AlfalfaGEDB | MS.gene74452

M. sativa cultivar XinJiangDaYe / MS.gene74452

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9 PF13833.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74452.t1	XP_003618823.1	98.6	142	2	0	1	142	1	142	8.40E-74	286.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74452.t1	Q8RYJ8	54.3	138	59	2	8	141	7	144	7.3e-34	144.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74452.t1	G7KNL8	98.6	142	2	0	1	142	1	142	6.0e-74	286.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049567	MS.gene74452	0.80239	5.74E-49	-1.69E-46
MS.gene052493	MS.gene74452	0.809496	1.84E-50	-1.69E-46
MS.gene05439	MS.gene74452	0.813469	2.52E-51	-1.69E-46
MS.gene060412	MS.gene74452	0.826707	2.34E-54	-1.69E-46
MS.gene061047	MS.gene74452	0.815214	1.04E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene74452	MS.gene64310	PPI
MS.gene74452	MS.gene29459	PPI
MS.gene74452	MS.gene71207	PPI
MS.gene038456	MS.gene74452	PPI
MS.gene004899	MS.gene74452	PPI
MS.gene25186	MS.gene74452	PPI
MS.gene009021	MS.gene74452	PPI
MS.gene070167	MS.gene74452	PPI
MS.gene74452	MS.gene21696	PPI
MS.gene74452	MS.gene074045	PPI
MS.gene74452	MS.gene03228	PPI
MS.gene04915	MS.gene74452	PPI
MS.gene74452	MS.gene85596	PPI
MS.gene74452	MS.gene46237	PPI
MS.gene74452	MS.gene64317	PPI
MS.gene63281	MS.gene74452	PPI
MS.gene74452	MS.gene94551	PPI
MS.gene68345	MS.gene74452	PPI
MS.gene036930	MS.gene74452	PPI
MS.gene001246	MS.gene74452	PPI
MS.gene74452	MS.gene93087	PPI
MS.gene040127	MS.gene74452	PPI
MS.gene62258	MS.gene74452	PPI
MS.gene74452	MS.gene046988	PPI
MS.gene74452	MS.gene057293	PPI
MS.gene55545	MS.gene74452	PPI
MS.gene000724	MS.gene74452	PPI
MS.gene052672	MS.gene74452	PPI
MS.gene74452	MS.gene09934	PPI
MS.gene74452	MS.gene79028	PPI
MS.gene64311	MS.gene74452	PPI
MS.gene58906	MS.gene74452	PPI
MS.gene020515	MS.gene74452	PPI
MS.gene74452	MS.gene026940	PPI
MS.gene74452	MS.gene65954	PPI
MS.gene58860	MS.gene74452	PPI
MS.gene64313	MS.gene74452	PPI
MS.gene83068	MS.gene74452	PPI
MS.gene27516	MS.gene74452	PPI
MS.gene62689	MS.gene74452	PPI
MS.gene053319	MS.gene74452	PPI
MS.gene97948	MS.gene74452	PPI
MS.gene74452	MS.gene96922	PPI
MS.gene61009	MS.gene74452	PPI
MS.gene64327	MS.gene74452	PPI
MS.gene000738	MS.gene74452	PPI
MS.gene74452	MS.gene80013	PPI
MS.gene64307	MS.gene74452	PPI
MS.gene74452	MS.gene025797	PPI
MS.gene74452	MS.gene21931	PPI
MS.gene061261	MS.gene74452	PPI
MS.gene74452	MS.gene64314	PPI
MS.gene04917	MS.gene74452	PPI
MS.gene74452	MS.gene53659	PPI
MS.gene74452	MS.gene09960	PPI
MS.gene026530	MS.gene74452	PPI
MS.gene013748	MS.gene74452	PPI
MS.gene74452	MS.gene017446	PPI
MS.gene74452	MS.gene53650	PPI
