AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1171140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171140 MtrunA17_Chr8g0342451 98.588 425 6 0 1 425 1 425 0.0 868
Msa1171140 MtrunA17_Chr2g0330161 58.295 434 169 4 1 425 1 431 3.54e-176 499
Msa1171140 MtrunA17_Chr8g0366411 31.879 298 160 9 32 325 76 334 2.57e-28 116
Msa1171140 MtrunA17_Chr4g0049031 28.782 271 154 8 33 301 26 259 7.48e-27 112
Msa1171140 MtrunA17_Chr3g0085901 29.259 270 140 8 35 301 11 232 3.22e-26 108
Msa1171140 MtrunA17_Chr7g0221741 36.548 197 101 7 27 216 8 187 1.48e-24 103
Msa1171140 MtrunA17_Chr5g0440681 32.787 183 113 2 34 216 24 196 2.98e-24 103
Msa1171140 MtrunA17_Chr7g0252201 27.143 280 162 8 27 301 8 250 6.93e-24 100
Msa1171140 MtrunA17_Chr4g0023631 29.412 306 179 8 24 327 4 274 5.24e-23 100
Msa1171140 MtrunA17_Chr3g0085941 27.574 272 152 8 34 301 13 243 1.37e-22 98.2
Msa1171140 MtrunA17_Chr3g0104731 29.964 277 145 10 34 301 18 254 3.06e-22 95.9
Msa1171140 MtrunA17_Chr5g0424701 25.455 275 162 7 35 306 16 250 8.58e-22 94.4
Msa1171140 MtrunA17_Chr5g0427711 28.881 277 151 10 35 304 3 240 2.07e-21 94.7
Msa1171140 MtrunA17_Chr1g0199541 29.779 272 148 10 34 300 24 257 7.07e-21 92.0
Msa1171140 MtrunA17_Chr3g0099441 28.731 268 157 6 34 280 26 280 9.65e-21 94.4
Msa1171140 MtrunA17_Chr6g0486911 29.242 277 151 10 35 306 21 257 1.03e-20 93.6
Msa1171140 MtrunA17_Chr3g0084241 28.808 302 164 14 35 327 7 266 1.13e-20 93.2
Msa1171140 MtrunA17_Chr3g0091201 34.409 186 107 5 38 216 32 209 2.24e-20 91.7
Msa1171140 MtrunA17_Chr7g0249591 27.881 269 155 7 35 302 14 244 2.25e-20 92.8
Msa1171140 MtrunA17_Chr3g0099461 27.888 251 149 4 34 260 26 268 3.13e-20 92.4
Msa1171140 MtrunA17_Chr3g0084261 27.508 309 175 9 34 334 24 291 5.08e-20 91.3
Msa1171140 MtrunA17_Chr1g0181881 34.058 138 84 2 35 172 18 148 1.45e-19 85.5
Msa1171140 MtrunA17_Chr8g0362531 29.348 276 174 7 11 280 21 281 1.55e-19 90.1
Msa1171140 MtrunA17_Chr6g0460041 28.044 271 151 7 34 301 39 268 2.36e-19 88.2
Msa1171140 MtrunA17_Chr4g0049041 31.746 189 119 4 34 216 23 207 2.88e-19 89.4
Msa1171140 MtrunA17_Chr6g0457641 27.798 277 144 9 34 301 50 279 5.84e-19 87.8
Msa1171140 MtrunA17_Chr4g0011401 31.250 192 115 6 33 216 143 325 1.21e-18 88.2
Msa1171140 MtrunA17_Chr3g0085961 25.902 305 190 10 35 328 14 293 1.45e-18 87.0
Msa1171140 MtrunA17_Chr4g0011331 29.412 289 146 12 35 316 153 390 2.00e-18 87.0
Msa1171140 MtrunA17_Chr3g0082451 30.769 273 141 12 34 301 9 238 4.71e-18 85.5
Msa1171140 MtrunA17_Chr6g0488371 34.783 138 87 2 36 172 11 146 5.86e-18 82.4
Msa1171140 MtrunA17_Chr3g0136291 29.902 204 119 8 36 232 14 200 6.56e-18 84.3
Msa1171140 MtrunA17_Chr3g0104721 28.159 277 153 10 34 301 27 266 6.73e-18 84.0
Msa1171140 MtrunA17_Chr4g0049111 29.717 212 113 6 35 243 42 220 8.96e-18 85.5
Msa1171140 MtrunA17_Chr3g0082431 32.579 221 111 8 34 252 9 193 9.63e-18 84.3
Msa1171140 MtrunA17_Chr7g0220351 32.161 199 109 6 32 216 144 330 1.22e-17 85.1
Msa1171140 MtrunA17_Chr4g0023731 55.072 69 31 0 37 105 21 89 1.56e-17 80.9
Msa1171140 MtrunA17_Chr4g0049071 27.442 215 140 5 11 216 25 232 1.83e-17 84.0
Msa1171140 MtrunA17_Chr3g0078681 25.954 262 163 6 35 296 24 254 1.91e-17 83.2
Msa1171140 MtrunA17_Chr3g0132661 29.577 213 134 5 32 236 22 226 2.40e-17 83.6
Msa1171140 MtrunA17_Chr3g0091211 36.441 118 66 4 32 143 7 121 2.48e-17 81.3
Msa1171140 MtrunA17_Chr6g0488401 26.872 227 137 4 36 262 10 207 2.63e-17 83.2
Msa1171140 MtrunA17_Chr6g0486921 26.471 272 160 9 35 301 18 254 2.93e-17 83.6
Msa1171140 MtrunA17_Chr6g0452031 32.692 208 119 7 23 212 3 207 3.55e-17 82.0
Msa1171140 MtrunA17_Chr4g0049121 36.134 119 70 3 34 149 30 145 3.91e-17 83.6
Msa1171140 MtrunA17_Chr3g0139601 36.471 170 94 6 30 195 7 166 4.64e-17 83.2
Msa1171140 MtrunA17_Chr3g0078891 24.933 373 241 10 35 399 10 351 4.67e-17 82.8
Msa1171140 MtrunA17_Chr3g0077781 27.778 252 135 9 35 280 16 226 5.92e-17 82.0
Msa1171140 MtrunA17_Chr3g0087471 33.913 115 75 1 31 145 21 134 6.65e-17 77.0
Msa1171140 MtrunA17_Chr3g0091161 33.553 152 90 5 34 183 13 155 7.90e-17 81.6
Msa1171140 MtrunA17_Chr3g0078731 25.954 262 163 6 35 296 10 240 8.97e-17 81.6
Msa1171140 MtrunA17_Chr6g0457681 27.305 282 144 9 36 301 55 291 9.05e-17 82.0
Msa1171140 MtrunA17_Chr6g0460051 38.679 106 63 2 35 139 51 155 1.30e-16 77.0
Msa1171140 MtrunA17_Chr5g0424761 30.045 223 116 6 35 255 17 201 1.36e-16 80.9
Msa1171140 MtrunA17_Chr7g0221701 32.530 166 96 6 58 216 4 160 1.41e-16 80.1
Msa1171140 MtrunA17_Chr4g0005311 33.571 140 84 3 34 172 24 155 1.55e-16 81.3
Msa1171140 MtrunA17_Chr3g0084251 32.692 156 97 4 18 172 8 156 1.76e-16 81.6
Msa1171140 MtrunA17_Chr3g0078191 33.824 136 81 3 38 172 50 177 1.80e-16 80.9
Msa1171140 MtrunA17_Chr2g0325081 27.491 291 164 8 35 295 13 286 2.61e-16 80.1
Msa1171140 MtrunA17_Chr8g0364381 38.182 110 66 1 35 144 67 174 2.83e-16 80.5
Msa1171140 MtrunA17_Chr5g0419311 31.188 202 114 6 35 225 29 216 3.06e-16 79.3
Msa1171140 MtrunA17_Chr7g0259181 32.738 168 102 5 30 194 22 181 3.09e-16 80.5
Msa1171140 MtrunA17_Chr5g0419301 30.732 205 117 6 35 228 29 219 4.07e-16 80.1
Msa1171140 MtrunA17_Chr3g0086141 33.880 183 100 8 36 206 3 176 7.34e-16 76.3
Msa1171140 MtrunA17_Chr3g0100081 27.431 288 159 12 36 301 25 284 7.83e-16 78.6
Msa1171140 MtrunA17_Chr4g0049131 34.400 125 75 3 35 157 6 125 1.25e-15 73.6
Msa1171140 MtrunA17_Chr6g0460081 26.667 270 156 8 34 301 32 261 2.39e-15 77.4
Msa1171140 MtrunA17_Chr3g0077791 32.258 124 83 1 31 154 12 134 3.05e-15 76.3
Msa1171140 MtrunA17_Chr3g0085951 26.132 287 159 9 35 313 2 243 6.52e-15 76.3
