Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189900 | KEH36453.1 | 77.143 | 105 | 17 | 2 | 1 | 105 | 1 | 98 | 4.04e-22 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189900 | A0A072V541 | 77.143 | 105 | 17 | 2 | 1 | 105 | 1 | 98 | 1.93e-22 | 93.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003080 | Msa0189900 | 0.800650 | 1.304591e-48 | -8.615850e-47 |
| Msa0005890 | Msa0189900 | 0.822419 | 2.393591e-53 | -8.615850e-47 |
| Msa0019900 | Msa0189900 | 0.816080 | 6.650143e-52 | -8.615850e-47 |
| Msa0027820 | Msa0189900 | 0.800254 | 1.570830e-48 | -8.615850e-47 |
| Msa0054690 | Msa0189900 | 0.818311 | 2.094126e-52 | -8.615850e-47 |
| Msa0065020 | Msa0189900 | 0.821001 | 5.092229e-53 | -8.615850e-47 |
| Msa0067330 | Msa0189900 | 0.824010 | 1.017630e-53 | -8.615850e-47 |
| Msa0077560 | Msa0189900 | 0.819176 | 1.332536e-52 | -8.615850e-47 |
| Msa0081520 | Msa0189900 | 0.818836 | 1.592122e-52 | -8.615850e-47 |
| Msa0094780 | Msa0189900 | 0.818950 | 1.499853e-52 | -8.615850e-47 |
| Msa0123070 | Msa0189900 | 0.813401 | 2.607650e-51 | -8.615850e-47 |
| Msa0136650 | Msa0189900 | 0.809080 | 2.258410e-50 | -8.615850e-47 |
| Msa0146650 | Msa0189900 | 0.805769 | 1.137576e-49 | -8.615850e-47 |
| Msa0158860 | Msa0189900 | 0.832445 | 9.417889e-56 | -8.615850e-47 |
| Msa0174450 | Msa0189900 | 0.814604 | 1.415758e-51 | -8.615850e-47 |
| Msa0182190 | Msa0189900 | 0.801548 | 8.550053e-49 | -8.615850e-47 |
| Msa0189900 | Msa0225860 | 0.812953 | 3.271056e-51 | -8.615850e-47 |
| Msa0189900 | Msa0298470 | 0.845735 | 3.373792e-59 | -8.615850e-47 |
| Msa0189900 | Msa0307270 | 0.826557 | 2.540739e-54 | -8.615850e-47 |
| Msa0189900 | Msa0340500 | 0.812990 | 3.209970e-51 | -8.615850e-47 |
| Msa0189900 | Msa0375640 | 0.825318 | 5.003983e-54 | -8.615850e-47 |
| Msa0189900 | Msa0378720 | 0.818367 | 2.034341e-52 | -8.615850e-47 |
| Msa0189900 | Msa0424160 | 0.833125 | 6.381950e-56 | -8.615850e-47 |
| Msa0189900 | Msa0426530 | 0.802610 | 5.169889e-49 | -8.615850e-47 |
| Msa0189900 | Msa0428370 | 0.802296 | 6.002056e-49 | -8.615850e-47 |
| Msa0189900 | Msa0430260 | 0.807449 | 5.026806e-50 | -8.615850e-47 |
| Msa0189900 | Msa0432010 | 0.819429 | 1.166830e-52 | -8.615850e-47 |
| Msa0189900 | Msa0465530 | 0.809709 | 1.654689e-50 | -8.615850e-47 |
| Msa0189900 | Msa0474730 | 0.822960 | 1.791084e-53 | -8.615850e-47 |
| Msa0189900 | Msa0481890 | 0.827889 | 1.219263e-54 | -8.615850e-47 |
| Msa0189900 | Msa0483100 | 0.803000 | 4.294275e-49 | -8.615850e-47 |
| Msa0189900 | Msa0484380 | 0.805516 | 1.285331e-49 | -8.615850e-47 |
| Msa0189900 | Msa0500650 | 0.801124 | 1.044006e-48 | -8.615850e-47 |
| Msa0189900 | Msa0509550 | 0.804575 | 2.022777e-49 | -8.615850e-47 |
