AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1227350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227350 MtrunA17_Chr1g0155981 100.000 206 0 0 1 206 1 206 1.12e-150 416
Msa1227350 MtrunA17_Chr7g0237121 94.660 206 11 0 1 206 1 206 1.49e-143 398
Msa1227350 MtrunA17_Chr5g0437981 91.262 206 18 0 1 206 1 206 3.20e-138 384
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227350 AT5G48760.1 85.922 206 29 0 1 206 1 206 1.91e-131 368
Msa1227350 AT5G48760.2 85.922 206 29 0 1 206 1 206 1.91e-131 368
Msa1227350 AT3G07110.1 84.951 206 31 0 1 206 1 206 6.59e-130 364
Msa1227350 AT4G13170.1 84.951 206 31 0 1 206 1 206 9.06e-130 363
Msa1227350 AT3G07110.2 84.541 207 31 1 1 206 1 207 2.75e-128 360
Msa1227350 AT3G24830.1 83.495 206 34 0 1 206 1 206 5.30e-128 359

Find 96 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGGTCCTATTCACTTTA+GGG 0.192347 8_2:+2459000 Msa1227350:CDS
CTCATGGTCCTATTCACTTT+AGG 0.198145 8_2:+2458999 Msa1227350:CDS
ATCTCAAATGCAGGATATTT+AGG 0.278445 8_2:-2462513 None:intergenic
GGGCTCCCTCTAAGATCTTT+TGG 0.279612 8_2:+2459020 Msa1227350:CDS
TGAGATGAATATAGACTAAC+TGG 0.300848 8_2:+2462530 Msa1227350:three_prime_UTR
CATTATTATAAGGTTTAGTT+TGG 0.304616 8_2:+2462245 Msa1227350:three_prime_UTR
GCTTGTTATTCTATGTTTGT+TGG 0.318515 8_2:+2462309 Msa1227350:three_prime_UTR
TATTTGTTAACATTATTATA+AGG 0.320139 8_2:+2462235 Msa1227350:three_prime_UTR
TTCCAATAGTGTTGCCCCTT+TGG 0.322660 8_2:+2462404 Msa1227350:three_prime_UTR
ACTATGAAGCTTATAGTTGT+TGG 0.329462 8_2:+2462467 Msa1227350:three_prime_UTR
CACCAAAGGGGCAACACTAT+TGG 0.342959 8_2:-2462406 None:intergenic
GACGGCCGAGCATGTGGTGG+CGG 0.371739 8_2:-2458832 None:intergenic
GGGAGCAAGGACTTCAAGTT+GGG 0.380650 8_2:-2462196 None:intergenic
TATAGGAGCTGGAGGATAAG+AGG 0.397991 8_2:+2462082 Msa1227350:intron
TGTGAAGAAATTTGTGCTTC+AGG 0.399041 8_2:+2458911 Msa1227350:CDS
AATAACAAGCAAAACTGAAA+TGG 0.403835 8_2:-2462296 None:intergenic
TGAAGTATATGAGATTTCTA+AGG 0.407625 8_2:+2458954 Msa1227350:CDS
CAGGATATTTAGGCACCTAT+TGG 0.413197 8_2:-2462503 None:intergenic
GGCTGCACTCGCGCGATTGA+AGG 0.416813 8_2:+2461721 Msa1227350:CDS
GAGGAAGGAACGCGCAGGTT+TGG 0.417217 8_2:+2462101 Msa1227350:CDS
ATATATGATGTTACACCAAA+GGG 0.421265 8_2:-2462419 None:intergenic
GCGGCGAGGCTGTTCTTCTC+CGG 0.423588 8_2:-2458743 None:intergenic
CGGCGAGGCTGTTCTTCTCC+GGG 0.424749 8_2:-2458742 None:intergenic
GTTCTTCTCCGGGGACAGCT+AGG 0.425336 8_2:-2458732 None:intergenic
TAGGGTGTTGAGACTTCAAA+AGG 0.434162 8_2:+2461904 Msa1227350:intron
ATCCTCTTGATCTTGTCATA+TGG 0.437541 8_2:-2461764 None:intergenic
CGGGAGCAAGGACTTCAAGT+TGG 0.439216 8_2:-2462197 None:intergenic
AATAGTACTCAGTACTTAAC+GGG 0.449959 8_2:-2462216 None:intergenic
AAATAGTACTCAGTACTTAA+CGG 0.452401 8_2:-2462217 None:intergenic
GGATTATTGATGCTGCCAAT+AGG 0.469766 8_2:+2462488 Msa1227350:three_prime_UTR
GAGGGAGCCCTAAAGTGAAT+AGG 0.470476 8_2:-2459007 None:intergenic
GGTTCTTACTTGAGAGCATC+GGG 0.471713 8_2:-2461794 None:intergenic
AGAAGCTGCTCAATAAATTG+AGG 0.480348 8_2:+2462136 Msa1227350:CDS
AACACTATTGGAAAGTAAGT+TGG 0.484076 8_2:-2462394 None:intergenic
AACCTAAAACAGTTCATGTT+GGG 0.486376 8_2:+2458614 Msa1227350:five_prime_UTR
ATATGAGATTTCTAAGGAAG+AGG 0.501956 8_2:+2458960 Msa1227350:CDS
GAGAAGAACAGCCTCGCCGC+CGG 0.505808 8_2:+2458746 Msa1227350:five_prime_UTR
TTCTTCTCCGGGGACAGCTA+GGG 0.509116 8_2:-2458731 None:intergenic
CGCGCGATTGAAGGTCTATG+AGG 0.509761 8_2:+2461730 Msa1227350:CDS
CTTAGCGCATATGCCTGATC+CGG 0.515048 8_2:-2458792 None:intergenic
GAAGCTGCTCAATAAATTGA+GGG 0.519049 8_2:+2462137 Msa1227350:CDS
