AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1126690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126690 MtrunA17_Chr7g0268431 96.694 121 3 1 1 121 1 120 3.68e-84 244
Msa1126690 MtrunA17_Chr1g0176261 91.057 123 7 3 1 121 33 153 2.75e-74 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126690 AT2G37270.1 78.814 118 24 1 5 121 4 121 7.64e-66 199
Msa1126690 AT2G37270.2 78.814 118 24 1 5 121 4 121 7.64e-66 199
Msa1126690 AT3G11940.1 82.857 105 18 0 17 121 17 121 1.30e-64 196
Msa1126690 AT3G11940.2 82.857 105 18 0 17 121 17 121 1.30e-64 196

Find 40 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTCAATATATATCAATCAT+AGG 0.343232 7_3:+78249019 None:intergenic
GAGCTTGACTTCGTTTAGAT+TGG 0.348914 7_3:+78249776 None:intergenic
CGATAACTTGAATCGGGTTC+TGG 0.350343 7_3:+78249093 None:intergenic
ATGGAGCTTCGACGACGTTC+AGG 0.358685 7_3:-78249745 Msa1126690:intron
AGGAACATATGTTGCATGTT+TGG 0.372138 7_3:+78249280 None:intergenic
GGTACTCAAAGAAGCGCTTC+AGG 0.411900 7_3:-78249244 Msa1126690:CDS
CGCGGCCGTCAGCTTCTCCC+TGG 0.412002 7_3:+78249947 None:intergenic
AGCTTGACTTCGTTTAGATT+GGG 0.412691 7_3:+78249777 None:intergenic
GAAAGAAACTCATGGCTGTT+AGG 0.417495 7_3:-78249157 Msa1126690:CDS
CAGATTACCGATCTGTCTCT+TGG 0.434208 7_3:-78249326 Msa1126690:CDS
AGATTACCGATCTGTCTCTT+GGG 0.440013 7_3:-78249325 Msa1126690:CDS
TCGTTGATGCTGTCGTCAAC+AGG 0.441734 7_3:-78249073 Msa1126690:CDS
GCATCAACGATAACTTGAAT+CGG 0.455711 7_3:+78249086 None:intergenic
AGATTGGGATCAACTTCGAC+TGG 0.475242 7_3:+78249792 None:intergenic
CTGTCTCTTGGGGATTACAT+TGG 0.493111 7_3:-78249314 Msa1126690:CDS
GTCAATATATATCAATCATA+GGG 0.507828 7_3:+78249020 None:intergenic
ACATATGTTCCTCACACTGC+TGG 0.521105 7_3:-78249269 Msa1126690:CDS
GGGGATTACATTGGAGCAAG+AGG 0.537561 7_3:-78249305 Msa1126690:CDS
GAACGTCGTCGAAGCTCCAT+TGG 0.540472 7_3:+78249748 None:intergenic
TTAGTGAGCCTCTCAACAAT+GGG 0.543323 7_3:+78249209 None:intergenic
GTTAGTGAGCCTCTCAACAA+TGG 0.562680 7_3:+78249208 None:intergenic
CATCAACGATAACTTGAATC+GGG 0.564821 7_3:+78249087 None:intergenic
AAGTCAAGCTCTTCAACCAA+TGG 0.580728 7_3:-78249764 Msa1126690:CDS
CTCAGTGTCCCATTGTTGAG+AGG 0.591866 7_3:-78249217 Msa1126690:CDS
GATTGGGATCAACTTCGACT+GGG 0.607523 7_3:+78249793 None:intergenic
GCCGCGCCGCACTATCTACA+AGG 0.608102 7_3:-78249930 Msa1126690:intron
GATTACCGATCTGTCTCTTG+GGG 0.611070 7_3:-78249324 Msa1126690:CDS
