AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0520790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTGTAGTGGTTGTTTC+TGG 0.049757 3_4:-73318321 None:intergenic
TCTATACTCGAGTTTATATT+TGG 0.177867 3_4:+73320820 Msa0520790:three_prime_UTR
ACCAACACTCGCTCTCTTAT+TGG 0.189980 3_4:+73321162 Msa0520790:three_prime_UTR
AGCTGATTGTTCTGGGAAAT+TGG 0.293695 3_4:+73320722 Msa0520790:CDS
GGTAGAATATTCATCTAATC+AGG 0.301179 3_4:+73321044 Msa0520790:three_prime_UTR
CAGTTACAAGTTTGCATCCT+TGG 0.331082 3_4:+73320682 Msa0520790:intron
CCAAAATACCTGCGGTAAAT+CGG 0.345611 3_4:-73318281 None:intergenic
GTTAATTTGTTGATATGCCT+TGG 0.349561 3_4:+73320968 Msa0520790:three_prime_UTR
ATTAGAAGAACGAGAAATAA+AGG 0.372863 3_4:-73318148 None:intergenic
CCTCTGTAGTGGTTGTTTCT+GGG 0.378764 3_4:-73318320 None:intergenic
CTAATAGCAGCTCATGTTCT+CGG 0.382026 3_4:+73318166 Msa0520790:CDS
TTAATTTGTTGATATGCCTT+GGG 0.382176 3_4:+73320969 Msa0520790:three_prime_UTR
GTTATTTGAGAATAGATGTA+AGG 0.422271 3_4:-73318247 None:intergenic
CCCTGGACGCAATAGAACTT+TGG 0.432262 3_4:-73318008 None:intergenic
ATTGCGTCCAGGGTTCAGTT+GGG 0.433906 3_4:+73318018 Msa0520790:five_prime_UTR
CCTGGACGCAATAGAACTTT+GGG 0.446142 3_4:-73318007 None:intergenic
TATTGCGTCCAGGGTTCAGT+TGG 0.448218 3_4:+73318017 Msa0520790:five_prime_UTR
CCCAAAGTTCTATTGCGTCC+AGG 0.467958 3_4:+73318007 Msa0520790:five_prime_UTR
AGCGAGTGTTGGTGAGATTG+TGG 0.469667 3_4:-73321152 None:intergenic
AGCAGCTCATGTTCTCGGTC+TGG 0.477055 3_4:+73318171 Msa0520790:CDS
CTTGCGCCATTGTACAAAAC+GGG 0.483536 3_4:-73318105 None:intergenic
CGACTCTTATCACTTTATGT+AGG 0.486201 3_4:-73321227 None:intergenic
GCAATAGAACTTTGGGTGGG+TGG 0.487284 3_4:-73318000 None:intergenic
TTTCATTTGTTGAACTGTGA+AGG 0.489687 3_4:+73318043 Msa0520790:five_prime_UTR
CCAAAGTTCTATTGCGTCCA+GGG 0.490923 3_4:+73318008 Msa0520790:five_prime_UTR
GCTCTCTTATTGGATGATAT+CGG 0.497090 3_4:+73321172 Msa0520790:three_prime_UTR
TAACTCACTGCTTAGATGAT+CGG 0.503644 3_4:-73321104 None:intergenic
TCTTGCGCCATTGTACAAAA+CGG 0.509754 3_4:-73318106 None:intergenic
GAGAATAGATGTAAGGGGAA+TGG 0.514696 3_4:-73318240 None:intergenic
TTGTTGAACTGTGAAGGATC+TGG 0.525998 3_4:+73318049 Msa0520790:five_prime_UTR
CAATAGAACTTTGGGTGGGT+GGG 0.540999 3_4:-73317999 None:intergenic
TTATTTGAGAATAGATGTAA+GGG 0.549227 3_4:-73318246 None:intergenic
TCTGGGAAATTGGCAATGCT+CGG 0.550050 3_4:+73320732 Msa0520790:CDS
TGAAAACCCCAACTGAACCC+TGG 0.556509 3_4:-73318025 None:intergenic
TTGCGTCCAGGGTTCAGTTG+GGG 0.562869 3_4:+73318019 Msa0520790:five_prime_UTR
AAATTGGCAATGCTCGGAAG+TGG 0.564571 3_4:+73320738 Msa0520790:CDS
TGAGCCTTTCGCCTCTGTAG+TGG 0.565129 3_4:-73318331 None:intergenic
ACGAGAAATAAAGGAACTGC+CGG 0.582979 3_4:-73318139 None:intergenic
TCCAATAAGAGAGCGAGTGT+TGG 0.598655 3_4:-73321163 None:intergenic
ATTCTTAAACATACAGCCCA+AGG 0.610558 3_4:-73320985 None:intergenic