GACGCCCGCCACCACATGCT+CGG 0.519214 8_2:+2458827 Msa1227350:CDS
ACGGCCGAGCATGTGGTGGC+GGG 0.522437 8_2:-2458831 None:intergenic
TCTATATTCATCTCAAATGC+AGG 0.528432 8_2:-2462522 None:intergenic
AGTGCGCCAAAAGATCTTAG+AGG 0.534021 8_2:-2459026 None:intergenic
TGGTTCTTACTTGAGAGCAT+CGG 0.539745 8_2:-2461795 None:intergenic
GAGAAGCTCCTTTGCGACAA+TGG 0.544115 8_2:-2458861 None:intergenic
TTCTTGTTTATAGGAGCTGG+AGG 0.546787 8_2:+2462074 Msa1227350:intron
GAACCTAAAACAGTTCATGT+TGG 0.549953 8_2:+2458613 Msa1227350:five_prime_UTR
GGACACAAATACTGCTTGCT+CGG 0.550212 8_2:+2461926 Msa1227350:CDS
ATGAATACTAAACCTTCTCA+TGG 0.560213 8_2:+2458983 Msa1227350:CDS
CTCAGTACTTAACGGGAGCA+AGG 0.561409 8_2:-2462209 None:intergenic
GTCGCAAAGGAGCTTCTCAA+TGG 0.561981 8_2:+2458866 Msa1227350:CDS
ATGAAGTTTCCGCGGGCACA+AGG 0.562034 8_2:-2462563 None:intergenic
AGTTGTCATCAGAGGTTGGA+TGG 0.565112 8_2:+2461951 Msa1227350:CDS
GTACTTTCACCTTGTGCCCG+CGG 0.569360 8_2:+2462554 Msa1227350:three_prime_UTR
CATCAAACCCTAGCTGTCCC+CGG 0.571320 8_2:+2458724 Msa1227350:five_prime_UTR
AGGCAAGACGGCCGAGCATG+TGG 0.572354 8_2:-2458838 None:intergenic
TTGATCTTGTCATATGGAGG+AGG 0.574582 8_2:-2461758 None:intergenic
CTCCATATGACAAGATCAAG+AGG 0.577162 8_2:+2461762 Msa1227350:CDS
TCTTGCCTCCATTGTCGCAA+AGG 0.579194 8_2:+2458853 Msa1227350:CDS
CATATATGATGTTACACCAA+AGG 0.581184 8_2:-2462420 None:intergenic
ACAGCTAGGGTTTGATGATG+AGG 0.581750 8_2:-2458718 None:intergenic
TCAGAGGTTGGATGGAACTA+CGG 0.586349 8_2:+2461959 Msa1227350:CDS
GGAGCTGGAGGATAAGAGGA+AGG 0.587458 8_2:+2462086 Msa1227350:intron
GAACTACGGTGGCATCATCA+AGG 0.590785 8_2:+2461973 Msa1227350:CDS
TTAGAAAATGAAGTTTCCGC+GGG 0.591210 8_2:-2462570 None:intergenic
GGACAGTTGTCATCAGAGGT+TGG 0.592395 8_2:+2461947 Msa1227350:CDS
CTCTTGATCTTGTCATATGG+AGG 0.593959 8_2:-2461761 None:intergenic
GCTCGGACAGTTGTCATCAG+AGG 0.597789 8_2:+2461943 Msa1227350:CDS
GGAGCTTCTCAATGGTCAGA+AGG 0.600116 8_2:+2458874 Msa1227350:CDS
GTGCGCCAAAAGATCTTAGA+GGG 0.602717 8_2:-2459025 None:intergenic
AGAATCTCTTTCCGGCGGCG+AGG 0.602849 8_2:-2458757 None:intergenic
CAAGACGGCCGAGCATGTGG+TGG 0.603581 8_2:-2458835 None:intergenic
CTTAGAAAATGAAGTTTCCG+CGG 0.608511 8_2:-2462571 None:intergenic
TTGCGACAATGGAGGCAAGA+CGG 0.613146 8_2:-2458850 None:intergenic
AGGGTGTTGAGACTTCAAAA+GGG 0.620018 8_2:+2461905 Msa1227350:intron
GCGCGATTGAAGGTCTATGA+GGG 0.624683 8_2:+2461731 Msa1227350:CDS
CTAAGCTTAGGGGTTTGTCG+TGG 0.626595 8_2:+2462589 Msa1227350:three_prime_UTR
ATATGACAAGATCAAGAGGA+TGG 0.634497 8_2:+2461766 Msa1227350:CDS
GGAGCAAGGACTTCAAGTTG+GGG 0.640419 8_2:-2462195 None:intergenic
TCTCCACGCTTAGTCTTGTG+TGG 0.642845 8_2:-2461698 None:intergenic
ATACCACACAAGACTAAGCG+TGG 0.643021 8_2:+2461695 Msa1227350:CDS
AAGCTCCTTTGCGACAATGG+AGG 0.645821 8_2:-2458858 None:intergenic
AGCAAGGACTTCAAGTTGGG+GGG 0.650449 8_2:-2462193 None:intergenic
GATAAGAGGAAGGAACGCGC+AGG 0.659068 8_2:+2462096 Msa1227350:CDS
GCTTCTCAATGGTCAGAAGG+TGG 0.662927 8_2:+2458877 Msa1227350:CDS
GGCGAGGCTGTTCTTCTCCG+GGG 0.663395 8_2:-2458741 None:intergenic
ACACAAGACTAAGCGTGGAG+AGG 0.667676 8_2:+2461700 Msa1227350:CDS
AAGTGAATAGGACCATGAGA+AGG 0.669515 8_2:-2458995 None:intergenic
GAGATGAATATAGACTAACT+GGG 0.687169 8_2:+2462531 Msa1227350:three_prime_UTR
GAGCAAGGACTTCAAGTTGG+GGG 0.688692 8_2:-2462194 None:intergenic
GAAGAAATTTGTGCTTCAGG+TGG 0.691821 8_2:+2458914 Msa1227350:CDS
GAGGTTGGATGGAACTACGG+TGG 0.706495 8_2:+2461962 Msa1227350:CDS
TATATGATGTTACACCAAAG+GGG 0.708571 8_2:-2462418 None:intergenic
