS.gene073317 | 0.877355 | 6.72E-69 | -1.69E-46 |
| MS.gene052011 | MS.gene073317 | -0.82586 | 3.72E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene073317 | 0.850643 | 1.49E-60 | -1.69E-46 |
| MS.gene052078 | MS.gene073317 | 0.860698 | 1.72E-63 | -1.69E-46 |
| MS.gene052176 | MS.gene073317 | 0.822769 | 1.98E-53 | -1.69E-46 |
| MS.gene052178 | MS.gene073317 | 0.866327 | 3.09E-65 | -1.69E-46 |
| MS.gene052188 | MS.gene073317 | 0.866341 | 3.06E-65 | -1.69E-46 |
| MS.gene052253 | MS.gene073317 | 0.843057 | 1.77E-58 | -1.69E-46 |
| MS.gene052309 | MS.gene073317 | -0.828835 | 7.21E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene073317 | 0.839047 | 2.00E-57 | -1.69E-46 |
| MS.gene052326 | MS.gene073317 | 0.805582 | 1.25E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene073317 | 0.867581 | 1.23E-65 | -1.69E-46 |
| MS.gene052600 | MS.gene073317 | 0.858595 | 7.40E-63 | -1.69E-46 |
| MS.gene05316 | MS.gene073317 | 0.800665 | 1.30E-48 | -1.69E-46 |
| MS.gene053209 | MS.gene073317 | 0.819142 | 1.36E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene073317 | 0.881166 | 3.01E-70 | -1.69E-46 |
| MS.gene053836 | MS.gene073317 | 0.905903 | 2.62E-80 | -1.69E-46 |
| MS.gene053907 | MS.gene073317 | 0.844637 | 6.68E-59 | -1.69E-46 |
| MS.gene053928 | MS.gene073317 | 0.828251 | 9.97E-55 | -1.69E-46 |
| MS.gene053971 | MS.gene073317 | 0.827369 | 1.63E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene073317 | 0.808415 | 3.13E-50 | -1.69E-46 |
| MS.gene054012 | MS.gene073317 | 0.806226 | 9.12E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene073317 | 0.815538 | 8.78E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene073317 | 0.803391 | 3.56E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene073317 | 0.840669 | 7.57E-58 | -1.69E-46 |
| MS.gene054473 | MS.gene073317 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene054667 | MS.gene073317 | 0.804116 | 2.52E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene073317 | 0.841228 | 5.40E-58 | -1.69E-46 |
| MS.gene054942 | MS.gene073317 | 0.848348 | 6.49E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene073317 | 0.844586 | 6.90E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene073317 | 0.805571 | 1.25E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene073317 | 0.864977 | 8.25E-65 | -1.69E-46 |
| MS.gene055390 | MS.gene073317 | 0.827541 | 1.48E-54 | -1.69E-46 |
| MS.gene055422 | MS.gene073317 | 0.884411 | 1.96E-71 | -1.69E-46 |
| MS.gene055428 | MS.gene073317 | 0.869672 | 2.60E-66 | -1.69E-46 |
| MS.gene05553 | MS.gene073317 | 0.853486 | 2.31E-61 | -1.69E-46 |
| MS.gene055604 | MS.gene073317 | 0.845883 | 3.08E-59 | -1.69E-46 |
| MS.gene055712 | MS.gene073317 | 0.821093 | 4.85E-53 | -1.69E-46 |
