AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99535


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99535.t1 MTR_5g042420 94.937 79 2 1 26 104 136 212 1.01e-50 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99535.t1 AT5G43750 72.165 97 24 2 8 104 119 212 8.42e-46 147

Find 18 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGATTTCAATGACAAATA+TGG 0.271183 1.3:-23483583 MS.gene99535:CDS
CTTTCCATGTTGTATATTTG+AGG 0.297169 1.3:+23483652 None:intergenic
ACTATAATTCCATGAGCATA+TGG 0.355011 1.3:-23483603 MS.gene99535:CDS
TTTGAGTATGGAATATTTGA+TGG 0.360719 1.3:-23484766 MS.gene99535:CDS
TTATAGGAAGAATTTGAGTA+TGG 0.374124 1.3:-23484778 MS.gene99535:intron
ATGACAAATATGGAGACCTT+TGG 0.379641 1.3:-23483573 MS.gene99535:CDS
TCAAAGTGATGAGGTTGATC+AGG 0.402133 1.3:+23483676 None:intergenic
TGACAAATATGGAGACCTTT+GGG 0.407790 1.3:-23483572 MS.gene99535:CDS
GAGTATTTGTTCATTGGAGC+AGG 0.434030 1.3:-23483739 MS.gene99535:CDS
GGTCATCATACTTTCAATGA+AGG 0.504834 1.3:-23484745 MS.gene99535:CDS
CAAATATACAACATGGAAAG+AGG 0.518390 1.3:-23483649 MS.gene99535:CDS
CTATAATTCCATGAGCATAT+GGG 0.524970 1.3:-23483602 MS.gene99535:CDS
ATTGAAATCCCATATGCTCA+TGG 0.535585 1.3:+23483594 None:intergenic
AATGAAGGTGAAGAAAAGAA+AGG 0.563141 1.3:-23484730 MS.gene99535:intron
ATTTGAGGTTCAAAGTGATG+AGG 0.590006 1.3:+23483667 None:intergenic
AGTATTTGTTCATTGGAGCA+GGG 0.597436 1.3:-23483738 MS.gene99535:CDS
TCTTGATAGTAAAATCCCAA+AGG 0.630483 1.3:+23483557 None:intergenic
TGAACCTCAAATATACAACA+TGG 0.679208 1.3:-23483656 MS.gene99535:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAATTAGAGAATA+AGG - chr1.3:23483824-23483843 MS.gene99535:intron 10.0%
!!! TTTTTTTTTTTATAGTACTA+TGG - chr1.3:23483564-23483583 MS.gene99535:CDS 10.0%
!! GTATATATATGAAAAAATAC+AGG + chr1.3:23483542-23483561 None:intergenic 15.0%
!! TATATATGAAAAAATACAGG+TGG + chr1.3:23483539-23483558 None:intergenic 20.0%
!!! CAATCACTTTTGTTTTTTAA+TGG - chr1.3:23483751-23483770 MS.gene99535:intron 20.0%
!!! CAATTAACATTTAGAATTAC+CGG + chr1.3:23484552-23484571 None:intergenic 20.0%
!!! TTTTACTATCAAGAAGAATA+AGG - chr1.3:23484857-23484876 MS.gene99535:intron 20.0%
! AACGATTATAGTCAAAATTG+TGG + chr1.3:23484442-23484461 None:intergenic 25.0%
! ACTAATGAATAAATAAGTCG+TGG - chr1.3:23484232-23484251 MS.gene99535:intron 25.0%
! AGAAATCAATTGATACATTG+AGG - chr1.3:23484597-23484616 MS.gene99535:intron 25.0%
! TAAGTACAATGTATGTTTCA+GGG - chr1.3:23484642-23484661 MS.gene99535:intron 25.0%
! TTATAGGAAGAATTTGAGTA+TGG - chr1.3:23483627-23483646 MS.gene99535:CDS 25.0%
! TTGTAATACACGTTAGAAAA+TGG + chr1.3:23484128-23484147 None:intergenic 25.0%
! TTTGAGTATGGAATATTTGA+TGG - chr1.3:23483639-23483658 MS.gene99535:CDS 25.0%
!! ATCAATTTCAGGAACATTTT+GGG - chr1.3:23484035-23484054 MS.gene99535:intron 25.0%
!! TGTATTTTGCATTATTGAGA+TGG - chr1.3:23484701-23484720 MS.gene99535:intron 25.0%
!!! AATGTTGATTTTATTGTCGA+AGG - chr1.3:23483874-23483893 MS.gene99535:intron 25.0%
!!! CTAATTTTGATTCTAATGCA+GGG - chr1.3:23484472-23484491 MS.gene99535:intron 25.0%