GGCGCAAGATCAATACTCAC+CGG 0.610616 3_4:+73318120 Msa0520790:CDS
GTAGAATATTCATCTAATCA+GGG 0.617357 3_4:+73321045 Msa0520790:three_prime_UTR
AACAATCTCAGAAGACCTTG+AGG 0.620821 3_4:-73320786 None:intergenic
GGACGCAATAGAACTTTGGG+TGG 0.628816 3_4:-73318004 None:intergenic
GAAAGGCTCACTGAAAGAGA+AGG 0.649077 3_4:+73318344 Msa0520790:CDS
CCCAGAAACAACCACTACAG+AGG 0.669217 3_4:+73318320 Msa0520790:CDS
ACAACCACTACAGAGGCGAA+AGG 0.669954 3_4:+73318327 Msa0520790:CDS
GACGCAATAGAACTTTGGGT+GGG 0.678182 3_4:-73318003 None:intergenic
TATTTGAGAATAGATGTAAG+GGG 0.684162 3_4:-73318245 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACTCAATATTTTTTTT+TGG - chr3_4:73319265-73319284 None:intergenic 10.0%
!!! CTAAAATTGTTATTTATTTA+CGG + chr3_4:73320038-73320057 Msa0520790:intron 10.0%
!!! TAAAATTGTTATTTATTTAC+GGG + chr3_4:73320039-73320058 Msa0520790:intron 10.0%
!!! GATAAATTTTTAAACTTTTA+AGG - chr3_4:73320145-73320164 None:intergenic 10.0%
!!! GTAATTGTTTTCAATTTTAT+AGG + chr3_4:73318403-73318422 Msa0520790:intron 15.0%
!!! TTTGATTTTTCAAAATTGAT+AGG + chr3_4:73318537-73318556 Msa0520790:intron 15.0%
!!! ATAGAAACTAGTAATTTTTT+TGG + chr3_4:73318681-73318700 Msa0520790:intron 15.0%
!!! AAATATTGAGTTTTTCAATT+TGG + chr3_4:73319270-73319289 Msa0520790:intron 15.0%
!!! AATATTGAGTTTTTCAATTT+GGG + chr3_4:73319271-73319290 Msa0520790:intron 15.0%
!! TAAATAAAGTAATTTAACCT+TGG + chr3_4:73320102-73320121 Msa0520790:intron 15.0%
!! TTATTTGAGAATAGATGTAA+GGG - chr3_4:73318249-73318268 None:intergenic 20.0%
!! GATTTGAACTATAATGTAAA+TGG + chr3_4:73318447-73318466 Msa0520790:intron 20.0%
!!! CTAGTTTCTATATATGATTT+TGG - chr3_4:73318672-73318691 None:intergenic 20.0%
!! GTATTTGTAAATAAACTAGT+AGG - chr3_4:73318753-73318772 None:intergenic 20.0%
!! AAAAAATTTCTATTGATGAG+AGG + chr3_4:73319098-73319117 Msa0520790:intron 20.0%
!! AAAAATTTCTATTGATGAGA+GGG + chr3_4:73319099-73319118 Msa0520790:intron 20.0%
!!! ATATTGAGTTTTTCAATTTG+GGG + chr3_4:73319272-73319291 Msa0520790:intron 20.0%
!! AAGTATATAAACCTATCAAT+CGG - chr3_4:73319756-73319775 None:intergenic 20.0%
!!! ACATTTTATTTTATGTCTGT+AGG + chr3_4:73319795-73319814 Msa0520790:intron 20.0%
!!! ACTTCTTTCTTTGTTTATTT+GGG + chr3_4:73320377-73320396 Msa0520790:intron 20.0%
! ATTAGAAGAACGAGAAATAA+AGG - chr3_4:73318151-73318170 None:intergenic 25.0%
! TATTTGAGAATAGATGTAAG+GGG - chr3_4:73318248-73318267 None:intergenic 25.0%
! GTTATTTGAGAATAGATGTA+AGG - chr3_4:73318250-73318269 None:intergenic 25.0%
! TTATAAATCCAAAATACCTG+CGG - chr3_4:73318292-73318311 None:intergenic 25.0%
! GAACTATAATGTAAATGGAT+CGG + chr3_4:73318452-73318471 Msa0520790:intron 25.0%
! GTTTAAATTCATATGTTGCT+CGG + chr3_4:73318481-73318500 Msa0520790:intron 25.0%
!! GTTTCTATATATGATTTTGG+AGG - chr3_4:73318669-73318688 None:intergenic 25.0%
