AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95614


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95614.t1 MTR_7g068770 97.279 294 6 2 1 293 1 293 0.0 575
MS.gene95614.t1 MTR_4g015680 76.984 252 57 1 41 291 28 279 6.21e-119 342
MS.gene95614.t1 MTR_5g026540 46.619 281 123 8 37 292 23 301 1.89e-56 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95614.t1 AT5G01380 55.056 267 96 3 49 291 47 313 1.14e-74 231
MS.gene95614.t1 AT2G38250 57.299 274 96 4 33 291 19 286 3.17e-74 229
MS.gene95614.t1 AT3G25990 31.858 113 74 1 54 163 55 167 6.03e-14 71.6
MS.gene95614.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.10e-11 64.3
MS.gene95614.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.10e-11 64.3
MS.gene95614.t1 AT1G13450 33.696 92 61 0 54 145 87 178 1.89e-11 64.3
MS.gene95614.t1 AT1G13450 33.696 92 61 0 54 145 87 178 2.98e-11 62.4

Find 61 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGAAACTCCTTTGTTTCT+TGG 0.162176 7.4:+34769370 None:intergenic
AGAACTAGATCAAACTTTCA+TGG 0.325286 7.4:-34769337 MS.gene95614:CDS
GCTTGAAGCTCATTGTAAAA+TGG 0.335464 7.4:+34768655 None:intergenic
AAACCTTGTTACTCGCTATA+AGG 0.341215 7.4:-34769217 MS.gene95614:intron
GTTGATGTTGTTGATGATGT+TGG 0.399503 7.4:+34769520 None:intergenic
CAAAGAGGAACAAACAGCTT+TGG 0.409902 7.4:-34769311 MS.gene95614:CDS
TGAGAGGAGATTGAAGGAGA+TGG 0.420471 7.4:-34768344 MS.gene95614:CDS
AAAGAGGAACAAACAGCTTT+GGG 0.427051 7.4:-34769310 MS.gene95614:CDS
CTCTAACACTATGAAGGAAA+AGG 0.429380 7.4:-34769280 MS.gene95614:CDS
CAACATGCTCATAACATCAT+AGG 0.432593 7.4:-34769444 MS.gene95614:CDS
TGGAACAAAGATGGAGAGAA+AGG 0.438127 7.4:-34768276 MS.gene95614:CDS
AGATTCTTGAGGAGTTTATG+AGG 0.459685 7.4:-34768420 MS.gene95614:CDS
GGAGATTGAAGGAGATGGAG+TGG 0.466184 7.4:-34768339 MS.gene95614:CDS
TCTAACACTATGAAGGAAAA+GGG 0.470472 7.4:-34769279 MS.gene95614:CDS
GAAGGATTAGAGAAGAAGCT+AGG 0.478364 7.4:-34768243 MS.gene95614:CDS
TCAATGGATGGAAGCATTTG+AGG 0.478413 7.4:-34768377 MS.gene95614:CDS
TCTGAGGATGAAGAAGAGTT+AGG 0.481694 7.4:-34768553 MS.gene95614:CDS
AAGGAGGGTCAAAGAAGAAA+GGG 0.488338 7.4:-34768588 MS.gene95614:CDS
GATGGAGTGGAGACAAAGAA+TGG 0.488486 7.4:-34768326 MS.gene95614:CDS
GGAGAATGAGAGGAGATTGA+AGG 0.494385 7.4:-34768350 MS.gene95614:CDS
