AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95112


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95112.t1 MTR_4g133870 86.364 352 11 1 1 315 1 352 0.0 597
MS.gene95112.t1 MTR_5g095650 48.991 347 136 3 1 310 1 343 8.61e-98 292
MS.gene95112.t1 MTR_7g110600 35.417 240 149 3 71 306 136 373 2.59e-48 166
MS.gene95112.t1 MTR_5g091565 37.705 244 145 3 71 310 139 379 5.50e-48 165
MS.gene95112.t1 MTR_4g094582 35.510 245 148 5 71 310 174 413 1.82e-46 162
MS.gene95112.t1 MTR_4g133820 62.069 58 21 1 66 123 53 109 1.56e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95112.t1 AT5G55630 59.292 226 92 0 85 310 127 352 1.57e-92 279
MS.gene95112.t1 AT5G55630 59.292 226 92 0 85 310 127 352 1.57e-92 279
MS.gene95112.t1 AT4G18160 37.295 244 146 4 71 310 189 429 1.80e-45 160
MS.gene95112.t1 AT4G01840 33.600 250 154 3 71 310 156 403 1.34e-42 152
MS.gene95112.t1 AT5G46370 33.871 248 153 4 71 310 185 429 3.11e-42 151
MS.gene95112.t1 AT1G02510 32.174 230 132 4 69 298 72 277 1.23e-31 120
MS.gene95112.t1 AT5G46360 40.268 149 89 0 162 310 106 254 3.15e-31 118
MS.gene95112.t1 AT5G46360 38.732 142 86 1 146 286 89 230 4.58e-26 104

Find 47 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCCACGGTCACTACTTT+AGG 0.301171 8.2:-1000607 MS.gene95112:CDS
GTTATACATTGTGGAGATAA+AGG 0.305350 8.2:+1001120 None:intergenic
ATGTGCAAGGCTGAAAATTT+TGG 0.309852 8.2:-1000781 MS.gene95112:CDS
TGTATACAAGTTGAAGGAAA+TGG 0.323817 8.2:-999785 MS.gene95112:CDS
CGGTCACTACTTTAGGTTAT+GGG 0.344172 8.2:-1000600 MS.gene95112:CDS
TCACTGCTGAAATATCTTCT+TGG 0.344460 8.2:+999752 None:intergenic
AGATTACATTGTTGAGAAAC+AGG 0.387285 8.2:-1000824 MS.gene95112:CDS
ACGGTCACTACTTTAGGTTA+TGG 0.387375 8.2:-1000601 MS.gene95112:CDS
TTATACATTGTGGAGATAAA+GGG 0.395633 8.2:+1001121 None:intergenic
ATTGTGGAGATAAAGGGGTT+TGG 0.406546 8.2:+1001127 None:intergenic
GTATACAAGTTGAAGGAAAT+GGG 0.421596 8.2:-999784 MS.gene95112:CDS
CTTGAGGCAGCAGATCTTGA+TGG 0.425392 8.2:-1000409 MS.gene95112:CDS
GTCTTCACTGGCATGGCTCT+CGG 0.434853 8.2:-1000871 MS.gene95112:CDS
GTTTGTTGTATACAAGTTGA+AGG 0.439684 8.2:-999791 MS.gene95112:CDS
GCATGCATTTATGTCTTCAC+TGG 0.440926 8.2:-1000883 MS.gene95112:CDS
ATTGAGCAGCACCATTTGCT+TGG 0.490697 8.2:-1000527 MS.gene95112:CDS
