AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225150 MtrunA17_Chr2g0324821 97.857 140 3 0 1 140 1 140 2.69e-99 281
Msa0225150 MtrunA17_Chr2g0282661 66.667 144 40 2 2 140 12 152 2.28e-64 193
Msa0225150 MtrunA17_Chr5g0413571 65.441 136 43 2 3 138 4 135 1.43e-57 175
Msa0225150 MtrunA17_Chr8g0365411 54.545 132 23 1 8 139 9 103 1.14e-43 139
Msa0225150 MtrunA17_Chr6g0476691 68.675 83 26 0 57 139 17 99 5.54e-38 124
Msa0225150 MtrunA17_Chr5g0438041 43.609 133 58 6 10 137 5 125 3.46e-22 85.5
Msa0225150 MtrunA17_Chr5g0438061 41.085 129 63 3 10 137 103 219 5.04e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225150 AT2G33845.1 71.739 138 36 1 3 140 48 182 1.98e-68 204
Msa0225150 AT4G28440.1 69.630 135 39 1 5 137 15 149 5.42e-63 190
Msa0225150 AT1G23750.1 60.145 138 51 1 2 139 4 137 1.27e-58 178
Msa0225150 AT1G03810.1 67.442 129 41 1 7 135 14 141 2.94e-58 177
Msa0225150 AT1G10590.2 65.926 135 41 3 5 137 6 137 2.37e-57 175
Msa0225150 AT1G10590.1 65.926 135 41 3 5 137 6 137 2.37e-57 175
Msa0225150 AT1G10590.3 65.926 135 41 3 5 137 20 151 4.17e-57 175
Msa0225150 AT1G23750.2 69.474 95 29 0 45 139 6 100 5.09e-47 147

Find 46 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGTACACGCAATAAAT+TGG 0.237468 2_1:-75215685 None:intergenic
TCCCTGACTTCAAAATTTGC+AGG 0.302899 2_1:-75215391 None:intergenic
AACACGAAGCTTTGAATCTA+TGG 0.308708 2_1:+75213191 Msa0225150:exon
AAAATATTTAACAATATTTA+CGG 0.310420 2_1:-75215662 None:intergenic
AGAGTGTGTCCATTGGTTCC+AGG 0.350948 2_1:-75213267 None:intergenic
CTCATTAGTTGAATATGAAT+TGG 0.360041 2_1:+75215426 Msa0225150:CDS
CTGCTGTGCAGGTTCATATA+TGG 0.396491 2_1:+75215539 Msa0225150:three_prime_UTR
CGCAGAATTAAAGTTGCACC+AGG 0.436054 2_1:-75215292 None:intergenic
CTATGAGGTTGGCAGTTGAT+AGG 0.440060 2_1:+75215344 Msa0225150:CDS
TCCTAACCGCGGTGGTCGAT+CGG 0.440345 2_1:+75213329 Msa0225150:CDS
CACTGTCAGAGTGTGTCCAT+TGG 0.463476 2_1:-75213274 None:intergenic
CGCATTGCTGAATGTCTCAT+TGG 0.467345 2_1:+75213375 Msa0225150:CDS
TGAGGTTGGCAGTTGATAGG+TGG 0.471269 2_1:+75215347 Msa0225150:CDS
CAATTTATTGCGTGTACAGT+TGG 0.492134 2_1:+75215686 Msa0225150:three_prime_UTR
TGAACAATTGACTGCTGTGC+AGG 0.501881 2_1:+75215528 Msa0225150:three_prime_UTR
AAGGTCTATCAGCTGAAACC+TGG 0.503388 2_1:+75213249 Msa0225150:CDS
TGCTAAGATTGACATGTTCA+AGG 0.505137 2_1:+75215318 Msa0225150:CDS
TAACTCTTCGCCGGTGAAGA+CGG 0.507256 2_1:+75213305 Msa0225150:CDS
GAGGTTGGCAGTTGATAGGT+GGG 0.510112 2_1:+75215348 Msa0225150:CDS
GCCGATCGACCACCGCGGTT+AGG 0.518836 2_1:-75213330 None:intergenic
CACAGCAGTCAATTGTTCAA+TGG 0.525303 2_1:-75215524 None:intergenic
CAGCTGAAACCTGGAACCAA+TGG 0.527823 2_1:+75213258 Msa0225150:CDS
