Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48989.t1 | XP_003605125.2 | 98.3 | 232 | 4 | 0 | 1 | 232 | 1 | 232 | 3.80E-124 | 454.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48989.t1 | G7JEM0 | 98.3 | 232 | 4 | 0 | 1 | 232 | 1 | 232 | 2.7e-124 | 454.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene48989 | -0.8088 | 2.59E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene48989 | -0.847498 | 1.11E-59 | -1.69E-46 |
| MS.gene049222 | MS.gene48989 | -0.83957 | 1.46E-57 | -1.69E-46 |
| MS.gene049232 | MS.gene48989 | -0.801657 | 8.12E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene48989 | -0.826655 | 2.41E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene48989 | -0.83208 | 1.16E-55 | -1.69E-46 |
| MS.gene049369 | MS.gene48989 | -0.820196 | 7.79E-53 | -1.69E-46 |
| MS.gene049549 | MS.gene48989 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene049609 | MS.gene48989 | -0.818083 | 2.36E-52 | -1.69E-46 |
| MS.gene049611 | MS.gene48989 | 0.810846 | 9.41E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene48989 | -0.821799 | 3.33E-53 | -1.69E-46 |
| MS.gene049634 | MS.gene48989 | 0.832148 | 1.12E-55 | -1.69E-46 |
| MS.gene050095 | MS.gene48989 | 0.811092 | 8.32E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene48989 | -0.807702 | 4.44E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene48989 | -0.844829 | 5.93E-59 | -1.69E-46 |
| MS.gene050165 | MS.gene48989 | -0.848208 | 7.10E-60 | -1.69E-46 |
| MS.gene050333 | MS.gene48989 | -0.829958 | 3.85E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene48989 | -0.839153 | 1.88E-57 | -1.69E-46 |
| MS.gene050518 | MS.gene48989 | -0.816734 | 4.75E-52 | -1.69E-46 |
| MS.gene050538 | MS.gene48989 | -0.82033 | 7.26E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene48989 | -0.801443 | 8.98E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene48989 | -0.836946 | 6.95E-57 | -1.69E-46 |
| MS.gene050647 | MS.gene48989 | 0.818193 | 2.23E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene48989 | -0.843434 | 1.40E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene48989 | -0.806852 | 6.73E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene48989 | -0.803344 | 3.65E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene48989 | -0.837153 | 6.15E-57 | -1.69E-46 |
| MS.gene05079 | MS.gene48989 | 0.818959 | 1.49E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene48989 | -0.811702 | 6.13E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene48989 | -0.81165 | 6.30E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene48989 | -0.809835 | 1.55E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene48989 | -0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene48989 | -0.82958 | 4.75E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene48989 | 0.819597 | 1.07E-52 | -1.69E-46 |
| MS.gene05114 | MS.gene48989 | 0.82207 | 2.88E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene48989 | -0.818968 | 1.49E-52 | -1.69E-46 |
| MS.gene051317 | MS.gene48989 | -0.81098 | 8.80E-51 | -1.69E-46 |
| MS.gene051617 | MS.gene48989 | -0.826067 | 3.32E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene48989 | -0.845419 | 4.11E-59 | -1.69E-46 |