AGATGAATATAGACTAACTG+GGG 0.722189 8_2:+2462532 Msa1227350:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTATAAATTTATT+AGG + chr8_2:2460594-2460613 Msa1227350:intron 0.0%
!!! ATTTTTGTTTAATTTGATAT+TGG + chr8_2:2459121-2459140 Msa1227350:intron 10.0%
!!! TTTTTGTTTAATTTGATATT+GGG + chr8_2:2459122-2459141 Msa1227350:intron 10.0%
!!! TTTTCTTAATTAAATTTTGT+TGG + chr8_2:2460500-2460519 Msa1227350:intron 10.0%
!!! TTTCTTAATTAAATTTTGTT+GGG + chr8_2:2460501-2460520 Msa1227350:intron 10.0%
!!! TTTTTTTGATTATTTGATAA+TGG + chr8_2:2460537-2460556 Msa1227350:intron 10.0%
!!! TTTTTTGATTATTTGATAAT+GGG + chr8_2:2460538-2460557 Msa1227350:intron 10.0%
!!! GTTTTTTTATATGATTTATA+AGG + chr8_2:2460661-2460680 Msa1227350:intron 10.0%
!!! TATTTGTTAACATTATTATA+AGG + chr8_2:2462235-2462254 Msa1227350:three_prime_UTR 10.0%
!!! TGTATTTGTATTTGTTTTTT+AGG + chr8_2:2459277-2459296 Msa1227350:intron 15.0%
!! TTTGATTATTAGATTGAATT+GGG + chr8_2:2459442-2459461 Msa1227350:intron 15.0%
!!! ATGATTTGTGATTATAAATA+TGG + chr8_2:2459567-2459586 Msa1227350:intron 15.0%
!!! TATGTTATTTCTATAACAAA+AGG - chr8_2:2459843-2459862 None:intergenic 15.0%
!! ATATGATTTATAAGGTTATT+TGG + chr8_2:2460669-2460688 Msa1227350:intron 15.0%
!! CTGTAAATTATACAATAAAA+TGG - chr8_2:2461818-2461837 None:intergenic 15.0%
!!! TTTTATTGTGTTTTGTTGAT+TGG + chr8_2:2459080-2459099 Msa1227350:intron 20.0%
!!! TTTTGTTGATTGGATTATAA+TGG + chr8_2:2459090-2459109 Msa1227350:intron 20.0%
!!! TTTGTTGATTGGATTATAAT+GGG + chr8_2:2459091-2459110 Msa1227350:intron 20.0%
!!! TATTATTAGCATTTTGTCTA+TGG + chr8_2:2459217-2459236 Msa1227350:intron 20.0%
!!! ATTATTAGCATTTTGTCTAT+GGG + chr8_2:2459218-2459237 Msa1227350:intron 20.0%
!!! TTTGTATTTGTTTAGTTTGA+CGG + chr8_2:2459254-2459273 Msa1227350:intron 20.0%
!!! GTATTTGTTTTTTAGGAATT+TGG + chr8_2:2459284-2459303 Msa1227350:intron 20.0%
!!! GATGTTTAATGTGTTTTATA+AGG + chr8_2:2459354-2459373 Msa1227350:intron 20.0%
!!! ATGTGTTTTATAAGGTTATT+TGG + chr8_2:2459362-2459381 Msa1227350:intron 20.0%
!! GTTTGATTATTAGATTGAAT+TGG + chr8_2:2459441-2459460 Msa1227350:intron 20.0%
!! AGTTAATAAGTCAATTGAAA+TGG - chr8_2:2459595-2459614 None:intergenic 20.0%
!! TTTACAAGAACTTATGAAAA+TGG + chr8_2:2459651-2459670 Msa1227350:intron 20.0%
!!! GTTTTGAAGTGATTAATTAA+GGG + chr8_2:2459957-2459976 Msa1227350:intron 20.0%
!! ATTTGATAGAATTATGAAGT+TGG + chr8_2:2460011-2460030 Msa1227350:intron 20.0%
!! CAATTCTATCAAATAAACAA+AGG - chr8_2:2460356-2460375 None:intergenic 20.0%
!!! AAAGTGTTGTTATTTTTTAG+AGG + chr8_2:2460391-2460410 Msa1227350:intron 20.0%
!!! AAGTGTTGTTATTTTTTAGA+GGG + chr8_2:2460392-2460411 Msa1227350:intron 20.0%
!! TTTGCTTATATGATTGAATT+GGG + chr8_2:2460762-2460781 Msa1227350:intron 20.0%
!! TTTGAAAGAACTTATGAAAA+CGG + chr8_2:2460946-2460965 Msa1227350:intron 20.0%
!! ATAGAAAAAATGTAGATCTT+AGG + chr8_2:2461391-2461410 Msa1227350:intron 20.0%
!!! TTCAAGTGGTTATTTAATTT+TGG + chr8_2:2461541-2461560 Msa1227350:intron 20.0%
!!! CTTTTTGTTGTTGAATTAAA+AGG + chr8_2:2461844-2461863 Msa1227350:intron 20.0%
!!! GTGATTTTTTTTGTTTTGTT+TGG + chr8_2:2461995-2462014 Msa1227350:intron 20.0%
!!! GTGGTTTTGAATTTTTTAAT+TGG + chr8_2:2462050-2462069 Msa1227350:intron 20.0%
!!! TTAATTGGTTTCTTGTTTAT+AGG + chr8_2:2462065-2462084 Msa1227350:intron 20.0%
!! CATTATTATAAGGTTTAGTT+TGG + chr8_2:2462245-2462264 Msa1227350:three_prime_UTR 20.0%
!!! TTCCAAAATTGTCATTTTTA+AGG - chr8_2:2462336-2462355 None:intergenic 20.0%
!! TGAATGAGTGTGTAATTTTT+GGG - chr8_2:2458687-2458706 None:intergenic 25.0%
!! ATGAATGAGTGTGTAATTTT+TGG - chr8_2:2458688-2458707 None:intergenic 25.0%