| MS.gene056314 | MS.gene073317 | 0.80501 | 1.64E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene073317 | 0.80487 | 1.76E-49 | -1.69E-46 |
| MS.gene056451 | MS.gene073317 | 0.805026 | 1.63E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene073317 | 0.865896 | 4.24E-65 | -1.69E-46 |
| MS.gene056659 | MS.gene073317 | 0.827925 | 1.20E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene073317 | 0.818905 | 1.54E-52 | -1.69E-46 |
| MS.gene056772 | MS.gene073317 | 0.816296 | 5.95E-52 | -1.69E-46 |
| MS.gene056796 | MS.gene073317 | 0.833569 | 4.95E-56 | -1.69E-46 |
| MS.gene056899 | MS.gene073317 | -0.803918 | 2.77E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene073317 | 0.873908 | 1.02E-67 | -1.69E-46 |
| MS.gene05702 | MS.gene073317 | 0.830253 | 3.26E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene073317 | 0.880302 | 6.14E-70 | -1.69E-46 |
| MS.gene057245 | MS.gene073317 | 0.830599 | 2.68E-55 | -1.69E-46 |
| MS.gene057260 | MS.gene073317 | 0.807263 | 5.51E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene073317 | 0.811108 | 8.26E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene073317 | 0.853349 | 2.53E-61 | -1.69E-46 |
| MS.gene057448 | MS.gene073317 | 0.808089 | 3.68E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene073317 | 0.80897 | 2.38E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene073317 | 0.902333 | 1.08E-78 | -1.69E-46 |
| MS.gene058082 | MS.gene073317 | 0.813465 | 2.52E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene073317 | 0.902783 | 6.81E-79 | -1.69E-46 |
| MS.gene058142 | MS.gene073317 | 0.898311 | 6.01E-77 | -1.69E-46 |
| MS.gene058201 | MS.gene073317 | 0.801043 | 1.08E-48 | -1.69E-46 |
| MS.gene058233 | MS.gene073317 | 0.824513 | 7.75E-54 | -1.69E-46 |
| MS.gene058276 | MS.gene073317 | 0.888018 | 8.56E-73 | -1.69E-46 |
| MS.gene058293 | MS.gene073317 | 0.803041 | 4.21E-49 | -1.69E-46 |
| MS.gene058365 | MS.gene073317 | 0.815247 | 1.02E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene073317 | 0.833507 | 5.13E-56 | -1.69E-46 |
| MS.gene058710 | MS.gene073317 | 0.834298 | 3.25E-56 | -1.69E-46 |
| MS.gene058749 | MS.gene073317 | 0.860058 | 2.69E-63 | -1.69E-46 |
| MS.gene058833 | MS.gene073317 | 0.86525 | 6.77E-65 | -1.69E-46 |
| MS.gene058845 | MS.gene073317 | 0.842646 | 2.28E-58 | -1.69E-46 |
| MS.gene05886 | MS.gene073317 | 0.804637 | 1.96E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene073317 | 0.803254 | 3.81E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene073317 | 0.877743 | 4.92E-69 | -1.69E-46 |
| MS.gene059247 | MS.gene073317 | 0.830372 | 3.05E-55 | -1.69E-46 |
| MS.gene059285 | MS.gene073317 | 0.829914 | 3.94E-55 | -1.69E-46 |
| MS.gene059294 | MS.gene073317 | 0.829206 | 5.86E-55 | -1.69E-46 |
| MS.gene059342 | MS.gene073317 | 0.820337 | 7.24E-53 | -1.69E-46 |
| MS.gene059518 | MS.gene073317 | 0.877094 | 8.27E-69 | -1.69E-46 |