Msa1171140 MtrunA17_Chr7g0236151 31.098 164 104 3 35 195 8 165 8.53e-15 75.1
Msa1171140 MtrunA17_Chr7g0236191 31.098 164 104 3 35 195 8 165 8.53e-15 75.1
Msa1171140 MtrunA17_Chr3g0086041 32.168 143 92 3 32 172 22 161 1.12e-14 75.5
Msa1171140 MtrunA17_Chr7g0236101 31.655 139 89 2 35 173 8 140 1.81e-14 71.2
Msa1171140 MtrunA17_Chr4g0058061 28.421 190 120 6 33 216 155 334 2.46e-14 74.7
Msa1171140 MtrunA17_Chr1g0188701 23.938 259 164 9 35 277 3 244 2.88e-14 72.8
Msa1171140 MtrunA17_Chr1g0166251 29.798 198 124 4 36 230 11 196 3.18e-14 73.9
Msa1171140 MtrunA17_Chr7g0246351 29.148 223 131 8 35 246 5 211 6.38e-14 73.2
Msa1171140 MtrunA17_Chr1g0164901 33.486 218 118 8 36 234 9 218 6.49e-14 72.8
Msa1171140 MtrunA17_Chr7g0227851 29.834 181 122 4 36 212 11 190 9.40e-14 72.4
Msa1171140 MtrunA17_Chr4g0048151 26.754 228 137 7 3 216 2 213 1.01e-13 72.8
Msa1171140 MtrunA17_Chr7g0236211 33.333 141 87 4 35 172 11 147 1.54e-13 71.6
Msa1171140 MtrunA17_Chr7g0273871 31.884 138 88 2 36 172 13 145 1.55e-13 71.6
Msa1171140 MtrunA17_Chr3g0137681 29.108 213 132 6 35 232 9 217 2.87e-13 70.9
Msa1171140 MtrunA17_Chr3g0093361 29.452 146 97 2 30 173 37 178 2.93e-13 70.9
Msa1171140 MtrunA17_Chr4g0027231 31.325 249 122 10 26 227 91 337 3.61e-13 71.2
Msa1171140 MtrunA17_Chr4g0027471 30.986 213 110 6 29 205 3 214 3.80e-13 70.9
Msa1171140 MtrunA17_Chr3g0091171 30.464 151 93 4 36 181 5 148 3.80e-13 70.5
Msa1171140 MtrunA17_Chr4g0011321 29.474 190 93 8 35 216 29 185 3.97e-13 69.7
Msa1171140 MtrunA17_Chr7g0236221 35.345 116 65 3 35 146 8 117 4.43e-13 66.2
Msa1171140 MtrunA17_Chr7g0227821 31.818 110 72 1 36 145 11 117 5.12e-13 68.6
Msa1171140 MtrunA17_Chr4g0049061 35.000 100 65 0 35 134 34 133 5.57e-13 66.6
Msa1171140 MtrunA17_Chr2g0319401 33.735 166 94 5 35 194 26 181 7.23e-13 69.3
Msa1171140 MtrunA17_Chr6g0457621 26.812 276 148 11 34 301 32 261 7.53e-13 69.7
Msa1171140 MtrunA17_Chr4g0027411 30.841 214 111 7 36 212 9 222 7.63e-13 70.1
Msa1171140 MtrunA17_Chr7g0227641 31.193 109 75 0 36 144 11 119 7.92e-13 69.7
Msa1171140 MtrunA17_Chr2g0331741 24.415 299 176 9 34 299 78 359 9.72e-13 69.7
Msa1171140 MtrunA17_Chr2g0319411 32.323 198 109 8 30 214 16 201 9.91e-13 69.3
Msa1171140 MtrunA17_Chr7g0226481 29.193 161 107 4 36 194 11 166 1.35e-12 68.6
Msa1171140 MtrunA17_Chr1g0154471 29.381 194 91 6 29 199 10 180 1.97e-12 68.6
Msa1171140 MtrunA17_Chr3g0138571 29.091 165 110 3 30 194 7 164 2.31e-12 68.2
Msa1171140 MtrunA17_Chr3g0124041 40.278 72 43 0 34 105 10 81 3.59e-12 62.8
Msa1171140 MtrunA17_Chr7g0262091 30.247 162 104 5 36 194 18 173 3.93e-12 67.4
Msa1171140 MtrunA17_Chr4g0076481 28.667 150 94 4 23 172 17 153 4.10e-12 65.9
Msa1171140 MtrunA17_Chr1g0150411 28.311 219 106 7 14 208 4 195 4.24e-12 67.8
Msa1171140 MtrunA17_Chr2g0325431 28.846 208 112 5 14 203 4 193 4.29e-12 67.8
Msa1171140 MtrunA17_Chr7g0274231 25.333 150 109 1 35 181 11 160 4.65e-12 66.2
Msa1171140 MtrunA17_Chr1g0150401 28.205 234 134 6 14 229 4 221 4.72e-12 67.4
Msa1171140 MtrunA17_Chr1g0148651 30.000 220 116 8 36 232 326 530 5.95e-12 67.8
Msa1171140 MtrunA17_Chr1g0148651 30.804 224 115 9 36 255 7 194 2.66e-11 65.5
Msa1171140 MtrunA17_Chr3g0138371 40.278 72 43 0 35 106 19 90 6.28e-12 67.0
Msa1171140 MtrunA17_Chr7g0227771 27.841 176 103 4 35 198 3 166 8.12e-12 64.3
Msa1171140 MtrunA17_Chr8g0385451 29.121 182 115 6 34 209 13 186 1.09e-11 64.7
Msa1171140 MtrunA17_Chr8g0361241 31.159 138 92 2 36 172 12 147 1.24e-11 65.1
Msa1171140 MtrunA17_Chr4g0027441 30.797 276 151 13 34 283 7 268 1.52e-11 65.9
Msa1171140 MtrunA17_Chr4g0041581 28.000 175 101 6 35 198 22 182 1.66e-11 65.9
Msa1171140 MtrunA17_Chr1g0212411 25.430 291 176 10 35 299 31 306 1.84e-11 65.1
Msa1171140 MtrunA17_Chr1g0148661 28.902 173 103 4 36 199 18 179 2.75e-11 63.9
Msa1171140 MtrunA17_Chr1g0212391 30.857 175 93 5 35 194 25 186 2.75e-11 64.3
Msa1171140 MtrunA17_Chr2g0278121 29.412 153 91 5 36 172 16 167 2.84e-11 65.1
Msa1171140 MtrunA17_Chr3g0142561 32.353 102 66 2 23 123 4 103 2.84e-11 60.8
Msa1171140 MtrunA17_Chr3g0084231 40.278 72 43 0 33 104 22 93 3.09e-11 64.7
Msa1171140 MtrunA17_Chr8g0361351 27.570 214 137 7 36 236 12 220 3.67e-11 64.3
Msa1171140 MtrunA17_Chr7g0227621 45.000 60 33 0 36 95 11 70 4.20e-11 59.7
Msa1171140 MtrunA17_Chr1g0148641 29.379 177 110 3 33 199 28 199 4.45e-11 64.3
Msa1171140 MtrunA17_Chr4g0023311 28.033 239 134 9 15 228 10 235 4.94e-11 64.7
Msa1171140 MtrunA17_Chr1g0197671 35.227 176 102 6 34 205 19 186 6.17e-11 62.0
Msa1171140 MtrunA17_Chr2g0303231 29.885 174 99 5 36 194 25 190 6.78e-11 63.9
Msa1171140 MtrunA17_Chr1g0170171 27.350 117 83 2 31 146 20 135 7.08e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171140 AT1G78840.1 26.721 247 161 7 41 286 11 238 3.08e-14 74.7
Msa1171140 AT1G78840.2 26.721 247 161 7 41 286 11 238 3.08e-14 74.7
Msa1171140 AT5G53840.1 34.641 153 84 6 33 180 15 156 4.14e-14 74.3
Msa1171140 AT1G51370.2 29.864 221 131 10 29 241 11 215 1.59e-13 72.0
Msa1171140 AT1G51370.3 29.412 221 132 9 29 241 11 215 1.80e-13 72.0
Msa1171140 AT1G51370.1 29.412 221 132 9 29 241 11 215 2.34e-13 72.0
Msa1171140 AT5G22720.1 30.612 147 95 3 29 172 16 158 2.47e-13 72.0
Msa1171140 AT1G69630.1 28.313 166 113 4 31 194 13 174 1.08e-12 70.1
Msa1171140 AT5G02920.1 34.247 146 82 5 35 178 27 160 1.16e-12 68.2
Msa1171140 AT5G02930.1 33.125 160 92 6 36 191 28 176 1.30e-12 69.7
Msa1171140 AT4G03220.1 29.070 258 138 12 36 278 24 251 3.67e-12 68.6
Msa1171140 AT5G56370.3 30.864 162 106 3 36 196 2 158 3.31e-11 64.7
