AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826820 MtrunA17_Chr5g0448421 98.917 277 3 0 1 277 1 277 0.0 554
Msa0826820 MtrunA17_Chr5g0448411 95.307 277 11 1 1 277 1 275 0.0 531
Msa0826820 MtrunA17_Chr3g0133901 47.436 234 101 9 47 274 45 262 5.24e-61 194
Msa0826820 MtrunA17_Chr8g0345631 42.500 240 112 7 37 274 50 265 6.35e-52 171
Msa0826820 MtrunA17_Chr3g0092091 40.727 275 127 10 9 274 2 249 1.57e-48 161
Msa0826820 MtrunA17_Chr2g0301081 43.111 225 101 7 56 274 61 264 2.28e-48 161
Msa0826820 MtrunA17_Chr4g0051941 37.868 272 131 11 10 269 9 254 1.37e-38 136
Msa0826820 MtrunA17_Chr5g0447531 38.542 288 123 15 1 269 1 253 7.63e-38 134
Msa0826820 MtrunA17_Chr6g0453141 36.466 266 134 9 14 269 13 253 3.45e-37 132
Msa0826820 MtrunA17_Chr6g0453151 41.014 217 93 9 63 269 129 320 5.32e-37 134
Msa0826820 MtrunA17_Chr6g0453181 40.553 217 94 9 63 269 62 253 6.66e-37 132
Msa0826820 MtrunA17_Chr2g0318521 41.475 217 94 8 63 269 59 252 8.47e-37 131
Msa0826820 MtrunA17_Chr6g0462031 41.395 215 93 8 60 270 49 234 1.50e-35 127
Msa0826820 MtrunA17_Chr6g0453161 36.559 279 141 10 1 269 268 520 2.37e-35 132
Msa0826820 MtrunA17_Chr6g0453161 41.014 217 93 9 63 269 64 255 1.12e-34 130
Msa0826820 MtrunA17_Chr6g0476641 38.211 246 114 10 41 274 60 279 1.16e-34 126
Msa0826820 MtrunA17_Chr1g0157481 33.574 277 138 10 26 277 78 333 3.52e-32 121
Msa0826820 MtrunA17_Chr6g0464511 34.146 205 86 6 65 269 45 200 3.60e-26 102
Msa0826820 MtrunA17_Chr2g0279001 35.567 194 91 8 69 247 75 249 6.60e-25 100
Msa0826820 MtrunA17_Chr4g0006181 30.418 263 112 6 55 274 110 344 1.13e-24 101
Msa0826820 MtrunA17_Chr6g0453171 58.108 74 30 1 196 269 14 86 6.96e-23 90.1
Msa0826820 MtrunA17_Chr3g0113311 30.126 239 96 6 59 254 59 269 3.73e-18 82.0
Msa0826820 MtrunA17_Chr2g0296081 53.333 75 30 2 200 274 32 101 1.95e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826820 AT1G61520.3 85.921 277 35 3 1 277 1 273 1.40e-167 465
Msa0826820 AT1G61520.1 85.921 277 35 3 1 277 1 273 1.40e-167 465
Msa0826820 AT1G61520.2 89.545 220 18 1 58 277 4 218 1.36e-144 404
Msa0826820 AT1G45474.2 42.188 256 126 9 23 274 14 251 1.07e-55 180
Msa0826820 AT1G45474.1 42.188 256 126 9 23 274 14 251 1.07e-55 180
Msa0826820 AT3G61470.1 42.500 240 115 7 41 274 32 254 2.97e-50 166
Msa0826820 AT3G47470.1 43.651 252 97 10 30 274 37 250 2.62e-48 161
Msa0826820 AT1G19150.1 38.168 262 138 7 18 277 31 270 3.68e-46 156
Msa0826820 AT3G27690.1 38.028 284 131 14 1 269 1 254 7.72e-39 137
Msa0826820 AT3G27690.2 38.028 284 131 14 1 269 33 286 1.38e-38 137
Msa0826820 AT2G05100.1 38.163 283 131 14 1 269 1 253 1.90e-38 136
Msa0826820 AT2G05070.1 37.809 283 132 14 1 269 1 253 1.37e-37 134
Msa0826820 AT5G54270.1 41.935 217 93 8 63 269 60 253 3.85e-37 132
Msa0826820 AT1G29930.1 38.745 271 131 9 10 269 9 255 6.38e-37 132
Msa0826820 AT1G29920.1 38.745 271 131 9 10 269 9 255 1.66e-36 131
Msa0826820 AT1G29910.1 38.745 271 131 9 10 269 9 255 1.66e-36 131
Msa0826820 AT2G34420.1 37.276 279 139 10 1 269 1 253 6.07e-36 129
Msa0826820 AT2G34430.1 37.903 248 114 10 38 269 31 254 2.49e-35 128
Msa0826820 AT3G54890.1 40.465 215 95 8 60 270 48 233 4.20e-35 127
Msa0826820 AT4G10340.1 39.184 245 111 10 42 274 52 270 3.64e-34 125
Msa0826820 AT1G76570.1 31.271 291 157 10 2 275 62 326 4.51e-30 115