AATGAGACATTCAGCAATGC+GGG 0.536511 2_1:-75213373 None:intergenic
TGATAGACCTTCACGAACAC+TGG 0.546991 2_1:-75213237 None:intergenic
CGGTTAGGAACCGTCTTCAC+CGG 0.552526 2_1:-75213315 None:intergenic
GAAGGTTGTTAACTCTTCGC+CGG 0.564066 2_1:+75213296 Msa0225150:CDS
ACATGTTCAAGGGATCTATG+AGG 0.578715 2_1:+75215329 Msa0225150:CDS
GCTAAGATTGACATGTTCAA+GGG 0.579635 2_1:+75215319 Msa0225150:CDS
GTTCAAGGGATCTATGAGGT+TGG 0.593452 2_1:+75215333 Msa0225150:CDS
ATTTAGTCTTCAGATTGCTG+TGG 0.594758 2_1:+75215575 Msa0225150:three_prime_UTR
GGTTGGCAGTTGATAGGTGG+GGG 0.611083 2_1:+75215350 Msa0225150:CDS
AAGTTGCACCAGGGTTCATG+AGG 0.612434 2_1:-75215282 None:intergenic
TTCACTGCTCGCAATGAACA+AGG 0.613603 2_1:+75213420 Msa0225150:CDS
TAACCGCGGTGGTCGATCGG+CGG 0.613647 2_1:+75213332 Msa0225150:CDS
TTCTCTTTGAGCAAATCACT+AGG 0.629960 2_1:+75215709 Msa0225150:three_prime_UTR
AGGTTGGCAGTTGATAGGTG+GGG 0.635745 2_1:+75215349 Msa0225150:CDS
GCGAAAACCAGTGTTCGTGA+AGG 0.643757 2_1:+75213230 Msa0225150:CDS
AGACATTCAGCAATGCGGGA+AGG 0.647720 2_1:-75213369 None:intergenic
TGGACACACTCTGACAGTGA+AGG 0.666395 2_1:+75213278 Msa0225150:CDS
CAATGAGACATTCAGCAATG+CGG 0.676023 2_1:-75213374 None:intergenic
GATCAATTGAAAATTGAACA+AGG 0.679889 2_1:-75213158 None:intergenic
GCAGTGGACCTCATGAACCC+TGG 0.684427 2_1:+75215274 Msa0225150:intron
AAGACGGTTCCTAACCGCGG+TGG 0.697992 2_1:+75213321 Msa0225150:CDS
GTGAAGACGGTTCCTAACCG+CGG 0.698190 2_1:+75213318 Msa0225150:CDS
GCAGAATTAAAGTTGCACCA+GGG 0.713466 2_1:-75215291 None:intergenic
TCACCGCCGATCGACCACCG+CGG 0.798992 2_1:-75213335 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAATCGTTGATTTTATA+TGG + chr2_1:75213598-75213617 Msa0225150:intron 15.0%
!!! ATGTGGTTTTTTATTTAAAA+GGG - chr2_1:75214033-75214052 None:intergenic 15.0%
!!! AATGTGGTTTTTTATTTAAA+AGG - chr2_1:75214034-75214053 None:intergenic 15.0%
!!! TGTGGTTTTTTTTTTTTAAT+AGG + chr2_1:75214282-75214301 Msa0225150:intron 15.0%
!!! GTGGTTTTTTTTTTTTAATA+GGG + chr2_1:75214283-75214302 Msa0225150:intron 15.0%
!!! TGGTTTTTTTTTTTTAATAG+GGG + chr2_1:75214284-75214303 Msa0225150:intron 15.0%
!!! AGTTTAATTCTATTGTTTTA+TGG + chr2_1:75214345-75214364 Msa0225150:intron 15.0%
!!! TTTATGCGTATTTTTTATTG+TGG + chr2_1:75213622-75213641 Msa0225150:intron 20.0%
!!! TGTGGTTTTTTATTTAAAAG+GGG - chr2_1:75214032-75214051 None:intergenic 20.0%
!!! GGTTTTTTTTTTTTAATAGG+GGG + chr2_1:75214285-75214304 Msa0225150:intron 20.0%
!! ATTACATAAAAAGCTTATTC+GGG - chr2_1:75214411-75214430 None:intergenic 20.0%
!! CATTACATAAAAAGCTTATT+CGG - chr2_1:75214412-75214431 None:intergenic 20.0%
!!! GAATAAGCTTTTTATGTAAT+GGG + chr2_1:75214411-75214430 Msa0225150:intron 20.0%