!!! TCTAATTTTGATTCTAATGC+AGG - chr1.3:23484471-23484490 MS.gene99535:intron 25.0%
!!! TTGTTTTGTGTGGATTTTAT+AGG - chr1.3:23483611-23483630 MS.gene99535:CDS 25.0%
AAGTACAATGTATGTTTCAG+GGG - chr1.3:23484643-23484662 MS.gene99535:intron 30.0%
AATGAAGGTGAAGAAAAGAA+AGG - chr1.3:23483675-23483694 MS.gene99535:CDS 30.0%
ACTATAATTCCATGAGCATA+TGG - chr1.3:23484802-23484821 MS.gene99535:intron 30.0%
ATAGTCAAAATTGTGGTCAA+GGG + chr1.3:23484435-23484454 None:intergenic 30.0%
CAAAGTTCATGATCAATTTC+AGG - chr1.3:23484024-23484043 MS.gene99535:intron 30.0%
CAAATATACAACATGGAAAG+AGG - chr1.3:23484756-23484775 MS.gene99535:CDS 30.0%
CTAAGTACAATGTATGTTTC+AGG - chr1.3:23484641-23484660 MS.gene99535:intron 30.0%
CTATAATTCCATGAGCATAT+GGG - chr1.3:23484803-23484822 MS.gene99535:intron 30.0%
CTGAAACATACATTGTACTT+AGG + chr1.3:23484643-23484662 None:intergenic 30.0%
CTTTCCATGTTGTATATTTG+AGG + chr1.3:23484756-23484775 None:intergenic 30.0%
TAGAAAAACATATACACGCT+TGG + chr1.3:23484003-23484022 None:intergenic 30.0%
TATAGTCAAAATTGTGGTCA+AGG + chr1.3:23484436-23484455 None:intergenic 30.0%
TGAACCTCAAATATACAACA+TGG - chr1.3:23484749-23484768 MS.gene99535:CDS 30.0%
TGGGATTTCAATGACAAATA+TGG - chr1.3:23484822-23484841 MS.gene99535:intron 30.0%
TTAATGGAAATTGAAGCATG+TGG - chr1.3:23483767-23483786 MS.gene99535:intron 30.0%
! GATCAATTTCAGGAACATTT+TGG - chr1.3:23484034-23484053 MS.gene99535:intron 30.0%
! TCAATTTCAGGAACATTTTG+GGG - chr1.3:23484036-23484055 MS.gene99535:intron 30.0%
! TCTTGATAGTAAAATCCCAA+AGG + chr1.3:23484851-23484870 None:intergenic 30.0%
!!! TGAGATTTGTTTGTTTTGTG+TGG - chr1.3:23483601-23483620 MS.gene99535:CDS 30.0%
AAACATACCTTGAAATCCAG+TGG + chr1.3:23484097-23484116 None:intergenic 35.0%
AACATACCTTGAAATCCAGT+GGG + chr1.3:23484096-23484115 None:intergenic 35.0%
AAGGATGACATAATAGCAGT+AGG - chr1.3:23484066-23484085 MS.gene99535:intron 35.0%
ACACGTTAGAAAATGGTACA+AGG + chr1.3:23484121-23484140 None:intergenic 35.0%
AGTACAATGTATGTTTCAGG+GGG - chr1.3:23484644-23484663 MS.gene99535:intron 35.0%
AGTATTTGTTCATTGGAGCA+GGG - chr1.3:23484667-23484686 MS.gene99535:intron 35.0%
ATGACAAATATGGAGACCTT+TGG - chr1.3:23484832-23484851 MS.gene99535:intron 35.0%
ATTGAAATCCCATATGCTCA+TGG + chr1.3:23484814-23484833 None:intergenic 35.0%
CCAATGGTAAGAGTTTGATA+TGG + chr1.3:23483924-23483943 None:intergenic 35.0%
CCATATCAAACTCTTACCAT+TGG - chr1.3:23483921-23483940 MS.gene99535:intron 35.0%
CTAATTGAACCACAATTTGG+AGG + chr1.3:23484191-23484210 None:intergenic 35.0%
GAGCTAATTGAACCACAATT+TGG + chr1.3:23484194-23484213 None:intergenic 35.0%
GGTCATCATACTTTCAATGA+AGG - chr1.3:23483660-23483679 MS.gene99535:CDS 35.0%
TAGTCAAAATTGTGGTCAAG+GGG + chr1.3:23484434-23484453 None:intergenic 35.0%
TGACAAATATGGAGACCTTT+GGG - chr1.3:23484833-23484852 MS.gene99535:intron 35.0%
! ACCACAATTTGGAGGTTTTA+CGG + chr1.3:23484183-23484202 None:intergenic 35.0%
! ATTTGAGGTTCAAAGTGATG+AGG + chr1.3:23484741-23484760 None:intergenic 35.0%
!!! AACATTTTGGGGTGCAATTA+AGG - chr1.3:23484047-23484066 MS.gene99535:intron 35.0%