Msa0826820 AT3G08940.2 30.935 278 121 6 40 274 35 284 8.15e-28 108
Msa0826820 AT2G05100.2 35.316 269 130 14 1 255 1 239 2.30e-27 108
Msa0826820 AT5G01530.1 30.038 263 113 6 55 274 53 287 1.39e-24 100
Msa0826820 AT1G15820.1 33.735 249 114 13 24 247 24 246 3.26e-24 98.6
Msa0826820 AT3G54890.4 37.079 178 80 7 60 234 48 196 1.28e-21 90.5
Msa0826820 AT2G40100.1 30.400 250 102 8 52 258 51 271 8.85e-17 78.6
Msa0826820 AT3G54890.2 31.532 222 71 9 60 270 48 199 1.61e-16 76.6
Msa0826820 AT5G28450.1 43.137 102 55 2 174 274 71 170 2.06e-16 75.5

Find 118 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGCTTTGCCGAGAATTTC+AGG 0.135748 5_3:-67608229 None:intergenic
GTTAGCTATGTTGGCAATAT+TGG 0.175999 5_3:+67608570 Msa0826820:CDS
TTTGTTCAAGCTCTTGTTAC+AGG 0.246233 5_3:+67608598 Msa0826820:CDS
ATATTTAACCCTCTTGGTTT+TGG 0.269352 5_3:+67608493 Msa0826820:CDS
ATGATCACTTGCAGCCTTCC+TGG 0.272420 5_3:+67608073 Msa0826820:intron
ATGGGAAAACAATACTTCTT+AGG 0.280187 5_3:+67608418 Msa0826820:CDS
CCAGATGATCTAAGAGGTTT+TGG 0.293540 5_3:-67608633 None:intergenic
TCATATCTTCCTGATCCATT+TGG 0.346145 5_3:+67606685 Msa0826820:five_prime_UTR
TTAGCTATGTTGGCAATATT+GGG 0.350553 5_3:+67608571 Msa0826820:CDS
TATATAATACATTCATTTAG+TGG 0.359289 5_3:-67608696 None:intergenic
AAAGCAAAGTCTTTCTTACT+TGG 0.368436 5_3:+67607208 Msa0826820:CDS
AGATCACTCCCATCTTCAAT+AGG 0.372428 5_3:+67606882 Msa0826820:CDS
TTAGGACTTGAGAAAGGTCT+TGG 0.373705 5_3:+67608436 Msa0826820:CDS
TGCAGCCTTCCTGGTGACTT+TGG 0.374400 5_3:+67608082 Msa0826820:intron
TTCTTCAAGAATGTAGTAGA+TGG 0.375775 5_3:-67606744 None:intergenic
AATGAATCCACCGGTTCCTT+CGG 0.378254 5_3:-67608131 None:intergenic
CCAAAACCTCTTAGATCATC+TGG 0.385529 5_3:+67608633 Msa0826820:CDS
TGCATCCACATGGCTACATT+TGG 0.400944 5_3:+67606653 Msa0826820:five_prime_UTR
GAGAAAGGTCTTGGAGGTTC+CGG 0.403381 5_3:+67608445 Msa0826820:CDS
GGACCTATATTTAACCCTCT+TGG 0.406735 5_3:+67608487 Msa0826820:CDS
ATGAATCCACCGGTTCCTTC+GGG 0.407084 5_3:-67608130 None:intergenic
GAATGGAAGGTTAGCTATGT+TGG 0.408440 5_3:+67608561 Msa0826820:CDS
CAACGGCCGCTTTGCAATGT+TGG 0.410538 5_3:+67608186 Msa0826820:CDS
CAAGCTCTTGTTACAGGAGT+TGG 0.415706 5_3:+67608604 Msa0826820:CDS
AACGGCCGCTTTGCAATGTT+GGG 0.418765 5_3:+67608187 Msa0826820:CDS
CTGCAGGAACATACAACTAC+TGG 0.419959 5_3:+67608305 Msa0826820:CDS
TGTGATGGAAATGGCATTCA+TGG 0.422186 5_3:+67608348 Msa0826820:CDS
AGCCAGGATTGTACCAATCT+TGG 0.425513 5_3:-67608393 None:intergenic
ATACACGTAATATGATGAAT+TGG 0.425683 5_3:-67606549 None:intergenic
GTAATATGATGAATTGGATA+AGG 0.430261 5_3:-67606543 None:intergenic
AACTATAAGCCAAATGGATC+AGG 0.432629 5_3:-67606694 None:intergenic
CTAGAAGACCCTATTGAAGA+TGG 0.437182 5_3:-67606891 None:intergenic
CACACTCTTTGTGATGGAAA+TGG 0.442080 5_3:+67608339 Msa0826820:CDS
GTCAAGATGTTGTTGTTGAC+AGG 0.444048 5_3:-67608661 None:intergenic
CAAAGTCTTTCTTACTTGGA+TGG 0.445116 5_3:+67607212 Msa0826820:CDS
TCAATAGGGTCTTCTAGAAA+AGG 0.446420 5_3:+67606897 Msa0826820:CDS
AAACCAAGAGGGTTAAATAT+AGG 0.452200 5_3:-67608490 None:intergenic
TTTGCAATGTTGGGAGCAGC+CGG 0.456975 5_3:+67608196 Msa0826820:CDS
TAATATGATGAATTGGATAA+GGG 0.458916 5_3:-67606542 None:intergenic
TGAAGTAACTATAAGCCAAA+TGG 0.461090 5_3:-67606700 None:intergenic