! TGAAGTATATGAGATTTCTA+AGG + chr8_2:2458954-2458973 Msa1227350:CDS 25.0%
!!! TTGTTTTTTAGGAATTTGGT+AGG + chr8_2:2459288-2459307 Msa1227350:intron 25.0%
! AGATCTATTTATTCAGCATA+TGG - chr8_2:2459331-2459350 None:intergenic 25.0%
!!! TTGGCTTTGTGATATAATTT+AGG + chr8_2:2459394-2459413 Msa1227350:intron 25.0%
!! ACATAGAATGTCTTTTTCTA+TGG + chr8_2:2459532-2459551 Msa1227350:intron 25.0%
!!! TACGTTATTTCTACAACAAA+AGG - chr8_2:2459783-2459802 None:intergenic 25.0%
! CAAAAAAGAACATTATACGT+GGG - chr8_2:2459933-2459952 None:intergenic 25.0%
!!! GTATAATGTTCTTTTTTGGA+GGG + chr8_2:2459935-2459954 Msa1227350:intron 25.0%
!!! GGTTTTGAAGTGATTAATTA+AGG + chr8_2:2459956-2459975 Msa1227350:intron 25.0%
!!! AGAAGTGTTCTTTGTTTATT+TGG + chr8_2:2460088-2460107 Msa1227350:intron 25.0%
! TTAGCTTATTGATGTTTGAA+TGG + chr8_2:2460153-2460172 Msa1227350:intron 25.0%
!!! GAAAAGTGTTATTAGAAATG+TGG + chr8_2:2460236-2460255 Msa1227350:intron 25.0%
! TTTGATTAGGTAGAATGTAT+TGG + chr8_2:2460278-2460297 Msa1227350:intron 25.0%
! TTGATTAGGTAGAATGTATT+GGG + chr8_2:2460279-2460298 Msa1227350:intron 25.0%
!! TTATGTAGAATGCTTTCTTT+TGG + chr8_2:2460436-2460455 Msa1227350:intron 25.0%
!! TATGTAGAATGCTTTCTTTT+GGG + chr8_2:2460437-2460456 Msa1227350:intron 25.0%
!! GTTTGGACTAGATATAATTT+AGG + chr8_2:2460714-2460733 Msa1227350:intron 25.0%
! GTTTGCTTATATGATTGAAT+TGG + chr8_2:2460761-2460780 Msa1227350:intron 25.0%
!!! CTTATTTTCATAAACTCTCT+AGG + chr8_2:2460997-2461016 Msa1227350:intron 25.0%
! ATCTGATAGAATCATAAAGT+TGG + chr8_2:2461234-2461253 Msa1227350:intron 25.0%
!! ATTTAGAATGATTAGACTCA+TGG + chr8_2:2461331-2461350 Msa1227350:intron 25.0%
!! TTCGTAAAAGTGTAGATAAT+AGG + chr8_2:2461426-2461445 Msa1227350:intron 25.0%
!! TAAAAGTGTAGATAATAGGA+GGG + chr8_2:2461430-2461449 Msa1227350:intron 25.0%
! TGAATGAGTGAATGAAATTA+GGG + chr8_2:2461886-2461905 Msa1227350:intron 25.0%
!! GAATTTGATCAACAATTTTG+TGG + chr8_2:2462031-2462050 Msa1227350:intron 25.0%
! AAATAGTACTCAGTACTTAA+CGG - chr8_2:2462220-2462239 None:intergenic 25.0%
! AATAACAAGCAAAACTGAAA+TGG - chr8_2:2462299-2462318 None:intergenic 25.0%
!! GACCTTAAAAATGACAATTT+TGG + chr8_2:2462331-2462350 Msa1227350:three_prime_UTR 25.0%
! ATATATGATGTTACACCAAA+GGG - chr8_2:2462422-2462441 None:intergenic 25.0%
!! AAACTTCATTTTCTAAGCTT+AGG + chr8_2:2462577-2462596 Msa1227350:three_prime_UTR 25.0%
!! AACTTCATTTTCTAAGCTTA+GGG + chr8_2:2462578-2462597 Msa1227350:three_prime_UTR 25.0%
AACCTAAAACAGTTCATGTT+GGG + chr8_2:2458614-2458633 Msa1227350:five_prime_UTR 30.0%
!!! TTAAGCCCATGAACTTTTTT+GGG - chr8_2:2458645-2458664 None:intergenic 30.0%
!!! TTTAAGCCCATGAACTTTTT+TGG - chr8_2:2458646-2458665 None:intergenic 30.0%
!! CATTTTTGAGAATCTCTTTC+CGG - chr8_2:2458768-2458787 None:intergenic 30.0%
AGATTCTCAAAAATGGTTTC+CGG + chr8_2:2458773-2458792 Msa1227350:exon 30.0%
! ATATGAGATTTCTAAGGAAG+AGG + chr8_2:2458960-2458979 Msa1227350:CDS 30.0%
ATGAATACTAAACCTTCTCA+TGG + chr8_2:2458983-2459002 Msa1227350:CDS 30.0%
GTATAAAAAAACAGAGCAAG+TGG - chr8_2:2459193-2459212 None:intergenic 30.0%
! TTTTGTCTATGGGATTTCAT+TGG + chr8_2:2459228-2459247 Msa1227350:intron 30.0%
TTTGTCTATGGGATTTCATT+GGG + chr8_2:2459229-2459248 Msa1227350:intron 30.0%
!! GGTTATTTGGTGTTTGTAAT+TGG + chr8_2:2459375-2459394 Msa1227350:intron 30.0%
! TAGAATGTCTTTTTCTATGG+AGG + chr8_2:2459535-2459554 Msa1227350:intron 30.0%
! AGAATGTCTTTTTCTATGGA+GGG + chr8_2:2459536-2459555 Msa1227350:intron 30.0%
TTATGAAAATGGCTTATGAC+AGG + chr8_2:2459662-2459681 Msa1227350:intron 30.0%
CCAAAAAAGAACATTATACG+TGG - chr8_2:2459934-2459953 None:intergenic 30.0%