Msa1171140 AT1G16930.2 29.762 168 102 5 34 195 13 170 4.43e-11 65.1
Msa1171140 AT1G16930.1 29.762 168 102 5 34 195 13 170 4.43e-11 65.1
Msa1171140 AT1G16930.3 29.762 168 102 5 34 195 13 170 4.43e-11 65.1
Msa1171140 AT5G53635.1 30.534 262 129 13 38 280 2 229 4.43e-11 64.7
Msa1171140 AT5G56370.1 30.864 162 106 3 36 196 2 158 4.91e-11 64.7
Msa1171140 AT5G56370.2 30.864 162 106 3 36 196 2 158 4.91e-11 64.7
Msa1171140 AT5G56420.1 28.333 180 90 5 33 194 3 161 7.42e-11 64.3
Msa1171140 AT5G56420.2 28.333 180 90 5 33 194 3 161 7.42e-11 64.3
Msa1171140 AT5G56420.3 28.333 180 90 5 33 194 3 161 7.42e-11 64.3
Msa1171140 AT4G13985.1 29.108 213 118 6 36 232 19 214 9.14e-11 63.9

Find 133 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTTCCTTGATGGGTTT+TGG 0.127648 8_1:-747875 Msa1171140:five_prime_UTR
TTTCTCTCATGATTCAATTT+TGG 0.194782 8_1:+743474 None:intergenic
TGAGTTTAGTCTTCCTTCTT+TGG 0.196025 8_1:-746087 Msa1171140:CDS
CGAAAAGGTGGAGGTATATT+TGG 0.219974 8_1:-746823 Msa1171140:CDS
TTCTTATTTACTATCGCTTT+GGG 0.227303 8_1:-746691 Msa1171140:CDS
TGAGATTGTGAATATTTAAA+AGG 0.239480 8_1:-745606 Msa1171140:intron
TAGCTTTGCCCTATGTTATA+CGG 0.250080 8_1:-746333 Msa1171140:CDS
AATGTTTGTCTTATAGTAAA+TGG 0.271082 8_1:-745730 Msa1171140:CDS
TCAATGGATAATTTCTAGTT+TGG 0.273372 8_1:-747111 Msa1171140:five_prime_UTR
GGGTACTGGATTTCCATTTC+GGG 0.290310 8_1:-743447 None:intergenic
TGGTGGACAGCCAGAGGTTT+TGG 0.297601 8_1:+746577 None:intergenic
AGGACTCAAATCTGACTTAA+AGG 0.311937 8_1:+746244 None:intergenic
AACATAGGGCAAAGCTATTT+AGG 0.315127 8_1:+746339 None:intergenic
GAAACCAACTCACATATTAA+TGG 0.316772 8_1:+747835 None:intergenic
AAGTAATGGGTTGTTGAAAA+AGG 0.320619 8_1:+748009 None:intergenic
GGGGTACTGGATTTCCATTT+CGG 0.322245 8_1:-743448 Msa1171140:three_prime_UTR
AAGACAAAATATGATGAATA+AGG 0.325111 8_1:+746885 None:intergenic
TTACTATAAGACAAACATTC+TGG 0.330922 8_1:+745734 None:intergenic
TTCTGTTTGTTTATGAGGAT+TGG 0.345937 8_1:-747789 Msa1171140:five_prime_UTR
AAAACTAGTTTACAGGAATC+TGG 0.348610 8_1:-747010 Msa1171140:CDS
ACAAACGTCTCTTCTCCTTT+TGG 0.350443 8_1:-746485 Msa1171140:CDS
GTTCTTATTTACTATCGCTT+TGG 0.358076 8_1:-746692 Msa1171140:CDS
TTTGAGGCTCCTTCAATGAT+TGG 0.364907 8_1:+747697 None:intergenic
TTTGGAGGGTAGTAGTTTGA+TGG 0.365819 8_1:-747093 Msa1171140:five_prime_UTR
GAGGTTTCTGTTTGTTTATG+AGG 0.369660 8_1:-747794 Msa1171140:five_prime_UTR
GAGAATTTGGTACCTGACTA+TGG 0.374270 8_1:+748034 None:intergenic
TTTGTTTATGAGGATTGGTT+TGG 0.379391 8_1:-747784 Msa1171140:five_prime_UTR
TCATGAATTACACGAACCTT+TGG 0.385218 8_1:-746045 Msa1171140:CDS
GTGAAGGTTAACCCAACATT+TGG 0.386881 8_1:-743714 Msa1171140:three_prime_UTR
ACTTCCGATAACACCCGAAA+TGG 0.387108 8_1:+743434 None:intergenic
GATACTTTGCACAGAAGGTT+TGG 0.387198 8_1:-743753 Msa1171140:three_prime_UTR
AATGCCGAAAACTAGTTTAC+AGG 0.387247 8_1:-747017 Msa1171140:CDS
TTTGTTGCAAATTTCCTTGA+TGG 0.388798 8_1:-747882 Msa1171140:five_prime_UTR
TGGTTTGATCAATATGTTAC+GGG 0.407463 8_1:-745985 Msa1171140:CDS
TTCAATTTCAGGTTACTTGT+GGG 0.410804 8_1:-745775 Msa1171140:intron
ACTATGGAAGATGATGATAA+TGG 0.413546 8_1:+748050 None:intergenic
AATCTCCGGGCCAAAACCTC+TGG 0.418965 8_1:-746587 Msa1171140:CDS
TTTCAAGAAGTTTGTGTTGT+GGG 0.420527 8_1:-746747 Msa1171140:CDS
TTTAGTCTTCCTTCTTTGGA+TGG 0.422418 8_1:-746083 Msa1171140:CDS
TTATCCATTGAATAGCTGAT+TGG 0.425310 8_1:+747123 None:intergenic
TTGTTGCAAATTTCCTTGAT+GGG 0.435731 8_1:-747881 Msa1171140:five_prime_UTR
AGTTCGGCTAAACTTGCAAA+TGG 0.436775 8_1:+746392 None:intergenic
ACTACAATTATTCACACATA+AGG 0.437324 8_1:+743620 None:intergenic
TTATGAGGATTGGTTTGGAT+TGG 0.439636 8_1:-747779 Msa1171140:five_prime_UTR
AGGATTGGTTTGGATTGGAT+TGG 0.440729 8_1:-747774 Msa1171140:five_prime_UTR
CATCATAAGGATCCAAATGT+TGG 0.448727 8_1:+743702 None:intergenic
ATTGCTTGGTATGGTTTGAA+TGG 0.450647 8_1:-743664 Msa1171140:three_prime_UTR
GGTTTGAATGGGAAATGATT+CGG 0.452133 8_1:-743652 Msa1171140:three_prime_UTR
ATATGATGAATAAGGCAATC+TGG 0.453620 8_1:+746893 None:intergenic
AGAGGCAACGGAATTTCAAT+TGG 0.454657 8_1:+746557 None:intergenic
TATGTTACGGGGACATCATA+AGG 0.455100 8_1:-745973 Msa1171140:CDS
TTCAGTGTGTGTTGTGAACC+TGG 0.457555 8_1:-747040 Msa1171140:five_prime_UTR
GAACTTGTGTTCTATCGAAA+AGG 0.461513 8_1:-746838 Msa1171140:CDS
GCATGTCACCTAGCCTGTCC+TGG 0.463290 8_1:+746915 None:intergenic
TGCGGAAGTAGGCAGTTATA+TGG 0.463855 8_1:+745651 None:intergenic
ATTCAATTTCAGGTTACTTG+TGG 0.467186 8_1:-745776 Msa1171140:intron
ATGGATAATTTCTAGTTTGG+AGG 0.468678 8_1:-747108 Msa1171140:five_prime_UTR
TTGGAGGGTAGTAGTTTGAT+GGG 0.469511 8_1:-747092 Msa1171140:five_prime_UTR
TCCAATGATTTGCAACCAAA+AGG 0.472560 8_1:+746470 None:intergenic
TATCATCATCTTCCATAGTC+AGG 0.473558 8_1:-748046 Msa1171140:five_prime_UTR
CTGATATAACCAATCATTGA+AGG 0.473751 8_1:-747706 Msa1171140:five_prime_UTR
TAAGTCAGATTTGAGTCCTA+AGG 0.473916 8_1:-746240 Msa1171140:CDS
ACAAGGATTATTGCTTGGTA+TGG 0.473997 8_1:-743673 Msa1171140:three_prime_UTR
GTGTGTTGTTAGATTGTACA+TGG 0.475996 8_1:-747164 Msa1171140:intron
ATGGTTTGATCAATATGTTA+CGG 0.476561 8_1:-745986 Msa1171140:CDS
AAAGGAGAAGAGACGTTTGT+AGG 0.478600 8_1:+746488 None:intergenic
AAATTGACACAATTAGCAAA+TGG 0.484411 8_1:+746293 None:intergenic
GCAGATATCACAAAATCCTT+AGG 0.486149 8_1:+746224 None:intergenic
TCCTTGTGTATATCATCATA+AGG 0.489284 8_1:+743689 None:intergenic
GCATAACATTGAATAATTTG+AGG 0.490252 8_1:+747681 None:intergenic
AAATGGAAGTCATTGAACTT+TGG 0.491699 8_1:-745713 Msa1171140:CDS
CTATGGAAGATGATGATAAT+GGG 0.493519 8_1:+748051 None:intergenic