GTCTTTCTTACTTGGATGGA+AGG 0.462256 5_3:+67607216 Msa0826820:CDS
AAGACAAGAGAGTGAAGAAA+AGG 0.465124 5_3:-67608799 None:intergenic
AACACAGAAGATTCCAAGAT+TGG 0.467039 5_3:+67608380 Msa0826820:CDS
TCGCCATATGCTAACCATCT+TGG 0.478129 5_3:-67608157 None:intergenic
AAGCTGAAGGAAGTTAAGAA+TGG 0.479661 5_3:+67608544 Msa0826820:CDS
AGTGAGAGATAGACCTTGGC+AGG 0.481039 5_3:-67606951 None:intergenic
CTTTCAGATCCCGAAGGAAC+CGG 0.485171 5_3:+67608121 Msa0826820:CDS
GCTATAGCACCTGAAATTCT+CGG 0.489754 5_3:+67608220 Msa0826820:CDS
AGCTTCTTCTCCTCCTGCCA+AGG 0.490862 5_3:+67606938 Msa0826820:CDS
TGTACGTGTCATTTCTTACA+AGG 0.494586 5_3:-67608854 None:intergenic
TCTCTGTAGCATTATTATTG+TGG 0.499952 5_3:-67609050 None:intergenic
CCTGAAATTCTCGGCAAAGC+AGG 0.501852 5_3:+67608229 Msa0826820:CDS
TTCCCTCCTTTGTTACCTCT+AGG 0.504632 5_3:-67606780 None:intergenic
CATTTAGTGGAACTTGAGAC+TGG 0.504734 5_3:-67608683 None:intergenic
GCACTCCCTTGGTTCCAAAC+CGG 0.505888 5_3:+67608271 Msa0826820:CDS
TTGGTACAATCCTGGCTCCA+TGG 0.508162 5_3:+67608399 Msa0826820:CDS
GATCACTCCCATCTTCAATA+GGG 0.512694 5_3:+67606883 Msa0826820:CDS
GAGCCAAGATGGTTAGCATA+TGG 0.514212 5_3:+67608154 Msa0826820:CDS
GAGGCTGTGAGACAGAGTCT+TGG 0.514771 5_3:+67606855 Msa0826820:CDS
GGACTTGAGAAAGGTCTTGG+AGG 0.517064 5_3:+67608439 Msa0826820:CDS
AAATAAAACTTGTAATAAGA+AGG 0.518508 5_3:+67609017 Msa0826820:three_prime_UTR
TAAAGATGAGAAATCATTGA+AGG 0.521323 5_3:+67608516 Msa0826820:CDS
GTGGATTCATTGAGCCAAGA+TGG 0.521507 5_3:+67608143 Msa0826820:CDS
AAAAGGTTCCTTTGTTGTCA+AGG 0.526175 5_3:+67606914 Msa0826820:CDS
TGAAGGAAGTTAAGAATGGA+AGG 0.527760 5_3:+67608548 Msa0826820:CDS
ACAAGAGAGTGAAGAAAAGG+TGG 0.530505 5_3:-67608796 None:intergenic
TCCGGCGAACCAGCTTATCC+TGG 0.533751 5_3:+67608463 Msa0826820:CDS
CAGTAGTTGTATGTTCCTGC+AGG 0.533860 5_3:-67608304 None:intergenic
TTCCAAGATTGGTACAATCC+TGG 0.536164 5_3:+67608391 Msa0826820:CDS
ATAGGTCCACCAGGATAAGC+TGG 0.537101 5_3:-67608472 None:intergenic
AATTTCAGGTGCTATAGCTC+CGG 0.537587 5_3:-67608215 None:intergenic
CAAGGGAGTGCTGTTTCCGC+GGG 0.537601 5_3:-67608259 None:intergenic
ATCACGCCGGTTTGGAACCA+AGG 0.543476 5_3:-67608277 None:intergenic
TAGAAGACCCTATTGAAGAT+GGG 0.548284 5_3:-67606890 None:intergenic
CCAAGGGAGTGCTGTTTCCG+CGG 0.549385 5_3:-67608260 None:intergenic
TACTTCTTAGGACTTGAGAA+AGG 0.549404 5_3:+67608430 Msa0826820:CDS
TTCCTAGAGGTAACAAAGGA+GGG 0.554204 5_3:+67606778 Msa0826820:five_prime_UTR
ACCAGGATAAGCTGGTTCGC+CGG 0.556663 5_3:-67608464 None:intergenic
TGTGAGCATGTTTCATGATG+TGG 0.565203 5_3:+67608831 Msa0826820:three_prime_UTR
ATTACGTGTATAAACATGGT+TGG 0.565397 5_3:+67606561 Msa0826820:five_prime_UTR
GACTTTGGATTTGACCCTCT+AGG 0.569949 5_3:+67608097 Msa0826820:CDS
TAGTTGTATGTTCCTGCAGG+TGG 0.570487 5_3:-67608301 None:intergenic
CAACTACACACTCTTTGTGA+TGG 0.574918 5_3:+67608333 Msa0826820:CDS
ACCGGCGTGATTCCACCTGC+AGG 0.575662 5_3:+67608289 Msa0826820:CDS
TGGTACAATCCTGGCTCCAT+GGG 0.575675 5_3:+67608400 Msa0826820:CDS
GTGATGGAAATGGCATTCAT+GGG 0.581162 5_3:+67608349 Msa0826820:CDS
ATTGAAGGAGTTAAAGCTGA+AGG 0.587176 5_3:+67608531 Msa0826820:CDS
CATCTTTACCAAAACCAAGA+GGG 0.593825 5_3:-67608501 None:intergenic
TCATATTACGTGTATAAACA+TGG 0.603916 5_3:+67606557 Msa0826820:five_prime_UTR
CTTGTTCCTAGAGGTAACAA+AGG 0.604243 5_3:+67606774 Msa0826820:five_prime_UTR