!!! CCACGTATAATGTTCTTTTT+TGG + chr8_2:2459931-2459950 Msa1227350:intron 30.0%
!!! CGTATAATGTTCTTTTTTGG+AGG + chr8_2:2459934-2459953 Msa1227350:intron 30.0%
TTGAATGTTGTGTTATGTTC+AGG + chr8_2:2460055-2460074 Msa1227350:intron 30.0%
AAACACCAAATTACCTTATG+GGG - chr8_2:2460129-2460148 None:intergenic 30.0%
CAAACACCAAATTACCTTAT+GGG - chr8_2:2460130-2460149 None:intergenic 30.0%
ACAAACACCAAATTACCTTA+TGG - chr8_2:2460131-2460150 None:intergenic 30.0%
! ACAAAACCACAAAACACTTT+TGG - chr8_2:2460471-2460490 None:intergenic 30.0%
!! ATTAGGAAGTGTTGATATTG+TGG + chr8_2:2460611-2460630 Msa1227350:intron 30.0%
!! GGTTATTTGGTGTTTGTAAT+TGG + chr8_2:2459375-2459394 Msa1227350:intron 30.0%
TAGTTTATCTAAACATGCCT+AGG + chr8_2:2461130-2461149 Msa1227350:intron 30.0%
TTGAAGTGATTAATGAATCC+AGG + chr8_2:2461179-2461198 Msa1227350:intron 30.0%
!! AACTTTAAAACAATGCATCC+TGG - chr8_2:2461200-2461219 None:intergenic 30.0%
TTGAATGTTGTGTTGTGTTA+TGG + chr8_2:2461281-2461300 Msa1227350:intron 30.0%
!!! GGTTTTTGAGAAGTGTTATT+TGG + chr8_2:2461306-2461325 Msa1227350:intron 30.0%
TAGATCTTAGGATCATAACA+AGG + chr8_2:2461403-2461422 Msa1227350:intron 30.0%
! GTAAAAGTGTAGATAATAGG+AGG + chr8_2:2461429-2461448 Msa1227350:intron 30.0%
!!! TTTTTTTAGCGCTATTCAAG+TGG + chr8_2:2461527-2461546 Msa1227350:intron 30.0%
GTTGAACGTCTTTGTATATT+TGG + chr8_2:2461579-2461598 Msa1227350:intron 30.0%
CTGAATGAGTGAATGAAATT+AGG + chr8_2:2461885-2461904 Msa1227350:intron 30.0%
AATAGTACTCAGTACTTAAC+GGG - chr8_2:2462219-2462238 None:intergenic 30.0%
!! GCTTGTTATTCTATGTTTGT+TGG + chr8_2:2462309-2462328 Msa1227350:three_prime_UTR 30.0%
AACACTATTGGAAAGTAAGT+TGG - chr8_2:2462397-2462416 None:intergenic 30.0%
TATATGATGTTACACCAAAG+GGG - chr8_2:2462421-2462440 None:intergenic 30.0%
CATATATGATGTTACACCAA+AGG - chr8_2:2462423-2462442 None:intergenic 30.0%
ACTATGAAGCTTATAGTTGT+TGG + chr8_2:2462467-2462486 Msa1227350:three_prime_UTR 30.0%
ATCTCAAATGCAGGATATTT+AGG - chr8_2:2462516-2462535 None:intergenic 30.0%
TCTATATTCATCTCAAATGC+AGG - chr8_2:2462525-2462544 None:intergenic 30.0%
TGAGATGAATATAGACTAAC+TGG + chr8_2:2462530-2462549 Msa1227350:three_prime_UTR 30.0%
GAGATGAATATAGACTAACT+GGG + chr8_2:2462531-2462550 Msa1227350:three_prime_UTR 30.0%
AGATGAATATAGACTAACTG+GGG + chr8_2:2462532-2462551 Msa1227350:three_prime_UTR 30.0%
! ACTTCATTTTCTAAGCTTAG+GGG + chr8_2:2462579-2462598 Msa1227350:three_prime_UTR 30.0%
GAACCTAAAACAGTTCATGT+TGG + chr8_2:2458613-2458632 Msa1227350:five_prime_UTR 35.0%
! TTGGGCTTTTCTACTTTGTT+TGG - chr8_2:2458669-2458688 None:intergenic 35.0%
CGGAAAGAGATTCTCAAAAA+TGG + chr8_2:2458766-2458785 Msa1227350:exon 35.0%
TGTGAAGAAATTTGTGCTTC+AGG + chr8_2:2458911-2458930 Msa1227350:CDS 35.0%
TCATGGTCCTATTCACTTTA+GGG + chr8_2:2459000-2459019 Msa1227350:CDS 35.0%
! ATGTAGCTGTTGAGTGTTTT+TGG + chr8_2:2459480-2459499 Msa1227350:intron 35.0%
! CTGTTTTCATTAGCTACTCT+TGG - chr8_2:2459723-2459742 None:intergenic 35.0%
! AGTGATTAATTAAGGGTGCA+TGG + chr8_2:2459964-2459983 Msa1227350:intron 35.0%
AATGTTGTGTTATGTTCAGG+AGG + chr8_2:2460058-2460077 Msa1227350:intron 35.0%
ATGTTGTGTTATGTTCAGGA+GGG + chr8_2:2460059-2460078 Msa1227350:intron 35.0%
AACACCAAATTACCTTATGG+GGG - chr8_2:2460128-2460147 None:intergenic 35.0%
!! TTGACGAGTGTTGTTTGATT+AGG + chr8_2:2460265-2460284 Msa1227350:intron 35.0%
ATTAGGTAGAATGTATTGGG+AGG + chr8_2:2460282-2460301 Msa1227350:intron 35.0%
TTAGGTAGAATGTATTGGGA+GGG + chr8_2:2460283-2460302 Msa1227350:intron 35.0%
!! TAGAATGCTTTCTTTTGGGA+GGG + chr8_2:2460441-2460460 Msa1227350:intron 35.0%