TTATACGGGAATGGAGCGTC+TGG 0.494898 8_1:-746318 Msa1171140:CDS
GGCAACGGAATTTCAATTGG+TGG 0.499782 8_1:+746560 None:intergenic
TGAAGGATACTTTGCACAGA+AGG 0.501350 8_1:-743758 Msa1171140:three_prime_UTR
ATGATAACTTTACTATTCCA+TGG 0.502019 8_1:+747935 None:intergenic
TGCCCTATGTTATACGGGAA+TGG 0.502035 8_1:-746327 Msa1171140:CDS
ATCATAAGGATCCAAATGTT+GGG 0.503049 8_1:+743703 None:intergenic
AAAAGAGAGGAGACTTTACA+TGG 0.503943 8_1:-746005 Msa1171140:CDS
AGCTTTGCCCTATGTTATAC+GGG 0.507164 8_1:-746332 Msa1171140:CDS
TTGCTTGGTATGGTTTGAAT+GGG 0.507874 8_1:-743663 Msa1171140:three_prime_UTR
GAGAAGAGACGTTTGTAGGT+TGG 0.512178 8_1:+746492 None:intergenic
CAGCAAATCATTCACTCGGT+TGG 0.512294 8_1:-746774 Msa1171140:CDS
TATACACAAGGATTATTGCT+TGG 0.513544 8_1:-743678 Msa1171140:three_prime_UTR
AAGAAATCAGAATTAATCTC+CGG 0.513913 8_1:-746601 Msa1171140:CDS
TTGTGTTGCGACAGGACACC+AGG 0.527358 8_1:-746933 Msa1171140:CDS
ATTGAATATGCAGCATTACA+TGG 0.527512 8_1:-746629 Msa1171140:CDS
AAAAGATCCAAAACCCATCA+AGG 0.531804 8_1:+747868 None:intergenic
ATGATCAATCCATCCAAAGA+AGG 0.536851 8_1:+746074 None:intergenic
CTGAGAGCAATTGCGGAAGT+AGG 0.536899 8_1:+745640 None:intergenic
ACACCTTTGATCGGAAGTGC+CGG 0.540420 8_1:+747648 None:intergenic
TTGCGACAGGACACCAGGAC+AGG 0.540944 8_1:-746928 Msa1171140:CDS
CTCAAAGAAGGCACAAGGTG+TGG 0.541752 8_1:+746106 None:intergenic
GCTCCATTCCCGTATAACAT+AGG 0.543154 8_1:+746324 None:intergenic
CTCCATTCCCGTATAACATA+GGG 0.545398 8_1:+746325 None:intergenic
TTTGCTGTTGAAGTACAGAC+AGG 0.545777 8_1:+746790 None:intergenic
TGAACCAATCAGCTATTCAA+TGG 0.553532 8_1:-747127 Msa1171140:five_prime_UTR
TTCAATTGGTGGACAGCCAG+AGG 0.557133 8_1:+746571 None:intergenic
CTTATGCATTGCTGAGGTGA+AGG 0.559824 8_1:-743730 Msa1171140:three_prime_UTR
TGCAAATGGATTTGAAAAGT+CGG 0.561253 8_1:+746406 None:intergenic
TGGATAATTTCTAGTTTGGA+GGG 0.562228 8_1:-747107 Msa1171140:five_prime_UTR
AGGAAGACTAAACTCAAAGA+AGG 0.562750 8_1:+746094 None:intergenic
CTATACCGCAACTGTACAGA+AGG 0.567862 8_1:+745932 None:intergenic
TCAATTTCAGGTTACTTGTG+GGG 0.572484 8_1:-745774 Msa1171140:intron
CGGTCGGACACACCTTTGAT+CGG 0.575160 8_1:+747639 None:intergenic
CTACATGCTGAGAGCAATTG+CGG 0.575217 8_1:+745633 None:intergenic
GCACCGGCACTTCCGATCAA+AGG 0.579976 8_1:-747651 Msa1171140:intron
ATTTCAAGAAGTTTGTGTTG+TGG 0.581606 8_1:-746748 Msa1171140:CDS
AGACTCAAGTTACTGAGTTG+CGG 0.587346 8_1:+746200 None:intergenic
AGAAATCAGAATTAATCTCC+GGG 0.587391 8_1:-746600 Msa1171140:CDS
ACAGGACACCAGGACAGGCT+AGG 0.588272 8_1:-746923 Msa1171140:CDS
TTTGAAAAGTCGGCTGCGGT+AGG 0.591591 8_1:+746416 None:intergenic
ATGTTATGCATCAATAGCAC+CGG 0.594570 8_1:-747667 Msa1171140:five_prime_UTR
AAGTCATTGAACTTTGGAGT+GGG 0.596146 8_1:-745707 Msa1171140:CDS
CTTGTGTTCTATCGAAAAGG+TGG 0.601646 8_1:-746835 Msa1171140:CDS
GTGTTCTATCGAAAAGGTGG+AGG 0.603379 8_1:-746832 Msa1171140:CDS
GAAGTCATTGAACTTTGGAG+TGG 0.607082 8_1:-745708 Msa1171140:CDS
ACTATCCTTCTGTACAGTTG+CGG 0.608906 8_1:-745937 Msa1171140:intron
GTGTATAATTGTGTTGCGAC+AGG 0.610973 8_1:-746941 Msa1171140:CDS
CTACGATCTTACATCTAAAG+CGG 0.616468 8_1:+746168 None:intergenic
TGGATTTGAAAAGTCGGCTG+CGG 0.623356 8_1:+746412 None:intergenic
AATCTGGTTCTGTTATGAAG+AGG 0.633572 8_1:-746994 Msa1171140:CDS
CTAAACTCAAAGAAGGCACA+AGG 0.634438 8_1:+746101 None:intergenic
TCAACAGCAAATCATTCACT+CGG 0.634747 8_1:-746778 Msa1171140:CDS
TGTTGTTAGATTGTACATGG+TGG 0.636802 8_1:-747161 Msa1171140:intron
GTTTGGCTTATGCATTGCTG+AGG 0.638849 8_1:-743736 Msa1171140:three_prime_UTR
TCTTATTTACTATCGCTTTG+GGG 0.648748 8_1:-746690 Msa1171140:CDS
ATCTCATCGAAATAAAAGAG+AGG 0.652681 8_1:-746018 Msa1171140:CDS
TCCTTATGATGATATACACA+AGG 0.655387 8_1:-743690 Msa1171140:three_prime_UTR
TAACTGAAATTGAGAAGCTG+TGG 0.665853 8_1:+746137 None:intergenic
CTTTGGGGTAGATTATGCGA+CGG 0.678356 8_1:-746675 Msa1171140:CDS
TTGCTGTTGAAGTACAGACA+GGG 0.681711 8_1:+746791 None:intergenic
GGTTTGATCAATATGTTACG+GGG 0.698782 8_1:-745984 Msa1171140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CCATTTTTTAAAATTATTAT+CGG - chr8_1:743936-743955 Msa1171140:intron 10.0%
!!! TTTTATTTATATTCAATTTC+AGG - chr8_1:745745-745764 Msa1171140:CDS 10.0%
!!! TTTTAAAAAATGGCATAATT+TGG + chr8_1:743929-743948 None:intergenic 15.0%
!!! CCGATAATAATTTTAAAAAA+TGG + chr8_1:743939-743958 None:intergenic 15.0%
!! ATTTCATATAATTAGGATTT+AGG + chr8_1:744104-744123 None:intergenic 15.0%
!! TTGTCAAAAGAAATATAATT+AGG - chr8_1:745711-745730 Msa1171140:CDS 15.0%
!! TTCATATTCATAGTAATAAA+GGG + chr8_1:745989-746008 None:intergenic 15.0%
!! ATTCATATTCATAGTAATAA+AGG + chr8_1:745990-746009 None:intergenic 15.0%
!!! TTACTATGAATATGAATTTT+TGG - chr8_1:745993-746012 Msa1171140:CDS 15.0%
!!! ATCATTCTAAAATAACATAT+TGG - chr8_1:746074-746093 Msa1171140:CDS 15.0%
!!! AACTTTTCAAAAATATGTAT+AGG - chr8_1:746263-746282 Msa1171140:CDS 15.0%
!! ACCAAATAATCAATTATATA+GGG + chr8_1:747083-747102 None:intergenic 15.0%
!! AACCAAATAATCAATTATAT+AGG + chr8_1:747084-747103 None:intergenic 15.0%
!! AGAAAAAAAAGAATCAAATA+GGG + chr8_1:747735-747754 None:intergenic 15.0%
!! AAGAAAAAAAAGAATCAAAT+AGG + chr8_1:747736-747755 None:intergenic 15.0%
!! AGAAGAAGAAAAAAAGTAAT+GGG + chr8_1:743538-743557 None:intergenic 20.0%
!! AAGAAGAAGAAAAAAAGTAA+TGG + chr8_1:743539-743558 None:intergenic 20.0%
!! ATGAGAATGTATATATACTA+TGG - chr8_1:744043-744062 Msa1171140:intron 20.0%
!!! ATACTATGGTTCTTTTTATA+AGG - chr8_1:744057-744076 Msa1171140:intron 20.0%
!! GATCACTATTTCATATAATT+AGG + chr8_1:744111-744130 None:intergenic 20.0%
!! GCAATTACTAAACTTAAAAA+TGG + chr8_1:744133-744152 None:intergenic 20.0%
