AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44231


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44231.t1 MTR_5g028350 85.813 289 38 2 1 286 1 289 1.11e-169 471
MS.gene44231.t1 MTR_8g075400 86.760 287 11 2 1 287 14 273 2.69e-162 452
MS.gene44231.t1 MTR_8g075400 97.748 222 5 0 1 222 1 222 2.53e-157 439
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44231.t1 AT3G22660 64.583 288 95 3 2 288 12 293 8.01e-126 360

Find 68 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCTCCAAAGCCAAATT+TGG 0.135328 8.1:-31742174 None:intergenic
GCTGATACTACTAATGATTT+TGG 0.192046 8.1:+31742221 MS.gene44231:CDS
GATGTTAAGAATTCCAAATT+TGG 0.278385 8.1:+31742161 MS.gene44231:CDS
ATTAGCTGAACCATCTAAAA+AGG 0.298870 8.1:+31741533 MS.gene44231:CDS
AGAAAGGGCCAAGCAGAAAA+AGG 0.337714 8.1:+31741935 MS.gene44231:CDS
AGTCTGCAGGCCTTAGAAAA+AGG 0.345729 8.1:-31741761 None:intergenic
TGTATCCATTCCACATTCTC+TGG 0.362791 8.1:-31741606 None:intergenic
AATTCCAAATTTGGCTTTGG+AGG 0.373337 8.1:+31742170 MS.gene44231:CDS
AAGAATTCCAAATTTGGCTT+TGG 0.378859 8.1:+31742167 MS.gene44231:CDS
CTGAAGAGAGAAGAAAGGCC+AGG 0.422247 8.1:+31741868 MS.gene44231:CDS
GAAAAGACCTGGAGTGTCTC+CGG 0.423295 8.1:+31742076 MS.gene44231:CDS
AGATAATCTCTTGGCCTCCC+TGG 0.430466 8.1:-31741886 None:intergenic
AGTCACAGAAATTAAAAGAA+AGG 0.442695 8.1:+31741919 MS.gene44231:CDS
GTGTCTCCGGGTGACCGATC+AGG 0.444973 8.1:+31742089 MS.gene44231:CDS
CTTTGAGAAACTTCAGTCAA+TGG 0.454306 8.1:+31741734 MS.gene44231:CDS
CGATGGCGCAGATAAGGCTT+TGG 0.456534 8.1:+31742016 MS.gene44231:CDS
TGCAGACTATTATGCTGAAA+TGG 0.457564 8.1:+31741776 MS.gene44231:CDS
CTGCTCTTGATCGATGTCAA+TGG 0.460523 8.1:-31741637 None:intergenic
GTCACAGAAATTAAAAGAAA+GGG 0.462057 8.1:+31741920 MS.gene44231:CDS
GGTAAGGCAAAGCAAGCCTT+TGG 0.462245 8.1:+31742113 MS.gene44231:CDS
TGGCTTCTTTCCCTTTCCAA+AGG 0.463981 8.1:-31742129 None:intergenic
TGCTGACGATGGCGCAGATA+AGG 0.473187 8.1:+31742010 MS.gene44231:CDS
TTTGAGAAACTTCAGTCAAT+GGG 0.475684 8.1:+31741735 MS.gene44231:CDS
ACATTGAATCTGTTAAGAAA+TGG 0.479309 8.1:+31741961 MS.gene44231:CDS
GAGGAAGTAAGAAGAGAAAG+AGG 0.489339 8.1:+31742270 MS.gene44231:CDS
GCAAAGCAAGCCTTTGGAAA+GGG 0.492131 8.1:+31742119 MS.gene44231:CDS
AATGATGATACAATGGTTGA+TGG 0.492459 8.1:+31741447 MS.gene44231:CDS
AACCTCTTTAGATAATCTCT+TGG 0.505477 8.1:-31741895 None:intergenic
TTACCTCCTGATCGGTCACC+CGG 0.505687 8.1:-31742095 None:intergenic
TTTGGCTTTGGAGGCAAGAA+GGG 0.507591 8.1:+31742179 MS.gene44231:CDS
CATAAGCTGGCCAGAGAATG+TGG 0.514747 8.1:+31741596 MS.gene44231:CDS
AGATAAGGCTTTGGACTTCG+AGG 0.515894 8.1:+31742025 MS.gene44231:CDS
