Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43355.t1 | XP_013467255.1 | 89 | 353 | 39 | 0 | 1 | 353 | 1 | 353 | 2.00E-161 | 578.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43355.t1 | Q84W65 | 61.1 | 375 | 117 | 8 | 1 | 350 | 1 | 371 | 9.7e-104 | 378.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43355.t1 | A0A072VGX0 | 89.0 | 353 | 39 | 0 | 1 | 353 | 1 | 353 | 1.5e-161 | 578.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene43355 | 0.801079 | 1.07E-48 | -1.69E-46 |
| MS.gene049171 | MS.gene43355 | 0.82681 | 2.21E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene43355 | 0.853626 | 2.11E-61 | -1.69E-46 |
| MS.gene049285 | MS.gene43355 | 0.818936 | 1.51E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene43355 | 0.81285 | 3.44E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene43355 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene049627 | MS.gene43355 | 0.812233 | 4.70E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene43355 | 0.810167 | 1.32E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene43355 | 0.806779 | 6.97E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene43355 | 0.830165 | 3.42E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene43355 | 0.801692 | 7.99E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene43355 | 0.844245 | 8.52E-59 | -1.69E-46 |
| MS.gene050518 | MS.gene43355 | 0.81318 | 2.92E-51 | -1.69E-46 |
| MS.gene050538 | MS.gene43355 | 0.822565 | 2.21E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene43355 | 0.88659 | 3.00E-72 | -1.69E-46 |
| MS.gene050612 | MS.gene43355 | 0.818399 | 2.00E-52 | -1.69E-46 |
| MS.gene050699 | MS.gene43355 | 0.820114 | 8.14E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene43355 | 0.877582 | 5.60E-69 | -1.69E-46 |
| MS.gene050772 | MS.gene43355 | 0.809567 | 1.78E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene43355 | 0.815905 | 7.27E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene43355 | 0.820311 | 7.34E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene43355 | 0.821302 | 4.34E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene43355 | 0.82321 | 1.57E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene43355 | 0.871193 | 8.24E-67 | -1.69E-46 |
| MS.gene050986 | MS.gene43355 | 0.810235 | 1.27E-50 | -1.69E-46 |
| MS.gene05100 | MS.gene43355 | 0.810011 | 1.42E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene43355 | 0.806542 | 7.82E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene43355 | 0.807652 | 4.55E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene43355 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene43355 | 0.826796 | 2.23E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene43355 | 0.879888 | 8.62E-70 | -1.69E-46 |
| MS.gene052482 | MS.gene43355 | 0.84252 | 2.46E-58 | -1.69E-46 |
| MS.gene052486 | MS.gene43355 | 0.856205 | 3.76E-62 | -1.69E-46 |
| MS.gene052494 | MS.gene43355 | -0.817091 | 3.95E-52 | -1.69E-46 |
| MS.gene052501 | MS.gene43355 | 0.818513 | 1.88E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene43355 | 0.844026 | 9.75E-59 | -1.69E-46 |
| MS.gene052505 | MS.gene43355 | 0.807381 | 5.20E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene43355 | 0.831468 | 1.64E-55 | -1.69E-46 |
| MS.gene052823 | MS.gene43355 | 0.845521 | 3.86E-59 | -1.69E-46 |