| MS.gene051858 | MS.gene48989 | -0.828399 | 9.19E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene48989 | -0.803195 | 3.92E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene48989 | 0.823914 | 1.07E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene48989 | -0.818984 | 1.47E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene48989 | -0.836301 | 1.01E-56 | -1.69E-46 |
| MS.gene05244 | MS.gene48989 | 0.801653 | 8.13E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene48989 | -0.801416 | 9.10E-49 | -1.69E-46 |
| MS.gene052805 | MS.gene48989 | 0.808412 | 3.14E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene48989 | -0.808449 | 3.08E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene48989 | -0.808328 | 3.27E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene48989 | -0.823754 | 1.17E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene48989 | -0.812746 | 3.63E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene48989 | -0.809621 | 1.73E-50 | -1.69E-46 |
| MS.gene053213 | MS.gene48989 | -0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene48989 | -0.822889 | 1.86E-53 | -1.69E-46 |
| MS.gene053406 | MS.gene48989 | 0.80016 | 1.64E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene48989 | 0.835373 | 1.74E-56 | -1.69E-46 |
| MS.gene053561 | MS.gene48989 | 0.806201 | 9.23E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene48989 | -0.825849 | 3.75E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene48989 | -0.812903 | 3.35E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene48989 | -0.807672 | 4.51E-50 | -1.69E-46 |
| MS.gene053916 | MS.gene48989 | -0.807985 | 3.87E-50 | -1.69E-46 |
| MS.gene054421 | MS.gene48989 | 0.80614 | 9.51E-50 | -1.69E-46 |
| MS.gene054595 | MS.gene48989 | -0.81508 | 1.11E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene48989 | -0.817066 | 4.00E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene48989 | -0.825905 | 3.63E-54 | -1.69E-46 |
| MS.gene054706 | MS.gene48989 | 0.827415 | 1.58E-54 | -1.69E-46 |
| MS.gene05494 | MS.gene48989 | -0.800295 | 1.54E-48 | -1.69E-46 |
| MS.gene054954 | MS.gene48989 | -0.809974 | 1.45E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene48989 | 0.823479 | 1.35E-53 | -1.69E-46 |
| MS.gene055202 | MS.gene48989 | 0.811272 | 7.61E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene48989 | -0.809033 | 2.31E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene48989 | -0.807808 | 4.22E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene48989 | -0.82396 | 1.05E-53 | -1.69E-46 |
| MS.gene055397 | MS.gene48989 | -0.804312 | 2.29E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene48989 | -0.820418 | 6.93E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene48989 | -0.837828 | 4.13E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene48989 | -0.826932 | 2.07E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene48989 | -0.805413 | 1.35E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene48989 | -0.803413 | 3.53E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene48989 | -0.806764 | 7.02E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene48989 | -0.830214 | 3.33E-55 | -1.69E-46 |
