AlfalfaGEDB | Msa0825490

M. sativa cultivar zhongmu-4 / Msa0825490

KEGG: K08101
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0010019 GO:0016491 GO:0016627 GO:0016636 GO:0023052 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050619 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055114 GO:0065007
PFAM: PF05996.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0825490	sp\|Q9SR43\|PFBS_ARATH	56.890	283	122	0	56	338	47	329	7.38e-120	351

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0825490	G7KEL5	96.166	313	8	1	27	339	21	329	0.0	626

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027020	Msa0825490	0.804652	1.949249e-49	-8.615850e-47
Msa0050310	Msa0825490	0.800225	1.592587e-48	-8.615850e-47
Msa0138340	Msa0825490	0.801399	9.172188e-49	-8.615850e-47
Msa0167160	Msa0825490	0.808636	2.810398e-50	-8.615850e-47
Msa0212950	Msa0825490	0.806648	7.427335e-50	-8.615850e-47
Msa0225280	Msa0825490	0.800283	1.549934e-48	-8.615850e-47
Msa0236690	Msa0825490	0.800012	1.759479e-48	-8.615850e-47
Msa0253230	Msa0825490	0.812676	3.760910e-51	-8.615850e-47
Msa0266140	Msa0825490	0.810123	1.347847e-50	-8.615850e-47
Msa0287480	Msa0825490	-0.824160	9.380316e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0825490	MtrunA17_Chr5g0447391	96.166	313	8	1	27	339	21	329	0.0	626

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0825490	AT3G09150.2	56.890	283	122	0	56	338	47	329	7.50e-121	351
Msa0825490	AT3G09150.1	56.184	283	122	1	56	338	47	327	2.17e-117	342
Msa0825490	AT3G09150.3	58.537	246	102	0	93	338	5	250	3.23e-110	321
Msa0825490	AT3G09150.4	55.310	226	101	0	56	281	47	272	2.42e-90	271

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Find 82 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGCACAAAATATG+GGG	0.624106	5_3:-65343487	None:intergenic
AAAGTAACAGTAAAATTTAC+AGG	0.355139	5_3:+65341048	Msa0825490:five_prime_UTR
AAATTATGATATTCTGTATT+CGG	0.324062	5_3:+65344379	Msa0825490:CDS
AACAGTAAAATTTACAGGAA+TGG	0.489912	5_3:+65341053	Msa0825490:five_prime_UTR
AACTTTGTCCAAATGACTAT+CGG	0.570194	5_3:-65344341	None:intergenic
AAGAAGAAGCTGAATTCAAT+GGG	0.503272	5_3:+65341100	Msa0825490:exon
AATAAAATCTAGGAAACTTG+AGG	0.572372	5_3:+65345785	Msa0825490:three_prime_UTR
AATAAAATGAAAACCTGTAA+AGG	0.446441	5_3:-65341358	None:intergenic
AATAAGAAAGGCGGTATTGC+TGG	0.342144	5_3:+65344973	Msa0825490:CDS
AATCGCCCATGGGATGCAAG+CGG	0.651732	5_3:+65345009	Msa0825490:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGAAGCTGAATTCAAT+GGG	+	chr5_3:65341100-65341119	Msa0825490:exon	30.0%
AAGAAGAAGAAGAAGAAGAT+GGG	-	chr5_3:65341156-65341175	None:intergenic	30.0%
TTTAATCCAAACTGAACCAT+GGG	-	chr5_3:65341677-65341696	None:intergenic	30.0%
TCTATATATAGATAGAGCTG+GGG	+	chr5_3:65341918-65341937	Msa0825490:intron	30.0%
AATAAGCTGTTTATCCAAAC+AGG	-	chr5_3:65341944-65341963	None:intergenic	30.0%
CCAAATAAACCAATCTAAAC+AGG	+	chr5_3:65342185-65342204	Msa0825490:intron	30.0%
CACAACAACATTATATCACT+AGG	-	chr5_3:65342415-65342434	None:intergenic	30.0%
TTCCTCACCATATTATTAGT+GGG	+	chr5_3:65342736-65342755	Msa0825490:intron	30.0%
TTTACCATCCTTAGAATTCA+AGG	-	chr5_3:65342816-65342835	None:intergenic	30.0%
CAAATATCTTCTCTATTCTC+CGG	+	chr5_3:65343248-65343267	Msa0825490:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	65341013	65345812	65341013	ID=Msa0825490;Name=Msa0825490
chr5_3	mRNA	65341013	65345812	65341013	ID=Msa0825490-mRNA-1;Parent=Msa0825490;Name=Msa0825490-mRNA-1;_AED=0.09;_eAED=0.09;_QI=105\|1\|1\|1\|0.62\|0.77\|9\|398\|339
chr5_3	exon	65341013	65341366	65341013	ID=Msa0825490-mRNA-1:exon:20474;Parent=Msa0825490-mRNA-1
chr5_3	exon	65342800	65342916	65342800	ID=Msa0825490-mRNA-1:exon:20475;Parent=Msa0825490-mRNA-1
chr5_3	exon	65343446	65343540	65343446	ID=Msa0825490-mRNA-1:exon:20476;Parent=Msa0825490-mRNA-1
chr5_3	exon	65343894	65343987	65343894	ID=Msa0825490-mRNA-1:exon:20477;Parent=Msa0825490-mRNA-1
chr5_3	exon	65344290	65344421	65344290	ID=Msa0825490-mRNA-1:exon:20478;Parent=Msa0825490-mRNA-1
chr5_3	exon	65344496	65344600	65344496	ID=Msa0825490-mRNA-1:exon:20479;Parent=Msa0825490-mRNA-1
chr5_3	exon	65344744	65344794	65344744	ID=Msa0825490-mRNA-1:exon:20480;Parent=Msa0825490-mRNA-1
chr5_3	exon	65344874	65345042	65344874	ID=Msa0825490-mRNA-1:exon:20481;Parent=Msa0825490-mRNA-1
chr5_3	exon	65345407	65345812	65345407	ID=Msa0825490-mRNA-1:exon:20482;Parent=Msa0825490-mRNA-1
chr5_3	five_prime_UTR	65341013	65341117	65341013	ID=Msa0825490-mRNA-1:five_prime_utr;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65341118	65341366	65341118	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65342800	65342916	65342800	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65343446	65343540	65343446	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65343894	65343987	65343894	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65344290	65344421	65344290	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65344496	65344600	65344496	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65344744	65344794	65344744	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65344874	65345042	65344874	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	CDS	65345407	65345414	65345407	ID=Msa0825490-mRNA-1:cds;Parent=Msa0825490-mRNA-1
chr5_3	three_prime_UTR	65345415	65345812	65345415	ID=Msa0825490-mRNA-1:three_prime_utr;Parent=Msa0825490-mRNA-1