| MS.gene052825 | MS.gene43355 | 0.848984 | 4.33E-60 | -1.69E-46 |
| MS.gene052966 | MS.gene43355 | 0.822927 | 1.82E-53 | -1.69E-46 |
| MS.gene052982 | MS.gene43355 | 0.822954 | 1.80E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene43355 | 0.85346 | 2.36E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene43355 | 0.807971 | 3.90E-50 | -1.69E-46 |
| MS.gene053213 | MS.gene43355 | 0.827651 | 1.39E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene43355 | 0.831821 | 1.34E-55 | -1.69E-46 |
| MS.gene053436 | MS.gene43355 | 0.871339 | 7.37E-67 | -1.69E-46 |
| MS.gene053480 | MS.gene43355 | 0.801701 | 7.95E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene43355 | 0.844854 | 5.84E-59 | -1.69E-46 |
| MS.gene053579 | MS.gene43355 | 0.801085 | 1.06E-48 | -1.69E-46 |
| MS.gene053626 | MS.gene43355 | 0.850923 | 1.24E-60 | -1.69E-46 |
| MS.gene053632 | MS.gene43355 | 0.806419 | 8.30E-50 | -1.69E-46 |
| MS.gene054408 | MS.gene43355 | 0.852174 | 5.49E-61 | -1.69E-46 |
| MS.gene054595 | MS.gene43355 | 0.836501 | 9.02E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene43355 | 0.81583 | 7.56E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene43355 | 0.803 | 4.29E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene43355 | 0.857305 | 1.79E-62 | -1.69E-46 |
| MS.gene054927 | MS.gene43355 | 0.831701 | 1.44E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene43355 | 0.815128 | 1.08E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene43355 | 0.824361 | 8.41E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene43355 | 0.834532 | 2.84E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene43355 | 0.846068 | 2.74E-59 | -1.69E-46 |
| MS.gene055629 | MS.gene43355 | 0.804276 | 2.33E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene43355 | 0.852928 | 3.34E-61 | -1.69E-46 |
| MS.gene055750 | MS.gene43355 | 0.840847 | 6.79E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene43355 | 0.834198 | 3.44E-56 | -1.69E-46 |
| MS.gene05589 | MS.gene43355 | 0.84086 | 6.74E-58 | -1.69E-46 |
| MS.gene056040 | MS.gene43355 | 0.880996 | 3.46E-70 | -1.69E-46 |
| MS.gene056166 | MS.gene43355 | 0.827715 | 1.34E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene43355 | 0.811767 | 5.94E-51 | -1.69E-46 |
| MS.gene056409 | MS.gene43355 | 0.819872 | 9.24E-53 | -1.69E-46 |
| MS.gene056450 | MS.gene43355 | 0.800064 | 1.72E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene43355 | 0.83109 | 2.03E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene43355 | 0.824575 | 7.49E-54 | -1.69E-46 |
| MS.gene056610 | MS.gene43355 | 0.827544 | 1.48E-54 | -1.69E-46 |
| MS.gene056647 | MS.gene43355 | -0.805788 | 1.13E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene43355 | 0.814898 | 1.22E-51 | -1.69E-46 |
| MS.gene05666 | MS.gene43355 | 0.806064 | 9.86E-50 | -1.69E-46 |
| MS.gene056692 | MS.gene43355 | 0.826118 | 3.23E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene43355 | 0.805811 | 1.11E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene43355 | 0.833548 | 5.01E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene43355 | 0.853042 | 3.10E-61 | -1.69E-46 |
| MS.gene05787 | MS.gene43355 | 0.844963 | 5.46E-59 | -1.69E-46 |