AGATTGAATGGAGCTGCCTC+TGG 0.515931 8.1:+31741422 None:intergenic
GGCATTGGAGGGTACTAGAC+AGG 0.518118 8.1:+31741710 MS.gene44231:CDS
TCTTCTGATTCTGATTCAGA+TGG 0.519325 8.1:-31741501 None:intergenic
ATTTGGCTTTGGAGGCAAGA+AGG 0.519353 8.1:+31742178 MS.gene44231:CDS
GCTTTGCCTTACCTCCTGAT+CGG 0.526130 8.1:-31742103 None:intergenic
AGAGGCTGAAGAGAGAAGAA+AGG 0.526636 8.1:+31741863 MS.gene44231:CDS
GATGCTCTATTAGATAAACT+TGG 0.532375 8.1:+31741570 MS.gene44231:CDS
AAAAGACCTGGAGTGTCTCC+GGG 0.535827 8.1:+31742077 MS.gene44231:CDS
ATTAGCAGAGAAGCAGAAGA+TGG 0.537363 8.1:+31741839 MS.gene44231:CDS
CGGTCAAAGAAGAAAAGACC+TGG 0.554286 8.1:+31742065 MS.gene44231:CDS
ATTTCAGCATAATAGTCTGC+AGG 0.562441 8.1:-31741774 None:intergenic
TGAAGAGAGAAGAAAGGCCA+GGG 0.563172 8.1:+31741869 MS.gene44231:CDS
CATTGACATCGATCAAGAGC+AGG 0.572289 8.1:+31741638 MS.gene44231:CDS
ATAAACTTGGAGACATAAGC+TGG 0.573791 8.1:+31741583 MS.gene44231:CDS
GGGTGACCGATCAGGAGGTA+AGG 0.574615 8.1:+31742097 MS.gene44231:CDS
CGGTCACCCGGAGACACTCC+AGG 0.576723 8.1:-31742083 None:intergenic
GGCCAAGAGATTATCTAAAG+AGG 0.579968 8.1:+31741893 MS.gene44231:CDS
GGCAAAGCAAGCCTTTGGAA+AGG 0.581107 8.1:+31742118 MS.gene44231:CDS
AGAGAAGCAGAAGATGGTAG+AGG 0.581371 8.1:+31741845 MS.gene44231:CDS
AGTAGATGTTAATGATGACT+TGG 0.588825 8.1:+31741662 MS.gene44231:CDS
TTGAATCTGTTAAGAAATGG+AGG 0.588933 8.1:+31741964 MS.gene44231:CDS
TTTGGACTTCGAGGACGGAA+AGG 0.593158 8.1:+31742034 MS.gene44231:CDS
AGGAAGCAGAGGCAACAAAG+TGG 0.602796 8.1:+31741984 MS.gene44231:CDS
AAGGCTTTGGACTTCGAGGA+CGG 0.606211 8.1:+31742029 MS.gene44231:CDS
GGAAGCAGAGGCAACAAAGT+GGG 0.615514 8.1:+31741985 MS.gene44231:CDS
CAAAGTGGGTTTGCTGACGA+TGG 0.619387 8.1:+31741999 MS.gene44231:CDS
TTAAGAAATGGAGGAAGCAG+AGG 0.621715 8.1:+31741973 MS.gene44231:CDS
CATTGACTGAAGTTTCTCAA+AGG 0.625618 8.1:-31741733 None:intergenic
TCTCCGGGTGACCGATCAGG+AGG 0.644315 8.1:+31742092 MS.gene44231:CDS
GCTGGCCAGAGAATGTGGAA+TGG 0.647233 8.1:+31741601 MS.gene44231:CDS
AGAGAGAAGAAAGGCCAGGG+AGG 0.661477 8.1:+31741872 MS.gene44231:CDS
TCTGGTTAATGATGATACAA+TGG 0.663757 8.1:+31741440 MS.gene44231:CDS
ATTGTATCATCATTAACCAG+AGG 0.690136 8.1:-31741438 None:intergenic
TGAAGCTGAGTATTTGAGTG+AGG 0.691291 8.1:+31741470 MS.gene44231:CDS
AGGACGGAAAGGTGTTCGAG+CGG 0.718016 8.1:+31742045 MS.gene44231:CDS
GGTGAAGACAGATAGCCACA+TGG 0.738846 8.1:+31741797 MS.gene44231:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACATTGAATCTGTTAAGAAA+TGG + chr8.1:31741961-31741980 MS.gene44231:CDS 25.0%
! AGTCACAGAAATTAAAAGAA+AGG + chr8.1:31741919-31741938 MS.gene44231:CDS 25.0%