| MS.gene055941 | MS.gene48989 | -0.822645 | 2.12E-53 | -1.69E-46 |
| MS.gene056487 | MS.gene48989 | -0.800553 | 1.37E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene48989 | -0.815536 | 8.79E-52 | -1.69E-46 |
| MS.gene056549 | MS.gene48989 | -0.815327 | 9.78E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene48989 | -0.841667 | 4.14E-58 | -1.69E-46 |
| MS.gene057076 | MS.gene48989 | -0.804879 | 1.75E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene48989 | -0.819791 | 9.65E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene48989 | -0.827593 | 1.44E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene48989 | -0.808638 | 2.81E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene48989 | -0.819306 | 1.24E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene48989 | -0.819407 | 1.18E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene48989 | -0.820775 | 5.74E-53 | -1.69E-46 |
| MS.gene058459 | MS.gene48989 | -0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene058588 | MS.gene48989 | -0.805806 | 1.12E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene48989 | 0.800126 | 1.67E-48 | -1.69E-46 |
| MS.gene058723 | MS.gene48989 | -0.829116 | 6.16E-55 | -1.69E-46 |
| MS.gene058773 | MS.gene48989 | -0.849178 | 3.82E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene48989 | -0.802963 | 4.37E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene48989 | 0.829567 | 4.79E-55 | -1.69E-46 |
| MS.gene058978 | MS.gene48989 | 0.814103 | 1.83E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene48989 | -0.83377 | 4.41E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene48989 | -0.816488 | 5.39E-52 | -1.69E-46 |
| MS.gene059029 | MS.gene48989 | -0.808505 | 3.00E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene48989 | -0.818774 | 1.65E-52 | -1.69E-46 |
| MS.gene059305 | MS.gene48989 | -0.811411 | 7.09E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene48989 | -0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene48989 | -0.801038 | 1.09E-48 | -1.69E-46 |
| MS.gene059677 | MS.gene48989 | -0.847155 | 1.38E-59 | -1.69E-46 |
| MS.gene059718 | MS.gene48989 | -0.819737 | 9.92E-53 | -1.69E-46 |
| MS.gene059865 | MS.gene48989 | -0.828927 | 6.85E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene48989 | -0.821944 | 3.08E-53 | -1.69E-46 |
| MS.gene059955 | MS.gene48989 | 0.808068 | 3.71E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene48989 | -0.806898 | 6.58E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene48989 | -0.833613 | 4.82E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene48989 | -0.811099 | 8.29E-51 | -1.69E-46 |
| MS.gene060818 | MS.gene48989 | -0.82126 | 4.44E-53 | -1.69E-46 |
| MS.gene060835 | MS.gene48989 | -0.802607 | 5.18E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene48989 | -0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene48989 | -0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene48989 | -0.810612 | 1.06E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene48989 | -0.826447 | 2.70E-54 | -1.69E-46 |