GAGAGATAGACCTTGGCAGG+AGG 0.606963 5_3:-67606948 None:intergenic
GGTCAGCCAGATGATCTAAG+AGG 0.611676 5_3:-67608639 None:intergenic
ATGGAGCAAATAGACAATTG+TGG 0.612091 5_3:+67607177 Msa0826820:CDS
ACATCATGAAACATGCTCAC+AGG 0.612680 5_3:-67608829 None:intergenic
TCTTGGCTCAATGAATCCAC+CGG 0.614464 5_3:-67608140 None:intergenic
GTTCCTAGAGGTAACAAAGG+AGG 0.614577 5_3:+67606777 Msa0826820:five_prime_UTR
TGCAGGAACATACAACTACT+GGG 0.614884 5_3:+67608306 Msa0826820:CDS
AGAAGCTGCCTTGACAACAA+AGG 0.615553 5_3:-67606922 None:intergenic
CGGGATCTGAAAGTCCTAGA+GGG 0.617233 5_3:-67608111 None:intergenic
GGGTTAAATATAGGTCCACC+AGG 0.621905 5_3:-67608481 None:intergenic
TCACGCCGGTTTGGAACCAA+GGG 0.621949 5_3:-67608276 None:intergenic
GGGTCAAATCCAAAGTCACC+AGG 0.625690 5_3:-67608091 None:intergenic
TTCTTGTGGTAGATATTGCA+TGG 0.626511 5_3:-67606621 None:intergenic
ACGTCCACGTTGCATCCACA+TGG 0.626539 5_3:+67606643 Msa0826820:five_prime_UTR
ATGCAACGTGGACGTTCTTG+TGG 0.631074 5_3:-67606635 None:intergenic
CAAAGCAGGTTTAATTCCCG+CGG 0.641267 5_3:+67608243 Msa0826820:CDS
TCAAGATGTTGTTGTTGACA+GGG 0.645710 5_3:-67608660 None:intergenic
TCAGATCCCGAAGGAACCGG+TGG 0.646981 5_3:+67608124 Msa0826820:CDS
TCCTGCAGGTGGAATCACGC+CGG 0.649684 5_3:-67608290 None:intergenic
CAAATCCAAAGTCACCAGGA+AGG 0.653377 5_3:-67608087 None:intergenic
CTAGGACTTTCAGATCCCGA+AGG 0.660487 5_3:+67608115 Msa0826820:CDS
TCGGGATCTGAAAGTCCTAG+AGG 0.663238 5_3:-67608112 None:intergenic
TCATCTTTACCAAAACCAAG+AGG 0.664345 5_3:-67608502 None:intergenic
GCATATGGCGAGATAATCAA+CGG 0.667486 5_3:+67608169 Msa0826820:CDS
TAACAAAGGAGGGAAGAACA+TGG 0.683240 5_3:+67606788 Msa0826820:exon
CTCTGCCAAATGTAGCCATG+TGG 0.685191 5_3:-67606658 None:intergenic
CTGCTCCCAACATTGCAAAG+CGG 0.723368 5_3:-67608192 None:intergenic
GTAGCCATGTGGATGCAACG+TGG 0.793429 5_3:-67606647 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTCTATATTAATTG+TGG + chr5_3:67607665-67607684 Msa0826820:intron 10.0%
!! TATATAATACATTCATTTAG+TGG - chr5_3:67608699-67608718 None:intergenic 15.0%
!! AAATAAAACTTGTAATAAGA+AGG + chr5_3:67609017-67609036 Msa0826820:three_prime_UTR 15.0%
!! TAATATGATGAATTGGATAA+GGG - chr5_3:67606545-67606564 None:intergenic 20.0%
!!! ATAACTTTCTTTTCTCTTTT+TGG + chr5_3:67607143-67607162 Msa0826820:intron 20.0%
!! TTGAACAAAAAACTATTGTA+AGG + chr5_3:67607254-67607273 Msa0826820:intron 20.0%
!!! AGTATGAAATTGACTTTATT+CGG + chr5_3:67608046-67608065 Msa0826820:intron 20.0%
! GTAATATGATGAATTGGATA+AGG - chr5_3:67606546-67606565 None:intergenic 25.0%
! ATACACGTAATATGATGAAT+TGG - chr5_3:67606552-67606571 None:intergenic 25.0%
! TCATATTACGTGTATAAACA+TGG + chr5_3:67606557-67606576 Msa0826820:five_prime_UTR 25.0%
!!! AGTTTTTTGTTCAACTACTA+AGG - chr5_3:67607248-67607267 None:intergenic 25.0%
! AACAAAAAACTATTGTAAGG+TGG + chr5_3:67607257-67607276 Msa0826820:intron 25.0%
! CTTGATTAAACTGTTTACTT+AGG - chr5_3:67607319-67607338 None:intergenic 25.0%
! ATCTACATGTTGTAATGTTA+AGG - chr5_3:67607603-67607622 None:intergenic 25.0%
! TTAATTGTGGTAACTTTGTA+TGG + chr5_3:67607678-67607697 Msa0826820:intron 25.0%
! TGTCTTTAGATTATTTCGAT+AGG - chr5_3:67607863-67607882 None:intergenic 25.0%
! CAATCACTTGTCTAAATATT+TGG - chr5_3:67607924-67607943 None:intergenic 25.0%