| Msa0189900 | Msa0515800 | 0.803037 | 4.220970e-49 | -8.615850e-47 |
| Msa0189900 | Msa0517250 | 0.818355 | 2.046501e-52 | -8.615850e-47 |
| Msa0189900 | Msa0528570 | 0.805462 | 1.319567e-49 | -8.615850e-47 |
| Msa0189900 | Msa0567170 | 0.823863 | 1.101597e-53 | -8.615850e-47 |
| Msa0189900 | Msa0580370 | 0.840113 | 1.057131e-57 | -8.615850e-47 |
| Msa0189900 | Msa0582480 | 0.809641 | 1.711867e-50 | -8.615850e-47 |
| Msa0189900 | Msa0586220 | 0.809144 | 2.187799e-50 | -8.615850e-47 |
| Msa0189900 | Msa0591170 | 0.807494 | 4.918657e-50 | -8.615850e-47 |
| Msa0189900 | Msa0614000 | 0.800498 | 1.401348e-48 | -8.615850e-47 |
| Msa0189900 | Msa0624900 | 0.824713 | 6.952383e-54 | -8.615850e-47 |
| Msa0189900 | Msa0626450 | 0.812264 | 4.626083e-51 | -8.615850e-47 |
| Msa0189900 | Msa0626490 | 0.813659 | 2.288779e-51 | -8.615850e-47 |
| Msa0189900 | Msa0658640 | 0.825664 | 4.144267e-54 | -8.615850e-47 |
| Msa0189900 | Msa0660370 | 0.823799 | 1.140276e-53 | -8.615850e-47 |
| Msa0189900 | Msa0661150 | 0.816445 | 5.510627e-52 | -8.615850e-47 |
| Msa0189900 | Msa0664090 | 0.811564 | 6.574495e-51 | -8.615850e-47 |
| Msa0189900 | Msa0664600 | 0.804759 | 1.851415e-49 | -8.615850e-47 |
| Msa0189900 | Msa0666700 | 0.812116 | 4.983721e-51 | -8.615850e-47 |
| Msa0189900 | Msa0695270 | 0.804637 | 1.963503e-49 | -8.615850e-47 |
| Msa0189900 | Msa0703600 | 0.804945 | 1.692899e-49 | -8.615850e-47 |
| Msa0189900 | Msa0709200 | 0.808157 | 3.555864e-50 | -8.615850e-47 |
| Msa0189900 | Msa0709440 | 0.831759 | 1.391215e-55 | -8.615850e-47 |
| Msa0189900 | Msa0711400 | 0.804242 | 2.372934e-49 | -8.615850e-47 |
| Msa0189900 | Msa0730930 | 0.844110 | 9.260575e-59 | -8.615850e-47 |
| Msa0189900 | Msa0735780 | 0.829196 | 5.894607e-55 | -8.615850e-47 |
| Msa0189900 | Msa0795870 | 0.830291 | 3.190486e-55 | -8.615850e-47 |
| Msa0189900 | Msa0819170 | 0.800315 | 1.526619e-48 | -8.615850e-47 |
| Msa0189900 | Msa0833650 | 0.832805 | 7.664909e-56 | -8.615850e-47 |
| Msa0189900 | Msa0878280 | 0.833176 | 6.197177e-56 | -8.615850e-47 |
| Msa0189900 | Msa0880270 | 0.824119 | 9.590329e-54 | -8.615850e-47 |
| Msa0189900 | Msa0982650 | 0.811387 | 7.180012e-51 | -8.615850e-47 |
| Msa0189900 | Msa1013350 | 0.819800 | 9.600990e-53 | -8.615850e-47 |
| Msa0189900 | Msa1020510 | 0.800708 | 1.269532e-48 | -8.615850e-47 |
| Msa0189900 | Msa1042160 | 0.825553 | 4.403214e-54 | -8.615850e-47 |
| Msa0189900 | Msa1042190 | 0.828749 | 7.562415e-55 | -8.615850e-47 |
| Msa0189900 | Msa1072940 | 0.821215 | 4.545972e-53 | -8.615850e-47 |
| Msa0189900 | Msa1080000 | 0.824853 | 6.445935e-54 | -8.615850e-47 |
| Msa0189900 | Msa1084530 | 0.825853 | 3.736832e-54 | -8.615850e-47 |
| Msa0189900 | Msa1094010 | 0.823175 | 1.595670e-53 | -8.615850e-47 |