MS.gene74452	MS.gene066863	PPI
MS.gene74452	MS.gene02545	PPI
MS.gene74452	MS.gene96913	PPI
MS.gene28338	MS.gene74452	PPI
MS.gene008225	MS.gene74452	PPI
MS.gene74452	MS.gene93763	PPI
MS.gene64324	MS.gene74452	PPI
MS.gene74452	MS.gene80012	PPI
MS.gene015470	MS.gene74452	PPI
MS.gene015658	MS.gene74452	PPI
MS.gene024717	MS.gene74452	PPI
MS.gene64308	MS.gene74452	PPI
MS.gene53662	MS.gene74452	PPI
MS.gene003880	MS.gene74452	PPI
MS.gene54637	MS.gene74452	PPI
MS.gene67281	MS.gene74452	PPI
MS.gene025777	MS.gene74452	PPI
MS.gene74452	MS.gene98972	PPI
MS.gene062239	MS.gene74452	PPI
MS.gene46886	MS.gene74452	PPI
MS.gene74452	MS.gene061768	PPI
MS.gene74452	MS.gene89216	PPI
MS.gene74452	MS.gene64308	PPI
MS.gene74452	MS.gene47195	PPI
MS.gene21155	MS.gene74452	PPI
MS.gene92775	MS.gene74452	PPI
MS.gene74452	MS.gene96914	PPI
MS.gene74452	MS.gene066862	PPI
MS.gene21932	MS.gene74452	PPI
MS.gene74452	MS.gene07422	PPI
MS.gene056383	MS.gene74452	PPI
MS.gene74452	MS.gene64316	PPI
MS.gene87673	MS.gene74452	PPI
MS.gene05284	MS.gene74452	PPI
MS.gene041795	MS.gene74452	PPI
MS.gene63000	MS.gene74452	PPI
MS.gene22553	MS.gene74452	PPI
MS.gene74452	MS.gene026618	PPI
MS.gene80011	MS.gene74452	PPI
MS.gene74452	MS.gene53648	PPI
MS.gene53648	MS.gene74452	PPI
MS.gene070168	MS.gene74452	PPI
MS.gene74452	MS.gene008831	PPI
MS.gene74452	MS.gene056461	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74452.t1	MTR_6g023460	98.592	142	2	0	1	142	1	142	5.42e-100	283
MS.gene74452.t1	MTR_4g103655	61.194	134	52	0	8	141	6	139	3.07e-56	172
MS.gene74452.t1	MTR_4g103650	60.448	134	53	0	8	141	2	135	2.53e-55	170
MS.gene74452.t1	MTR_4g103630	59.701	134	54	0	8	141	6	139	4.21e-55	169
MS.gene74452.t1	MTR_3g089070	57.554	139	59	0	3	141	1	139	2.45e-54	167
MS.gene74452.t1	MTR_3g089090	57.664	137	58	0	5	141	2	138	4.24e-54	167
MS.gene74452.t1	MTR_8g078270	54.135	133	56	3	12	141	57	187	8.47e-40	132
MS.gene74452.t1	MTR_1g046950	42.029	138	69	2	11	141	7	140	2.00e-32	112
MS.gene74452.t1	MTR_3g067610	42.748	131	72	1	11	141	8	135	8.56e-32	110
MS.gene74452.t1	MTR_5g079340	42.105	133	73	2	11	141	7	137	1.99e-31	109
MS.gene74452.t1	MTR_8g107110	41.538	130	76	0	12	141	28	157	2.87e-29	104
MS.gene74452.t1	MTR_1g047100	39.416	137	73	2	11	141	7	139	3.30e-28	101
MS.gene74452.t1	MTR_4g067270	39.552	134	78	2	11	141	8	141	1.35e-26	97.8
MS.gene74452.t1	MTR_8g105230	40.625	128	75	1	12	138	40	167	1.37e-25	96.3
MS.gene74452.t1	MTR_6g079570	40.909	132	77	1	11	141	29	160	1.61e-25	95.5
MS.gene74452.t1	MTR_1g032070	36.842	133	83	1	11	142	49	181	4.33e-24	92.4
MS.gene74452.t1	MTR_7g075040	38.667	150	80	4	3	141	42	190	1.21e-23	91.3