| MS.gene058130 | MS.gene43355 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene43355 | 0.804973 | 1.67E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene43355 | 0.84179 | 3.84E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene43355 | 0.811502 | 6.78E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene43355 | 0.855576 | 5.75E-62 | -1.69E-46 |
| MS.gene058838 | MS.gene43355 | 0.855127 | 7.77E-62 | -1.69E-46 |
| MS.gene05883 | MS.gene43355 | 0.820338 | 7.23E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene43355 | 0.815765 | 7.82E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene43355 | 0.841221 | 5.42E-58 | -1.69E-46 |
| MS.gene059103 | MS.gene43355 | -0.80123 | 9.93E-49 | -1.69E-46 |
| MS.gene059303 | MS.gene43355 | 0.813714 | 2.23E-51 | -1.69E-46 |
| MS.gene059480 | MS.gene43355 | -0.800115 | 1.68E-48 | -1.69E-46 |
| MS.gene059667 | MS.gene43355 | 0.839291 | 1.73E-57 | -1.69E-46 |
| MS.gene059677 | MS.gene43355 | 0.804887 | 1.74E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene43355 | 0.842151 | 3.08E-58 | -1.69E-46 |
| MS.gene059756 | MS.gene43355 | 0.805252 | 1.46E-49 | -1.69E-46 |
| MS.gene059801 | MS.gene43355 | 0.829805 | 4.19E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene43355 | 0.816895 | 4.37E-52 | -1.69E-46 |
| MS.gene06000 | MS.gene43355 | 0.837666 | 4.54E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene43355 | 0.830667 | 2.58E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene43355 | 0.804759 | 1.85E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene43355 | 0.862965 | 3.49E-64 | -1.69E-46 |
| MS.gene06092 | MS.gene43355 | 0.836452 | 9.28E-57 | -1.69E-46 |
| MS.gene060982 | MS.gene43355 | 0.8577 | 1.36E-62 | -1.69E-46 |
| MS.gene061150 | MS.gene43355 | 0.837143 | 6.18E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43355.t1 | MTR_1g047990 | 96.034 | 353 | 14 | 0 | 1 | 353 | 1 | 353 | 0.0 | 687 |
| MS.gene43355.t1 | MTR_6g079650 | 57.609 | 92 | 38 | 1 | 260 | 350 | 9 | 100 | 1.15e-31 | 115 |
| MS.gene43355.t1 | MTR_4g104680 | 36.000 | 200 | 117 | 7 | 16 | 213 | 4 | 194 | 1.00e-21 | 97.1 |
| MS.gene43355.t1 | MTR_3g086590 | 35.938 | 128 | 80 | 1 | 78 | 203 | 61 | 188 | 1.49e-18 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43355.t1 | AT4G26500 | 70.847 | 295 | 73 | 4 | 69 | 350 | 77 | 371 | 8.73e-136 | 391 |
| MS.gene43355.t1 | AT1G55805 | 71.591 | 88 | 24 | 1 | 263 | 349 | 68 | 155 | 9.87e-40 | 138 |
| MS.gene43355.t1 | AT1G67810 | 31.429 | 175 | 113 | 3 | 40 | 208 | 35 | 208 | 1.20e-16 | 79.3 |
| MS.gene43355.t1 | AT5G50210 | 32.812 | 192 | 124 | 4 | 24 | 213 | 35 | 223 | 1.14e-14 | 75.9 |
Find 92 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAAGATGAGTTGGATTC+TGG | 0.176580 | 1.4:+33060973 | MS.gene43355:CDS |
| CCAAGTTTAGGTGTTGATTT+TGG | 0.250352 | 1.4:+33060715 | MS.gene43355:CDS |
| GAAGAGAAGCTGTTCGTATT+TGG | 0.258831 | 1.4:-33060269 | None:intergenic |
| TTAACCCCTTCTAGAAATAA+TGG | 0.260107 | 1.4:+33060544 | MS.gene43355:CDS |
| GAAGAGGGAAAGTGAGAATT+TGG | 0.282002 | 1.4:-33060038 | None:intergenic |
| AAGGTTGTGTCTCTCAAGTT+TGG | 0.310231 | 1.4:+33060350 | MS.gene43355:CDS |
| AGCTGTTCGTATTTGGCTTT+GGG | 0.314164 | 1.4:-33060262 | None:intergenic |
| AAGCTGTTCGTATTTGGCTT+TGG | 0.330510 | 1.4:-33060263 | None:intergenic |
| CCCAAGATGCATTACAAAAT+CGG | 0.341009 | 1.4:-33060509 | None:intergenic |
| TTTACGATTTCTTGAAGCTT+TGG | 0.349699 | 1.4:-33060217 | None:intergenic |