! GATGTTAAGAATTCCAAATT+TGG + chr8.1:31742161-31742180 MS.gene44231:CDS 25.0%
! GTCACAGAAATTAAAAGAAA+GGG + chr8.1:31741920-31741939 MS.gene44231:CDS 25.0%
!! TTATTTACTGCCTTTTTAGA+TGG - chr8.1:31741546-31741565 None:intergenic 25.0%
!! TTCTTAACATCTCTTTTCTT+TGG - chr8.1:31742152-31742171 None:intergenic 25.0%
AACCTCTTTAGATAATCTCT+TGG - chr8.1:31741898-31741917 None:intergenic 30.0%
AATGATGATACAATGGTTGA+TGG + chr8.1:31741447-31741466 MS.gene44231:CDS 30.0%
ACATGGAAAAAGTGAAAAGT+CGG + chr8.1:31741814-31741833 MS.gene44231:CDS 30.0%
AGTAGATGTTAATGATGACT+TGG + chr8.1:31741662-31741681 MS.gene44231:CDS 30.0%
ATTAGCTGAACCATCTAAAA+AGG + chr8.1:31741533-31741552 MS.gene44231:CDS 30.0%
ATTGTATCATCATTAACCAG+AGG - chr8.1:31741441-31741460 None:intergenic 30.0%
GATGCTCTATTAGATAAACT+TGG + chr8.1:31741570-31741589 MS.gene44231:CDS 30.0%
TCTGGTTAATGATGATACAA+TGG + chr8.1:31741440-31741459 MS.gene44231:CDS 30.0%
TTGAATCTGTTAAGAAATGG+AGG + chr8.1:31741964-31741983 MS.gene44231:CDS 30.0%
TTTGAGAAACTTCAGTCAAT+GGG + chr8.1:31741735-31741754 MS.gene44231:CDS 30.0%
! AAGAATTCCAAATTTGGCTT+TGG + chr8.1:31742167-31742186 MS.gene44231:CDS 30.0%
! GATACTACTAATGATTTTGG+TGG + chr8.1:31742224-31742243 MS.gene44231:CDS 30.0%
! GCTGATACTACTAATGATTT+TGG + chr8.1:31742221-31742240 MS.gene44231:CDS 30.0%
ATAAACTTGGAGACATAAGC+TGG + chr8.1:31741583-31741602 MS.gene44231:CDS 35.0%
ATTTCAGCATAATAGTCTGC+AGG - chr8.1:31741777-31741796 None:intergenic 35.0%
TCTTCTGATTCTGATTCAGA+TGG - chr8.1:31741504-31741523 None:intergenic 35.0%
TGCAGACTATTATGCTGAAA+TGG + chr8.1:31741776-31741795 MS.gene44231:CDS 35.0%
! CATTGACTGAAGTTTCTCAA+AGG - chr8.1:31741736-31741755 None:intergenic 35.0%
! CTTTGAGAAACTTCAGTCAA+TGG + chr8.1:31741734-31741753 MS.gene44231:CDS 35.0%
! TTTGGTGGCTTCAGTAAAAA+GGG + chr8.1:31742239-31742258 MS.gene44231:CDS 35.0%
!! AATTCCAAATTTGGCTTTGG+AGG + chr8.1:31742170-31742189 MS.gene44231:CDS 35.0%
!! TTTTGGTGGCTTCAGTAAAA+AGG + chr8.1:31742238-31742257 MS.gene44231:CDS 35.0%
!!! GACTTTTCACTTTTTCCATG+TGG - chr8.1:31741815-31741834 None:intergenic 35.0%
ATTAGCAGAGAAGCAGAAGA+TGG + chr8.1:31741839-31741858 MS.gene44231:CDS 40.0%
GAGGAAGTAAGAAGAGAAAG+AGG + chr8.1:31742270-31742289 MS.gene44231:CDS 40.0%
GGCCAAGAGATTATCTAAAG+AGG + chr8.1:31741893-31741912 MS.gene44231:CDS 40.0%
TGAAGCTGAGTATTTGAGTG+AGG + chr8.1:31741470-31741489 MS.gene44231:CDS 40.0%
TGTATCCATTCCACATTCTC+TGG - chr8.1:31741609-31741628 None:intergenic 40.0%
TTAAGAAATGGAGGAAGCAG+AGG + chr8.1:31741973-31741992 MS.gene44231:CDS 40.0%