| MS.gene061150 | MS.gene48989 | -0.800589 | 1.34E-48 | -1.69E-46 |
| MS.gene061200 | MS.gene48989 | -0.822357 | 2.47E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48989.t1 | MTR_4g024370 | 98.276 | 232 | 4 | 0 | 1 | 232 | 1 | 232 | 4.87e-170 | 467 |
| MS.gene48989.t1 | MTR_4g024370 | 98.276 | 232 | 4 | 0 | 1 | 232 | 1 | 232 | 4.87e-170 | 467 |
| MS.gene48989.t1 | MTR_5g011800 | 52.119 | 236 | 102 | 5 | 1 | 232 | 1 | 229 | 2.96e-77 | 233 |
| MS.gene48989.t1 | MTR_4g102220 | 53.782 | 238 | 89 | 8 | 1 | 231 | 1 | 224 | 3.92e-73 | 222 |
| MS.gene48989.t1 | MTR_5g011820 | 51.261 | 238 | 101 | 6 | 1 | 232 | 4 | 232 | 6.36e-72 | 219 |
| MS.gene48989.t1 | MTR_7g117380 | 30.435 | 230 | 146 | 7 | 5 | 231 | 3 | 221 | 2.73e-23 | 94.0 |
| MS.gene48989.t1 | MTR_6g060390 | 29.310 | 232 | 136 | 8 | 5 | 231 | 4 | 212 | 7.63e-22 | 90.1 |
| MS.gene48989.t1 | MTR_1g076800 | 30.544 | 239 | 123 | 11 | 5 | 231 | 4 | 211 | 3.85e-21 | 88.2 |
| MS.gene48989.t1 | MTR_6g060440 | 28.879 | 232 | 135 | 9 | 5 | 231 | 4 | 210 | 1.51e-20 | 86.7 |
| MS.gene48989.t1 | MTR_4g023950 | 28.571 | 231 | 134 | 10 | 5 | 231 | 4 | 207 | 1.62e-18 | 81.3 |
| MS.gene48989.t1 | MTR_7g117380 | 30.055 | 183 | 118 | 6 | 52 | 231 | 4 | 179 | 1.62e-15 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48989.t1 | AT2G45360 | 54.545 | 231 | 88 | 4 | 1 | 231 | 1 | 214 | 5.66e-78 | 234 |
| MS.gene48989.t1 | AT3G60780 | 52.155 | 232 | 95 | 4 | 1 | 231 | 1 | 217 | 1.15e-74 | 226 |
| MS.gene48989.t1 | AT1G62840 | 44.958 | 238 | 111 | 6 | 1 | 232 | 1 | 224 | 2.45e-56 | 179 |
| MS.gene48989.t1 | AT1G12320 | 44.872 | 234 | 101 | 7 | 1 | 230 | 1 | 210 | 1.37e-46 | 154 |
Find 64 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAATGTTTCTTGGTTTAAA+TGG | 0.134381 | 4.3:+74274653 | MS.gene48989:CDS |
| ATAACATTCTACGATGAATT+TGG | 0.250717 | 4.3:-74274323 | None:intergenic |
| TATACATCATGAAGCTTGTT+TGG | 0.316497 | 4.3:+74273628 | None:intergenic |
| GGCAAAGGTCAAATGTTTCT+TGG | 0.321382 | 4.3:+74274644 | MS.gene48989:CDS |
| TGCAATCTTGACCCTTCATC+TGG | 0.322628 | 4.3:-74274427 | None:intergenic |
| AGGCCAACACTTGCGGCTAT+TGG | 0.324490 | 4.3:-74274358 | None:intergenic |
| TTTGCCTGTTGGAAAGGGTT+TGG | 0.337937 | 4.3:+74274720 | MS.gene48989:CDS |
| TTGTGGTGGACTGTAGGCTT+AGG | 0.342644 | 4.3:+74274551 | MS.gene48989:CDS |
| GAGTACTAGAGGGGCAGTTT+TGG | 0.356533 | 4.3:+74274606 | MS.gene48989:CDS |
| TGTGAGAAATTCAAGGAATC+TGG | 0.377584 | 4.3:+74274259 | MS.gene48989:CDS |
| TGTGGTGGACTGTAGGCTTA+GGG | 0.395659 | 4.3:+74274552 | MS.gene48989:CDS |
| GGGATACACCCATCTCGGCT+AGG | 0.414592 | 4.3:-74274462 | None:intergenic |
| ATTACAGTGTGAGAAATTCA+AGG | 0.434592 | 4.3:+74274252 | MS.gene48989:intron |
| ACGGTGATCAAAAGTCTTGA+TGG | 0.439976 | 4.3:+74274517 | MS.gene48989:CDS |
| GCTTACATAGGAAGTAGAAT+TGG | 0.450329 | 4.3:+74274748 | MS.gene48989:CDS |
| AGTTCTCGGTGAAGCGCAAA+CGG | 0.470434 | 4.3:+74274498 | MS.gene48989:CDS |
| GATGAATTTGGTGTTCCATC+CGG | 0.471577 | 4.3:-74274311 | None:intergenic |
| GGATACACCCATCTCGGCTA+GGG | 0.478620 | 4.3:-74274461 | None:intergenic |
| CACAAAAGCCCTAGCCGAGA+TGG | 0.481827 | 4.3:+74274453 | MS.gene48989:CDS |