!! ATATGACTCAGGTATATTTT+GGG + chr3_4:73319049-73319068 Msa0520790:intron 25.0%
! ATTCATGGGAGATAAATTTA+TGG + chr3_4:73319138-73319157 Msa0520790:intron 25.0%
! GATATTGCATATTATATGCA+GGG + chr3_4:73319165-73319184 Msa0520790:intron 25.0%
! ATATTGCATATTATATGCAG+GGG + chr3_4:73319166-73319185 Msa0520790:intron 25.0%
!! ACTTTTGAATTGCAAAGAAA+AGG - chr3_4:73319321-73319340 None:intergenic 25.0%
! ACTTCAATTTAACAACATTG+AGG - chr3_4:73319360-73319379 None:intergenic 25.0%
! ATGTTGTTAAATTGAAGTCA+TGG + chr3_4:73319362-73319381 Msa0520790:intron 25.0%
!! CATGGAGATTATTTTCTTTT+AGG - chr3_4:73319543-73319562 None:intergenic 25.0%
! CTAAAAGAAAATAATCTCCA+TGG + chr3_4:73319541-73319560 Msa0520790:intron 25.0%
!! TTTAACTTCTAGACAATCTT+TGG - chr3_4:73319671-73319690 None:intergenic 25.0%
! TTGTCAAAATATCGTTCAAT+TGG + chr3_4:73319711-73319730 Msa0520790:intron 25.0%
! TAATCTAAACTCCCAAAATA+TGG - chr3_4:73319867-73319886 None:intergenic 25.0%
!! CACTTCTTTCTTTGTTTATT+TGG + chr3_4:73320376-73320395 Msa0520790:intron 25.0%
! CAGTTGTTAAATTGAAATCT+AGG + chr3_4:73320607-73320626 Msa0520790:intron 25.0%
! AATGAAATAGATTACTTTGC+TGG - chr3_4:73320655-73320674 None:intergenic 25.0%
!! ACAAAAATTTCTTTTCTCCA+AGG - chr3_4:73320702-73320721 None:intergenic 25.0%
!!! AATTTTGATCATTTTCCTCA+AGG + chr3_4:73320771-73320790 Msa0520790:three_prime_UTR 25.0%
! TCTATACTCGAGTTTATATT+TGG + chr3_4:73320820-73320839 Msa0520790:three_prime_UTR 25.0%
!!! CAACTCAAATTTTGTTTTAC+AGG - chr3_4:73320893-73320912 None:intergenic 25.0%
!!! TTAATTTGTTGATATGCCTT+GGG + chr3_4:73320969-73320988 Msa0520790:three_prime_UTR 25.0%
!!! GTTTTTTGATAGAAGAATAG+TGG + chr3_4:73321023-73321042 Msa0520790:three_prime_UTR 25.0%
! GTAGAATATTCATCTAATCA+GGG + chr3_4:73321045-73321064 Msa0520790:three_prime_UTR 25.0%
! TTTCATTTGTTGAACTGTGA+AGG + chr3_4:73318043-73318062 Msa0520790:five_prime_UTR 30.0%
ACACTGTCTGATATAGATTA+GGG - chr3_4:73318385-73318404 None:intergenic 30.0%
TACACTGTCTGATATAGATT+AGG - chr3_4:73318386-73318405 None:intergenic 30.0%
!!! CTGGTTTATTAGGTTTTAAG+AGG + chr3_4:73318609-73318628 Msa0520790:intron 30.0%
! GAAAGTAAAGTACTCTTACA+GGG + chr3_4:73318703-73318722 Msa0520790:intron 30.0%
CATGCAAACCTAAAATGTAA+GGG - chr3_4:73318889-73318908 None:intergenic 30.0%
AAAATAGCATGATATGACTC+AGG + chr3_4:73319038-73319057 Msa0520790:intron 30.0%
! GATATGACTCAGGTATATTT+TGG + chr3_4:73319048-73319067 Msa0520790:intron 30.0%
TCAATTGACTAGTACATTCA+TGG + chr3_4:73319123-73319142 Msa0520790:intron 30.0%
CAATTGACTAGTACATTCAT+GGG + chr3_4:73319124-73319143 Msa0520790:intron 30.0%
ATGGGAGATAAATTTATGGT+AGG + chr3_4:73319142-73319161 Msa0520790:intron 30.0%
TGGGAGATAAATTTATGGTA+GGG + chr3_4:73319143-73319162 Msa0520790:intron 30.0%
GGATATTGCATATTATATGC+AGG + chr3_4:73319164-73319183 Msa0520790:intron 30.0%