MS.gene74452.t1	MTR_8g036075	37.063	143	81	3	8	141	49	191	1.25e-22	88.6
MS.gene74452.t1	MTR_8g066630	31.159	138	91	1	4	137	13	150	5.67e-22	86.3
MS.gene74452.t1	MTR_5g017550	32.824	131	88	0	12	142	52	182	2.00e-21	85.5
MS.gene74452.t1	MTR_5g008705	36.641	131	74	2	16	141	2	128	3.09e-21	83.2
MS.gene74452.t1	MTR_1g076650	32.143	140	94	1	4	142	8	147	4.35e-21	83.6
MS.gene74452.t1	MTR_1g076650	40.299	67	40	0	75	141	7	73	9.08e-12	59.3
MS.gene74452.t1	MTR_5g017510	34.091	132	86	1	12	142	52	183	9.32e-21	83.6
MS.gene74452.t1	MTR_5g017560	34.091	132	86	1	12	142	52	183	9.32e-21	83.6
MS.gene74452.t1	MTR_7g087610	28.571	140	99	1	4	142	8	147	4.07e-20	80.9
MS.gene74452.t1	MTR_7g089760	35.075	134	84	2	11	141	8	141	5.25e-20	80.9
MS.gene74452.t1	MTR_4g086260	33.846	130	85	1	12	141	61	189	5.82e-20	81.6
MS.gene74452.t1	MTR_1g041285	31.429	140	95	1	4	142	8	147	3.50e-19	78.6
MS.gene74452.t1	MTR_1g071150	28.169	142	102	0	1	142	5	146	3.80e-19	78.6
MS.gene74452.t1	MTR_6g025320	27.857	140	100	1	4	142	8	147	1.29e-18	77.0
MS.gene74452.t1	MTR_4g112460	30.282	142	96	1	3	141	6	147	2.31e-18	77.0
MS.gene74452.t1	MTR_5g088320	27.143	140	101	1	4	142	8	147	5.99e-18	75.5
MS.gene74452.t1	MTR_5g088320	37.313	67	42	0	75	141	7	73	7.30e-11	57.0
MS.gene74452.t1	MTR_2g098890	35.294	136	83	2	11	141	67	202	3.67e-17	74.7
MS.gene74452.t1	MTR_4g115170	34.667	150	79	3	11	141	67	216	5.23e-17	74.7
MS.gene74452.t1	MTR_5g027000	29.078	141	99	1	2	141	16	156	6.37e-17	73.2
MS.gene74452.t1	MTR_5g008695	34.783	115	64	2	33	140	2	112	9.27e-17	71.2
MS.gene74452.t1	MTR_7g074240	32.639	144	84	2	11	141	27	170	2.14e-16	72.0
MS.gene74452.t1	MTR_3g083470	32.331	133	79	3	11	142	96	218	2.25e-16	73.2
MS.gene74452.t1	MTR_8g069915	26.950	141	100	1	4	141	8	148	7.99e-16	70.5
MS.gene74452.t1	MTR_1g030440	35.211	142	75	3	12	142	26	161	8.35e-16	70.1
MS.gene74452.t1	MTR_1g026190	27.068	133	92	2	11	141	390	519	5.91e-13	65.1
MS.gene74452.t1	MTR_7g090450	29.801	151	92	4	1	141	66	212	1.70e-12	62.4
MS.gene74452.t1	MTR_3g088655	32.903	155	79	5	7	142	7	155	1.12e-11	59.3
MS.gene74452.t1	MTR_6g007613	29.771	131	91	1	12	141	33	163	1.22e-11	59.3
MS.gene74452.t1	MTR_5g089320	27.820	133	91	2	11	141	376	505	1.26e-11	61.2
MS.gene74452.t1	MTR_5g009830	27.273	132	91	2	12	141	375	503	1.95e-11	60.8
MS.gene74452.t1	MTR_8g099095	28.030	132	90	2	12	141	375	503	2.51e-11	60.5
MS.gene74452.t1	MTR_5g079470	39.683	63	37	1	11	73	13	74	2.62e-11	56.6
MS.gene74452.t1	MTR_0168s0120	26.606	109	79	1	35	142	3	111	3.46e-11	57.0
MS.gene74452.t1	MTR_6g025320	26.606	109	79	1	35	142	3	111	3.46e-11	57.0
MS.gene74452.t1	MTR_7g074020	29.008	131	92	1	12	141	34	164	4.06e-11	58.2