! GATCAATTGAAAATTGAACA+AGG - chr2_1:75213161-75213180 None:intergenic 25.0%
! TGTAATAAGTTCATCATGAT+AGG + chr2_1:75213715-75213734 Msa0225150:intron 25.0%
! ATTGATTATACGAAAACAGT+TGG + chr2_1:75213753-75213772 Msa0225150:intron 25.0%
! TAAGTAATTGTGAGACTATT+TGG + chr2_1:75213818-75213837 Msa0225150:intron 25.0%
! AAGTAATTGTGAGACTATTT+GGG + chr2_1:75213819-75213838 Msa0225150:intron 25.0%
! AATAAGTTGTTTATCCATAC+AGG + chr2_1:75213921-75213940 Msa0225150:intron 25.0%
! ATAAGTTGTTTATCCATACA+GGG + chr2_1:75213922-75213941 Msa0225150:intron 25.0%
!!! GTGGTTTTTTATTTAAAAGG+GGG - chr2_1:75214031-75214050 None:intergenic 25.0%
! AAAATTGAAAGTTGCAAAAG+GGG - chr2_1:75214076-75214095 None:intergenic 25.0%
! CAAAATTGAAAGTTGCAAAA+GGG - chr2_1:75214077-75214096 None:intergenic 25.0%
! TCAAAATTGAAAGTTGCAAA+AGG - chr2_1:75214078-75214097 None:intergenic 25.0%
! TTCAAAACAGTAAAAACAAC+TGG + chr2_1:75214115-75214134 Msa0225150:intron 25.0%
! TCAAAACAGTAAAAACAACT+GGG + chr2_1:75214116-75214135 Msa0225150:intron 25.0%
! GATTTATAGGGAAAAGAATA+TGG + chr2_1:75214161-75214180 Msa0225150:intron 25.0%
!! CGAATAAGCTTTTTATGTAA+TGG + chr2_1:75214410-75214429 Msa0225150:intron 25.0%
! AACAAATACTGTAACACAAT+AGG - chr2_1:75214573-75214592 None:intergenic 25.0%
! AAAAACATAACACATGAGAA+TGG - chr2_1:75214941-75214960 None:intergenic 25.0%
!! TATTTTGCCTTTTCAAACTT+TGG + chr2_1:75215006-75215025 Msa0225150:intron 25.0%
!! ATTTTGCCTTTTCAAACTTT+GGG + chr2_1:75215007-75215026 Msa0225150:intron 25.0%
!!! ATTCATTTACTTTTATGCAG+TGG + chr2_1:75215258-75215277 Msa0225150:intron 25.0%
! CTCATTAGTTGAATATGAAT+TGG + chr2_1:75215426-75215445 Msa0225150:CDS 25.0%
! TTCAATGGTCAAACTAAAAA+TGG - chr2_1:75215512-75215531 None:intergenic 25.0%
! GACTAAAAATGGTATCATTT+AGG - chr2_1:75215772-75215791 None:intergenic 25.0%
! TGTTCCATTTCTAAGCATTT+TGG + chr2_1:75213461-75213480 Msa0225150:intron 30.0%
TAAGAGTTAAGAACTCTTTC+AGG - chr2_1:75213488-75213507 None:intergenic 30.0%
GAAAGAGTTCTTAACTCTTA+AGG + chr2_1:75213488-75213507 Msa0225150:intron 30.0%
ATTATACGAAAACAGTTGGA+GGG + chr2_1:75213757-75213776 Msa0225150:intron 30.0%
AAGACTCGAATGCATTAATA+TGG - chr2_1:75213948-75213967 None:intergenic 30.0%
GAATTGGGTTAAAGTGATAT+TGG + chr2_1:75213983-75214002 Msa0225150:intron 30.0%
AAATTGAAAGTTGCAAAAGG+GGG - chr2_1:75214075-75214094 None:intergenic 30.0%
CAAAACAGTAAAAACAACTG+GGG + chr2_1:75214117-75214136 Msa0225150:intron 30.0%
TGAGCAAGTTTCAGATTTAT+AGG + chr2_1:75214148-75214167 Msa0225150:intron 30.0%
GAGCAAGTTTCAGATTTATA+GGG + chr2_1:75214149-75214168 Msa0225150:intron 30.0%
TCAAAATTCAAAGTTGCGAA+AGG + chr2_1:75214237-75214256 Msa0225150:intron 30.0%
CAAAATTCAAAGTTGCGAAA+GGG + chr2_1:75214238-75214257 Msa0225150:intron 30.0%