ACAATTAAATTGTGGAGAAG+TGG - chr3_4:73319215-73319234 None:intergenic 30.0%
AGAAAAGGAGAATCATCAAT+TGG - chr3_4:73319306-73319325 None:intergenic 30.0%
CTTTGCAATTCAAAAGTTTG+TGG + chr3_4:73319324-73319343 Msa0520790:intron 30.0%
CTTGAAAAAGTCATAAGTAG+AGG - chr3_4:73319628-73319647 None:intergenic 30.0%
TTGTTAATGATCCGATTGAT+AGG + chr3_4:73319742-73319761 Msa0520790:intron 30.0%
TTATGAATGCACAATTTCCA+AGG - chr3_4:73320122-73320141 None:intergenic 30.0%
ACGAACGGGTATTAAATAAA+GGG - chr3_4:73320244-73320263 None:intergenic 30.0%
! TTCTTTGTTTATTTGGGAGT+AGG + chr3_4:73320383-73320402 Msa0520790:intron 30.0%
AATGCATACAATAGACAATG+TGG - chr3_4:73320538-73320557 None:intergenic 30.0%
!! TTACTTTTGTAGTCCAACTA+GGG + chr3_4:73320574-73320593 Msa0520790:intron 30.0%
! ATTTTTGTAGCTGATTGTTC+TGG + chr3_4:73320714-73320733 Msa0520790:CDS 30.0%
! TTTTTGTAGCTGATTGTTCT+GGG + chr3_4:73320715-73320734 Msa0520790:CDS 30.0%
!!! AGTTGATGAAGTGCATTTTT+AGG + chr3_4:73320908-73320927 Msa0520790:three_prime_UTR 30.0%
!! GTTAATTTGTTGATATGCCT+TGG + chr3_4:73320968-73320987 Msa0520790:three_prime_UTR 30.0%
GGTAGAATATTCATCTAATC+AGG + chr3_4:73321044-73321063 Msa0520790:three_prime_UTR 30.0%
CTGGAAGAAAAAAGAAACAC+TGG + chr3_4:73318068-73318087 Msa0520790:five_prime_UTR 35.0%
AACTACACACGATGAACTAA+AGG - chr3_4:73318564-73318583 None:intergenic 35.0%
AAACCTAATAAACCAGAACG+CGG - chr3_4:73318605-73318624 None:intergenic 35.0%
!!! GGTTTTAAGAGGATCTATGT+AGG + chr3_4:73318620-73318639 Msa0520790:intron 35.0%
! GGAAAGTAAAGTACTCTTAC+AGG + chr3_4:73318702-73318721 Msa0520790:intron 35.0%
! ATCGTTTTCGTAGCTCATAT+AGG + chr3_4:73318807-73318826 Msa0520790:intron 35.0%
!! GAGCATTGAAATTGATGATC+TGG + chr3_4:73318848-73318867 Msa0520790:intron 35.0%
! TTTAGCGTCCCTTACATTTT+AGG + chr3_4:73318878-73318897 Msa0520790:intron 35.0%
CCATGCAAACCTAAAATGTA+AGG - chr3_4:73318890-73318909 None:intergenic 35.0%
!!! CCTTACATTTTAGGTTTGCA+TGG + chr3_4:73318887-73318906 Msa0520790:intron 35.0%
! AGTACGAGCAAGTTTGAAAT+AGG + chr3_4:73318983-73319002 Msa0520790:intron 35.0%
GAGCTCACACAATTAAATTG+TGG - chr3_4:73319223-73319242 None:intergenic 35.0%
!! TTTTTGGTCAAGTAGACTAG+TGG - chr3_4:73319249-73319268 None:intergenic 35.0%
CAAAAGTTTGTGGTTCCTAT+TGG + chr3_4:73319334-73319353 Msa0520790:intron 35.0%
TTAACAACATTGAGGCCAAT+AGG - chr3_4:73319352-73319371 None:intergenic 35.0%
ATTCCTTCACAAACTTCCTA+CGG - chr3_4:73319430-73319449 None:intergenic 35.0%
!!! GTGTTTATATGGCGGATTTT+CGG + chr3_4:73319483-73319502 Msa0520790:intron 35.0%
AAAACCAATGTTGTCAACAG+CGG - chr3_4:73319520-73319539 None:intergenic 35.0%
GCTTTAACAAGATCTGAACT+AGG - chr3_4:73319575-73319594 None:intergenic 35.0%
ATCCACAACTTCGATAAAGA+TGG - chr3_4:73319597-73319616 None:intergenic 35.0%
AGTGCACTATTAACGTTACA+AGG - chr3_4:73319828-73319847 None:intergenic 35.0%
CTAAACTCCCAAAATATGGT+GGG - chr3_4:73319863-73319882 None:intergenic 35.0%