GGTTAATGATGGAACAAAGA+TGG 0.500019 7.4:-34768285 MS.gene95614:CDS
GTTAGGCAATGAAGAAAGTG+AGG 0.500463 7.4:-34768536 MS.gene95614:CDS
GAAGGAGGGTCAAAGAAGAA+AGG 0.513151 7.4:-34768589 MS.gene95614:CDS
GAGACAAAGAATGGAAGCAT+TGG 0.518130 7.4:-34768317 MS.gene95614:CDS
CAAGGATGCAAAGAATGCTA+TGG 0.520600 7.4:-34768621 MS.gene95614:CDS
GAGAAAGGGAAGAACAAAGA+AGG 0.520747 7.4:-34768261 MS.gene95614:CDS
TATGAGGCAGCAAATGCAAA+TGG 0.520773 7.4:-34768404 MS.gene95614:CDS
GGAACAAAGATGGAGAGAAA+GGG 0.522554 7.4:-34768275 MS.gene95614:CDS
TAGTGGTAGCAACAATTTGA+AGG 0.523300 7.4:-34768443 MS.gene95614:CDS
AGTTATCTCTAACACTATGA+AGG 0.525230 7.4:-34769286 MS.gene95614:CDS
TCACGGATAGATTTCCTCAA+TGG 0.529342 7.4:-34769398 MS.gene95614:CDS
GGTGGAGGAAATGATAATAG+TGG 0.531218 7.4:-34768460 MS.gene95614:CDS
TGGAAGCATTGGAGAATGAG+AGG 0.532379 7.4:-34768306 MS.gene95614:CDS
GGAGAATGAGAGGTTAATGA+TGG 0.535043 7.4:-34768296 MS.gene95614:CDS
CAGAACAGTGCAAGTGCAAG+TGG 0.545159 7.4:-34769242 MS.gene95614:CDS
CAATTTGAAGGAGATTCTTG+AGG 0.551112 7.4:-34768431 MS.gene95614:CDS
AAATGGAAATTGTTGTCTCA+TGG 0.560448 7.4:+34768672 None:intergenic
AAATGCAAATGGAAGCTCAA+TGG 0.561080 7.4:-34768393 MS.gene95614:CDS
AAAGGTAAGATGATAATTGG+TGG 0.562069 7.4:-34768478 MS.gene95614:CDS
TAGTGTTAACGTTGATGTCA+CGG 0.564347 7.4:-34769415 MS.gene95614:CDS
AAGGATTAGAGAAGAAGCTA+GGG 0.564945 7.4:-34768242 MS.gene95614:CDS
GTTTCTTGGATACTCCATTG+AGG 0.570470 7.4:+34769384 None:intergenic
ATGCTATGGGCTGAAGCAGA+AGG 0.573045 7.4:-34768607 MS.gene95614:CDS
GGATGGAAGCATTTGAGGCA+AGG 0.573712 7.4:-34768372 MS.gene95614:CDS
TTGAGGCAAGGGAGAATGAG+AGG 0.584023 7.4:-34768360 MS.gene95614:CDS
GCAAATGGAAGCTCAATGGA+TGG 0.584747 7.4:-34768389 MS.gene95614:CDS
GATGGAAGCATTTGAGGCAA+GGG 0.587229 7.4:-34768371 MS.gene95614:CDS
AGAAAGTGAGGGAGATCACA+AGG 0.594638 7.4:-34768524 MS.gene95614:CDS
TTAGGCAATGAAGAAAGTGA+GGG 0.597635 7.4:-34768535 MS.gene95614:CDS
GGTAAGATGATAATTGGTGG+AGG 0.599324 7.4:-34768475 MS.gene95614:CDS
CTATGGGCTGAAGCAGAAGG+AGG 0.599474 7.4:-34768604 MS.gene95614:CDS
AAACTTTCATGGAGACAAAG+AGG 0.604350 7.4:-34769326 MS.gene95614:CDS
TATAAAGGGATGTGAAACAA+TGG 0.607384 7.4:-34768704 MS.gene95614:intron
ATGGAGTATCCAAGAAACAA+AGG 0.617542 7.4:-34769379 MS.gene95614:CDS
AGGGGTGCATGTATCTTCTG+AGG 0.631436 7.4:-34768569 MS.gene95614:CDS