| Msa0189900 | Msa1125820 | 0.828605 | 8.194603e-55 | -8.615850e-47 |
| Msa0189900 | Msa1125990 | 0.829680 | 4.494518e-55 | -8.615850e-47 |
| Msa0189900 | Msa1132530 | 0.805101 | 1.570257e-49 | -8.615850e-47 |
| Msa0189900 | Msa1132840 | 0.835411 | 1.704170e-56 | -8.615850e-47 |
| Msa0189900 | Msa1160920 | 0.822339 | 2.497238e-53 | -8.615850e-47 |
| Msa0189900 | Msa1167470 | 0.825204 | 5.325185e-54 | -8.615850e-47 |
| Msa0189900 | Msa1171290 | 0.832916 | 7.192552e-56 | -8.615850e-47 |
| Msa0189900 | Msa1186020 | 0.803872 | 2.833010e-49 | -8.615850e-47 |
| Msa0189900 | Msa1200890 | 0.828627 | 8.093463e-55 | -8.615850e-47 |
| Msa0189900 | Msa1208950 | 0.819953 | 8.856556e-53 | -8.615850e-47 |
| Msa0189900 | Msa1215870 | 0.823796 | 1.141980e-53 | -8.615850e-47 |
| Msa0189900 | Msa1226520 | 0.819562 | 1.088415e-52 | -8.615850e-47 |
| Msa0189900 | Msa1306640 | 0.819100 | 1.386776e-52 | -8.615850e-47 |
| Msa0189900 | Msa1307090 | 0.807997 | 3.845895e-50 | -8.615850e-47 |
| Msa0189900 | Msa1307630 | 0.842665 | 2.250319e-58 | -8.615850e-47 |
| Msa0189900 | Msa1316330 | 0.811383 | 7.196930e-51 | -8.615850e-47 |
| Msa0189900 | Msa1359690 | 0.830499 | 2.837071e-55 | -8.615850e-47 |
| Msa0189900 | Msa1368750 | 0.817637 | 2.974137e-52 | -8.615850e-47 |
| Msa0189900 | Msa1371910 | 0.807933 | 3.968392e-50 | -8.615850e-47 |
| Msa0189900 | Msa1374790 | 0.821746 | 3.426610e-53 | -8.615850e-47 |
| Msa0189900 | Msa1375280 | 0.823295 | 1.495529e-53 | -8.615850e-47 |
| Msa0189900 | Msa1393780 | 0.814050 | 1.876747e-51 | -8.615850e-47 |
| Msa0189900 | Msa1409410 | 0.827006 | 1.985586e-54 | -8.615850e-47 |
| Msa0189900 | Msa1414170 | 0.806874 | 6.653723e-50 | -8.615850e-47 |
| Msa0189900 | Msa1415540 | 0.804330 | 2.275017e-49 | -8.615850e-47 |
| Msa0189900 | Msa1420950 | 0.806514 | 7.925827e-50 | -8.615850e-47 |
| Msa0189900 | Msa1440880 | 0.815654 | 8.277391e-52 | -8.615850e-47 |
| Msa0189900 | Msa1440960 | 0.805239 | 1.469712e-49 | -8.615850e-47 |
| Msa0189900 | Msa1451820 | 0.822667 | 2.095732e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189900 | MtrunA17_Chr2g0279411 | 77.143 | 105 | 17 | 2 | 1 | 105 | 1 | 98 | 3.71e-26 | 93.2 |
| Msa0189900 | MtrunA17_Chr2g0279351 | 45.714 | 105 | 42 | 4 | 1 | 104 | 1 | 91 | 2.21e-15 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCTGTTGCTGCTACTGC+TGG | 0.364799 | 2_1:+5359839 | Msa0189900:CDS |
| GTCTCTCCTTCTTCCATCTC+TGG | 0.367654 | 2_1:+5359779 | Msa0189900:CDS |
| TTTCCTTGGCGACTGTGATT+TGG | 0.378125 | 2_1:-5359681 | None:intergenic |
| GGTGAAGTAGCGGATGGTTC+AGG | 0.402991 | 2_1:-5359752 | None:intergenic |
| GTTGTTGAGTTCTACAACAA+TGG | 0.418465 | 2_1:+5359634 | Msa0189900:CDS |
| TACAACAATGGCTCAATCTC+CGG | 0.448075 | 2_1:+5359646 | Msa0189900:CDS |