!! TTTGGTGTTTGTAATTGGCT+TGG + chr8_2:2459380-2459399 Msa1227350:intron 35.0%
!! TTGCTGTTGAGTGTTTTTCA+AGG + chr8_2:2460804-2460823 Msa1227350:intron 35.0%
AGACATTCTACACAATCACA+GGG - chr8_2:2460844-2460863 None:intergenic 35.0%
AAGACATTCTACACAATCAC+AGG - chr8_2:2460845-2460864 None:intergenic 35.0%
GCTTGATTATGCATGTTGTT+TGG + chr8_2:2460890-2460909 Msa1227350:intron 35.0%
TTATGAAAACGGCTTATGAC+AGG + chr8_2:2460957-2460976 Msa1227350:intron 35.0%
AAAAAGAGGACATTCTACCT+AGG - chr8_2:2461150-2461169 None:intergenic 35.0%
! TAGGTAGAATGTCCTCTTTT+TGG + chr8_2:2461149-2461168 Msa1227350:intron 35.0%
! TAGAATGTCCTCTTTTTGGA+GGG + chr8_2:2461153-2461172 Msa1227350:intron 35.0%
TTCAAAAACCCTCCAAAAAG+AGG - chr8_2:2461164-2461183 None:intergenic 35.0%
AATGTTGTGTTGTGTTATGG+AGG + chr8_2:2461284-2461303 Msa1227350:intron 35.0%
ATGTTGTGTTGTGTTATGGA+GGG + chr8_2:2461285-2461304 Msa1227350:intron 35.0%
AGACTCATGGTTGTCAAAAT+CGG + chr8_2:2461344-2461363 Msa1227350:intron 35.0%
ATACCCAAACGATCTTTAGT+GGG - chr8_2:2461373-2461392 None:intergenic 35.0%
TATACCCAAACGATCTTTAG+TGG - chr8_2:2461374-2461393 None:intergenic 35.0%
! ATGTAGAATGCTGTCTTTTG+AGG + chr8_2:2461472-2461491 Msa1227350:intron 35.0%
! TGTAGAATGCTGTCTTTTGA+GGG + chr8_2:2461473-2461492 Msa1227350:intron 35.0%
ATCCTCTTGATCTTGTCATA+TGG - chr8_2:2461767-2461786 None:intergenic 35.0%
ATATGACAAGATCAAGAGGA+TGG + chr8_2:2461766-2461785 Msa1227350:CDS 35.0%
AGAAGCTGCTCAATAAATTG+AGG + chr8_2:2462136-2462155 Msa1227350:CDS 35.0%
GAAGCTGCTCAATAAATTGA+GGG + chr8_2:2462137-2462156 Msa1227350:CDS 35.0%
TTAGAAAATGAAGTTTCCGC+GGG - chr8_2:2462573-2462592 None:intergenic 35.0%
CTTAGAAAATGAAGTTTCCG+CGG - chr8_2:2462574-2462593 None:intergenic 35.0%
CCAAGCCCAAAAAAGTTCAT+GGG + chr8_2:2458637-2458656 Msa1227350:five_prime_UTR 40.0%
!! TTTTGAGAATCTCTTTCCGG+CGG - chr8_2:2458765-2458784 None:intergenic 40.0%
TCAAAAATGGTTTCCGGATC+AGG + chr8_2:2458779-2458798 Msa1227350:exon 40.0%
GAAGAAATTTGTGCTTCAGG+TGG + chr8_2:2458914-2458933 Msa1227350:CDS 40.0%
AAGTGAATAGGACCATGAGA+AGG - chr8_2:2458998-2459017 None:intergenic 40.0%
CTCATGGTCCTATTCACTTT+AGG + chr8_2:2458999-2459018 Msa1227350:CDS 40.0%
!!! TAGCTGTTGAGTGTTTTTGG+AGG + chr8_2:2459483-2459502 Msa1227350:intron 40.0%
!!! AGCTGTTGAGTGTTTTTGGA+GGG + chr8_2:2459484-2459503 Msa1227350:intron 40.0%
ATGATACCCACAGATGCTAT+AGG + chr8_2:2459880-2459899 Msa1227350:intron 40.0%
!! TCATTACTGATTCCCCCATA+AGG + chr8_2:2460113-2460132 Msa1227350:intron 40.0%
GATTCCCCCATAAGGTAATT+TGG + chr8_2:2460121-2460140 Msa1227350:intron 40.0%
! GTAGAATGCTTTCTTTTGGG+AGG + chr8_2:2460440-2460459 Msa1227350:intron 40.0%
!!! GGTTTGCCAAAAGTGTTTTG+TGG + chr8_2:2460462-2460481 Msa1227350:intron 40.0%
CACAAGATCTAGACAACACA+AGG - chr8_2:2460640-2460659 None:intergenic 40.0%
!! GTAATTGGCTTGGTGATGTT+TGG + chr8_2:2460697-2460716 Msa1227350:intron 40.0%
! GTAGAATGTCCTCTTTTTGG+AGG + chr8_2:2461152-2461171 Msa1227350:intron 40.0%
GACTCATGGTTGTCAAAATC+GGG + chr8_2:2461345-2461364 Msa1227350:intron 40.0%
GATCCCACTAAAGATCGTTT+GGG + chr8_2:2461367-2461386 Msa1227350:intron 40.0%
! TGTTGTTAAGTGTTGCGTGA+TGG + chr8_2:2461629-2461648 Msa1227350:intron 40.0%
TTGATCTTGTCATATGGAGG+AGG - chr8_2:2461761-2461780 None:intergenic 40.0%
CTCTTGATCTTGTCATATGG+AGG - chr8_2:2461764-2461783 None:intergenic 40.0%
CTCCATATGACAAGATCAAG+AGG + chr8_2:2461762-2461781 Msa1227350:CDS 40.0%
! TGGTTCTTACTTGAGAGCAT+CGG - chr8_2:2461798-2461817 None:intergenic 40.0%
!! TAGGGTGTTGAGACTTCAAA+AGG + chr8_2:2461904-2461923 Msa1227350:intron 40.0%