!! TTTAATAGTGTATGTACTAT+TGG - chr8_1:744214-744233 Msa1171140:intron 20.0%
!! TTTCTTATATATAGAATCGA+AGG - chr8_1:744319-744338 Msa1171140:intron 20.0%
!! AAGACAAAATATGATGAATA+AGG + chr8_1:744649-744668 None:intergenic 20.0%
!!! TCATCATATTTTGTCTTTTA+TGG - chr8_1:744652-744671 Msa1171140:intron 20.0%
!! CAAAAGAAATATAATTAGGA+TGG - chr8_1:745715-745734 Msa1171140:CDS 20.0%
!! AATGTTTGTCTTATAGTAAA+TGG - chr8_1:745801-745820 Msa1171140:intron 20.0%
!! TGAGATTGTGAATATTTAAA+AGG - chr8_1:745925-745944 Msa1171140:intron 20.0%
!! TAAATTATTTAAGGATGAAC+AGG + chr8_1:745962-745981 None:intergenic 20.0%
!! AAGGGAGAATAAATTATTTA+AGG + chr8_1:745971-745990 None:intergenic 20.0%
!! TGGTTAAAATTTCTAATTAG+TGG - chr8_1:746106-746125 Msa1171140:CDS 20.0%
!! TTCAAAAATATGTATAGGAT+AGG - chr8_1:746268-746287 Msa1171140:CDS 20.0%
!!! AATTTTCGAGTAACAAAATT+GGG + chr8_1:746467-746486 None:intergenic 20.0%
!!! ATTCTTTCCTAAACTATTTT+GGG + chr8_1:746953-746972 None:intergenic 20.0%
!!! ATTTTTTTTACAAGTTCAAG+AGG + chr8_1:747030-747049 None:intergenic 20.0%
!! TTAATGAACTGTAAAACAAT+GGG + chr8_1:747512-747531 None:intergenic 20.0%
!!! TTCTACATTTAACATTTTCT+TGG - chr8_1:747659-747678 Msa1171140:five_prime_UTR 20.0%
!!! TCTACATTTAACATTTTCTT+GGG - chr8_1:747660-747679 Msa1171140:five_prime_UTR 20.0%
!!! GTTTTTGTATTAGATTTTGA+AGG - chr8_1:747756-747775 Msa1171140:five_prime_UTR 20.0%
!!! TTTTCTTGTGTAATGTATTT+TGG + chr8_1:748022-748041 None:intergenic 20.0%
! ATGATAACTTTACTATTCCA+TGG + chr8_1:743599-743618 None:intergenic 25.0%
!! TTTTCCATTAATATGTGAGT+TGG - chr8_1:743692-743711 Msa1171140:three_prime_UTR 25.0%
!!! CTTTGTTTCAATTTTCTTTG+AGG - chr8_1:743718-743737 Msa1171140:three_prime_UTR 25.0%
! GCATAACATTGAATAATTTG+AGG + chr8_1:743853-743872 None:intergenic 25.0%
! TATCAAGATGAGTAATTAAC+CGG + chr8_1:743994-744013 None:intergenic 25.0%
!! AGGAATAGTTAATGTGTTTT+AGG - chr8_1:744077-744096 Msa1171140:intron 25.0%
! TCTAACAACACACAAAAAAA+CGG + chr8_1:744360-744379 None:intergenic 25.0%
!!! TCAATGGATAATTTCTAGTT+TGG - chr8_1:744420-744439 Msa1171140:intron 25.0%
!! TTCTTATTTACTATCGCTTT+GGG - chr8_1:744840-744859 Msa1171140:intron 25.0%
! AAGAAATCAGAATTAATCTC+CGG - chr8_1:744930-744949 Msa1171140:intron 25.0%
! TGTTGTAAAACCAAAAAGTT+CGG + chr8_1:745158-745177 None:intergenic 25.0%
! AAATTGACACAATTAGCAAA+TGG + chr8_1:745241-745260 None:intergenic 25.0%
! ATGGTTTGATCAATATGTTA+CGG - chr8_1:745545-745564 Msa1171140:intron 25.0%
! TTACTATAAGACAAACATTC+TGG + chr8_1:745800-745819 None:intergenic 25.0%
!! TAACATATTGGTCTAATGAT+TGG - chr8_1:746086-746105 Msa1171140:CDS 25.0%
!!! GAATTTTCGAGTAACAAAAT+TGG + chr8_1:746468-746487 None:intergenic 25.0%
!!! TTGAGCTATTTCAATTTTCA+TGG + chr8_1:746823-746842 None:intergenic 25.0%
! GTGTATATATACCTAATCTA+GGG + chr8_1:746929-746948 None:intergenic 25.0%
! TGTGTATATATACCTAATCT+AGG + chr8_1:746930-746949 None:intergenic 25.0%
!! GATTCTTTCCTAAACTATTT+TGG + chr8_1:746954-746973 None:intergenic 25.0%
! TCATATTTCACTGTATAATC+AGG - chr8_1:746996-747015 Msa1171140:CDS 25.0%
! GCCCTATATAATTGATTATT+TGG - chr8_1:747079-747098 Msa1171140:five_prime_UTR 25.0%
!!! TATTTGGTTCTTATGAAATC+TGG - chr8_1:747095-747114 Msa1171140:five_prime_UTR 25.0%
! AATAACCAATTATGAGAGTA+TGG - chr8_1:747135-747154 Msa1171140:five_prime_UTR 25.0%
! ACTTCTATGAAAAAACTGTT+TGG - chr8_1:747298-747317 Msa1171140:intron 25.0%
! CTTAATGAACTGTAAAACAA+TGG + chr8_1:747513-747532 None:intergenic 25.0%
! CAGTTCATTAAGTATTTGTA+CGG - chr8_1:747521-747540 Msa1171140:intron 25.0%
! GTATAATTATTTACATCCAC+AGG + chr8_1:747562-747581 None:intergenic 25.0%
! ACTACAATTATTCACACATA+AGG + chr8_1:747914-747933 None:intergenic 25.0%
!!! ATGTATTTTGGAGACAATTT+TGG + chr8_1:748010-748029 None:intergenic 25.0%
!! TTTCTCTCATGATTCAATTT+TGG + chr8_1:748060-748079 None:intergenic 25.0%
! ATTGAATCATGAGAGAAAAA+CGG - chr8_1:748062-748081 Msa1171140:five_prime_UTR 25.0%
CTATGGAAGATGATGATAAT+GGG + chr8_1:743483-743502 None:intergenic 30.0%
ACTATGGAAGATGATGATAA+TGG + chr8_1:743484-743503 None:intergenic 30.0%
GTTGAAAAAGGAAGAGAATT+TGG + chr8_1:743513-743532 None:intergenic 30.0%
!! AAGTAATGGGTTGTTGAAAA+AGG + chr8_1:743525-743544 None:intergenic 30.0%
TTTGTTGCAAATTTCCTTGA+TGG - chr8_1:743649-743668 Msa1171140:three_prime_UTR 30.0%
TTGTTGCAAATTTCCTTGAT+GGG - chr8_1:743650-743669 Msa1171140:three_prime_UTR 30.0%
GAAACCAACTCACATATTAA+TGG + chr8_1:743699-743718 None:intergenic 30.0%
TTCTGTTTGTTTATGAGGAT+TGG - chr8_1:743742-743761 Msa1171140:three_prime_UTR 30.0%
!! TTTGTTTATGAGGATTGGTT+TGG - chr8_1:743747-743766 Msa1171140:three_prime_UTR 30.0%
CTGATATAACCAATCATTGA+AGG - chr8_1:743825-743844 Msa1171140:intron 30.0%
! ATAATTTGGTGACATGTCAT+CGG + chr8_1:743915-743934 None:intergenic 30.0%
TCATAAGAAAACTTAGCAAC+AGG + chr8_1:744027-744046 None:intergenic 30.0%
GTATGTACTATTGGTATTTC+TGG - chr8_1:744223-744242 Msa1171140:intron 30.0%
TTAACACTTTCCATTGATCT+TGG - chr8_1:744252-744271 Msa1171140:intron 30.0%
TTTGATATGACCAAGATCAA+TGG + chr8_1:744265-744284 None:intergenic 30.0%
! CAAATGCTTTTCATTTGTCT+TGG - chr8_1:744281-744300 Msa1171140:intron 30.0%
TTATCCATTGAATAGCTGAT+TGG + chr8_1:744411-744430 None:intergenic 30.0%
!! ATGGATAATTTCTAGTTTGG+AGG - chr8_1:744423-744442 Msa1171140:intron 30.0%
!! TGGATAATTTCTAGTTTGGA+GGG - chr8_1:744424-744443 Msa1171140:intron 30.0%
!!! GATTCCTGTAAACTAGTTTT+CGG + chr8_1:744521-744540 None:intergenic 30.0%
! AAAACTAGTTTACAGGAATC+TGG - chr8_1:744521-744540 Msa1171140:intron 30.0%
ATATGATGAATAAGGCAATC+TGG + chr8_1:744641-744660 None:intergenic 30.0%