! ATGTTGTATACTTGTGTTAT+GGG - chr5_3:67607985-67608004 None:intergenic 25.0%
! TAAAGATGAGAAATCATTGA+AGG + chr5_3:67608516-67608535 Msa0826820:CDS 25.0%
ATTACGTGTATAAACATGGT+TGG + chr5_3:67606561-67606580 Msa0826820:five_prime_UTR 30.0%
TGAAGTAACTATAAGCCAAA+TGG - chr5_3:67606703-67606722 None:intergenic 30.0%
TTCTTCAAGAATGTAGTAGA+TGG - chr5_3:67606747-67606766 None:intergenic 30.0%
! AAGAAAAAACTTGTTCCTAG+AGG + chr5_3:67606765-67606784 Msa0826820:five_prime_UTR 30.0%
ATATTACAACTGTCTCATGT+GGG + chr5_3:67607030-67607049 Msa0826820:intron 30.0%
TTGTATATTTGTCAGTTCAG+TGG - chr5_3:67607096-67607115 None:intergenic 30.0%
AAAGCAAAGTCTTTCTTACT+TGG + chr5_3:67607208-67607227 Msa0826820:CDS 30.0%
ATGTAGATATGCAACTTTGT+TGG + chr5_3:67607615-67607634 Msa0826820:intron 30.0%
AAAATCTCAAAAGCCTTCTA+GGG - chr5_3:67607726-67607745 None:intergenic 30.0%
AAAAATCTCAAAAGCCTTCT+AGG - chr5_3:67607727-67607746 None:intergenic 30.0%
ATTGTGTTAGAACATAAGCT+TGG + chr5_3:67607940-67607959 Msa0826820:intron 30.0%
CATGTTGTATACTTGTGTTA+TGG - chr5_3:67607986-67608005 None:intergenic 30.0%
!!! TAAGAAGTATTGTTTTCCCA+TGG - chr5_3:67608419-67608438 None:intergenic 30.0%
ATGGGAAAACAATACTTCTT+AGG + chr5_3:67608418-67608437 Msa0826820:CDS 30.0%
AAACCAAGAGGGTTAAATAT+AGG - chr5_3:67608493-67608512 None:intergenic 30.0%
! ATATTTAACCCTCTTGGTTT+TGG + chr5_3:67608493-67608512 Msa0826820:CDS 30.0%
TTAGCTATGTTGGCAATATT+GGG + chr5_3:67608571-67608590 Msa0826820:CDS 30.0%
! TTTCTTTTCTTTGCTCTTGT+TGG + chr5_3:67608737-67608756 Msa0826820:three_prime_UTR 30.0%
!!! AAAAGGTGGTTGAGTTTTTT+TGG - chr5_3:67608785-67608804 None:intergenic 30.0%
TCTCTGTAGCATTATTATTG+TGG - chr5_3:67609053-67609072 None:intergenic 30.0%
AGCAAAAACGTAATAGCAGT+GGG + chr5_3:67606505-67606524 Msa0826820:five_prime_UTR 35.0%
TTCTTGTGGTAGATATTGCA+TGG - chr5_3:67606624-67606643 None:intergenic 35.0%
TCATATCTTCCTGATCCATT+TGG + chr5_3:67606685-67606704 Msa0826820:five_prime_UTR 35.0%
AACTATAAGCCAAATGGATC+AGG - chr5_3:67606697-67606716 None:intergenic 35.0%
TAGAAGACCCTATTGAAGAT+GGG - chr5_3:67606893-67606912 None:intergenic 35.0%
! TCAATAGGGTCTTCTAGAAA+AGG + chr5_3:67606897-67606916 Msa0826820:CDS 35.0%
! AAAAGGTTCCTTTGTTGTCA+AGG + chr5_3:67606914-67606933 Msa0826820:CDS 35.0%
CACATGAGACAGTTGTAATA+TGG - chr5_3:67607031-67607050 None:intergenic 35.0%
CATATTACAACTGTCTCATG+TGG + chr5_3:67607029-67607048 Msa0826820:intron 35.0%
!!! CTTTTTGGTGTTCAGCAAAA+TGG + chr5_3:67607158-67607177 Msa0826820:intron 35.0%
ATGGAGCAAATAGACAATTG+TGG + chr5_3:67607177-67607196 Msa0826820:CDS 35.0%
CAAAGTCTTTCTTACTTGGA+TGG + chr5_3:67607212-67607231 Msa0826820:CDS 35.0%
TGTTTACTTAGGCATACTCT+AGG - chr5_3:67607308-67607327 None:intergenic 35.0%
AGAACATAAGCTTGGATACT+TGG + chr5_3:67607948-67607967 Msa0826820:intron 35.0%
AACACAGAAGATTCCAAGAT+TGG + chr5_3:67608380-67608399 Msa0826820:CDS 35.0%
TACTTCTTAGGACTTGAGAA+AGG + chr5_3:67608430-67608449 Msa0826820:CDS 35.0%
CATCTTTACCAAAACCAAGA+GGG - chr5_3:67608504-67608523 None:intergenic 35.0%
TCATCTTTACCAAAACCAAG+AGG - chr5_3:67608505-67608524 None:intergenic 35.0%
ATTGAAGGAGTTAAAGCTGA+AGG + chr5_3:67608531-67608550 Msa0826820:CDS 35.0%
AAGCTGAAGGAAGTTAAGAA+TGG + chr5_3:67608544-67608563 Msa0826820:CDS 35.0%