| TTGAGGTTGAACAGTGCTTA+CGG | 0.484642 | 4.3:-74273694 | None:intergenic |
| ATATCCAAACCCTTTCCAAC+AGG | 0.485251 | 4.3:-74274724 | None:intergenic |
| AGCTTCAAGTAGTGCTTCAA+AGG | 0.491703 | 4.3:+74274780 | MS.gene48989:CDS |
| ATTGATCTTACTTGTTGTTG+AGG | 0.510185 | 4.3:-74273711 | None:intergenic |
| GATCAAAAGTCTTGATGGGT+TGG | 0.514141 | 4.3:+74274522 | MS.gene48989:CDS |
| CCGAGAACTATCTCCGGTGG+GGG | 0.517771 | 4.3:-74274484 | None:intergenic |
| GCAGTTGCTGCTCGCAACAC+CGG | 0.530079 | 4.3:+74274382 | MS.gene48989:CDS |
| AGGCACACGTGTGGTTAGAT+CGG | 0.531174 | 4.3:+74274693 | MS.gene48989:CDS |
| TGGAACATGGTTCTTGAAAG+AGG | 0.534421 | 4.3:+74274673 | MS.gene48989:CDS |
| TGTTGCGAGCAGCAACTGCT+AGG | 0.534968 | 4.3:-74274378 | None:intergenic |
| CACCGAGAACTATCTCCGGT+GGG | 0.537897 | 4.3:-74274486 | None:intergenic |
| GATCTATGAGTTTGATCCAA+CGG | 0.544545 | 4.3:-74274813 | None:intergenic |
| GGTTTGGATATTGCTTACAT+AGG | 0.545757 | 4.3:+74274736 | MS.gene48989:CDS |
| ACAAAAGCCCTAGCCGAGAT+GGG | 0.552505 | 4.3:+74274454 | MS.gene48989:CDS |
| GGTGTTCCATCCGGCTGCCA+TGG | 0.552686 | 4.3:-74274302 | None:intergenic |
| CGGTGATCAAAAGTCTTGAT+GGG | 0.555108 | 4.3:+74274518 | MS.gene48989:CDS |
| CTTCTATCATCCATGGCAGC+CGG | 0.557426 | 4.3:+74274292 | MS.gene48989:CDS |
| TACATAGGAAGTAGAATTGG+TGG | 0.559347 | 4.3:+74274751 | MS.gene48989:CDS |
| GCAATCTTGACCCTTCATCT+GGG | 0.562428 | 4.3:-74274426 | None:intergenic |
| ATCGTAGAATGTTATTCCCA+CGG | 0.567348 | 4.3:+74274331 | MS.gene48989:CDS |
| TATGCACACGTGTCTTGCAC+CGG | 0.569145 | 4.3:-74274401 | None:intergenic |
| GTGTGCATAGTCCCAGATGA+AGG | 0.570105 | 4.3:+74274415 | MS.gene48989:CDS |
| CGAGAACTATCTCCGGTGGG+GGG | 0.571171 | 4.3:-74274483 | None:intergenic |
| TCACCGAGAACTATCTCCGG+TGG | 0.572168 | 4.3:-74274487 | None:intergenic |
| TAGGGTTCTTAAGGTTGCAA+AGG | 0.574007 | 4.3:+74274582 | MS.gene48989:CDS |
| GGTCCAATAGCCGCAAGTGT+TGG | 0.576902 | 4.3:+74274355 | MS.gene48989:CDS |
| AGCTGAGCTTCTATCATCCA+TGG | 0.578564 | 4.3:+74274285 | MS.gene48989:CDS |
| TATCATCCATGGCAGCCGGA+TGG | 0.579782 | 4.3:+74274296 | MS.gene48989:CDS |
| TGCGGCTATTGGACCGCCGT+GGG | 0.584383 | 4.3:-74274347 | None:intergenic |
| CAAAGGGTACTCCTAGCCGT+TGG | 0.589404 | 4.3:+74274797 | MS.gene48989:CDS |
| GCTTCAAGTAGTGCTTCAAA+GGG | 0.589534 | 4.3:+74274781 | MS.gene48989:CDS |
| ACCGAGAACTATCTCCGGTG+GGG | 0.589901 | 4.3:-74274485 | None:intergenic |
| TTCTTGGTTTAAATGGAACA+TGG | 0.590885 | 4.3:+74274660 | MS.gene48989:CDS |
| GTTGCAAAGGTGAGTACTAG+AGG | 0.597632 | 4.3:+74274595 | MS.gene48989:CDS |
| AACTGCTAGGCCAACACTTG+CGG | 0.598354 | 4.3:-74274365 | None:intergenic |
| TTGCAAAGGTGAGTACTAGA+GGG | 0.599847 | 4.3:+74274596 | MS.gene48989:CDS |
| GAGAACTATCTCCGGTGGGG+GGG | 0.611049 | 4.3:-74274482 | None:intergenic |
| TTGCGGCTATTGGACCGCCG+TGG | 0.612852 | 4.3:-74274348 | None:intergenic |
| TGTGCATAGTCCCAGATGAA+GGG | 0.619561 | 4.3:+74274416 | MS.gene48989:CDS |
| ATGAGTTTGATCCAACGGCT+AGG | 0.623677 | 4.3:-74274808 | None:intergenic |
| ATAGGAAGTAGAATTGGTGG+TGG | 0.639207 | 4.3:+74274754 | MS.gene48989:CDS |
| GCTTCACCGAGAACTATCTC+CGG | 0.641092 | 4.3:-74274490 | None:intergenic |