AGCTATTGCTTTCAGAGTCA+AGG 0.495133 8.2:-1001260 MS.gene95112:CDS
GTGGAGATAAAGGGGTTTGG+AGG 0.501419 8.2:+1001130 None:intergenic
AAACTAGACTGTGATCAATC+AGG 0.508619 8.2:-999715 MS.gene95112:CDS
CATTTATGTCTTCACTGGCA+TGG 0.510032 8.2:-1000878 MS.gene95112:CDS
AGAAGATATTTCAGCAGTGA+TGG 0.521751 8.2:-999749 MS.gene95112:CDS
AAGAGCTTCTCGACTGCTGC+CGG 0.529816 8.2:-1000574 MS.gene95112:CDS
AAGAAGCAGAATTCAAGTTC+AGG 0.531566 8.2:-1001089 MS.gene95112:CDS
AAAAGACTGAGCCAAGCAAA+TGG 0.534462 8.2:+1000516 None:intergenic
GCGGTCTTCTTCTCTGAAGA+AGG 0.536019 8.2:+1001208 None:intergenic
ACTCTATTGTGTTTGTTCCA+CGG 0.539533 8.2:-1000620 MS.gene95112:CDS
TGTTTATCAGTTATACATTG+TGG 0.539721 8.2:+1001111 None:intergenic
TTGATGGAGACAAAGTTGTC+AGG 0.546814 8.2:-1000393 MS.gene95112:intron
GCTATTGCTTTCAGAGTCAA+GGG 0.570375 8.2:-1001259 MS.gene95112:CDS
TCTTGCTGAGTCCATGTGCA+AGG 0.575638 8.2:-1000794 MS.gene95112:CDS
ATTATCACTAAGTGATCTTG+AGG 0.579141 8.2:-1000425 MS.gene95112:CDS
TATACATTGTGGAGATAAAG+GGG 0.579592 8.2:+1001122 None:intergenic
TGAAGACATAAATGCATGCA+AGG 0.589047 8.2:+1000887 None:intergenic
TTGTGTTTAGCAGCATACCT+AGG 0.589326 8.2:-1001057 MS.gene95112:CDS
GGTCACTACTTTAGGTTATG+GGG 0.598779 8.2:-1000599 MS.gene95112:CDS
TGAAGATAGACAAAGATCAC+TGG 0.601013 8.2:-1000470 MS.gene95112:CDS
AACATAAGGTACCTGTGCCT+AGG 0.617422 8.2:+1001040 None:intergenic
AGAAGAAGACCGCGGCGTGG+CGG 0.622978 8.2:-1001198 MS.gene95112:CDS
GCTTCTCGACTGCTGCCGGT+AGG 0.626037 8.2:-1000570 MS.gene95112:CDS
TTAGCAGCATACCTAGGCAC+AGG 0.645513 8.2:-1001051 MS.gene95112:intron
TTCACTGGCATGGCTCTCGG+TGG 0.647484 8.2:-1000868 MS.gene95112:CDS
TCTGAAGTTCCGCCACGCCG+CGG 0.651177 8.2:+1001189 None:intergenic
AATCTGCAGCAAGAACAGAA+TGG 0.667031 8.2:-1001156 MS.gene95112:CDS
CAGAGAAGAAGACCGCGGCG+TGG 0.691942 8.2:-1001201 MS.gene95112:CDS
GCTGCTCAATATCCAAAACA+CGG 0.699934 8.2:+1000540 None:intergenic
ATAACCTAAAGTAGTGACCG+TGG 0.717695 8.2:+1000603 None:intergenic
TTCTTCAGAGAAGAAGACCG+CGG 0.758085 8.2:-1001206 MS.gene95112:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAATAAAATAAAA+TGG - chr8.2:1000792-1000811 MS.gene95112:CDS 0.0%
!!! ATTTTTTAATTATTTTTTTT+TGG - chr8.2:1000903-1000922 MS.gene95112:CDS 0.0%
!! ATAAAATGGAAATTAAAATA+AGG - chr8.2:1000806-1000825 MS.gene95112:CDS 10.0%