MS.gene74452.t1	MTR_7g068710	28.462	130	93	0	12	141	365	494	5.67e-11	59.3
MS.gene74452.t1	MTR_5g092810	28.030	132	90	2	12	141	401	529	6.33e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74452.t1	AT1G76650	49.275	138	70	0	4	141	39	176	4.93e-42	137
MS.gene74452.t1	AT1G76650	49.275	138	70	0	4	141	39	176	4.93e-42	137
MS.gene74452.t1	AT1G76640	50.376	133	63	1	12	141	26	158	2.45e-40	132
MS.gene74452.t1	AT3G50770	48.175	137	65	2	11	141	67	203	5.84e-38	128
MS.gene74452.t1	AT5G42380	49.275	138	68	1	6	141	47	184	4.18e-37	125
MS.gene74452.t1	AT3G01830	48.592	142	68	3	3	141	6	145	2.02e-36	122
MS.gene74452.t1	AT1G76650	44.928	138	66	1	4	141	39	166	1.93e-35	120
MS.gene74452.t1	AT5G37770	43.382	136	72	1	11	141	20	155	1.12e-31	110
MS.gene74452.t1	AT5G37770	43.382	136	72	1	11	141	20	155	1.12e-31	110
MS.gene74452.t1	AT1G66400	37.778	135	81	1	11	142	18	152	2.04e-27	100
MS.gene74452.t1	AT1G24620	39.695	131	78	1	12	141	41	171	5.83e-26	97.1
MS.gene74452.t1	AT1G18210	38.168	131	78	1	11	141	26	153	7.06e-25	94.0
MS.gene74452.t1	AT1G18210	38.168	131	78	1	11	141	26	153	7.06e-25	94.0
MS.gene74452.t1	AT5G17470	36.567	134	80	2	12	141	6	138	2.11e-24	92.0
MS.gene74452.t1	AT1G73630	36.641	131	80	1	11	141	23	150	4.86e-24	91.7
MS.gene74452.t1	AT1G05990	37.410	139	83	2	7	141	4	142	6.12e-24	90.9
MS.gene74452.t1	AT2G43290	35.211	142	78	3	11	141	68	206	3.23e-22	88.2
MS.gene74452.t1	AT3G59440	35.338	133	84	1	11	141	54	186	1.15e-21	86.3
MS.gene74452.t1	AT4G12860	37.313	134	81	2	11	141	8	141	1.42e-21	84.7
MS.gene74452.t1	AT4G03290	35.461	141	85	2	7	141	4	144	1.87e-21	84.7
MS.gene74452.t1	AT3G07490	36.567	134	82	2	11	141	8	141	1.32e-20	82.4
MS.gene74452.t1	AT2G36180	35.036	137	81	3	11	141	3	137	3.39e-20	81.3
MS.gene74452.t1	AT2G15680	33.333	138	87	2	6	141	48	182	3.75e-20	82.0
MS.gene74452.t1	AT2G41100	30.263	152	88	1	4	137	62	213	8.50e-20	83.2
MS.gene74452.t1	AT2G41100	30.263	152	88	1	4	137	62	213	8.50e-20	83.2
MS.gene74452.t1	AT2G41100	30.263	152	88	1	4	137	62	213	8.50e-20	83.2
MS.gene74452.t1	AT2G41100	30.263	152	88	1	4	137	97	248	1.25e-19	83.2
MS.gene74452.t1	AT2G41100	29.677	155	92	1	4	141	8	162	1.91e-19	82.8
MS.gene74452.t1	AT2G41100	30.263	152	88	1	4	137	97	248	1.25e-19	83.2
MS.gene74452.t1	AT2G41100	29.677	155	92	1	4	141	8	162	1.91e-19	82.8
MS.gene74452.t1	AT4G14640	31.293	147	92	2	1	142	6	148	2.27e-19	79.3
MS.gene74452.t1	AT3G01830	45.045	111	54	3	3	109	6	113	3.36e-19	78.2
MS.gene74452.t1	AT3G22930	28.671	143	101	1	1	142	28	170	5.93e-19	78.6
MS.gene74452.t1	AT5G21274	28.571	140	99	1	4	142	8	147	5.99e-19	78.2
MS.gene74452.t1	AT3G56800	27.857	140	100	1	4	142	8	147	1.15e-18	77.4