AAAATTCAAAGTTGCGAAAG+GGG + chr2_1:75214239-75214258 Msa0225150:intron 30.0%
AATAGAATTAAACTCACTCG+TGG - chr2_1:75214339-75214358 None:intergenic 30.0%
AATAGGTTTAACTGTTCAAC+AGG - chr2_1:75214556-75214575 None:intergenic 30.0%
TACAGTATTTGTTTGACATG+TGG + chr2_1:75214580-75214599 Msa0225150:intron 30.0%
ACAGTATTTGTTTGACATGT+GGG + chr2_1:75214581-75214600 Msa0225150:intron 30.0%
GTGATGTTACTGTGATTTAT+AGG + chr2_1:75214736-75214755 Msa0225150:intron 30.0%
TGATTTATAGGAATCAGTGT+GGG + chr2_1:75214748-75214767 Msa0225150:intron 30.0%
TAGTGATGTTGCATAAATCA+GGG + chr2_1:75214823-75214842 Msa0225150:intron 30.0%
!! AAACTTTGGGTTGGATTTAT+TGG + chr2_1:75215020-75215039 Msa0225150:intron 30.0%
!! CTTTTGTTTCTTGCTTGTTT+TGG + chr2_1:75215191-75215210 Msa0225150:intron 30.0%
TTTGGAAGATGATATCAGTT+TGG + chr2_1:75215209-75215228 Msa0225150:intron 30.0%
! TGAAGCGAATCTTTTGAATT+TGG + chr2_1:75215479-75215498 Msa0225150:three_prime_UTR 30.0%
!! AACACGAAGCTTTGAATCTA+TGG + chr2_1:75213191-75213210 Msa0225150:exon 35.0%
AGGTCCAAAATGCTTAGAAA+TGG - chr2_1:75213468-75213487 None:intergenic 35.0%
! CTCATTGATTGTGTTAGTGT+GGG + chr2_1:75213557-75213576 Msa0225150:intron 35.0%
! TCATTGATTGTGTTAGTGTG+GGG + chr2_1:75213558-75213577 Msa0225150:intron 35.0%
GAACCTGTGAAAATTGCTTA+TGG + chr2_1:75213665-75213684 Msa0225150:intron 35.0%
! ATGCCATAAGCAATTTTCAC+AGG - chr2_1:75213671-75213690 None:intergenic 35.0%
GATTATACGAAAACAGTTGG+AGG + chr2_1:75213756-75213775 Msa0225150:intron 35.0%
! TCGAGTCTTTTTACAAGATG+TGG + chr2_1:75213959-75213978 Msa0225150:intron 35.0%
! CGAGTCTTTTTACAAGATGT+GGG + chr2_1:75213960-75213979 Msa0225150:intron 35.0%
! GAGTCTTTTTACAAGATGTG+GGG + chr2_1:75213961-75213980 Msa0225150:intron 35.0%
! TTTTACAAGATGTGGGGAAT+TGG + chr2_1:75213967-75213986 Msa0225150:intron 35.0%
TTTACAAGATGTGGGGAATT+GGG + chr2_1:75213968-75213987 Msa0225150:intron 35.0%
! GGGTTAAAGTGATATTGGTT+CGG + chr2_1:75213988-75214007 Msa0225150:intron 35.0%
AATTGAAAGTTGCAAAAGGG+GGG - chr2_1:75214074-75214093 None:intergenic 35.0%
TAGGGAAAAGAATATGGCTT+AGG + chr2_1:75214167-75214186 Msa0225150:intron 35.0%
AAAAGAATATGGCTTAGGAC+TGG + chr2_1:75214172-75214191 Msa0225150:intron 35.0%
TGATCTTCTACTGTCTTGAA+TGG + chr2_1:75214200-75214219 Msa0225150:intron 35.0%
AAATTCAAAGTTGCGAAAGG+GGG + chr2_1:75214240-75214259 Msa0225150:intron 35.0%
TTTATGGTTTCTGCCAATGT+TGG + chr2_1:75214361-75214380 Msa0225150:intron 35.0%
TTATGGTTTCTGCCAATGTT+GGG + chr2_1:75214362-75214381 Msa0225150:intron 35.0%
ATCATTACAACACTCTAGCT+CGG - chr2_1:75214488-75214507 None:intergenic 35.0%
AATGAATTTGGGCAACACTA+TGG - chr2_1:75214515-75214534 None:intergenic 35.0%