| CCTTCTGTGACCCCAAGTTT+AGG | 0.359965 | 1.4:+33060703 | MS.gene43355:CDS |
| AGGGAGAGGGAAGAGGATAA+AGG | 0.373167 | 1.4:+33060771 | MS.gene43355:CDS |
| CTCGCTGCTTTACTCGTTCA+AGG | 0.382796 | 1.4:+33060445 | MS.gene43355:CDS |
| AGAGTTTCAAGGGAAGAGTT+TGG | 0.384288 | 1.4:+33060921 | MS.gene43355:CDS |
| TAACCCCTTCTAGAAATAAT+GGG | 0.387357 | 1.4:+33060545 | MS.gene43355:CDS |
| GCTTTATGTGGAAGCTGAAA+AGG | 0.396625 | 1.4:+33060609 | MS.gene43355:CDS |
| TGATGACGAGAATGTTGAAT+TGG | 0.400400 | 1.4:+33060747 | MS.gene43355:CDS |
| TGGGAGTGATTGGAAGGTGA+TGG | 0.400836 | 1.4:-33060119 | None:intergenic |
| ACCTGGATAATTTCATTAAC+GGG | 0.402796 | 1.4:-33060481 | None:intergenic |
| CGTTGTCTATGAAGCTGATT+CGG | 0.403821 | 1.4:+33060402 | MS.gene43355:CDS |
| CTTTGGGTTCTTGCACGGAT+TGG | 0.404849 | 1.4:-33060246 | None:intergenic |
| AAGGGAAGAGTTTGGTTAAG+AGG | 0.410584 | 1.4:+33060929 | MS.gene43355:CDS |
| ATTGGGATTCGAGGGGTTTG+AGG | 0.413363 | 1.4:-33060303 | None:intergenic |
| AGGTTGTGTCTCTCAAGTTT+GGG | 0.420021 | 1.4:+33060351 | MS.gene43355:CDS |
| TCTTCAACTTCCAACTCAAT+AGG | 0.421778 | 1.4:-33060817 | None:intergenic |
| GACCTGGATAATTTCATTAA+CGG | 0.427963 | 1.4:-33060482 | None:intergenic |
| ACTTCTGAATCTGACTCAAT+TGG | 0.431624 | 1.4:+33060634 | MS.gene43355:CDS |
| CCAAAATCAACACCTAAACT+TGG | 0.437746 | 1.4:-33060715 | None:intergenic |
| GGGAGTGATTGGAAGGTGAT+GGG | 0.438845 | 1.4:-33060118 | None:intergenic |
| AAAAGCACTTATGCTTTATG+TGG | 0.444787 | 1.4:+33060597 | MS.gene43355:CDS |
| TCGGATTCTGTTCTCGCCAA+AGG | 0.444866 | 1.4:+33060421 | MS.gene43355:CDS |
| TTCGATGGGTTGAAGGGAAG+TGG | 0.449871 | 1.4:-33060185 | None:intergenic |
| GGAGTTCTTCGATGGGTTGA+AGG | 0.449918 | 1.4:-33060192 | None:intergenic |
| AATTGGGAGGGAGAGGGAAG+AGG | 0.450448 | 1.4:+33060764 | MS.gene43355:CDS |
| GAAAGAGAAGAGGGTGTTTG+TGG | 0.454312 | 1.4:-33060092 | None:intergenic |
| AAAACCCATTATTTCTAGAA+GGG | 0.455596 | 1.4:-33060549 | None:intergenic |
| TGAACATGAAGAAAGGGAAT+TGG | 0.456159 | 1.4:-33059999 | None:intergenic |
| GTGGTTTCTAAAGAGTTTCA+AGG | 0.456838 | 1.4:+33060910 | MS.gene43355:CDS |
| CTTTGGAGGGAGTTCTTCGA+TGG | 0.457099 | 1.4:-33060200 | None:intergenic |
| TAGCTTGCTTCAAGATGAGT+TGG | 0.460976 | 1.4:+33060966 | MS.gene43355:CDS |
| CGGATTCTGTTCTCGCCAAA+GGG | 0.463295 | 1.4:+33060422 | MS.gene43355:CDS |
| AAAGAGAAGAGGGTGTTTGT+GGG | 0.468262 | 1.4:-33060091 | None:intergenic |
| GGAACTTCAGCCTATTGAGT+TGG | 0.469218 | 1.4:+33060807 | MS.gene43355:CDS |
| ATCGAAATGAACATGAAGAA+AGG | 0.469710 | 1.4:-33060006 | None:intergenic |
| AGTTTGGGTCCGAGCCTACT+TGG | 0.471796 | 1.4:+33060366 | MS.gene43355:CDS |
| GATGATGATGATGGTGGTGG+TGG | 0.473775 | 1.4:-33060148 | None:intergenic |
| TTTGGCTTTGGGTTCTTGCA+CGG | 0.474908 | 1.4:-33060251 | None:intergenic |
| CTGGACATGCTGGTGTTAGA+AGG | 0.483576 | 1.4:+33060857 | MS.gene43355:CDS |
| ACGATTTCTTGAAGCTTTGG+AGG | 0.485000 | 1.4:-33060214 | None:intergenic |
| CCAAGATGCATTACAAAATC+GGG | 0.485227 | 1.4:-33060508 | None:intergenic |
| GACACATTTCAATGTTAAAG+TGG | 0.486500 | 1.4:+33060891 | MS.gene43355:CDS |
| AATGTTGAATTGGGAGGGAG+AGG | 0.486652 | 1.4:+33060757 | MS.gene43355:CDS |
| TACGAACAGCTTCTCTTCTA+CGG | 0.489334 | 1.4:+33060274 | MS.gene43355:CDS |
| GATGACGAGAATGTTGAATT+GGG | 0.497878 | 1.4:+33060748 | MS.gene43355:CDS |