MS.gene74452.t1	AT2G27030	27.857	140	100	1	4	142	8	147	1.15e-18	77.4
MS.gene74452.t1	AT2G41110	27.857	140	100	1	4	142	8	147	1.15e-18	77.4
MS.gene74452.t1	AT3G43810	27.857	140	100	1	4	142	8	147	1.35e-18	77.0
MS.gene74452.t1	AT5G37780	27.143	140	101	1	4	142	8	147	1.81e-18	76.6
MS.gene74452.t1	AT5G37780	37.313	67	42	0	75	141	7	73	7.33e-11	57.0
MS.gene74452.t1	AT1G66410	27.143	140	101	1	4	142	8	147	1.81e-18	76.6
MS.gene74452.t1	AT1G66410	37.313	67	42	0	75	141	7	73	7.33e-11	57.0
MS.gene74452.t1	AT1G18530	31.206	141	94	2	4	141	3	143	2.02e-18	77.0
MS.gene74452.t1	AT2G27030	27.857	140	100	1	4	142	8	147	3.79e-18	76.6
MS.gene74452.t1	AT3G43810	27.857	140	100	1	4	142	8	147	7.51e-18	76.6
MS.gene74452.t1	AT1G32250	29.787	141	92	2	4	137	12	152	7.75e-17	73.2
MS.gene74452.t1	AT3G03400	34.884	129	75	4	12	134	9	134	8.86e-17	72.0
MS.gene74452.t1	AT3G50360	32.824	131	87	1	12	141	31	161	9.58e-17	72.8
MS.gene74452.t1	AT2G41110	25.658	152	100	2	4	142	8	159	2.23e-16	71.6
MS.gene74452.t1	AT3G25600	29.078	141	97	2	4	141	8	148	5.68e-16	70.5
MS.gene74452.t1	AT4G37010	29.771	131	91	1	12	141	35	165	1.72e-15	69.7
MS.gene74452.t1	AT4G37010	29.771	131	91	1	12	141	31	161	1.82e-15	69.3
MS.gene74452.t1	AT3G51920	27.143	140	101	1	4	142	8	147	1.05e-14	67.0
MS.gene74452.t1	AT3G03000	30.147	136	93	1	4	137	16	151	1.09e-14	67.4
MS.gene74452.t1	AT5G37780	24.516	155	101	2	4	142	8	162	1.90e-14	66.6
MS.gene74452.t1	AT3G10190	28.676	136	95	2	8	141	70	205	2.46e-14	67.4
MS.gene74452.t1	AT4G14640	30.952	126	78	2	22	142	9	130	1.14e-13	63.9
MS.gene74452.t1	AT2G41410	30.070	143	96	3	4	142	66	208	2.54e-13	64.7
MS.gene74452.t1	AT3G03410	30.303	132	84	1	11	142	5	128	3.09e-13	62.8
MS.gene74452.t1	AT2G41090	24.638	138	102	1	5	142	9	144	3.95e-13	63.9
MS.gene74452.t1	AT5G37780	27.049	122	88	1	22	142	13	134	4.49e-13	62.4
MS.gene74452.t1	AT4G04695	28.462	130	92	1	12	141	340	468	4.50e-13	65.5
MS.gene74452.t1	AT1G66410	27.049	122	88	1	22	142	36	157	6.97e-13	62.4
MS.gene74452.t1	AT5G37780	27.049	122	88	1	22	142	52	173	8.03e-13	62.8
MS.gene74452.t1	AT1G12310	31.206	141	92	2	4	142	9	146	8.87e-13	62.0
MS.gene74452.t1	AT4G20780	30.921	152	84	3	11	141	32	183	2.12e-12	62.0
MS.gene74452.t1	AT1G62820	30.496	141	93	2	4	142	9	146	2.99e-12	60.5
MS.gene74452.t1	AT3G51850	32.331	133	84	3	12	141	363	492	5.69e-12	62.4
MS.gene74452.t1	AT1G66410	26.606	109	79	1	35	142	3	111	1.14e-11	58.2
MS.gene74452.t1	AT1G66410	26.606	109	79	1	35	142	3	111	1.14e-11	58.2
MS.gene74452.t1	AT4G04700	28.788	132	89	2	12	141	340	468	1.75e-11	60.8
MS.gene74452.t1	AT2G31500	29.078	141	97	2	4	141	367	507	1.83e-11	60.8
MS.gene74452.t1	AT1G76040	30.075	133	88	2	11	141	181	310	1.87e-11	60.5