ACATACCTTGAAATCCAGTG+GGG + chr1.3:23484095-23484114 None:intergenic 40.0%
ATTGAAGCATGTGGTACTCA+TGG - chr1.3:23483776-23483795 MS.gene99535:intron 40.0%
CTAATGCAGGGCTTATTTCA+TGG - chr1.3:23484484-23484503 MS.gene99535:intron 40.0%
GAGTATTTGTTCATTGGAGC+AGG - chr1.3:23484666-23484685 MS.gene99535:intron 40.0%
TCACAGCTCATTAGCTATTG+TGG - chr1.3:23483946-23483965 MS.gene99535:intron 40.0%
TTTGATACTGCAGAGGTTTG+TGG + chr1.3:23484393-23484412 None:intergenic 40.0%
! CCACAATTTGGAGGTTTTAC+GGG + chr1.3:23484182-23484201 None:intergenic 40.0%
! TCAAAGTGATGAGGTTGATC+AGG + chr1.3:23484732-23484751 None:intergenic 40.0%
ATACCTTGAAATCCAGTGGG+GGG + chr1.3:23484093-23484112 None:intergenic 45.0%
CAGCTCATTAGCTATTGTGG+AGG - chr1.3:23483949-23483968 MS.gene99535:intron 45.0%
CATACCTTGAAATCCAGTGG+GGG + chr1.3:23484094-23484113 None:intergenic 45.0%
CCCGTAAAACCTCCAAATTG+TGG - chr1.3:23484179-23484198 MS.gene99535:intron 45.0%
CTAATGAGCTGTGACACCAA+TGG + chr1.3:23483940-23483959 None:intergenic 45.0%
! AGTCGATTCTGCCTCAATTG+CGG + chr1.3:23484362-23484381 None:intergenic 45.0%
! GCAAGCTTTTGATACTGCAG+AGG + chr1.3:23484400-23484419 None:intergenic 45.0%
! TGGGGTATTCTTCGATGTTC+CGG - chr1.3:23484530-23484549 MS.gene99535:intron 45.0%
!! CAGGGGGAGTATTTGTTCAT+TGG - chr1.3:23484660-23484679 MS.gene99535:intron 45.0%
GAAGAATACCCCAGCAGCAA+TGG + chr1.3:23484523-23484542 None:intergenic 50.0%
TCGTGGTTGTGTTGCAACTG+AGG - chr1.3:23484249-23484268 MS.gene99535:intron 50.0%
AATACCCCAGCAGCAATGGC+TGG + chr1.3:23484519-23484538 None:intergenic 55.0%
ATAGCAGTAGGACCCCCCAC+TGG - chr1.3:23484078-23484097 MS.gene99535:intron 60.0%
CTGCACTGCAGCCGCAATTG+AGG - chr1.3:23484348-23484367 MS.gene99535:intron 60.0%
GCTGCAGTGCAGTGCAGTTG+TGG + chr1.3:23484340-23484359 None:intergenic 60.0%
GGACCCCCCACTGGATTTCA+AGG - chr1.3:23484087-23484106 MS.gene99535:intron 60.0%
TCCCACCAGCCATTGCTGCT+GGG - chr1.3:23484511-23484530 MS.gene99535:intron 60.0%
TGGTCAAGGGGCAGCTCAAG+TGG + chr1.3:23484422-23484441 None:intergenic 60.0%
TTCCCACCAGCCATTGCTGC+TGG - chr1.3:23484510-23484529 MS.gene99535:intron 60.0%
ACCCCAGCAGCAATGGCTGG+TGG + chr1.3:23484516-23484535 None:intergenic 65.0%
CCCACCAGCCATTGCTGCTG+GGG - chr1.3:23484512-23484531 MS.gene99535:intron 65.0%
CCCCAGCAGCAATGGCTGGT+GGG + chr1.3:23484515-23484534 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 23483528 23484899 23483528 ID=MS.gene99535
chr1.3 mRNA 23483528 23484899 23483528 ID=MS.gene99535.t1;Parent=MS.gene99535
chr1.3 exon 23484886 23484899 23484886 ID=MS.gene99535.t1.exon1;Parent=MS.gene99535.t1
chr1.3 CDS 23484886 23484899 23484886 ID=cds.MS.gene99535.t1;Parent=MS.gene99535.t1
chr1.3 exon 23484731 23484794 23484731 ID=MS.gene99535.t1.exon2;Parent=MS.gene99535.t1
chr1.3 CDS 23484731 23484794 23484731 ID=cds.MS.gene99535.t1;Parent=MS.gene99535.t1
chr1.3 exon 23483528 23483764 23483528 ID=MS.gene99535.t1.exon3;Parent=MS.gene99535.t1
chr1.3 CDS 23483528 23483764 23483528 ID=cds.MS.gene99535.t1;Parent=MS.gene99535.t1
Gene Sequence