| GATAAAGGAAAAGCTTGAAA+AGG | 0.498122 | 1.4:+33060786 | MS.gene43355:CDS |
| ACCGACTTCAGAAGGCGTCT+TGG | 0.500732 | 1.4:-33061019 | None:intergenic |
| AGAGTTTGGTTAAGAGGCAT+AGG | 0.503534 | 1.4:+33060935 | MS.gene43355:CDS |
| TGAAGGGAAGTGGATGGAAG+TGG | 0.504888 | 1.4:-33060175 | None:intergenic |
| CTTTATGTGGAAGCTGAAAA+GGG | 0.506897 | 1.4:+33060610 | MS.gene43355:CDS |
| TTACAAAATCGGGTGTGACC+TGG | 0.509018 | 1.4:-33060498 | None:intergenic |
| CATCCTTCACCGACTTCAGA+AGG | 0.509950 | 1.4:-33061027 | None:intergenic |
| TCGAAATGAACATGAAGAAA+GGG | 0.512101 | 1.4:-33060005 | None:intergenic |
| GTTCTTGCACGGATTGGAAG+AGG | 0.512154 | 1.4:-33060240 | None:intergenic |
| CAAAATCAACACCTAAACTT+GGG | 0.519100 | 1.4:-33060714 | None:intergenic |
| TATCAACATGCTGGACATGC+TGG | 0.527867 | 1.4:+33060847 | MS.gene43355:CDS |
| GAGTAAAGCAGCGAGCCCTT+TGG | 0.537959 | 1.4:-33060437 | None:intergenic |
| ACATGAAGAAAGGGAATTGG+TGG | 0.538024 | 1.4:-33059996 | None:intergenic |
| ATGTTGAATTGGGAGGGAGA+GGG | 0.542038 | 1.4:+33060758 | MS.gene43355:CDS |
| AAACCCATTATTTCTAGAAG+GGG | 0.542885 | 1.4:-33060548 | None:intergenic |
| GATGGGTTTGAAAGAGAAGA+GGG | 0.545338 | 1.4:-33060101 | None:intergenic |
| ACCCGTTAATGAAATTATCC+AGG | 0.545924 | 1.4:+33060480 | MS.gene43355:CDS |
| CCTAAACTTGGGGTCACAGA+AGG | 0.548902 | 1.4:-33060703 | None:intergenic |
| CGATTTCTTGAAGCTTTGGA+GGG | 0.549690 | 1.4:-33060213 | None:intergenic |
| ATGGGTTGAAGGGAAGTGGA+TGG | 0.556200 | 1.4:-33060181 | None:intergenic |
| AGCTTTGTTGAGAATTCAAG+TGG | 0.556883 | 1.4:+33060679 | MS.gene43355:CDS |
| TGGGTCCGAGCCTACTTGGA+TGG | 0.557259 | 1.4:+33060370 | MS.gene43355:CDS |
| GTTTCTTTCACCATCCAAGT+AGG | 0.562090 | 1.4:-33060380 | None:intergenic |
| ACGAGAATGTTGAATTGGGA+GGG | 0.562350 | 1.4:+33060752 | MS.gene43355:CDS |
| GCCAAGACGCCTTCTGAAGT+CGG | 0.570403 | 1.4:+33061018 | MS.gene43355:CDS |
| TGGTTTCTAAAGAGTTTCAA+GGG | 0.574771 | 1.4:+33060911 | MS.gene43355:CDS |
| TGATGGGTTTGAAAGAGAAG+AGG | 0.578500 | 1.4:-33060102 | None:intergenic |
| TTTCACCATCCAAGTAGGCT+CGG | 0.579472 | 1.4:-33060375 | None:intergenic |
| GAGTTCTTCGATGGGTTGAA+GGG | 0.581123 | 1.4:-33060191 | None:intergenic |
| ACGCCTTCTGAAGTCGGTGA+AGG | 0.585013 | 1.4:+33061024 | MS.gene43355:CDS |
| TTTGGAGGGAGTTCTTCGAT+GGG | 0.593786 | 1.4:-33060199 | None:intergenic |
| GATGTGTCTTATCAACATGC+TGG | 0.597053 | 1.4:+33060838 | MS.gene43355:CDS |
| AACATTCTCGTCATCAACCT+CGG | 0.597911 | 1.4:-33060740 | None:intergenic |
| AAAATCAACACCTAAACTTG+GGG | 0.630526 | 1.4:-33060713 | None:intergenic |
| CTAAACTTGGGGTCACAGAA+GGG | 0.633009 | 1.4:-33060702 | None:intergenic |
| ACTAACGACAACAAAGTCGA+AGG | 0.640734 | 1.4:+33060331 | MS.gene43355:CDS |
| GACGAGAATGTTGAATTGGG+AGG | 0.654890 | 1.4:+33060751 | MS.gene43355:CDS |
| GCTGGTGTTAGAAGGAGCGA+TGG | 0.690557 | 1.4:+33060865 | MS.gene43355:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAACCCATTATTTCTAGA+AGG | - | chr1.4:33060553-33060572 | None:intergenic | 25.0% |
| !!! | AAAACCCATTATTTCTAGAA+GGG | - | chr1.4:33060552-33060571 | None:intergenic | 25.0% |
| !!! | AGAGTTATTTTTGTTGAAGA+GGG | - | chr1.4:33060056-33060075 | None:intergenic | 25.0% |
| !!! | GTTGTCGTTAGTTTTAAATT+GGG | - | chr1.4:33060323-33060342 | None:intergenic | 25.0% |
| !!! | TAGAGTTATTTTTGTTGAAG+AGG | - | chr1.4:33060057-33060076 | None:intergenic | 25.0% |