ATTTCAAGAAGTTTGTGTTG+TGG - chr8_1:744783-744802 Msa1171140:intron 30.0%
TTTCAAGAAGTTTGTGTTGT+GGG - chr8_1:744784-744803 Msa1171140:intron 30.0%
! GTTCTTATTTACTATCGCTT+TGG - chr8_1:744839-744858 Msa1171140:intron 30.0%
! TCTTATTTACTATCGCTTTG+GGG - chr8_1:744841-744860 Msa1171140:intron 30.0%
! GGATCAGTATCTTTTGAATA+AGG - chr8_1:744877-744896 Msa1171140:intron 30.0%
ATTGAATATGCAGCATTACA+TGG - chr8_1:744902-744921 Msa1171140:intron 30.0%
AGAAATCAGAATTAATCTCC+GGG - chr8_1:744931-744950 Msa1171140:intron 30.0%
!! TTTTAACTCGAGTCTTTTCA+AGG + chr8_1:745014-745033 None:intergenic 30.0%
! TGCAAATGGATTTGAAAAGT+CGG + chr8_1:745128-745147 None:intergenic 30.0%
! TTTCGATGAGATTTTTCCAA+AGG + chr8_1:745505-745524 None:intergenic 30.0%
ATCTCATCGAAATAAAAGAG+AGG - chr8_1:745513-745532 Msa1171140:intron 30.0%
TGGTTTGATCAATATGTTAC+GGG - chr8_1:745546-745565 Msa1171140:intron 30.0%
!! ATTCAATTTCAGGTTACTTG+TGG - chr8_1:745755-745774 Msa1171140:CDS 30.0%
!! TTCAATTTCAGGTTACTTGT+GGG - chr8_1:745756-745775 Msa1171140:CDS 30.0%
AAATGGAAGTCATTGAACTT+TGG - chr8_1:745818-745837 Msa1171140:intron 30.0%
!!! ATTTGCGTGTATACTTTTTC+CGG - chr8_1:746186-746205 Msa1171140:CDS 30.0%
! AATTTTGGATGTTACCCAAT+AGG - chr8_1:746216-746235 Msa1171140:CDS 30.0%
TTACAAACGTCTATATCAGA+AGG + chr8_1:746490-746509 None:intergenic 30.0%
AATTCGCATTATCTTTCGTA+AGG + chr8_1:746796-746815 None:intergenic 30.0%
TAGCTCAATATGTACTAAGA+AGG - chr8_1:746835-746854 Msa1171140:CDS 30.0%
!!! AGTTTTAGAAACCCTAGATT+AGG - chr8_1:746915-746934 Msa1171140:CDS 30.0%
ATACACACCCAAAATAGTTT+AGG - chr8_1:746943-746962 Msa1171140:CDS 30.0%
GTTTAGGAAAGAATCAAGTA+TGG - chr8_1:746959-746978 Msa1171140:CDS 30.0%
AATGACCATACTCTCATAAT+TGG + chr8_1:747143-747162 None:intergenic 30.0%
TAGCATGAAATAATTGATCC+TGG - chr8_1:747198-747217 Msa1171140:intron 30.0%
TTGATCAATTACTTAAGTCC+AGG + chr8_1:747219-747238 None:intergenic 30.0%
! ACAGTTATGCAAGTATTTTG+GGG + chr8_1:747400-747419 None:intergenic 30.0%
! CACAGTTATGCAAGTATTTT+GGG + chr8_1:747401-747420 None:intergenic 30.0%
!!! TTCTATTTTCTTTAGAGCAG+TGG - chr8_1:747457-747476 Msa1171140:intron 30.0%
AGTGGAAGATGATTAGTATT+TGG - chr8_1:747475-747494 Msa1171140:intron 30.0%
! AGTCACCATGATAACATTTT+TGG - chr8_1:747610-747629 Msa1171140:intron 30.0%
! GTCACCATGATAACATTTTT+GGG - chr8_1:747611-747630 Msa1171140:intron 30.0%
ATCATAAGGATCCAAATGTT+GGG + chr8_1:747831-747850 None:intergenic 30.0%
TCCTTATGATGATATACACA+AGG - chr8_1:747841-747860 Msa1171140:five_prime_UTR 30.0%
TCCTTGTGTATATCATCATA+AGG + chr8_1:747845-747864 None:intergenic 30.0%
TATACACAAGGATTATTGCT+TGG - chr8_1:747853-747872 Msa1171140:five_prime_UTR 30.0%
TTGAATCATGAGAGAAAAAC+GGG - chr8_1:748063-748082 Msa1171140:five_prime_UTR 30.0%
TATCATCATCTTCCATAGTC+AGG - chr8_1:743485-743504 Msa1171140:three_prime_UTR 35.0%
!!! CAAATTTCCTTGATGGGTTT+TGG - chr8_1:743656-743675 Msa1171140:three_prime_UTR 35.0%
AAAAGATCCAAAACCCATCA+AGG + chr8_1:743666-743685 None:intergenic 35.0%
GAGGTTTCTGTTTGTTTATG+AGG - chr8_1:743737-743756 Msa1171140:three_prime_UTR 35.0%
!! TTATGAGGATTGGTTTGGAT+TGG - chr8_1:743752-743771 Msa1171140:three_prime_UTR 35.0%
ATGTTATGCATCAATAGCAC+CGG - chr8_1:743864-743883 Msa1171140:intron 35.0%
! GTGTGTTGTTAGATTGTACA+TGG - chr8_1:744367-744386 Msa1171140:intron 35.0%
TGTTGTTAGATTGTACATGG+TGG - chr8_1:744370-744389 Msa1171140:intron 35.0%
TGAACCAATCAGCTATTCAA+TGG - chr8_1:744404-744423 Msa1171140:intron 35.0%
! AATGCCGAAAACTAGTTTAC+AGG - chr8_1:744514-744533 Msa1171140:intron 35.0%
!! AATCTGGTTCTGTTATGAAG+AGG - chr8_1:744537-744556 Msa1171140:intron 35.0%
GAACTTGTGTTCTATCGAAA+AGG - chr8_1:744693-744712 Msa1171140:intron 35.0%
TCAACAGCAAATCATTCACT+CGG - chr8_1:744753-744772 Msa1171140:intron 35.0%
!! TCCTTTTGGTTGCAAATCAT+TGG - chr8_1:745060-745079 Msa1171140:intron 35.0%
! TCCAATGATTTGCAACCAAA+AGG + chr8_1:745064-745083 None:intergenic 35.0%
GAAACATTGAAAAATGCTCG+AGG + chr8_1:745096-745115 None:intergenic 35.0%
AACATAGGGCAAAGCTATTT+AGG + chr8_1:745195-745214 None:intergenic 35.0%
! TAGCTTTGCCCTATGTTATA+CGG - chr8_1:745198-745217 Msa1171140:intron 35.0%
AGGACTCAAATCTGACTTAA+AGG + chr8_1:745290-745309 None:intergenic 35.0%
TAAGTCAGATTTGAGTCCTA+AGG - chr8_1:745291-745310 Msa1171140:intron 35.0%
GCAGATATCACAAAATCCTT+AGG + chr8_1:745310-745329 None:intergenic 35.0%
CTACGATCTTACATCTAAAG+CGG + chr8_1:745366-745385 None:intergenic 35.0%
TAACTGAAATTGAGAAGCTG+TGG + chr8_1:745397-745416 None:intergenic 35.0%
AGGAAGACTAAACTCAAAGA+AGG + chr8_1:745440-745459 None:intergenic 35.0%
! TGAGTTTAGTCTTCCTTCTT+TGG - chr8_1:745444-745463 Msa1171140:intron 35.0%
! TTTAGTCTTCCTTCTTTGGA+TGG - chr8_1:745448-745467 Msa1171140:intron 35.0%
ATGATCAATCCATCCAAAGA+AGG + chr8_1:745460-745479 None:intergenic 35.0%
TCATGAATTACACGAACCTT+TGG - chr8_1:745486-745505 Msa1171140:intron 35.0%
AAAAGAGAGGAGACTTTACA+TGG - chr8_1:745526-745545 Msa1171140:intron 35.0%
GGTTTGATCAATATGTTACG+GGG - chr8_1:745547-745566 Msa1171140:intron 35.0%
!! TCAATTTCAGGTTACTTGTG+GGG - chr8_1:745757-745776 Msa1171140:CDS 35.0%
!!! CAAACATTCTGGCTTTTCTA+AGG + chr8_1:745789-745808 None:intergenic 35.0%
!! AAGTCATTGAACTTTGGAGT+GGG - chr8_1:745824-745843 Msa1171140:intron 35.0%
! TTTTCCGGACATGAGAATTT+TGG - chr8_1:746201-746220 Msa1171140:CDS 35.0%
ACATCCAAAATTCTCATGTC+CGG + chr8_1:746208-746227 None:intergenic 35.0%
! TAACCTATACATCTTGCTGT+GGG + chr8_1:746377-746396 None:intergenic 35.0%
ATAACCTATACATCTTGCTG+TGG + chr8_1:746378-746397 None:intergenic 35.0%
