AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47835


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47835.t1 MTR_4g015540 95.122 123 6 0 1 123 1 123 6.14e-71 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47835.t1 AT2G38140 49.580 119 58 1 5 123 2 118 4.06e-26 94.7

Find 39 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCAAGCTTCTCTTTCT+TGG 0.182199 4.4:+84933019 None:intergenic
TCGCTTTCACCAACCCCTTC+TGG 0.306084 4.4:-84933901 MS.gene47835:intron
GTACCCTTGTTCTTGTTCCT+TGG 0.342529 4.4:+84933077 None:intergenic
TTCTCTTTCTTGGTAGCATC+TGG 0.349140 4.4:+84933029 None:intergenic
GAAAGAGAAGCTTGAAGATA+AGG 0.394365 4.4:-84933015 MS.gene47835:CDS
AAACGGTTCAGCCATTCGTT+TGG 0.400874 4.4:-84933732 MS.gene47835:CDS
TGTATTGTGGCAGAGGTGAT+AGG 0.424452 4.4:-84933770 MS.gene47835:CDS
GCGTGGAGTTGAAGAGAGAG+TGG 0.494775 4.4:+84934012 None:intergenic
TCTTTCTTGGTAGCATCTGG+TGG 0.499433 4.4:+84933032 None:intergenic
AAAGGTGAGAAGAGGAAGCG+TGG 0.518100 4.4:+84933995 None:intergenic
ATTGGGATTGAGAGAAGGAA+AGG 0.519722 4.4:+84933977 None:intergenic
AGAGAAGGAAAGGTGAGAAG+AGG 0.519757 4.4:+84933987 None:intergenic
GGAAAACTGCTAAGGGGAAA+CGG 0.521583 4.4:-84933749 MS.gene47835:CDS
GATTGGTAAGTGAAGAGATT+GGG 0.527445 4.4:+84933960 None:intergenic
AAGAGATTGGGATTGAGAGA+AGG 0.529493 4.4:+84933972 None:intergenic
AAATCACCTAACTAGAAAAG+AGG 0.532100 4.4:+84932964 None:intergenic
AGGAACAAGAACAAGGGTAC+AGG 0.550303 4.4:-84933074 MS.gene47835:CDS
TGATTGGTAAGTGAAGAGAT+TGG 0.551437 4.4:+84933959 None:intergenic
AGGTGATAGGAAAACTGCTA+AGG 0.552164 4.4:-84933757 MS.gene47835:CDS
TCTGGTGGAGCATAAATCCT+TGG 0.553001 4.4:+84933047 None:intergenic
GGGACTGATGAAGTGATGAT+TGG 0.562516 4.4:+84933943 None:intergenic
AGTTGAAGAGAGAGTGGTGA+AGG 0.566595 4.4:+84934018 None:intergenic
GGTGATAGGAAAACTGCTAA+GGG 0.567207 4.4:-84933756 MS.gene47835:CDS
ACATTACATTTCCAAACGAA+TGG 0.569487 4.4:+84933721 None:intergenic
ACAAGGTTAAAGAGAGGGTG+GGG 0.576219 4.4:+84933923 None:intergenic
GGTGGAGCATAAATCCTTGG+TGG 0.582114 4.4:+84933050 None:intergenic
AATCACCTAACTAGAAAAGA+GGG 0.586436 4.4:+84932965 None:intergenic
AAGGCCAAGGAACAAGAACA+AGG 0.590824 4.4:-84933081 MS.gene47835:CDS
TATCCTCTCAGTTATGGCCA+TGG 0.592654 4.4:-84934064 None:intergenic
CGACAAGGTTAAAGAGAGGG+TGG 0.596467 4.4:+84933921 None:intergenic
GACAAGGTTAAAGAGAGGGT+GGG 0.598803 4.4:+84933922 None:intergenic
AATGTGATCAGGCAAGGCCA+AGG 0.609619 4.4:-84933094 MS.gene47835:intron
TAGAAGCAGTGATGATGCCA+TGG 0.657767 4.4:+84934047 None:intergenic
AAGCGACAAGGTTAAAGAGA+GGG 0.666570 4.4:+84933918 None:intergenic
AGGGGTTGGTGAAAGCGACA+AGG 0.670121 4.4:+84933906 None:intergenic
AAAGCGACAAGGTTAAAGAG+AGG 0.678658 4.4:+84933917 None:intergenic
AGGCCAAGGAACAAGAACAA+GGG 0.711024 4.4:-84933080 MS.gene47835:CDS
GTGATAGGAAAACTGCTAAG+GGG 0.715035 4.4:-84933755 MS.gene47835:CDS
ACAAGGGTACAGGACCACCA+AGG 0.743357 4.4:-84933064 MS.gene47835:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATCATTATTATTGTT+TGG - chr4.4:84933823-84933842 MS.gene47835:intron 10.0%
!!! TTTTGCTAAATTATTATAAA+AGG - chr4.4:84933512-84933531 MS.gene47835:intron 10.0%