| !!! | TGTTGTCGTTAGTTTTAAAT+TGG | - | chr1.4:33060324-33060343 | None:intergenic | 25.0% |
| AAAATCAACACCTAAACTTG+GGG | - | chr1.4:33060716-33060735 | None:intergenic | 30.0% | |
| ACCTGGATAATTTCATTAAC+GGG | - | chr1.4:33060484-33060503 | None:intergenic | 30.0% | |
| ATCGAAATGAACATGAAGAA+AGG | - | chr1.4:33060009-33060028 | None:intergenic | 30.0% | |
| CAAAATCAACACCTAAACTT+GGG | - | chr1.4:33060717-33060736 | None:intergenic | 30.0% | |
| GACACATTTCAATGTTAAAG+TGG | + | chr1.4:33060891-33060910 | MS.gene43355:CDS | 30.0% | |
| GACCTGGATAATTTCATTAA+CGG | - | chr1.4:33060485-33060504 | None:intergenic | 30.0% | |
| GATAAAGGAAAAGCTTGAAA+AGG | + | chr1.4:33060786-33060805 | MS.gene43355:CDS | 30.0% | |
| TCGAAATGAACATGAAGAAA+GGG | - | chr1.4:33060008-33060027 | None:intergenic | 30.0% | |
| TTTACGATTTCTTGAAGCTT+TGG | - | chr1.4:33060220-33060239 | None:intergenic | 30.0% | |
| ! | TAACCCCTTCTAGAAATAAT+GGG | + | chr1.4:33060545-33060564 | MS.gene43355:CDS | 30.0% |
| ! | TGGTTTCTAAAGAGTTTCAA+GGG | + | chr1.4:33060911-33060930 | MS.gene43355:CDS | 30.0% |
| ! | TTAACCCCTTCTAGAAATAA+TGG | + | chr1.4:33060544-33060563 | MS.gene43355:CDS | 30.0% |
| !! | AAAAGCACTTATGCTTTATG+TGG | + | chr1.4:33060597-33060616 | MS.gene43355:CDS | 30.0% |
| !! | AAACCCATTATTTCTAGAAG+GGG | - | chr1.4:33060551-33060570 | None:intergenic | 30.0% |
| !!! | AGTTTTAAATTGGGATTCGA+GGG | - | chr1.4:33060314-33060333 | None:intergenic | 30.0% |
| !!! | TAGTTTTAAATTGGGATTCG+AGG | - | chr1.4:33060315-33060334 | None:intergenic | 30.0% |
| ACCCGTTAATGAAATTATCC+AGG | + | chr1.4:33060480-33060499 | MS.gene43355:CDS | 35.0% | |
| ACTTCTGAATCTGACTCAAT+TGG | + | chr1.4:33060634-33060653 | MS.gene43355:CDS | 35.0% | |
| CCAAAATCAACACCTAAACT+TGG | - | chr1.4:33060718-33060737 | None:intergenic | 35.0% | |
| CCAAGATGCATTACAAAATC+GGG | - | chr1.4:33060511-33060530 | None:intergenic | 35.0% | |
| CCCAAGATGCATTACAAAAT+CGG | - | chr1.4:33060512-33060531 | None:intergenic | 35.0% | |
| CTTTATGTGGAAGCTGAAAA+GGG | + | chr1.4:33060610-33060629 | MS.gene43355:CDS | 35.0% | |
| GATGACGAGAATGTTGAATT+GGG | + | chr1.4:33060748-33060767 | MS.gene43355:CDS | 35.0% | |
| TCTTCAACTTCCAACTCAAT+AGG | - | chr1.4:33060820-33060839 | None:intergenic | 35.0% | |
| TGAACATGAAGAAAGGGAAT+TGG | - | chr1.4:33060002-33060021 | None:intergenic | 35.0% | |
| ! | CCGATTTTGTAATGCATCTT+GGG | + | chr1.4:33060509-33060528 | MS.gene43355:CDS | 35.0% |
| ! | GTGGTTTCTAAAGAGTTTCA+AGG | + | chr1.4:33060910-33060929 | MS.gene43355:CDS | 35.0% |
| ! | TGATGACGAGAATGTTGAAT+TGG | + | chr1.4:33060747-33060766 | MS.gene43355:CDS | 35.0% |
| !! | AGCTTTGTTGAGAATTCAAG+TGG | + | chr1.4:33060679-33060698 | MS.gene43355:CDS | 35.0% |
| !! | GTTTTAAATTGGGATTCGAG+GGG | - | chr1.4:33060313-33060332 | None:intergenic | 35.0% |
| !!! | CCAAGTTTAGGTGTTGATTT+TGG | + | chr1.4:33060715-33060734 | MS.gene43355:CDS | 35.0% |
| AACATTCTCGTCATCAACCT+CGG | - | chr1.4:33060743-33060762 | None:intergenic | 40.0% | |
| AAGGGAAGAGTTTGGTTAAG+AGG | + | chr1.4:33060929-33060948 | MS.gene43355:CDS | 40.0% | |
| AAGGTTGTGTCTCTCAAGTT+TGG | + | chr1.4:33060350-33060369 | MS.gene43355:CDS | 40.0% | |
| ACATGAAGAAAGGGAATTGG+TGG | - | chr1.4:33059999-33060018 | None:intergenic | 40.0% | |
| ACGAGAATGTTGAATTGGGA+GGG | + | chr1.4:33060752-33060771 | MS.gene43355:CDS | 40.0% | |