TGCTATGGGATGAATATTTC+AGG + chr8_1:746534-746553 None:intergenic 35.0%
ATAAGGTGAGACATTGCTAT+GGG + chr8_1:746548-746567 None:intergenic 35.0%
TATAAGGTGAGACATTGCTA+TGG + chr8_1:746549-746568 None:intergenic 35.0%
TCTCACCTTATACAAGACTA+AGG - chr8_1:746557-746576 Msa1171140:CDS 35.0%
CTCACCTTATACAAGACTAA+GGG - chr8_1:746558-746577 Msa1171140:CDS 35.0%
!! GTATCCCTTAGTCTTGTATA+AGG + chr8_1:746565-746584 None:intergenic 35.0%
TCAATCTTTCCCAACAGATA+GGG - chr8_1:746586-746605 Msa1171140:CDS 35.0%
ATTATAATCAGTGTCGTGTC+CGG + chr8_1:746639-746658 None:intergenic 35.0%
TTGAATTTAACCACGTGTGT+CGG - chr8_1:746673-746692 Msa1171140:CDS 35.0%
GCACTAACACTTCAATTTGA+AGG + chr8_1:746733-746752 None:intergenic 35.0%
! TGAAGTGTTAGTGCAACTTA+AGG - chr8_1:746739-746758 Msa1171140:CDS 35.0%
! GAAGTGTTAGTGCAACTTAA+GGG - chr8_1:746740-746759 Msa1171140:CDS 35.0%
AGAACGAACAATGAAAACAC+AGG + chr8_1:746894-746913 None:intergenic 35.0%
! GCACAGTTATGCAAGTATTT+TGG + chr8_1:747402-747421 None:intergenic 35.0%
GAACTGTAAAACAATGGGTA+AGG + chr8_1:747507-747526 None:intergenic 35.0%
TCGACCCAAAAATGTTATCA+TGG + chr8_1:747618-747637 None:intergenic 35.0%
CATCATAAGGATCCAAATGT+TGG + chr8_1:747832-747851 None:intergenic 35.0%
ACAAGGATTATTGCTTGGTA+TGG - chr8_1:747858-747877 Msa1171140:five_prime_UTR 35.0%
ATTGCTTGGTATGGTTTGAA+TGG - chr8_1:747867-747886 Msa1171140:five_prime_UTR 35.0%
TTGCTTGGTATGGTTTGAAT+GGG - chr8_1:747868-747887 Msa1171140:five_prime_UTR 35.0%
GGTTTGAATGGGAAATGATT+CGG - chr8_1:747879-747898 Msa1171140:five_prime_UTR 35.0%
TGAATCATGAGAGAAAAACG+GGG - chr8_1:748064-748083 Msa1171140:five_prime_UTR 35.0%
GAGAATTTGGTACCTGACTA+TGG + chr8_1:743500-743519 None:intergenic 40.0%
!!! GCTTGTGACTTTTTCAACCA+TGG - chr8_1:743579-743598 Msa1171140:three_prime_UTR 40.0%
!! AGGATTGGTTTGGATTGGAT+TGG - chr8_1:743757-743776 Msa1171140:three_prime_UTR 40.0%
! TTTGAGGCTCCTTCAATGAT+TGG + chr8_1:743837-743856 None:intergenic 40.0%
CAAGATGAGTAATTAACCGG+TGG + chr8_1:743991-744010 None:intergenic 40.0%
GAAAACTTAGCAACAGGAAG+TGG + chr8_1:744021-744040 None:intergenic 40.0%
TTTGGAGGGTAGTAGTTTGA+TGG - chr8_1:744438-744457 Msa1171140:intron 40.0%
TTGGAGGGTAGTAGTTTGAT+GGG - chr8_1:744439-744458 Msa1171140:intron 40.0%
!!! AAACTAGTTTTCGGCATTCC+AGG + chr8_1:744512-744531 None:intergenic 40.0%
GTGTATAATTGTGTTGCGAC+AGG - chr8_1:744590-744609 Msa1171140:intron 40.0%
CTTGTGTTCTATCGAAAAGG+TGG - chr8_1:744696-744715 Msa1171140:intron 40.0%
! CGAAAAGGTGGAGGTATATT+TGG - chr8_1:744708-744727 Msa1171140:intron 40.0%
! TTGCTGTTGAAGTACAGACA+GGG + chr8_1:744743-744762 None:intergenic 40.0%
!! TTTGCTGTTGAAGTACAGAC+AGG + chr8_1:744744-744763 None:intergenic 40.0%
AGAGGCAACGGAATTTCAAT+TGG + chr8_1:744977-744996 None:intergenic 40.0%
TGGCAAGTAAAAAGAGGCAA+CGG + chr8_1:744989-745008 None:intergenic 40.0%
! AAGGACTGGCAAGTAAAAAG+AGG + chr8_1:744995-745014 None:intergenic 40.0%
AAAGGAGAAGAGACGTTTGT+AGG + chr8_1:745046-745065 None:intergenic 40.0%
! ACAAACGTCTCTTCTCCTTT+TGG - chr8_1:745046-745065 Msa1171140:intron 40.0%
AGTTCGGCTAAACTTGCAAA+TGG + chr8_1:745142-745161 None:intergenic 40.0%
! GCAAGTTTAGCCGAACTTTT+TGG - chr8_1:745145-745164 Msa1171140:intron 40.0%
AGCTTTGCCCTATGTTATAC+GGG - chr8_1:745199-745218 Msa1171140:intron 40.0%
CTCCATTCCCGTATAACATA+GGG + chr8_1:745209-745228 None:intergenic 40.0%
AGACTCAAGTTACTGAGTTG+CGG + chr8_1:745334-745353 None:intergenic 40.0%
! CTAAACTCAAAGAAGGCACA+AGG + chr8_1:745433-745452 None:intergenic 40.0%
TATGTTACGGGGACATCATA+AGG - chr8_1:745558-745577 Msa1171140:intron 40.0%
ACTATCCTTCTGTACAGTTG+CGG - chr8_1:745594-745613 Msa1171140:intron 40.0%
! GAAGTCATTGAACTTTGGAG+TGG - chr8_1:745823-745842 Msa1171140:intron 40.0%
!! ACCTGACTTTGAAGCCTATT+GGG + chr8_1:746233-746252 None:intergenic 40.0%
!! AACCTGACTTTGAAGCCTAT+TGG + chr8_1:746234-746253 None:intergenic 40.0%
ATACCCACAGCAAGATGTAT+AGG - chr8_1:746371-746390 Msa1171140:CDS 40.0%
GGGATGAATATTTCAGGAAG+AGG + chr8_1:746528-746547 None:intergenic 40.0%
CTATCTGTTGGGAAAGATTG+AGG + chr8_1:746587-746606 None:intergenic 40.0%
CTCAATCTTTCCCAACAGAT+AGG - chr8_1:746585-746604 Msa1171140:CDS 40.0%
! ATAGGGTATCCCTATCTGTT+GGG + chr8_1:746598-746617 None:intergenic 40.0%
TACCCTATGAAGCACAAACA+TGG - chr8_1:746610-746629 Msa1171140:CDS 40.0%
GTCCATGTTTGTGCTTCATA+GGG + chr8_1:746615-746634 None:intergenic 40.0%
TGTCCATGTTTGTGCTTCAT+AGG + chr8_1:746616-746635 None:intergenic 40.0%
CTCACCAGATGCTTAACTAA+GGG - chr8_1:746767-746786 Msa1171140:CDS 40.0%
GTAACCCTTAGTTAAGCATC+TGG + chr8_1:746774-746793 None:intergenic 40.0%
TGAAGGATACTTTGCACAGA+AGG - chr8_1:747773-747792 Msa1171140:five_prime_UTR 40.0%
GATACTTTGCACAGAAGGTT+TGG - chr8_1:747778-747797 Msa1171140:five_prime_UTR 40.0%
GTGAAGGTTAACCCAACATT+TGG - chr8_1:747817-747836 Msa1171140:five_prime_UTR 40.0%
! TTTGGTGACATGTCATCGGT+CGG + chr8_1:743911-743930 None:intergenic 45.0%
AACCGGTGGTTTATGAAGCA+CGG + chr8_1:743977-743996 None:intergenic 45.0%
TTCAGTGTGTGTTGTGAACC+TGG - chr8_1:744491-744510 Msa1171140:intron 45.0%
GTGTTCTATCGAAAAGGTGG+AGG - chr8_1:744699-744718 Msa1171140:intron 45.0%
CAGCAAATCATTCACTCGGT+TGG - chr8_1:744757-744776 Msa1171140:intron 45.0%
!! CTTTGGGGTAGATTATGCGA+CGG - chr8_1:744856-744875 Msa1171140:intron 45.0%
GGCAACGGAATTTCAATTGG+TGG + chr8_1:744974-744993 None:intergenic 45.0%
!! ACTCGAGTCTTTTCAAGGAC+TGG + chr8_1:745009-745028 None:intergenic 45.0%
GAGAAGAGACGTTTGTAGGT+TGG + chr8_1:745042-745061 None:intergenic 45.0%
TGGATTTGAAAAGTCGGCTG+CGG + chr8_1:745122-745141 None:intergenic 45.0%