AAGTTTGATCTAGTTCTGAA+CGG 0.639250 7.4:+34769343 None:intergenic
AAGGATGCAAAGAATGCTAT+GGG 0.642173 7.4:-34768620 MS.gene95614:CDS
ATACCTTATAGCGAGTAACA+AGG 0.647395 7.4:+34769214 None:intergenic
AGGAGGGTCAAAGAAGAAAG+GGG 0.670389 7.4:-34768587 MS.gene95614:CDS
TATGGGCTGAAGCAGAAGGA+GGG 0.673793 7.4:-34768603 MS.gene95614:CDS
GAAAGTGAGGGAGATCACAA+GGG 0.730407 7.4:-34768523 MS.gene95614:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTAAACTTTTTTTT+TGG - chr7.4:34768718-34768737 MS.gene95614:intron 10.0%
!! ATTAAAAAGAAGAAGAAAAA+AGG - chr7.4:34769221-34769240 MS.gene95614:CDS 15.0%
!!! ATTGTTTTGTTTATGTTTTT+GGG - chr7.4:34768989-34769008 MS.gene95614:intron 15.0%
!!! TATTGTTTTGTTTATGTTTT+TGG - chr7.4:34768988-34769007 MS.gene95614:intron 15.0%
!! AAAAAAGGTAAGATGATAAT+TGG - chr7.4:34769236-34769255 MS.gene95614:CDS 20.0%
!! AAAAGGCAATAAATAAAAAG+TGG + chr7.4:34768796-34768815 None:intergenic 20.0%
!! AAACAAGAATGATTTATGAT+TGG + chr7.4:34768640-34768659 None:intergenic 20.0%
!!! TTATGTTTTTGGGATATAAA+GGG - chr7.4:34768999-34769018 MS.gene95614:intron 20.0%
!!! TTTATGTTTTTGGGATATAA+AGG - chr7.4:34768998-34769017 MS.gene95614:intron 20.0%
! CACATAGAATGATTTATGAT+TGG + chr7.4:34768581-34768600 None:intergenic 25.0%
!! TTTATATTGTGAGCTTTTCA+TGG - chr7.4:34768936-34768955 MS.gene95614:intron 25.0%
!!! ATTGCCTTTTCAATTTGTTA+GGG - chr7.4:34768806-34768825 MS.gene95614:intron 25.0%
!!! TATTGCCTTTTCAATTTGTT+AGG - chr7.4:34768805-34768824 MS.gene95614:intron 25.0%
AAAGGTAAGATGATAATTGG+TGG - chr7.4:34769239-34769258 MS.gene95614:CDS 30.0%
AAATGGAAATTGTTGTCTCA+TGG + chr7.4:34769048-34769067 None:intergenic 30.0%
AGAACTAGATCAAACTTTCA+TGG - chr7.4:34768380-34768399 MS.gene95614:CDS 30.0%
AGTTATCTCTAACACTATGA+AGG - chr7.4:34768431-34768450 MS.gene95614:CDS 30.0%
TATAAAGGGATGTGAAACAA+TGG - chr7.4:34769013-34769032 MS.gene95614:intron 30.0%
TCTAACACTATGAAGGAAAA+GGG - chr7.4:34768438-34768457 MS.gene95614:CDS 30.0%
TTTCTCTCTCATGATTCAAA+GGG - chr7.4:34768837-34768856 MS.gene95614:intron 30.0%
TTTGAATCATGAGAGAGAAA+AGG + chr7.4:34768837-34768856 None:intergenic 30.0%
! TAAGAAACTCCTTTGTTTCT+TGG + chr7.4:34768350-34768369 None:intergenic 30.0%
! TTTTCTCTCTCATGATTCAA+AGG - chr7.4:34768836-34768855 MS.gene95614:intron 30.0%