GTAATCCCCAAGAGACAGAT+CGG 0.613626 7_3:+78249319 None:intergenic
TAGGATCATTAAGCATGCCA+TGG 0.620948 7_3:-78249138 Msa1126690:CDS
GGGAAAATAACAAAATGCAA+AGG 0.622403 7_3:+78249040 None:intergenic
AGCGACGGTTTCATCAGCCA+TGG 0.634868 7_3:+78249818 None:intergenic
TAGCAAGTGAATGATCTCCA+TGG 0.641966 7_3:+78249121 None:intergenic
ATGTTCCTCACACTGCTGGT+AGG 0.649707 7_3:-78249265 Msa1126690:CDS
AAACAACGGAAAGAAACTCA+TGG 0.660227 7_3:-78249165 Msa1126690:CDS
ATGATGCACGGTAGAAACAA+CGG 0.667166 7_3:-78249179 Msa1126690:CDS
ACTAACTCTCTCATGATGCA+CGG 0.667607 7_3:-78249191 Msa1126690:CDS
AAGCTACCTTGTAGATAGTG+CGG 0.671061 7_3:+78249924 None:intergenic
AACTTCGACTGGGATAGCGA+CGG 0.675722 7_3:+78249803 None:intergenic
GAGTACCTACCAGCAGTGTG+AGG 0.691601 7_3:+78249260 None:intergenic
ACCTTGTAGATAGTGCGGCG+CGG 0.782232 7_3:+78249929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCTATTTTCATAATTTATAT+TGG - chr7_3:78249450-78249469 Msa1126690:intron 15.0%
!! TAAAATTAAAAAACCCATTA+AGG + chr7_3:78249587-78249606 None:intergenic 15.0%
!!! GCTATTTTCATAATTTATAT+TGG - chr7_3:78249450-78249469 Msa1126690:intron 15.0%
!! TAAAATTAAAAAACCCATTA+AGG + chr7_3:78249587-78249606 None:intergenic 15.0%
! TAATTCAATGATTAGGGTTA+GGG + chr7_3:78249159-78249178 None:intergenic 25.0%
! ATAATTCAATGATTAGGGTT+AGG + chr7_3:78249160-78249179 None:intergenic 25.0%
! CGTGAATAATTCAATGATTA+GGG + chr7_3:78249165-78249184 None:intergenic 25.0%
! CAAATTATGACATGTTTGTT+GGG + chr7_3:78249505-78249524 None:intergenic 25.0%
! ACAAATTATGACATGTTTGT+TGG + chr7_3:78249506-78249525 None:intergenic 25.0%
!!! TATTTTCAAGTAACTCATGT+CGG - chr7_3:78249528-78249547 Msa1126690:intron 25.0%
! TCAAATAAGCCAATCTAAAT+AGG - chr7_3:78249560-78249579 Msa1126690:intron 25.0%
! AATCTAAATAGGTCCTTAAT+GGG - chr7_3:78249571-78249590 Msa1126690:intron 25.0%
! TAATTCAATGATTAGGGTTA+GGG + chr7_3:78249159-78249178 None:intergenic 25.0%
! ATAATTCAATGATTAGGGTT+AGG + chr7_3:78249160-78249179 None:intergenic 25.0%
! CGTGAATAATTCAATGATTA+GGG + chr7_3:78249165-78249184 None:intergenic 25.0%
! CAAATTATGACATGTTTGTT+GGG + chr7_3:78249505-78249524 None:intergenic 25.0%
! ACAAATTATGACATGTTTGT+TGG + chr7_3:78249506-78249525 None:intergenic 25.0%
!!! TATTTTCAAGTAACTCATGT+CGG - chr7_3:78249528-78249547 Msa1126690:intron 25.0%
! TCAAATAAGCCAATCTAAAT+AGG - chr7_3:78249560-78249579 Msa1126690:intron 25.0%