TCTAAACTCCCAAAATATGG+TGG - chr3_4:73319864-73319883 None:intergenic 35.0%
! AATGCTAATTTGTGCCCTTA+GGG - chr3_4:73320220-73320239 None:intergenic 35.0%
! AAATGCTAATTTGTGCCCTT+AGG - chr3_4:73320221-73320240 None:intergenic 35.0%
CACGAACGGGTATTAAATAA+AGG - chr3_4:73320245-73320264 None:intergenic 35.0%
!! CTTACTTTTGTAGTCCAACT+AGG + chr3_4:73320573-73320592 Msa0520790:intron 35.0%
!! TGATGAAGTGCATTTTTAGG+AGG + chr3_4:73320911-73320930 Msa0520790:three_prime_UTR 35.0%
ATTCTTAAACATACAGCCCA+AGG - chr3_4:73320988-73321007 None:intergenic 35.0%
TAACTCACTGCTTAGATGAT+CGG - chr3_4:73321107-73321126 None:intergenic 35.0%
GCTCTCTTATTGGATGATAT+CGG + chr3_4:73321172-73321191 Msa0520790:three_prime_UTR 35.0%
TTGTTGAACTGTGAAGGATC+TGG + chr3_4:73318049-73318068 Msa0520790:five_prime_UTR 40.0%
! CTTCTTCCCGTTTTGTACAA+TGG + chr3_4:73318099-73318118 Msa0520790:exon 40.0%
TCTTGCGCCATTGTACAAAA+CGG - chr3_4:73318109-73318128 None:intergenic 40.0%
ACGAGAAATAAAGGAACTGC+CGG - chr3_4:73318142-73318161 None:intergenic 40.0%
CTAATAGCAGCTCATGTTCT+CGG + chr3_4:73318166-73318185 Msa0520790:CDS 40.0%
TGAAATGAAGTGAGGGAAAG+TGG - chr3_4:73318209-73318228 None:intergenic 40.0%
GGTAGCATGAAATGAAGTGA+GGG - chr3_4:73318216-73318235 None:intergenic 40.0%
TGGTAGCATGAAATGAAGTG+AGG - chr3_4:73318217-73318236 None:intergenic 40.0%
GAGAATAGATGTAAGGGGAA+TGG - chr3_4:73318243-73318262 None:intergenic 40.0%
CCAAAATACCTGCGGTAAAT+CGG - chr3_4:73318284-73318303 None:intergenic 40.0%
AACCTAATAAACCAGAACGC+GGG - chr3_4:73318604-73318623 None:intergenic 40.0%
AATGTAAGGGACGCTAAAGA+TGG - chr3_4:73318876-73318895 None:intergenic 40.0%
GTAGGAAGTTTGTGAAGGAA+TGG + chr3_4:73319429-73319448 Msa0520790:intron 40.0%
TCCGCAATGGTGTGTTTATA+TGG + chr3_4:73319472-73319491 Msa0520790:intron 40.0%
GCCATATAAACACACCATTG+CGG - chr3_4:73319476-73319495 None:intergenic 40.0%
GCAATGGTGTGTTTATATGG+CGG + chr3_4:73319475-73319494 Msa0520790:intron 40.0%
! TGAACTAGGTTGAGTATCCA+TGG - chr3_4:73319561-73319580 None:intergenic 40.0%
AGCCATCTTTATCGAAGTTG+TGG + chr3_4:73319592-73319611 Msa0520790:intron 40.0%
! TCCATTGCCCACCATATTTT+GGG + chr3_4:73319853-73319872 Msa0520790:intron 40.0%
!!! TTTTTCGAGCCTTCTTGAGT+CGG + chr3_4:73319943-73319962 Msa0520790:intron 40.0%
TTCTTATCCTTGTGCCCTTA+GGG - chr3_4:73320080-73320099 None:intergenic 40.0%
TTTCTTATCCTTGTGCCCTT+AGG - chr3_4:73320081-73320100 None:intergenic 40.0%
ATCCTTATATTGCGCCCTAA+GGG + chr3_4:73320203-73320222 Msa0520790:intron 40.0%
AACTGCATTAAACAGCACAC+AGG - chr3_4:73320271-73320290 None:intergenic 40.0%
ATGCAGTTTGTGCCAAACTA+AGG + chr3_4:73320283-73320302 Msa0520790:intron 40.0%
CTCAAGTTAGAGCCTTAGTT+TGG - chr3_4:73320298-73320317 None:intergenic 40.0%
TTTATTTGGGAGTAGGAGTG+TGG + chr3_4:73320390-73320409 Msa0520790:intron 40.0%