!! AATAATTTAGCAAAACAAAA+AGG + chr4.4:84933507-84933526 None:intergenic 15.0%
!! GAAATTCAAAATGTATAAAA+TGG + chr4.4:84933336-84933355 None:intergenic 15.0%
!! AAAAATTCGAGATTATTTCA+TGG - chr4.4:84933789-84933808 MS.gene47835:intron 20.0%
!! AACGAACCAAAATAAAAAAA+TGG + chr4.4:84933216-84933235 None:intergenic 20.0%
!! ACGAACCAAAATAAAAAAAT+GGG + chr4.4:84933215-84933234 None:intergenic 20.0%
!! TTCAAATTAACATTAGAACT+AGG + chr4.4:84933889-84933908 None:intergenic 20.0%
!!! AACAAAAATTCCATTTTTCT+TGG + chr4.4:84933158-84933177 None:intergenic 20.0%
!!! AATTTTTTCTCCAAGAAAAA+TGG - chr4.4:84933145-84933164 MS.gene47835:intron 20.0%
!!! GATAACCCATTTTTTTATTT+TGG - chr4.4:84933207-84933226 MS.gene47835:intron 20.0%
!!! TGGAATTTTTGTTATGAATT+TGG - chr4.4:84933165-84933184 MS.gene47835:intron 20.0%
! ATACACTAACAGTGTAAAAT+AGG + chr4.4:84933651-84933670 None:intergenic 25.0%
! CTACAAATTAGAAAACTACA+CGG + chr4.4:84933740-84933759 None:intergenic 25.0%
! GAATTTATTGAGTGTATGTT+TGG - chr4.4:84933365-84933384 MS.gene47835:intron 25.0%
!! TGTTTTCTTTTAGTGTATTG+TGG - chr4.4:84933239-84933258 MS.gene47835:intron 25.0%
!! TTGTCATTTAAAATGTGATC+AGG - chr4.4:84933917-84933936 MS.gene47835:CDS 25.0%
ACATTACATTTCCAAACGAA+TGG + chr4.4:84933304-84933323 None:intergenic 30.0%
ATCCATTTAACTCATGTTTC+TGG - chr4.4:84933671-84933690 MS.gene47835:intron 30.0%
TATTGAGTGTATGTTTGGTT+AGG - chr4.4:84933370-84933389 MS.gene47835:intron 30.0%
TGGAGAAAAAATTACCAGAA+GGG + chr4.4:84933138-84933157 None:intergenic 30.0%
TTCAAAAGTGGTGATAAAAC+AGG - chr4.4:84933593-84933612 MS.gene47835:intron 30.0%
TTGGAGAAAAAATTACCAGA+AGG + chr4.4:84933139-84933158 None:intergenic 30.0%
! ATTTAAAATGTGATCAGGCA+AGG - chr4.4:84933922-84933941 MS.gene47835:CDS 30.0%
! TTACATCTGCATAAAGCATT+TGG - chr4.4:84933561-84933580 MS.gene47835:intron 30.0%
!! AATGTACGTTGATTCATGTT+TGG - chr4.4:84933447-84933466 MS.gene47835:intron 30.0%
!! CGTGTAGTTTTCTAATTTGT+AGG - chr4.4:84933738-84933757 MS.gene47835:CDS 30.0%
!!! GATTTTTTAGTCCATGAAGA+GGG - chr4.4:84933707-84933726 MS.gene47835:intron 30.0%
!!! TGATTTTTTAGTCCATGAAG+AGG - chr4.4:84933706-84933725 MS.gene47835:intron 30.0%
!!! TGGTGGTTATTTTTCAAAAG+TGG - chr4.4:84933581-84933600 MS.gene47835:intron 30.0%
AAAAAATTACCAGAAGGGGT+TGG + chr4.4:84933133-84933152 None:intergenic 35.0%
ATCTTCAAGCTTCTCTTTCT+TGG + chr4.4:84934006-84934025 None:intergenic 35.0%
GAAAGAGAAGCTTGAAGATA+AGG - chr4.4:84934007-84934026 MS.gene47835:CDS 35.0%
GACCAGAAACATGAGTTAAA+TGG + chr4.4:84933676-84933695 None:intergenic 35.0%
GATTGGTAAGTGAAGAGATT+GGG + chr4.4:84933065-84933084 None:intergenic 35.0%
GGACTGAAAAGTAAATACTG+AGG - chr4.4:84933759-84933778 MS.gene47835:CDS 35.0%
GGAGAAAAAATTACCAGAAG+GGG + chr4.4:84933137-84933156 None:intergenic 35.0%
TGATTGGTAAGTGAAGAGAT+TGG + chr4.4:84933066-84933085 None:intergenic 35.0%
AAAGCGACAAGGTTAAAGAG+AGG + chr4.4:84933108-84933127 None:intergenic 40.0%
AAGAGATTGGGATTGAGAGA+AGG + chr4.4:84933053-84933072 None:intergenic 40.0%
AAGCGACAAGGTTAAAGAGA+GGG + chr4.4:84933107-84933126 None:intergenic 40.0%
AGGTGATAGGAAAACTGCTA+AGG - chr4.4:84933265-84933284 MS.gene47835:intron 40.0%