Gene Sequence

>Msa0825490

ATGGGTTATAGCGTTTCTTCCTTTTCCTTCTTCTCCCCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCAAGCTTTTCTTTGCAACACACACTTCCACCGTTGTTGAAAAGAACAGCTTCTTCTAACAGTGGTAGCTGCAGAAGAGTTTGCATACAAAGCTGCTTTTCTTCAGTATCTTATCACAAATTTGTTGATTTTGCTTTGGATGAAACCTCTCGCCATACCCACTTGTTCTCTTCCCCTTTACAGGAAAAGTATAATTCCTTGAATTCTAAGGATGGTAAAGGAGTGCTTAATATGTTATCATTTCAAGCTGACAAGATTAGGCTCCTACGTAGTATGATCATTGAGACAGAAACAATGCAGGTTTTGGACTTTACCGTATTTCCAAAAGCGGAGTATGATGTCCCCATATTTTGTGCCAACTTTTTCTCGACCGCCAAAACAAACATTGTTGTGTTGGACCTTAACCCATTACATGATATTATCAATCAGGATGACTACAAGGAAAAGTACTTTAAAAGCTTAATTCCTCTTGGCCTGAAATATGCTGAGCATTTTCCATGGGGAGGGAAGCTCACAAGTGAGTCTATAACTTTTTTTTCACCGATAGTCATTTGGACAAAGTTTACTTCAAGCCCACAAAATTATGATATTCTGTATTCGGCATTCAGAGATTACTACAAGGTATGGTTGGAATTGATAGCCACGGCAGTAAAAGAGACTGATGGATATCAAATTTCCCACAATCTTGAAGCACAACATAGATATCTAACATGGAGAGCCGAAACGGATCCGGGACAAGGTGTTTTGAAGAAGCTGATTGGGGACACACTTGCAAAGGATTTATTAAGAAGCTTTCTCTTTAACGGAGTCGATGAACTAGGAAGCAAAACGTTCAATGATTATTTTCCTCATTACCACGGCGAGGAAGGAATTCTAAATAAGAAAGGCGGTATTGCTGGGAAGTCTTTTGAAAATCGCCCATGGGATGCAAGCGGAAAATTCCTTGGTCAATGA

Protein sequence

>Msa0825490

MGYSVSSFSFFSPSSSSSSSSSSSFSLQHTLPPLLKRTASSNSGSCRRVCIQSCFSSVSYHKFVDFALDETSRHTHLFSSPLQEKYNSLNSKDGKGVLNMLSFQADKIRLLRSMIIETETMQVLDFTVFPKAEYDVPIFCANFFSTAKTNIVVLDLNPLHDIINQDDYKEKYFKSLIPLGLKYAEHFPWGGKLTSESITFFSPIVIWTKFTSSPQNYDILYSAFRDYYKVWLELIATAVKETDGYQISHNLEAQHRYLTWRAETDPGQGVLKKLIGDTLAKDLLRSFLFNGVDELGSKTFNDYFPHYHGEEGILNKKGGIAGKSFENRPWDASGKFLGQ*