| ACTAACGACAACAAAGTCGA+AGG | + | chr1.4:33060331-33060350 | MS.gene43355:CDS | 40.0% | |
| AGAGTTTCAAGGGAAGAGTT+TGG | + | chr1.4:33060921-33060940 | MS.gene43355:CDS | 40.0% | |
| AGGTTGTGTCTCTCAAGTTT+GGG | + | chr1.4:33060351-33060370 | MS.gene43355:CDS | 40.0% | |
| CGTTGTCTATGAAGCTGATT+CGG | + | chr1.4:33060402-33060421 | MS.gene43355:CDS | 40.0% | |
| GAAGAGAAGCTGTTCGTATT+TGG | - | chr1.4:33060272-33060291 | None:intergenic | 40.0% | |
| GAAGAGGGAAAGTGAGAATT+TGG | - | chr1.4:33060041-33060060 | None:intergenic | 40.0% | |
| GATGTGTCTTATCAACATGC+TGG | + | chr1.4:33060838-33060857 | MS.gene43355:CDS | 40.0% | |
| GCTTTATGTGGAAGCTGAAA+AGG | + | chr1.4:33060609-33060628 | MS.gene43355:CDS | 40.0% | |
| GTTTCTTTCACCATCCAAGT+AGG | - | chr1.4:33060383-33060402 | None:intergenic | 40.0% | |
| TACGAACAGCTTCTCTTCTA+CGG | + | chr1.4:33060274-33060293 | MS.gene43355:CDS | 40.0% | |
| ! | AAGCTGTTCGTATTTGGCTT+TGG | - | chr1.4:33060266-33060285 | None:intergenic | 40.0% |
| ! | ACGATTTCTTGAAGCTTTGG+AGG | - | chr1.4:33060217-33060236 | None:intergenic | 40.0% |
| ! | AGAGTTTGGTTAAGAGGCAT+AGG | + | chr1.4:33060935-33060954 | MS.gene43355:CDS | 40.0% |
| ! | AGTGGTGATGATGATGATGA+TGG | - | chr1.4:33060160-33060179 | None:intergenic | 40.0% |
| ! | CCCGATTTTGTAATGCATCT+TGG | + | chr1.4:33060508-33060527 | MS.gene43355:CDS | 40.0% |
| ! | CTTCAAGATGAGTTGGATTC+TGG | + | chr1.4:33060973-33060992 | MS.gene43355:CDS | 40.0% |
| ! | GATGGGTTTGAAAGAGAAGA+GGG | - | chr1.4:33060104-33060123 | None:intergenic | 40.0% |
| ! | TTACTCGTTCAAGGCTTTTC+AGG | + | chr1.4:33060454-33060473 | MS.gene43355:CDS | 40.0% |
| ! | TTTTGTTGGGAGTGATTGGA+AGG | - | chr1.4:33060128-33060147 | None:intergenic | 40.0% |
| !! | AAAGAGAAGAGGGTGTTTGT+GGG | - | chr1.4:33060094-33060113 | None:intergenic | 40.0% |
| !! | AGCTGTTCGTATTTGGCTTT+GGG | - | chr1.4:33060265-33060284 | None:intergenic | 40.0% |
| !! | CGATTTCTTGAAGCTTTGGA+GGG | - | chr1.4:33060216-33060235 | None:intergenic | 40.0% |
| !! | TAGCTTGCTTCAAGATGAGT+TGG | + | chr1.4:33060966-33060985 | MS.gene43355:CDS | 40.0% |
| !! | TGATGGGTTTGAAAGAGAAG+AGG | - | chr1.4:33060105-33060124 | None:intergenic | 40.0% |
| AATGTTGAATTGGGAGGGAG+AGG | + | chr1.4:33060757-33060776 | MS.gene43355:CDS | 45.0% | |
| ATGTTGAATTGGGAGGGAGA+GGG | + | chr1.4:33060758-33060777 | MS.gene43355:CDS | 45.0% | |
| CTAAACTTGGGGTCACAGAA+GGG | - | chr1.4:33060705-33060724 | None:intergenic | 45.0% | |
| GACGAGAATGTTGAATTGGG+AGG | + | chr1.4:33060751-33060770 | MS.gene43355:CDS | 45.0% | |
| GGAACTTCAGCCTATTGAGT+TGG | + | chr1.4:33060807-33060826 | MS.gene43355:CDS | 45.0% | |
| TATCAACATGCTGGACATGC+TGG | + | chr1.4:33060847-33060866 | MS.gene43355:CDS | 45.0% | |
| TTACAAAATCGGGTGTGACC+TGG | - | chr1.4:33060501-33060520 | None:intergenic | 45.0% | |
| TTTCACCATCCAAGTAGGCT+CGG | - | chr1.4:33060378-33060397 | None:intergenic | 45.0% | |
| TTTGGAGGGAGTTCTTCGAT+GGG | - | chr1.4:33060202-33060221 | None:intergenic | 45.0% | |
| !! | GAAAGAGAAGAGGGTGTTTG+TGG | - | chr1.4:33060095-33060114 | None:intergenic | 45.0% |
| !! | GAGTTCTTCGATGGGTTGAA+GGG | - | chr1.4:33060194-33060213 | None:intergenic | 45.0% |
| !! | GATGATGATGATGATGGTGG+TGG | - | chr1.4:33060154-33060173 | None:intergenic | 45.0% |
| !! | GGTGATGATGATGATGATGG+TGG | - | chr1.4:33060157-33060176 | None:intergenic | 45.0% |