| TGCAAAGGTGAGTACTAGAG+GGG | 0.660954 | 4.3:+74274597 | MS.gene48989:CDS |
| TCTTGAAAGAGGCACACGTG+TGG | 0.689378 | 4.3:+74274684 | MS.gene48989:CDS |
| GTAGAATGTTATTCCCACGG+CGG | 0.814857 | 4.3:+74274334 | MS.gene48989:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATGGTATTAATTATTT+TGG | + | chr4.3:74274221-74274240 | MS.gene48989:intron | 10.0% |
| !! | AGAAAAAAAATTATACAAAG+AGG | - | chr4.3:74274038-74274057 | None:intergenic | 15.0% |
| !! | GAAAAAAAATTATACAAAGA+GGG | - | chr4.3:74274037-74274056 | None:intergenic | 15.0% |
| !!! | AATTGATGATGTTTTTAATA+TGG | + | chr4.3:74274206-74274225 | MS.gene48989:intron | 15.0% |
| !! | AATTAAGACAAATAACAACA+AGG | - | chr4.3:74274175-74274194 | None:intergenic | 20.0% |
| ! | AAACTCATAGATCAAAAATC+AGG | + | chr4.3:74274823-74274842 | MS.gene48989:CDS | 25.0% |
| ! | ATAACATTCTACGATGAATT+TGG | - | chr4.3:74274326-74274345 | None:intergenic | 25.0% |
| ! | CAAATGTTTCTTGGTTTAAA+TGG | + | chr4.3:74274653-74274672 | MS.gene48989:CDS | 25.0% |
| AGGGAAAAGAAAATGGAAAA+AGG | - | chr4.3:74274018-74274037 | None:intergenic | 30.0% | |
| ATTACAGTGTGAGAAATTCA+AGG | + | chr4.3:74274252-74274271 | MS.gene48989:intron | 30.0% | |
| GGGAAAAGAAAATGGAAAAA+GGG | - | chr4.3:74274017-74274036 | None:intergenic | 30.0% | |
| TACAAAGAGGGAAAAGAAAA+TGG | - | chr4.3:74274025-74274044 | None:intergenic | 30.0% | |
| TTCTTGGTTTAAATGGAACA+TGG | + | chr4.3:74274660-74274679 | MS.gene48989:CDS | 30.0% | |
| ! | AGTGTCTATGTAAGCTTTTA+AGG | - | chr4.3:74273667-74273686 | None:intergenic | 30.0% |
| ! | CATATTTTGTGATGTGATTG+CGG | - | chr4.3:74274089-74274108 | None:intergenic | 30.0% |
| ! | TTTTGGCATGTAAAAATGCA+TGG | + | chr4.3:74274623-74274642 | MS.gene48989:CDS | 30.0% |
| !! | ATTGATCTTACTTGTTGTTG+AGG | - | chr4.3:74273714-74273733 | None:intergenic | 30.0% |
| ACATAGACTCATGTGATTGA+AGG | - | chr4.3:74273937-74273956 | None:intergenic | 35.0% | |
| ATAGACTCATGTGATTGAAG+GGG | - | chr4.3:74273935-74273954 | None:intergenic | 35.0% | |
| ATCGTAGAATGTTATTCCCA+CGG | + | chr4.3:74274331-74274350 | MS.gene48989:CDS | 35.0% | |
| CATAGACTCATGTGATTGAA+GGG | - | chr4.3:74273936-74273955 | None:intergenic | 35.0% | |
| CATGTAAAAATGCATGGCAA+AGG | + | chr4.3:74274629-74274648 | MS.gene48989:CDS | 35.0% | |
| CGCAATCACATCACAAAATA+TGG | + | chr4.3:74274087-74274106 | MS.gene48989:intron | 35.0% | |
| GATCTATGAGTTTGATCCAA+CGG | - | chr4.3:74274816-74274835 | None:intergenic | 35.0% | |
| GCTTACATAGGAAGTAGAAT+TGG | + | chr4.3:74274748-74274767 | MS.gene48989:CDS | 35.0% | |
| GGTTTGGATATTGCTTACAT+AGG | + | chr4.3:74274736-74274755 | MS.gene48989:CDS | 35.0% | |
| TACATAGGAAGTAGAATTGG+TGG | + | chr4.3:74274751-74274770 | MS.gene48989:CDS | 35.0% | |
| TGTGAGAAATTCAAGGAATC+TGG | + | chr4.3:74274259-74274278 | MS.gene48989:CDS | 35.0% | |
| !!! | GTTTTTTTGCCTGTTGGAAA+GGG | + | chr4.3:74274715-74274734 | MS.gene48989:CDS | 35.0% |
| !!! | TAAGCTTTTAAGGCAGTTTC+TGG | - | chr4.3:74273657-74273676 | None:intergenic | 35.0% |
| ACGGTGATCAAAAGTCTTGA+TGG | + | chr4.3:74274517-74274536 | MS.gene48989:CDS | 40.0% | |
| AGCTTCAAGTAGTGCTTCAA+AGG | + | chr4.3:74274780-74274799 | MS.gene48989:CDS | 40.0% | |