MS.gene74452.t1	AT1G76040	30.075	133	88	2	11	141	181	310	1.87e-11	60.5
MS.gene74452.t1	AT1G74740	30.534	131	89	2	12	141	368	497	2.92e-11	60.5
MS.gene74452.t1	AT1G76040	30.075	133	88	2	11	141	419	548	3.40e-11	60.1
MS.gene74452.t1	AT3G43810	26.606	109	79	1	35	142	3	111	3.62e-11	57.0
MS.gene74452.t1	AT3G43810	26.606	109	79	1	35	142	3	111	3.62e-11	57.0
MS.gene74452.t1	AT5G12180	29.545	132	88	2	12	141	382	510	4.67e-11	59.7
MS.gene74452.t1	AT5G19360	28.788	132	89	2	12	141	377	505	8.15e-11	58.9
MS.gene74452.t1	AT4G23650	28.030	132	90	2	12	141	387	515	8.41e-11	58.9

Find 44 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAACCACTTTGATGAAAA+TGG	0.221308	6.4:-47645	MS.gene74452:CDS
ATTTCTCAATTTGACATTGA+TGG	0.261073	6.4:-47321	MS.gene74452:CDS
CGCCTCCTCGTTCGACATCT+TGG	0.273549	6.4:+47569	None:intergenic
AGCATTCTCTTAAGACTCTT+TGG	0.286525	6.4:+47390	None:intergenic
TAAGAGAATGCTTGGAAGAT+TGG	0.354871	6.4:-47379	MS.gene74452:CDS
GTCGAACGAGGAGGCGGAAA+TGG	0.371555	6.4:-47562	MS.gene74452:CDS
CGGCAATGTGTGGAGGCAAT+CGG	0.378283	6.4:-47594	MS.gene74452:CDS
CAATTTGACATTGATGGAGA+TGG	0.399838	6.4:-47315	MS.gene74452:CDS
GGCAATGTGTGGAGGCAATC+GGG	0.408198	6.4:-47593	MS.gene74452:CDS
GTTTGTAGAGGGAGGGAAAG+AGG	0.418412	6.4:-47481	MS.gene74452:CDS
AAGAGAATGCTTGGAAGATT+GGG	0.420123	6.4:-47378	MS.gene74452:CDS
TTCTCGACTCGGACGGGGAT+GGG	0.440025	6.4:-47530	MS.gene74452:CDS
TCGGACGGGGATGGGTTAGT+TGG	0.440699	6.4:-47522	MS.gene74452:CDS
TTGAGCTTCTCGACTCGGAC+GGG	0.442068	6.4:-47536	MS.gene74452:CDS
CGGGGATGGGTTAGTTGGGT+TGG	0.451530	6.4:-47517	MS.gene74452:CDS
CGGACGGGGATGGGTTAGTT+GGG	0.453908	6.4:-47521	MS.gene74452:CDS
AAGAGTCTTAAGAGAATGCT+TGG	0.464880	6.4:-47387	MS.gene74452:CDS
TTGTGAAGTTTGTAGAGGGA+GGG	0.474275	6.4:-47488	MS.gene74452:CDS
TCCTAAATTCATCAAAACTA+AGG	0.475085	6.4:+47289	None:intergenic
CTTCTCGACTCGGACGGGGA+TGG	0.478951	6.4:-47531	MS.gene74452:CDS
AATTTGACATTGATGGAGAT+GGG	0.498825	6.4:-47314	MS.gene74452:CDS
TTTGTGAAGTTTGTAGAGGG+AGG	0.500405	6.4:-47489	MS.gene74452:CDS
ACATCTTAAAAGCTTCCCTT+AGG	0.513431	6.4:+47442	None:intergenic
AAGAGAAAGTGAATGACCTA+AGG	0.518435	6.4:-47458	MS.gene74452:CDS
GGCGGTTGAGCTTCTCGACT+CGG	0.522065	6.4:-47541	MS.gene74452:CDS
CACTTTGATGAAAATGGAGA+TGG	0.524653	6.4:-47639	MS.gene74452:CDS
GTCGGAGCTGCGGCAATGTG+TGG	0.526902	6.4:-47604	MS.gene74452:CDS
CAAGATGTCGAACGAGGAGG+CGG	0.533170	6.4:-47568	MS.gene74452:CDS
TTTAAGATGTATGAAATGGA+GGG	0.540787	6.4:-47429	MS.gene74452:CDS
GTTGAGCTTCTCGACTCGGA+CGG	0.543058	6.4:-47537	MS.gene74452:CDS
TGAGCTTCTCGACTCGGACG+GGG	0.549367	6.4:-47535	MS.gene74452:CDS
AGAGAAAGTGAATGACCTAA+GGG	0.570683	6.4:-47457	MS.gene74452:CDS