ATAGTGTTGCCCAAATTCAT+TGG + chr2_1:75214514-75214533 Msa0225150:intron 35.0%
CCATACCATCCAATGAATTT+GGG - chr2_1:75214526-75214545 None:intergenic 35.0%
TCCATACCATCCAATGAATT+TGG - chr2_1:75214527-75214546 None:intergenic 35.0%
CAGTATTTGTTTGACATGTG+GGG + chr2_1:75214582-75214601 Msa0225150:intron 35.0%
GTGTAAAAAGAAGTGGTGAA+AGG + chr2_1:75214608-75214627 Msa0225150:intron 35.0%
AAAGAAGTGGTGAAAGGTTA+TGG + chr2_1:75214614-75214633 Msa0225150:intron 35.0%
! TATGGATGATCTTTGTTGTC+TGG + chr2_1:75214632-75214651 Msa0225150:intron 35.0%
ATGTGCTTGGTTATGATTAG+AGG + chr2_1:75214665-75214684 Msa0225150:intron 35.0%
GTGATTTATAGGAATCAGTG+TGG + chr2_1:75214747-75214766 Msa0225150:intron 35.0%
GATTTATAGGAATCAGTGTG+GGG + chr2_1:75214749-75214768 Msa0225150:intron 35.0%
! GGTTGCTATTGCAAAAGAAT+GGG + chr2_1:75214770-75214789 Msa0225150:intron 35.0%
GTAGTGATGTTGCATAAATC+AGG + chr2_1:75214822-75214841 Msa0225150:intron 35.0%
AGTGATGTTGCATAAATCAG+GGG + chr2_1:75214824-75214843 Msa0225150:intron 35.0%
!! TCTCGTGATGTGTTTTTGTT+TGG + chr2_1:75214899-75214918 Msa0225150:intron 35.0%
GAAAAGGCAAAATATACACG+CGG - chr2_1:75215000-75215019 None:intergenic 35.0%
ATCCAACCCAAAGTTTGAAA+AGG - chr2_1:75215016-75215035 None:intergenic 35.0%
!! TTTGGGTTGGATTTATTGGA+TGG + chr2_1:75215024-75215043 Msa0225150:intron 35.0%
GGTATCAGTGAATTCCTATA+TGG + chr2_1:75215045-75215064 Msa0225150:intron 35.0%
TTCAATCGAAACAGCCATAT+AGG - chr2_1:75215062-75215081 None:intergenic 35.0%
TATTGTAGAGTAAGTTGCAC+TGG + chr2_1:75215108-75215127 Msa0225150:intron 35.0%
GCATTTAGACAAAGCACAAA+AGG - chr2_1:75215172-75215191 None:intergenic 35.0%
TGCTAAGATTGACATGTTCA+AGG + chr2_1:75215318-75215337 Msa0225150:CDS 35.0%
GCTAAGATTGACATGTTCAA+GGG + chr2_1:75215319-75215338 Msa0225150:CDS 35.0%
!!! AACCTGCAAATTTTGAAGTC+AGG + chr2_1:75215389-75215408 Msa0225150:CDS 35.0%
!!! ACCTGCAAATTTTGAAGTCA+GGG + chr2_1:75215390-75215409 Msa0225150:CDS 35.0%
! AGCGAATCTTTTGAATTTGG+AGG + chr2_1:75215482-75215501 Msa0225150:three_prime_UTR 35.0%
! GCGAATCTTTTGAATTTGGA+GGG + chr2_1:75215483-75215502 Msa0225150:three_prime_UTR 35.0%
! CGAATCTTTTGAATTTGGAG+GGG + chr2_1:75215484-75215503 Msa0225150:three_prime_UTR 35.0%
! ATTTAGTCTTCAGATTGCTG+TGG + chr2_1:75215575-75215594 Msa0225150:three_prime_UTR 35.0%
CAACTGTACACGCAATAAAT+TGG - chr2_1:75215688-75215707 None:intergenic 35.0%
CAATTTATTGCGTGTACAGT+TGG + chr2_1:75215686-75215705 Msa0225150:three_prime_UTR 35.0%
TTCTCTTTGAGCAAATCACT+AGG + chr2_1:75215709-75215728 Msa0225150:three_prime_UTR 35.0%
AATGAGACATTCAGCAATGC+GGG - chr2_1:75213376-75213395 None:intergenic 40.0%
CAATGAGACATTCAGCAATG+CGG - chr2_1:75213377-75213396 None:intergenic 40.0%