ATTGGGATTGAGAGAAGGAA+AGG + chr4.4:84933048-84933067 None:intergenic 40.0%
GGTGATAAAACAGGCTTACA+TGG - chr4.4:84933602-84933621 MS.gene47835:intron 40.0%
GGTGATAGGAAAACTGCTAA+GGG - chr4.4:84933266-84933285 MS.gene47835:intron 40.0%
GTGATAGGAAAACTGCTAAG+GGG - chr4.4:84933267-84933286 MS.gene47835:intron 40.0%
! CATCTGCATAAAGCATTTGG+TGG - chr4.4:84933564-84933583 MS.gene47835:intron 40.0%
! CTTTTAGTGTATTGTGGCAG+AGG - chr4.4:84933245-84933264 MS.gene47835:intron 40.0%
! TTCTCTTTCTTGGTAGCATC+TGG + chr4.4:84933996-84934015 None:intergenic 40.0%
AAACGGTTCAGCCATTCGTT+TGG - chr4.4:84933290-84933309 MS.gene47835:intron 45.0%
AAGGCCAAGGAACAAGAACA+AGG - chr4.4:84933941-84933960 MS.gene47835:CDS 45.0%
ACAAGGTTAAAGAGAGGGTG+GGG + chr4.4:84933102-84933121 None:intergenic 45.0%
ACGGTTTAAGTCCCTCTTCA+TGG + chr4.4:84933721-84933740 None:intergenic 45.0%
AGAGAAGGAAAGGTGAGAAG+AGG + chr4.4:84933038-84933057 None:intergenic 45.0%
AGGCCAAGGAACAAGAACAA+GGG - chr4.4:84933942-84933961 MS.gene47835:CDS 45.0%
AGTTGAAGAGAGAGTGGTGA+AGG + chr4.4:84933007-84933026 None:intergenic 45.0%
GACAAGGTTAAAGAGAGGGT+GGG + chr4.4:84933103-84933122 None:intergenic 45.0%
GGAAAACTGCTAAGGGGAAA+CGG - chr4.4:84933273-84933292 MS.gene47835:intron 45.0%
GGGACTGATGAAGTGATGAT+TGG + chr4.4:84933082-84933101 None:intergenic 45.0%
GTACCCTTGTTCTTGTTCCT+TGG + chr4.4:84933948-84933967 None:intergenic 45.0%
TCTGGTGGAGCATAAATCCT+TGG + chr4.4:84933978-84933997 None:intergenic 45.0%
TGTATTGTGGCAGAGGTGAT+AGG - chr4.4:84933252-84933271 MS.gene47835:intron 45.0%
! AGGAACAAGAACAAGGGTAC+AGG - chr4.4:84933948-84933967 MS.gene47835:CDS 45.0%
! TAGAAGCAGTGATGATGCCA+TGG + chr4.4:84932978-84932997 None:intergenic 45.0%
! TCTTTCTTGGTAGCATCTGG+TGG + chr4.4:84933993-84934012 None:intergenic 45.0%
AAAGGTGAGAAGAGGAAGCG+TGG + chr4.4:84933030-84933049 None:intergenic 50.0%
AATGTGATCAGGCAAGGCCA+AGG - chr4.4:84933928-84933947 MS.gene47835:CDS 50.0%
CGACAAGGTTAAAGAGAGGG+TGG + chr4.4:84933104-84933123 None:intergenic 50.0%
GGTGGAGCATAAATCCTTGG+TGG + chr4.4:84933975-84933994 None:intergenic 50.0%
GCGTGGAGTTGAAGAGAGAG+TGG + chr4.4:84933013-84933032 None:intergenic 55.0%
TCGCTTTCACCAACCCCTTC+TGG - chr4.4:84933121-84933140 MS.gene47835:intron 55.0%
! ACAAGGGTACAGGACCACCA+AGG - chr4.4:84933958-84933977 MS.gene47835:CDS 55.0%
!! AGGGGTTGGTGAAAGCGACA+AGG + chr4.4:84933119-84933138 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 84932971 84934073 84932971 ID=MS.gene47835
chr4.4 mRNA 84932971 84934073 84932971 ID=MS.gene47835.t1;Parent=MS.gene47835
chr4.4 exon 84933902 84934073 84933902 ID=MS.gene47835.t1.exon1;Parent=MS.gene47835.t1
chr4.4 CDS 84933902 84934073 84933902 ID=cds.MS.gene47835.t1;Parent=MS.gene47835.t1
chr4.4 exon 84933728 84933792 84933728 ID=MS.gene47835.t1.exon2;Parent=MS.gene47835.t1
chr4.4 CDS 84933728 84933792 84933728 ID=cds.MS.gene47835.t1;Parent=MS.gene47835.t1
chr4.4 exon 84932971 84933105 84932971 ID=MS.gene47835.t1.exon3;Parent=MS.gene47835.t1
chr4.4 CDS 84932971 84933105 84932971 ID=cds.MS.gene47835.t1;Parent=MS.gene47835.t1
Gene Sequence