TGCCCTATGTTATACGGGAA+TGG - chr8_1:745204-745223 Msa1171140:intron 45.0%
GCTCCATTCCCGTATAACAT+AGG + chr8_1:745210-745229 None:intergenic 45.0%
CTATACCGCAACTGTACAGA+AGG + chr8_1:745602-745621 None:intergenic 45.0%
TGCGGAAGTAGGCAGTTATA+TGG + chr8_1:745883-745902 None:intergenic 45.0%
CTACATGCTGAGAGCAATTG+CGG + chr8_1:745901-745920 None:intergenic 45.0%
ACCCAATAGGCTTCAAAGTC+AGG - chr8_1:746229-746248 Msa1171140:CDS 45.0%
! CATAGGGTATCCCTATCTGT+TGG + chr8_1:746599-746618 None:intergenic 45.0%
TGAAGCACAAACATGGACAC+CGG - chr8_1:746617-746636 Msa1171140:CDS 45.0%
CCTTAGTTAAGCATCTGGTG+AGG + chr8_1:746769-746788 None:intergenic 45.0%
CCTCACCAGATGCTTAACTA+AGG - chr8_1:746766-746785 Msa1171140:CDS 45.0%
! GTACTTTTATGTCGTGCCTG+TGG - chr8_1:747543-747562 Msa1171140:intron 45.0%
! GTTTGGCTTATGCATTGCTG+AGG - chr8_1:747795-747814 Msa1171140:five_prime_UTR 45.0%
CTTATGCATTGCTGAGGTGA+AGG - chr8_1:747801-747820 Msa1171140:five_prime_UTR 45.0%
ATGAGAGAAAAACGGGGTAC+TGG - chr8_1:748070-748089 Msa1171140:five_prime_UTR 45.0%
!!! TTTTTTAATTTTTTATTTGT+TGG - chr8_1:743557-743576 Msa1171140:three_prime_UTR 5.0%
! ACACCTTTGATCGGAAGTGC+CGG + chr8_1:743886-743905 None:intergenic 50.0%
GTCCGTGCTTCATAAACCAC+CGG - chr8_1:743972-743991 Msa1171140:intron 50.0%
! TTCAATTGGTGGACAGCCAG+AGG + chr8_1:744963-744982 None:intergenic 50.0%
TTTGAAAAGTCGGCTGCGGT+AGG + chr8_1:745118-745137 None:intergenic 50.0%
! TTATACGGGAATGGAGCGTC+TGG - chr8_1:745213-745232 Msa1171140:intron 50.0%
! CTCAAAGAAGGCACAAGGTG+TGG + chr8_1:745428-745447 None:intergenic 50.0%
CTGAGAGCAATTGCGGAAGT+AGG + chr8_1:745894-745913 None:intergenic 50.0%
CGGTCGGACACACCTTTGAT+CGG + chr8_1:743895-743914 None:intergenic 55.0%
TTGTGTTGCGACAGGACACC+AGG - chr8_1:744598-744617 Msa1171140:intron 55.0%
AATCTCCGGGCCAAAACCTC+TGG - chr8_1:744944-744963 Msa1171140:intron 55.0%
!! TGGTGGACAGCCAGAGGTTT+TGG + chr8_1:744957-744976 None:intergenic 55.0%
GTGTCTAACACCGACACACG+TGG + chr8_1:746686-746705 None:intergenic 55.0%
!! TCGGTGTTAGACACTGCACG+TGG - chr8_1:746692-746711 Msa1171140:CDS 55.0%
GTGTTAGACACTGCACGTGG+TGG - chr8_1:746695-746714 Msa1171140:CDS 55.0%
! GCATGCACGCGCGTTGACAA+CGG + chr8_1:743461-743480 None:intergenic 60.0%
! GCACCGGCACTTCCGATCAA+AGG - chr8_1:743880-743899 Msa1171140:intron 60.0%
TTGCGACAGGACACCAGGAC+AGG - chr8_1:744603-744622 Msa1171140:intron 60.0%
ACAGGACACCAGGACAGGCT+AGG - chr8_1:744608-744627 Msa1171140:intron 60.0%
GCATGTCACCTAGCCTGTCC+TGG + chr8_1:744619-744638 None:intergenic 60.0%
!! GACAGCCAGAGGTTTTGGCC+CGG + chr8_1:744952-744971 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 743448 748105 743448 ID=Msa1171140;Name=Msa1171140
chr8_1 mRNA 743448 748105 743448 ID=Msa1171140-mRNA-1;Parent=Msa1171140;Name=Msa1171140-mRNA-1;_AED=0.03;_eAED=0.03;_QI=590|1|1|1|0.33|0|4|340|425
chr8_1 exon 743448 743784 743448 ID=Msa1171140-mRNA-1:exon:198;Parent=Msa1171140-mRNA-1
chr8_1 exon 745607 745786 745607 ID=Msa1171140-mRNA-1:exon:197;Parent=Msa1171140-mRNA-1
chr8_1 exon 745938 747174 745938 ID=Msa1171140-mRNA-1:exon:196;Parent=Msa1171140-mRNA-1
chr8_1 exon 747652 748105 747652 ID=Msa1171140-mRNA-1:exon:195;Parent=Msa1171140-mRNA-1
chr8_1 five_prime_UTR 747652 748105 747652 ID=Msa1171140-mRNA-1:five_prime_utr;Parent=Msa1171140-mRNA-1
chr8_1 five_prime_UTR 747039 747174 747039 ID=Msa1171140-mRNA-1:five_prime_utr;Parent=Msa1171140-mRNA-1
chr8_1 CDS 745938 747038 745938 ID=Msa1171140-mRNA-1:cds;Parent=Msa1171140-mRNA-1
chr8_1 CDS 745610 745786 745610 ID=Msa1171140-mRNA-1:cds;Parent=Msa1171140-mRNA-1
chr8_1 three_prime_UTR 745607 745609 745607 ID=Msa1171140-mRNA-1:three_prime_utr;Parent=Msa1171140-mRNA-1
chr8_1 three_prime_UTR 743448 743784 743448 ID=Msa1171140-mRNA-1:three_prime_utr;Parent=Msa1171140-mRNA-1
Gene Sequence

>Msa1171140

ATGCCGAAAACTAGTTTACAGGAATCTGGTTCTGTTATGAAGAGGTCTAAAAAGACAACTTTGATTTCTCAACATGTGTATAATTGTGTTGCGACAGGACACCAGGACAGGCTAGGTGACATGCCAGATTGCCTTATTCATCATATTTTGTCTTTTATGGAGACAAGAGATGCAATTCGAACTTGTGTTCTATCGAAAAGGTGGAGGTATATTTGGACATCAATTCCCTGTCTGTACTTCAACAGCAAATCATTCACTCGGTTGGTTGATTTCAAGAAGTTTGTGTTGTGGGTTCTATCTCATCGCGATAATTCTCATGTCAAAGTTCTTATTTACTATCGCTTTGGGGTAGATTATGCGACGGATCAGTATCTTTTGAATAAGGTTATTGAATATGCAGCATTACATGGTGTCGAAGAAATCAGAATTAATCTCCGGGCCAAAACCTCTGGCTGTCCACCAATTGAAATTCCGTTGCCTCTTTTTACTTGCCAGTCCTTGAAAAGACTCGAGTTAAAAGATTGCCAACCTACAAACGTCTCTTCTCCTTTTGGTTGCAAATCATTGGACATTTTGCACCTCGAGCATTTTTCAATGTTTCCTACCGCAGCCGACTTTTCAAATCCATTTGCAAGTTTAGCCGAACTTTTTGGTTTTACAACATTGACAGCTTTGCACCTAAATAGCTTTGCCCTATGTTATACGGGAATGGAGCGTCTGGATCCATTTGCTAATTGTGTCAATTTGAAGATTTTGCAATTAAGTGAAATGTCCTTTAAGTCAGATTTGAGTCCTAAGGATTTTGTGATATCTGCTCCGCAACTCAGTAACTTGAGTCTAATTTGCAACCGCTTTAGATGTAAGATCGTAGTTACTTCACCACAGCTTCTCAATTTCAGTTACTTGTATTCCACACCTTGTGCCTTCTTTGAGTTTAGTCTTCCTTCTTTGGATGGATTGATCATTGATGTTCATGAATTACACGAACCTTTGGAAAAATCTCATCGAAATAAAAGAGAGGAGACTTTACATGGTTTGATCAATATGTTACGGGGACATCATAAGGCTGAAGCTGTTAAACTATCCTTCTGTACAGTTGCGGTTACTTGTGGGGCTGCAGCCTTAGAAAAGCCAGAATGTTTGTCTTATAGTAAATGGAAGTCATTGAACTTTGGAGTGGGATCAACATATAAAATCTTCATCAGCAAACTTGACCATATAACTGCCTACTTCCGCAATTGCTCTCAGCATGTAGACTTTGAGATTGTGAATATTTAA
Protein sequence

>Msa1171140

MPKTSLQESGSVMKRSKKTTLISQHVYNCVATGHQDRLGDMPDCLIHHILSFMETRDAIRTCVLSKRWRYIWTSIPCLYFNSKSFTRLVDFKKFVLWVLSHRDNSHVKVLIYYRFGVDYATDQYLLNKVIEYAALHGVEEIRINLRAKTSGCPPIEIPLPLFTCQSLKRLELKDCQPTNVSSPFGCKSLDILHLEHFSMFPTAADFSNPFASLAELFGFTTLTALHLNSFALCYTGMERLDPFANCVNLKILQLSEMSFKSDLSPKDFVISAPQLSNLSLICNRFRCKIVVTSPQLLNFSYLYSTPCAFFEFSLPSLDGLIIDVHELHEPLEKSHRNKREETLHGLINMLRGHHKAEAVKLSFCTVAVTCGAAALEKPECLSYSKWKSLNFGVGSTYKIFISKLDHITAYFRNCSQHVDFEIVNI*