! GCTCATTGATTGTGTTAGTG+TGG + chr2_1:75213556-75213575 Msa0225150:intron 40.0%
TGTGGCATTGTGAGATTGTT+TGG + chr2_1:75213640-75213659 Msa0225150:intron 40.0%
! AAACAGTTGGAGGGTTTGTT+TGG + chr2_1:75213766-75213785 Msa0225150:intron 40.0%
! AACAGTTGGAGGGTTTGTTT+GGG + chr2_1:75213767-75213786 Msa0225150:intron 40.0%
TGCATTAATATGGCCCTGTA+TGG - chr2_1:75213938-75213957 None:intergenic 40.0%
! GTTCGGCTTAAACACACTTT+TGG + chr2_1:75214005-75214024 Msa0225150:intron 40.0%
AATTCAAAGTTGCGAAAGGG+GGG + chr2_1:75214241-75214260 Msa0225150:intron 40.0%
TGTTGCCCAAATTCATTGGA+TGG + chr2_1:75214518-75214537 Msa0225150:intron 40.0%
CCCAAATTCATTGGATGGTA+TGG + chr2_1:75214523-75214542 Msa0225150:intron 40.0%
! GGGGAAAGTGTAAAAAGAAG+TGG + chr2_1:75214601-75214620 Msa0225150:intron 40.0%
TGCTTGGTTATGATTAGAGG+AGG + chr2_1:75214668-75214687 Msa0225150:intron 40.0%
ATCACATGCAAGGAACTCAA+AGG - chr2_1:75214721-75214740 None:intergenic 40.0%
TCACAGTAACATCACATGCA+AGG - chr2_1:75214731-75214750 None:intergenic 40.0%
! GGGTTGCTATTGCAAAAGAA+TGG + chr2_1:75214769-75214788 Msa0225150:intron 40.0%
TGCTATTGCAAAAGAATGGG+TGG + chr2_1:75214773-75214792 Msa0225150:intron 40.0%
CGCAACAGGACAGAAAAAAA+CGG - chr2_1:75214865-75214884 None:intergenic 40.0%
!!! CGTTTTTTTCTGTCCTGTTG+CGG + chr2_1:75214863-75214882 Msa0225150:intron 40.0%
AAACACATCACGAGATTGAC+AGG - chr2_1:75214894-75214913 None:intergenic 40.0%
! TGCCTTTTCAAACTTTGGGT+TGG + chr2_1:75215011-75215030 Msa0225150:intron 40.0%
ACTCTACAATAGCTTCAGAG+TGG - chr2_1:75215099-75215118 None:intergenic 40.0%
ATATCCGTCGTTCTATTCCT+TGG + chr2_1:75215141-75215160 Msa0225150:intron 40.0%
GCAGAATTAAAGTTGCACCA+GGG - chr2_1:75215294-75215313 None:intergenic 40.0%
ACATGTTCAAGGGATCTATG+AGG + chr2_1:75215329-75215348 Msa0225150:CDS 40.0%
TCCCTGACTTCAAAATTTGC+AGG - chr2_1:75215394-75215413 None:intergenic 40.0%
CACAGCAGTCAATTGTTCAA+TGG - chr2_1:75215527-75215546 None:intergenic 40.0%
TTGCTACCTTCAAAAACGCA+TGG - chr2_1:75215739-75215758 None:intergenic 40.0%
TGATAGACCTTCACGAACAC+TGG - chr2_1:75213240-75213259 None:intergenic 45.0%
AAGGTCTATCAGCTGAAACC+TGG + chr2_1:75213249-75213268 Msa0225150:CDS 45.0%
GAAGGTTGTTAACTCTTCGC+CGG + chr2_1:75213296-75213315 Msa0225150:CDS 45.0%
CGCATTGCTGAATGTCTCAT+TGG + chr2_1:75213375-75213394 Msa0225150:CDS 45.0%
TTCACTGCTCGCAATGAACA+AGG + chr2_1:75213420-75213439 Msa0225150:CDS 45.0%
TGCAAAAGGGGGGAAACTTA+GGG - chr2_1:75214064-75214083 None:intergenic 45.0%
TTGCAAAAGGGGGGAAACTT+AGG - chr2_1:75214065-75214084 None:intergenic 45.0%
AAACTTAGAGGGCTGGAATG+TGG + chr2_1:75214264-75214283 Msa0225150:intron 45.0%
ATCGCAACTTGTGCGAAGTT+AGG + chr2_1:75214312-75214331 Msa0225150:intron 45.0%