! AATCTAAATAGGTCCTTAAT+GGG - chr7_3:78249571-78249590 Msa1126690:intron 25.0%
GCGTGAATAATTCAATGATT+AGG + chr7_3:78249166-78249185 None:intergenic 30.0%
TCGTAGAATCTGAATCTAAT+AGG - chr7_3:78249336-78249355 Msa1126690:intron 30.0%
CATTAAGGACCTATTTAGAT+TGG + chr7_3:78249572-78249591 None:intergenic 30.0%
CAATCTAAATAGGTCCTTAA+TGG - chr7_3:78249570-78249589 Msa1126690:intron 30.0%
GGGAAAATAACAAAATGCAA+AGG + chr7_3:78249969-78249988 None:intergenic 30.0%
GCGTGAATAATTCAATGATT+AGG + chr7_3:78249166-78249185 None:intergenic 30.0%
TCGTAGAATCTGAATCTAAT+AGG - chr7_3:78249336-78249355 Msa1126690:intron 30.0%
CATTAAGGACCTATTTAGAT+TGG + chr7_3:78249572-78249591 None:intergenic 30.0%
CAATCTAAATAGGTCCTTAA+TGG - chr7_3:78249570-78249589 Msa1126690:intron 30.0%
GGGAAAATAACAAAATGCAA+AGG + chr7_3:78249969-78249988 None:intergenic 30.0%
AGCTTGACTTCGTTTAGATT+GGG + chr7_3:78249232-78249251 None:intergenic 35.0%
ACAAGTTGTTCATCCAAACA+GGG + chr7_3:78249362-78249381 None:intergenic 35.0%
AACAAGTTGTTCATCCAAAC+AGG + chr7_3:78249363-78249382 None:intergenic 35.0%
AGGAACATATGTTGCATGTT+TGG + chr7_3:78249729-78249748 None:intergenic 35.0%
AAACAACGGAAAGAAACTCA+TGG - chr7_3:78249841-78249860 Msa1126690:intron 35.0%
CATCAACGATAACTTGAATC+GGG + chr7_3:78249922-78249941 None:intergenic 35.0%
GCATCAACGATAACTTGAAT+CGG + chr7_3:78249923-78249942 None:intergenic 35.0%
AGCTTGACTTCGTTTAGATT+GGG + chr7_3:78249232-78249251 None:intergenic 35.0%
ACAAGTTGTTCATCCAAACA+GGG + chr7_3:78249362-78249381 None:intergenic 35.0%
AACAAGTTGTTCATCCAAAC+AGG + chr7_3:78249363-78249382 None:intergenic 35.0%
AGGAACATATGTTGCATGTT+TGG + chr7_3:78249729-78249748 None:intergenic 35.0%
AAACAACGGAAAGAAACTCA+TGG - chr7_3:78249841-78249860 Msa1126690:intron 35.0%
CATCAACGATAACTTGAATC+GGG + chr7_3:78249922-78249941 None:intergenic 35.0%
GCATCAACGATAACTTGAAT+CGG + chr7_3:78249923-78249942 None:intergenic 35.0%
TGCCTTCAGAACTAAAAACC+AGG - chr7_3:78249041-78249060 Msa1126690:CDS 40.0%
GCCTTCAGAACTAAAAACCA+GGG - chr7_3:78249042-78249061 Msa1126690:CDS 40.0%
!!! TCCCTGGTTTTTAGTTCTGA+AGG + chr7_3:78249046-78249065 None:intergenic 40.0%
AAGCTACCTTGTAGATAGTG+CGG + chr7_3:78249085-78249104 None:intergenic 40.0%
GTTAACGAGAGAGATGAAGA+AGG + chr7_3:78249133-78249152 None:intergenic 40.0%
TTGAATTATTCACGCAGCCA+TGG - chr7_3:78249171-78249190 Msa1126690:CDS 40.0%
GAGCTTGACTTCGTTTAGAT+TGG + chr7_3:78249233-78249252 None:intergenic 40.0%