ACACATTGCCGCAGCTCCGA+CGG	0.580783	6.4:+47606	None:intergenic
TATAGATATGGTCATGGACA+AGG	0.584911	6.4:-47688	None:intergenic
AAATATCACCGTCGGAGCTG+CGG	0.585133	6.4:-47614	MS.gene74452:CDS
ATGTATGAAATGGAGGGGTG+TGG	0.585987	6.4:-47423	MS.gene74452:CDS
TTAAGATGTATGAAATGGAG+GGG	0.595061	6.4:-47428	MS.gene74452:CDS
GAACGAGGAGGCGGAAATGG+CGG	0.597662	6.4:-47559	MS.gene74452:CDS
GCAATGTGTGGAGGCAATCG+GGG	0.601893	6.4:-47592	MS.gene74452:CDS
GGAGCTGCGGCAATGTGTGG+AGG	0.609680	6.4:-47601	MS.gene74452:CDS
AAATTATATAGATATGGTCA+TGG	0.623907	6.4:-47694	None:intergenic
ATTTGACATTGATGGAGATG+GGG	0.633515	6.4:-47313	MS.gene74452:CDS
CGGGGCCAAGATGTCGAACG+AGG	0.661203	6.4:-47574	MS.gene74452:CDS
GGCCAAGATGTCGAACGAGG+AGG	0.678288	6.4:-47571	MS.gene74452:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTTCTCAATTTGACATTGA+TGG	-	chr6.4:47635-47654	MS.gene74452:CDS	25.0%
!	TCCTAAATTCATCAAAACTA+AGG	+	chr6.4:47670-47689	None:intergenic	25.0%
!!	TTTAAGATGTATGAAATGGA+GGG	-	chr6.4:47527-47546	MS.gene74452:CDS	25.0%
!!!	AGCTTTTAAGATGTATGAAA+TGG	-	chr6.4:47523-47542	MS.gene74452:CDS	25.0%
!!!	TCCTTAGTTTTGATGAATTT+AGG	-	chr6.4:47666-47685	MS.gene74452:CDS	25.0%
!!!	TTTTAAGATGTATGAAATGG+AGG	-	chr6.4:47526-47545	MS.gene74452:CDS	25.0%
	AATTTGACATTGATGGAGAT+GGG	-	chr6.4:47642-47661	MS.gene74452:CDS	30.0%
	TTAAGATGTATGAAATGGAG+GGG	-	chr6.4:47528-47547	MS.gene74452:CDS	30.0%
	TTCAACCACTTTGATGAAAA+TGG	-	chr6.4:47311-47330	MS.gene74452:CDS	30.0%
!	GATTTTGTGAAGTTTGTAGA+GGG	-	chr6.4:47464-47483	MS.gene74452:CDS	30.0%
!	TGATTTTGTGAAGTTTGTAG+AGG	-	chr6.4:47463-47482	MS.gene74452:CDS	30.0%
	AAGAGAAAGTGAATGACCTA+AGG	-	chr6.4:47498-47517	MS.gene74452:CDS	35.0%
	AAGAGAATGCTTGGAAGATT+GGG	-	chr6.4:47578-47597	MS.gene74452:CDS	35.0%
	AAGAGTCTTAAGAGAATGCT+TGG	-	chr6.4:47569-47588	MS.gene74452:CDS	35.0%
	ACATCTTAAAAGCTTCCCTT+AGG	+	chr6.4:47517-47536	None:intergenic	35.0%
	AGAGAAAGTGAATGACCTAA+GGG	-	chr6.4:47499-47518	MS.gene74452:CDS	35.0%
	AGATGGAAAAATATCACCGT+CGG	-	chr6.4:47334-47353	MS.gene74452:CDS	35.0%
	AGCATTCTCTTAAGACTCTT+TGG	+	chr6.4:47569-47588	None:intergenic	35.0%
	ATTTGACATTGATGGAGATG+GGG	-	chr6.4:47643-47662	MS.gene74452:CDS	35.0%
	CAATTTGACATTGATGGAGA+TGG	-	chr6.4:47641-47660	MS.gene74452:CDS	35.0%
	CACTTTGATGAAAATGGAGA+TGG	-	chr6.4:47317-47336	MS.gene74452:CDS	35.0%
	TAAGAGAATGCTTGGAAGAT+TGG	-	chr6.4:47577-47596	MS.gene74452:CDS	35.0%
!	CATCTCCATTTTCATCAAAG+TGG	+	chr6.4:47319-47338	None:intergenic	35.0%
	TTGTGAAGTTTGTAGAGGGA+GGG	-	chr6.4:47468-47487	MS.gene74452:CDS	40.0%
	TTTGTGAAGTTTGTAGAGGG+AGG	-	chr6.4:47467-47486	MS.gene74452:CDS	40.0%