| ATAGGAAGTAGAATTGGTGG+TGG | + | chr4.3:74274754-74274773 | MS.gene48989:CDS | 40.0% | |
| ATATCCAAACCCTTTCCAAC+AGG | - | chr4.3:74274727-74274746 | None:intergenic | 40.0% | |
| CGGTGATCAAAAGTCTTGAT+GGG | + | chr4.3:74274518-74274537 | MS.gene48989:CDS | 40.0% | |
| GCTTCAAGTAGTGCTTCAAA+GGG | + | chr4.3:74274781-74274800 | MS.gene48989:CDS | 40.0% | |
| GGCAAAGGTCAAATGTTTCT+TGG | + | chr4.3:74274644-74274663 | MS.gene48989:CDS | 40.0% | |
| TAGGGTTCTTAAGGTTGCAA+AGG | + | chr4.3:74274582-74274601 | MS.gene48989:CDS | 40.0% | |
| TGGAACATGGTTCTTGAAAG+AGG | + | chr4.3:74274673-74274692 | MS.gene48989:CDS | 40.0% | |
| TTGAGGTTGAACAGTGCTTA+CGG | - | chr4.3:74273697-74273716 | None:intergenic | 40.0% | |
| TTGCAAAGGTGAGTACTAGA+GGG | + | chr4.3:74274596-74274615 | MS.gene48989:CDS | 40.0% | |
| ! | GATCAAAAGTCTTGATGGGT+TGG | + | chr4.3:74274522-74274541 | MS.gene48989:CDS | 40.0% |
| ! | GGATTTTGCTAGGGTTCTTA+AGG | + | chr4.3:74274573-74274592 | MS.gene48989:CDS | 40.0% |
| ! | TAGGCTTAGGGATTTTGCTA+GGG | + | chr4.3:74274564-74274583 | MS.gene48989:CDS | 40.0% |
| !! | GATGAATTTGGTGTTCCATC+CGG | - | chr4.3:74274314-74274333 | None:intergenic | 40.0% |
| !!! | AGATCGGTTTTTTTGCCTGT+TGG | + | chr4.3:74274709-74274728 | MS.gene48989:CDS | 40.0% |
| !!! | ATTTTCTTGTGGTGGACTGT+AGG | + | chr4.3:74274545-74274564 | MS.gene48989:CDS | 40.0% |
| !!! | GGTTTTTTTGCCTGTTGGAA+AGG | + | chr4.3:74274714-74274733 | MS.gene48989:CDS | 40.0% |
| !!! | TGATGGGTTGGATTTTCTTG+TGG | + | chr4.3:74274534-74274553 | MS.gene48989:CDS | 40.0% |
| AGCTGAGCTTCTATCATCCA+TGG | + | chr4.3:74274285-74274304 | MS.gene48989:CDS | 45.0% | |
| ATGAGTTTGATCCAACGGCT+AGG | - | chr4.3:74274811-74274830 | None:intergenic | 45.0% | |
| GCAATCTTGACCCTTCATCT+GGG | - | chr4.3:74274429-74274448 | None:intergenic | 45.0% | |
| GTAGAATGTTATTCCCACGG+CGG | + | chr4.3:74274334-74274353 | MS.gene48989:CDS | 45.0% | |
| GTTGCAAAGGTGAGTACTAG+AGG | + | chr4.3:74274595-74274614 | MS.gene48989:CDS | 45.0% | |
| TGCAAAGGTGAGTACTAGAG+GGG | + | chr4.3:74274597-74274616 | MS.gene48989:CDS | 45.0% | |
| TGCAATCTTGACCCTTCATC+TGG | - | chr4.3:74274430-74274449 | None:intergenic | 45.0% | |
| TGTGCATAGTCCCAGATGAA+GGG | + | chr4.3:74274416-74274435 | MS.gene48989:CDS | 45.0% | |
| TTTGCCTGTTGGAAAGGGTT+TGG | + | chr4.3:74274720-74274739 | MS.gene48989:CDS | 45.0% | |
| ! | GTAGGCTTAGGGATTTTGCT+AGG | + | chr4.3:74274563-74274582 | MS.gene48989:CDS | 45.0% |
| !! | TGGGTTGGATTTTCTTGTGG+TGG | + | chr4.3:74274537-74274556 | MS.gene48989:CDS | 45.0% |
| !! | CAAAATAAAAATAAAAATAT+GGG | - | chr4.3:74273737-74273756 | None:intergenic | 5.0% |
| !! | TCAAAATAAAAATAAAAATA+TGG | - | chr4.3:74273738-74273757 | None:intergenic | 5.0% |
| !!! | TATATAATATTTTGAAATAT+TGG | - | chr4.3:74273809-74273828 | None:intergenic | 5.0% |
| AACTGCTAGGCCAACACTTG+CGG | - | chr4.3:74274368-74274387 | None:intergenic | 50.0% | |
| ACAAAAGCCCTAGCCGAGAT+GGG | + | chr4.3:74274454-74274473 | MS.gene48989:CDS | 50.0% | |
| AGGCACACGTGTGGTTAGAT+CGG | + | chr4.3:74274693-74274712 | MS.gene48989:CDS | 50.0% | |
| AGTTCTCGGTGAAGCGCAAA+CGG | + | chr4.3:74274498-74274517 | MS.gene48989:CDS | 50.0% | |