!! AAAATTGTTGATCAATATTG+AGG + chr8.2:1001130-1001149 None:intergenic 20.0%
!! TTATGAGTGAATTTGTAAAT+TGG + chr8.2:1000706-1000725 None:intergenic 20.0%
!! TTTATTTATATCATTTGCAC+CGG - chr8.2:1001013-1001032 MS.gene95112:intron 20.0%
! TGTTTATCAGTTATACATTG+TGG + chr8.2:999849-999868 None:intergenic 25.0%
! TTATACATTGTGGAGATAAA+GGG + chr8.2:999839-999858 None:intergenic 25.0%
! TTATTTATATCATTTGCACC+GGG - chr8.2:1001014-1001033 MS.gene95112:intron 25.0%
!! ACTTGTTAGATAGAAACATA+AGG + chr8.2:999934-999953 None:intergenic 25.0%
!! ATCACTTAGTGATAATTTTC+GGG + chr8.2:1000528-1000547 None:intergenic 25.0%
!! GATCACTTAGTGATAATTTT+CGG + chr8.2:1000529-1000548 None:intergenic 25.0%
!! TATTGAGAATCTAGATTTTG+TGG - chr8.2:1000310-1000329 MS.gene95112:intron 25.0%
AATCATGAGCACAAAAAAAG+TGG + chr8.2:1000267-1000286 None:intergenic 30.0%
AATCTCTGATTATTGCGAAT+TGG + chr8.2:1000654-1000673 None:intergenic 30.0%
AGATTACATTGTTGAGAAAC+AGG - chr8.2:1000133-1000152 MS.gene95112:intron 30.0%
ATTATCACTAAGTGATCTTG+AGG - chr8.2:1000532-1000551 MS.gene95112:CDS 30.0%
CTAACAAGTTACCAAATTGA+GGG - chr8.2:999945-999964 MS.gene95112:intron 30.0%
GCTTAGTGTAGCTAAATTAT+TGG + chr8.2:1001099-1001118 None:intergenic 30.0%
GTTATACATTGTGGAGATAA+AGG + chr8.2:999840-999859 None:intergenic 30.0%
TATACATTGTGGAGATAAAG+GGG + chr8.2:999838-999857 None:intergenic 30.0%
TCTAACAAGTTACCAAATTG+AGG - chr8.2:999944-999963 MS.gene95112:intron 30.0%
TGTCACAACACAGAAATAAA+GGG + chr8.2:999997-1000016 None:intergenic 30.0%
TTGTCACAACACAGAAATAA+AGG + chr8.2:999998-1000017 None:intergenic 30.0%
! GTATACAAGTTGAAGGAAAT+GGG - chr8.2:1001173-1001192 MS.gene95112:CDS 30.0%
! TGTATACAAGTTGAAGGAAA+TGG - chr8.2:1001172-1001191 MS.gene95112:CDS 30.0%
!! AGTTTTGCTATAGTGCTATT+TGG + chr8.2:1000050-1000069 None:intergenic 30.0%
!! GTTTGTTGTATACAAGTTGA+AGG - chr8.2:1001166-1001185 MS.gene95112:CDS 30.0%
!! TGAAAATTTTGGTCTACAAG+AGG - chr8.2:1000187-1000206 MS.gene95112:intron 30.0%
!!! TTGTTTTTATGCCCTCAATT+TGG + chr8.2:999959-999978 None:intergenic 30.0%
AAACTAGACTGTGATCAATC+AGG - chr8.2:1001242-1001261 MS.gene95112:CDS 35.0%
ACAAAGATCACTGGCAAAAA+TGG - chr8.2:1000496-1000515 MS.gene95112:CDS 35.0%
ACAATGACTACTGTTGGATA+TGG - chr8.2:1000014-1000033 MS.gene95112:intron 35.0%
ACTCCTTTGATATGTATCGA+CGG - chr8.2:1000928-1000947 MS.gene95112:intron 35.0%