! TTATTTGGGAGTAGGAGTGT+GGG + chr3_4:73320391-73320410 Msa0520790:intron 40.0%
! GGTCTGGAGTTAACTAAAGA+TGG - chr3_4:73320506-73320525 None:intergenic 40.0%
TAGACAATGTGGACTTAGAC+TGG - chr3_4:73320527-73320546 None:intergenic 40.0%
CAGTTACAAGTTTGCATCCT+TGG + chr3_4:73320682-73320701 Msa0520790:intron 40.0%
!! AGCTGATTGTTCTGGGAAAT+TGG + chr3_4:73320722-73320741 Msa0520790:CDS 40.0%
AACAATCTCAGAAGACCTTG+AGG - chr3_4:73320789-73320808 None:intergenic 40.0%
! TGTTTTACAGGAAGAACGAC+TGG - chr3_4:73320881-73320900 None:intergenic 40.0%
CAATAGAACTTTGGGTGGGT+GGG - chr3_4:73318002-73318021 None:intergenic 45.0%
GACGCAATAGAACTTTGGGT+GGG - chr3_4:73318006-73318025 None:intergenic 45.0%
CCTGGACGCAATAGAACTTT+GGG - chr3_4:73318010-73318029 None:intergenic 45.0%
CCAAAGTTCTATTGCGTCCA+GGG + chr3_4:73318008-73318027 Msa0520790:five_prime_UTR 45.0%
CTTGCGCCATTGTACAAAAC+GGG - chr3_4:73318108-73318127 None:intergenic 45.0%
AGATGTAAGGGGAATGGAAC+TGG - chr3_4:73318237-73318256 None:intergenic 45.0%
! CCGATTTACCGCAGGTATTT+TGG + chr3_4:73318281-73318300 Msa0520790:intron 45.0%
!! CCTCTGTAGTGGTTGTTTCT+GGG - chr3_4:73318323-73318342 None:intergenic 45.0%
GAAAGGCTCACTGAAAGAGA+AGG + chr3_4:73318344-73318363 Msa0520790:CDS 45.0%
ACCTAATAAACCAGAACGCG+GGG - chr3_4:73318603-73318622 None:intergenic 45.0%
TCTTACAGGGCTCATCAGTT+TGG + chr3_4:73318716-73318735 Msa0520790:intron 45.0%
GAGCTATGTAGCATGCATGA+TGG + chr3_4:73318931-73318950 Msa0520790:intron 45.0%
TGCATATTATATGCAGGGGC+CGG + chr3_4:73319170-73319189 Msa0520790:intron 45.0%
!! GGAATGCCACGATGGATTTT+TGG + chr3_4:73319397-73319416 Msa0520790:intron 45.0%
GGTTGTCCAAAAATCCATCG+TGG - chr3_4:73319406-73319425 None:intergenic 45.0%
!! GATTTTTGGACAACCACCGT+AGG + chr3_4:73319411-73319430 Msa0520790:intron 45.0%
! AAACACACCATTGCGGACTA+TGG - chr3_4:73319469-73319488 None:intergenic 45.0%
! GTCCATTGCCCACCATATTT+TGG + chr3_4:73319852-73319871 Msa0520790:intron 45.0%
!! TCCCAAAATATGGTGGGCAA+TGG - chr3_4:73319857-73319876 None:intergenic 45.0%
GCTCGAAAAATGCACACAAC+AGG - chr3_4:73319933-73319952 None:intergenic 45.0%
TTGAGTCGGCCTTGGAAATA+TGG + chr3_4:73319957-73319976 Msa0520790:intron 45.0%
GTACAGCCACCATATTTCCA+AGG - chr3_4:73319969-73319988 None:intergenic 45.0%
TCTTGCTAACTTCTGCCCTA+AGG + chr3_4:73320062-73320081 Msa0520790:intron 45.0%
CTTGCTAACTTCTGCCCTAA+GGG + chr3_4:73320063-73320082 Msa0520790:intron 45.0%
GATCCTTATATTGCGCCCTA+AGG + chr3_4:73320202-73320221 Msa0520790:intron 45.0%
! AATGTGGACTTAGACTGGTC+TGG - chr3_4:73320522-73320541 None:intergenic 45.0%
TCTGGGAAATTGGCAATGCT+CGG + chr3_4:73320732-73320751 Msa0520790:CDS 45.0%
AAATTGGCAATGCTCGGAAG+TGG + chr3_4:73320738-73320757 Msa0520790:CDS 45.0%
ACCAACACTCGCTCTCTTAT+TGG + chr3_4:73321162-73321181 Msa0520790:three_prime_UTR 45.0%
TCCAATAAGAGAGCGAGTGT+TGG - chr3_4:73321166-73321185 None:intergenic 45.0%