AAGTCAAGCTCTTCAACCAA+TGG - chr7_3:78249242-78249261 Msa1126690:CDS 40.0%
TGAATCTAATAGGCCCTGTT+TGG - chr7_3:78249346-78249365 Msa1126690:intron 40.0%
AGATTACCGATCTGTCTCTT+GGG - chr7_3:78249681-78249700 Msa1126690:intron 40.0%
TTAGTGAGCCTCTCAACAAT+GGG + chr7_3:78249800-78249819 None:intergenic 40.0%
ACTAACTCTCTCATGATGCA+CGG - chr7_3:78249815-78249834 Msa1126690:CDS 40.0%
ATGATGCACGGTAGAAACAA+CGG - chr7_3:78249827-78249846 Msa1126690:intron 40.0%
GAAAGAAACTCATGGCTGTT+AGG - chr7_3:78249849-78249868 Msa1126690:intron 40.0%
TAGGATCATTAAGCATGCCA+TGG - chr7_3:78249868-78249887 Msa1126690:intron 40.0%
TAGCAAGTGAATGATCTCCA+TGG + chr7_3:78249888-78249907 None:intergenic 40.0%
TGCCTTCAGAACTAAAAACC+AGG - chr7_3:78249041-78249060 Msa1126690:CDS 40.0%
GCCTTCAGAACTAAAAACCA+GGG - chr7_3:78249042-78249061 Msa1126690:CDS 40.0%
!!! TCCCTGGTTTTTAGTTCTGA+AGG + chr7_3:78249046-78249065 None:intergenic 40.0%
AAGCTACCTTGTAGATAGTG+CGG + chr7_3:78249085-78249104 None:intergenic 40.0%
GTTAACGAGAGAGATGAAGA+AGG + chr7_3:78249133-78249152 None:intergenic 40.0%
TTGAATTATTCACGCAGCCA+TGG - chr7_3:78249171-78249190 Msa1126690:CDS 40.0%
GAGCTTGACTTCGTTTAGAT+TGG + chr7_3:78249233-78249252 None:intergenic 40.0%
AAGTCAAGCTCTTCAACCAA+TGG - chr7_3:78249242-78249261 Msa1126690:CDS 40.0%
TGAATCTAATAGGCCCTGTT+TGG - chr7_3:78249346-78249365 Msa1126690:intron 40.0%
AGATTACCGATCTGTCTCTT+GGG - chr7_3:78249681-78249700 Msa1126690:intron 40.0%
TTAGTGAGCCTCTCAACAAT+GGG + chr7_3:78249800-78249819 None:intergenic 40.0%
ACTAACTCTCTCATGATGCA+CGG - chr7_3:78249815-78249834 Msa1126690:CDS 40.0%
ATGATGCACGGTAGAAACAA+CGG - chr7_3:78249827-78249846 Msa1126690:intron 40.0%
GAAAGAAACTCATGGCTGTT+AGG - chr7_3:78249849-78249868 Msa1126690:intron 40.0%
TAGGATCATTAAGCATGCCA+TGG - chr7_3:78249868-78249887 Msa1126690:intron 40.0%
TAGCAAGTGAATGATCTCCA+TGG + chr7_3:78249888-78249907 None:intergenic 40.0%
AAAAACCAGGGAGAAGCTGA+CGG - chr7_3:78249054-78249073 Msa1126690:CDS 45.0%
GATTGGGATCAACTTCGACT+GGG + chr7_3:78249216-78249235 None:intergenic 45.0%
AGATTGGGATCAACTTCGAC+TGG + chr7_3:78249217-78249236 None:intergenic 45.0%
CAAGAGACAGATCGGTAATC+TGG + chr7_3:78249682-78249701 None:intergenic 45.0%
CAGATTACCGATCTGTCTCT+TGG - chr7_3:78249680-78249699 Msa1126690:intron 45.0%
GATTACCGATCTGTCTCTTG+GGG - chr7_3:78249682-78249701 Msa1126690:intron 45.0%