ACTCTATTGTGTTTGTTCCA+CGG - chr8.2:1000337-1000356 MS.gene95112:intron 35.0%
AGAAGATATTTCAGCAGTGA+TGG - chr8.2:1001208-1001227 MS.gene95112:CDS 35.0%
CACTCATAAGTTATGAAGCA+CGG - chr8.2:1000717-1000736 MS.gene95112:CDS 35.0%
TAAACAATCACACGTATCTG+AGG - chr8.2:1000836-1000855 MS.gene95112:CDS 35.0%
TCACTGCTGAAATATCTTCT+TGG + chr8.2:1001208-1001227 None:intergenic 35.0%
TGAAGACATAAATGCATGCA+AGG + chr8.2:1000073-1000092 None:intergenic 35.0%
TGAAGATAGACAAAGATCAC+TGG - chr8.2:1000487-1000506 MS.gene95112:CDS 35.0%
! AAAATTTTCAGCCTTGCACA+TGG + chr8.2:1000177-1000196 None:intergenic 35.0%
! AAGAAGCAGAATTCAAGTTC+AGG - chr8.2:999868-999887 MS.gene95112:intron 35.0%
! ATGTGCAAGGCTGAAAATTT+TGG - chr8.2:1000176-1000195 MS.gene95112:intron 35.0%
!!! TTTTTTGTGCTCATGATTGC+AGG - chr8.2:1000269-1000288 MS.gene95112:intron 35.0%
AAAAGACTGAGCCAAGCAAA+TGG + chr8.2:1000444-1000463 None:intergenic 40.0%
AAACACGGCAAAAATCCTAC+CGG + chr8.2:1000405-1000424 None:intergenic 40.0%
AATCTGCAGCAAGAACAGAA+TGG - chr8.2:999801-999820 MS.gene95112:CDS 40.0%
AGCTATTGCTTTCAGAGTCA+AGG - chr8.2:999697-999716 MS.gene95112:CDS 40.0%
ATAACCTAAAGTAGTGACCG+TGG + chr8.2:1000357-1000376 None:intergenic 40.0%
ATTGTGGAGATAAAGGGGTT+TGG + chr8.2:999833-999852 None:intergenic 40.0%
CATTTATGTCTTCACTGGCA+TGG - chr8.2:1000079-1000098 MS.gene95112:intron 40.0%
GCATGCATTTATGTCTTCAC+TGG - chr8.2:1000074-1000093 MS.gene95112:intron 40.0%
GCTATTGCTTTCAGAGTCAA+GGG - chr8.2:999698-999717 MS.gene95112:CDS 40.0%
GCTGCTCAATATCCAAAACA+CGG + chr8.2:1000420-1000439 None:intergenic 40.0%
GTATCAGATACACGTATCGA+CGG + chr8.2:1000751-1000770 None:intergenic 40.0%
GTTGTGACAATGACTACTGT+TGG - chr8.2:1000008-1000027 MS.gene95112:intron 40.0%
TATGATCTTACTGCATCTCC+CGG + chr8.2:1001035-1001054 None:intergenic 40.0%
TCAACGCAATACTAGTGATG+AGG + chr8.2:1001067-1001086 None:intergenic 40.0%
TTGATGGAGACAAAGTTGTC+AGG - chr8.2:1000564-1000583 MS.gene95112:CDS 40.0%
TTGTGTTTAGCAGCATACCT+AGG - chr8.2:999900-999919 MS.gene95112:intron 40.0%
! ACGGTCACTACTTTAGGTTA+TGG - chr8.2:1000356-1000375 MS.gene95112:intron 40.0%
! CGGTCACTACTTTAGGTTAT+GGG - chr8.2:1000357-1000376 MS.gene95112:intron 40.0%
! GGTCACTACTTTAGGTTATG+GGG - chr8.2:1000358-1000377 MS.gene95112:intron 40.0%