GCAATAGAACTTTGGGTGGG+TGG - chr3_4:73318003-73318022 None:intergenic 50.0%
GGACGCAATAGAACTTTGGG+TGG - chr3_4:73318007-73318026 None:intergenic 50.0%
CCCAAAGTTCTATTGCGTCC+AGG + chr3_4:73318007-73318026 Msa0520790:five_prime_UTR 50.0%
CCCTGGACGCAATAGAACTT+TGG - chr3_4:73318011-73318030 None:intergenic 50.0%
TATTGCGTCCAGGGTTCAGT+TGG + chr3_4:73318017-73318036 Msa0520790:five_prime_UTR 50.0%
ATTGCGTCCAGGGTTCAGTT+GGG + chr3_4:73318018-73318037 Msa0520790:five_prime_UTR 50.0%
TGAAAACCCCAACTGAACCC+TGG - chr3_4:73318028-73318047 None:intergenic 50.0%
GGCGCAAGATCAATACTCAC+CGG + chr3_4:73318120-73318139 Msa0520790:CDS 50.0%
! GTGCTTTTCCGATTTACCGC+AGG + chr3_4:73318273-73318292 Msa0520790:CDS 50.0%
CCCAGAAACAACCACTACAG+AGG + chr3_4:73318320-73318339 Msa0520790:CDS 50.0%
! GCCTCTGTAGTGGTTGTTTC+TGG - chr3_4:73318324-73318343 None:intergenic 50.0%
ACAACCACTACAGAGGCGAA+AGG + chr3_4:73318327-73318346 Msa0520790:CDS 50.0%
GGCTCACTGAAAGAGAAGGT+TGG + chr3_4:73318348-73318367 Msa0520790:intron 50.0%
!! ACCCCGCGTTCTGGTTTATT+AGG + chr3_4:73318599-73318618 Msa0520790:intron 50.0%
GGGTGCGCAATTCTCTAATC+TGG + chr3_4:73319069-73319088 Msa0520790:intron 50.0%
GCATATTATATGCAGGGGCC+GGG + chr3_4:73319171-73319190 Msa0520790:intron 50.0%
CATATTATATGCAGGGGCCG+GGG + chr3_4:73319172-73319191 Msa0520790:intron 50.0%
! TTGTGGAGAAGTGGAGTGTC+CGG - chr3_4:73319206-73319225 None:intergenic 50.0%
CCTTCACAAACTTCCTACGG+TGG - chr3_4:73319427-73319446 None:intergenic 50.0%
CCACCGTAGGAAGTTTGTGA+AGG + chr3_4:73319424-73319443 Msa0520790:intron 50.0%
TTGTGAAGGAATGGCTGCCA+TGG + chr3_4:73319438-73319457 Msa0520790:intron 50.0%
ATGTTGTCAACAGCGGATGG+CGG - chr3_4:73319513-73319532 None:intergenic 50.0%
CCAATGTTGTCAACAGCGGA+TGG - chr3_4:73319516-73319535 None:intergenic 50.0%
CCATCCGCTGTTGACAACAT+TGG + chr3_4:73319513-73319532 Msa0520790:intron 50.0%
! TTTCCAAGGCCGACTCAAGA+AGG - chr3_4:73319955-73319974 None:intergenic 50.0%
AGTCGGCCTTGGAAATATGG+TGG + chr3_4:73319960-73319979 Msa0520790:intron 50.0%
TGCCCTTAGGGCGCAATATA+AGG - chr3_4:73320208-73320227 None:intergenic 50.0%
!! GAAAGAAGTGCTGCGAGTGA+TGG - chr3_4:73320366-73320385 None:intergenic 50.0%
!! AGCGAGTGTTGGTGAGATTG+TGG - chr3_4:73321155-73321174 None:intergenic 50.0%
TTGCGTCCAGGGTTCAGTTG+GGG + chr3_4:73318019-73318038 Msa0520790:five_prime_UTR 55.0%
AGCAGCTCATGTTCTCGGTC+TGG + chr3_4:73318171-73318190 Msa0520790:CDS 55.0%
TGAGCCTTTCGCCTCTGTAG+TGG - chr3_4:73318334-73318353 None:intergenic 55.0%
TGATCTACAACCCCGCGTTC+TGG + chr3_4:73318590-73318609 Msa0520790:intron 55.0%
! TGTGGAGAAGTGGAGTGTCC+GGG - chr3_4:73319205-73319224 None:intergenic 55.0%
!! TTGAAGTCATGGTGCCGCCA+TGG + chr3_4:73319373-73319392 Msa0520790:intron 55.0%
ATCGTGGCATTCCACCATGG+CGG - chr3_4:73319390-73319409 None:intergenic 55.0%