! AATGTCGTCCTTTTTCTGCT+TGG - chr8.1:31741946-31741965 None:intergenic 40.0%
! AGAGCTTGCATTTTACACTC+AGG + chr8.1:31741689-31741708 MS.gene44231:CDS 40.0%
! CAATGGGTCTCCTTTTTCTA+AGG + chr8.1:31741751-31741770 MS.gene44231:CDS 40.0%
! TGCATTTTACACTCAGGCAT+TGG + chr8.1:31741695-31741714 MS.gene44231:CDS 40.0%
!! TTCAGTAAAAAGGGTGCTGT+TGG + chr8.1:31742248-31742267 MS.gene44231:CDS 40.0%
!!! ATTTTACACTCAGGCATTGG+AGG + chr8.1:31741698-31741717 MS.gene44231:CDS 40.0%
!!! TTTTACACTCAGGCATTGGA+GGG + chr8.1:31741699-31741718 MS.gene44231:CDS 40.0%
AGAAAGGGCCAAGCAGAAAA+AGG + chr8.1:31741935-31741954 MS.gene44231:CDS 45.0%
AGAGAAGCAGAAGATGGTAG+AGG + chr8.1:31741845-31741864 MS.gene44231:CDS 45.0%
AGAGGCTGAAGAGAGAAGAA+AGG + chr8.1:31741863-31741882 MS.gene44231:CDS 45.0%
AGTCTGCAGGCCTTAGAAAA+AGG - chr8.1:31741764-31741783 None:intergenic 45.0%
CATTGACATCGATCAAGAGC+AGG + chr8.1:31741638-31741657 MS.gene44231:CDS 45.0%
CGGTCAAAGAAGAAAAGACC+TGG + chr8.1:31742065-31742084 MS.gene44231:CDS 45.0%
CTGCTCTTGATCGATGTCAA+TGG - chr8.1:31741640-31741659 None:intergenic 45.0%
CTTGCCTCCAAAGCCAAATT+TGG - chr8.1:31742177-31742196 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGCCA+GGG + chr8.1:31741869-31741888 MS.gene44231:CDS 45.0%
TGGCTTCTTTCCCTTTCCAA+AGG - chr8.1:31742132-31742151 None:intergenic 45.0%
! GCAAAGCAAGCCTTTGGAAA+GGG + chr8.1:31742119-31742138 MS.gene44231:CDS 45.0%
!! AGATAAGGCTTTGGACTTCG+AGG + chr8.1:31742025-31742044 MS.gene44231:CDS 45.0%
!! AGTAAAAAGGGTGCTGTTGG+AGG + chr8.1:31742251-31742270 MS.gene44231:CDS 45.0%
!! ATTTGGCTTTGGAGGCAAGA+AGG + chr8.1:31742178-31742197 MS.gene44231:CDS 45.0%
!! TTTGGCTTTGGAGGCAAGAA+GGG + chr8.1:31742179-31742198 MS.gene44231:CDS 45.0%
AGATAATCTCTTGGCCTCCC+TGG - chr8.1:31741889-31741908 None:intergenic 50.0%
AGGAAGCAGAGGCAACAAAG+TGG + chr8.1:31741984-31742003 MS.gene44231:CDS 50.0%
CATAAGCTGGCCAGAGAATG+TGG + chr8.1:31741596-31741615 MS.gene44231:CDS 50.0%
CTGAAGAGAGAAGAAAGGCC+AGG + chr8.1:31741868-31741887 MS.gene44231:CDS 50.0%
GCTTTGCCTTACCTCCTGAT+CGG - chr8.1:31742106-31742125 None:intergenic 50.0%
GGAAGCAGAGGCAACAAAGT+GGG + chr8.1:31741985-31742004 MS.gene44231:CDS 50.0%
GGTAAGGCAAAGCAAGCCTT+TGG + chr8.1:31742113-31742132 MS.gene44231:CDS 50.0%
GGTGAAGACAGATAGCCACA+TGG + chr8.1:31741797-31741816 MS.gene44231:CDS 50.0%
TTTGGACTTCGAGGACGGAA+AGG + chr8.1:31742034-31742053 MS.gene44231:CDS 50.0%
! AAAAGACCTGGAGTGTCTCC+GGG + chr8.1:31742077-31742096 MS.gene44231:CDS 50.0%
! GAAAAGACCTGGAGTGTCTC+CGG + chr8.1:31742076-31742095 MS.gene44231:CDS 50.0%
! GGCAAAGCAAGCCTTTGGAA+AGG + chr8.1:31742118-31742137 MS.gene44231:CDS 50.0%