	ATGTATGAAATGGAGGGGTG+TGG	-	chr6.4:47533-47552	MS.gene74452:CDS	45.0%
	AAATATCACCGTCGGAGCTG+CGG	-	chr6.4:47342-47361	MS.gene74452:CDS	50.0%
	GTTTGTAGAGGGAGGGAAAG+AGG	-	chr6.4:47475-47494	MS.gene74452:CDS	50.0%
	CAAGATGTCGAACGAGGAGG+CGG	-	chr6.4:47388-47407	MS.gene74452:CDS	55.0%
	CGGCAATGTGTGGAGGCAAT+CGG	-	chr6.4:47362-47381	MS.gene74452:CDS	55.0%
	GCAATGTGTGGAGGCAATCG+GGG	-	chr6.4:47364-47383	MS.gene74452:CDS	55.0%
	GGCAATGTGTGGAGGCAATC+GGG	-	chr6.4:47363-47382	MS.gene74452:CDS	55.0%
!!	GTTGAGCTTCTCGACTCGGA+CGG	-	chr6.4:47419-47438	MS.gene74452:CDS	55.0%
!!	TTGAGCTTCTCGACTCGGAC+GGG	-	chr6.4:47420-47439	MS.gene74452:CDS	55.0%
	ACACATTGCCGCAGCTCCGA+CGG	+	chr6.4:47353-47372	None:intergenic	60.0%
	CGCCTCCTCGTTCGACATCT+TGG	+	chr6.4:47390-47409	None:intergenic	60.0%
	GAACGAGGAGGCGGAAATGG+CGG	-	chr6.4:47397-47416	MS.gene74452:CDS	60.0%
	GGCCAAGATGTCGAACGAGG+AGG	-	chr6.4:47385-47404	MS.gene74452:CDS	60.0%
	GGCGGTTGAGCTTCTCGACT+CGG	-	chr6.4:47415-47434	MS.gene74452:CDS	60.0%
	GTCGAACGAGGAGGCGGAAA+TGG	-	chr6.4:47394-47413	MS.gene74452:CDS	60.0%
!!	CGGACGGGGATGGGTTAGTT+GGG	-	chr6.4:47435-47454	MS.gene74452:CDS	60.0%
!!	CGGGGATGGGTTAGTTGGGT+TGG	-	chr6.4:47439-47458	MS.gene74452:CDS	60.0%
!!	TCGGACGGGGATGGGTTAGT+TGG	-	chr6.4:47434-47453	MS.gene74452:CDS	60.0%
!!	TGAGCTTCTCGACTCGGACG+GGG	-	chr6.4:47421-47440	MS.gene74452:CDS	60.0%
!!	TTCTCGACTCGGACGGGGAT+GGG	-	chr6.4:47426-47445	MS.gene74452:CDS	60.0%
	CGGGGCCAAGATGTCGAACG+AGG	-	chr6.4:47382-47401	MS.gene74452:CDS	65.0%
	GGAGCTGCGGCAATGTGTGG+AGG	-	chr6.4:47355-47374	MS.gene74452:CDS	65.0%
	GTCGGAGCTGCGGCAATGTG+TGG	-	chr6.4:47352-47371	MS.gene74452:CDS	65.0%
!!	CTTCTCGACTCGGACGGGGA+TGG	-	chr6.4:47425-47444	MS.gene74452:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	47275	47703	47275	ID=MS.gene74452
chr6.4	mRNA	47275	47703	47275	ID=MS.gene74452.t1;Parent=MS.gene74452
chr6.4	exon	47275	47703	47275	ID=MS.gene74452.t1.exon1;Parent=MS.gene74452.t1
chr6.4	CDS	47275	47703	47275	ID=cds.MS.gene74452.t1;Parent=MS.gene74452.t1

Gene Sequence

>MS.gene74452

ATGGTCATGGACAAGGTGACTCAATACATACGTGTGTTCAACCACTTTGATGAAAATGGAGATGGAAAAATATCACCGTCGGAGCTGCGGCAATGTGTGGAGGCAATCGGGGCCAAGATGTCGAACGAGGAGGCGGAAATGGCGGTTGAGCTTCTCGACTCGGACGGGGATGGGTTAGTTGGGTTGGATGATTTTGTGAAGTTTGTAGAGGGAGGGAAAGAGGAAGAGAAAGTGAATGACCTAAGGGAAGCTTTTAAGATGTATGAAATGGAGGGGTGTGGTTGCATCACACCAAAGAGTCTTAAGAGAATGCTTGGAAGATTGGGTGAGTGTAGAAGTGTAGATGAATGTCAAGCTATGATTTCTCAATTTGACATTGATGGAGATGGGGTCCTTAGTTTTGATGAATTTAGGACAATGATGCTTTAA

Protein sequence

>MS.gene74452.t1

MVMDKVTQYIRVFNHFDENGDGKISPSELRQCVEAIGAKMSNEEAEMAVELLDSDGDGLVGLDDFVKFVEGGKEEEKVNDLREAFKMYEMEGCGCITPKSLKRMLGRLGECRSVDECQAMISQFDIDGDGVLSFDEFRTMML