| CTTCTATCATCCATGGCAGC+CGG | + | chr4.3:74274292-74274311 | MS.gene48989:CDS | 50.0% | |
| GCTTCACCGAGAACTATCTC+CGG | - | chr4.3:74274493-74274512 | None:intergenic | 50.0% | |
| GTGTGCATAGTCCCAGATGA+AGG | + | chr4.3:74274415-74274434 | MS.gene48989:CDS | 50.0% | |
| TATGCACACGTGTCTTGCAC+CGG | - | chr4.3:74274404-74274423 | None:intergenic | 50.0% | |
| ! | GAGTACTAGAGGGGCAGTTT+TGG | + | chr4.3:74274606-74274625 | MS.gene48989:CDS | 50.0% |
| ! | TCTTGAAAGAGGCACACGTG+TGG | + | chr4.3:74274684-74274703 | MS.gene48989:CDS | 50.0% |
| !! | TGTGGTGGACTGTAGGCTTA+GGG | + | chr4.3:74274552-74274571 | MS.gene48989:CDS | 50.0% |
| !! | TTGTGGTGGACTGTAGGCTT+AGG | + | chr4.3:74274551-74274570 | MS.gene48989:CDS | 50.0% |
| ACCGAGAACTATCTCCGGTG+GGG | - | chr4.3:74274488-74274507 | None:intergenic | 55.0% | |
| AGGCCAACACTTGCGGCTAT+TGG | - | chr4.3:74274361-74274380 | None:intergenic | 55.0% | |
| CACAAAAGCCCTAGCCGAGA+TGG | + | chr4.3:74274453-74274472 | MS.gene48989:CDS | 55.0% | |
| CACCGAGAACTATCTCCGGT+GGG | - | chr4.3:74274489-74274508 | None:intergenic | 55.0% | |
| GGATACACCCATCTCGGCTA+GGG | - | chr4.3:74274464-74274483 | None:intergenic | 55.0% | |
| GGTCCAATAGCCGCAAGTGT+TGG | + | chr4.3:74274355-74274374 | MS.gene48989:CDS | 55.0% | |
| TATCATCCATGGCAGCCGGA+TGG | + | chr4.3:74274296-74274315 | MS.gene48989:CDS | 55.0% | |
| TCACCGAGAACTATCTCCGG+TGG | - | chr4.3:74274490-74274509 | None:intergenic | 55.0% | |
| TGTTGCGAGCAGCAACTGCT+AGG | - | chr4.3:74274381-74274400 | None:intergenic | 55.0% | |
| !! | CAAAGGGTACTCCTAGCCGT+TGG | + | chr4.3:74274797-74274816 | MS.gene48989:CDS | 55.0% |
| CCCCCACCGGAGATAGTTCT+CGG | + | chr4.3:74274484-74274503 | MS.gene48989:CDS | 60.0% | |
| CCGAGAACTATCTCCGGTGG+GGG | - | chr4.3:74274487-74274506 | None:intergenic | 60.0% | |
| CGAGAACTATCTCCGGTGGG+GGG | - | chr4.3:74274486-74274505 | None:intergenic | 60.0% | |
| GAGAACTATCTCCGGTGGGG+GGG | - | chr4.3:74274485-74274504 | None:intergenic | 60.0% | |
| GCAGTTGCTGCTCGCAACAC+CGG | + | chr4.3:74274382-74274401 | MS.gene48989:CDS | 60.0% | |
| GGGATACACCCATCTCGGCT+AGG | - | chr4.3:74274465-74274484 | None:intergenic | 60.0% | |
| TGGGGGGGATACACCCATCT+CGG | - | chr4.3:74274470-74274489 | None:intergenic | 60.0% | |
| GATGGGTGTATCCCCCCCAC+CGG | + | chr4.3:74274471-74274490 | MS.gene48989:CDS | 65.0% | |
| GGTGTTCCATCCGGCTGCCA+TGG | - | chr4.3:74274305-74274324 | None:intergenic | 65.0% | |
| TGCGGCTATTGGACCGCCGT+GGG | - | chr4.3:74274350-74274369 | None:intergenic | 65.0% | |
| TTGCGGCTATTGGACCGCCG+TGG | - | chr4.3:74274351-74274370 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 74273636 | 74274870 | 74273636 | ID=MS.gene48989 |
| chr4.3 | mRNA | 74273636 | 74274870 | 74273636 | ID=MS.gene48989.t1;Parent=MS.gene48989 |
| chr4.3 | exon | 74273636 | 74273722 | 74273636 | ID=MS.gene48989.t1.exon1;Parent=MS.gene48989.t1 |
| chr4.3 | CDS | 74273636 | 74273722 | 74273636 | ID=cds.MS.gene48989.t1;Parent=MS.gene48989.t1 |
| chr4.3 | exon | 74274259 | 74274870 | 74274259 | ID=MS.gene48989.t1.exon2;Parent=MS.gene48989.t1 |
| chr4.3 | CDS | 74274259 | 74274870 | 74274259 | ID=cds.MS.gene48989.t1;Parent=MS.gene48989.t1 |
| Gene Sequence |
| Protein sequence |