!! AAGTTTGATCTAGTTCTGAA+CGG + chr7.4:34768377-34768396 None:intergenic 30.0%
!!! TTCAAGCTATTTTTAGTGCA+AGG - chr7.4:34769078-34769097 MS.gene95614:intron 30.0%
!!! TTGCCTTTTCAATTTGTTAG+GGG - chr7.4:34768807-34768826 MS.gene95614:intron 30.0%
AAACCTTGTTACTCGCTATA+AGG - chr7.4:34768500-34768519 MS.gene95614:CDS 35.0%
AAACTTTCATGGAGACAAAG+AGG - chr7.4:34768391-34768410 MS.gene95614:CDS 35.0%
AAAGAGGAACAAACAGCTTT+GGG - chr7.4:34768407-34768426 MS.gene95614:CDS 35.0%
AAATGCAAATGGAAGCTCAA+TGG - chr7.4:34769324-34769343 MS.gene95614:CDS 35.0%
AAGGATGCAAAGAATGCTAT+GGG - chr7.4:34769097-34769116 MS.gene95614:intron 35.0%
AAGGATTAGAGAAGAAGCTA+GGG - chr7.4:34769475-34769494 MS.gene95614:CDS 35.0%
AGATTCTTGAGGAGTTTATG+AGG - chr7.4:34769297-34769316 MS.gene95614:CDS 35.0%
ATACCTTATAGCGAGTAACA+AGG + chr7.4:34768506-34768525 None:intergenic 35.0%
CAACATGCTCATAACATCAT+AGG - chr7.4:34768273-34768292 MS.gene95614:CDS 35.0%
CAATTTGAAGGAGATTCTTG+AGG - chr7.4:34769286-34769305 MS.gene95614:CDS 35.0%
CTCTAACACTATGAAGGAAA+AGG - chr7.4:34768437-34768456 MS.gene95614:CDS 35.0%
GCTTGAAGCTCATTGTAAAA+TGG + chr7.4:34769065-34769084 None:intergenic 35.0%
TACCCCCTAACAAATTGAAA+AGG + chr7.4:34768813-34768832 None:intergenic 35.0%
TAGTGGTAGCAACAATTTGA+AGG - chr7.4:34769274-34769293 MS.gene95614:CDS 35.0%
TAGTGTTAACGTTGATGTCA+CGG - chr7.4:34768302-34768321 MS.gene95614:CDS 35.0%
TTAGGCAATGAAGAAAGTGA+GGG - chr7.4:34769182-34769201 MS.gene95614:intron 35.0%
! ATGGAGTATCCAAGAAACAA+AGG - chr7.4:34768338-34768357 MS.gene95614:CDS 35.0%
! GGTTAATGATGGAACAAAGA+TGG - chr7.4:34769432-34769451 MS.gene95614:CDS 35.0%
!! GTTGATGTTGTTGATGATGT+TGG + chr7.4:34768200-34768219 None:intergenic 35.0%
!!! TGCCTTTTCAATTTGTTAGG+GGG - chr7.4:34768808-34768827 MS.gene95614:intron 35.0%
AAGGAGGGTCAAAGAAGAAA+GGG - chr7.4:34769129-34769148 MS.gene95614:intron 40.0%
CAAAGAGGAACAAACAGCTT+TGG - chr7.4:34768406-34768425 MS.gene95614:CDS 40.0%
CAAGGATGCAAAGAATGCTA+TGG - chr7.4:34769096-34769115 MS.gene95614:intron 40.0%
GAAGGATTAGAGAAGAAGCT+AGG - chr7.4:34769474-34769493 MS.gene95614:CDS 40.0%
GAGAAAGGGAAGAACAAAGA+AGG - chr7.4:34769456-34769475 MS.gene95614:CDS 40.0%
GGAACAAAGATGGAGAGAAA+GGG - chr7.4:34769442-34769461 MS.gene95614:CDS 40.0%