!! AGGGTGTTGAGACTTCAAAA+GGG + chr8_2:2461905-2461924 Msa1227350:intron 40.0%
! GGTTTCTTGTTTATAGGAGC+TGG + chr8_2:2462071-2462090 Msa1227350:intron 40.0%
! TTCTTGTTTATAGGAGCTGG+AGG + chr8_2:2462074-2462093 Msa1227350:intron 40.0%
GGATTATTGATGCTGCCAAT+AGG + chr8_2:2462488-2462507 Msa1227350:three_prime_UTR 40.0%
CAGGATATTTAGGCACCTAT+TGG - chr8_2:2462506-2462525 None:intergenic 40.0%
! GGCCCAACATGAACTGTTTT+AGG - chr8_2:2458619-2458638 None:intergenic 45.0%
GCCAAGCCCAAAAAAGTTCA+TGG + chr8_2:2458636-2458655 Msa1227350:five_prime_UTR 45.0%
!!! CCCATGAACTTTTTTGGGCT+TGG - chr8_2:2458640-2458659 None:intergenic 45.0%
!! ACAGCTAGGGTTTGATGATG+AGG - chr8_2:2458721-2458740 None:intergenic 45.0%
GTGCGCCAAAAGATCTTAGA+GGG - chr8_2:2459028-2459047 None:intergenic 45.0%
AGTGCGCCAAAAGATCTTAG+AGG - chr8_2:2459029-2459048 None:intergenic 45.0%
TTGCGACCTATAGCATCTGT+GGG - chr8_2:2459889-2459908 None:intergenic 45.0%
TTTGCGACCTATAGCATCTG+TGG - chr8_2:2459890-2459909 None:intergenic 45.0%
GGATCCCACTAAAGATCGTT+TGG + chr8_2:2461366-2461385 Msa1227350:intron 45.0%
ATACCACACAAGACTAAGCG+TGG + chr8_2:2461695-2461714 Msa1227350:CDS 45.0%
!! GGTTCTTACTTGAGAGCATC+GGG - chr8_2:2461797-2461816 None:intergenic 45.0%
GGACACAAATACTGCTTGCT+CGG + chr8_2:2461926-2461945 Msa1227350:CDS 45.0%
AGTTGTCATCAGAGGTTGGA+TGG + chr8_2:2461951-2461970 Msa1227350:CDS 45.0%
TCAGAGGTTGGATGGAACTA+CGG + chr8_2:2461959-2461978 Msa1227350:CDS 45.0%
TATAGGAGCTGGAGGATAAG+AGG + chr8_2:2462082-2462101 Msa1227350:intron 45.0%
TTCCAATAGTGTTGCCCCTT+TGG + chr8_2:2462404-2462423 Msa1227350:three_prime_UTR 45.0%
CTTAGCGCATATGCCTGATC+CGG - chr8_2:2458795-2458814 None:intergenic 50.0%
TTGCGACAATGGAGGCAAGA+CGG - chr8_2:2458853-2458872 None:intergenic 50.0%
TCTTGCCTCCATTGTCGCAA+AGG + chr8_2:2458853-2458872 Msa1227350:CDS 50.0%
AAGCTCCTTTGCGACAATGG+AGG - chr8_2:2458861-2458880 None:intergenic 50.0%
GAGAAGCTCCTTTGCGACAA+TGG - chr8_2:2458864-2458883 None:intergenic 50.0%
GTCGCAAAGGAGCTTCTCAA+TGG + chr8_2:2458866-2458885 Msa1227350:CDS 50.0%
GGAGCTTCTCAATGGTCAGA+AGG + chr8_2:2458874-2458893 Msa1227350:CDS 50.0%
GCTTCTCAATGGTCAGAAGG+TGG + chr8_2:2458877-2458896 Msa1227350:CDS 50.0%
GAGGGAGCCCTAAAGTGAAT+AGG - chr8_2:2459010-2459029 None:intergenic 50.0%
!!! GGGCTCCCTCTAAGATCTTT+TGG + chr8_2:2459020-2459039 Msa1227350:CDS 50.0%
! ATCTTTTGGCGCACTGTTCG+TGG + chr8_2:2459034-2459053 Msa1227350:CDS 50.0%
! TCTTTTGGCGCACTGTTCGT+GGG + chr8_2:2459035-2459054 Msa1227350:CDS 50.0%
!! TCTCCACGCTTAGTCTTGTG+TGG - chr8_2:2461701-2461720 None:intergenic 50.0%
ACACAAGACTAAGCGTGGAG+AGG + chr8_2:2461700-2461719 Msa1227350:CDS 50.0%
GCGCGATTGAAGGTCTATGA+GGG + chr8_2:2461731-2461750 Msa1227350:CDS 50.0%
GGACAGTTGTCATCAGAGGT+TGG + chr8_2:2461947-2461966 Msa1227350:CDS 50.0%
GAACTACGGTGGCATCATCA+AGG + chr8_2:2461973-2461992 Msa1227350:CDS 50.0%
!! AGCAAGGACTTCAAGTTGGG+GGG - chr8_2:2462196-2462215 None:intergenic 50.0%
!! GAGCAAGGACTTCAAGTTGG+GGG - chr8_2:2462197-2462216 None:intergenic 50.0%
!! GGAGCAAGGACTTCAAGTTG+GGG - chr8_2:2462198-2462217 None:intergenic 50.0%
! GGGAGCAAGGACTTCAAGTT+GGG - chr8_2:2462199-2462218 None:intergenic 50.0%
CTCAGTACTTAACGGGAGCA+AGG - chr8_2:2462212-2462231 None:intergenic 50.0%
CACCAAAGGGGCAACACTAT+TGG - chr8_2:2462409-2462428 None:intergenic 50.0%
CATCAAACCCTAGCTGTCCC+CGG + chr8_2:2458724-2458743 Msa1227350:five_prime_UTR 55.0%
TTCTTCTCCGGGGACAGCTA+GGG - chr8_2:2458734-2458753 None:intergenic 55.0%
CGCGCGATTGAAGGTCTATG+AGG + chr8_2:2461730-2461749 Msa1227350:CDS 55.0%