! GTAGGATTTTTGCCGTGTTT+TGG - chr8.2:1000405-1000424 MS.gene95112:CDS 40.0%
ACAACAAACTCTGCAGCACT+GGG + chr8.2:1001155-1001174 None:intergenic 45.0%
ACGCCGTCGATACATATCAA+AGG + chr8.2:1000934-1000953 None:intergenic 45.0%
AGCAGACACTTCACAATTGC+AGG - chr8.2:1000618-1000637 MS.gene95112:CDS 45.0%
ATTGAGCAGCACCATTTGCT+TGG - chr8.2:1000430-1000449 MS.gene95112:CDS 45.0%
GCAGACACTTCACAATTGCA+GGG - chr8.2:1000619-1000638 MS.gene95112:CDS 45.0%
TACAACAAACTCTGCAGCAC+TGG + chr8.2:1001156-1001175 None:intergenic 45.0%
TGTTCCACGGTCACTACTTT+AGG - chr8.2:1000350-1000369 MS.gene95112:intron 45.0%
TTCTTCAGAGAAGAAGACCG+CGG - chr8.2:999751-999770 MS.gene95112:CDS 45.0%
! AACATAAGGTACCTGTGCCT+AGG + chr8.2:999920-999939 None:intergenic 45.0%
CTTGAGGCAGCAGATCTTGA+TGG - chr8.2:1000548-1000567 MS.gene95112:CDS 50.0%
GTGGAGATAAAGGGGTTTGG+AGG + chr8.2:999830-999849 None:intergenic 50.0%
TCTTGCTGAGTCCATGTGCA+AGG - chr8.2:1000163-1000182 MS.gene95112:intron 50.0%
TTAGCAGCATACCTAGGCAC+AGG - chr8.2:999906-999925 MS.gene95112:intron 50.0%
! GCGGTCTTCTTCTCTGAAGA+AGG + chr8.2:999752-999771 None:intergenic 50.0%
AAGAGCTTCTCGACTGCTGC+CGG - chr8.2:1000383-1000402 MS.gene95112:intron 55.0%
GTCTTCACTGGCATGGCTCT+CGG - chr8.2:1000086-1000105 MS.gene95112:intron 55.0%
TTCACTGGCATGGCTCTCGG+TGG - chr8.2:1000089-1000108 MS.gene95112:intron 60.0%
! CACACGTATCTGAGGCGTGC+TGG - chr8.2:1000844-1000863 MS.gene95112:CDS 60.0%
! GAGACGTGTCGACGGCGTAT+CGG - chr8.2:1000866-1000885 MS.gene95112:CDS 60.0%
AGAAGAAGACCGCGGCGTGG+CGG - chr8.2:999759-999778 MS.gene95112:CDS 65.0%
CAGAGAAGAAGACCGCGGCG+TGG - chr8.2:999756-999775 MS.gene95112:CDS 65.0%
GCGTGCTGGAGACGTGTCGA+CGG - chr8.2:1000858-1000877 MS.gene95112:CDS 65.0%
GCTTCTCGACTGCTGCCGGT+AGG - chr8.2:1000387-1000406 MS.gene95112:intron 65.0%
! TCTGAAGTTCCGCCACGCCG+CGG + chr8.2:999771-999790 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 999675 1001304 999675 ID=MS.gene95112
chr8.2 mRNA 999675 1001304 999675 ID=MS.gene95112.t1;Parent=MS.gene95112
chr8.2 exon 1001052 1001304 1001052 ID=MS.gene95112.t1.exon1;Parent=MS.gene95112.t1
chr8.2 CDS 1001052 1001304 1001052 ID=cds.MS.gene95112.t1;Parent=MS.gene95112.t1
chr8.2 exon 1000394 1000940 1000394 ID=MS.gene95112.t1.exon2;Parent=MS.gene95112.t1