!! AAGGCTTTGGACTTCGAGGA+CGG + chr8.1:31742029-31742048 MS.gene44231:CDS 50.0%
!! CAAAGTGGGTTTGCTGACGA+TGG + chr8.1:31741999-31742018 MS.gene44231:CDS 50.0%
AGAGAGAAGAAAGGCCAGGG+AGG + chr8.1:31741872-31741891 MS.gene44231:CDS 55.0%
CGATGGCGCAGATAAGGCTT+TGG + chr8.1:31742016-31742035 MS.gene44231:CDS 55.0%
GCTGGCCAGAGAATGTGGAA+TGG + chr8.1:31741601-31741620 MS.gene44231:CDS 55.0%
TGCTGACGATGGCGCAGATA+AGG + chr8.1:31742010-31742029 MS.gene44231:CDS 55.0%
TTACCTCCTGATCGGTCACC+CGG - chr8.1:31742098-31742117 None:intergenic 55.0%
!! AGGACGGAAAGGTGTTCGAG+CGG + chr8.1:31742045-31742064 MS.gene44231:CDS 55.0%
!! GGCATTGGAGGGTACTAGAC+AGG + chr8.1:31741710-31741729 MS.gene44231:CDS 55.0%
GGGTGACCGATCAGGAGGTA+AGG + chr8.1:31742097-31742116 MS.gene44231:CDS 60.0%
GTGTCTCCGGGTGACCGATC+AGG + chr8.1:31742089-31742108 MS.gene44231:CDS 65.0%
TCTCCGGGTGACCGATCAGG+AGG + chr8.1:31742092-31742111 MS.gene44231:CDS 65.0%
CGGTCACCCGGAGACACTCC+AGG - chr8.1:31742086-31742105 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31741429 31742295 31741429 ID=MS.gene44231
chr8.1 mRNA 31741429 31742295 31741429 ID=MS.gene44231.t1;Parent=MS.gene44231
chr8.1 exon 31741429 31742295 31741429 ID=MS.gene44231.t1.exon1;Parent=MS.gene44231.t1
chr8.1 CDS 31741429 31742295 31741429 ID=cds.MS.gene44231.t1;Parent=MS.gene44231.t1
Gene Sequence

>MS.gene44231

ATGGAGCTGCCTCTGGTTAATGATGATACAATGGTTGATGGTGAAGCTGAGTATTTGAGTGAGGAAATGTCACCATCTGAATCAGAATCAGAAGAAGATGTGAAATTAGCTGAACCATCTAAAAAGGCAGTAAATAACAGAGATGCTCTATTAGATAAACTTGGAGACATAAGCTGGCCAGAGAATGTGGAATGGATACACAAACTCTCCATTGACATCGATCAAGAGCAGGAAGTAGATGTTAATGATGACTTGGCACGAGAGCTTGCATTTTACACTCAGGCATTGGAGGGTACTAGACAGGCCTTTGAGAAACTTCAGTCAATGGGTCTCCTTTTTCTAAGGCCTGCAGACTATTATGCTGAAATGGTGAAGACAGATAGCCACATGGAAAAAGTGAAAAGTCGGCTATTAGCAGAGAAGCAGAAGATGGTAGAGGCTGAAGAGAGAAGAAAGGCCAGGGAGGCCAAGAGATTATCTAAAGAGGTTCAGTCACAGAAATTAAAAGAAAGGGCCAAGCAGAAAAAGGACGACATTGAATCTGTTAAGAAATGGAGGAAGCAGAGGCAACAAAGTGGGTTTGCTGACGATGGCGCAGATAAGGCTTTGGACTTCGAGGACGGAAAGGTGTTCGAGCGGTCAAAGAAGAAAAGACCTGGAGTGTCTCCGGGTGACCGATCAGGAGGTAAGGCAAAGCAAGCCTTTGGAAAGGGAAAGAAGCCAAAGAAAAGAGATGTTAAGAATTCCAAATTTGGCTTTGGAGGCAAGAAGGGTTTGAAGAAGCAGAATACAGCTGATACTACTAATGATTTTGGTGGCTTCAGTAAAAAGGGTGCTGTTGGAGGAAGTAAGAAGAGAAAGAGGTAA

Protein sequence

>MS.gene44231.t1

MELPLVNDDTMVDGEAEYLSEEMSPSESESEEDVKLAEPSKKAVNNRDALLDKLGDISWPENVEWIHKLSIDIDQEQEVDVNDDLARELAFYTQALEGTRQAFEKLQSMGLLFLRPADYYAEMVKTDSHMEKVKSRLLAEKQKMVEAEERRKAREAKRLSKEVQSQKLKERAKQKKDDIESVKKWRKQRQQSGFADDGADKALDFEDGKVFERSKKKRPGVSPGDRSGGKAKQAFGKGKKPKKRDVKNSKFGFGGKKGLKKQNTADTTNDFGGFSKKGAVGGSKKRKR