| MS.gene059597 | MS.gene073317 | 0.860566 | 1.89E-63 | -1.69E-46 |
| MS.gene059676 | MS.gene073317 | 0.823535 | 1.31E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene073317 | 0.819312 | 1.24E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene073317 | 0.842448 | 2.57E-58 | -1.69E-46 |
| MS.gene059695 | MS.gene073317 | 0.836533 | 8.85E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene073317 | 0.928977 | 1.34E-92 | -1.69E-46 |
| MS.gene059894 | MS.gene073317 | 0.900335 | 8.12E-78 | -1.69E-46 |
| MS.gene059899 | MS.gene073317 | 0.863283 | 2.78E-64 | -1.69E-46 |
| MS.gene06037 | MS.gene073317 | 0.816015 | 6.87E-52 | -1.69E-46 |
| MS.gene060407 | MS.gene073317 | 0.854814 | 9.58E-62 | -1.69E-46 |
| MS.gene060476 | MS.gene073317 | 0.83947 | 1.55E-57 | -1.69E-46 |
| MS.gene06090 | MS.gene073317 | 0.807938 | 3.96E-50 | -1.69E-46 |
| MS.gene060994 | MS.gene073317 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene061000 | MS.gene073317 | 0.803621 | 3.20E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene073317 | 0.87917 | 1.55E-69 | -1.69E-46 |
| MS.gene061112 | MS.gene073317 | 0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene061159 | MS.gene073317 | 0.80384 | 2.88E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene073317 | 0.805122 | 1.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073317.t1 | MTR_4g127420 | 98.182 | 55 | 1 | 0 | 1 | 55 | 324 | 378 | 2.65e-31 | 111 |
| MS.gene073317.t1 | MTR_5g072780 | 83.784 | 37 | 6 | 0 | 19 | 55 | 174 | 210 | 1.05e-14 | 64.7 |
| MS.gene073317.t1 | MTR_8g104190 | 73.810 | 42 | 11 | 0 | 14 | 55 | 353 | 394 | 1.17e-14 | 65.9 |
| MS.gene073317.t1 | MTR_1g110870 | 76.923 | 39 | 9 | 0 | 17 | 55 | 380 | 418 | 1.63e-14 | 65.5 |
| MS.gene073317.t1 | MTR_7g032240 | 71.795 | 39 | 11 | 0 | 17 | 55 | 307 | 345 | 4.54e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073317.t1 | AT5G57180 | 80.000 | 55 | 7 | 1 | 1 | 55 | 369 | 419 | 1.09e-21 | 85.9 |
| MS.gene073317.t1 | AT5G57180 | 80.000 | 55 | 7 | 1 | 1 | 55 | 369 | 419 | 1.34e-21 | 85.5 |
| MS.gene073317.t1 | AT4G25990 | 75.926 | 54 | 9 | 1 | 2 | 55 | 328 | 377 | 2.33e-19 | 79.0 |
| MS.gene073317.t1 | AT4G25990 | 75.926 | 54 | 9 | 1 | 2 | 55 | 328 | 377 | 2.71e-19 | 79.0 |
| MS.gene073317.t1 | AT4G25990 | 75.926 | 54 | 9 | 1 | 2 | 55 | 328 | 377 | 3.28e-19 | 79.0 |
| MS.gene073317.t1 | AT1G73870 | 73.333 | 45 | 12 | 0 | 11 | 55 | 337 | 381 | 7.94e-16 | 69.3 |
| MS.gene073317.t1 | AT1G07050 | 83.784 | 37 | 6 | 0 | 19 | 55 | 151 | 187 | 4.75e-15 | 65.5 |
| MS.gene073317.t1 | AT1G25440 | 70.213 | 47 | 14 | 0 | 9 | 55 | 351 | 397 | 5.15e-15 | 67.0 |
| MS.gene073317.t1 | AT1G68520 | 73.810 | 42 | 11 | 0 | 14 | 55 | 352 | 393 | 1.53e-14 | 65.9 |
| MS.gene073317.t1 | AT1G07050 | 83.784 | 37 | 6 | 0 | 19 | 55 | 151 | 187 | 1.40e-13 | 61.6 |