chr8.2 CDS 1000394 1000940 1000394 ID=cds.MS.gene95112.t1;Parent=MS.gene95112.t1
chr8.2 exon 999675 999822 999675 ID=MS.gene95112.t1.exon3;Parent=MS.gene95112.t1
chr8.2 CDS 999675 999822 999675 ID=cds.MS.gene95112.t1;Parent=MS.gene95112.t1
Gene Sequence

>MS.gene95112

ATGGGCAGTGATGAAGCTCAACAGCTATTGCTTTCAGAGTCAAGGGAACATTCTCATCTCAATGAGAAGAGTGACCTTCTTCAGAGAAGAAGACCGCGGCGTGGCGGAACTTCAGAGACAGATATCAATCTGCAGCAAGAACAGAATGGTGTCCTCCAAACCCCTTTATCTCCACAATGTATAACTGATAAACAAGAAGCAGAATTCAAGTTCAGGCTAGTGTTCTTGTGTTTAGCAGCATACCTAGGCACAGGTACCTTATGTTTCTATCTAACAAGTTACCAAATTGAGGGCATAAAAACAAATAATTTTCTTGATGCCCTTTATTTCTGTGTTGTGACAATGACTACTGTTGGATATGGAGATCTTGTTCCAAATAGCACTATAGCAAAACTCCTTGCATGCATTTATGTCTTCACTGGCATGGCTCTCGGTGGCTTAATTCTTAGCAAAGCAGCAGATTACATTGTTGAGAAACAGGAAATATTTCTTGCTGAGTCCATGTGCAAGGCTGAAAATTTTGGTCTACAAGAGGTTGCTAAAGAGCTTGAAACTAAGAAAAGCAAATACAAGTTTGTGCTTGCAGTAGCCACTTTTTTTGTGCTCATGATTGCAGGAACTGTTTTTCTGTATTTTATTGAGAATCTAGATTTTGTGGATGCACTCTATTGTGTTTGTTCCACGGTCACTACTTTAGGTTATGGGGATAAGAGCTTCTCGACTGCTGCCGGTAGGATTTTTGCCGTGTTTTGGATATTGAGCAGCACCATTTGCTTGGCTCAGTCTTTTGCTTATCTTGCTGAACTCTATACTGAAGATAGACAAAGATCACTGGCAAAAATGGTTCTCACCCGAAAATTATCACTAAGTGATCTTGAGGCAGCAGATCTTGATGGAGACAAAGTTGTCAGGTACTAAAATACACTAACAGATGAACTATTTTAGCAGACACTTCACAATTGCAGGGATATATTTTCCAATTCGCAATAATCAGAGATTATTTTGACTGATGCTTATAATTTCGAGAACCAATTTACAAATTCACTCATAAGTTATGAAGCACGGACACGATACCGTCGATACGTGTATCTGATACGATACGTTTAAGATACATTTATAAAAAAAAATAAAATAAAATGGAAATTAAAATAAGGAGAGAGCTAAACAATCACACGTATCTGAGGCGTGCTGGAGACGTGTCGACGGCGTATCGGAATTTTTTATTTTTATTTTTTAATTATTTTTTTTTGGATACTCCTTTGATATGTATCGACGGCGTATCAGATACGTATCAGTGCTTCATAGTGCTAAGCTTATATTATAAATGTATTTACTCTTTTTATTTATATCATTTGCACCGGGAGATGCAGTAAGATCATATTTATAACACCTCATCACTAGTATTGCGTTGATAAATTGCTCCAATAATTTAGCTACACTAAGCAATTAAATCCTCAATATTGATCAACAATTTTTTCCCAGTGCTGCAGAGTTTGTTGTATACAAGTTGAAGGAAATGGGAAAGATCAACCAAGAAGATATTTCAGCAGTGATGGAGAGTTTTAGAAAACTAGACTGTGATCAATCAGGAACTCTGACTGAAGCTGATATCAGAAATTTTGAATTCTGA

Protein sequence

>MS.gene95112.t1

MGSDEAQQLLLSESREHSHLNEKSDLLQRRRPRRGGTSETDINLQQEQNGVLQTPLSPQCITDKQEAEFKFRLVFLCLAAYLGTDLVPNSTIAKLLACIYVFTGMALGGLILSKAADYIVEKQEIFLAESMCKAENFGLQEVAKELETKKSKYKFVLAVATFFVLMIAGTVFLYFIENLDFVDALYCVCSTVTTLGYGDKSFSTAAGRIFAVFWILSSTICLAQSFAYLAELYTEDRQRSLAKMVLTRKLSLSDLEAADLDGDKVVSAAEFVVYKLKEMGKINQEDISAVMESFRKLDCDQSGTLTEADIRNFEF