TCCATCGTGGCATTCCACCA+TGG - chr3_4:73319393-73319412 None:intergenic 55.0%
! ACTTCTGCCCTAAGGGCACA+AGG + chr3_4:73320070-73320089 Msa0520790:intron 55.0%
AGCACACAGGATGCACGAAC+GGG - chr3_4:73320258-73320277 None:intergenic 55.0%
CAGCACACAGGATGCACGAA+CGG - chr3_4:73320259-73320278 None:intergenic 55.0%
! GAGTAGGAGTGTGGGTCTCA+TGG + chr3_4:73320399-73320418 Msa0520790:intron 55.0%
CTGATCAGTGCTGCCCTAGT+TGG - chr3_4:73320590-73320609 None:intergenic 55.0%
! GTGGAGAAGTGGAGTGTCCG+GGG - chr3_4:73319204-73319223 None:intergenic 60.0%
!! AAGTCATGGTGCCGCCATGG+TGG + chr3_4:73319376-73319395 Msa0520790:intron 60.0%
! GCCATGGTGGAATGCCACGA+TGG + chr3_4:73319389-73319408 Msa0520790:intron 60.0%
!! TGCGGACTATGGTGCTGCCA+TGG - chr3_4:73319458-73319477 None:intergenic 60.0%
GGCAGCACCATAGTCCGCAA+TGG + chr3_4:73319459-73319478 Msa0520790:intron 60.0%
!! TGTCAACAGCGGATGGCGGA+AGG - chr3_4:73319509-73319528 None:intergenic 60.0%
GAGCCTTCTTGAGTCGGCCT+TGG + chr3_4:73319949-73319968 Msa0520790:intron 60.0%
AGTGTCCGGGGTTCGAGCCC+CGG - chr3_4:73319192-73319211 None:intergenic 70.0%
! AGGGGCCGGGGCTCGAACCC+CGG + chr3_4:73319184-73319203 Msa0520790:intron 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 73317995 73321246 73317995 ID=Msa0520790;Name=Msa0520790
chr3_4 mRNA 73317995 73321246 73317995 ID=Msa0520790-mRNA-1;Parent=Msa0520790;Name=Msa0520790-mRNA-1;_AED=0.13;_eAED=0.13;_QI=123|0.33|0.5|1|0|0.25|4|476|88
chr3_4 exon 73317995 73318192 73317995 ID=Msa0520790-mRNA-1:exon:20537;Parent=Msa0520790-mRNA-1
chr3_4 exon 73318238 73318294 73318238 ID=Msa0520790-mRNA-1:exon:20538;Parent=Msa0520790-mRNA-1
chr3_4 exon 73318317 73318365 73318317 ID=Msa0520790-mRNA-1:exon:20539;Parent=Msa0520790-mRNA-1
chr3_4 exon 73320685 73321246 73320685 ID=Msa0520790-mRNA-1:exon:20540;Parent=Msa0520790-mRNA-1
chr3_4 five_prime_UTR 73317995 73318117 73317995 ID=Msa0520790-mRNA-1:five_prime_utr;Parent=Msa0520790-mRNA-1
chr3_4 CDS 73318118 73318192 73318118 ID=Msa0520790-mRNA-1:cds;Parent=Msa0520790-mRNA-1
chr3_4 CDS 73318238 73318294 73318238 ID=Msa0520790-mRNA-1:cds;Parent=Msa0520790-mRNA-1
chr3_4 CDS 73318317 73318365 73318317 ID=Msa0520790-mRNA-1:cds;Parent=Msa0520790-mRNA-1
chr3_4 CDS 73320685 73320770 73320685 ID=Msa0520790-mRNA-1:cds;Parent=Msa0520790-mRNA-1
chr3_4 three_prime_UTR 73320771 73321246 73320771 ID=Msa0520790-mRNA-1:three_prime_utr;Parent=Msa0520790-mRNA-1
Gene Sequence

>Msa0520790

ATGGCGCAAGATCAATACTCACCGGCAGTTCCTTTATTTCTCGTTCTTCTAATAGCAGCTCATGTTCTCGGTCTGTTCCATTCCCCTTACATCTATTCTCAAATAACTGTGTGCTTTTCCGATTTACCGCAGCTACCCAGAAACAACCACTACAGAGGCGAAAGGCTCACTGAAAGAGAAGTTACAAGTTTGCATCCTTGGAGAAAAGAAATTTTTGTAGCTGATTGTTCTGGGAAATTGGCAATGCTCGGAAGTGGCAAGCTATAA
Protein sequence

>Msa0520790

MAQDQYSPAVPLFLVLLIAAHVLGLFHSPYIYSQITVCFSDLPQLPRNNHYRGERLTEREVTSLHPWRKEIFVADCSGKLAMLGSGKL*