TGAAGGAAGTTAAGAATGGA+AGG + chr5_3:67608548-67608567 Msa0826820:CDS 35.0%
GTTAGCTATGTTGGCAATAT+TGG + chr5_3:67608570-67608589 Msa0826820:CDS 35.0%
TTTGTTCAAGCTCTTGTTAC+AGG + chr5_3:67608598-67608617 Msa0826820:CDS 35.0%
!! TGATCTAAGAGGTTTTGGTA+TGG - chr5_3:67608631-67608650 None:intergenic 35.0%
! TCAAGATGTTGTTGTTGACA+GGG - chr5_3:67608663-67608682 None:intergenic 35.0%
AAGACAAGAGAGTGAAGAAA+AGG - chr5_3:67608802-67608821 None:intergenic 35.0%
!! TGTACGTGTCATTTCTTACA+AGG - chr5_3:67608857-67608876 None:intergenic 35.0%
CAGCAAAAACGTAATAGCAG+TGG + chr5_3:67606504-67606523 Msa0826820:five_prime_UTR 40.0%
CTTGTTCCTAGAGGTAACAA+AGG + chr5_3:67606774-67606793 Msa0826820:five_prime_UTR 40.0%
TTCCTAGAGGTAACAAAGGA+GGG + chr5_3:67606778-67606797 Msa0826820:five_prime_UTR 40.0%
TAACAAAGGAGGGAAGAACA+TGG + chr5_3:67606788-67606807 Msa0826820:exon 40.0%
!! ATCTCTTACTTTTGCAGCTG+AGG + chr5_3:67606836-67606855 Msa0826820:CDS 40.0%
AGATCACTCCCATCTTCAAT+AGG + chr5_3:67606882-67606901 Msa0826820:CDS 40.0%
GATCACTCCCATCTTCAATA+GGG + chr5_3:67606883-67606902 Msa0826820:CDS 40.0%
CTAGAAGACCCTATTGAAGA+TGG - chr5_3:67606894-67606913 None:intergenic 40.0%
TTTGAGTGAGAGATAGACCT+TGG - chr5_3:67606958-67606977 None:intergenic 40.0%
GTCTTTCTTACTTGGATGGA+AGG + chr5_3:67607216-67607235 Msa0826820:CDS 40.0%
TTATGCAAGTGCATGTGTTG+AGG + chr5_3:67607544-67607563 Msa0826820:intron 40.0%
! TGTGAGTCTAAAACCCTAGA+AGG + chr5_3:67607710-67607729 Msa0826820:intron 40.0%
GCATATGGCGAGATAATCAA+CGG + chr5_3:67608169-67608188 Msa0826820:CDS 40.0%
! AATTTCAGGTGCTATAGCTC+CGG - chr5_3:67608218-67608237 None:intergenic 40.0%
GCTATAGCACCTGAAATTCT+CGG + chr5_3:67608220-67608239 Msa0826820:CDS 40.0%
TGCAGGAACATACAACTACT+GGG + chr5_3:67608306-67608325 Msa0826820:CDS 40.0%
CAACTACACACTCTTTGTGA+TGG + chr5_3:67608333-67608352 Msa0826820:CDS 40.0%
! CACACTCTTTGTGATGGAAA+TGG + chr5_3:67608339-67608358 Msa0826820:CDS 40.0%
! TGTGATGGAAATGGCATTCA+TGG + chr5_3:67608348-67608367 Msa0826820:CDS 40.0%
! GTGATGGAAATGGCATTCAT+GGG + chr5_3:67608349-67608368 Msa0826820:CDS 40.0%
TTCCAAGATTGGTACAATCC+TGG + chr5_3:67608391-67608410 Msa0826820:CDS 40.0%
TTAGGACTTGAGAAAGGTCT+TGG + chr5_3:67608436-67608455 Msa0826820:CDS 40.0%
GGACCTATATTTAACCCTCT+TGG + chr5_3:67608487-67608506 Msa0826820:CDS 40.0%
! GAATGGAAGGTTAGCTATGT+TGG + chr5_3:67608561-67608580 Msa0826820:CDS 40.0%
! CCAGATGATCTAAGAGGTTT+TGG - chr5_3:67608636-67608655 None:intergenic 40.0%
CCAAAACCTCTTAGATCATC+TGG + chr5_3:67608633-67608652 Msa0826820:CDS 40.0%
! GTCAAGATGTTGTTGTTGAC+AGG - chr5_3:67608664-67608683 None:intergenic 40.0%
CATTTAGTGGAACTTGAGAC+TGG - chr5_3:67608686-67608705 None:intergenic 40.0%
ACAAGAGAGTGAAGAAAAGG+TGG - chr5_3:67608799-67608818 None:intergenic 40.0%
ACATCATGAAACATGCTCAC+AGG - chr5_3:67608832-67608851 None:intergenic 40.0%
TGTGAGCATGTTTCATGATG+TGG + chr5_3:67608831-67608850 Msa0826820:three_prime_UTR 40.0%
TGCATCCACATGGCTACATT+TGG + chr5_3:67606653-67606672 Msa0826820:five_prime_UTR 45.0%
GTTCCTAGAGGTAACAAAGG+AGG + chr5_3:67606777-67606796 Msa0826820:five_prime_UTR 45.0%
TTCCCTCCTTTGTTACCTCT+AGG - chr5_3:67606783-67606802 None:intergenic 45.0%
AGAAGCTGCCTTGACAACAA+AGG - chr5_3:67606925-67606944 None:intergenic 45.0%
CAAATCCAAAGTCACCAGGA+AGG - chr5_3:67608090-67608109 None:intergenic 45.0%