| !! | TTTGGCTTTGGGTTCTTGCA+CGG | - | chr1.4:33060254-33060273 | None:intergenic | 45.0% |
| !!! | AGGTGTTGATTTTGGTTCCG+AGG | + | chr1.4:33060723-33060742 | MS.gene43355:CDS | 45.0% |
| !!! | ATGGTGGTGGTGGTTTTGTT+GGG | - | chr1.4:33060141-33060160 | None:intergenic | 45.0% |
| !!! | GTGGTTTTGTTGGGAGTGAT+TGG | - | chr1.4:33060132-33060151 | None:intergenic | 45.0% |
| AGGGAGAGGGAAGAGGATAA+AGG | + | chr1.4:33060771-33060790 | MS.gene43355:CDS | 50.0% | |
| ATTGGGATTCGAGGGGTTTG+AGG | - | chr1.4:33060306-33060325 | None:intergenic | 50.0% | |
| CCTAAACTTGGGGTCACAGA+AGG | - | chr1.4:33060706-33060725 | None:intergenic | 50.0% | |
| CCTTCTGTGACCCCAAGTTT+AGG | + | chr1.4:33060703-33060722 | MS.gene43355:CDS | 50.0% | |
| CGGATTCTGTTCTCGCCAAA+GGG | + | chr1.4:33060422-33060441 | MS.gene43355:CDS | 50.0% | |
| GGGAGTGATTGGAAGGTGAT+GGG | - | chr1.4:33060121-33060140 | None:intergenic | 50.0% | |
| TCGGATTCTGTTCTCGCCAA+AGG | + | chr1.4:33060421-33060440 | MS.gene43355:CDS | 50.0% | |
| TGAAGGGAAGTGGATGGAAG+TGG | - | chr1.4:33060178-33060197 | None:intergenic | 50.0% | |
| TGGGAGTGATTGGAAGGTGA+TGG | - | chr1.4:33060122-33060141 | None:intergenic | 50.0% | |
| ! | ATGGGTTGAAGGGAAGTGGA+TGG | - | chr1.4:33060184-33060203 | None:intergenic | 50.0% |
| ! | CTCGCTGCTTTACTCGTTCA+AGG | + | chr1.4:33060445-33060464 | MS.gene43355:CDS | 50.0% |
| ! | CTGGACATGCTGGTGTTAGA+AGG | + | chr1.4:33060857-33060876 | MS.gene43355:CDS | 50.0% |
| ! | CTTTGGAGGGAGTTCTTCGA+TGG | - | chr1.4:33060203-33060222 | None:intergenic | 50.0% |
| ! | CTTTGGGTTCTTGCACGGAT+TGG | - | chr1.4:33060249-33060268 | None:intergenic | 50.0% |
| !! | CATCCTTCACCGACTTCAGA+AGG | - | chr1.4:33061030-33061049 | None:intergenic | 50.0% |
| !! | GATGATGATGATGGTGGTGG+TGG | - | chr1.4:33060151-33060170 | None:intergenic | 50.0% |
| !! | GGAGTTCTTCGATGGGTTGA+AGG | - | chr1.4:33060195-33060214 | None:intergenic | 50.0% |
| !! | GTTCTTGCACGGATTGGAAG+AGG | - | chr1.4:33060243-33060262 | None:intergenic | 50.0% |
| !! | TTCGATGGGTTGAAGGGAAG+TGG | - | chr1.4:33060188-33060207 | None:intergenic | 50.0% |
| !!! | GATGGTGGTGGTGGTTTTGT+TGG | - | chr1.4:33060142-33060161 | None:intergenic | 50.0% |
| AATTGGGAGGGAGAGGGAAG+AGG | + | chr1.4:33060764-33060783 | MS.gene43355:CDS | 55.0% | |
| AGTTTGGGTCCGAGCCTACT+TGG | + | chr1.4:33060366-33060385 | MS.gene43355:CDS | 55.0% | |
| GAGTAAAGCAGCGAGCCCTT+TGG | - | chr1.4:33060440-33060459 | None:intergenic | 55.0% | |
| ! | GCTGGTGTTAGAAGGAGCGA+TGG | + | chr1.4:33060865-33060884 | MS.gene43355:CDS | 55.0% |
| !! | ACCGACTTCAGAAGGCGTCT+TGG | - | chr1.4:33061022-33061041 | None:intergenic | 55.0% |
| !! | ACGCCTTCTGAAGTCGGTGA+AGG | + | chr1.4:33061024-33061043 | MS.gene43355:CDS | 55.0% |
| !! | GCCAAGACGCCTTCTGAAGT+CGG | + | chr1.4:33061018-33061037 | MS.gene43355:CDS | 55.0% |
| TGGGTCCGAGCCTACTTGGA+TGG | + | chr1.4:33060370-33060389 | MS.gene43355:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 33059989 | 33061050 | 33059989 | ID=MS.gene43355 |
| chr1.4 | mRNA | 33059989 | 33061050 | 33059989 | ID=MS.gene43355.t1;Parent=MS.gene43355 |
| chr1.4 | exon | 33059989 | 33061050 | 33059989 | ID=MS.gene43355.t1.exon1;Parent=MS.gene43355.t1 |
| chr1.4 | CDS | 33059989 | 33061050 | 33059989 | ID=cds.MS.gene43355.t1;Parent=MS.gene43355.t1 |
| Gene Sequence |
| Protein sequence |