GCTCGGACAGTTGTCATCAG+AGG + chr8_2:2461943-2461962 Msa1227350:CDS 55.0%
GAGGTTGGATGGAACTACGG+TGG + chr8_2:2461962-2461981 Msa1227350:CDS 55.0%
GGAGCTGGAGGATAAGAGGA+AGG + chr8_2:2462086-2462105 Msa1227350:intron 55.0%
GATAAGAGGAAGGAACGCGC+AGG + chr8_2:2462096-2462115 Msa1227350:CDS 55.0%
CGGGAGCAAGGACTTCAAGT+TGG - chr8_2:2462200-2462219 None:intergenic 55.0%
GTACTTTCACCTTGTGCCCG+CGG + chr8_2:2462554-2462573 Msa1227350:three_prime_UTR 55.0%
ATGAAGTTTCCGCGGGCACA+AGG - chr8_2:2462566-2462585 None:intergenic 55.0%
GTTCTTCTCCGGGGACAGCT+AGG - chr8_2:2458735-2458754 None:intergenic 60.0%
AGAATCTCTTTCCGGCGGCG+AGG - chr8_2:2458760-2458779 None:intergenic 60.0%
GAGGAAGGAACGCGCAGGTT+TGG + chr8_2:2462101-2462120 Msa1227350:CDS 60.0%
GGCGAGGCTGTTCTTCTCCG+GGG - chr8_2:2458744-2458763 None:intergenic 65.0%
CGGCGAGGCTGTTCTTCTCC+GGG - chr8_2:2458745-2458764 None:intergenic 65.0%
GCGGCGAGGCTGTTCTTCTC+CGG - chr8_2:2458746-2458765 None:intergenic 65.0%
GAGAAGAACAGCCTCGCCGC+CGG + chr8_2:2458746-2458765 Msa1227350:five_prime_UTR 65.0%
CAAGACGGCCGAGCATGTGG+TGG - chr8_2:2458838-2458857 None:intergenic 65.0%
AGGCAAGACGGCCGAGCATG+TGG - chr8_2:2458841-2458860 None:intergenic 65.0%
GGCTGCACTCGCGCGATTGA+AGG + chr8_2:2461721-2461740 Msa1227350:CDS 65.0%
GACGCCCGCCACCACATGCT+CGG + chr8_2:2458827-2458846 Msa1227350:CDS 70.0%
ACGGCCGAGCATGTGGTGGC+GGG - chr8_2:2458834-2458853 None:intergenic 70.0%
GACGGCCGAGCATGTGGTGG+CGG - chr8_2:2458835-2458854 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 2458600 2462608 2458600 ID=Msa1227350;Name=Msa1227350
chr8_2 mRNA 2458600 2462608 2458600 ID=Msa1227350-mRNA-1;Parent=Msa1227350;Name=Msa1227350-mRNA-1;_AED=0.11;_eAED=0.11;_QI=185|1|1|1|1|1|4|378|206
chr8_2 exon 2458600 2459056 2458600 ID=Msa1227350-mRNA-1:exon:655;Parent=Msa1227350-mRNA-1
chr8_2 exon 2461691 2461807 2461691 ID=Msa1227350-mRNA-1:exon:656;Parent=Msa1227350-mRNA-1
chr8_2 exon 2461907 2461994 2461907 ID=Msa1227350-mRNA-1:exon:657;Parent=Msa1227350-mRNA-1
chr8_2 exon 2462087 2462608 2462087 ID=Msa1227350-mRNA-1:exon:658;Parent=Msa1227350-mRNA-1
chr8_2 five_prime_UTR 2458600 2458784 2458600 ID=Msa1227350-mRNA-1:five_prime_utr;Parent=Msa1227350-mRNA-1
chr8_2 CDS 2458785 2459056 2458785 ID=Msa1227350-mRNA-1:cds;Parent=Msa1227350-mRNA-1
chr8_2 CDS 2461691 2461807 2461691 ID=Msa1227350-mRNA-1:cds;Parent=Msa1227350-mRNA-1
chr8_2 CDS 2461907 2461994 2461907 ID=Msa1227350-mRNA-1:cds;Parent=Msa1227350-mRNA-1
chr8_2 CDS 2462087 2462230 2462087 ID=Msa1227350-mRNA-1:cds;Parent=Msa1227350-mRNA-1
chr8_2 three_prime_UTR 2462231 2462608 2462231 ID=Msa1227350-mRNA-1:three_prime_utr;Parent=Msa1227350-mRNA-1
Gene Sequence

>Msa1227350

ATGGTTTCCGGATCAGGCATATGCGCTAAGAGAGTCGTGATCGACGCCCGCCACCACATGCTCGGCCGTCTTGCCTCCATTGTCGCAAAGGAGCTTCTCAATGGTCAGAAGGTGGTTCTTGTCAGATGTGAAGAAATTTGTGCTTCAGGTGGTCTTGTTAGACAGAAGATGAAGTATATGAGATTTCTAAGGAAGAGGATGAATACTAAACCTTCTCATGGTCCTATTCACTTTAGGGCTCCCTCTAAGATCTTTTGGCGCACTGTTCGTGGAATGATACCACACAAGACTAAGCGTGGAGAGGCTGCACTCGCGCGATTGAAGGTCTATGAGGGTATTCCTCCTCCATATGACAAGATCAAGAGGATGGTTGTTCCCGATGCTCTCAAGGTGTTGAGACTTCAAAAGGGACACAAATACTGCTTGCTCGGACAGTTGTCATCAGAGGTTGGATGGAACTACGGTGGCATCATCAAGGAGCTGGAGGATAAGAGGAAGGAACGCGCAGGTTTGGCTTACGAGAAAAAGAAGCTGCTCAATAAATTGAGGGCTAAAGCTGAGAAGATTGTAGACGAGAAGCTTGCCCCCCAACTTGAAGTCCTTGCTCCCGTTAAGTACTGA
Protein sequence

>Msa1227350

MVSGSGICAKRVVIDARHHMLGRLASIVAKELLNGQKVVLVRCEEICASGGLVRQKMKYMRFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGEAALARLKVYEGIPPPYDKIKRMVVPDALKVLRLQKGHKYCLLGQLSSEVGWNYGGIIKELEDKRKERAGLAYEKKKLLNKLRAKAEKIVDEKLAPQLEVLAPVKY*