TAGGACACTAGTCCCAACAT+TGG - chr2_1:75214377-75214396 None:intergenic 45.0%
!! TATTCGGGAAAGCGTCAGTT+AGG - chr2_1:75214396-75214415 None:intergenic 45.0%
! ACTCTAGCTCGGATGCATAT+GGG - chr2_1:75214477-75214496 None:intergenic 45.0%
! TGGTTTGTGCATGATGTGCT+TGG + chr2_1:75214652-75214671 Msa0225150:intron 45.0%
TTGCAAAAGAATGGGTGGTG+TGG + chr2_1:75214778-75214797 Msa0225150:intron 45.0%
! TGGGTGGTGTGGCTATATTT+TGG + chr2_1:75214789-75214808 Msa0225150:intron 45.0%
!!! TTTTTTCTGTCCTGTTGCGG+TGG + chr2_1:75214866-75214885 Msa0225150:intron 45.0%
TGTGCCAAGGAATAGAACGA+CGG - chr2_1:75215148-75215167 None:intergenic 45.0%
AAGCACAAAAGGATGTGCCA+AGG - chr2_1:75215161-75215180 None:intergenic 45.0%
CGCAGAATTAAAGTTGCACC+AGG - chr2_1:75215295-75215314 None:intergenic 45.0%
GTTCAAGGGATCTATGAGGT+TGG + chr2_1:75215333-75215352 Msa0225150:CDS 45.0%
CTATGAGGTTGGCAGTTGAT+AGG + chr2_1:75215344-75215363 Msa0225150:CDS 45.0%
TGAACAATTGACTGCTGTGC+AGG + chr2_1:75215528-75215547 Msa0225150:three_prime_UTR 45.0%
CTGCTGTGCAGGTTCATATA+TGG + chr2_1:75215539-75215558 Msa0225150:three_prime_UTR 45.0%
! GGCTTTCCATGCGTTTTTGA+AGG + chr2_1:75215730-75215749 Msa0225150:three_prime_UTR 45.0%
!! AAAATATTTAACAATATTTA+CGG - chr2_1:75215665-75215684 None:intergenic 5.0%
GCGAAAACCAGTGTTCGTGA+AGG + chr2_1:75213230-75213249 Msa0225150:CDS 50.0%
CAGCTGAAACCTGGAACCAA+TGG + chr2_1:75213258-75213277 Msa0225150:CDS 50.0%
!! AGAGTGTGTCCATTGGTTCC+AGG - chr2_1:75213270-75213289 None:intergenic 50.0%
CACTGTCAGAGTGTGTCCAT+TGG - chr2_1:75213277-75213296 None:intergenic 50.0%
TGGACACACTCTGACAGTGA+AGG + chr2_1:75213278-75213297 Msa0225150:CDS 50.0%
TAACTCTTCGCCGGTGAAGA+CGG + chr2_1:75213305-75213324 Msa0225150:CDS 50.0%
AGACATTCAGCAATGCGGGA+AGG - chr2_1:75213372-75213391 None:intergenic 50.0%
! GTTGGAGGGTTTGTTTGGGT+TGG + chr2_1:75213771-75213790 Msa0225150:intron 50.0%
AAAAGGGGGGAAACTTAGGG+GGG - chr2_1:75214061-75214080 None:intergenic 50.0%
CAAAAGGGGGGAAACTTAGG+GGG - chr2_1:75214062-75214081 None:intergenic 50.0%
GCAAAAGGGGGGAAACTTAG+GGG - chr2_1:75214063-75214082 None:intergenic 50.0%
CGAAAGGGGGGAAACTTAGA+GGG + chr2_1:75214253-75214272 Msa0225150:intron 50.0%
! CACTCTAGCTCGGATGCATA+TGG - chr2_1:75214478-75214497 None:intergenic 50.0%
GTTGCATAAATCAGGGGCAG+CGG + chr2_1:75214830-75214849 Msa0225150:intron 50.0%
TTGCATAAATCAGGGGCAGC+GGG + chr2_1:75214831-75214850 Msa0225150:intron 50.0%
AAGTTGCACCAGGGTTCATG+AGG - chr2_1:75215285-75215304 None:intergenic 50.0%
!! TGAGGTTGGCAGTTGATAGG+TGG + chr2_1:75215347-75215366 Msa0225150:CDS 50.0%
!! GAGGTTGGCAGTTGATAGGT+GGG + chr2_1:75215348-75215367 Msa0225150:CDS 50.0%