GGAGAATGAGAGGTTAATGA+TGG - chr7.4:34769421-34769440 MS.gene95614:CDS 40.0%
GGTAAGATGATAATTGGTGG+AGG - chr7.4:34769242-34769261 MS.gene95614:CDS 40.0%
GGTGGAGGAAATGATAATAG+TGG - chr7.4:34769257-34769276 MS.gene95614:CDS 40.0%
GTTAGGCAATGAAGAAAGTG+AGG - chr7.4:34769181-34769200 MS.gene95614:intron 40.0%
GTTTCTTGGATACTCCATTG+AGG + chr7.4:34768336-34768355 None:intergenic 40.0%
TATGAGGCAGCAAATGCAAA+TGG - chr7.4:34769313-34769332 MS.gene95614:CDS 40.0%
TCACGGATAGATTTCCTCAA+TGG - chr7.4:34768319-34768338 MS.gene95614:CDS 40.0%
TCTGAGGATGAAGAAGAGTT+AGG - chr7.4:34769164-34769183 MS.gene95614:intron 40.0%
TGGAACAAAGATGGAGAGAA+AGG - chr7.4:34769441-34769460 MS.gene95614:CDS 40.0%
! GAGACAAAGAATGGAAGCAT+TGG - chr7.4:34769400-34769419 MS.gene95614:CDS 40.0%
!! TCAATGGATGGAAGCATTTG+AGG - chr7.4:34769340-34769359 MS.gene95614:CDS 40.0%
AGAAAGTGAGGGAGATCACA+AGG - chr7.4:34769193-34769212 MS.gene95614:intron 45.0%
AGGAGGGTCAAAGAAGAAAG+GGG - chr7.4:34769130-34769149 MS.gene95614:intron 45.0%
GAAAGTGAGGGAGATCACAA+GGG - chr7.4:34769194-34769213 MS.gene95614:intron 45.0%
GAAGGAGGGTCAAAGAAGAA+AGG - chr7.4:34769128-34769147 MS.gene95614:intron 45.0%
GATGGAGTGGAGACAAAGAA+TGG - chr7.4:34769391-34769410 MS.gene95614:CDS 45.0%
GCAAATGGAAGCTCAATGGA+TGG - chr7.4:34769328-34769347 MS.gene95614:CDS 45.0%
GGAGAATGAGAGGAGATTGA+AGG - chr7.4:34769367-34769386 MS.gene95614:CDS 45.0%
TGAGAGGAGATTGAAGGAGA+TGG - chr7.4:34769373-34769392 MS.gene95614:CDS 45.0%
!! GATGGAAGCATTTGAGGCAA+GGG - chr7.4:34769346-34769365 MS.gene95614:CDS 45.0%
!! TGGAAGCATTGGAGAATGAG+AGG - chr7.4:34769411-34769430 MS.gene95614:CDS 45.0%
CAGAACAGTGCAAGTGCAAG+TGG - chr7.4:34768475-34768494 MS.gene95614:CDS 50.0%
GGAGATTGAAGGAGATGGAG+TGG - chr7.4:34769378-34769397 MS.gene95614:CDS 50.0%
TTGAGGCAAGGGAGAATGAG+AGG - chr7.4:34769357-34769376 MS.gene95614:CDS 50.0%
! AGGGGTGCATGTATCTTCTG+AGG - chr7.4:34769148-34769167 MS.gene95614:intron 50.0%
! ATGCTATGGGCTGAAGCAGA+AGG - chr7.4:34769110-34769129 MS.gene95614:intron 50.0%
! TATGGGCTGAAGCAGAAGGA+GGG - chr7.4:34769114-34769133 MS.gene95614:intron 50.0%
!! GGATGGAAGCATTTGAGGCA+AGG - chr7.4:34769345-34769364 MS.gene95614:CDS 50.0%
! CTATGGGCTGAAGCAGAAGG+AGG - chr7.4:34769113-34769132 MS.gene95614:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 34768180 34769559 34768180 ID=MS.gene95614