GACTTTGGATTTGACCCTCT+AGG + chr5_3:67608097-67608116 Msa0826820:CDS 45.0%
!! AATGAATCCACCGGTTCCTT+CGG - chr5_3:67608134-67608153 None:intergenic 45.0%
TCTTGGCTCAATGAATCCAC+CGG - chr5_3:67608143-67608162 None:intergenic 45.0%
GTGGATTCATTGAGCCAAGA+TGG + chr5_3:67608143-67608162 Msa0826820:CDS 45.0%
GAGCCAAGATGGTTAGCATA+TGG + chr5_3:67608154-67608173 Msa0826820:CDS 45.0%
TCGCCATATGCTAACCATCT+TGG - chr5_3:67608160-67608179 None:intergenic 45.0%
CAAAGCAGGTTTAATTCCCG+CGG + chr5_3:67608243-67608262 Msa0826820:CDS 45.0%
TAGTTGTATGTTCCTGCAGG+TGG - chr5_3:67608304-67608323 None:intergenic 45.0%
CAGTAGTTGTATGTTCCTGC+AGG - chr5_3:67608307-67608326 None:intergenic 45.0%
CTGCAGGAACATACAACTAC+TGG + chr5_3:67608305-67608324 Msa0826820:CDS 45.0%
AGCCAGGATTGTACCAATCT+TGG - chr5_3:67608396-67608415 None:intergenic 45.0%
! TATTGTTTTCCCATGGAGCC+AGG - chr5_3:67608412-67608431 None:intergenic 45.0%
GGGTTAAATATAGGTCCACC+AGG - chr5_3:67608484-67608503 None:intergenic 45.0%
CAAGCTCTTGTTACAGGAGT+TGG + chr5_3:67608604-67608623 Msa0826820:CDS 45.0%
!! TAATATAGAAAATAAAAAAT+TGG - chr5_3:67607661-67607680 None:intergenic 5.0%
ATGCAACGTGGACGTTCTTG+TGG - chr5_3:67606638-67606657 None:intergenic 50.0%
CTCTGCCAAATGTAGCCATG+TGG - chr5_3:67606661-67606680 None:intergenic 50.0%
AGTGAGAGATAGACCTTGGC+AGG - chr5_3:67606954-67606973 None:intergenic 50.0%
ATGATCACTTGCAGCCTTCC+TGG + chr5_3:67608073-67608092 Msa0826820:intron 50.0%
GGGTCAAATCCAAAGTCACC+AGG - chr5_3:67608094-67608113 None:intergenic 50.0%
CGGGATCTGAAAGTCCTAGA+GGG - chr5_3:67608114-67608133 None:intergenic 50.0%
TCGGGATCTGAAAGTCCTAG+AGG - chr5_3:67608115-67608134 None:intergenic 50.0%
CTAGGACTTTCAGATCCCGA+AGG + chr5_3:67608115-67608134 Msa0826820:CDS 50.0%
CTTTCAGATCCCGAAGGAAC+CGG + chr5_3:67608121-67608140 Msa0826820:CDS 50.0%
!! ATGAATCCACCGGTTCCTTC+GGG - chr5_3:67608133-67608152 None:intergenic 50.0%
! AACGGCCGCTTTGCAATGTT+GGG + chr5_3:67608187-67608206 Msa0826820:CDS 50.0%
CTGCTCCCAACATTGCAAAG+CGG - chr5_3:67608195-67608214 None:intergenic 50.0%
TTTGCAATGTTGGGAGCAGC+CGG + chr5_3:67608196-67608215 Msa0826820:CDS 50.0%
CCTGCTTTGCCGAGAATTTC+AGG - chr5_3:67608232-67608251 None:intergenic 50.0%
CCTGAAATTCTCGGCAAAGC+AGG + chr5_3:67608229-67608248 Msa0826820:CDS 50.0%
TTGGTACAATCCTGGCTCCA+TGG + chr5_3:67608399-67608418 Msa0826820:CDS 50.0%
TGGTACAATCCTGGCTCCAT+GGG + chr5_3:67608400-67608419 Msa0826820:CDS 50.0%
GGACTTGAGAAAGGTCTTGG+AGG + chr5_3:67608439-67608458 Msa0826820:CDS 50.0%
GAGAAAGGTCTTGGAGGTTC+CGG + chr5_3:67608445-67608464 Msa0826820:CDS 50.0%
ATAGGTCCACCAGGATAAGC+TGG - chr5_3:67608475-67608494 None:intergenic 50.0%
GGTCAGCCAGATGATCTAAG+AGG - chr5_3:67608642-67608661 None:intergenic 50.0%
ACGTCCACGTTGCATCCACA+TGG + chr5_3:67606643-67606662 Msa0826820:five_prime_UTR 55.0%
GTAGCCATGTGGATGCAACG+TGG - chr5_3:67606650-67606669 None:intergenic 55.0%
GAGGCTGTGAGACAGAGTCT+TGG + chr5_3:67606855-67606874 Msa0826820:CDS 55.0%
AGCTTCTTCTCCTCCTGCCA+AGG + chr5_3:67606938-67606957 Msa0826820:CDS 55.0%
GAGAGATAGACCTTGGCAGG+AGG - chr5_3:67606951-67606970 None:intergenic 55.0%
TGCAGCCTTCCTGGTGACTT+TGG + chr5_3:67608082-67608101 Msa0826820:intron 55.0%
! CAACGGCCGCTTTGCAATGT+TGG + chr5_3:67608186-67608205 Msa0826820:CDS 55.0%