!! AGGTTGGCAGTTGATAGGTG+GGG + chr2_1:75215349-75215368 Msa0225150:CDS 50.0%
CGGTTAGGAACCGTCTTCAC+CGG - chr2_1:75213318-75213337 None:intergenic 55.0%
GTGAAGACGGTTCCTAACCG+CGG + chr2_1:75213318-75213337 Msa0225150:CDS 55.0%
AACTTAGGGGGGCTGGAATG+TGG - chr2_1:75214050-75214069 None:intergenic 55.0%
GCGAAAGGGGGGAAACTTAG+AGG + chr2_1:75214252-75214271 Msa0225150:intron 55.0%
TTGACAGGTACCACCGCAAC+AGG - chr2_1:75214879-75214898 None:intergenic 55.0%
!! GGTTGGCAGTTGATAGGTGG+GGG + chr2_1:75215350-75215369 Msa0225150:CDS 55.0%
! TGATAGGTGGGGGCGTATAG+AGG + chr2_1:75215360-75215379 Msa0225150:CDS 55.0%
AAGACGGTTCCTAACCGCGG+TGG + chr2_1:75213321-75213340 Msa0225150:CDS 60.0%
TCCTAACCGCGGTGGTCGAT+CGG + chr2_1:75213329-75213348 Msa0225150:CDS 60.0%
AGGGGGGAAACTTAGAGGGC+TGG + chr2_1:75214257-75214276 Msa0225150:intron 60.0%
GCAGTGGACCTCATGAACCC+TGG + chr2_1:75215274-75215293 Msa0225150:intron 60.0%
TAACCGCGGTGGTCGATCGG+CGG + chr2_1:75213332-75213351 Msa0225150:CDS 65.0%
GCCGATCGACCACCGCGGTT+AGG - chr2_1:75213333-75213352 None:intergenic 70.0%
TCACCGCCGATCGACCACCG+CGG - chr2_1:75213338-75213357 None:intergenic 70.0%
GGGGGGAAACTTAGGGGGGC+TGG - chr2_1:75214057-75214076 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75213146 75215799 75213146 ID=Msa0225150;Name=Msa0225150
chr2_1 mRNA 75213146 75215799 75213146 ID=Msa0225150-mRNA-1;Parent=Msa0225150;Name=Msa0225150-mRNA-1;_AED=0.37;_eAED=0.37;_QI=64|1|1|1|1|1|2|331|140
chr2_1 exon 75213146 75213441 75213146 ID=Msa0225150-mRNA-1:exon:22476;Parent=Msa0225150-mRNA-1
chr2_1 exon 75215278 75215799 75215278 ID=Msa0225150-mRNA-1:exon:22477;Parent=Msa0225150-mRNA-1
chr2_1 five_prime_UTR 75213146 75213209 75213146 ID=Msa0225150-mRNA-1:five_prime_utr;Parent=Msa0225150-mRNA-1
chr2_1 CDS 75213210 75213441 75213210 ID=Msa0225150-mRNA-1:cds;Parent=Msa0225150-mRNA-1
chr2_1 CDS 75215278 75215468 75215278 ID=Msa0225150-mRNA-1:cds;Parent=Msa0225150-mRNA-1
chr2_1 three_prime_UTR 75215469 75215799 75215469 ID=Msa0225150-mRNA-1:three_prime_utr;Parent=Msa0225150-mRNA-1
Gene Sequence

>Msa0225150

ATGGCGACACAAACTGCAAAGCGAAAACCAGTGTTCGTGAAGGTCTATCAGCTGAAACCTGGAACCAATGGACACACTCTGACAGTGAAGGTTGTTAACTCTTCGCCGGTGAAGACGGTTCCTAACCGCGGTGGTCGATCGGCGGTGATTGCTTCTCGTCCTTCCCGCATTGCTGAATGTCTCATTGGTGATGAAACTGCTAGCATAATCTTCACTGCTCGCAATGAACAAGTGGACCTCATGAACCCTGGTGCAACTTTAATTCTGCGTAATGCTAAGATTGACATGTTCAAGGGATCTATGAGGTTGGCAGTTGATAGGTGGGGGCGTATAGAGGTCACAGAACCTGCAAATTTTGAAGTCAGGGAAGATAACAATCTCTCATTAGTTGAATATGAATTGGTCAATGTCGTTGAAGAGTAA
Protein sequence

>Msa0225150

MATQTAKRKPVFVKVYQLKPGTNGHTLTVKVVNSSPVKTVPNRGGRSAVIASRPSRIAECLIGDETASIIFTARNEQVDLMNPGATLILRNAKIDMFKGSMRLAVDRWGRIEVTEPANFEVREDNNLSLVEYELVNVVEE*