>MS.gene99535

ATGGCATTCCACCTGTATTTTTTCATATATATACACTTTTTTTTTTTATAGTACTATGGTTTTGTTTTTGTGATGAGATTTGTTTGTTTTGTGTGGATTTTATAGGAAGAATTTGAGTATGGAATATTTGATGGTCATCATACTTTCAATGAAGGTGAAGAAAAGAAAGGTATTTGTTTTTTCATTGCTTACTTTCTTAGTTTTATAGTTTCTTTTCATTTTTCAATCACTTTTGTTTTTTAATGGAAATTGAAGCATGTGGTACTCATGGTAATTGTATACTTGTTATTTAAAAAAAAAAAAAAATTAGAGAATAAGGATATGTATAATTATTAATTAAAGAGACAATGTTGATTTTATTGTCGAAGGATTTGAACTCGATGTCTTATGTTACCATATCAAACTCTTACCATTGGTGTCACAGCTCATTAGCTATTGTGGAGGAAATCTATGATGACAAAAAATCTCATAACCAAGCGTGTATATGTTTTTCTAACAAAGTTCATGATCAATTTCAGGAACATTTTGGGGTGCAATTAAGGATGACATAATAGCAGTAGGACCCCCCACTGGATTTCAAGGTATGTTTTCCTTGTACCATTTTCTAACGTGTATTACAAGAATGTTTTAAAAACTCGACGAGTGATTGAGCCCGTAAAACCTCCAAATTGTGGTTCAATTAGCTCAAACAGTTGATCAGAATGACTAATGAATAAATAAGTCGTGGTTGTGTTGCAACTGAGGAGTTTTATAAACAGAAATGTTAGCGACTTGATCACAACATCAGCGATATCAAAGTATTTTTGTCTCCACAACTGCACTGCACTGCAGCCGCAATTGAGGCAGAATCGACTACATTTGACCACAAACCTCTGCAGTATCAAAAGCTTGCCACTTGAGCTGCCCCTTGACCACAATTTTGACTATAATCGTTGATTTCGATTCTAATTTTGATTCTAATGCAGGGCTTATTTCATGGCTCTTCCCACCAGCCATTGCTGCTGGGGTATTCTTCGATGTTCCGGTAATTCTAAATGTTAATTGACATCAAAGAAGCAATACTCAATCTAGAAATCAATTGATACATTGAGGAAGAAGTAATTGATCTGAATCCTAAGTACAATGTATGTTTCAGGGGGAGTATTTGTTCATTGGAGCAGGGATATTTACAATTGTATTTTGCATTATTGAGATGGATAAACCTGATCAACCTCATCACTTTGAACCTCAAATATACAACATGGAAAGAGGAGCTAGAGACAAGTTGATAAATGACTATAATTCCATGAGCATATGGGATTTCAATGACAAATATGGAGACCTTTGGGATTTTACTATCAAGAAGAATAAGGATGACATAATGAAGAGATAA

Protein sequence

>MS.gene99535.t1

MAFHLKNLSMEYLMVIILSMKVKKRKGEYLFIGAGIFTIVFCIIEMDKPDQPHHFEPQIYNMERGARDKLINDYNSMSIWDFNDKYGDLWDFTIKKNKDDIMKR