| GGAGGAGAATTTCCAGTGGC+AGG | 0.448322 | 2_1:-5359731 | None:intergenic |
| GATGGGGAGATTGCTTTCCT+TGG | 0.456249 | 2_1:-5359695 | None:intergenic |
| CAGGAGAAGACGCCGGTGAT+GGG | 0.475687 | 2_1:-5359712 | None:intergenic |
| GCAGGAGAAGACGCCGGTGA+TGG | 0.484603 | 2_1:-5359713 | None:intergenic |
| ACGGCACTGGAAGCAGGTCC+TGG | 0.494599 | 2_1:-5359818 | None:intergenic |
| GAACATTGCTCTTATGATTG+TGG | 0.500197 | 2_1:+5359604 | Msa0189900:CDS |
| ACAGAGACGGCACTGGAAGC+AGG | 0.508633 | 2_1:-5359824 | None:intergenic |
| GGTCCTGGTGCTTCAGATGG+TGG | 0.509452 | 2_1:-5359803 | None:intergenic |
| AGCAGCAACAGAGACGGCAC+TGG | 0.519764 | 2_1:-5359831 | None:intergenic |
| CCGGCGTCTTCTCCTGCCAC+TGG | 0.519854 | 2_1:+5359719 | Msa0189900:CDS |
| GCAGGTCCTGGTGCTTCAGA+TGG | 0.523119 | 2_1:-5359806 | None:intergenic |
| CTTGGCGACTGTGATTTGGT+TGG | 0.533471 | 2_1:-5359677 | None:intergenic |
| GGTGGACCAGAGATGGAAGA+AGG | 0.548296 | 2_1:-5359785 | None:intergenic |
| AGGAGAAGACGCCGGTGATG+GGG | 0.563301 | 2_1:-5359711 | None:intergenic |
| GAAAGCAATCTCCCCATCAC+CGG | 0.563344 | 2_1:+5359700 | Msa0189900:CDS |
| TGAACCATCCGCTACTTCAC+CGG | 0.570119 | 2_1:+5359754 | Msa0189900:CDS |
| TTCAGATGGTGGACCAGAGA+TGG | 0.580542 | 2_1:-5359792 | None:intergenic |
| ATGGAAGAAGGAGAGACAGC+CGG | 0.582727 | 2_1:-5359773 | None:intergenic |
| AGAGACAGCCGGTGAAGTAG+CGG | 0.584069 | 2_1:-5359762 | None:intergenic |
| CAACCAAATCACAGTCGCCA+AGG | 0.590381 | 2_1:+5359678 | Msa0189900:CDS |
| GATTTGGTTGGCGATGAAGC+CGG | 0.598616 | 2_1:-5359665 | None:intergenic |
| GGTCCACCATCTGAAGCACC+AGG | 0.613375 | 2_1:+5359800 | Msa0189900:CDS |
| AGCAGTAGCAGCAACAGAGA+CGG | 0.613692 | 2_1:-5359837 | None:intergenic |
| GAAGTAGCGGATGGTTCAGG+AGG | 0.667793 | 2_1:-5359749 | None:intergenic |
| ACAGCCGGTGAAGTAGCGGA+TGG | 0.678585 | 2_1:-5359758 | None:intergenic |
| TTCAGGAGGAGAATTTCCAG+TGG | 0.715472 | 2_1:-5359735 | None:intergenic |
| CCAGTGGCAGGAGAAGACGC+CGG | 0.748622 | 2_1:-5359719 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAACATTGCTCTTATGATTG+TGG | + | chr2_1:5359604-5359623 | Msa0189900:CDS | 35.0% | |
| GTTGTTGAGTTCTACAACAA+TGG | + | chr2_1:5359634-5359653 | Msa0189900:CDS | 35.0% | |
| !!! | TGCTATTGTCATTTTCGTAG+CGG | + | chr2_1:5359868-5359887 | Msa0189900:CDS | 35.0% |
| TACAACAATGGCTCAATCTC+CGG | + | chr2_1:5359646-5359665 | Msa0189900:CDS | 40.0% | |
| TTTCCTTGGCGACTGTGATT+TGG | - | chr2_1:5359684-5359703 | None:intergenic | 45.0% | |
| TTCAGGAGGAGAATTTCCAG+TGG | - | chr2_1:5359738-5359757 | None:intergenic | 45.0% | |
| ! | GATTTGGTTGGCGATGAAGC+CGG | - | chr2_1:5359668-5359687 | None:intergenic | 50.0% |
| !! | CTTGGCGACTGTGATTTGGT+TGG | - | chr2_1:5359680-5359699 | None:intergenic | 50.0% |