GTAATCCCCAAGAGACAGAT+CGG + chr7_3:78249690-78249709 None:intergenic 45.0%
CTGTCTCTTGGGGATTACAT+TGG - chr7_3:78249692-78249711 Msa1126690:intron 45.0%
ACATATGTTCCTCACACTGC+TGG - chr7_3:78249737-78249756 Msa1126690:intron 45.0%
GTTAGTGAGCCTCTCAACAA+TGG + chr7_3:78249801-78249820 None:intergenic 45.0%
CGATAACTTGAATCGGGTTC+TGG + chr7_3:78249916-78249935 None:intergenic 45.0%
AAAAACCAGGGAGAAGCTGA+CGG - chr7_3:78249054-78249073 Msa1126690:CDS 45.0%
GATTGGGATCAACTTCGACT+GGG + chr7_3:78249216-78249235 None:intergenic 45.0%
AGATTGGGATCAACTTCGAC+TGG + chr7_3:78249217-78249236 None:intergenic 45.0%
CAAGAGACAGATCGGTAATC+TGG + chr7_3:78249682-78249701 None:intergenic 45.0%
CAGATTACCGATCTGTCTCT+TGG - chr7_3:78249680-78249699 Msa1126690:intron 45.0%
GATTACCGATCTGTCTCTTG+GGG - chr7_3:78249682-78249701 Msa1126690:intron 45.0%
GTAATCCCCAAGAGACAGAT+CGG + chr7_3:78249690-78249709 None:intergenic 45.0%
CTGTCTCTTGGGGATTACAT+TGG - chr7_3:78249692-78249711 Msa1126690:intron 45.0%
ACATATGTTCCTCACACTGC+TGG - chr7_3:78249737-78249756 Msa1126690:intron 45.0%
GTTAGTGAGCCTCTCAACAA+TGG + chr7_3:78249801-78249820 None:intergenic 45.0%
CGATAACTTGAATCGGGTTC+TGG + chr7_3:78249916-78249935 None:intergenic 45.0%
AACTTCGACTGGGATAGCGA+CGG + chr7_3:78249206-78249225 None:intergenic 50.0%
GGGGATTACATTGGAGCAAG+AGG - chr7_3:78249701-78249720 Msa1126690:intron 50.0%
ATGTTCCTCACACTGCTGGT+AGG - chr7_3:78249741-78249760 Msa1126690:intron 50.0%
!! GGTACTCAAAGAAGCGCTTC+AGG - chr7_3:78249762-78249781 Msa1126690:CDS 50.0%
CTCAGTGTCCCATTGTTGAG+AGG - chr7_3:78249789-78249808 Msa1126690:CDS 50.0%
TCGTTGATGCTGTCGTCAAC+AGG - chr7_3:78249933-78249952 Msa1126690:five_prime_UTR 50.0%
AACTTCGACTGGGATAGCGA+CGG + chr7_3:78249206-78249225 None:intergenic 50.0%
GGGGATTACATTGGAGCAAG+AGG - chr7_3:78249701-78249720 Msa1126690:intron 50.0%
ATGTTCCTCACACTGCTGGT+AGG - chr7_3:78249741-78249760 Msa1126690:intron 50.0%
!! GGTACTCAAAGAAGCGCTTC+AGG - chr7_3:78249762-78249781 Msa1126690:CDS 50.0%
CTCAGTGTCCCATTGTTGAG+AGG - chr7_3:78249789-78249808 Msa1126690:CDS 50.0%
TCGTTGATGCTGTCGTCAAC+AGG - chr7_3:78249933-78249952 Msa1126690:five_prime_UTR 50.0%
ACCTTGTAGATAGTGCGGCG+CGG + chr7_3:78249080-78249099 None:intergenic 55.0%
AGCGACGGTTTCATCAGCCA+TGG + chr7_3:78249191-78249210 None:intergenic 55.0%
GAACGTCGTCGAAGCTCCAT+TGG + chr7_3:78249261-78249280 None:intergenic 55.0%