chr7.4 mRNA 34768180 34769559 34768180 ID=MS.gene95614.t1;Parent=MS.gene95614
chr7.4 exon 34769218 34769559 34769218 ID=MS.gene95614.t1.exon1;Parent=MS.gene95614.t1
chr7.4 CDS 34769218 34769559 34769218 ID=cds.MS.gene95614.t1;Parent=MS.gene95614.t1
chr7.4 exon 34768180 34768719 34768180 ID=MS.gene95614.t1.exon2;Parent=MS.gene95614.t1
chr7.4 CDS 34768180 34768719 34768180 ID=cds.MS.gene95614.t1;Parent=MS.gene95614.t1
Gene Sequence

>MS.gene95614

ATGGAGGGTCATCATCTCCAACATCATCAACAACATCAACAACAACATCATCAACAACATCAACAGCAACATCAACAAAGACAACATCAACAACAACATGCTCATAACATCATAGGTACTAGTAGTGTTAACGTTGATGTCACGGATAGATTTCCTCAATGGAGTATCCAAGAAACAAAGGAGTTTCTTATGATCCGTTCAGAACTAGATCAAACTTTCATGGAGACAAAGAGGAACAAACAGCTTTGGGAAGTTATCTCTAACACTATGAAGGAAAAGGGTTATCATAGAAGTGCAGAACAGTGCAAGTGCAAGTGGAAAAACCTTGTTACTCGCTATAAGGTATTTCTATTCATATATATACATATATAAAAGAAATTAAAAAATTACACTATCAACCAATCATAAATCATTCTATGTGCATAATAATAAAATCTTTCATATAATATATTACTAACCAATCATAAATCATTCTTGTTTCAATAAATTTGCTTATAAAAAAATCTAATTAAAATTATTGCAATTAAGCAAGTCATGTATTTTGTTAAACTTTTTTTTTGGACAGATTTTTGTTAATCTTATAAACATACATAGATATATATACATACATATGCCACTTTTTATTTATTGCCTTTTCAATTTGTTAGGGGGTAACCTTTTCTCTCTCATGATTCAAAGGGTACTTTTAAAAAAAAAATAAAAGTTCATATTTAGTTCTATATATTAATATGTAACACTTTAACAAAATATATGATTTTTATATTGTGAGCTTTTCATGGCTGTTTGTTATATATATGTATAGTAAGACTATTGTTTTGTTTATGTTTTTGGGATATAAAGGGATGTGAAACAATGGAGCTAGAAGCCATGAGACAACAATTTCCATTTTACAATGAGCTTCAAGCTATTTTTAGTGCAAGGATGCAAAGAATGCTATGGGCTGAAGCAGAAGGAGGGTCAAAGAAGAAAGGGGTGCATGTATCTTCTGAGGATGAAGAAGAGTTAGGCAATGAAGAAAGTGAGGGAGATCACAAGGGCAACATTAAAAAGAAGAAGAAAAAAGGTAAGATGATAATTGGTGGAGGAAATGATAATAGTGGTAGCAACAATTTGAAGGAGATTCTTGAGGAGTTTATGAGGCAGCAAATGCAAATGGAAGCTCAATGGATGGAAGCATTTGAGGCAAGGGAGAATGAGAGGAGATTGAAGGAGATGGAGTGGAGACAAAGAATGGAAGCATTGGAGAATGAGAGGTTAATGATGGAACAAAGATGGAGAGAAAGGGAAGAACAAAGAAGGATTAGAGAAGAAGCTAGGGCTGAAAAGAGAGATGAATTAATCACTGCTTTGCTTGATAAGCTTACAAGAGAAGACATGTAG

Protein sequence

>MS.gene95614.t1

MEGHHLQHHQQHQQQHHQQHQQQHQQRQHQQQHAHNIIGTSSVNVDVTDRFPQWSIQETKEFLMIRSELDQTFMETKRNKQLWEVISNTMKEKGYHRSAEQCKCKWKNLVTRYKGCETMELEAMRQQFPFYNELQAIFSARMQRMLWAEAEGGSKKKGVHVSSEDEEELGNEESEGDHKGNIKKKKKKGKMIIGGGNDNSGSNNLKEILEEFMRQQMQMEAQWMEAFEARENERRLKEMEWRQRMEALENERLMMEQRWREREEQRRIREEARAEKRDELITALLDKLTREDM