>MS.gene47835

ATGGCCATGGCATCATCACTGCTTCTAGCATCACCTTCACCACTCTCTCTTCAACTCCACGCTTCCTCTTCTCACCTTTCCTTCTCTCAATCCCAATCTCTTCACTTACCAATCATCACTTCATCAGTCCCCACCCTCTCTTTAACCTTGTCGCTTTCACCAACCCCTTCTGGTAATTTTTTCTCCAAGAAAAATGGAATTTTTGTTATGAATTTGGTTTGTTGTGTTTGAATTTTGATAACCCATTTTTTTATTTTGGTTCGTTTTTTGTTTTCTTTTAGTGTATTGTGGCAGAGGTGATAGGAAAACTGCTAAGGGGAAACGGTTCAGCCATTCGTTTGGAAATGTAATGTTCTCTTTTTCCATTTTATACATTTTGAATTTCAAATTTATTGAATTTATTGAGTGTATGTTTGGTTAGGCATTGACGACGAGAATTGATTTTGATAAAGTTGATTTTAGTTAAAAGTGATTTGAATGTACGTTGATTCATGTTTGGATAAGTGATTCTTATTAATTCATAAATAATGTATCCTTTTTGTTTTGCTAAATTATTATAAAAGGATATGCATCGTCAGTGTAAACTAGTTTTACATCTGCATAAAGCATTTGGTGGTTATTTTTCAAAAGTGGTGATAAAACAGGCTTACATGGCAATATGATGATTTCTTATTAGACCTATTTTACACTGTTAGTGTATATCCATTTAACTCATGTTTCTGGTCTCTATATTACTGATTTTTTAGTCCATGAAGAGGGACTTAAACCGTGTAGTTTTCTAATTTGTAGGACTGAAAAGTAAATACTGAGGATAAAAAAAAAATTCGAGATTATTTCATGGACTAAAAACATATTTAATCATTATTATTGTTTGGTGATGATATTTGTTGAAATTGTTGTAGATGTAGTGTTTTTCCTAGTTCTAATGTTAATTTGAAAAAGAAATTTGTCATTTAAAATGTGATCAGGCAAGGCCAAGGAACAAGAACAAGGGTACAGGACCACCAAGGATTTATGCTCCACCAGATGCTACCAAGAAAGAGAAGCTTGAAGATAAGGAAGTGATTAAGATTGAAATTAATGAATCCCTCTTTTCTAGTTAG

Protein sequence

>MS.gene47835.t1

MAMASSLLLASPSPLSLQLHASSSHLSFSQSQSLHLPIITSSVPTLSLTLSLSPTPSVYCGRGDRKTAKGKRFSHSFGNARPRNKNKGTGPPRIYAPPDATKKEKLEDKEVIKIEINESLFSS