ATGGAGCTTCGACGACGTTC+AGG - chr7_3:78249261-78249280 Msa1126690:CDS 55.0%
GAGTACCTACCAGCAGTGTG+AGG + chr7_3:78249749-78249768 None:intergenic 55.0%
ACCTTGTAGATAGTGCGGCG+CGG + chr7_3:78249080-78249099 None:intergenic 55.0%
AGCGACGGTTTCATCAGCCA+TGG + chr7_3:78249191-78249210 None:intergenic 55.0%
GAACGTCGTCGAAGCTCCAT+TGG + chr7_3:78249261-78249280 None:intergenic 55.0%
ATGGAGCTTCGACGACGTTC+AGG - chr7_3:78249261-78249280 Msa1126690:CDS 55.0%
GAGTACCTACCAGCAGTGTG+AGG + chr7_3:78249749-78249768 None:intergenic 55.0%
GCCGCGCCGCACTATCTACA+AGG - chr7_3:78249076-78249095 Msa1126690:CDS 65.0%
GCCGCGCCGCACTATCTACA+AGG - chr7_3:78249076-78249095 Msa1126690:CDS 65.0%
CGCGGCCGTCAGCTTCTCCC+TGG + chr7_3:78249062-78249081 None:intergenic 75.0%
CGCGGCCGTCAGCTTCTCCC+TGG + chr7_3:78249062-78249081 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 78249031 78249997 78249031 ID=Msa1126690;Name=Msa1126690
chr7_3 mRNA 78249031 78249997 78249031 ID=Msa1126690-mRNA-1;Parent=Msa1126690;Name=Msa1126690-mRNA-1;_AED=0.05;_eAED=0.05;_QI=69|1|0.66|1|0.5|0.33|3|0|135
chr7_3 exon 78249031 78249345 78249031 ID=Msa1126690-mRNA-1:exon:21799;Parent=Msa1126690-mRNA-1
chr7_3 exon 78249746 78249840 78249746 ID=Msa1126690-mRNA-1:exon:21798;Parent=Msa1126690-mRNA-1
chr7_3 exon 78249931 78249997 78249931 ID=Msa1126690-mRNA-1:exon:21797;Parent=Msa1126690-mRNA-1
chr7_3 five_prime_UTR 78249931 78249997 78249931 ID=Msa1126690-mRNA-1:five_prime_utr;Parent=Msa1126690-mRNA-1
chr7_3 five_prime_UTR 78249839 78249840 78249839 ID=Msa1126690-mRNA-1:five_prime_utr;Parent=Msa1126690-mRNA-1
chr7_3 CDS 78249746 78249838 78249746 ID=Msa1126690-mRNA-1:cds;Parent=Msa1126690-mRNA-1
chr7_3 CDS 78249031 78249345 78249031 ID=Msa1126690-mRNA-1:cds;Parent=Msa1126690-mRNA-1
Gene Sequence

>Msa1126690

ATGGCTGATGAAACCGTCGCTATCCCAGTCGAAGTTGATCCCAATCTAAACGAAGTCAAGCTCTTCAACCAATGGAGCTTCGACGACGTTCAGATTACCGATCTGTCTCTTGGGGATTACATTGGAGCAAGAGGATCCAAACATGCAACATATGTTCCTCACACTGCTGGTAGGTACTCAAAGAAGCGCTTCAGGAAAGCTCAGTGTCCCATTGTTGAGAGGCTCACTAACTCTCTCATGATGCACGGTAGAAACAACGGAAAGAAACTCATGGCTGTTAGGATCATTAAGCATGCCATGGAGATCATTCACTTGCTAACTGACCAGAACCCGATTCAAGTTATCGTTGATGCTGTCGTCAACAGGTATGATGATGCCTTTGCATTTTGTTATTTTCCCTATGATTGA

Protein sequence

>Msa1126690

MADETVAIPVEVDPNLNEVKLFNQWSFDDVQITDLSLGDYIGARGSKHATYVPHTAGRYSKKRFRKAQCPIVERLTNSLMMHGRNNGKKLMAVRIIKHAMEIIHLLTDQNPIQVIVDAVVNRYDDAFAFCYFPYD*