| CAACCAAATCACAGTCGCCA+AGG | + | chr2_1:5359678-5359697 | Msa0189900:CDS | 50.0% | |
| GATGGGGAGATTGCTTTCCT+TGG | - | chr2_1:5359698-5359717 | None:intergenic | 50.0% | |
| GAAAGCAATCTCCCCATCAC+CGG | + | chr2_1:5359700-5359719 | Msa0189900:CDS | 50.0% | |
| TGAACCATCCGCTACTTCAC+CGG | + | chr2_1:5359754-5359773 | Msa0189900:CDS | 50.0% | |
| ATGGAAGAAGGAGAGACAGC+CGG | - | chr2_1:5359776-5359795 | None:intergenic | 50.0% | |
| GTCTCTCCTTCTTCCATCTC+TGG | + | chr2_1:5359779-5359798 | Msa0189900:CDS | 50.0% | |
| !! | TTCAGATGGTGGACCAGAGA+TGG | - | chr2_1:5359795-5359814 | None:intergenic | 50.0% |
| AGCAGTAGCAGCAACAGAGA+CGG | - | chr2_1:5359840-5359859 | None:intergenic | 50.0% | |
| GGAGGAGAATTTCCAGTGGC+AGG | - | chr2_1:5359734-5359753 | None:intergenic | 55.0% | |
| !! | GAAGTAGCGGATGGTTCAGG+AGG | - | chr2_1:5359752-5359771 | None:intergenic | 55.0% |
| !! | GGTGAAGTAGCGGATGGTTC+AGG | - | chr2_1:5359755-5359774 | None:intergenic | 55.0% |
| AGAGACAGCCGGTGAAGTAG+CGG | - | chr2_1:5359765-5359784 | None:intergenic | 55.0% | |
| GGTGGACCAGAGATGGAAGA+AGG | - | chr2_1:5359788-5359807 | None:intergenic | 55.0% | |
| GTCTCTGTTGCTGCTACTGC+TGG | + | chr2_1:5359839-5359858 | Msa0189900:CDS | 55.0% | |
| AGGAGAAGACGCCGGTGATG+GGG | - | chr2_1:5359714-5359733 | None:intergenic | 60.0% | |
| CAGGAGAAGACGCCGGTGAT+GGG | - | chr2_1:5359715-5359734 | None:intergenic | 60.0% | |
| ! | ACAGCCGGTGAAGTAGCGGA+TGG | - | chr2_1:5359761-5359780 | None:intergenic | 60.0% |
| ! | GGTCCACCATCTGAAGCACC+AGG | + | chr2_1:5359800-5359819 | Msa0189900:CDS | 60.0% |
| GGTCCTGGTGCTTCAGATGG+TGG | - | chr2_1:5359806-5359825 | None:intergenic | 60.0% | |
| GCAGGTCCTGGTGCTTCAGA+TGG | - | chr2_1:5359809-5359828 | None:intergenic | 60.0% | |
| ! | ACAGAGACGGCACTGGAAGC+AGG | - | chr2_1:5359827-5359846 | None:intergenic | 60.0% |
| AGCAGCAACAGAGACGGCAC+TGG | - | chr2_1:5359834-5359853 | None:intergenic | 60.0% | |
| GCAGGAGAAGACGCCGGTGA+TGG | - | chr2_1:5359716-5359735 | None:intergenic | 65.0% | |
| CCAGTGGCAGGAGAAGACGC+CGG | - | chr2_1:5359722-5359741 | None:intergenic | 65.0% | |
| ! | ACGGCACTGGAAGCAGGTCC+TGG | - | chr2_1:5359821-5359840 | None:intergenic | 65.0% |
| CCGGCGTCTTCTCCTGCCAC+TGG | + | chr2_1:5359719-5359738 | Msa0189900:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 5359587 | 5359904 | 5359587 | ID=Msa0189900;Name=Msa0189900 |
| chr2_1 | mRNA | 5359587 | 5359904 | 5359587 | ID=Msa0189900-mRNA-1;Parent=Msa0189900;Name=Msa0189900-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|105 |
| chr2_1 | exon | 5359587 | 5359904 | 5359587 | ID=Msa0189900-mRNA-1:exon:2396;Parent=Msa0189900-mRNA-1 |
| chr2_1 | CDS | 5359587 | 5359904 | 5359587 | ID=Msa0189900-mRNA-1:cds;Parent=Msa0189900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