GCACTCCCTTGGTTCCAAAC+CGG + chr5_3:67608271-67608290 Msa0826820:CDS 55.0%
!! TCACGCCGGTTTGGAACCAA+GGG - chr5_3:67608279-67608298 None:intergenic 55.0%
!! ATCACGCCGGTTTGGAACCA+AGG - chr5_3:67608280-67608299 None:intergenic 55.0%
!! ACCAGGATAAGCTGGTTCGC+CGG - chr5_3:67608467-67608486 None:intergenic 55.0%
TCAGATCCCGAAGGAACCGG+TGG + chr5_3:67608124-67608143 Msa0826820:CDS 60.0%
!! CAAGGGAGTGCTGTTTCCGC+GGG - chr5_3:67608262-67608281 None:intergenic 60.0%
!! CCAAGGGAGTGCTGTTTCCG+CGG - chr5_3:67608263-67608282 None:intergenic 60.0%
! CAGGTGGAATCACGCCGGTT+TGG - chr5_3:67608288-67608307 None:intergenic 60.0%
TCCTGCAGGTGGAATCACGC+CGG - chr5_3:67608293-67608312 None:intergenic 60.0%
TCCGGCGAACCAGCTTATCC+TGG + chr5_3:67608463-67608482 Msa0826820:CDS 60.0%
GGCGAACCAGCTTATCCTGG+TGG + chr5_3:67608466-67608485 Msa0826820:CDS 60.0%
CCGCGGAAACAGCACTCCCT+TGG + chr5_3:67608260-67608279 Msa0826820:CDS 65.0%
ACCGGCGTGATTCCACCTGC+AGG + chr5_3:67608289-67608308 Msa0826820:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 67606489 67609080 67606489 ID=Msa0826820;Name=Msa0826820
chr5_3 mRNA 67606489 67609080 67606489 ID=Msa0826820-mRNA-1;Parent=Msa0826820;Name=Msa0826820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=318|1|1|1|1|1|3|378|277
chr5_3 exon 67606489 67606959 67606489 ID=Msa0826820-mRNA-1:exon:21158;Parent=Msa0826820-mRNA-1
chr5_3 exon 67607173 67607237 67607173 ID=Msa0826820-mRNA-1:exon:21159;Parent=Msa0826820-mRNA-1
chr5_3 exon 67608087 67609080 67608087 ID=Msa0826820-mRNA-1:exon:21160;Parent=Msa0826820-mRNA-1
chr5_3 five_prime_UTR 67606489 67606806 67606489 ID=Msa0826820-mRNA-1:five_prime_utr;Parent=Msa0826820-mRNA-1
chr5_3 CDS 67606807 67606959 67606807 ID=Msa0826820-mRNA-1:cds;Parent=Msa0826820-mRNA-1
chr5_3 CDS 67607173 67607237 67607173 ID=Msa0826820-mRNA-1:cds;Parent=Msa0826820-mRNA-1
chr5_3 CDS 67608087 67608702 67608087 ID=Msa0826820-mRNA-1:cds;Parent=Msa0826820-mRNA-1
chr5_3 three_prime_UTR 67608703 67609080 67608703 ID=Msa0826820-mRNA-1:three_prime_utr;Parent=Msa0826820-mRNA-1
Gene Sequence

>Msa0826820

ATGGCTACACAAGCTCTAGTGTCGTCATCATCTCTTACTTTTGCAGCTGAGGCTGTGAGACAGAGTCTTGGAGCAAGATCACTCCCATCTTCAATAGGGTCTTCTAGAAAAGGTTCCTTTGTTGTCAAGGCAGCTTCTTCTCCTCCTGCCAAGCAAAATGGAGCAAATAGACAATTGTGGTTTGCATCAAAGCAAAGTCTTTCTTACTTGGATGGAAGCCTTCCTGGTGACTTTGGATTTGACCCTCTAGGACTTTCAGATCCCGAAGGAACCGGTGGATTCATTGAGCCAAGATGGTTAGCATATGGCGAGATAATCAACGGCCGCTTTGCAATGTTGGGAGCAGCCGGAGCTATAGCACCTGAAATTCTCGGCAAAGCAGGTTTAATTCCCGCGGAAACAGCACTCCCTTGGTTCCAAACCGGCGTGATTCCACCTGCAGGAACATACAACTACTGGGCAGACAACTACACACTCTTTGTGATGGAAATGGCATTCATGGGATTTGCAGAACACAGAAGATTCCAAGATTGGTACAATCCTGGCTCCATGGGAAAACAATACTTCTTAGGACTTGAGAAAGGTCTTGGAGGTTCCGGCGAACCAGCTTATCCTGGTGGACCTATATTTAACCCTCTTGGTTTTGGTAAAGATGAGAAATCATTGAAGGAGTTAAAGCTGAAGGAAGTTAAGAATGGAAGGTTAGCTATGTTGGCAATATTGGGTTACTTTGTTCAAGCTCTTGTTACAGGAGTTGGACCATACCAAAACCTCTTAGATCATCTGGCTGACCCTGTCAACAACAACATCTTGACCAGTCTCAAGTTCCACTAA
Protein sequence

>Msa0826820

MATQALVSSSSLTFAAEAVRQSLGARSLPSSIGSSRKGSFVVKAASSPPAKQNGANRQLWFASKQSLSYLDGSLPGDFGFDPLGLSDPEGTGGFIEPRWLAYGEIINGRFAMLGAAGAIAPEILGKAGLIPAETALPWFQTGVIPPAGTYNYWADNYTLFVMEMAFMGFAEHRRFQDWYNPGSMGKQYFLGLEKGLGGSGEPAYPGGPIFNPLGFGKDEKSLKELKLKEVKNGRLAMLAILGYFVQALVTGVGPYQNLLDHLADPVNNNILTSLKFH*