| MS.gene073317.t1 | AT5G14370 | 74.359 | 39 | 10 | 0 | 17 | 55 | 292 | 330 | 4.28e-13 | 61.6 |
| MS.gene073317.t1 | AT1G49130 | 72.973 | 37 | 10 | 0 | 19 | 55 | 271 | 307 | 2.35e-11 | 56.6 |
| MS.gene073317.t1 | AT1G49130 | 72.973 | 37 | 10 | 0 | 19 | 55 | 278 | 314 | 3.68e-11 | 56.2 |
Find 12 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATCATACCTTCATTCT+TGG | 0.253571 | 8.1:+5459917 | None:intergenic |
| TGTCACAAATAGATTTGTTA+TGG | 0.306145 | 8.1:-5460063 | MS.gene073317:CDS |
| AGAAAATCAGATATCAAGTT+AGG | 0.387014 | 8.1:-5459961 | MS.gene073317:CDS |
| GTCACAAATAGATTTGTTAT+GGG | 0.395534 | 8.1:-5460062 | MS.gene073317:CDS |
| TTGTTATGGGAGAATGGAGG+AGG | 0.440896 | 8.1:-5460049 | MS.gene073317:CDS |
| ATAGATTTGTTATGGGAGAA+TGG | 0.448843 | 8.1:-5460055 | MS.gene073317:CDS |
| TGGGAGAATGGAGGAGGAAG+TGG | 0.459617 | 8.1:-5460043 | MS.gene073317:CDS |
| TTCTTTGTAACGCAGCACGC+TGG | 0.571082 | 8.1:+5460009 | None:intergenic |
| AGATCGACGGCCAAGAATGA+AGG | 0.572545 | 8.1:-5459927 | None:intergenic |
| AATCAGATATCAAGTTAGGA+AGG | 0.579412 | 8.1:-5459957 | MS.gene073317:CDS |
| GATTTGTTATGGGAGAATGG+AGG | 0.628311 | 8.1:-5460052 | MS.gene073317:CDS |
| GGAAGGTCAATGCAGATCGA+CGG | 0.632746 | 8.1:-5459940 | MS.gene073317:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAAAATCAGATATCAAGTT+AGG | - | chr8.1:5460037-5460056 | MS.gene073317:CDS | 25.0% |
| !!! | GTCACAAATAGATTTGTTAT+GGG | - | chr8.1:5459936-5459955 | MS.gene073317:CDS | 25.0% |
| !!! | TGTCACAAATAGATTTGTTA+TGG | - | chr8.1:5459935-5459954 | MS.gene073317:CDS | 25.0% |
| AATCAGATATCAAGTTAGGA+AGG | - | chr8.1:5460041-5460060 | MS.gene073317:CDS | 30.0% | |
| ! | ATCTGATTTTCTTAGAGATG+AGG | + | chr8.1:5460029-5460048 | None:intergenic | 30.0% |
| !! | ATAGATTTGTTATGGGAGAA+TGG | - | chr8.1:5459943-5459962 | MS.gene073317:CDS | 30.0% |
| !! | GATTTGTTATGGGAGAATGG+AGG | - | chr8.1:5459946-5459965 | MS.gene073317:CDS | 40.0% |
| TTGTTATGGGAGAATGGAGG+AGG | - | chr8.1:5459949-5459968 | MS.gene073317:CDS | 45.0% | |
| AGATCGACGGCCAAGAATGA+AGG | - | chr8.1:5460071-5460090 | MS.gene073317:CDS | 50.0% | |
| GGAAGGTCAATGCAGATCGA+CGG | - | chr8.1:5460058-5460077 | MS.gene073317:CDS | 50.0% | |
| TTCTTTGTAACGCAGCACGC+TGG | + | chr8.1:5459992-5460011 | None:intergenic | 50.0% | |
| TGGGAGAATGGAGGAGGAAG+TGG | - | chr8.1:5459955-5459974 | MS.gene073317:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 5459928 | 5460092 | 5459928 | ID=MS.gene073317 |
| chr8.1 | mRNA | 5459928 | 5460092 | 5459928 | ID=MS.gene073317.t1;Parent=MS.gene073317 |
| chr8.1 | exon | 5459928 | 5460092 | 5459928 | ID=MS.gene073317.t1.exon1;Parent=MS.gene073317.t1 |
| chr8.1 | CDS | 5459928 | 5460092 | 5459928 | ID=cds.MS.gene073317.t1;Parent=MS.gene073317.t1 |
| Gene Sequence |
| Protein sequence |
